Patent application title: MicroRNA-Based Methods and Compositions For the Diagnosis, Prognosis and Treatment of Breast Cancer
Inventors:
Carlo M. Croce (Columbus, OH, US)
George A. Calin (Pearland, TX, US)
George A. Calin (Pearland, TX, US)
Assignees:
THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
IPC8 Class: AC12Q168FI
USPC Class:
424450
Class name: Drug, bio-affecting and body treating compositions preparations characterized by special physical form liposomes
Publication date: 2014-05-29
Patent application number: 20140147495
Abstract:
The present invention provides novel methods and compositions for the
diagnosis, prognosis and treatment of breast cancer. The invention also
provides methods of identifying anti-breast cancer agents.Claims:
1. A method of diagnosing a breast cancer associated with one or more
prognostic markers in a subject, comprising: measuring the level of at
least one miR gene product in a breast cancer sample from said subject,
wherein an alteration in the level of the at least one miR gene product
in the test sample, relative to the level of a corresponding miR gene
product in a control sample, is indicative of the subject having a breast
cancer associated with the one or more prognostic markers, wherein the
breast cancer associated with one or more prognostic markers and the at
least one miR gene product are selected from the group consisting of: (i)
the breast cancer is a breast cancer associated with estrogen receptor
expression and the miR gene product is selected from the group consisting
of miR-26a, miR-26b, miR-102 (miR-29b), miR-30a-5p, miR-30b, miR-30c,
miR-30d, miR-185, miR-191, miR-206, miR-212, and combinations thereof;
(ii) the breast cancer is a breast cancer associated with progesterone
receptor expression and the miR gene product is selected from the group
consisting of let-7c, miR-26a, miR-29b, miR-30a-5p, miR-30b, miR-30c,
miR-30d, and combinations thereof; (iii) the breast cancer is a breast
cancer associated with positive lymph node metastasis and the miR gene
product is selected from the group consisting of let-7f-1, let-7a-3,
let-7a-2, miR-9-3, and combinations thereof; (iv) the breast cancer is a
breast cancer associated with a high proliferative index and the miR gene
product is selected from the group consisting of let-7c, let-7d, miR-26a,
miR-26b, miR-30a-5p, miR-102, miR-145, and combinations thereof; (v) the
breast cancer is a breast cancer associated with detectable p53
expression and the miR gene product is selected from the group consisting
of miR-16a, miR-128b and a combination thereof; (vi) the breast cancer is
a breast cancer associated with high vascular invasion and the miR gene
product is selected from the group consisting of miR-9-3, miR-10b,
miR-27a, miR-29a, miR-123, miR-205 and combinations thereof; and (vii)
the breast cancer is a breast cancer associated with an advanced tumor
stage and the miR gene product is selected from the group consisting of
miR-9-2, miR-15-a, miR-21, miR-30a-s, miR-133a-1, miR-137, miR-153-2,
miR-154, miR-181a, miR-203, miR-213, and combinations thereof.
2. The method of claim 1, further comprising: administering, to the subject, a pharmaceutical composition for treating breast cancer, wherein the pharmaceutical composition comprises: at least one miR expression inhibitor compound or at least one miR gene product and a pharmaceutically-acceptable carrier.
3. The method of claim 1, comprising: reverse transcribing RNA from a test sample obtained from the subject to provide a set of target oligodeoxynucleotides; hybridizing the target oligodeoxynucleotides to a microarray comprising miRNA-specific probe oligonucleotides to provide a hybridization profile for the test sample; and comparing the test sample hybridization profile to a hybridization profile generated from a control sample, wherein an alteration in the signal of at least one miRNA is indicative of the subject either having, or being at risk for developing, breast cancer.
4. A method of treating breast cancer in a subject who has a breast cancer in which at least one miR gene product is down-regulated or up-regulated in the cancer cells of the subject relative to control cells, comprising: (1) when the at least one miR gene product is down-regulated in the cancer cells, administering to the subject an effective amount of at least one isolated miR gene product, provided that the miR gene product is not miR-15a or miR-16-1, such that proliferation of cancer cells in the subject is inhibited; or (2) when the at least one miR gene product is up-regulated in the cancer cells, administering to the subject an effective amount of at least one compound for inhibiting expression of the at least one miR gene product, such that proliferation of cancer cells in the subject is inhibited.
5. The method of claim 4, wherein the at least one isolated miR gene product in step (1) is selected from the group consisting of miR-145, miR-10b, miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, miR-101-1, miR-143 and combinations thereof.
6. The method of claim 4 wherein the at least one miR gene product in step (2) is selected from the group consisting of: miR-21, miR-155, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-213, let-7i (let-7d-v2), miR-122a, miR-128b, miR-136, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210, miR-213 and combinations thereof.
7. The method of claim 4, further comprising: determining the amount of at least one miR gene product in breast cancer cells, relative to control cells; and altering the amount of miR gene product expressed in the breast cancer cells by: (i) administering to the subject an effective amount of at least one isolated miR gene product, provided that the miR gene product is not miR-15a or miR-16-1, if the amount of the miR gene product expressed in the cancer cells is less than the amount of the miR gene product expressed in control cells; or (ii) administering to the subject an effective amount of at least one compound for inhibiting expression of the at least one miR gene product, if the amount of the miR gene product expressed in the cancer cells is greater than the amount of the miR gene product expressed in control cells, such that proliferation of cancer cells in the subject is inhibited.
8. The method of claim 7, wherein the at least one isolated miR gene product in step (i) is selected from the group consisting of: miR-145, miR-10b, miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, miR-101-1, miR-143 and combinations thereof.
9. The method of claim 7, wherein the at least one miR gene product in step (ii) is selected from the group consisting of miR-21, miR-155, miR-009-1 (miR-131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-213, let-7i (let-7d-v2), miR-122a, miR-128b, miR-136, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210, miR-213 and combinations thereof.
10. A pharmaceutical composition for treating breast cancer, comprising at least one isolated miR gene product of claim 8 and a pharmaceutically-acceptable carrier.
11. A pharmaceutical composition for treating breast cancer, comprising at least one miR expression inhibitor compound of claim 9, and a pharmaceutically-acceptable carrier.
12. A method of diagnosing whether a subject has, or is at risk for developing, breast cancer, comprising measuring the level of at least one miR-125b gene product in a test sample from said subject, wherein a decrease in the level of the miR-125b gene product in the test sample, relative to the level of a corresponding miR-155 gene product in a control sample, is indicative of the subject either having, or being at risk for developing, breast cancer.
13. The method of claim 12, which further comprises measuring at least one miR-125b-1 gene product.
14. The method of claim 12, which further comprises measuring at least one miR-125b-2 gene product.
15. The method of claim 12, which further comprises measuring at least one miR-10b gene product.
16. The method of claim 12, which further comprises measuring at least one miR-145 gene product.
17. The method of claim 12, which further comprises measuring at least one miR-21 gene product.
18. The method of claim 12, wherein the level of the at least one miR-125b gene product is measured using Northern blot analysis.
19. The method of claim 12, wherein the level of the at least one miR-125b gene product in the test sample is less than the level of the corresponding miR-125b gene product in the control sample.
20. The method of claim 12, wherein the level of the at least one miR-125b gene product in the test sample is greater than the level of the corresponding miR-125b gene product in the control sample.
21. A method of treating cancer in a subject in need thereof, comprising administering to the subject an effective amount of a compound for inhibiting the expression of a gene encoding one or more gene products in the subject, wherein the gene products are selected from one or more of miR-155, miR-10b, and miR-125b; thereby inhibiting the proliferation of cancer cells in the subject.
22. The method of claim 21, wherein the compound for inhibiting the expression of a gene encoding the gene product is an antisense nucleic acid.
23. The method of claim 21, wherein the antisense nucleic acid is selected from the group consisting of a single-stranded RNA, a single-stranded DNA, a single-stranded RNA-DNA chimera and a single-stranded PNA.
24. The method of claim 21, wherein the antisense nucleic acid contains one or more modifications to the nucleic acid backbone, a sugar moiety, a base moiety or a combination thereof.
25. The method of claim 21, wherein the compound for inhibiting the expression of a gene encoding the gene product is a double-stranded RNA molecule having at least 90% sequence homology with the mature miRNAs of: miR-10b in SEQ ID NO:31, miR-125b-1 in SEQ ID NO: 118, miR-125b-2 in SEQ ID NO: 121, and miR-155 in SEQ ID NO:183.
26. The method of claim 25, wherein the double-stranded RNA molecule is about 17 to about 29 nucleotides in length.
27. The method of claim 21, wherein the compound for inhibiting the expression of the gene encoding the gene product is a ribozyme.
28. The method of claim 21, wherein the compound for inhibiting the expression of a gene encoding the gene product is formulated as a pharmaceutical composition comprising the compound or a pharmaceutically-acceptable salt thereof, and a pharmaceutically-acceptable carrier.
29. The method of claim 21, wherein the compound for inhibiting the expression of a gene encoding the gene product is administered to the subject orally, parenterally, by injection or infusion.
30. The method of claim 21, wherein the compound for inhibiting the expression of a gene encoding the gene product is administered to the subject by direct injection into a tumor in the subject.
31. The method of claim 21, wherein the compound for inhibiting the expression of a gene encoding the gene product is administered in combination with a delivery reagent.
32. The method of claim 31, wherein the delivery reagent is a liposome.
33. The method of claim 21, wherein the nucleic acid encoding the compound for inhibiting the expression of a gene encoding the gene product is a recombinant plasmid or viral vector.
34. A method for inhibiting the proliferation of cancer cells, comprising administering a compound for inhibiting the expression of a gene encoding a miR-155 gene product to the cancer cells, thereby arresting or slowing the growth of the cancer cells.
35. The method of claim 34, wherein the compound for inhibiting the expression of a gene encoding a miR-155 gene product is an antisense nucleic acid.
36. A method for inhibiting the proliferation of cancer cells, comprising administering a compound for increasing the expression of a gene encoding one or more of a miR-10b, miR-125b-1 or miR-125b-2 gene product to the cancer cells, thereby arresting or slowing the growth of the cancer cells.
37. The method of claim 36, wherein the compound for increasing the expression of a gene encoding one or more of a miR-10b, miR-125b-1 or miR-125b-2g gene product is a sense nucleic acid.
38. A method of treating a subject with a breast cancer, comprising: i) selecting a subject with the breast cancer; and, ii) administering to the subject an isolated nucleic acid molecule encoding at least one transcript of: miR-155, miR-10b, miR-125b-1, and miR-125b-2, thereby treating the subject.
Description:
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional application claiming the benefit of U.S. application Ser. No. 12/012,235, now U.S. Pat. No. ______, issued ______, 2014, which entered the National Phase on Jan. 31, 2008, from the International PCT Application No. US06/029889, filed Jul. 31, 2006, which claims the benefit of U.S. Provisional Application No. 60/704,464, filed Aug. 1, 2005. The disclosures of each of the aforementioned applications are incorporated herein by reference for all purposes.
BACKGROUND OF THE INVENTION
[0003] Breast cancer is a significant health problem for women in the United States and throughout the world. Although advances have been made in the detection and treatment of the disease, breast cancer remains the second leading cause of cancer-related deaths in women, affecting more than 180,000 women in the United States each year. For women in North America, the life-time odds of getting breast cancer are now one in eight.
[0004] No universally successful method for the treatment or prevention of breast cancer is currently available. Management of breast cancer currently relies on a combination of early diagnosis (for example, through routine breast screening procedures) and aggressive treatment, which may include one or more of a variety of treatments, such as surgery, radiotherapy, chemotherapy and hormone therapy. The course of treatment for a particular breast cancer is often selected based on a variety of prognostic parameters including an analysis of specific tumor markers.
[0005] Although the discovery of BRCA1 and BRCA2 were important steps in identifying key genetic factors involved in breast cancer, it has become clear that mutations in BRCA1 and BRCA2 account for only a fraction of inherited susceptibility to breast cancer. In spite of considerable research into therapies for breast cancer, breast cancer remains difficult to diagnose and treat effectively, and the high mortality observed in breast cancer patients indicates that improvements are needed in the diagnosis, treatment and prevention of the disease.
[0006] MicroRNAs are a class of small, non-coding RNAs that control gene expression by hybridizing to and triggering either translational repression or, less frequently, degradation of a messenger RNA (mRNA) target. The discovery and study of miRNAs has revealed miRNA-mediated gene regulatory mechanisms that play important roles in organismal development and various cellular processes, such as cell differentiation, cell growth and cell death. Recent studies suggest that aberrant expression of particular miRNAs may be involved in human diseases, such as neurological disorders and cancer. In particular, misexpression of miR-16-1 and/or miR-15a has been found in human chronic lymphocytic leukemias.
[0007] The development and use of microarrays containing all known human microRNAs has permitted a simultaneous analysis of the expression of every miRNA in a sample. These microRNA microarrays have not only been used to confirm that miR-16-1 is deregulated in human CLL cells, but also to generate miRNA expression signatures that are associated with well-defined clinico-pathological features of human CLL.
[0008] The use of microRNA microarrays to identify a group of microRNAs, which are differentially-expressed between normal cells and breast cancer cells (for example, an expression signature or expression profile), may help pinpoint specific miRNAs that are involved in breast cancer. Furthermore, the identification of putative targets of these miRNAs may help to unravel their pathogenic role. The present invention provides novel methods and compositions for the diagnosis, prognosis and treatment of breast cancer.
SUMMARY OF THE INVENTION
[0009] The present invention is based, in part, on the identification of a breast cancer-specific signature of miRNAs that are differentially-expressed in breast cancer cells, relative to normal control cells.
[0010] Accordingly, embodiments of the invention encompass methods of diagnosing whether a subject has, or is at risk for developing, breast cancer, comprising measuring the level of at least one miR gene product in a test sample from the subject and comparing the level of the miR gene product in the test sample to the level of a corresponding miR gene product in a control sample. An alteration (for example, an increase, a decrease) in the level of the miR gene product in the test sample, relative to the level of a corresponding miR gene product in a control sample, is indicative of the subject either having, or being at risk for developing, breast cancer. In certain embodiments, the at least one miR gene product is selected from the group consisting of miR-125b-1, miR125b-2, miR-145, miR-21, miR-155, miR-10b and combinations thereof.
[0011] The level of the at least one miR gene product can be measured using a variety of techniques that are well known to those of skill in the art. In one embodiment, the level of the at least one miR gene product is measured using Northern blot analysis. In another embodiment, the level of the at least one miR gene product is measured by reverse transcribing RNA from a test sample obtained from the subject to provide a set of target oligodeoxynucleotides, hybridizing the target oligodeoxynucleotides to a microarray that comprises miRNA-specific probe oligonucleotides to provide a hybridization profile for the test sample, and comparing the test sample hybridization profile to a hybridization profile generated from a control sample. An alteration in the signal of at least one miRNA in the test sample relative to the control sample is indicative of the subject either having, or being at risk for developing, breast cancer. In a particular embodiment, the microarray comprises miRNA-specific probe oligonucleotides for a substantial portion of the human miRNome. In a further embodiment, the microarray comprises miRNA-specific probe oligonucleotides for one or more miRNAs selected from the group consisting of miR-145, miR-21, miR-155, miR-10b, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, miR-213, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, let-7i (let-7d-v2), miR-101-1, miR-122a, miR-128b, miR-136, miR-143, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-205, miR-206, miR-210 and combinations thereof.
[0012] Embodiments of the invention also provide methods of diagnosing a breast cancer associated with one or more prognostic markers, comprising measuring the level of at least one miR gene product in a breast cancer test sample from a subject and comparing the level of the at least one miR gene product in the breast cancer test sample to the level of a corresponding miR gene product in a control sample. The breast cancer can be associated with one or more adverse prognostic markers associated with breast cancer, such as, but not limited to, estrogen receptor expression, progesterone receptor expression, positive lymph node metastasis, high proliferative index, detectable p53 expression, advanced tumor stage, and high vascular invasion. In one embodiment, the level of the at least one miR gene product is measured by reverse transcribing RNA from a test sample obtained from the subject to provide a set of target oligodeoxynucleotides, hybridizing the target oligodeoxynucleotides to a microarray that comprises miRNA-specific probe oligonucleotides to provide a hybridization profile for the test sample, and comparing the test sample hybridization profile to a hybridization profile generated from a control sample. An alteration in the signal of at least one miRNA in the test sample relative to the control sample is indicative of the subject either having, or being at risk for developing, a breast cancer associated with the one or more prognostic markers. In a particular embodiment, the microarray comprises at least one miRNA-specific probe oligonucleotide for a miRNA selected from the group consisting of miR-26a, miR-26b, miR-102 (miR-29b), miR-30a-5p, miR-30b, miR-30c, miR-30d, miR-185, miR-191, miR-206, miR-212, let-7c, miR-9-2, miR-15-a, miR-21, miR-30a-s, miR-133a-1, miR-137, miR-153-2, miR-154, miR-181a, miR-203, miR-213, let-7f-1, let-7a-3, let-7a-2, miR-9-3, miR-10b, miR-27a, miR-29a, miR-123, miR-205, let-7d, miR-145, miR-16a, miR-128b and combinations thereof.
[0013] Embodiments of the invention also encompass methods of treating breast cancer in a subject, wherein at least one miR gene product is de-regulated (for example, down-regulated, up-regulated) in the cancer cells of the subject. When the at least one isolated miR gene product is down-regulated in the breast cancer cells, the method comprises administering an effective amount of the at least one isolated miR gene product, such that proliferation of cancer cells in the subject is inhibited. In one embodiment, the method comprises administering an effective amount of the at least one isolated miR gene product, provided that the miR gene is not miR-15a or miR-16-1, such that proliferation of cancer cells in the subject is inhibited. When the at least one isolated miR gene product is up-regulated in the cancer cells, the method comprises administering to the subject an effective amount of at least one compound for inhibiting expression of the at least one miR gene, such that proliferation of breast cancer cells is inhibited.
[0014] In related embodiments, the invention provides methods of treating breast cancer in a subject, comprising determining the amount of at least one miR gene product in breast cancer cells from the subject, relative to control cells. If expression of the miR gene product is deregulated in breast cancer cells, the methods further comprise altering the amount of the at least one miR gene product expressed in the breast cancer cells. If the amount of the miR gene product expressed in the cancer cells is less than the amount of the miR gene product expressed in control cells, the method comprises administering an effective amount of at least one isolated miR gene product. In one embodiment, the miR gene product is not miR-15a or miR-16-1. If the amount of the miR gene product expressed in the cancer cells is greater than the amount of the miR gene product expressed in control cells, the method comprises administering to the subject an effective amount of at least one compound for inhibiting expression of the at least one miR gene. In one embodiment, the miR gene product is not miR-15a or miR-16-1.
[0015] The invention further provides pharmaceutical compositions for treating breast cancer. In one embodiment, the pharmaceutical compositions comprise at least one isolated miR gene product and a pharmaceutically-acceptable carrier. In a particular embodiment, the at least one miR gene product corresponds to a miR gene product that has a decreased level of expression in breast cancer cells relative to suitable control cells. In certain embodiments the isolated miR gene product is selected from the group consisting of miR-145, miR-10b, miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, miR-101-1, miR-143 and combinations thereof.
[0016] In another embodiment, the pharmaceutical compositions of the invention comprise at least one miR expression inhibition compound. In a particular embodiment, the at least one miR expression inhibition compound is specific for a miR gene whose expression is greater in breast cancer cells than control cells. In certain embodiments, the miR expression inhibition compound is specific for one or more miR gene products selected from the group consisting of miR-21, miR-155, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-213, let-7i (let-7d-v2), miR-122a, miR-128b, miR-136, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210, miR-213 and combinations thereof.
[0017] Embodiments of the invention also encompass methods of identifying an anti-breast cancer agent, comprising providing a test agent to a cell and measuring the level of at least one miR gene product in the cell. In one embodiment, the method comprises providing a test agent to a cell and measuring the level of at least one miR gene product associated with decreased expression levels in breast cancer cells. An increase in the level of the miR gene product in the cell, relative to a suitable control cell, is indicative of the test agent being an anti-breast cancer agent. In a particular embodiment, the at least one miR gene product associated with decreased expression levels in breast cancer cells is selected from the group consisting of miR-145, miR-10b, miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, miR-101-1, miR-143 and combinations thereof.
[0018] In other embodiments the method comprises providing a test agent to a cell and measuring the level of at least one miR gene product associated with increased expression levels in breast cancer cells. A decrease in the level of the miR gene product in the cell, relative to a suitable control cell, is indicative of the test agent being an anti-breast cancer agent. In a particular embodiment, at least one miR gene product associated with increased expression levels in breast cancer cells is selected from the group consisting of miR-21, miR-155, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-213, let-7i (let-7d-v2), miR-122a, miR-128b, miR-136, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210, miR-213 and combinations thereof.
BRIEF DESCRIPTION OF THE DRAWINGS
[0019] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
[0020] FIG. 1 depicts a tree generated by cluster analysis showing a separation of breast cancer from normal tissues on the basis of differential microRNA expression (P<0.05). The bar at the bottom of the figure indicates the group of cancer (red) or normal breast tissues (yellow).
[0021] FIG. 2 is a graph depicting the probability (0.0 to 1.0) of each sample being a cancerous or normal tissue based on PAM analysis. All breast cancer and normal tissues were correctly predicted by the miR signature shown in Table 2.
[0022] FIG. 3A is a Northern blot depicting the expression level of miR-125b, using a miR-125b complementary probe, in a normal sample, as well as several tumor samples from breast cancer patients (P). The U6 probe was used for normalization of expression levels for each sample.
[0023] FIG. 3B is a Northern blot depicting the expression level of miR-145, using a miR-145 complementary probe, in a normal sample, as well as several tumor samples from breast cancer patients (P). The U6 probe was used for normalization of expression levels for each sample.
[0024] FIG. 3C is a Northern blot depicting the expression level of miR-21, using a miR-21 complementary probe, in a normal sample, as well as several tumor samples from breast cancer patients (labeled as numbered patients). The U6 probe was used for normalization of expression levels for each sample.
[0025] FIG. 3D is a Northern blot depicting the expression levels of microRNAs miR-125b, miR-145 and miR-21 in various breast cancer cell lines. The expression level of each microRNA was also determined in a sample from normal tissues. The U6 probe was used for normalization of expression levels for each sample.
[0026] FIG. 4A is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with the presence (ER+) or absence (ER-) of estrogen receptor.
[0027] FIG. 4B is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with the presence (PR+) or absence (PR-) of progesterone receptor.
[0028] FIG. 4C is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with stage 1 (pT1) or stage 2 or 3 (pT2-3) tumors.
[0029] FIG. 4D is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with the presence (pN0) or absence (pN10+) of lymph node metastasis.
[0030] FIG. 4E is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with the presence or absence of vascular invasion.
[0031] FIG. 4F is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with a high (MIB-1>30) or low (MIB-1<20) proliferative index (PI).
[0032] FIG. 4G is a table listing miRNAs that are differentially-expressed in breast cancer samples associated with positive (p53+) or negative (p53-) immunostaining of p53.
DETAILED DESCRIPTION OF THE INVENTION
[0033] The present invention is based, in part, on the identification of particular miRNAs whose expression is altered in breast cancer cells relative to normal control cells, and microRNAs whose expression is altered in breast cancer cells associated with particular prognostic features, relative to breast cancer cells lacking such features.
[0034] As used herein interchangeably, a "miR gene product," "microRNA," "miR," or "miRNA" refers to the unprocessed or processed RNA transcript from an miR gene. As the miR gene products are not translated into protein, the term "miR gene products" does not include proteins. The unprocessed miR gene transcript is also called an "miR precursor," and typically comprises an RNA transcript of about 70-100 nucleotides in length. The miR precursor can be processed by digestion with an RNAse (for example, Dicer, Argonaut, or RNAse III, for example, E. coli RNAse III)) into an active 19-25 nucleotide RNA molecule. This active 19-25 nucleotide RNA molecule is also called the "processed" miR gene transcript or "mature" miRNA.
[0035] The active 19-25 nucleotide RNA molecule can be obtained from the miR precursor through natural processing routes (for example, using intact cells or cell lysates) or by synthetic processing routes (for example, using isolated processing enzymes, such as isolated Dicer, Argonaut, or RNAase III). It is understood that the active 19-25 nucleotide RNA molecule can also be produced directly by biological or chemical synthesis, without having been processed from the miR precursor.
[0036] The sequences of 187 miR gene products are provided in Table 1. All nucleic acid sequences herein are given in the 5' to 3' direction. In addition, genes are represented by italics, and gene products are represented by normal type; for example, mir-17 is the gene and miR-17 is the gene product.
[0037] The present invention encompasses methods of diagnosing whether a subject has, or is at risk for developing, breast cancer, comprising measuring the level of at least one miR gene product in a test sample from the subject and comparing the level of the miR gene product in the test sample to the level of a corresponding miR gene product in a control sample. As used herein, a "subject" can be any mammal that has, or is suspected of having, breast cancer. In a particular embodiment, the subject is a human who has, or is suspected of having, breast cancer.
[0038] The breast cancer can be any form of breast cancer and may be associated with one or more prognostic markers or features, including, but not limited to, estrogen receptor expression, progesterone receptor expression, lymph node metastasis, high proliferative index, detectable p53 expression, advanced tumor stage, and high vascular invasion. The prognostic marker can be associated with an adverse or negative prognosis, or it may be associated with a good or positive prognosis.
TABLE-US-00001 TABLE 1 Human miR Gene Product Sequences SEQ ID Name Precursor Sequence (5' to 3')* NO. hsa-let-7a-1-prec CACTGTGGGATGAGGTAGTAGGTTGTATAGTTTTAGGGTCACACCCACCACT 1 GGGAGATAACTATACAATCTACTGTCTTTCCTAACGTG hsa-let-7a-2-prec AGGTTGAGGTAGTAGGTTGTATAGTTTAGAATTACATCAAGGGAGATAACTG 2 TACAGCCTCCTAGCTTTCCT hsa-let-7a-3-prec GGGTGAGGTAGTAGGTTGTATAGTTTGGGGCTCTGCCCTGCTATGGGATAAC 3 TATACAATCTACTGTCTTTCCT hsa-let-7a-4-prec GTGACTGCATGCTCCCAGGTTGAGGTAGTAGGTTGTATAGTTTAGAATTACA 4 CAAGGGAGATAACTGTACAGCCTCCTAGCTTTCCTTGGGTCTTGCACTAAAC AAC hsa-let-7b-prec GGCGGGGTGAGGTAGTAGGTTGTGTGGTTTCAGGGCAGTGATGTTGCCCCTC 5 GGAAGATAACTATACAACCTACTGCCTTCCCTG hsa-let-7c-prec GCATCCGGGTTGAGGTAGTAGGTTGTATGGTTTAGAGTTACACCCTGGGAGT 6 TAACTGTACAACCTTCTAGCTTTCCTTGGAGC hsa-let-7d-prec CCTAGGAAGAGGTAGTAGGTTGCATAGTTTTAGGGCAGGGATTTTGCCCACA 7 AGGAGGTAACTATACGACCTGCTGCCTTTCTTAGG hsa-let-7d-v1- CTAGGAAGAGGTAGTAGTTTGCATAGTTTTAGGGCAAAGATTTTGCCCACAA 8 prec GTAGTTAGCTATACGACCTGCAGCCTTTTGTAG hsa-let-7d-v2- CTGGCTGAGGTAGTAGTTTGTGCTGTTGGTCGGGTTGTGACATTGCCCGCTGT 9 prec GGAGATAACTGCGCAAGCTACTGCCTTGCTAG hsa-let-7e-prec CCCGGGCTGAGGTAGGAGGTTGTATAGTTGAGGAGGACACCCAAGGAGATC 10 ACTATACGGCCTCCTAGCTTTCCCCAGG hsa-let-7f-1-prec TCAGAGTGAGGTAGTAGATTGTATAGTTGTGGGGTAGTGATTTTACCCTGTT 11 CAGGAGATAACTATACAATCTATTGCCTTCCCTGA hsa-let-7f-2-prec CTGTGGGATGAGGTAGTAGATTGTATAGTTGTGGGGTAGTGATTTTACCCTG 12 TTCAGGAGATAACTATACAATCTATTGCCTTCCCTGA hsa-let-7f-2-prec CTGTGGGATGAGGTAGTAGATTGTATAGTTTTAGGGTCATACCCCATCTTGG 13 AGATAACTATACAGTCTACTGTCTTTCCCACGG hsa-let-7g-prec TTGCCTGATTCCAGGCTGAGGTAGTAGTTTGTACAGTTTGAGGGTCTATGAT 14 ACCACCCGGTACAGGAGATAACTGTACAGGCCACTGCCTTGCCAGGAACAG CGCGC hsa-let-7i-prec CTGGCTGAGGTAGTAGTTTGTGCTGTTGGTCGGGTTGTGACATTGCCCGCTGT 15 GGAGATAACTGCGCAAGCTACTGCCTTGCTAG hsa-mir-001b-1- ACCTACTCAGAGTACATACTTCTTTATGTACCCATATGAACATACAATGCTAT 16 prec GGAATGTAAAGAAGTATGTATTTTTGGTAGGC hsa-mir-001b-1- CAGCTAACAACTTAGTAATACCTACTCAGAGTACATACTTCTTTATGTACCCA 17 prec TATGAACATACAATGCTATGGAATGTAAAGAAGTATGTATTTTTGGTAGGCA ATA hsa-mir-001b-2- GCCTGCTTGGGAAACATACTTCTTTATATGCCCATATGGACCTGCTAAGCTAT 18 prec GGAATGTAAAGAAGTATGTATCTCAGGCCGGG hsa-mir-001b- TGGGAAACATACTTCTTTATATGCCCATATGGACCTGCTAAGCTATGGAATG 19 prec TAAAGAAGTATGTATCTCA hsa-mir-001d- ACCTACTCAGAGTACATACTTCTTTATGTACCCATATGAACATACAATGCTAT 20 prec GGAATGTAAAGAAGTATGTATTTTTGGTAGGC hsa-mir-007-1 TGGATGTTGGCCTAGTTCTGTGTGGAAGACTAGTGATTTTGTTGTTTTTAGAT 21 AACTAAATCGACAACAAATCACAGTCTGCCATATGGCACAGGCCATGCCTCT ACA hsa-mir-007-1- TTGGATGTTGGCCTAGTTCTGTGTGGAAGACTAGTGATTTTGTTGTTTTTAGA 22 prec TAACTAAATCGACAACAAATCACAGTCTGCCATATGGCACAGGCCATGCCTC TACAG hsa-mir-007-2 CTGGATACAGAGTGGACCGGCTGGCCCCATCTGGAAGACTAGTGATTTTGTT 23 GTTGTCTTACTGCGCTCAACAACAAATCCCAGTCTACCTAATGGTGCCAGCC ATCGCA hsa-mir-007-2- CTGGATACAGAGTGGACCGGCTGGCCCCATCTGGAAGACTAGTGATTTTGTT 24 prec GTTGTCTTACTGCGCTCAACAACAAATCCCAGTCTACCTAATGGTGCCAGCC ATCGCA hsa-mir-007-3 AGATTAGAGTGGCTGTGGTCTAGTGCTGTGTGGAAGACTAGTGATTTTGTTG 25 TTCTGATGTACTACGACAACAAGTCACAGCCGGCCTCATAGCGCAGACTCCC TTCGAC hsa-mir-007-3- AGATTAGAGTGGCTGTGGTCTAGTGCTGTGTGGAAGACTAGTGATTTTGTTG 26 prec TTCTGATGTACTACGACAACAAGTCACAGCCGGCCTCATAGCGCAGACTCCC TTCGAC hsa-mir-009-1 CGGGGTTGGTTGTTATCTTTGGTTATCTAGCTGTATGAGTGGTGTGGAGTCTT 27 CATAAAGCTAGATAACCGAAAGTAAAAATAACCCCA hsa-mir-009-2 GGAAGCGAGTTGTTATCTTTGGTTATCTAGCTGTATGAGTGTATTGGTCTTCA 28 TAAAGCTAGATAACCGAAAGTAAAAACTCCTTCA hsa-mir-009-3 GGAGGCCCGTTTCTCTCTTTGGTTATCTAGCTGTATGAGTGCCACAGAGCCGT 29 CATAAAGCTAGATAACCGAAAGTAGAAATGATTCTCA hsa-mir-010a- GATCTGTCTGTCTTCTGTATATACCCTGTAGATCCGAATTTGTGTAAGGAATT 30 prec TTGTGGTCACAAATTCGTATCTAGGGGAATATGTAGTTGACATAAACACTCC GCTCT hsa-mir-010b- CCAGAGGTTGTAACGTTGTCTATATATACCCTGTAGAACCGAATTTGTGTGG 31 prec TATCCGTATAGTCACAGATTCGATTCTAGGGGAATATATGGTCGATGCAAAA ACTTCA hsa-mir-015a-2- GCGCGAATGTGTGTTTAAAAAAAATAAAACCTTGGAGTAAAGTAGCAGCAC 32 prec ATAATGGTTTGTGGATTTTGAAAAGGTGCAGGCCATATTGTGCTGCCTCAAA AATAC hsa-mir-015a- CCTTGGAGTAAAGTAGCAGCACATAATGGTTTGTGGATTTTGAAAAGGTGCA 33 prec GGCCATATTGTGCTGCCTCAAAAATACAAGG hsa-mir-015b- CTGTAGCAGCACATCATGGTTTACATGCTACAGTCAAGATGCGAATCATTAT 34 prec TTGCTGCTCTAG hsa-mir-015b- TTGAGGCCTTAAAGTACTGTAGCAGCACATCATGGTTTACATGCTACAGTCA 35 prec AGATGCGAATCATTATTTGCTGCTCTAGAAATTTAAGGAAATTCAT hsa-mir-016a- GTCAGCAGTGCCTTAGCAGCACGTAAATATTGGCGTTAAGATTCTAAAATTA 36 chr13 TCTCCAGTATTAACTGTGCTGCTGAAGTAAGGTTGAC hsa-mir-016b- GTTCCACTCTAGCAGCACGTAAATATTGGCGTAGTGAAATATATATTAAACA 37 chr3 CCAATATTACTGTGCTGCTTTAGTGTGAC hsa-mir-016- GCAGTGCCTTAGCAGCACGTAAATATTGGCGTTAAGATTCTAAAATTATCTC 38 prec-13 CAGTATTAACTGTGCTGCTGAAGTAAGGT hsa-mit-017-prec GTCAGAATAATGTCAAAGTGCTTACAGTGCAGGTAGTGATATGTGCATCTAC 39 TGCAGTGAAGGCACTTGTAGCATTATGGTGAC hsa-mir-018-prec TGTTCTAAGGTGCATCTAGTGCAGATAGTGAAGTAGATTAGCATCTACTGCC 40 CTAAGTGCTCCTTCTGGCA hsa-mir-018- TTTTTGTTCTAAGGTGCATCTAGTGCAGATAGTGAAGTAGATTAGCATCTACT 41 prec-13 GCCCTAAGTGCTCCTTCTGGCATAAGAA hsa-mir-019a- GCAGTCCTCTGTTAGTTTTGCATAGTTGCACTACAAGAAGAATGTAGTTGTG 42 prec CAAATCTATGCAAAACTGATGGTGGCCTGC hsa-mir-019a- CAGTCCTCTGTTAGTTTTGCATAGTTGCACTACAAGAAGAATGTAGTTGTGC 43 prec-13 AAATCTATGCAAAACTGATGGTGGCCTG hsa-mir-019b-1- CACTGTTCTATGGTTAGTTTTGCAGGTTTGCATCCAGCTGTGTGATATTCTGC 44 prec TGTGCAAATCCATGCAAAACTGACTGTGGTAGTG hsa-mir-019b-2- ACATTGCTACTTACAATTAGTTTTGCAGGTTTGCATTTCAGCGTATATATGTA 45 prec TATGTGGCTGTGCAAATCCATGCAAAACTGATTGTGATAATGT hsa-mir-019b- TTCTATGGTTAGTTTTGCAGGTTTGCATCCAGCTGTGTGATATTCTGCTGTGC 46 prec-13 AAATCCATGCAAAACTGACTGTGGTAG hsa-mir-019b- TTACAATTAGTTTTGCAGGTTTGCATTTCAGCGTATATATGTATATGTGGCTG 47 prec--X TGCAAATCCATGCAAAACTGATTGTGAT hsa-mir-020-prec GTAGCACTAAAGTGCTTATAGTGCAGGTAGTGTTTAGTTATCTACTGCATTAT 48 GAGCACTTAAAGTACTGC hsa-mir-021-prec TGTCGGGTAGCTTATCAGACTGATGTTGACTGTTGAATCTCATGGCAACACC 49 AGTCGATGGGCTGTCTGACA hsa-mir-021- ACCTTGTCGGGTAGCTTATCAGACTGATGTTGACTGTTGAATCTCATGGCAA 50 prec-17 CACCAGTCGATGGGCTGTCTGACATTTTG hsa-mir-022-prec GGCTGAGCCGCAGTAGTTCTTCAGTGGCAAGCTTTATGTCCTGACCCAGCTA 51 AAGCTGCCAGTTGAAGAACTGTTGCCCTCTGCC hsa-mir-023a- GGCCGGCTGGGGTTCCTGGGGATGGGATTTGCTTCCTGTCACAAATCACATT 52 prec GCCAGGGATTTCCAACCGACC hsa-mir-023b- CTCAGGTGCTCTGGCTGCTTGGGTTCCTGGCATGCTGATTTGTGACTTAAGAT 53 prec TAAAATCACATTGCCAGGGATTACCACGCAACCACGACCTTGGC hsa-mir-023- CCACGGCCGGCTGGGGTTCCTGGGGATGGGATTTGCTTCCTGTCACAAATCA 54 prec-19 CATTGCCAGGGATTTCCAACCGACCCTGA hsa-mir-024-1- CTCCGGTGCCTACTGAGCTGATATCAGTTCTCATTTTACACACTGGCTCAGTT 55 prec CAGCAGGAACAGGAG hsa-mir-024-2- CTCTGCCTCCCGTGCCTACTGAGCTGAAACACAGTTGGTTTGTGTACACTGGC 56 prec TCAGTTCAGCAGGAACAGGG hsa-mir-024- CCCTGGGCTCTGCCTCCCGTGCCTACTGAGCTGAAACACAGTTGGTTTGTGTA 57 prec-19 CACTGGCTCAGTTCAGCAGGAACAGGGG hsa-mir-024- CCCTCCGGTGCCTACTGAGCTGATATCAGTTCTCATTTTACACACTGGCTCAG 58 prec-9 TTCAGCAGGAACAGCATC hsa-mir-025-prec GGCCAGTGTTGAGAGGCGGAGACTTGGGCAATTGCTGGACGCTGCCCTGGG 59 CATTGCACTTGTCTCGGTCTGACAGTGCCGGCC hsa-mir-026a- AGGCCGTGGCCTCGTTCAAGTAATCCAGGATAGGCTGTGCAGGTCCCAATGG 60 prec CCTATCTTGGTTACTTGCACGGGGACGCGGGCCT hsa-mir-026b- CCGGGACCCAGTTCAAGTAATTCAGGATAGGTTGTGTGCTGTCCAGCCTGTT 61 prec CTCCATTACTTGGCTCGGGGACCGG hsa-mir-027a- CTGAGGAGCAGGGCTTAGCTGCTTGTGAGCAGGGTCCACACCAASGTCGTGTT 62 prec CACAGTGGCTAAGTTCCGCCCCCCAG hsa-mir-027b- AGGTGCAGAGCTTAGCTGATTGGTGAACAGTGATTGGTTTCCGCTTTGTTCA 63 prec CAGTGGCTAAGTTCTGCACCT hsa-mir-027b- ACCTCTCTAACAAGGTGCAGAGCTTAGCTGATTGGTGAACAGTGATTGGTTT 64 prec CCGCTTTGTTCACAGTGGCTAAGTTCTGCACCTGAAGAGAAGGTG hsa-mir-027- CCTGAGGAGCAGGGCTTAGCTGCTTGTGAGCAGGGTCCACACCAAGTCGTGT 65 prec-19 TCACAGTGGCTAAGTTCCGCCCCCCAGG hsa-mir-028-prec GGTCCTTGCCCTCAAGGAGCTCACAGTCTATTGAGTTACCTTTCTGACTTTCC 66 CACTAGATTGTGAGCTCCTGGAGGGCAGGCACT hsa-mir-029a-2 CCTTCTGTGACCCCTTAGAGGATGACTGATTTCTTTTGGTGTTCAGAGTCAAT 67 ATAATTTTCTAGCACCATCTGAAATCGGTTATAATGATTGGGGAAGAGCACC ATG hsa-mir-029a- ATGACTGATTTCTTTTGGTGTTCAGAGTCAATATAATTTTCTAGCACCATCTG 68 prec AAATCGGTTAT hsa-mir-029c- ACCACTGGCCCATCTCTTACACAGGCTGACCGATTTCTCCTGGTGTTCAGAGT 69 prec CTGTTTTTGTCTAGCACCATTTGAAATCGGTTATGATGTAGGGGGAAAAGCA GCAGC hsa-mir-030a- GCGACTGTAAACATCCTCGACTGGAAGCTGTGAAGCCACAGATGGGCTTTCA 70 prec GTCGGATGTTTGCAGCTGC hsa-mir-030b- ATGTAAACATCCTACACTCAGCTGTAATACATGGATTGGCTGGGAGGTGGAT 71 prec GTTTACGT hsa-mir-030b- ACCAAGTTTCAGTTCATGTAAACATCCTACACTCAGCTGTAATACATGGATT 72 prec GGCTGGGAGGTGGATGTTTACTTCAGCTGACTTGGA hsa-mir-030c- AGATACTGTAAACATCCTACACTCTCAGCTGTGGAAAGTAAGAAAGCTGGG 73 prec AGAAGGCTGTTTACTCTTTCT hsa-mir-030d- GTTGTTGTAAACATCCCCGACTGGAAGCTGTAAGACACAGCTAAGCTTTCAG 74 prec TCAGATGTTTGCTGCTAC hsa-mir-031-prec GGAGAGGAGGCAAGATGCTGGCATAGCTGTTGAACTGGGAACCTGCTATGC 75 CAACATATTGCCATCTTTCC hsa-mir-032-prec GGAGATATTGCACATTACTAAGTTGCATGTTGTCACGGCCTCAATGCAATTT 76 AGTGTGTGTGATATTTTC hsa-mir-033b- GGGGGCCGAGAGAGGCGGGCGGCCCCGCGGTGCATTGCTGTTGCATTGCAC 77 prec GTGTGTGAGGCGGGTGCAGTGCCTCGGCAGTGCAGCCCGGAGCCGGCCCCT
GGCACCAC hsa-mir-033-prec CTGTGGTGCATTGTAGTTGCATTGCATGTTCTGGTGGTACCCATGCAATGTTT 78 CCACAGTGCATCACAG hsa-mir-034-prec GGCCAGCTGTGAGTGTTTCTTTGGCAGTGTCTTAGCTGGTTGTTGTGAGCAAT 79 AGTAAGGAAGCAATCAGCAAGTATACTGCCCTAGAAGTGCTGCACGTTGTG GGGCCC hsa-mir-091- TCAGAATAATGTCAAAGTGCTTACAGTGCAGGTAGTGATATGTGCATCTACT 80 prec-13 GCAGTGAAGGCACTTGTAGCATTATGGTGA hsa-mir-092- CTTTCTACACAGGTTGGGATCGGTTGCAATGCTGTGTTTCTGTATGGTATTGC 81 prec-13 = 092-1 ACTTGTCCCGGCCTGTTGAGTTTGG hsa-mir-092- TCATCCCTGGGTGGGGATTTGTTGCATTACTTGTGTTCTATATAAAGTATTGC 82 prec-X = 092-2 ACTTGTCCCGGCCTGTGGAAGA hsa-mir-093- CTGGGGGCTCCAAAGTGCTGTTCGTGCAGGTAGTGTGATTACCCAACCTACT 83 prec-7.1 = 093-1 GCTGAGCTAGCACTTCCCGAGCCCCCGG hsa-mir-093- CTGGGGGCTCCAAAGTGCTGTTCGTGCAGGTAGTGTGATTACCCAACCTACT 84 prec-7.2 = 093-2 GCTGAGCTAGCACTTCCCGAGCCCCCGG hsa-mir-095- AACACAGTGGGCACTCAATAAATGTCTGTTGAATTGAAATGCGTTACATTCA 85 prec-4 ACGGGTATTTATTGAGCACCCACTCTGTG hsa-mir-096- TGGCCGATTTTGGCACTAGCACATTTTTGCTTGTGTCTCTCCGCTCTGAGCAA 86 prec-7 TCATGTGCAGTGCCAATATGGGAAA hsa-mir-098- GTGAGGTAGTAAGTTGTATTGTTGTGGGGTAGGGATATTAGGCCCCAATTAG 87 prec-X AAGATAACTATACAACTTACTACTTTCC hsa-mir-099b- GGCACCCACCCGTAGAACCGACCTTGCGGGGCCTTCGCCGCACACAAGCTCG 88 prec-19 TGTCTGTGGGTCCGTGTC hsa-mir-099- CCCATTGGCATAAACCCGTAGATCCGATCTTGTGGTGAAGTGGACCGCACAA 89 prec-21 GCTCGCTTCTATGGGTCTGTGTCAGTGTG hsa-mir-100-1/2- AAGAGAGAAGATATTGAGGCCTGTTGCCACAAACCCGTAGATCCGAACTTGT 90 prec GGTATTAGTCCGCACAAGCTTGTATCTATAGGTATGTGTCTGTTAGGCAATCT CAC hsa-mir-100- CCTGTTGCCACAAACCCGTAGATCCGAACTTGTGGTATTAGTCCGCACAAGC 91 prec-11 TTGTATCTATAGGTATGTGTCTGTTAGG hsa-mir-101-1/2- AGGCTGCCCTGGCTCAGTTATCACAGTGCTGATGCTGTCTATTCTAAAGGTA 92 prec CAGTACTGTGATAACTGAAGGATGGCAGCCATCTTACCTTCCATCAGAGGAG CCTCAC hsa-mir-101-prec TCAGTTATCACAGTGCTGATGCTGTCCATTCTAAAGGTACAGTACTGTGATA 93 ACTGA hsa-mir-101- TGCCCTGGCTCAGTTATCACAGTGCTGATGCTGTCTATTCTAAAGGTACAGTA 94 prec-1 CTGTGATAACTGAAGGATGGCA hsa-mir-101- TGTCCTTTTTCGGTTATCATGGTACCGATGCTGTATATCTGAAAGGTACAGTA 95 prec-9 CTGTGATAACTGAAGAATGGTG hsa-mir-102- CTTCTGGAAGCTGGTTTCACATGGTGGCTTAGATTTTTCCATCTTTGTATCTA 96 prec-1 GCACCATTTGAAATCAGTGTTTTAGGAG hsa-mir-102- CTTCAGGAAGCTGGTTTCATATGGTGGTTTAGATTTAAATAGTGATTGTCTAG 97 prec-7.1 CACCATTTGAAATCAGTGTTCTTGGGGG hsa-mir-102- CTTCAGGAAGCTGGTTTCATATGGTGGTTTAGATTTAAATAGTGATTGTCTAG 98 prec-7.2 CACCATTTGAAATCAGTGTTCTTGGGGG hsa-mir-103-2- TTGTGCTTTCAGCTTCTTTACAGTGCTGCCTTGTAGCATTCAGGTCAAGCAAC 99 prec ATTGTACAGGGCTATGAAAGAACCA hsa-mir-103- TTGTGCTTTCAGCTTCTTTACAGTGCTGCCTTGTAGCATTCAGGTCAAGCAAC 100 prec-20 ATTGTACAGGGCTATGAAAGAACCA hsa-mir-103- TACTGCCCTCGGCTTCTTTACAGTGCTGCCTTGTTGCATATGGATCAAGCAGC 101 prec-5 = 103-1 ATTGTACAGGGCTATGAAGGCATTG hsa-mir-104- AAATGTCAGACAGCCCATCGACTGGTGTTGCCATGAGATTCAACAGTCAACA 102 prec-17 TCAGTCTGATAAGCTACCCGACAAGG hsa-mir-105- TGTGCATCGTGGTCAAATGCTCAGACTCCTGTGGTGGCTGCTCATGCACCAC 103 prec-X.1 = 105-1 GGATGTTTGAGCATGTGCTACGGTGTCTA hsa-mir-105- TGTGCATCGTGGTCAAATGCTCAGACTCCTGTGGTGGCTGCTCATGCACCAC 104 prec-X.2 = 105-2 GGATGTTTGAGCATGTGCTACGGTGTCTA hsa-mir-106- CCTTGGCCATGTAAAAGTGCTTACAGTGCAGGTAGCTTTTTGAGATCTACTG 105 prec-X CAATGTAAGCACTTCTTACATTACCATGG hsa-mir-107- CTCTCTGCTTTCAGCTTCTTTACAGTGTTGCCTTGTGGCATGGAGTTCAAGCA 106 prec-10 GCATTGTACAGGGCTATCAAAGCACAGA hsa-mir-122a- CCTTAGCAGAGCTGTGGAGTGTGACAATGGTGTTTGTGTCTAAACTATCAAA 107 prec CGCCATTATCACACTAAATAGCTACTGCTAGGC hsa-mir-122a- AGCTGTGGAGTGTGACAATGGTGTTTGTGTCCAAACTATCAAACGCCATTAT 108 prec CACACTAAATAGCT hsa-mir-123-prec ACATTATTACTTTTGGTACGCGCTGTGACACTTCAAACTCGTACCGTGAGTAA 109 TAATGCGC hsa-mir-124a-1- tccttcctCAGGAGAAAGGCCTCTCTCTCCGTGTTCACAGCGGACCTTGATTTAAA 110 prec TGTCCATACAATTAAGGCACGCGGTGAATGCCAAGAATGGGGCT hsa-mir-124a-1- AGGCCTCTCTCTCCGTGTTCACAGCGGACCTTGATTTAAATGTCCATACAATT 111 prec AAGGCACGCGGTGAATGCCAAGAATGGGGCTG hsa-mir-124a-2- ATCAAGATTAGAGGCTCTGCTCTCCGTGTTCACAGCGGACCTTGATTTAATGT 112 prec CATACAATTAAGGCACGCGGTGAATGCCAAGAGCGGAGCCTACGGCTGCAC TTGAAG hsa-mir-124a-3- CCCGCCCCAGCCCTGAGGGCCCCTCTGCGTGTTCACAGCGGACCTTGATTTA 113 prec ATGTCTATACAATTAAGGCACGCGGTGAATGCCAAGAGAGGCGCCTCCGCC GCTCCTT hsa-mir-124a-3- TGAGGGCCCCTCTGCGTGTTCACAGCGGACCTTGATTTAATGTCTATACAATT 114 prec AAGGCACGCGGTGAATGCCAAGAGAGGCGCCTCC hsa-mir-124a- CTCTGCGTGTTCACAGCGGACCTTGATTTAATGTCTATACAATTAAGGCACG 115 prec CGGTGAATGCCAAGAG hsa-mir-124b- CTCTCCGTGTTCACAGCGGACCTTGATTTAATGTCATACAATTAAGGCACGC 116 prec GGTGAATGCCAAGAG hsa-mir-125a- TGCCAGTCTCTAGGTCCCTGAGACCCTTTAACCTGTGAGGACATCCAGGGTC 117 prec ACAGGTGAGGTTCTTGGGAGCCTGGCGTCTGGCC hsa-mir-125a- GGTCCCTGAGACCCTTTAACCTGTGAGGACATCCAGGGTCACAGGTGAGGTT 118 prec CTTGGGAGCCTGG hsa-mir-125b-1 ACATTGTTGCGCTCCTCTCAGTCCCTGAGACCCTAACTTGTGATGTTTACCGT 119 TTAAATCCACGGGTTAGGCTCTTGGGAGCTGCGAGTCGTGCTTTTGCATCCTG GA hsa-mir-125b-1 TGCGCTCCTCTCAGTCCCTGAGACCCTAACTTGTGATGTTTACCGTTTAAATC 120 CACGGGTTAGGCTCTTGGGAGCTGCGAGTCGTGCT hsa-mir-125b-2- ACCAGACTTTTCCTAGTCCCTGAGACCCTAACTTGTGAGGTATTTTAGTAACA 121 prec TCACAAGTCAGGCTCTTGGGACCTAGGCGGAGGGGA hsa-mir-125b-2- CCTAGTCCCTGAGACCCTAACTTGTGAGGTATTTTAGTAACATCACAAGTCA 122 prec GGCTCTTGGGACCTAGGC hsa-mir-126-prec CGCTGGCGACGGGACATTATTACTTTTGGTACGCGCTGTGACACTTCAAACT 123 CGTACCGTGAGTAATAATGCGCCGTCCACGGCA hsa-mir-126-prec ACATTATTACTTTTGGTACGCGCTGTGACACTTCAAACTCGTACCGTGAGTAA 124 TAATGCGC hsa-mir-127-prec TGTGATCACTGTCTCCAGCCTGCTGAAGCTCAGAGGGCTCTGATTCAGAAAG 125 ATCATCGGATCCGTCTGAGCTTGGCTGGTCGGAAGTCTCATCATC hsa-mir-127-prec CCAGCCTGCTGAAGCTCAGAGGGCTCTGATTCAGAAAGATCATCGGATCCGT 126 CTGAGCTTGGCTGGTCGG hsa-mir-128a- TGAGCTGTTGGATTCGGGGCCGTAGCACTGTCTGAGAGGTTTACATTTCTCA 127 prec CAGTGAACCGGTCTCTTTTTCAGCTGCTTC hsa-mir-128b- GCCCGGCAGCCACTGTGCAGTGGGAAGGGGGGCCGATACACTGTACGAGAG 128 prec TGAGTAGCAGGTCTCACAGTGAACCGGTCTCTTTCCCTACTGTGTCACACTCC TAATGG hsa-mir-128-prec GTTGGATTCGGGGCCGTAGCACTGTCTGAGAGGTTTACATTTCTCACAGTGA 129 ACCGGTCTCTTTTTCAGC hsa-mir-129-prec TGGATCTTTTTGCGGTCTGGGCTTGCTGTTCCTCTCAACAGTAGTCAGGAAGC 130 CCTTACCCCAAAAAGTATCTA hsa-mir-130a- TGCTGCTGGCCAGAGCTCTTTTCACATTGTGCTACTGTCTGCACCTGTCACTA 131 prec GCAGTGCAATGTTAAAAGGGCATTGGCCGTGTAGTG hsa-mir-131-1- gccaggaggcggGGTTGGTTGTTATCTTTGGTTATCTAGCTGTATGAGTGGTGTGG 132 prec AGTCTTCATAAAGCTAGATAACCGAAAGTAAAAATAACCCCATACACTGCGC AG hsa-mir-131-3- CACGGCGCGGCAGCGGCACTGGCTAAGGGAGGCCCGTTTCTCTCTTTGGTTA 133 prec TCTAGCTGTATGAGTGCCACAGAGCCGTCATAAAGCTAGATAACCGAAAGTA GAAATG hsa-mir-131-prec GTTGTTATCTTTGGTTATCTAGCTGTATGAGTGTATTGGTCTTCATAAAGCTA 134 GATAACCGAAAGTAAAAAC hsa-mir-132-prec CCGCCCCCGCGTCTCCAGGGCAACCGTGGCTTTCGATTGTTACTGTGGGAAC 135 TGGAGGTAACAGTCTACAGCCATGGTCGCCCCGCAGCACGCCCACGCGC hsa-mir-132-prec GGGCAACCGTGGCTTTCGATTGTTACTGTGGGAACTGGAGGTAACAGTCTAC 136 AGCCATGGTCGCCC hsa-mir-133a-1 ACAATGCTTTGCTAGAGCTGGTAAAATGGAACCAAATCGCCTCTTCAATGGA 137 TTTGGTCCCCTTCAACCAGCTGTAGCTATGCATTGA hsa-mir-133a-2 GGGAGCCAAATGCTTTGCTAGAGCTGGTAAAATGGAACCAAATCGACTGTCC 138 AATGGATTTGGTCCCCTTCAACCAGCTGTAGCTGTGCATTGATGGCGCCG hsa-mir-133-prec GCTAGAGCTGGTAAAATGGAACCAAATCGCCTCTTCAATGGATTTGGTCCCC 139 TTCAACCAGCTGTAGC hsa-mir-134-prec CAGGGTGTGTGACTGGTTGACCAGAGGGGCATGCACTGTGTTCACCCTGTGG 140 GCCACCTAGTCACCAACCCTC hsa-mir-134-prec AGGGTGTGTGACTGGTTGACCAGAGGGGCATGCACTGTGTTCACCCTGTGGG 141 CCACCTAGTCACCAACCCT hsa-mir-135-1- AGGCCTCGCTGTTCTCTATGGCTTTTTATTCCTATGTGATTCTACTGCTCACTC 142 prec ATATAGGGATTGGAGCCGTGGCGCACGGCGGGGACA hsa-mir-135-2- AGATAAATTCACTCTAGTGCTTTATGGCTTTTTATTCCTATGTGATAGTAATA 143 prec AAGTCTCATGTAGGGATGGAAGCCATGAAATACATTGTGAAAAATCA hsa-mir-135-prec CTATGGCTTTTTATTCCTATGTGATTCTACTGCTCACTCATATAGGGATTGGA 144 GCCGTGG hsa-mir-136-prec TGAGCCCTCGGAGGACTCCATTTGTTTTGATGATGGATTCTTATGCTCCATCA 145 TCGTCTCAAATGAGTCTTCAGAGGGTTCT hsa-mir-136-prec GAGGACTCCATTTGTTTTGATGATGGATTCTTATGCTCCATCATCGTCTCAAA 146 TGAGTCTTC hsa-mir-137-prec CTTCGGTGACGGGTATTCTTGGGTGGATAATACGGATTACGTTGTTATTGCTT 147 AAGAATACGCGTAGTCGAGG hsa-mir-138-1- CCCTGGCATGGTGTGGTGGGGCAGCTGGTGTTGTGAATCAGGCCGTTGCCAA 148 prec TCAGAGAACGGCTACTTCACAACACCAGGGCCACACCACACTACAGG hsa-mir-138-2- CGTTGCTGCAGCTGGTGTTGTGAATCAGGCCGACGAGCAGCGCATCCTCTTA 149 prec CCCGGCTATTTCACGACACCAGGGTTGCATCA hsa-mir-138-prec CAGCTGGTGTTGTGAATCAGGCCGACGAGCAGCGCATCCTCTTACCCGGCTA 150 TTTCACGACACCAGGGTTG hsa-mir-139-prec GTGTATTCTACAGTGCACGTGTCTCCAGTGTGGCTCGGAGGCTGGAGACGCG 151 GCCCTGTTGGAGTAAC hsa-mir-140 TGTGTCTCTCTCTGTGTCCTGCCAGTGGTTTTACCCTATGGTAGGTTACGTCA 152 TGCTGTTCTACCACAGGGTAGAACCACGGACAGGATACCGGGGCACC hsa-mir-140as- TCCTGCCAGTGGTTTTACCCTATGGTAGGTTACGTCATGCTGTTCTACCACAG 153 prec GGTAGAACCACGGACAGGA hsa-mir-140s- CCTGCCAGTGGTTTTACCCTATGGTAGGTTACGTCATGCTGTTCTACCACAGG 154 prec GTAGAACCACGGACAGG
hsa-mir-141-prec CGGCCGGCCCTGGGTCCATCTTCCAGTACAGTGTTGGATGGTCTAATTGTGA 155 AGCTCCTAACACTGTCTGGTAAAGATGGCTCCCGGGTGGGTTC hsa-mir-141-prec GGGTCCATCTTCCAGTACAGTGTTGGATGGTCTAATTGTGAAGCTCCTAACA 156 CTGTCTGGTAAAGATGGCCC hsa-mir-142as- ACCCATAAAGTAGAAAGCACTACTAACAGCACTGGAGGGTGTAGTGTTTCCT 157 prec ACTTTATGGATG hsa-mir-142-prec GACAGTGCAGTCACCCATAAAGTAGAAAGCACTACTAACAGCACTGGAGGG 158 TGTAGTGTTTCCTACTTTATGGATGAGTGTACTGTG hsa-mir-142s- ACCCATAAAGTAGAAAGCACTACTAACAGCACTGGAGGGTGTAGTGTTTCCT 159 prec ACTTTATGGATG hsa-mir-143-prec GCGCAGCGCCCTGTCTCCCAGCCTGAGGTGCAGTGCTGCATCTCTGGTCAGT 160 TGGGAGTCTGAGATGAAGCACTGTAGCTCAGGAAGAGAGAAGTTGTTCTGC AGC hsa-mir-143-prec CCTGAGGTGCAGTGCTGCATCTCTGGTCAGTTGGGAGTCTGAGATGAAGCAC 161 TGTAGCTCAGG hsa-mir-144-prec TGGGGCCCTGGCTGGGATATCATCATATACTGTAAGTTTGCGATGAGACACT 162 ACAGTATAGATGATGTACTAGTCCGGGCACCCCC hsa-mir-144-prec GGCTGGGATATCATCATATACTGTAAGTTTGCGATGAGACACTACAGTATAG 163 ATGATGTACTAGTC hsa-mir-145-prec CACCTTGTCCTCACGGTCCAGTTTTCCCAGGAATCCCTTAGATGCTAAGATGG 164 GGATTCCTGGAAATACTGTTCTTGAGGTCATGGTT hsa-mir-145-prec CTCACGGTCCAGTTTTCCCAGGAATCCCTTAGATGCTAAGATGGGGATTCCT 165 GGAAATACTGTTCTTGAG hsa-mir-146-prec CCGATGTGTATCCTCAGCTTTGAGAACTGAATTCCATGGGTTGTGTCAGTGTC 166 AGACCTCTGAAATTCAGTTCTTCAGCTGGGATATCTCTGTCATCGT hsa-mir-146-prec AGCTTTGAGAACTGAATTCCATGGGTTGTGTCAGTGTCAGACCTGTGAAATT 167 CAGTTCTTCAGCT hsa-mir-147-prec AATCTAAAGACAACATTTCTGCACACACACCAGACTATGGAAGCCAGTGTGT 168 GGAAATGCTTCTGCTAGATT hsa-mir-148-prec GAGGCAAAGTTCTGAGACACTCCGACTCTGAGTATGATAGAAGTCAGTGCAC 169 TACAGAACTTTGTCTC hsa-mir-149-prec GCCGGCGCCCGAGCTCTGGCTCCGTGTCTTCACTCCCGTGCTTGTCCGAGGA 170 GGGAGGGAGGGACGGGGGCTGTGCTGGGGCAGCTGGA hsa-mir-149-prec GCTCTGGCTCCGTGTCTTCACTCCCGTGCTTGTCCGAGGAGGGAGGGAGGGA 171 C hsa-mir-150-prec CTCCCCATGGCCCTGTCTCCCAACCCTTGTACCAGTGCTGGGCTCAGACCCTG 172 GTACAGGCCTGGGGGACAGGGACCTGGGGAC hsa-mir-150-prec CCCTGTCTCCCAACCCTTGTACCAGTGCTGGGCTCAGACCCTGGTACAGGCC 173 TGGGGGACAGGG hsa-mir-151-prec CCTGCCCTCGAGGAGCTCACAGTCTAGTATGTCTCATCCCCTACTAGACTGA 174 AGCTCCTTGAGGACAGG hsa-mir-152-prec TGTCCCCCCCGGCCCAGGTTCTGTGATACACTCCGACTCGGGCTCTGGAGCA 175 GTCAGTGCATGACAGAACTTGGGCCCGGAAGGACC hsa-mir-152-prec GGCCCAGGTTCTGTGATACACTCCGACTCGGGCTCTGGAGCAGTCAGTGCAT 176 GACAGAACTTGGGCCCCGG hsa-mir-153-1- CTCACAGCTGCCAGTGTCATTTTTGTGATCTGCAGCTAGTATTCTCACTCCAG 177 prec TTGCATAGTCACAAAAGTGATCATTGGCAGGTGTGGC hsa-mir-153-1- tctctctctccctcACAGCTGCCAGTGTCATTGTCACAAAAGTGATCATTGGCAGGTG 178 prec TGGCTGCTGCATG hsa-mir-153-2- AGCGGTGGCCAGTGTCATTTTTGTGATGTTGCAGCTAGTAATATGAGCCCAG 179 prec TTGCATAGTCACAAAAGTGATCATTGGAAACTGTG hsa-mir-153-2- CAGTGTCATTTTTGTGATGTTGCAGCTAGTAATATGAGCCCAGTTGCATAGTC 180 prec ACAAAAGTGATCATTG hsa-mir-154-prec GTGGTACTTGAAGATAGGTTATCCGTGTTGCCTTCGCTTTATTTGTGACGAAT 181 CATACACGGTTGACCTATTTTTCAGTACCAA hsa-mir-154-prec GAAGATAGGTTATCCGTGTTGCCTTCGCTTTATTTGTGACGAATCATACACGG 182 TTGACCTATTTTT hsa-mir-155-prec CTGTTAATGCTAATCGTGATAGGGGTTTTTGCCTCCAACTGACTCCTACATAT 183 TAGCATTAACAG hsa-mir-16-2- CAATGTCAGCAGTGCCTTAGCAGCACGTAAATATTGGCGTTAAGATTCTAAA 184 prec ATTATCTCCAGTATTAACTGTGCTGCTGAAGTAAGGTTGACCATACTCTACA GTTG hsa-mir-181a- AGAAGGGCTATCAGGCCAGCCTTCAGAGGACTCCAAGGAACATTCAACGCT 185 prec GTCGGTGAGTTTGGGATTTGAAAAAACCACTGACCGTTGACTGTACCTTGGG GTCCTTA hsa-mir-181b- TGAGTTTTGAGGTTGCTTCAGTGAACATTCAACGCTGTCGGTGAGTTTGGAA 186 prec TTAAAATCAAAACCATCGACCGTTGATTGTACCCTATGGCTAACCATCATCT ACTCCA hsa-mir-181c- CGGAAAATTTGCCAAGGGTTTGGGGGAACATTCAACCTGTCGGTGAGTTTGG 187 prec GCAGCTCAGGCAAACCATCGACCGTTGAGTGGACCCTGAGGCCTGGAATTGC CATCCT hsa-mir-182-as- GAGCTGCTTGCCTCCCCCCGTTTTTGGCAATGGTAGAACTCACACTGGTGAG 188 prec GTAACAGGATCCGGTGGTTCTAGACTTGCCAACTATGGGGCGAGGACTCAGC CGGCAC hsa-mir-182-prec TTTTTGGCAATGGTAGAACTCACACTGGTGAGGTAACAGGATCCGGTGGTTC 189 TAGACTTGCCAACTATGG hsa-mir-183-prec CCGCAGAGTGTGACTCCTGTTCTGTGTATGGCACTGGTAGAATTCACTGTGA 190 ACAGTCTCAGTCAGTGAATTACCGAAGGGCCATAAACAGAGCAGAGACAGA TCCACGA hsa-mir-184-prec CCAGTCACGTCCCCTTATCACTTTTCCAGCCCAGCTTTGTGACTGTAAGTGTT 191 GGACGGAGAACTGATAAGGGTAGGTGATTGA hsa-mir-184-prec CCTTATCACTTTTCCAGCCCAGCTTTGTGACTGTAAGTGTTGGACGGAGAACT 192 GATAAGGGTAGG hsa-mir-185-prec AGGGGGCGAGGGATTGGAGAGAAAGGCAGTTCCTGATGGTCCCCTCCCCAG 193 GGGCTGGCTTTCCTCTGGTCCTTCCCTCCCA hsa-mir-185-prec AGGGATTGGAGAGAAAGGCAGTTCCTGATGGTCCCCTCCCCAGGGGCTGGCT 194 TTCCTCTGGTCCTT hsa-mir-186-prec TGCTTGTAACTTTCCAAAGAATTCTCCTTTTGGGCTTTCTGGTTTTATTTTAAG 195 CCCAAAGGTGAATTTTTTGGGAAGTTTGAGCT hsa-mir-186-prec ACTTTCCAAAGAATTCTCCTTTTGGGCTTTCTGGTTTTATTTTAAGCCCAAAG 196 GTGAATTTTTTGGGAAGT hsa-mir-187-prec GGTCGGGCTCACCATGACACAGTGTGAGACTCGGGCTACAACACAGGACCC 197 GGGGCGCTGCTCTGACCCCTCGTGTCTTGTGTTGCAGCCGGAGGGACGCAGG TCCGCA hsa-mir-188-prec TGCTCCCTCTCTCACATCCCTTGCATGGTGGAGGGTGAGCTTTCTGAAAACCC 198 CTCCCACATGCAGGGTTTGCAGGATGGCGAGCC hsa-mir-188-prec TCTCACATCCCTTGCATGGTGGAGGGTGAGCTTTCTGAAAACCCCTCCCACA 199 TGCAGGGTTTGCAGGA hsa-mir-189-prec CTGTCGATTGGACCCGCCCTCCGGTGCCTACTGAGCTGATATCAGTTCTCATT 200 TTACACACTGGCTCAGTTCAGCAGGAACAGGAGTCGAGCCCTTGAGCAA hsa-mir-189-prec CTCCGGTGCCTACTGAGCTGATATCAGTTCTCATTTTACACACTGGCTCAGTT 201 CAGCAGGAACAGGAG hsa-mir-190-prec TGCAGGCCTCTGTGTGATATGTTTGATATATTAGGTTGTTATTTAATCCAACT 202 ATATATCAAACATATTCCTACAGTGTCTTGCC hsa-mir-190-prec CTGTGTGATATGTTTGATATATTAGGTTGTTATTTAATCCAACTATATATCAA 203 ACATATTCCTACAG hsa-mir-191-prec CGGCTGGACAGCGGGCAACGGAATCCCAAAAGCAGCTGTTGTCTCCAGAGC 204 ATTCCAGCTGCGCTTGGATTTCGTCCCCTGCTCTCCTGCCT hsa-mir-191-prec AGCGGGCAACGGAATCCCAAAAGCAGCTGTTGTCTCCAGAGCATTCCAGCTG 205 CGCTTGGATTTCGTCCCCTGCT hsa-mir-192-2/3 CCGAGACCGAGTGCACAGGGCTCTGACCTATGAATTGACAGCCAGTGCTCTC 206 GTCTCCCCTCTGGCTGCCAATTCCATAGGTCACAGGTATGTTCGCCTCAATGC CAG hsa-mir-192-prec GCCGAGACCGAGTGCACAGGGCTCTGACCTATGAATTGACAGCCAGTGCTCT 207 CGTCTCCCCTCTGGCTGCCAATTCCATAGGTCACAGGTATGTTCGCCTCAATG CCAGC hsa-mir-193-prec CGAGGATGGGAGCTGAGGGCTGGGTCTTTGCGGGCGAGATGAGGGTGTCGG 208 ATCAACTGGCCTACAAAGTCCCAGTTCTCGGCCCCCG hsa-mir-193-prec GCTGGGTCTTTGCGGGCGAGATGAGGGTGTCGGATCAACTGGCCTACAAAGT 209 CCCAGT hsa-mir-194-prec ATGGTGTTATCAAGTGTAACAGCAACTCCATGTGGACTGTGTACCAATTTCC 210 AGTGGAGATGCTGTTACTTTTGATGGTTACCAA hsa-mir-194-prec GTGTAACAGCAACTCCATGTGGACTGTGTACCAATTTCCAGTGGAGATGCTG 211 TTACTTTTGAT hsa-mir-195-prec AGCTTCCCTGGCTCTAGCAGCACAGAAATATTGGCACAGGGAAGCGAGTCTG 212 CCAATATTGGCTGTGCTGCTCCAGGCAGGGTGGTG hsa-mir-195-prec TAGCAGCACAGAAATATTGGCACAGGGAAGCGAGTCTGCCAATATTGGCTG 213 TGCTGCT hsa-mir-196-1- CTAGAGCTTGAATTGGAACTGCTGAGTGAATTAGGTAGTTTCATGTTGTTGG 214 prec GCCTGGGTTTCTGAACACAACAACATTAAACCACCCGATTCACGGCAGTTAC TGCTCC hsa-mir-196-1- GTGAATTAGGTAGTTTCATGTTGTTGGGCCTGGGTTTCTGAACACAACAACA 215 prec TTAAACCACCCGATTCAC hsa-mir-196-2- TGCTCGCTCAGCTGATCTGTGGCTTAGGTAGTTTCATGTTGTTGGGATTGAGT 216 prec TTTGAACTCGGCAACAAGAAACTGCCTGAGTTACATCAGTCGGTTTTCGTCG AGGGC hsa-mir-196-prec GTGAATTAGGTAGTTTCATGTTGTTGGGCCTGGGTTTCTGAACACAACAACA 217 TTAAACCACCCGATTCAC hsa-mir-197-prec GGCTGTGCCGGGTAGAGAGGGCAGTGGGAGGTAAGAGCTCTTCACCCTTCA 218 CCACCTTCTCCACCCAGCATGGCC hsa-mir-198-prec TCATTGGTCCAGAGGGGAGATAGGTTCCTGTGATTTTTCCTTCTTCTCTATAG 219 AATAAATGA hsa-mir-199a-1- GCCAACCCAGTGTTCAGACTACCTGTTCAGGAGGCTCTCAATGTGTACAGTA 220 prec GTCTGCACATTGGTTAGGC hsa-mir-199a-2- AGGAAGCTTCTGGAGATCCTGCTCCGTCGCCCCAGTGTTCAGACTACCTGTT 221 prec CAGGACAATGCCGTTGTACAGTAGTCTGCACATTGGTTAGACTGGGCAAGGG AGAGCA hsa-mir-199b- CCAGAGGACACCTCCACTCCGTCTACCCAGTGTTTAGACTATCTGTTCAGGA 222 prec CTCCCAAATTGTACAGTAGTCTGCACATTGGTTAGGCTGGGCTGGGTTAGAC CCTCGG hsa-mir-199s- GCCAACCCAGTGTTCAGACTACCTGTTCAGGAGGCTCTCAATGTGTACAGTA 223 prec GTCTGCACATTGGTTAGGC hsa-mir-200a- GCCGTGGCCATCTTACTGGGCAGCATTGGATGGAGTCAGGTCTCTAATACTG 224 prec CCTGGTAATGATGACGGC hsa-mir-200b- CCAGCTCGGGCAGCCGTGGCCATCTTACTGGGCAGCATTGGATGGAGTCAGG 225 prec TCTCTAATACTGCCTGGTAATGATGACGGCGGAGCCCTGCACG hsa-mir-202-prec GTTCCTTTTTCCTATGCATATACTTCTTTGAGGATCTGGCCTAAAGAGGTATA 226 GGGCATGGGAAGATGGAGC hsa-mir-203-prec GTGTTGGGGACTCGCGCGCTGGGTCCAGTGGTTCTTAACAGTTCAACAGTTC 227 TGTAGCGCAATTGTGAAATGTTTAGGACCACTAGACCCGGCGGGCGCGGCG
ACAGCGA hsa-mir-204-prec GGCTACAGTCTTTCTTCATGTGACTCGTGGACTTCCCTTTGTCATCCTATGCC 228 TGAGAATATATGAAGGAGGCTGGGAAGGCAAAGGGACGTTCAATTGTCATC ACTGGC hsa-mir-205-prec AAAGATCCTCAGACAATCCATGTGCTTCTCTTGTCCTTCATTCCACCGGAGTC 229 TGTCTCATACCCAACCAGATTTCAGTGGAGTGAAGTTCAGGAGGCATGGAGC TGACA hsa-mir-206-prec TGCTTCCCGAGGCCACATGCTTCTTTATATCCCCATATGGATTACTTTGCTAT 230 GGAATGTAAGGAAGTGTGTGGTTTCGGCAAGTG hsa-mir-206-prec AGGCCACATGCTTCTTTATATCCCCATATGGATTACTTTGCTATGGAATGTAA 231 GGAAGTGTGTGGTTTT hsa-mir-208-prec TGACGGGCGAGCTTTTGGCCCGGGTTATACCTGATGCTCACGTATAAGACGA 232 GCAAAAAGCTTGTTGGTCA hsa-mir-210-prec ACCCGGCAGTGCCTCCAGGCGCAGGGCAGCCCCTGCCCACCGCACACTGCGC 233 TGCCCCAGACCCACTGTGCGTGTGACAGCGGCTGATCTGTGCCTGGGCAGCG CGACCC hsa-mir-211-prec TCACCTGGCCATGTGACTTGTGGGCTTCCCTTTGTCATCCTTCGCCTAGGGCT 234 CTGAGCAGGGCAGGGACAGCAAAGGGGTGCTCAGTTGTCACTTCCCACAGC ACGGAG hsa-mir-212-prec CGGGGCACCCCGCCCGGACAGCGCGCCGGCACCTTGGCTCTAGACTGCTTAC 235 TGCCCGGGCCGCCCTCAGTAACAGTCTCCAGTCACGGCCACCGACGCCTGGC CCCGCC hsa-mir-213-prec CCTGTGCAGAGATTATTTTTTAAAAGGTCACAATCAACATTCATTGCTGTCGG 236 TGGGTTGAACTGTGTGGACAAGCTCACTGAACAATGAATGCAACTGTGGCCC CGCTT hsa-mir-213- GAGTTTTGAGGTTGCTTCAGTGAACATTCAACGCTGTCGGTGAGTTTGGAAT 237 prec-LIM TAAAATCAAAACCATCGACCGTTGATTGTACCCTATGGCTAACCATCATCTA CTCC hsa-mir-214-prec GGCCTGGCTGGACAGAGTTGTCATGTGTCTGCCTGTCTACACTTGCTGTGCA 238 GAACATCCGCTCACCTGTACAGCAGGCACAGACAGGCAGTCACATGACAAC CCAGCCT hsa-mir-215-prec ATCATTCAGAAATGGTATACAGGAAAATGACCTATGAATTGACAGACAATAT 239 AGCTGAGTTTGTCTGTCATTTCTTTAGGCCAATATTCTGTATGACTGTGCTAC TTCAA hsa-mir-216-prec GATGGCTGTGAGTTGGCTTAATCTCAGCTGGCAACTGTGAGATGTTCATACA 240 ATCCCTCACAGTGGTCTCTGGGATTATGCTAAACAGAGCAATTTCCTAGCCC TCACGA hsa-mir-217-prec AGTATAATTATTACATAGTTTTTGATGTCGCAGATACTGCATCAGGAACTGA 241 TTGGATAAGAATCAGTCACCATCAGTTCCTAATGCATTGCCTTCAGCATCTA AACAAG hsa-mir-218-1- GTGATAATGTAGCGAGATTTTCTGTTGTGCTTGATCTAACCATGTGGTTGCGA 242 prec GGTATGAGTAAAACATGGTTCCGTCAAGCACCATGGAACGTCACGCAGCTTT CTACA hsa-mir-218-2- GACCAGTCGCTGCGGGGCTTTCCTTTGTGCTTGATCTAACCATGTGGTGGAA 243 prec CGATGGAAACGGAACATGGTTCTGTCAAGCACCGCGGAAAGCACCGTGCTC TCCTGCA hsa-mir-219-prec CCGCCCCGGGCCGCGGCTCCTGATTGTCCAAACGCAATTCTCGAGTCTATGG 244 CTCCGGCCGAGAGTTGAGTCTGGACGTCCCGAGCCGCCGCCCCCAAACCTCG AGCGGG hsa-mir-220-prec GACAGTGTGGCATTGTAGGGCTCCACACCGTATCTGACACTTTGGGCGAGGG 245 CACCATGCTGAAGGTGTTCATGATGCGGTCTGGGAACTCCTCACGGATCTTA CTGATG hsa-mir-221-prec TGAACATCCAGGTCTGGGGCATGAACCTGGCATACAATGTAGATTTCTGTGT 246 TCGTTAGGCAACAGCTACATTGTCTGCTGGGTTTCAGGCTACCTGGAAACAT GTTCTC hsa-mir-222-prec GCTGCTGGAAGGTGTAGGTACCCTCAATGGCTCAGTAGCCAGTGTAGATCCT 247 GTCTTTCGTAATCAGCAGCTACATCTGGCTACTGGGTCTCTGATGGCATCTTC TAGCT hsa-mir-223-prec CCTGGCCTCCTGCAGTGCCACGCTCCGTGTATTTGACAAGCTGAGTTGGACA 248 CTCCATGTGGTAGAGTGTCAGTTTGTCAAATACCCCAAGTGCGGCACATGCT TACCAG hsa-mir-224-prec GGGCTTTCAAGTCACTAGTGGTTCCGTTTAGTAGATGATTGTGCATTGTTTCA 249 AAATGGTGCCCTAGTGACTACAAAGCCC hsA-mir-29b- CTTCTGGAAGCTGGTTTCACATGGTGGCTTAGATTTTTCCATCTTTGTATCTA 250 1 = 102-prec1 GCACCATTTGAAATCAGTGTTTTAGGAG hsA-mir-29b- CTTCAGGAAGCTGGTTTCATATGGTGGTTTAGATTTAAATAGTGATTGTCTAG 251 2 = 102prec7.1 = CACCATTTGAAATCAGTGTTCTTGGGGG 7.2 hsA-mir-29b- CTTCAGGAAGCTGGTTTCATATGGTGGTTTAGATTTAAATAGTGATTGTCTAG 252 3 = 102prec7.1 = CACCATTTGAAATCAGTGTTCTTGGGGG 7.2 hsa-mir- GTGAGCGACTGTAAACATCCTCGACTGGAAGCTGTGAAGCCACAGATGGGC 253 30* = mir-097- TTTCAGTCGGATGTTTGCAGCTGCCTACT prec-6 mir-033b ACCAAGTTTCAGTTCATGTAAACATCCTACACTCAGCTGTAATACATGGATT 254 GGCTGGGAGGTGGATGTTTACTTCAGCTGACTTGGA mir-101- TGCCCTGGCTCAGTTATCACAGTGCTGATGCTGTCTATTCTAAAGGTACAGTA 255 precursor-9 = mir- CTGTGATAACTGAAGGATGGCA 101-3 mir-108-1-small ACACTGCAAGAACAATAAGGATTTTTAGGGGCATTATGACTGAGTCAGAAA 256 ACACAGCTGCCCCTGAAAGTCCCTCATTTTTCTTGCTGT mir-108-2-small ACTGCAAGAGCAATAAGGATTTTTAGGGGCATTATGATAGTGGAATGGAAA 257 CACATCTGCCCCCAAAAGTCCCTCATTTT mir-123-prec = CGCTGGCGACGGGACATTATTACTTTTGGTACGCGCTGTGACACTTCAAACT 258 mir-126-prec CGTACCGTGAGTAATAATGCGCCGTCCACGGCA mir-123-prec = ACATTATTACTTTTGGTACGCGCTGTGACACTTCAAACTCGTACCGTGAGTAA 259 mir-126-prec TAATGCGC mir-129-1-prec TGGATCTTTTTGCGGTCTGGGCTTGCTGTTCCTCTCAACAGTAGTCAGGAAGC 260 CCTTACCCCAAAAAGTATCTA mir-129-small- TGCCCTTCGCGAATCTTTTTGCGGTCTGGGCTTGCTGTACATAACTCAATAGC 261 2 = 129b? CGGAAGCCCTTACCCCAAAAAGCATTTGCGGAGGGCG mir-133b-small GCCCCCTGCTCTGGCTGGTCAAACGGAACCAAGTCCGTCTTCCTGAGAGGTT 262 TGGTCCCCTTCAACCAGCTACAGCAGGG mir-135-small-2 AGATAAATTCACTCTAGTGCTTTATGGCTTTTTATTCCTATGTGATAGTAATA 263 AAGTCTCATGTAGGGATGGAAGCCATGAAATACATTGTGAAAAATCA mir-148b-small AAGCACGATTAGCATTTGAGGTGAAGTTCTGTTATACACTCAGGCTGTGGCT 264 CTCTGAAAGTCAGTGCAT mir-151-prec CCTGTCCTCAAGGAGCTTCAGTCTAGTAGGGGATGAGACATACTAGACTGTG 265 AGCTCCTCGAGGGCAGG mir-155- CTGTTAATGCTAATCGTGATAGGGGTTTTTGCCTCCAACTGACTCCTACATAT 266 prec(BIC) TAGCATTAACAG mir-156 = mir- CCTAACACTGTCTGGTAAAGATGGCTCCCGGGTGGGTTCTCTCGGCAGTAAC 267 157 = overlap CTTCAGGGAGCCCTGAAGACCATGGAGGAC mir-141 mir-158-small = GCCGAGACCGAGTGCACAGGGCT AGTGCTCT 268 mir-192 CGTCTCCCCTCTGGCTGCCAATTCCATAGGTCACAGGTATGTTCGCCTCAATG CCAGC mir-159-1-small TCCCGCCCCCTGTAACAGCAACTCCATGTGGAAGTGCCCACTGGTTCCAGTG 269 GGGCTGCTGTTATCTGGGGCGAGGGCCA mir-161-small AAAGCTGGGTTGAGAGGGCGAAAAAGGATGAGGTGACTGGTCTGGGCTACG 270 CTATGCTGCGGCGCTCGGG mir-163-1b- CATTGGCCTCCTAAGCCAGGGATTGTGGGTTCGAGTCCCACCCGGGGTAAAG 271 small AAAGGCCGAATT mir-163-3-small CCTAAGCCAGGGATTGTGGGTTCGAGTCCCACCTGGGGTAGAGGTGAAAGTT 272 CCTTTTACGGAATTTTTT mir-175- GGGCTTTCAAGTCACTAGTGGTTCCGTTTAGTAGATGATTGTGCATTGTTTCA 273 small = mir-224 AAATGGTGCCCTAGTGACTACAAAGCCC mir-177- small ACGCAAGTGTCCTAAGGTGAGCTCAGGGAGCACAGAAACCTCCAGTGGAAC 274 AGAAGGGCAAAAGCTCATT mir-180- small CATGTGTCACTTTCAGGTGGAGTTTCAAGAGTCCCTTCCTGGTTCACCGTCTC 275 CTTTGCTCTTCCACAAC mir-187-prec GGTCGGGCTCACCATGACACAGTGTGAGACTCGGGCTACAACACAGGACCC 276 GGGGCGCTGCTCTGACCCCTCGTGTCTTGTGTTGCAGCCGGAGGGACGCAGG TCCGCA mir-188-prec TGCTCCCTCTCTCACATCCCTTGCATGGTGGAGGGTGAGCTTTCTGAAAACCC 277 CTCCCACATGCAGGGTTTGCAGGATGGCGAGCC mir-190-prec TGCAGGCCTCTGTGTGATATGTTTGATATATTAGGTTGTTATTTAATCCAACT 278 ATATATCAAACATATTCCTACAGTGTCTTGCC mir-197-2 GTGCATGTGTATGTATGTGTGCATGTGCATGTGTATGTGTATGAGTGCATGC 279 GTGTGTGC mir-197-prec GGCTGTGCCGGGTAGAGAGGGCAGTGGGAGGTAAGAGCTCTTCACCCTTCA 280 CCACCTTCTCCACCCAGCATGGCC mir-202-prec GTTCCTTTTTCCTATGCATATACTTCTTTGAGGATCTGGCCTAAAGAGGTATA 281 GGGCATGGGAAGATGGAGC mir-294-1 CAATCTTCCTTTATCATGGTATTGATTTTTCAGTGCTTCCCTTTTGTGTGAGAG 282 (chr16) AAGATA mir-hes1 ATGGAGCTGCTCACCCTGTGGGCCTCAAATGTGGAGGAACTATTCTGATGTC 283 CAAGTGGAAAGTGCTGCGACATTTGAGCGTCACCGGTGACGCCCATATCA mir-hes2 GCATCCCCTCAGCCTGTGGCACTCAAACTGTGGGGGCACTTTCTGCTCTCTGG 284 TGAAAGTGCCGCCATCTTTTGAGTGTTACCGCTTGAGAAGACTCAACC mir-hes3 CGAGGAGCTCATACTGGGATACTCAAAATGGGGGCGCTTTCCTTTTTGTCTG 285 TTACTGGGAAGTGCTTCGATTTTGGGGTGTCCCTGTTTGAGTAGGGCATC hsa-mir-29b-1 CTTCAGGAAGCTGGTTTCATATGGTGGTTTAGATTTAAATAGTGATTGTCTAG 286 CACCATTTGAAATCAGTGTTCTTGGGGG *An underlined sequence within a precursor sequence represents a processed miR transcript. All sequences are human.
[0039] The level of at least one miR gene product can be measured in cells of a biological sample obtained from the subject. For example, a tissue sample can be removed from a subject suspected of having breast cancer associated with by conventional biopsy techniques. In another example, a blood sample can be removed from the subject, and white blood cells can be isolated for DNA extraction by standard techniques. The blood or tissue sample is preferably obtained from the subject prior to initiation of radiotherapy, chemotherapy or other therapeutic treatment. A corresponding control tissue or blood sample can be obtained from unaffected tissues of the subject, from a normal human individual or population of normal individuals, or from cultured cells corresponding to the majority of cells in the subject's sample. The control tissue or blood sample is then processed along with the sample from the subject, so that the levels of miR gene product produced from a given miR gene in cells from the subject's sample can be compared to the corresponding miR gene product levels from cells of the control sample.
[0040] An alteration (i.e., an increase or decrease) in the level of a miR gene product in the sample obtained from the subject, relative to the level of a corresponding miR gene product in a control sample, is indicative of the presence of breast cancer in the subject. In one embodiment, the level of the at least one miR gene product in the test sample is greater than the level of the corresponding miR gene product in the control sample (i.e., expression of the miR gene product is "up-regulated"). As used herein, expression of a miR gene product is "up-regulated" when the amount of miR gene product in a cell or tissue sample from a subject is greater than the amount the same gene product in a control cell or tissue sample. In another embodiment, the level of the at least one miR gene product in the test sample is less than the level of the corresponding miR gene product in the control sample (i.e., expression of the miR gene product is "down-regulated"). As used herein, expression of a miR gene is "down-regulated" when the amount of miR gene product produced from that gene in a cell or tissue sample from a subject is less than the amount produced from the same gene in a control cell or tissue sample. The relative miR gene expression in the control and normal samples can be determined with respect to one or more RNA expression standards. The standards can comprise, for example, a zero miR gene expression level, the miR gene expression level in a standard cell line, or the average level of miR gene expression previously obtained for a population of normal human controls.
[0041] The level of a miR gene product in a sample can be measured using any technique that is suitable for detecting RNA expression levels in a biological sample. Suitable techniques for determining RNA expression levels in cells from a biological sample (for example, Northern blot analysis, RT-PCR, in situ hybridization) are well known to those of skill in the art. In a particular embodiment, the level of at least one miR gene product is detected using Northern blot analysis. For example, total cellular RNA can be purified from cells by homogenization in the presence of nucleic acid extraction buffer, followed by centrifugation. Nucleic acids are precipitated, and DNA is removed by treatment with DNase and precipitation. The RNA molecules are then separated by gel electrophoresis on agarose gels according to standard techniques, and transferred to nitrocellulose filters. The RNA is then immobilized on the filters by heating. Detection and quantification of specific RNA is accomplished using appropriately labeled DNA or RNA probes complementary to the RNA in question. See, for example, Molecular Cloning: A Laboratory Manual, J. Sambrook et al., eds., 2nd edition, Cold Spring Harbor Laboratory Press, 1989, Chapter 7, the entire disclosure of which is incorporated by reference.
[0042] Suitable probes for Northern blot hybridization of a given miR gene product can be produced from the nucleic acid sequences provided in Table 1. Methods for preparation of labeled DNA and RNA probes, and the conditions for hybridization thereof to target nucleotide sequences, are described in Molecular Cloning: A Laboratory Manual, J. Sambrook et al., eds., 2nd edition, Cold Spring Harbor Laboratory Press, 1989, Chapters 10 and 11, the disclosures of which are incorporated herein by reference.
[0043] For example, the nucleic acid probe can be labeled with, for example, a radionuclide, such as 3H, 32P, 33P, 14C, or 35S; a heavy metal; or a ligand capable of functioning as a specific binding pair member for a labeled ligand (for example, biotin, avidin or an antibody), a fluorescent molecule, a chemiluminescent molecule, an enzyme or the like.
[0044] Probes can be labeled to high specific activity by either the nick translation method of Rigby et al. (1977), J. Mol. Biol. 113:237-251 or by the random priming method of Fienberg et al. (1983), Anal. Biochem. 132:6-13, the entire disclosures of which are incorporated herein by reference. The latter is the method of choice for synthesizing 32P-labeled probes of high specific activity from single-stranded DNA or from RNA templates. For example, by replacing preexisting nucleotides with highly radioactive nucleotides according to the nick translation method, it is possible to prepare 32P-labeled nucleic acid probes with a specific activity well in excess of 108 cpm/microgram. Autoradiographic detection of hybridization can then be performed by exposing hybridized filters to photographic film. Densitometric scanning of the photographic films exposed by the hybridized filters provides an accurate measurement of miR gene transcript levels. Using another approach, miR gene transcript levels can be quantified by computerized imaging systems, such the Molecular Dynamics 400-B 2D Phosphorimager available from Amersham Biosciences, Piscataway, N.J.
[0045] Where radionuclide labeling of DNA or RNA probes is not practical, the random-primer method can be used to incorporate an analogue, for example, the dTTP analogue 5-(N--(N-biotinyl-epsilon-aminocaproyl)-3-aminoallyl)deoxyuridine triphosphate, into the probe molecule. The biotinylated probe oligonucleotide can be detected by reaction with biotin-binding proteins, such as avidin, streptavidin, and antibodies (for example, anti-biotin antibodies) coupled to fluorescent dyes or enzymes that produce color reactions.
[0046] In addition to Northern and other RNA hybridization techniques, determining the levels of RNA transcripts can be accomplished using the technique of in situ hybridization. This technique requires fewer cells than the Northern blotting technique, and involves depositing whole cells onto a microscope cover slip and probing the nucleic acid content of the cell with a solution containing radioactive or otherwise labeled nucleic acid (for example, cDNA or RNA) probes. This technique is particularly well-suited for analyzing tissue biopsy samples from subjects. The practice of the in situ hybridization technique is described in more detail in U.S. Pat. No. 5,427,916, the entire disclosure of which is incorporated herein by reference. Suitable probes for in situ hybridization of a given miR gene product can be produced from the nucleic acid sequences provided in Table 1, as described above.
[0047] The relative number of miR gene transcripts in cells can also be determined by reverse transcription of miR gene transcripts, followed by amplification of the reverse-transcribed transcripts by polymerase chain reaction (RT-PCR). The levels of miR gene transcripts can be quantified in comparison with an internal standard, for example, the level of mRNA from a "housekeeping" gene present in the same sample. A suitable "housekeeping" gene for use as an internal standard includes, for example, myosin or glyceraldehyde-3-phosphate dehydrogenase (G3PDH). The methods for quantitative RT-PCR and variations thereof are within the skill in the art.
[0048] In some instances, it may be desirable to simultaneously determine the expression level of a plurality of different miR gene products in a sample. In other instances, it may be desirable to determine the expression level of the transcripts of all known miR genes correlated with a cancer. Assessing cancer-specific expression levels for hundreds of miR genes is time consuming and requires a large amount of total RNA (at least 20 μg for each Northern blot) and autoradiographic techniques that require radioactive isotopes.
[0049] To overcome these limitations, an oligolibrary, in microchip format (i.e., a microarray), may be constructed containing a set of probe oligodeoxynucleotides that are specific for a set of miR genes. Using such a microarray, the expression level of multiple microRNAs in a biological sample can be determined by reverse transcribing the RNAs to generate a set of target oligodeoxynucleotides, and hybridizing them to probe oligodeoxynucleotides on the microarray to generate a hybridization, or expression, profile. The hybridization profile of the test sample can then be compared to that of a control sample to determine which microRNAs have an altered expression level in breast cancer cells. As used herein, "probe oligonucleotide" or "probe oligodeoxynucleotide" refers to an oligonucleotide that is capable of hybridizing to a target oligonucleotide. "Target oligonucleotide" or "target oligodeoxynucleotide" refers to a molecule to be detected (for example, via hybridization). By "miR-specific probe oligonucleotide" or "probe oligonucleotide specific for a miR" is meant a probe oligonucleotide that has a sequence selected to hybridize to a specific miR gene product, or to a reverse transcript of the specific miR gene product.
[0050] An "expression profile" or "hybridization profile" of a particular sample is essentially a fingerprint of the state of the sample; while two states may have any particular gene similarly expressed, the evaluation of a number of genes simultaneously allows the generation of a gene expression profile that is unique to the state of the cell. That is, normal tissue may be distinguished from breast cancer tissue, and within breast cancer tissue, different prognosis states (good or poor long term survival prospects, for example) may be determined. By comparing expression profiles of breast cancer tissue in different states, information regarding which genes are important (including both up- and down-regulation of genes) in each of these states is obtained. The identification of sequences that are differentially expressed in breast cancer tissue or normal breast tissue, as well as differential expression resulting in different prognostic outcomes, allows the use of this information in a number of ways. For example, a particular treatment regime may be evaluated (for example, to determine whether a chemotherapeutic drug act to improve the long-term prognosis in a particular patient). Similarly, diagnosis may be done or confirmed by comparing patient samples with the known expression profiles. Furthermore, these gene expression profiles (or individual genes) allow screening of drug candidates that suppress the breast cancer expression profile or convert a poor prognosis profile to a better prognosis profile.
[0051] Accordingly, the invention provides methods of diagnosing whether a subject has, or is at risk for developing, breast cancer, comprising reverse transcribing RNA from a test sample obtained from the subject to provide a set of target oligo-deoxynucleotides, hybridizing the target oligo-deoxynucleotides to a microarray comprising miRNA-specific probe oligonucleotides to provide a hybridization profile for the test sample, and comparing the test sample hybridization profile to a hybridization profile generated from a control sample, wherein an alteration in the signal of at least one miRNA is indicative of the subject either having, or being at risk for developing, breast cancer. In one embodiment, the microarray comprises miRNA-specific probe oligonucleotides for a substantial portion of the human miRNome. In a particular embodiment, the microarray comprises miRNA-specific probe oligo-nucleotides for one or more miRNAs selected from the group consisting of miR-125b, miR-145, miR-21, miR-155, miR-10b, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, miR-213, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, let-7i (let-7d-v2), miR-101-1, miR-122a, miR-128b, miR-136, miR-143, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210 and combinations thereof. In a further embodiment, the at least one miR gene product is selected from the group consisting of miR-125b, miR-145, miR-21, miR-155, miR-10b and combinations thereof.
[0052] The microarray can be prepared from gene-specific oligonucleotide probes generated from known miRNA sequences. The array may contain two different oligonucleotide probes for each miRNA, one containing the active, mature sequence and the other being specific for the precursor of the miRNA. The array may also contain controls, such as one or more mouse sequences differing from human orthologs by only a few bases, which can serve as controls for hybridization stringency conditions. tRNAs from both species may also be printed on the microchip, providing an internal, relatively stable, positive control for specific hybridization. One or more appropriate controls for non-specific hybridization may also be included on the microchip. For this purpose, sequences are selected based upon the absence of any homology with any known miRNAs.
[0053] The microarray may be fabricated using techniques known in the art. For example, probe oligonucleotides of an appropriate length, for example, 40 nucleotides, are 5'-amine modified at position C6 and printed using commercially available microarray systems, for example, the GeneMachine OmniGrid® 100 Microarrayer and Amersham CodeLink® activated slides. Labeled cDNA oligomer corresponding to the target RNAs is prepared by reverse transcribing the target RNA with labeled primer. Following first strand synthesis, the RNA/DNA hybrids are denatured to degrade the RNA templates. The labeled target cDNAs thus prepared are then hybridized to the microarray chip under hybridizing conditions, for example, 6×SSPE/30% formamide at 25° C. for 18 hours, followed by washing in 0.75×TNT at 37° C. for 40 minutes. At positions on the array where the immobilized probe DNA recognizes a complementary target cDNA in the sample, hybridization occurs. The labeled target cDNA marks the exact position on the array where binding occurs, allowing automatic detection and quantification. The output consists of a list of hybridization events, indicating the relative abundance of specific cDNA sequences, and therefore the relative abundance of the corresponding complementary miRs, in the patient sample. According to one embodiment, the labeled cDNA oligomer is a biotin-labeled cDNA, prepared from a biotin-labeled primer. The microarray is then processed by direct detection of the biotin-containing transcripts using, for example, Streptavidin-Alexa647 conjugate, and scanned utilizing conventional scanning methods. Image intensities of each spot on the array are proportional to the abundance of the corresponding miR in the patient sample.
[0054] The use of the array has several advantages for miRNA expression detection. First, the global expression of several hundred genes can be identified in the same sample at one time point. Second, through careful design of the oligonucleotide probes, expression of both mature and precursor molecules can be identified. Third, in comparison with Northern blot analysis, the chip requires a small amount of RNA, and provides reproducible results using 2.5 μg of total RNA. The relatively limited number of miRNAs (a few hundred per species) allows the construction of a common microarray for several species, with distinct oligonucleotide probes for each. Such a tool would allow for analysis of trans-species expression for each known miR under various conditions.
[0055] In addition to use for quantitative expression level assays of specific miRs, a microchip containing miRNA-specific probe oligonucleotides corresponding to a substantial portion of the miRNome, preferably the entire miRNome, may be employed to carry out miR gene expression profiling, for analysis of miR expression patterns. Distinct miR signatures can be associated with established disease markers, or directly with a disease state.
[0056] According to the expression profiling methods described herein, total RNA from a sample from a subject suspected of having a cancer (such as breast cancer) is quantitatively reverse transcribed to provide a set of labeled target oligodeoxynucleotides complementary to the RNA in the sample. The target oligodeoxynucleotides are then hybridized to a microarray comprising miRNA-specific probe oligonucleotides to provide a hybridization profile for the sample. The result is a hybridization profile for the sample representing the expression pattern of miRNA in the sample. The hybridization profile comprises the signal from the binding of the target oligodeoxynucleotides from the sample to the miRNA-specific probe oligonucleotides in the microarray. The profile may be recorded as the presence or absence of binding (signal vs. zero signal). More preferably, the profile recorded includes the intensity of the signal from each hybridization. The profile is compared to the hybridization profile generated from a normal, noncancerous, control sample. An alteration in the signal is indicative of the presence of the cancer in the subject.
[0057] Other techniques for measuring miR gene expression are also within the skill in the art, and include various techniques for measuring rates of RNA transcription and degradation.
[0058] The invention also provides methods of diagnosing a breast cancer associated with one or more prognostic markers, comprising measuring the level of at least one miR gene product in a breast cancer test sample from a subject and comparing the level of the at least one miR gene product in the breast cancer test sample to the level of a corresponding miR gene product in a control sample. An alteration (for example, an increase, a decrease) in the signal of at least one miRNA in the test sample relative to the control sample is indicative of the subject either having, or being at risk for developing, breast cancer associated with the one or more prognostic markers.
[0059] The breast cancer can be associated with one or more prognostic markers or features, including, a marker associated with an adverse (negative) prognosis, or a marker associated with a good (positive) prognosis. In certain embodiments, the breast cancer that is diagnosed using the methods described herein is associated with one or more adverse prognostic features selected from the group consisting of estrogen receptor expression, progesterone receptor expression, positive lymph node metastasis, high proliferative index, detectable p53 expression, advanced tumor stage, and high vascular invasion. Particular microRNAs whose expression is altered in breast cancer cells associated with each of these prognostic markers are described herein (see, for example, Example 3 and FIG. 4). In one embodiment, the level of the at least one miR gene product is measured by reverse transcribing RNA from a test sample obtained from the subject to provide a set of target oligodeoxynucleotides, hybridizing the target oligodeoxynucleotides to a microarray that comprises miRNA-specific probe oligonucleotides to provide a hybridization profile for the test sample, and comparing the test sample hybridization profile to a hybridization profile generated from a control sample.
[0060] Without wishing to be bound by any one theory, it is believed that alterations in the level of one or more miR gene products in cells can result in the deregulation of one or more intended targets for these miRs, which can lead to the formation of breast cancer. Therefore, altering the level of the miR gene product (for example, by decreasing the level of a miR that is up-regulated in breast cancer cells and/or by increasing the level of a miR that is down-regulated in cancer cells) may successfully treat the breast cancer. Examples of putative gene targets for miRNAs that are deregulated in breast cancer tissues are described herein (see, for example, Example 2 and Table 4).
[0061] Accordingly, the present invention encompasses methods of treating breast cancer in a subject, wherein at least one miR gene product is de-regulated (for example, down-regulated or up-regulated) in the cancer cells of the subject. When the at least one isolated miR gene product is down-regulated in the breast cancer cells, the method comprises administering an effective amount of the at least one isolated miR gene product, provided that the miR gene is not miR15 or miR16, such that proliferation of cancer cells in the subject is inhibited. When the at least one isolated miR gene product is up-regulated in the cancer cells, the method comprises administering to the subject an effective amount of at least one compound for inhibiting expression of the at least one miR gene, referred to herein as miR gene expression inhibition compounds, such that proliferation of breast cancer cells is inhibited.
[0062] The terms "treat", "treating" and "treatment", as used herein, refer to ameliorating symptoms associated with a disease or condition, for example, breast cancer, including preventing or delaying the onset of the disease symptoms, and/or lessening the severity or frequency of symptoms of the disease or condition. The terms "subject" and "individual" are defined herein to include animals, such as mammals, including but not limited to, primates, cows, sheep, goats, horses, dogs, cats, rabbits, guinea pigs, rats, mice or other bovine, ovine, equine, canine, feline, rodent, or murine species. In a preferred embodiment, the animal is a human.
[0063] As used herein, an "effective amount" of an isolated miR gene product is an amount sufficient to inhibit proliferation of a cancer cell in a subject suffering from breast cancer. One skilled in the art can readily determine an effective amount of an miR gene product to be administered to a given subject, by taking into account factors, such as the size and weight of the subject; the extent of disease penetration; the age, health and sex of the subject; the route of administration; and whether the administration is regional or systemic.
[0064] For example, an effective amount of an isolated miR gene product can be based on the approximate weight of a tumor mass to be treated. The approximate weight of a tumor mass can be determined by calculating the approximate volume of the mass, wherein one cubic centimeter of volume is roughly equivalent to one gram. An effective amount of the isolated miR gene product based on the weight of a tumor mass can be in the range of about 10-500 micrograms/gram of tumor mass. In certain embodiments, the tumor mass can be at least about 10 micrograms/gram of tumor mass, at least about 60 micrograms/gram of tumor mass or at least about 100 micrograms/gram of tumor mass.
[0065] An effective amount of an isolated miR gene product can also be based on the approximate or estimated body weight of a subject to be treated. Preferably, such effective amounts are administered parenterally or enterally, as described herein. For example, an effective amount of the isolated miR gene product is administered to a subject can range from about 5-3000 micrograms/kg of body weight, from about 700-1000 micrograms/kg of body weight, or greater than about 1000 micrograms/kg of body weight.
[0066] One skilled in the art can also readily determine an appropriate dosage regimen for the administration of an isolated miR gene product to a given subject. For example, a miR gene product can be administered to the subject once (for example, as a single injection or deposition). Alternatively, a miR gene product can be administered once or twice daily to a subject for a period of from about three to about twenty-eight days, more particularly from about seven to about ten days. In a particular dosage regimen, a miR gene product is administered once a day for seven days. Where a dosage regimen comprises multiple administrations, it is understood that the effective amount of the miR gene product administered to the subject can comprise the total amount of gene product administered over the entire dosage regimen.
[0067] As used herein, an "isolated" miR gene product is one which is synthesized, or altered or removed from the natural state through human intervention. For example, a synthetic miR gene product, or a miR gene product partially or completely separated from the coexisting materials of its natural state, is considered to be "isolated." An isolated miR gene product can exist in substantially-purified form, or can exist in a cell into which the miR gene product has been delivered. Thus, a miR gene product which is deliberately delivered to, or expressed in, a cell is considered an "isolated" miR gene product. A miR gene product produced inside a cell from a miR precursor molecule is also considered to be "isolated" molecule.
[0068] Isolated miR gene products can be obtained using a number of standard techniques. For example, the miR gene products can be chemically synthesized or recombinantly produced using methods known in the art. In one embodiment, miR gene products are chemically synthesized using appropriately protected ribonucleoside phosphoramidites and a conventional DNA/RNA synthesizer. Commercial suppliers of synthetic RNA molecules or synthesis reagents include, for example, Proligo (Hamburg, Germany), Dharmacon Research (Lafayette, Colo., U.S.A.), Pierce Chemical (part of Perbio Science, Rockford, Ill., U.S.A.), Glen Research (Sterling, Va., U.S.A.), ChemGenes (Ashland, Mass., U.S.A.) and Cruachem (Glasgow, UK).
[0069] Alternatively, the miR gene products can be expressed from recombinant circular or linear DNA plasmids using any suitable promoter. Suitable promoters for expressing RNA from a plasmid include, for example, the U6 or H1 RNA pol III promoter sequences, or the cytomegalovirus promoters. Selection of other suitable promoters is within the skill in the art. The recombinant plasmids of the invention can also comprise inducible or regulatable promoters for expression of the miR gene products in cancer cells.
[0070] The miR gene products that are expressed from recombinant plasmids can be isolated from cultured cell expression systems by standard techniques. The miR gene products which are expressed from recombinant plasmids can also be delivered to, and expressed directly in, the cancer cells. The use of recombinant plasmids to deliver the miR gene products to cancer cells is discussed in more detail below.
[0071] The miR gene products can be expressed from a separate recombinant plasmid, or they can be expressed from the same recombinant plasmid. In one embodiment, the miR gene products are expressed as RNA precursor molecules from a single plasmid, and the precursor molecules are processed into the functional miR gene product by a suitable processing system, including, but not limited to, processing systems extant within a cancer cell. Other suitable processing systems include, for example, the in vitro Drosophila cell lysate system (for example, as described in U.S. Published Patent Application No. 2002/0086356 to Tuschl et al., the entire disclosure of which are incorporated herein by reference) and the E. coli RNAse III system (for example, as described in U.S. Published Patent Application No. 2004/0014113 to Yang et al., the entire disclosure of which are incorporated herein by reference).
[0072] Selection of plasmids suitable for expressing the miR gene products, methods for inserting nucleic acid sequences into the plasmid to express the gene products, and methods of delivering the recombinant plasmid to the cells of interest are within the skill in the art. See, for example, Zeng et al. (2002), Molecular Cell 9:1327-1333; Tuschl (2002), Nat. Biotechnol, 20:446-448; Brummelkamp et al. (2002), Science 296:550-553; Miyagishi et al. (2002), Nat. Biotechnol. 20:497-500; Paddison et al. (2002), Genes Dev. 16:948-958; Lee et al. (2002), Nat. Biotechnol. 20:500-505; and Paul et al. (2002), Nat. Biotechnol. 20:505-508, the entire disclosures of which are incorporated herein by reference.
[0073] In one embodiment, a plasmid expressing the miR gene products comprises a sequence encoding a miR precursor RNA under the control of the CMV intermediate-early promoter. As used herein, "under the control" of a promoter means that the nucleic acid sequences encoding the miR gene product are located 3' of the promoter, so that the promoter can initiate transcription of the miR gene product coding sequences.
[0074] The miR gene products can also be expressed from recombinant viral vectors. It is contemplated that the miR gene products can be expressed from two separate recombinant viral vectors, or from the same viral vector. The RNA expressed from the recombinant viral vectors can either be isolated from cultured cell expression systems by standard techniques, or can be expressed directly in cancer cells. The use of recombinant viral vectors to deliver the miR gene products to cancer cells is discussed in more detail below.
[0075] The recombinant viral vectors of the invention comprise sequences encoding the miR gene products and any suitable promoter for expressing the RNA sequences. Suitable promoters include, for example, the U6 or H1 RNA pol III promoter sequences, or the cytomegalovirus promoters. Selection of other suitable promoters is within the skill in the art. The recombinant viral vectors of the invention can also comprise inducible or regulatable promoters for expression of the miR gene products in a cancer cell.
[0076] Any viral vector capable of accepting the coding sequences for the miR gene products can be used; for example, vectors derived from adenovirus (AV); adeno-associated virus (AAV); retroviruses (for example, lentiviruses (LV), Rhabdoviruses, murine leukemia virus); herpes virus, and the like. The tropism of the viral vectors can be modified by pseudotyping the vectors with envelope proteins or other surface antigens from other viruses, or by substituting different viral capsid proteins, as appropriate.
[0077] For example, lentiviral vectors of the invention can be pseudotyped with surface proteins from vesicular stomatitis virus (VSV), rabies, Ebola, Mokola, and the like. AAV vectors of the invention can be made to target different cells by engineering the vectors to express different capsid protein serotypes. For example, an AAV vector expressing a serotype 2 capsid on a serotype 2 genome is called AAV 2/2. This serotype 2 capsid gene in the AAV 2/2 vector can be replaced by a serotype 5 capsid gene to produce an AAV 2/5 vector. Techniques for constructing AAV vectors that express different capsid protein serotypes are within the skill in the art; see, for example, Rabinowitz, J. E., et al. (2002), J. Virol. 76:791-801, the entire disclosure of which is incorporated herein by reference.
[0078] Selection of recombinant viral vectors suitable for use in the invention, methods for inserting nucleic acid sequences for expressing RNA into the vector, methods of delivering the viral vector to the cells of interest, and recovery of the expressed RNA products are within the skill in the art. See, for example, Dornburg (1995), Gene Therap. 2:301-310; Eglitis (1988), Biotechniques 6:608-614; Miller (1990), Hum. Gene Therap. 1:5-14; and Anderson (1998), Nature 392:25-30, the entire disclosures of which are incorporated herein by reference.
[0079] Particularly suitable viral vectors are those derived from AV and AAV. A suitable AV vector for expressing the miR gene products, a method for constructing the recombinant AV vector, and a method for delivering the vector into target cells, are described in Xia et al. (2002), Nat. Biotech. 20:1006-1010, the entire disclosure of which is incorporated herein by reference. Suitable AAV vectors for expressing the miR gene products, methods for constructing the recombinant AAV vector, and methods for delivering the vectors into target cells are described in Samulski et al. (1987), J. Virol. 61:3096-3101; Fisher et al. (1996), J. Virol., 70:520-532; Samulski et al. (1989), J. Virol. 63:3822-3826; U.S. Pat. No. 5,252,479; U.S. Pat. No. 5,139,941; International Patent Application No. WO 94/13788; and International Patent Application No. WO 93/24641, the entire disclosures of which are incorporated herein by reference. In one embodiment, the miR gene products are expressed from a single recombinant AAV vector comprising the CMV intermediate early promoter.
[0080] In a certain embodiment, a recombinant AAV viral vector of the invention comprises a nucleic acid sequence encoding a miR precursor RNA in operable connection with a polyT termination sequence under the control of a human U6 RNA promoter. As used herein, "in operable connection with a polyT termination sequence" means that the nucleic acid sequences encoding the sense or antisense strands are immediately adjacent to the polyT termination signal in the 5' direction. During transcription of the miR sequences from the vector, the polyT termination signals act to terminate transcription.
[0081] In other embodiments of the treatment methods of the invention, an effective amount of at least one compound which inhibits miR expression can also be administered to the subject. As used herein, "inhibiting miR expression" means that the production of the active, mature form of miR gene product after treatment is less than the amount produced prior to treatment. One skilled in the art can readily determine whether miR expression has been inhibited in a cancer cell, using for example the techniques for determining miR transcript level discussed above for the diagnostic method Inhibition can occur at the level of gene expression (such as, by inhibiting transcription of a miR gene encoding the miR gene product) or at the level of processing (such as, by inhibiting processing of a miR precursor into a mature, active miR).
[0082] As used herein, an "effective amount" of a compound that inhibits miR expression is an amount sufficient to inhibit proliferation of a cancer cell in a subject suffering from a cancer associated with a cancer-associated chromosomal feature. One skilled in the art can readily determine an effective amount of an miR expression-inhibiting compound to be administered to a given subject, by taking into account factors, such as the size and weight of the subject; the extent of disease penetration; the age, health and sex of the subject; the route of administration; and whether the administration is regional or systemic.
[0083] For example, an effective amount of the expression-inhibiting compound can be based on the approximate weight of a tumor mass to be treated. The approximate weight of a tumor mass can be determined by calculating the approximate volume of the mass, wherein one cubic centimeter of volume is roughly equivalent to one gram. An effective amount based on the weight of a tumor mass can be between about 10-500 micrograms/gram of tumor mass, at least about 10 micrograms/gram of tumor mass, at least about 60 micrograms/gram of tumor mass, and at least about 100 micrograms/gram of tumor mass.
[0084] An effective amount of a compound that inhibits miR expression can also be based on the approximate or estimated body weight of a subject to be treated. Such effective amounts are administered parenterally or enterally, among others, as described herein. For example, an effective amount of the expression-inhibiting compound administered to a subject can range from about 5-3000 micrograms/kg of body weight, from about 700-1000 micrograms/kg of body weight, or it can be greater than about 1000 micrograms/kg of body weight.
[0085] One skilled in the art can also readily determine an appropriate dosage regimen for administering a compound that inhibits miR expression to a given subject. For example, an expression-inhibiting compound can be administered to the subject once (for example, as a single injection or deposition). Alternatively, an expression-inhibiting compound can be administered once or twice daily to a subject for a period of from about three to about twenty-eight days, more preferably from about seven to about ten days. In a particular dosage regimen, an expression-inhibiting compound is administered once a day for seven days. Where a dosage regimen comprises multiple administrations, it is understood that the effective amount of the expression-inhibiting compound administered to the subject can comprise the total amount of compound administered over the entire dosage regimen.
[0086] Suitable compounds for inhibiting miR gene expression include double-stranded RNA (such as short- or small-interfering RNA or "siRNA"), antisense nucleic acids, and enzymatic RNA molecules, such as ribozymes. Each of these compounds can be targeted to a given miR gene product and destroy or induce the destruction of the target miR gene product.
[0087] For example, expression of a given miR gene can be inhibited by inducing RNA interference of the miR gene with an isolated double-stranded RNA ("dsRNA") molecule which has at least 90%, for example at least 95%, at least 98%, at least 99% or 100%, sequence homology with at least a portion of the miR gene product. In a particular embodiment, the dsRNA molecule is a "short or small interfering RNA" or "siRNA."
[0088] siRNA useful in the present methods comprise short double-stranded RNA from about 17 nucleotides to about 29 nucleotides in length, preferably from about 19 to about 25 nucleotides in length. The siRNA comprise a sense RNA strand and a complementary antisense RNA strand annealed together by standard Watson-Crick base-pairing interactions (hereinafter "base-paired"). The sense strand comprises a nucleic acid sequence which is substantially identical to a nucleic acid sequence contained within the target miR gene product.
[0089] As used herein, a nucleic acid sequence in a siRNA which is "substantially identical" to a target sequence contained within the target mRNA is a nucleic acid sequence that is identical to the target sequence, or that differs from the target sequence by one or two nucleotides. The sense and antisense strands of the siRNA can comprise two complementary, single-stranded RNA molecules, or can comprise a single molecule in which two complementary portions are base-paired and are covalently linked by a single-stranded "hairpin" area.
[0090] The siRNA can also be altered RNA that differs from naturally-occurring RNA by the addition, deletion, substitution and/or alteration of one or more nucleotides. Such alterations can include addition of non-nucleotide material, such as to the end(s) of the siRNA or to one or more internal nucleotides of the siRNA, or modifications that make the siRNA resistant to nuclease digestion, or the substitution of one or more nucleotides in the siRNA with deoxyribonucleotides.
[0091] One or both strands of the siRNA can also comprise a 3' overhang. As used herein, a "3' overhang" refers to at least one unpaired nucleotide extending from the 3'-end of a duplexed RNA strand. Thus, in certain embodiments, the siRNA comprises at least one 3' overhang of from 1 to about 6 nucleotides (which includes ribonucleotides or deoxyribonucleotides) in length, from 1 to about 5 nucleotides in length, from 1 to about 4 nucleotides in length, or from about 2 to about 4 nucleotides in length. In a particular embodiment, the 3' overhang is present on both strands of the siRNA, and is 2 nucleotides in length. For example, each strand of the siRNA can comprise 3' overhangs of dithymidylic acid ("TT") or diuridylic acid ("uu").
[0092] The siRNA can be produced chemically or biologically, or can be expressed from a recombinant plasmid or viral vector, as described above for the isolated miR gene products. Exemplary methods for producing and testing dsRNA or siRNA molecules are described in U.S. Published Patent Application No. 2002/0173478 to Gewirtz and in U.S. Pat. No. 7,148,342 to Reich et al., the entire disclosures of which are incorporated herein by reference.
[0093] Expression of a given miR gene can also be inhibited by an antisense nucleic acid. As used herein, an "antisense nucleic acid" refers to a nucleic acid molecule that binds to target RNA by means of RNA-RNA or RNA-DNA or RNA-peptide nucleic acid interactions, which alters the activity of the target RNA. Antisense nucleic acids suitable for use in the present methods are single-stranded nucleic acids (for example, RNA, DNA, RNA-DNA chimeras, PNA) that generally comprise a nucleic acid sequence complementary to a contiguous nucleic acid sequence in an miR gene product. The antisense nucleic acid can comprise a nucleic acid sequence that is 50-100% complementary, 75-100% complementary, or 95-100% complementary to a contiguous nucleic acid sequence in an miR gene product. Nucleic acid sequences for the miR gene products are provided in Table 1. Without wishing to be bound by any theory, it is believed that the antisense nucleic acids activate RNase H or another cellular nuclease that digests the miR gene product/antisense nucleic acid duplex.
[0094] Antisense nucleic acids can also contain modifications to the nucleic acid backbone or to the sugar and base moieties (or their equivalent) to enhance target specificity, nuclease resistance, delivery or other properties related to efficacy of the molecule. Such modifications include cholesterol moieties, duplex intercalators, such as acridine, or one or more nuclease-resistant groups.
[0095] Antisense nucleic acids can be produced chemically or biologically, or can be expressed from a recombinant plasmid or viral vector, as described above for the isolated miR gene products. Exemplary methods for producing and testing are within the skill in the art; see, for example, Stein and Cheng (1993), Science 261:1004 and U.S. Pat. No. 5,849,902 to Woolf et al., the entire disclosures of which are incorporated herein by reference.
[0096] Expression of a given miR gene can also be inhibited by an enzymatic nucleic acid. As used herein, an "enzymatic nucleic acid" refers to a nucleic acid comprising a substrate binding region that has complementarity to a contiguous nucleic acid sequence of an miR gene product, and which is able to specifically cleave the miR gene product. The enzymatic nucleic acid substrate binding region can be, for example, 50-100% complementary, 75-100% complementary, or 95-100% complementary to a contiguous nucleic acid sequence in a miR gene product. The enzymatic nucleic acids can also comprise modifications at the base, sugar, and/or phosphate groups. An exemplary enzymatic nucleic acid for use in the present methods is a ribozyme.
[0097] The enzymatic nucleic acids can be produced chemically or biologically, or can be expressed from a recombinant plasmid or viral vector, as described above for the isolated miR gene products. Exemplary methods for producing and testing dsRNA or siRNA molecules are described in Werner and Uhlenbeck (1995), Nucl. Acids Res. 23:2092-96; Hammann et al. (1999), Antisense and Nucleic Acid Drug Dev. 9:25-31; and U.S. Pat. No. 4,987,071 to Cech et al, the entire disclosures of which are incorporated herein by reference.
[0098] Administration of at least one miR gene product, or at least one compound for inhibiting miR expression, will inhibit the proliferation of cancer cells in a subject who has a cancer associated with a cancer-associated chromosomal feature. As used herein, to "inhibit the proliferation of a cancer cell" means to kill the cell, or permanently or temporarily arrest or slow the growth of the cell. Inhibition of cancer cell proliferation can be inferred if the number of such cells in the subject remains constant or decreases after administration of the miR gene products or miR gene expression-inhibiting compounds. An inhibition of cancer cell proliferation can also be inferred if the absolute number of such cells increases, but the rate of tumor growth decreases.
[0099] The number of cancer cells in a subject's body can be determined by direct measurement, or by estimation from the size of primary or metastatic tumor masses. For example, the number of cancer cells in a subject can be measured by immunohistological methods, flow cytometry, or other techniques designed to detect characteristic surface markers of cancer cells.
[0100] The size of a tumor mass can be ascertained by direct visual observation, or by diagnostic imaging methods, such as X-ray, magnetic resonance imaging, ultrasound, and scintigraphy. Diagnostic imaging methods used to ascertain size of the tumor mass can be employed with or without contrast agents, as is known in the art. The size of a tumor mass can also be ascertained by physical means, such as palpation of the tissue mass or measurement of the tissue mass with a measuring instrument, such as a caliper.
[0101] The miR gene products or miR gene expression-inhibiting compounds can be administered to a subject by any means suitable for delivering these compounds to cancer cells of the subject. For example, the miR gene products or miR expression inhibiting compounds can be administered by methods suitable to transfect cells of the subject with these compounds, or with nucleic acids comprising sequences encoding these compounds. In one embodiment, the cells are transfected with a plasmid or viral vector comprising sequences encoding at least one miR gene product or miR gene expression inhibiting compound.
[0102] Transfection methods for eukaryotic cells are well known in the art, and include, for example, direct injection of the nucleic acid into the nucleus or pronucleus of a cell; electroporation; liposome transfer or transfer mediated by lipophilic materials; receptor-mediated nucleic acid delivery, bioballistic or particle acceleration; calcium phosphate precipitation, and transfection mediated by viral vectors.
[0103] For example, cells can be transfected with a liposomal transfer compound, such as, DOTAP (N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethyl-ammonium methylsulfate, Boehringer-Mannheim) or an equivalent, such as LIPOFECTIN. The amount of nucleic acid used is not critical to the practice of the invention; acceptable results may be achieved with 0.1-100 micrograms of nucleic acid/105 cells. For example, a ratio of about 0.5 micrograms of plasmid vector in 3 micrograms of DOTAP per 105 cells can be used.
[0104] A miR gene product or miR gene expression inhibiting compound can also be administered to a subject by any suitable enteral or parenteral administration route. Suitable enteral administration routes for the present methods include, for example, oral, rectal, or intranasal delivery. Suitable parenteral administration routes include, for example, intravascular administration (for example, intravenous bolus injection, intravenous infusion, intra-arterial bolus injection, intra-arterial infusion and catheter instillation into the vasculature); pen- and intra-tissue injection (for example, peri-tumoral and intra-tumoral injection, intra-retinal injection, or subretinal injection); subcutaneous injection or deposition, including subcutaneous infusion (such as by osmotic pumps); direct application to the tissue of interest, for example by a catheter or other placement device (for example, a retinal pellet or a suppository or an implant comprising a porous, non-porous, or gelatinous material); and inhalation. Particularly suitable administration routes are injection, infusion and direct injection into the tumor.
[0105] In the present methods, a miR gene product or miR gene product expression inhibiting compound can be administered to the subject either as naked RNA, in combination with a delivery reagent, or as a nucleic acid (for example, a recombinant plasmid or viral vector) comprising sequences that express the miR gene product or expression inhibiting compound. Suitable delivery reagents include, for example, the Mirus Transit TKO lipophilic reagent; lipofectin; lipofectamine; cellfectin; polycations (for example, polylysine), and liposomes.
[0106] Recombinant plasmids and viral vectors comprising sequences that express the miR gene products or miR gene expression inhibiting compounds, and techniques for delivering such plasmids and vectors to cancer cells, are discussed herein.
[0107] In a particular embodiment, liposomes are used to deliver a miR gene product or miR gene expression-inhibiting compound (or nucleic acids comprising sequences encoding them) to a subject. Liposomes can also increase the blood half-life of the gene products or nucleic acids. Suitable liposomes for use in the invention can be formed from standard vesicle-forming lipids, which generally include neutral or negatively charged phospholipids and a sterol, such as cholesterol. The selection of lipids is generally guided by consideration of factors, such as the desired liposome size and half-life of the liposomes in the blood stream. A variety of methods are known for preparing liposomes, for example, as described in Szoka et al. (1980), Ann. Rev. Biophys. Bioeng. 9:467; and U.S. Pat. Nos. 4,235,871, 4,501,728, 4,837,028, and 5,019,369, the entire disclosures of which are incorporated herein by reference.
[0108] The liposomes for use in the present methods can comprise a ligand molecule that targets the liposome to cancer cells. Ligands which bind to receptors prevalent in cancer cells, such as monoclonal antibodies that bind to tumor cell antigens, are preferred.
[0109] The liposomes for use in the present methods can also be modified so as to avoid clearance by the mononuclear macrophage system ("MMS") and reticuloendothelial system ("RES"). Such modified liposomes have opsonization-inhibition moieties on the surface or incorporated into the liposome structure. In a particularly preferred embodiment, a liposome of the invention can comprise both opsonization-inhibition moieties and a ligand.
[0110] Opsonization-inhibiting moieties for use in preparing the liposomes of the invention are typically large hydrophilic polymers that are bound to the liposome membrane. As used herein, an opsonization inhibiting moiety is "bound" to a liposome membrane when it is chemically or physically attached to the membrane, for example, by the intercalation of a lipid-soluble anchor into the membrane itself, or by binding directly to active groups of membrane lipids. These opsonization-inhibiting hydrophilic polymers form a protective surface layer that significantly decreases the uptake of the liposomes by the MMS and RES; for example, as described in U.S. Pat. No. 4,920,016, the entire disclosure of which is incorporated herein by reference.
[0111] Opsonization inhibiting moieties suitable for modifying liposomes are preferably water-soluble polymers with a number-average molecular weight from about 500 to about 40,000 daltons, and more preferably from about 2,000 to about 20,000 daltons. Such polymers include polyethylene glycol (PEG) or polypropylene glycol (PPG) derivatives; for example, methoxy PEG or PPG, and PEG or PPG stearate; synthetic polymers, such as polyacrylamide or poly N-vinyl pyrrolidone; linear, branched, or dendrimeric polyamidoamines; polyacrylic acids; polyalcohols, for example, polyvinylalcohol and polyxylitol to which carboxylic or amino groups are chemically linked, as well as gangliosides, such as ganglioside GM1. Copolymers of PEG, methoxy PEG, or methoxy PPG, or derivatives thereof, are also suitable. In addition, the opsonization inhibiting polymer can be a block copolymer of PEG and either a polyamino acid, polysaccharide, polyamidoamine, polyethyleneamine, or polynucleotide. The opsonization inhibiting polymers can also be natural polysaccharides containing amino acids or carboxylic acids, for example, galacturonic acid, glucuronic acid, mannuronic acid, hyaluronic acid, pectic acid, neuraminic acid, alginic acid, carrageenan; aminated polysaccharides or oligosaccharides (linear or branched); or carboxylated polysaccharides or oligosaccharides, for example, reacted with derivatives of carbonic acids with resultant linking of carboxylic groups. Preferably, the opsonization-inhibiting moiety is a PEG, PPG, or derivatives thereof. Liposomes modified with PEG or PEG-derivatives are sometimes called "PEGylated liposomes."
[0112] The opsonization inhibiting moiety can be bound to the liposome membrane by any one of numerous well-known techniques. For example, an N-hydroxysuccinimide ester of PEG can be bound to a phosphatidyl-ethanolamine lipid-soluble anchor, and then bound to a membrane. Similarly, a dextran polymer can be derivatized with a stearylamine lipid-soluble anchor via reductive amination using Na(CN)BH3 and a solvent mixture, such as tetrahydrofuran and water in a 30:12 ratio at 60° C.
[0113] Liposomes modified with opsonization-inhibition moieties remain in the circulation much longer than unmodified liposomes. For this reason, such liposomes are sometimes called "stealth" liposomes. Stealth liposomes are known to accumulate in tissues fed by porous or "leaky" microvasculature. Thus, tissue characterized by such microvasculature defects, for example solid tumors, will efficiently accumulate these liposomes; see Gabizon, et al. (1988), Proc. Natl. Acad. Sci., U.S.A., 18:6949-53. In addition, the reduced uptake by the RES lowers the toxicity of stealth liposomes by preventing significant accumulation of the liposomes in the liver and spleen. Thus, liposomes that are modified with opsonization-inhibition moieties are particularly suited to deliver the miR gene products or miR gene expression inhibition compounds (or nucleic acids comprising sequences encoding them) to tumor cells.
[0114] The miR gene products or miR gene expression inhibition compounds can be formulated as pharmaceutical compositions, sometimes called "medicaments," prior to administering them to a subject, according to techniques known in the art. Accordingly, the invention encompasses pharmaceutical compositions for treating breast cancer. In one embodiment, the pharmaceutical compositions comprise at least one isolated miR gene product and a pharmaceutically-acceptable carrier. In a particular embodiment, the at least one miR gene product corresponds to a miR gene product that has a decreased level of expression in breast cancer cells relative to suitable control cells. In certain embodiments the isolated miR gene product is selected from the group consisting of miR-145, miR-10b, miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, miR-101-1, miR-143 and combinations thereof.
[0115] In other embodiments, the pharmaceutical compositions of the invention comprise at least one miR expression inhibition compound. In a particular embodiment, the at least one miR gene expression inhibition compound is specific for a miR gene whose expression is greater in breast cancer cells than control cells. In certain embodiments, the miR gene expression inhibition compound is specific for one or more miR gene products selected from the group consisting of miR-21, miR-155, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-213, let-7i (let-7d-v2), miR-122a, miR-128b, miR-136, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210, miR-213 and combinations thereof.
[0116] Pharmaceutical compositions of the present invention are characterized as being at least sterile and pyrogen-free. As used herein, "pharmaceutical formulations" include formulations for human and veterinary use. Methods for preparing pharmaceutical compositions of the invention are within the skill in the art, for example as described in Remington's Pharmaceutical Science, 17th ed., Mack Publishing Company, Easton, Pa. (1985), the entire disclosure of which is incorporated herein by reference.
[0117] The present pharmaceutical formulations comprise at least one miR gene product or miR gene expression inhibition compound (or at least one nucleic acid comprising sequences encoding them) (for example, 0.1 to 90% by weight), or a physiologically acceptable salt thereof, mixed with a pharmaceutically-acceptable carrier. The pharmaceutical formulations of the invention can also comprise at least one miR gene product or miR gene expression inhibition compound (or at least one nucleic acid comprising sequences encoding them) which are encapsulated by liposomes and a pharmaceutically-acceptable carrier. In one embodiment, the pharmaceutical compositions comprise a miR gene or gene product that is not miR-15, miR-16, miR-143 and/or miR-145.
[0118] Especially suitable pharmaceutically-acceptable carriers are water, buffered water, normal saline, 0.4% saline, 0.3% glycine, hyaluronic acid and the like.
[0119] In a particular embodiment, the pharmaceutical compositions of the invention comprise at least one miR gene product or miR gene expression inhibition compound (or at least one nucleic acid comprising sequences encoding them) which is resistant to degradation by nucleases. One skilled in the art can readily synthesize nucleic acids which are nuclease resistant, for example by incorporating one or more ribonucleotides that are modified at the 2'-position into the miR gene products. Suitable 2'-modified ribonucleotides include those modified at the 2'-position with fluoro, amino, alkyl, alkoxy, and O-allyl.
[0120] Pharmaceutical compositions of the invention can also comprise conventional pharmaceutical excipients and/or additives. Suitable pharmaceutical excipients include stabilizers, antioxidants, osmolality adjusting agents, buffers, and pH adjusting agents. Suitable additives include, for example, physiologically biocompatible buffers (for example, tromethamine hydrochloride), additions of chelants (such as, for example, DTPA or DTPA-bisamide) or calcium chelate complexes (such as, for example, calcium DTPA, CaNaDTPA-bisamide), or, optionally, additions of calcium or sodium salts (for example, calcium chloride, calcium ascorbate, calcium gluconate or calcium lactate). Pharmaceutical compositions of the invention can be packaged for use in liquid form, or can be lyophilized.
[0121] For solid pharmaceutical compositions of the invention, conventional nontoxic solid pharmaceutically-acceptable carriers can be used; for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, talcum, cellulose, glucose, sucrose, magnesium carbonate, and the like.
[0122] For example, a solid pharmaceutical composition for oral administration can comprise any of the carriers and excipients listed above and 10-95%, preferably 25%-75%, of the at least one miR gene product or miR gene expression inhibition compound (or at least one nucleic acid comprising sequences encoding them). A pharmaceutical composition for aerosol (inhalational) administration can comprise 0.01-20% by weight, preferably 1%-10% by weight, of the at least one miR gene product or miR gene expression inhibition compound (or at least one nucleic acid comprising sequences encoding them) encapsulated in a liposome as described above, and a propellant. A carrier can also be included as desired; for example, lecithin for intranasal delivery.
[0123] The invention also encompasses methods of identifying an anti-breast cancer agent, comprising providing a test agent to a cell and measuring the level of at least one miR gene product in the cell. In one embodiment, the method comprises providing a test agent to a cell and measuring the level of at least one miR gene product associated with decreased expression levels in breast cancer cells. An increase in the level of the miR gene product in the cell, relative to a suitable control cell, is indicative of the test agent being an anti-breast cancer agent. In a particular embodiment, at least one miR gene product associated with decreased expression levels in breast cancer cells is selected from the group consisting of miR-145, miR-10b, miR-123 (miR-126), miR-140-as, miR-125a, miR-125b-1, miR-125b-2, miR-194, miR-204, let-7a-2, let-7a-3, let-7d (let-7d-v1), let-7f-2, miR-101-1, miR-143 and combinations thereof.
[0124] In other embodiments the method comprises providing a test agent to a cell and measuring the level of at least one miR gene product associated with increased expression levels in breast cancer cells. A decrease in the level of the miR gene product in the cell, relative to a suitable control cell, is indicative of the test agent being an anti-breast cancer agent. In a particular embodiment, at least one miR gene product associated with increased expression levels in breast cancer cells is selected from the group consisting of miR-21, miR-155, miR-009-1 (miR131-1), miR-34 (miR-170), miR-102 (miR-29b), miR-213, let-7i (let-7d-v2), miR-122a, miR-128b, miR-136, miR-149, miR-191, miR-196-1, miR-196-2, miR-202, miR-203, miR-206, miR-210, miR-213 and combinations thereof.
[0125] Suitable agents include, but are not limited to drugs (for example, small molecules, peptides), and biological macromolecules (for example, proteins, nucleic acids). The agent can be produced recombinantly, synthetically, or it may be isolated (i.e., purified) from a natural source. Various methods for providing such agents to a cell (for example, transfection) are well known in the art, and several of such methods are described hereinabove. Methods for detecting the expression of at least one miR gene product (for example, Northern blotting, in situ hybridization, RT-PCR, expression profiling) are also well known in the art. Several of these methods are also described hereinabove.
[0126] The invention will now be illustrated by the following non-limiting examples.
Example 1
Identification of a microRNA Expression Signature that Discriminates Breast Cancer Tissues from Normal Tissues
[0127] Materials and Methods
[0128] Breast Cancer Samples and Cell Lines.
[0129] RNAs from primary tumors were obtained from 76 samples collected at the University of Ferrara (Italy), Istituto Nazionale dei Tumori, Milano (Italy) and Thomas Jefferson University (Philadelphia, Pa.). Clinico-pathological information was available for 58 tumor samples. RNA from normal samples consisted of 6 pools of RNA from 5 normal breast tissues each, as well as RNA from 4 additional single breast tissues. Breast cancer RNAs were also obtained from the following cell lines: Hs578-T, MCF7, T47D, BT20, SK-BR-3, HBL100, HCC2218, MDA-MB-175, MDA-MB-231, MDA-MB-361, MDA-MB-435, MDA-MB-436, MDA-MB-453 and MDAMB-468.
[0130] miRNA Microarray.
[0131] Total RNA isolation was performed with Trizol Reagent (Invitrogen) according to the manufacturer's instructions. RNA labeling and hybridization on microRNA microarray chips was performed as previously described (Liu, C.-G., et al., Proc. Natl. Acad. Sci. U.S.A. 101:9740-9744 (2004)). Briefly, 5 μg of RNA from each sample was labeled with biotin during reverse transcription using random hexamers. Hybridization was carried out on a miRNA microarray chip (KCl version 1.0), which contains 368 probes, including 245 human and mouse miRNA genes, in triplicate. Hybridization signals were detected by binding of biotin to a Streptavidin-Alexa647 conjugate using a Perkin-Elmer ScanArray XL5K. Scanner images were quantified by the Quantarray software (Perkin Elmer).
[0132] Statistical and bioinformatic analysis of microarray data. Raw data were normalized and analyzed using the GeneSpring® software, version 7.2 (SiliconGenetics, Redwood City, Calif.). Expression data were median centered. Statistical comparisons were performed by ANOVA (Analysis of Variance), using the Benjamini and Hochberg correction for reduction of false positives. Prognostic miRNAs for tumor or normal class prediction were determined using both the PAM software (Prediction Analysis of Microarrays) (Tibshirani, R., et al. Proc. Natl. Acad. Sci. U.S.A. 99:6567-6572 (2002)) and the Support Vector Machine (Furey, T. S., et al. Bioinformatics 16: 906-914 (2000)) software. Both algorithms were used for Cross-validation and Test-set prediction. All data were submitted using MIAMExpress to the Array Express database.
[0133] Northern Blotting.
[0134] Northern blot analysis was performed as previously described (Calin, G. A., et al., Proc. Natl. Acad. Sci. U.S.A. 99:15524-29 (2002)). RNA samples (10 μg each) were electrophoresed on 15% acrylamide, 7 M urea Criterion pre-casted gels (Bio-Rad) and transferred onto Hybond-N+ membrane (Amersham Pharmacia Biotech). The hybridization was performed at 37° C. in 7% sodium dodecyl sulfate (SDS)/0.2M Na2PO4 (pH 7.0) for 16 hours. Membranes were washed twice at 42° C. with 2× standard saline phosphate (0.18 M NaCl/10 mM phosphate, pH 7.4), supplemented with 1 mM EDTA (SSPE) and 0.1% SDS, and twice with 0.5×SSPE/0.1% SDS. Oligonucleotide probes were complementary to the sequence of the corresponding mature microRNA (see Sanger miR Registry): miR-21 5'-TCA ACA TCA GTC TGA TAA GCT A-3' (SEQ ID NO:287); miR-125b1: 5'-TCA CAA GTT AGG GTC TCA GGG A-3' (SEQ ID NO:288); miR-145: 5'-AAG GGA TTC CTG GGA AAA CTG GAC-3' (SEQ ID NO:289). An oligonucleotide that was complementary to the U6 RNA (5'-GCA GGG GCC ATG CTA ATC TTC TCT GTA TCG-3' (SEQ ID NO:290)) was used for normalizing expression levels. 200 ng of each probe was end labeled with 100 mCi [gamma-32P]-ATP using a polynucleotide kinase (Roche). Northern Blots were stripped in a boiling 0.1% SDS solution for 10 minutes before re-hybridization.
[0135] Results
[0136] A microRNA microarray (Liu, C.-G., et al., Proc. Natl. Acad. Sci. U.S.A. 101:9740-9744 (2004)) was used to generate microRNA expression profiles for 10 normal and 76 neoplastic breast tissues. Each tumor sample was derived from a single specimen, while 6 of the 10 normal samples consisted of pools of RNA made from five different normal breast tissues. Hence, 34 normal breast samples were actually examined in the study.
[0137] To identify miRNAs that were differentially-expressed between normal and tumor samples, and, therefore, can be used to distinguish normal from cancerous breast tissues, analyses of variance and class prediction statistical tools were utilized. Results of the ANOVA analysis on normalized data generated a profile of differentially-expressed miRNAs (p<0.05) between normal and cancerous breast tissues (Table 2). Cluster analysis, based on differentially-expressed miRNA, generated a tree having a clear distinction between normal and cancer tissues (FIG. 1A).
[0138] To accurately identify a set of predictive miRNAs capable of differentiating normal from breast cancer tissues, we used Support Vector Machine (GeneSpring software) and PAM (Prediction Analysis of Microarrays). Results from the two class prediction analyses largely overlapped (Table 3 and FIG. 1B). Among the miRNAs listed in Table 3, 11 of 15 have an ANOVA p-value of less than 0.05. To confirm the results obtained by microarray analysis, we performed Northern blot analysis to assess expression levels for a subset of microRNAs, namely, mir-125b, mir-145 and mir-21, that were differentially-expressed in normal and cancerous breast tissues. Northern blot analysis confirmed results obtained by microarray analysis. In many cases, expression differences appeared stronger than those anticipated by the microarray studies (FIG. 1C).
TABLE-US-00002 TABLE 2 miRNAs differentially-expressed between breast carcinoma and normal breast tissue. Breast Cancer Normal Breast Median Range Median Range P-value Normalized Min Max Normalized Min Max let-7a-2 1.94E-02 1.67 0.96 - 6.21 2.30 1.34 - 5.00 let-7a-3 4.19E-02 1.28 0.81 - 3.79 1.58 1.02 - 2.91 let-7d (= 7d-v1) 4.81E-03 0.90 0.59 - 1.54 1.01 0.83 - 1.25 let-7f-2 6.57E-03 0.84 0.61 - 1.58 0.92 0.76 - 1.03 let-7f (= let-7d-v2) 3.38E-02 2.05 1.02 - 7.49 1.53 1.01 - 3.47 mir-009-1 (mir-131-1) 9.12E-03 1.38 0.69 - 4.18 1.01 0.81 - 2.44 mir-010b 4.49E-02 1.11 0.69 - 4.78 1.70 0.96 - 8.32 mir-021 4.67E-03 1.67 0.66 - 28.43 1.08 0.80 - 2.31 mir-034 (=mir-17D) 1.06E-02 1.87 0.70 - 8.40 1.09 0.65 - 3.17 mir-101-1 4.15E-03 0.83 0.52 - 1.28 0.90 0.77 - 1.05 mir-122a 3.43E-03 2.21 0.93 - 8.08 1.48 1.06 - 3.67 mir-125a 3.28E-03 1.20 0.69 - 2.35 1.73 1.21 - 3.34 mir-125b-1 2.85E-02 1.30 0.55 - 8.85 2.87 1.45 - 18.38 mir-125b-2 2.33E-02 1.26 0.69 - 8.29 2.83 1.40 - 18.78 mir-126b 1.60E-02 1.12 0.68 - 7.34 1.02 0.89 - 1.27 mir-136 2.42E-03 1.32 0.74 - 10.28 1.06 0.76 - 1.47 mir-143 7.11E-03 0.87 0.68 - 1.33 0.98 0.81 - 1.17 mir-145 4.02E-03 1.52 0.92 - 8.46 3.61 1.65 - 14.45 mir-149 2.75E-02 1.11 0.53 - 1.73 1.03 0.83 - 1.22 mir-155(BIC) 1.24E-03 1.75 0.95 - 11.45 1.37 1.11 - 1.88 mir-191 4.28E-02 5.17 1.03 - 37.81 3.12 1.45 - 14.58 mir-196-1 1.07E-02 1.20 0.57 - 3.95 0.95 0.66 - 1.75 mir-196-2 1.16E-03 1.46 0.57 - 5.55 1.04 0.79 - 1.80 mir-202 1.25E-02 1.05 0.71 - 2.03 0.89 0.65 - 1.20 mir-203 4.08E-07 1.12 0.50 - 5.89 0.86 0.71 - 1.04 mir-204 2.15E-03 0.78 0.48 - 1.04 0.89 0.72 - 1.08 mir-206 1.42E-02 2.55 1.22 - 8.42 1.95 1.34 - 3.22 mir-210 6.40E-13 1.60 0.98 - 12.13 1.12 0.97 - 1.29 mir-213 1.08E-02 3.72 1.42 - 40.83 2.47 1.35 - 5.91
TABLE-US-00003 TABLE 3 Normal and tumor breast tissues class predictor microRNAs Median expression ANOVAa SVM prediction PAM scorec miRNA name Cancer Normal Probability strengthb Cancer Normal Chromos map mir-009-1 1.36 1.01 0.0091 8.05 0.011 -0.102 1q22 mir-010b 1.11 1.70 0.0449 8.70 -0.032 0.299 2q31 mir-021 1.67 1.08 0.0047 10.20 0.025 -0.235 17q23.2 mir-034 1.67 1.09 0.0108 8.05 0.011 -0.106 1p36.22 mir-102 (mir-29b) 1.36 1.14 >0.10 8.92 0.000 -0.004 1q32.2-32.3 mir-123 (mir-126) 0.92 1.13 0.0940 9.13 -0.015 0.138 9q34 mir-125a 1.20 1.73 0.0033 8.99 -0.040 0.381 19q13.4 mir-125b-1 1.30 2.87 0.0265 14.78 -0.096 0.915 11q24.1 mir-125b-2 1.26 2.63 0.0233 17.62 -0.106 1.006 21q11.2 mir-140-as 0.93 1.10 0.0695 11.01 -0.005 0.050 16q22.1 mir-145 1.52 3.61 0.0040 12.93 -0.158 1.502 5q32-33 mir-155(BIC) 1.75 1.37 0.0012 10.92 0.003 -0.030 21q21 mir-194 0.96 1.09 >0.10 11.12 -0.025 0.234 1q41 mir-204 0.78 0.89 0.0022 8.10 -0.015 0.144 9q21.1 mir-213 3.72 2.47 0.0108 9.44 0.023 -0.220 1q31.3-q32.1 aAnalysis of Variance (Welch t-test in Genespring software package) as calculated in Table 2. bSupport Vector Machine prediction analysis tool (from Genespring 7.2 software package).
Prediction strengths are calculated as negative natural log of the probability to predict the observed number of samples, in one of the two classes, by chance. The higher is the score, the best is the prediction strength. c--Centroid scores for the two classes of the Prediction Analysis of Microarrays (Tibshirani, R., et al. Proc. Natl. Acad. Sci. U.S.A. 99:6567-6572 (2002)).
[0139] Of the 29 miRNAs whose expression is significantly (p<0.05) deregulated according to the microarray analysis, a set of 15 miRNAs were able to correctly predict the nature of the sample analyzed (i.e., normal vs. tumor) with 100% accuracy. Among the differentially-expressed miRNAs, miR-10b, miR-125b, miR145, miR-21 and miR-155 were the most consistently deregulated miRNAs in breast cancer samples. Three of these, namely, miR-10b, miR-125b and miR-145, were down-regulated, while the remaining two, miR-21 and miR-155, were up-regulated, suggesting that they might act as tumor suppressor genes or oncogenes, respectively.
Example 2
Determination of Putative Gene Targets of miRNAs that are Deregulated in Breast Cancer Tissues
[0140] At present, the lack of knowledge about bona fide miRNA gene targets hampers a full understanding of which biological functions are deregulated in cancers characterized by aberrant miRNA expression. To identify putative targets of the most significantly de-regulated miRNAs from our study: miR-10b, miR125b, miR-145, miR-21 and miR-155 (see Example 1), we utilized multiple computational approaches. In particular, the analysis was performed using three algorithms, miRanda, TargetScan and PicTar, which are used to predict human miRNA gene targets (Enright, A. J., et al. Genome Biol. 5:R1 (2003); Lewis, B. P. et al., Cell 115:787-798 (2003); Krek, A., et al., Nat. Genet. 37:495-500 (2005)). The results obtained using each of the three algorithms were cross-referenced with one another to validate putative targets, and only targets that were identified by at least 2 of the 3 algorithms were considered. Results of this analysis are presented in Table 4.
[0141] Several genes with potential oncogenic functions were identified as putative targets of miRNAs that are down-regulated in breast cancer samples. Notably, oncogenes were identified as targets of miR-10b (for example, FLT1, the v-crk homolog, the growth factor BDNF and the transducing factor SHC1), miR-125b (for example, YES, ETS1, TEL, AKT3, the growth factor receptor FGFR2 and members of the mitogen-activated signal transduction pathway VTS58635, MAP3K10, MAP3K11, MAPK14), and miR-145 (for example, MYCN, FOS, YES and FLI1, integration site of Friend leukemia virus, cell cycle promoters, such as cyclins D2 and L1, MAPK transduction proteins, such as MAP3K3 and MAP4K4). The proto-oncogene, YES, and the core-binding transcription factor, CBFB, were determined to be potential targets of both miR-125 and miR-145.
[0142] Consistent with these findings, multiple tumor suppressor genes were identified as targets of miR-21 and miR-155, miRNAs that are up-regulated in breast cancer cells. For miR-21, the TGFB gene was predicted as target by all three methods. For miR-155, potential targets included the tumor suppressor genes, SOCS1 and APC, and the kinase, WEE1, which blocks the activity of Cdc2 and prevents entry into mitosis. The hypoxia inducible factor, HIF1A, was also a predicted target of miR-155. Notably, the tripartite motif-containing protein TRIM2, the proto-oncogene, SKI, and the RAS homologs, RAB6A and RAB6C, were found as potential targets of both miR-21 and miR-155.
TABLE-US-00004 TABLE 4 Putative gene targets of differentially-expressed miRNA identified by at least two prediction methods Gene Prediction miRNA Genbank Symbol Gene Name algorithm Gene Ontology condensed miR- AL117516 38596 strand-exchange protein 1 P + T exonuclease activity|nucleus 10b miR- NM_004915 ABCG1 ATP-binding cassette, P + T ATP binding|ATPase activity|ATPase activity, 10b sub-family G (WHITE), coupled to transmembrane movement of member 1 substances|L-tryptophan transporter activity|cholesterol homeostasis|cholesterol metabolism|detection of hormone stimulus|integral to plasma membrane|lipid transport|membrane|membrane fraction|permease activity|protein dimerization activity|purine nucleotide transporter activity|response to organic substance miR- NM_001148 ANK2 ankyrin 2, neuronal P + T actin 10b cytoskeleton|membrane|metabolism|oxidoreductase activity|protein binding|signal transduction|structural constituent of cytoskeleton miR- NM_020987 ANK3 ankyrin 3, node of P + T Golgi apparatus|cytoskeletal 10b Ranvier (ankyrin G) anchoring|cytoskeleton|cytoskeleton|endoplasmic reticulum|protein binding|protein targeting|signal transduction|structural constituent of cytoskeleton miR- NM_016376 ANKHZN ANKHZN protein P + T endocytosis|endosome membrane|membrane|protein 10b binding|zinc ion binding miR- NM_006380 APPBP2 amyloid beta precursor P + T binding|cytoplasm|intracellular protein 10b protein (cytoplasmic transport|membrane|microtubule associated tail) binding protein 2 complex|microtubule motor activity|nucleus miR- NM_006321 ARIH2 ariadne homolog 2 P + T development|nucleic acid binding|nucleus|protein 10b (Drosophila) ubiquitination|ubiquitin ligase complex|ubiquitin- protein ligase activity|zinc ion binding miR- NM_001668 ARNT aryl hydrocarbon P + T aryl hydrocarbon receptor nuclear translocator 10b receptor nuclear activity|nucleus|nucleus|protein-nucleus import, translocator translocation|receptor activity|regulation of transcription, DNA-dependent|signal transducer activity|signal transduction|transcription coactivator activity|transcription factor activity|transcription factor activity miR- AI829840 ASXL1 ESTs, Weakly similar P + T nucleus|regulation of transcription, DNA- 10b to SFRB_HUMAN dependent|transcription Splicing factor arginine/serine-rich 11 (Arginine-rich 54 kDa nuclear protein) (P54) [H. sapiens] miR- NM_021813 BACH2 BTB and CNC P + T DNA binding|nucleus|protein binding|regulation of 10b homology 1, basic transcription, DNA-dependent|transcription leucine zipper transcription factor 2 miR- NM_013450 BAZ2B bromodomain adjacent P + T DNA binding|nucleus|regulation of transcription, 10b to zinc finger domain, DNA-dependent|transcription 2B miR- NM_001706 BCL6 B-cell CLL/lymphoma P + T inflammatory response|mediator complex|negative 10b 6 (zinc finger protein regulation of transcription from RNA polymerase II 51) promoter|nucleus|positive regulation of cell proliferation|protein binding|regulation of transcription, DNA- dependent|transcription|transcription factor activity|zinc ion binding miR- NM_001709 BDNF brain-derived P + T growth factor activity|growth factor 10b neurotrophic factor activity|neurogenesis miR- NM_006624 BS69 adenovirus 5 E1A P + T DNA binding|cell cycle|cell proliferation|negative 10b binding protein regulation of cell cycle|negative regulation of transcription from RNA polymerase II promoter|nucleus|regulation of transcription, DNA- dependent|transcription miR- AF101784 BTRC beta-transducin repeat P + T Wnt receptor signaling pathway|endoplasmic 10b containing reticulum|ligase activity|signal transduction|ubiquitin conjugating enzyme activity|ubiquitin cycle|ubiquitin- dependent protein catabolism miR- NM_005808 C3orf8 HYA22 protein P + T biological_process unknown|molecular_function 10b unknown|nucleus miR- BF111268 CAMK2G calcium/calmodulin- P + T ATP binding|ATP binding|calcium- and calmodulin- 10b dependent protein dependent protein kinase activity|calcium-dependent kinase (CaM kinase) II protein serine/threonine phosphatase gamma activity|calmodulin binding|cellular_component unknown|insulin secretion|kinase activity|protein amino acid phosphorylation|protein amino acid phosphorylation|protein serine/threonine kinase activity|protein-tyrosine kinase activity|signal transduction|transferase activity miR- NM_020184 CNNM4 cyclin M4 P + T 10b miR- NM_022730 COPS7B COP9 constitutive P + T signalosome complex 10b photomorphogenic homolog subunit 7B (Arabidopsis) miR- NM_016823 CRK v-crk sarcoma virus P + T SH3/SH2 adaptor activity|actin cytoskeleton 10b CT10 oncogene organization and biogenesis|cell homolog (avian) motility|cytoplasm|intracellular signaling cascade|nucleus|regulation of transcription from RNA polymerase II promoter miR- NM_020248 CTNNBIP1 catenin, beta interacting P + T Wnt receptor signaling pathway|beta-catenin 10b protein 1 binding|cell proliferation|development|nucleus|regulation of transcription, DNA-dependent|signal transduction miR- NM_018959 DAZAP1 DAZ associated protein 1 P + T RNA binding|cell differentiation|nucleotide 10b binding|nucleus|spermatogenesis miR- AL136828 DKFZP434K0427 hypothetical protein P + T cation transport|cation transporter activity 10b DKFZp434K0427 miR- R20763 DKFZp547J036 ELAV (embryonic P + T 10b lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) miR- AF009204 DLGAP2 discs, large P + T cell-cell signaling|membrane|nerve-nerve synaptic 10b (Drosophila) homolog- transmission|neurofilament|protein binding associated protein 2 miR- NM_001949 E2F3 E2F transcription factor 3 P + T nucleus|protein binding|regulation of cell 10b cycle|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcription factor complex|transcription initiation from RNA polymerase II promoter miR- NM_022659 EBF2 early B-cell factor 2 P + T DNA binding|development|nucleus|regulation of 10b transcription, DNA-dependent|transcription miR- NM_004432 ELAVL2 ELAV (embryonic P + T RNA binding|mRNA 3'-UTR binding|nucleotide 10b lethal, abnormal vision, binding|regulation of transcription, DNA-dependent Drosophila)-like 2 (Hu antigen B) miR- NM_001420 ELAVL3 ELAV (embryonic P + T RNA binding|cell differentiation|mRNA 3'-UTR 10b lethal, abnormal vision, binding|neurogenesis|nucleotide binding Drosophila)-like 3 (Hu antigen C) miR- NM_004438 EPHA4 EphA4 P + T ATP binding|ephrin receptor activity|integral to 10b plasma membrane|membrane|protein amino acid phosphorylation|receptor activity|signal transduction|transferase activity|transmembrane receptor protein tyrosine kinase signaling pathway miR- AL035703 EPHA8; EphA8 P + T 10b EEK; HEK3; Hek3; KIAA1459 miR- NM_004468 FHL3 four and a half LIM P + T muscle development|zinc ion binding 10b domains 3 miR- NM_024679 FLJ11939 hypothetical protein P + T 10b FLJ11939 miR- AI742838 FLJ32122 hypothetical protein P + T GTP binding|GTPase binding|guanyl-nucleotide 10b FLJ32122 exchange factor activity miR- AL040935 FLJ33957 hypothetical protein P + T protein binding 10b FLJ33957 miR- AA058828 FLT1 ESTs P + T ATP binding|angiogenesis|cell 10b differentiation|extracellular space|integral to plasma membrane|membrane|positive regulation of cell proliferation|pregnancy|protein amino acid phosphorylation|receptor activity|transferase activity|transmembrane receptor protein tyrosine kinase signaling pathway|vascular endothelial growth factor receptor activity miR- NM_004860 FXR2 fragile X mental P + T RNA binding|cytoplasm|cytosolic large ribosomal 10b retardation, autosomal subunit (sensu Eukaryota)|nucleus homolog 2 miR- NM_020474 GALNT1 UDP-N-acetyl-alpha-D- P + T Golgi apparatus|O-linked glycosylation|integral to 10b galactosamine:polypeptide membrane|manganese ion binding|polypeptide N- N- acetylgalactosaminyltransferase activity|sugar acetylgalactosaminyl- binding|transferase activity, transferring glycosyl transferase 1 groups (GalNAc-T1) miR- D87811 GATA6 GATA binding protein 6 P + T muscle development|nucleus|positive regulation of 10b transcription|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcriptional activator activity|zinc ion binding miR- NM_000840 GRM3 glutamate receptor, P + T G-protein coupled receptor protein signaling 10b metabotropic 3 pathway|integral to plasma membrane|membrane|metabotropic glutamate, GABA-B-like receptor activity|negative regulation of adenylate cyclase activity|receptor activity|signal transduction|synaptic transmission miR- NM_005316 GTF2H1 general transcription P + T DNA repair|[RNA-polymerase]-subunit kinase 10b factor IIH, polypeptide activity|general RNA polymerase II transcription 1, 62 kDa factor activity|nucleus|regulation of cyclin dependent protein kinase activity|regulation of transcription, DNA-dependent|transcription|transcription factor TFIIH complex|transcription from RNA polymerase II promoter miR- AF232772 HAS3 hyaluronan synthase 3 P + T carbohydrate metabolism|hyaluronan synthase 10b activity|integral to plasma membrane|transferase activity, transferring glycosyl groups miR- AL023584 HIVEP2 human P + T 10b immunodeficiency virus type I enhancer binding protein 2 miR- S79910 HOXA1 homeo box A1 P + T RNA polymerase II transcription factor 10b activity|development|nucleus|regulation of transcription, DNA-dependent|transcription factor activity miR- NM_030661 HOXA3 homeo box A3 P + T development|nucleus|regulation of transcription, 10b DNA-dependent|transcription factor activity miR- AW299531 HOXD10 homeo box D10 P + T RNA polymerase II transcription factor 10b activity|development|nucleus|regulation of transcription, DNA-dependent|transcription factor activity miR- BF031714 HYA22 HYA22 protein P + T 10b miR- NM_001546 ID4 inhibitor of DNA P + T nucleus|regulation of transcription from RNA 10b binding 4, dominant polymerase II promoter|transcription corepressor negative helix-loop- activity helix protein miR- NM_014333 IGSF4 immunoglobulin P + T 10b superfamily, member 4 miR- NM_014271 IL1RAPL1 interleukin 1 receptor P + T integral to membrane|learning and/or
10b accessory protein-like 1 memory|membrane|signal transduction|transmembrane receptor activity miR- D87450 KIAA0261 KIAA0261 protein P + T 10b miR- AL117518 KIAA0978 KIAA0978 protein P + T nucleus|regulation of transcription, DNA- 10b dependent|transcription miR- AK025960 KIAA1255 KIAA1255 protein P + T endocytosis|endosome membrane|membrane|protein 10b binding|zinc ion binding miR- AB037797 KIAA1376 KIAA1376 protein P + T 10b miR- NM_004795 KL klotho P + T beta-glucosidase activity|carbohydrate 10b metabolism|extracellular space|glucosidase activity|integral to membrane|integral to plasma membrane|membrane fraction|signal transducer activity|soluble fraction miR- NM_015995 KLF13 Kruppel-like factor 13 P + T DNA binding|RNA polymerase II transcription factor 10b activity|nucleus|regulation of transcription, DNA- dependent|transcription|transcription from RNA polymerase II promoter|zinc ion binding miR- NM_004235 KLF4 Kruppel-like factor 4 P + T mesodermal cell fate determination|negative 10b (gut) regulation of cell proliferation|negative regulation of transcription, DNA-dependent|negative regulation of transcription, DNA-dependent|nucleic acid binding|nucleus|transcription|transcription factor activity|transcription factor activity|transcriptional activator activity|transcriptional activator activity|transcriptional repressor activity|transcriptional repressor activity|zinc ion binding|zinc ion binding miR- AW511293 LOC144455 hypothetical protein P + T regulation of cell cycle|regulation of transcription, 10b BC016658 DNA-dependent|transcription factor activity|transcription factor complex miR- NM_014921 LPHN1 lectomedin-2 P + T G-protein coupled receptor activity|integral to 10b membrane|latrotoxin receptor activity|membrane|neuropeptide signaling pathway|receptor activity|signal transduction|sugar binding miR- NM_012325 MAPRE1 microtubule-associated P + T cell proliferation|cytokinesis|microtubule 10b protein, RP/EB family, binding|mitosis|protein C-terminus binding|regulation member 1 of cell cycle miR- AA824369 MGC4643 hypothetical protein P + T Wnt receptor signaling pathway|endoplasmic 10b MGC4643 reticulum|ligase activity|signal transduction|ubiquitin conjugating enzyme activity|ubiquitin cycle|ubiquitin- dependent protein catabolism miR- NM_021090 MTMR3 myotubularin related P + T cytoplasm|hydrolase activity|inositol or 10b protein 3 phosphatidylinositol phosphatase activity|membrane|membrane fraction|phospholipid dephosphorylation|protein amino acid dephosphorylation|protein serine/threonine phosphatase activity|protein tyrosine phosphatase activity|protein tyrosine/serine/threonine phosphatase activity|zinc ion binding miR- AI498126 NAC1 transcriptional repressor P + T protein binding 10b NAC1 miR- AF128458 NCOA6 nuclear receptor P + T DNA recombination|DNA repair|DNA 10b coactivator 6 replication|brain development|chromatin binding|embryonic development (sensu Mammalia)|estrogen receptor binding|estrogen receptor signaling pathway|glucocorticoid receptor signaling pathway|heart development|ligand- dependent nuclear receptor transcription coactivator activity|myeloid blood cell differentiation|nucleus|nucleus|positive regulation of transcription from RNA polymerase II promoter|protein binding|regulation of transcription, DNA-dependent|response to hormone stimulus|retinoid X receptor binding|thyroid hormone receptor binding|transcription|transcription factor complex|transcription initiation from RNA polymerase II promoter|transcriptional activator activity miR- NM_006312 NCOR2 nuclear receptor co- P + T DNA binding|nucleus|regulation of transcription, 10b repressor 2 DNA-dependent|transcription corepressor activity miR- NM_006599 NFAT5 nuclear factor of P + T RNA polymerase II transcription factor 10b activated T-cells 5, activity|excretion|nucleus|regulation of transcription, tonicity-responsive DNA-dependent|signal transduction|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_006981 NR4A3 nuclear receptor M + P + T binding|nucleus|nucleus|regulation of transcription, 10b subfamily 4, group A, DNA-dependent|steroid hormone receptor member 3 activity|steroid hormone receptor activity|thyroid hormone receptor activity|transcription|transcription factor activity miR- NM_003822 NR5A2 nuclear receptor P + T RNA polymerase II transcription factor activity, 10b subfamily 5, group A, enhancer member 2 binding|morphogenesis|nucleus|nucleus|regulation of transcription, DNA-dependent|steroid hormone receptor activity|transcription|transcription factor activity|transcription from RNA polymerase II promoter miR- AA295257 NRP2 neuropilin 2 P + T angiogenesis|axon guidance|cell adhesion|cell 10b adhesion|cell differentiation|electron transport|electron transporter activity|integral to membrane|integral to membrane|membrane|membrane fraction|neurogenesis|receptor activity|semaphorin receptor activity|vascular endothelial growth factor receptor activity|vascular endothelial growth factor receptor activity miR- NM_000430 PAFAH1B1 platelet-activating P + T astral microtubule|cell cortex|cell cycle|cell 10b factor acetylhydrolase, differentiation|cell isoform Ib, alpha motility|cytokinesis|cytoskeleton|dynein subunit 45 kDa binding|establishment of mitotic spindle orientation|kinetochore|lipid metabolism|microtubule associated complex|microtubule-based process|mitosis|neurogenesis|nuclear membrane|signal transduction miR- NM_013382 POMT2 putative protein O- P + T O-linked glycosylation|dolichyl-phosphate-mannose- 10b mannosyltransferase protein mannosyltransferase activity|endoplasmic reticulum|integral to membrane|magnesium ion binding|membrane|transferase activity, transferring glycosyl groups miR- BF337790 PURB purine-rich element P + T 10b binding protein B miR- AI302106 RAP2A RAP2A, member of P + T GTP binding|GTPase activity|membrane|signal 10b RAS oncogene family transduction|small GTPase mediated signal transduction miR- NM_002886 RAP2B RAP2B, member of P + T GTP binding|protein transport|small GTPase mediated 10b RAS oncogene family signal transduction miR- NM_014781 RB1CC1 RB1-inducible coiled- P + T kinase activity 10b coil 1 miR- NM_012234 RYBP RING1 and YY1 P + T development|negative regulation of transcription from 10b binding protein RNA polymerase II promoter|nucleus|transcription corepressor activity miR- NM_005506 SCARB2 scavenger receptor class P + T cell adhesion|integral to plasma membrane|lysosomal 10b B, member 2 membrane|membrane fraction|receptor activity miR- AF225986 SCN3A sodium channel, P + T cation channel activity|cation transport|integral to 10b voltage-gated, type III, membrane|membrane|sodium ion transport|voltage- alpha polypeptide gated sodium channel activity|voltage-gated sodium channel complex miR- NM_002997 SDC1 syndecan 1 P + T cytoskeletal protein binding|integral to plasma 10b membrane|membrane miR- NM_006924 SFRS1 splicing factor, P + T RNA binding|mRNA splice site selection|nuclear 10b arginine/serine-rich 1 mRNA splicing, via spliceosome|nucleotide (splicing factor 2, binding|nucleus alternate splicing factor) miR- AI809967 SHC1 SHC (Src homology 2 P + T activation of MAPK|activation of MAPK|intracellular 10b domain containing) signaling cascade|phospholipid binding|phospholipid transforming protein 1 binding|plasma membrane|plasma membrane|positive regulation of cell proliferation|positive regulation of cell proliferation|positive regulation of mitosis|positive regulation of mitosis|regulation of cell growth|regulation of epidermal growth factor receptor activity|transmembrane receptor protein tyrosine kinase adaptor protein activity|transmembrane receptor protein tyrosine kinase adaptor protein activity miR- NM_018976 SLC38A solute carrier family 38, P + T amino acid transport|amino acid-polyamine 10b member 2 transporter activity|integral to membrane|membrane|oxygen transport|oxygen transporter activity|transport miR- NM_003794 SNX4 sorting nexin 4 P + T endocytosis|intracellular signaling cascade|protein 10b transport miR- NM_003103 SON SON DNA binding P + T DNA binding|DNA binding|anti-apoptosis|double- 10b protein stranded RNA binding|intracellular|nucleic acid binding|nucleus miR- Z48199 syndecan-1 P + T 10b miR- NM_003222 TFAP2C transcription factor AP- P + T cell-cell signaling|nucleus|regulation of transcription 10b 2 gamma (activating from RNA polymerase II enhancer binding promoter|transcription|transcription factor activity protein 2 gamma) miR- NM_003275 TMOD1 tropomodulin P + T actin binding|cytoskeleton|cytoskeleton organization 10b and biogenesis|tropomyosin binding miR- NM_003367 USF2 upstream transcription P + T RNA polymerase II transcription factor 10b factor 2, c-fos activity|nucleus|regulation of transcription, DNA- interacting dependent|transcription|transcription factor activity miR- N62196 ZNF367 zinc finger protein 367 P + T nucleic acid binding|nucleus|zinc ion binding 10b miR- AI948503 ABCC4 ATP-binding cassette, P + T 15-hydroxyprostaglandin dehydrogenase (NAD+) 125b sub-family C activity|ATP binding|ATPase activity|ATPase (CFTR/MRP), member 4 activity, coupled to transmembrane movement of substances|chloride channel activity|integral to membrane|ion transport|membrane miR- AL534702 ABHD3 abhydrolase domain M + P + T 125b containing 3 miR- AL527773 ABR active BCR-related P + T GTPase activator activity|guanyl-nucleotide exchange 125b gene factor activity|small GTPase mediated signal transduction miR- NM_020039 ACCN2 amiloride-sensitive P + T amiloride-sensitive sodium channel activity|integral to 125b cation channel 2, plasma membrane|ion channel activity|ion neuronal transport|membrane|response to pH|signal transduction|sodium ion transport miR- NM_003816 ADAM9 a disintegrin and P + T SH3 domain binding|integral to plasma 125b metalloproteinase membrane|integrin binding|metalloendopeptidase domain 9 (meltrin activity|protein binding|protein kinase binding|protein gamma) kinase cascade|proteolysis and peptidolysis|zinc ion binding miR- L05500 ADCY1 adenylate cyclase 1 P + T cAMP biosynthesis|calcium- and calmodulin- 125b (brain) responsive adenylate cyclase activity|calmodulin binding|integral to membrane|intracellular signaling cascade|magnesium ion binding miR- NM_017488 ADD2 adducin 2 (beta) P + T actin binding|actin cytoskeleton|calmodulin 125b binding|membrane miR- NM_003488 AKAP1 A kinase (PRKA) P + T RNA binding|integral to 125b anchor protein 1 membrane|mitochondrion|outer membrane miR- NM_005465 AKT3 v-akt murine thymoma P + T ATP binding|protein amino acid 125b viral oncogene homolog phosphorylation|protein serine/threonine kinase 3 (protein kinase B, activity|signal transduction|transferase activity gamma) miR- NM_001150 ANPEP alanyl (membrane) P + T aminopeptidase activity|angiogenesis|cell 125b aminopeptidase differentiation|integral to plasma (aminopeptidase N, membrane|membrane alanyl aminopeptidase
aminopeptidase M, activity|metallopeptidase activity|proteolysis and microsomal peptidolysis|receptor activity|zinc ion binding aminopeptidase, CD13, p150) miR- AF193759 APBA2BP amyloid beta (A4) M + P + T Golgi cis cisterna|Golgi cis cisterna|antibiotic 125b precursor protein- biosynthesis|calcium ion binding, family A, binding|cytoplasm|cytoplasm|endoplasmic reticulum member 2 binding membrane|endoplasmic reticulum protein membrane|nucleus|oxidoreductase activity|protein binding|protein binding|protein binding|protein metabolism|protein metabolism|protein secretion|protein secretion|regulation of amyloid precursor protein biosynthesis miR- NM_000038 APC adenomatosis polyposis P + T Wnt receptor signaling pathway|beta-catenin 125b coli binding|cell adhesion|microtubule binding|negative regulation of cell cycle|protein complex assembly|signal transduction miR- NM_001655 ARCN1 archain 1 P + T COPI vesicle coat|Golgi apparatus|clathrin vesicle 125b coat|intra-Golgi transport|intracellular protein transport|intracellular protein transport|membrane|retrograde transport, Golgi to ER|transport miR- BC001719 ASB6 ankyrin repeat and M + P intracellular signaling cascade 125b SOCS box-containing 6 miR- AI478147 ATP10D ATPase, Class V, type P + T ATP binding|ATPase activity|cation 125b 10D transport|hydrolase activity|integral to membrane|magnesium ion binding|membrane|phospholipid-translocating ATPase activity miR- NM_012069 ATP1B4 ATPase, (Na+)/K+ P + T hydrogen ion transporter activity|integral to plasma 125b transporting, beta 4 membrane|ion transport|membrane|potassium ion polypeptide transport|proton transport|sodium ion transport|sodium:potassium-exchanging ATPase activity miR- NM_005176 ATP5G2 ATP synthase, H+ M + P + T ATP synthesis coupled proton transport|hydrogen- 125b transporting, transporting ATP synthase activity, rotational mitochondrial F0 mechanism|hydrogen-transporting ATPase activity, complex, subunit c rotational mechanismlion transport|lipid (subunit 9), isoform 2 binding|membrane|membrane fraction|mitochondrion|proton transport|proton- transporting ATP synthase complex (sensu Eukaryota)|proton-transporting two-sector ATPase complex|transporter activity miR- NM_001702 BAI1 brain-specific M + P + T G-protein coupled receptor 125b angiogenesis inhibitor 1 activity|axonogenesis|brain-specific angiogenesis inhibitor activity|cell adhesion|integral to plasma membrane|intercellular junction|negative regulation of cell proliferation|neuropeptide signaling pathway|peripheral nervous system development|plasma membrane|protein binding|receptor activity|signal transduction miR- NM_001188 BAK1 BCL2-antagonist/killer 1 M + T apoptotic mitochondrial changes|induction of 125b apoptosis|integral to membrane|protein heterodimerization activity|regulation of apoptosis miR- NM_013449 BAZ2A bromodomain adjacent P + T DNA binding|chromatin remodeling|nucleolus 125b to zinc finger domain, organizer complex|nucleus|regulation of transcription, 2A DNA-dependent|transcription|transcription regulator activity miR- NM_004634 BRPF1 bromodomain and PHD M + P + T DNA binding|nucleus|nucleus|regulation of 125b finger containing, 1 transcription, DNA-dependent|transcription|zinc ion binding miR- NM_003458 BSN bassoon (presynaptic P + T cytoskeleton|metal ion binding|nucleus|structural 125b cytomatrix protein) constituent of cytoskeleton|synapse|synaptic transmission|synaptosome miR- NM_018108 C14orf130 hypothetical protein P + T ubiquitin cycle|ubiquitin-protein ligase activity 125b FLJ10483 miR- AA025877 C20orf136 chromosome 20 open P + T 125b reading frame 136 miR- AB054985 CACNB1 calcium channel, M + P + T calcium ion transport|ion transport|membrane 125b voltage-dependent, beta fraction|muscle contraction|voltage-gated calcium 1 subunit channel activity|voltage-gated calcium channel complex miR- NM_001224 CASP2 caspase 2, apoptosis- P + T anti-apoptosis|apoptotic program|caspase 125b related cysteine activity|caspase activity|caspase activity|cysteine-type protease (neural peptidase activity|enzyme binding|intracellular|protein precursor cell binding|proteolysis and peptidolysis|proteolysis and expressed, peptidolysis|regulation of apoptosis developmentally down- regulated 2) miR- NM_001755 CBFB core-binding factor, M + P + T RNA polymerase II transcription factor 125b beta subunit activity|nucleus|transcription coactivator activity|transcription factor activity|transcription from RNA polymerase II promoter miR- AV648364 CBX7 ESTs, Highly similar to P + T chromatin|chromatin assembly or 125b potassium voltage-gated disassembly|chromatin binding|chromatin channel, Isk-related modification|nucleus|regulation of transcription, subfamily, gene 4; DNA-dependent|transcription potassium voltage-gated channel-like protein, Isk-related subfamily [Homo sapiens] [H. sapiens] miR- NM_001408 CELSR2 cadherin, EGF LAG M + P + T G-protein coupled receptor activity|calcium ion 125b seven-pass G-type binding|cell adhesion|development|homophilic cell receptor 2 (flamingo adhesion|integral to homolog, Drosophila) membrane|membrane|neuropeptide signaling pathway|receptor activity|signal transduction|structural molecule activity miR- NM_015955 CGI-27 C21orf19-like protein P + T 125b miR- AF263462 CGN cingulin P + T actin binding|biological_process unknown|motor 125b activity|myosin|protein binding|tight junction miR- AF064491 CLIM2 LIM domain binding 1 P + T LIM domain 125b binding|development|development|negative regulation of transcription, DNA-dependent|nucleus|transcription cofactor activity|transcriptional repressor activity miR- AU152178 CMG2 capillary P + T integral to membrane|receptor activity 125b morphogenesis protein 2 miR- NM_004073 CNK cytokine-inducible P + T ATP binding|protein amino acid 125b kinase phosphorylation|protein binding|protein serine/threonine kinase activity|regulation of cell cycle|transferase activity miR- NM_020348 CNNM1 cyclin M1 M + P + T fatty acid biosynthesis 125b miR- NM_022730 COPS7B COP9 constitutive M + P + T signalosome complex 125b photomorphogenic homolog subunit 7B (Arabidopsis) miR- NM_003389 CORO2A coronin, actin binding P + T actin binding|glutamate-ammonia ligase 125b protein, 2A activity|glutamine biosynthesis|intracellular signaling cascade|nitrogen compound metabolism|protein binding miR- BF939649 CORO2B coronin, actin binding P + T actin binding|actin cytoskeleton|actin cytoskeleton 125b protein, 2B organization and biogenesis|membrane miR- NM_007007 CPSF6 cleavage and P + T RNA binding|mRNA processing|nucleic acid 125b polyadenylation binding|nucleotide binding|nucleus specific factor 6, 68 kDa miR- NM_004386 CSPG3 chondroitin sulfate P + T calcium ion binding|cell adhesion|cell 125b proteoglycan 3 motility|hyaluronic acid binding|sugar binding (neurocan) miR- NM_004393 DAG1 dystroglycan 1 M + P + T actin cytoskeleton|calcium ion binding|extracellular 125b (dystrophin-associated matrix (sensu Metazoa)|integral to plasma glycoprotein 1) membrane|laminin receptor activity|membrane fraction|muscle contraction|plasma membrane|protein binding|protein complex assembly miR- NM_014764 DAZAP2 DAZ associated protein 2 P + T 125b miR- NM_030927 DC- tetraspanin similar to P + T integral to membrane 125b TM4F2 TM4SF9 miR- NM_004082 DCTN1 dynactin 1 (p150, glued M + P + T cytoplasm|cytoskeleton|dynein complex|mitosis|motor 125b homolog, Drosophila) activity|neurogenesis miR- NM_030621 DICER1 Dicer1, Dcr-1 homolog P + T ATP binding|ATP-dependent helicase activity|RNA 125b (Drosophila) interference, targeting of mRNA for destruction|RNA processing|double-stranded RNA binding|endonuclease activity|hydrolase activity|intracellular|ribonuclease III activity miR- U53506 DIO2 deiodinase, P + T integral to membrane|membrane|selenium 125b iodothyronine, type II binding|selenocysteine incorporation|thyroid hormone generation|thyroxine 5'-deiodinase activity|thyroxine 5'-deiodinase activity miR- AL136139 dJ761I2.1 P + T 125b miR- AL357503 dJ899C14.1 Q9H4T4 like P + T 125b miR- AL117482 DKFZP434C131 DKFZP434C131 P + T ATP binding|protein amino acid 125b protein phosphorylation|protein serine/threonine kinase activity|protein-tyrosine kinase activity|transferase activity miR- AK023580 DKFZP434H0820 hypothetical protein P + T 125b DKFZp434H0820 miR- T16388 DKFZp564A176 hypothetical protein P + T development|integral to membrane|membrane|receptor 125b DKFZp564A176 activity|semaphorin receptor activity miR- AL137517 DKFZp564O1278 hypothetical protein P + T integral to membrane 125b DKFZp564O1278 miR- BE781961 DKFZp762A2013 hypothetical protein P + T electron transport|electron transporter activity 125b DKFZp762A2013 miR- AB036931 DLL4 delta-like 4 M + P + T Notch binding|Notch signaling pathway|cell 125b (Drosophila) differentiation|circulation|integral to membrane|membrane|signal transduction miR- NM_012266 DNAJB5 DnaJ (Hsp40) homolog, P + T heat shock protein binding|protein folding|response to 125b subfamily B, member 5 unfolded protein|unfolded protein binding miR- NM_005740 DNAL4 dynein, axonemal, light P + T ATPase activity, coupled|axonemal dynein 125b polypeptide 4 complex|microtubule motor activity|microtubule- based movement miR- BF593175 DOCK3 dedicator of cyto- P + T GTP binding|GTPase binding|guanyl-nucleotide 125b kinesis 3 exchange factor activity miR- NM_006426 DPYSL4 dihydropyrimidinase- P + T hydrolase activity|neurogenesis 125b like 4 miR- NM_006465 DRIL2 dead ringer P + T DNA binding|biological_process unknown|nucleus 125b (Drosophila)-like 2 (bright and dead ringer) miR- BC005047 DUSP6 dual specificity P + T MAP kinase phosphatase activity|cytoplasm|hydrolase 125b phosphatase 6 activity|inactivation of MAPK|protein amino acid dephosphorylation|protein serine/threonine phosphatase activity|protein tyrosine phosphatase activity|regulation of cell cycle|soluble fraction miR- NM_004423 DVL3 dishevelled, dsh P + T development|frizzled signaling pathway|heart 125b homolog 3 (Drosophila) development|intracellular|intracellular signaling cascade|kinase activity|neurogenesis|protein binding|signal transducer activity miR- NM_001949 E2F3 E2F transcription factor 3 P + T nucleus|protein binding|regulation of cell 125b cycle|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcription factor complex|transcription initiation from RNA polymerase II promoter miR- AU149385 EAF1 Homo sapiens cDNA P + T 125b FLJ13155 fis, clone NT2RP3003433, mRNA sequence miR- NM_014674 EDEM KIAA0212 gene P + T ER-associated protein
catabolism|GTP binding|N- 125b product linked glycosylation|calcium ion binding|endoplasmic reticulum|integral to endoplasmic reticulum membrane|integral to membrane|mannosyl- oligosaccharide 1,2-alpha-mannosidase activity|membrane|protein binding|response to unfolded protein miR- NM_001955 EDN1 endothelin 1 M + P + T cell-cell signaling|extracellular space|hormone 125b activity|pathogenesis|positive regulation of cell proliferation|regulation of blood pressure|regulation of vasoconstriction|signal transduction|soluble fraction miR- AI832074 EIF2C2 eukaryotic translation M + P cellular_component unknown|protein 125b initiation factor 2C, 2 biosynthesis|translation initiation factor activity miR- AB044548 EIF4EBP1 eukaryotic translation P + T eukaryotic initiation factor 4E binding|negative 125b initiation factor 4E regulation of protein biosynthesis|negative regulation binding protein 1 of translational initiation|regulation of translation miR- NM_020390 EIF5A2 eukaryotic translation P + T DNA binding|protein biosynthesis|translation 125b initiation factor 5A2 initiation factor activity|translational initiation miR- NM_004438 EPHA4 EphA4 P + T ATP binding|ephrin receptor activity|integral to 125b plasma membrane|membrane|protein amino acid phosphorylation|receptor activity|signal transduction|transferase activity|transmembrane receptor protein tyrosine kinase signaling pathway miR- NM_004451 ESRRA estrogen-related P + T nucleus|regulation of transcription, DNA- 125b receptor alpha dependent|steroid binding|steroid hormone receptor activity|transcription|transcription factor activity miR- NM_004907 ETR101 immediate early protein P + T 125b miR- NM_005238 ETS1 v-ets erythroblastosis P + T RNA polymerase II transcription factor 125b virus E26 oncogene activity|immune response|negative regulation of cell homolog 1 (avian) proliferation|nucleus|regulation of transcription, DNA-dependent|transcription|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_001987 ETV6 ets variant gene 6 (TEL P + T nucleus|regulation of transcription, DNA- 125b oncogene) dependent|transcription|transcription factor activity miR- NM_022763 FAD104 FAD104 P + T 125b miR- AF308300 FAPP2 phosphoinositol 4- P + T 125b phosphate adaptor protein-2 miR- NM_022976 FGFR2 fibroblast growth factor M + P + T ATP binding|cell growth|fibroblast growth factor 125b receptor 2 (bacteria- receptor activity|heparin binding|integral to expressed kinase, membrane|membrane|protein amino acid keratinocyte growth phosphorylation|protein amino acid factor receptor, phosphorylation|protein serine/threonine kinase craniofacial dysostosis activity|protein-tyrosine kinase activity|protein- 1, Crouzon syndrome, tyrosine kinase activity|receptor activity|transferase Pfeiffer syndrome, activity Jackson-Weiss syndrome) miR- NM_004470 FKBP2 FK506 binding protein P + T FK506 binding|endoplasmic reticulum|isomerase 125b 2, 13 kDa activity|peptidyl-prolyl cis-trans isomerase activity|protein folding miR- AL160175 FKHL18 forkhead-like 18 P + T 125b (Drosophila) miR- BF515132 FLJ00024 hypothetical protein P + T 125b FLJ00024 miR- BC002945 FLJ10101 hypothetical protein M + P GTP binding|protein transport|small GTPase mediated 125b FLJ10101 signal transduction miR- NM_018243 FLJ10849 hypothetical protein P + T GTP binding|cell cycle|cytokinesis 125b FLJ10849 miR- NM_019084 FLJ10895 hypothetical protein P + T nucleus|regulation of cell cycle 125b FLJ10895 miR- NM_018320 FLJ11099 hypothetical protein P + T protein ubiquitination|ubiquitin ligase 125b FLJ11099 complex|ubiquitin-protein ligase activity|zinc ion binding miR- NM_018375 FLJ11274 hypothetical protein M + P + T membrane|metal ion transport|metal ion transporter 125b FLJ11274 activity miR- NM_024954 FLJ11807 hypothetical protein P + T protein modification 125b FLJ11807 miR- BF434995 FLJ14708 hypothetical protein P + T 125b FLJ14708 miR- NM_018992 FLJ20040 hypothetical protein P + T membrane|potassium ion transport|protein 125b FLJ20040 binding|voltage-gated potassium channel activity|voltage-gated potassium channel complex miR- NM_017911 FLJ20635 hypothetical protein P + T 125b FLJ20635 miR- NM_017936 FLJ2070 hypothetical protein M + P + T ATP synthesis coupled proton 125b FLJ20707 transport|cytoplasm|hydrogen-transporting ATP synthase activity, rotational mechanism|hydrogen- transporting ATPase activity, rotational mechanism|membrane|phosphate transport|proton- transporting two-sector ATPase complex miR- NM_024789 FLJ22529 hypothetical protein P + T 125b FLJ22529 miR- AA721230 FLJ25604 hypothetical protein P + T guanyl-nucleotide exchange factor activity|small 125b FLJ25604 GTPase mediated signal transduction miR- AI677701 FLJ30829 hypothetical protein P + T nucleic acid binding|nucleotide binding 125b FLJ30829 miR- NM_004475 FLOT2 flotillin 2 M + P + T cell adhesion|epidermis development|flotillin 125b complex|integral to membrane|plasma membrane|protein binding miR- AA830884 FMR1 fragile X mental M + T mRNA binding|mRNA processing|mRNA-nucleus 125b retardation 1 export|nucleoplasm|polysome|ribosome|soluble fraction|transport miR- AF305083 FUT4 fucosyltransferase 4 P + T Golgi apparatus|L-fucose catabolism|alpha(1,3)- 125b (alpha (1,3) fucosyltransferase activity|carbohydrate fucosyltransferase, metabolism|integral to myeloid-specific) membrane|membrane|membrane fraction|protein amino acid glycosylation|transferase activity, transferring glycosyl groups miR- X92762 G4.5 tafazzin M + P + T acyltransferase activity|heart development|integral to 125b (cardiomyopathy, membrane|metabolism|muscle contraction|muscle dilated 3A (X-linked); development endocardial fibroelastosis 2; Barth syndrome) miR- NM_012296 GAB2 GRB2-associated P + T 125b binding protein 2 miR- NM_015044 GGA2 golgi associated, M + T ADP-ribosylation factor binding|Golgi stack|Golgi 125b gamma adaptin ear trans face|clathrin coat of trans-Golgi network containing, ARF vesicle|intra-Golgi transport|intracellular protein binding protein 2 transport|intracellular protein transport|membrane|protein complex assembly|protein transporter activity miR- AL049709 GGTL3 gamma- M + P + T 125b glutamyltransferase-like 3 miR- NM_000165 GJA1 gap junction protein, P + T cell-cell signaling|connexon channel 125b alpha 1, 43 kDa activity|connexon complex|gap junction (connexin 43) assembly|heart development|integral to plasma membrane|ion transporter activity|muscle contraction|perception of sound|positive regulation of I-kappaB kinase/NF-kappaB cascade|protein binding|signal transducer activity|transport miR- NM_014905 GLS glutaminase P + T glutaminase activity|glutamine catabolism|hydrolase 125b activity|mitochondrion miR- NM_005113 GOLGA5 golgi autoantigen, P + T ATP binding|Golgi membrane|cell surface receptor 125b golgin subfamily a, 5 linked signal transduction|integral to plasma membrane|protein amino acid phosphorylation|protein-tyrosine kinase activity miR- NM_001448 GPC4 glypican 4 M + P + T cell proliferation|extracellular matrix (sensu 125b Metazoa)|integral to plasma membrane|membrane|morphogenesis miR- NM_005296 GPR23 G protein-coupled M + T G-protein coupled receptor protein signaling 125b receptor 23 pathway|integral to plasma membrane|purinergic nucleotide receptor activity, G-protein coupled|receptor activity|rhodopsin-like receptor activity|signal transduction miR- U66065 GRB10 growth factor receptor- M + T SH3/SH2 adaptor activity|cell-cell 125b bound protein 10 signaling|cytoplasm|insulin receptor signaling pathway|intracellular signaling cascade|plasma membrane miR- NM_021643 GS3955 GS3955 protein P + T ATP binding|protein amino acid 125b phosphorylation|protein kinase activity|transferase activity miR- NM_019096 GTPBP2 GTP binding protein 2 M + T GTP binding|GTPase activity|protein 125b biosynthesis|small GTPase mediated signal transduction miR- U78181 hBNaC2 amiloride-sensitive P + T amiloride-sensitive sodium channel activity|integral to 125b cation channel 2, plasma membrane|ion channel activity|ion neuronal transport|membrane|response to pH|signal transduction|sodium ion transport miR- NM_005477 HCN4 hyperpolarization P + T 3',5'-cAMP binding|cation channel activity|cation 125b activated cyclic transport|circulation|integral to plasma nucleotide-gated membrane|membrane|membrane fraction|muscle potassium channel 4 contraction|nucleotide binding|potassium ion transport|sodium ion transport|voltage-gated potassium channel activity miR- NM_002112 HDC histidine decarboxylase P + T amino acid metabolism|catecholamine 125b biosynthesis|histidine decarboxylase activity|histidine metabolism|lyase activity miR- U64317 HEF1 enhancer of P + T actin filament bundle formation|cell adhesion| 125b filamentation 1 (cas-like cytokinesis|cytoplasm|cytoskeleton|cytoskeleton docking; Crk-associated organization and biogenesis|integrin-mediated substrate related) signaling pathway|mitosis|nucleus|protein binding|regulation of cell cycle|regulation of cell growth|signal transduction|spindle miR- L38487 hERRa estrogen-related P + T nucleus|regulation of transcription, DNA- 125b receptor alpha dependent|steroid binding|steroid hormone receptor activity|transcription|transcription factor activity miR- AB028943 HIC2 hypermethylated in P + T DNA binding|negative regulation of transcription, 125b cancer 2 DNA-dependent|nucleus|protein C-terminus binding|transcription|zinc ion binding miR- AL023584 HIVEP2 human P + T 125b immunodeficiency virus type I enhancer binding protein 2 miR- AL023584 HIVEP2 human P + T 125b immunodeficiency virus type I enhancer binding protein 2 miR- NM_005342 HMGB3 high-mobility group P + T DNA bending activity|DNA 125b box 3 binding|chromatin|development|nucleus|regulation of transcription, DNA-dependent miR- AL031295 HMGCL; lysophospholipase II M + P + T 125b HL miR- NM_004503 HOXC6 homeo box C6 P + T development|development|nucleus|regulation of 125b transcription from RNA polymerase II promoter|regulation of transcription, DNA- dependent|transcription corepressor activity|transcription factor activity miR- AA844682 HRD1 HRD1 protein P + T protein ubiquitination|ubiquitin ligase 125b complex|ubiquitin-protein ligase activity|zinc ion binding miR- AL136667 HSPC039 HSPC039 protein P + T integral to membrane 125b miR- AF245044 HT023 hypothetical protein P + T 125b HT023 miR- U13022 Ich-1 caspase 2, apoptosis- P + T anti-apoptosis|apoptotic program|caspase 125b related cysteine activity|caspase activity|caspase
activity|cysteine-type protease (neural peptidase activity|enzyme binding|intracellular|protein precursor cell binding|proteolysis and peptidolysis|proteolysis and expressed, peptidolysis|regulation of apoptosis developmentally down- regulated 2) miR- NM_004513 IL16 interleukin 16 M + P + T chemotaxis|cytokine activity|extracellular 125b (lymphocyte space|immune response|protein binding|sensory chemoattractant factor) perception miR- NM_002460 IRF4 interferon regulatory P + T RNA polymerase II transcription factor activity|T-cell 125b factor 4 activation|T-cell activation|nucleus|nucleus|nucleus|positive regulation of interleukin-10 biosynthesis|positive regulation of interleukin-10 biosynthesis|positive regulation of interleukin-13 biosynthesis|positive regulation of interleukin-13 biosynthesis|positive regulation of interleukin-2 biosynthesis|positive regulation of interleukin-2 biosynthesis|positive regulation of interleukin-4 biosynthesis|positive regulation of interleukin-4 biosynthesis|positive regulation of transcription|positive regulation of transcription|regulation of T-helper cell differentiation|regulation of T-helper cell differentiation|regulation of transcription, DNA- dependent|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcription factor activity|transcription factor binding|transcription factor binding|transcriptional activator activity|transcriptional activator activity miR- NM_002207 ITGA9 integrin, alpha 9 P + T cell-matrix adhesion|integral to membrane|integrin 125b complex|integrin-mediated signaling pathway|protein binding|receptor activity miR- NM_000212 ITGB3 integrin, beta 3 (platelet P + T blood coagulation|cell-matrix adhesion|integrin 125b glycoprotein IIIa, complex|integrin-mediated signaling pathway|protein antigen CD61) binding|receptor activity miR- NM_021991 JUP junction plakoglobin P + T cell adhesion|cell adhesion|cytoplasm|cytoskeletal 125b protein binding|cytoskeleton|cytoskeleton|membrane fraction|mitotic chromosome condensation|protein binding|soluble fraction|structural molecule activity miR- AF032897 KCNH7 potassium voltage-gated P + T cation transport|integral to 125b channel, subfamily H membrane|membrane|potassium ion (eag-related), member 7 transport|regulation of transcription, DNA- dependent|signal transducer activity|signal transduction|voltage-gated potassium channel activity miR- NM_002252 KCNS3 potassium voltage-gated M + P + T cation transport|delayed rectifier potassium channel 125b channel, delayed- activity|membrane|membrane fraction|potassium rectifier, subfamily S, channel regulator activity|potassium ion member 3 transport|protein binding|voltage-gated potassium channel complex miR- NM_014735 KIAA0215 KIAA0215 gene P + T DNA binding|regulation of transcription, DNA- 125b product dependent miR- NM_015288 KIAA0239 KIAA0239 protein P + T DNA binding|regulation of transcription, DNA- 125b dependent miR- D87469 KIAA0279 cadherin, EGF LAG M + P + T G-protein coupled receptor activity|calcium ion 125b seven-pass G-type binding|cell adhesion|development|homophilic cell receptor 2 (flamingo adhesion|integral to homolog, Drosophila) membrane|membrane|neuropeptide signaling pathway|receptor activity|signal transduction|structural molecule activity miR- AB002356 KIAA0358 MAP-kinase activating P + T cell surface receptor linked signal 125b death domain transduction|cytoplasm|death receptor binding|kinase activity|plasma membrane|protein kinase activator activity miR- NM_014871 KIAA0710 KIAA0710 gene P + T cysteine-type endopeptidase activity|exonuclease 125b product activity|nucleus|ubiquitin cycle|ubiquitin thiolesterase activity|ubiquitin-dependent protein catabolism miR- AB 018333 KIAA0790 KIAA0790 protein P + T cell cycle|negative regulation of cell cycle 125b miR- NM_014912 KIAA0940 KIAA0940 protein P + T nucleic acid binding 125b miR- AB028957 KIAA1034 KIAA1034 protein P + T DNA binding|nucleus|regulation of transcription, 125b DNA-dependent|transcription factor activity miR- NM_014901 KIAA1100 KIAA1100 protein M + P + T protein ubiquitination|ubiquitin ligase 125b complex|ubiquitin-protein ligase activity|zinc ion binding miR- AB033016 KIAA1190 hypothetical protein P + T DNA binding|nucleic acid binding|nucleus|protein 125b KIAA1190 binding|regulation of transcription, DNA- dependent|zinc ion binding miR- AA056548 KIAA1268 KIAA1268 protein P + T NAD + ADP-ribosyltransferase 125b activity|nucleus|protein amino acid ADP-ribosylation miR- BE670098 KIAA1594 KIAA1594 protein M + P + T cysteine-type endopeptidase activity|ubiquitin 125b cycle|ubiquitin thiolesterase activity|ubiquitin- dependent protein catabolism miR- AU157109 KIAA1598 KIAA1598 protein P + T 125b miR- AA772278 KIAA1673 KIAA1673 P + T 125b miR- NM_015995 KLF13 Kruppel-like factor 13 P + T DNA binding|RNA polymerase II transcription factor 125b activity|nucleus|regulation of transcription, DNA- dependent|transcription|transcription from RNA polymerase II promoter|zinc ion binding miR- NM_016531 KLF3 Kruppel-like factor 3 P + T development|negative regulation of transcription from 125b (basic) RNA polymerase II promoter|nucleus|regulation of transcription, DNA- dependent|transcription|transcription factor activity|zinc ion binding miR- BE892574 LACTB lactamase, beta P + T hydrolase activity|integral to membrane|response to 125b antibiotic miR- BE566136 LBP-32 LBP protein 32 P + T 125b miR- NM_024090 LCE long-chain fatty-acyl P + T integral to membrane 125b elongase miR- NM_003893 LDB1 LIM domain binding 1 P + T LIM domain 125b binding|development|development|negative regulation of transcription, DNA-dependent|nucleus|transcription cofactor activity|transcriptional repressor activity miR- U94354 LFNG lunatic fringe homolog M + T Golgi apparatus|development|extracellular 125b (Drosophila) region|integral to membrane|membrane|organogenesis|transferase activity, transferring glycosyl groups miR- NM_002310 LIFR leukemia inhibitory M + P + T cell surface receptor linked signal 125b factor receptor transduction|integral to plasma membrane|leukemia inhibitory factor receptor activity|membrane|receptor activity miR- NM_016339 Link- Link guanine nucleotide P + T G-protein coupled receptor protein signaling 125b GEFII exchange factor II pathway|guanyl-nucleotide exchange factor activity|membrane fraction|neurogenesis|small GTPase mediated signal transduction miR- NM_005575 LNPEP leucyl/cystinyl P + T aminopeptidase activity|cell-cell signaling|integral to 125b aminopeptidase plasma membrane|membrane alanyl aminopeptidase activity|metallopeptidase activity|plasma membrane|pregnancy|proteolysis and peptidolysis|zinc ion binding miR- AL031186 LOC129080 putative emu1 P + T 125b miR- AI884701 LOC221002 CG4853 gene product M + P guanyl-nucleotide exchange factor activity|small 125b GTPase mediated signal transduction miR- AI953847 LOC255488 Homo sapiens mRNA P + T electron transport|electron transporter activity|integral 125b full length insert cDNA to membrane|iron ion binding|ligase activity|protein clone EUROIMAGE binding|protein ubiquitination during ubiquitin- 186647, mRNA dependent protein catabolism|ubiquitin ligase sequence complex|ubiquitin-protein ligase activity|zinc ion binding miR- NM_015899 LOC51054 putative glycolipid P + T 125b transfer protein miR- AA209239 LOC57406 lipase protein P + T aromatic compound metabolism|hydrolase 125b activity|response to toxin|xenobiotic metabolism miR- NM_005576 LOXL1 lysyl oxidase-like 1 M + P + T copper ion binding|electron transporter 125b activity|extracellular region|oxidoreductase activity|protein modification|protein-lysine 6-oxidase activity miR- AA584297 LRP4 low density lipoprotein M + T calcium ion binding|endocytosis|integral to 125b receptor-related protein 4 membrane|membrane|receptor activity miR- NM_007260 LYPLA2 lysophospholipase II M + P + T fatty acid metabolism|hydrolase activity|lipid 125b metabolism miR- NM_004901 LYSAL1 lysosomal apyrase-like 1 P + T Golgi apparatus|UDP catabolism|apyrase 125b activity|hydrolase activity|integral to Golgi membrane|integral to membrane|lysosome|magnesium ion binding|nucleobase, nucleoside, nucleotide and nucleic acid metabolism|uridine-diphosphatase activity|vacuolar membrane miR- NM_002355 M6PR mannose-6-phosphate M + P + T endosome to lysosome transport|integral to plasma 125b receptor (cation membrane|lysosome|receptor mediated dependent) endocytosis|transmembrane receptor activity|transport|transporter activity miR- AB002356 MADD MAP-kinase activating P + T cell surface receptor linked signal 125b death domain transduction|cytoplasm|death receptor binding|kinase activity|plasma membrane|protein kinase activator activity miR- NM_016219 MAN1B1 mannosidase, alpha, P + T N-linked glycosylation|N-linked 125b class 1B, member 1 glycosylation|calcium ion binding|calcium ion binding|carbohydrate metabolism|endoplasmic reticulum|hydrolase activity, acting on glycosyl bonds|integral to membrane|mannosyl- oligosaccharide 1,2-alpha-mannosidase activity|mannosyl-oligosaccharide 1,2-alpha- mannosidase activity|membrane|membrane fraction|oligosaccharide metabolism miR- NM_002446 MAP3K10 mitogen-activated P + T ATP binding|JUN kinase kinase kinase 125b protein kinase kinase activity|activation of kinase 10 JNK|autophosphorylation|induction of apoptosis|protein homodimerization activity|protein serine/threonine kinase activity|protein-tyrosine kinase activity|signal transduction|transferase activity miR- NM_002419 MAP3K11 mitogen-activated M + P + T ATP binding|G1 phase of mitotic cell cycle|JUN 125b protein kinase kinase kinase kinase kinase activity|activation of kinase 11 JNK|autophosphorylation|cell proliferation|centrosome|microtubule|microtubule- based process|protein homodimerization activity|protein oligomerization|protein serine/threonine kinase activity|protein-tyrosine kinase activity|transferase activity miR- Z25432 MAPK14 mitogen-activated P + T ATP binding|MAP kinase activity|MAP kinase kinase 125b protein kinase 14 activity|MP kinase activity|antimicrobial humoral response (sensu Vertebrata)|cell motility|cell surface receptor linked signal transduction|chemotaxis|cytoplasm|nucleus|protein amino acid phosphorylation|protein kinase cascade|protein serine/threonine kinase activity|protein-tyrosine kinase activity|response to stress|transferase activity miR- NM_018650 MARK1 MAP/microtubule P + T ATP binding|cytoplasm|cytoskeleton|cytoskeleton 125b affinity-regulating organization and biogenesis|magnesium ion kinase 1 binding|microtubule cytoskeleton|protein amino acid phosphorylation|protein amino acid phosphorylation|protein kinase cascade|protein serine/threonine kinase activity|protein serine/threonine kinase activity|transferase activity miR- NM_001879 MASP1 mannan-binding lectin P + T calcium ion binding|chymotrypsin 125b serine protease 1 activity|complement activation|complement (C4/C2 activating activation, classical pathway|extracellular component of Ra- region|immune response|peptidase activity|proteolysis reactive factor) and peptidolysis|trypsin activity miR- NM_005911 MAT2A methionine P + T ATP binding|magnesium ion
binding|methionine 125b adenosyltransferase II, adenosyltransferase activity|one-carbon compound alpha metabolism|transferase activity miR- NM_005920 MEF2D MADS box P + T muscle development|nucleus|regulation of 125b transcription enhancer transcription, DNA- factor 2, polypeptide D dependent|transcription|transcription coactivator (myocyte enhancer activity|transcription factor activity|transcription from factor 2D) RNA polymerase II promoter miR- NM_020149 MEIS2 Meis1, myeloid M + P negative regulation of transcription from RNA 125b ecotropic viral polymerase II promoter|nucleus|regulation of integration site 1 transcription, DNA-dependent|specific RNA homolog 2 (mouse) polymerase II transcription factor activity|transcription corepressor activity|transcription factor activity|transcription factor activity miR- NM_017927 MEN1 mitofusin 1 P + T GTP binding|GTPase activity|hydrolase 125b activity|integral to membrane|mitochondrial fusion|mitochondrial outer membrane|mitochondrion miR- AI139252 MGC16063 ribosomal protein L35a P + T JAK-STAT cascadelacute-phase response|calcium ion 125b binding|cell motility|cytoplasm|hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity|intracellular signaling cascade|negative regulation of transcription from RNA polymerase II promoter|neurogenesis|nucleus|nucleus|regulation of transcription, DNA-dependent|signal transducer activity|transcription|transcription factor activity|transcription factor activity miR- AI862120 MGC21981 hypothetical protein P + T membrane 125b MGC21981 miR- AL515061 MGC24302 hypothetical protein P + T 125b MGC24302 miR- BE618656 MGC2541 similar to RIKEN M + P + T 125b cDNA 2610030J16 gene miR- BC005842 MGC2705 hypothetical protein P + T 125b MGC2705 miR- NM_024293 MGC3035 hypothetical protein M + P 125b MGC3035 miR- NM_017572 MKNK2 MAP kinase-interacting P + T ATP binding|ATP binding|cell surface receptor linked 125b serine/threonine kinase 2 signal transduction|protein amino acid phosphorylation|protein amino acid phosphorylation|protein kinase cascade|protein serine/threonine kinase activity|protein serine/threonine kinase activity|protein-tyrosine kinase activity|regulation of translation|response to stress|transferase activity miR- NM_005439 MLF2 myeloid leukemia factor 2 P + T defense response|nucleus 125b miR- NM_007359 MLN51 MLN51 protein P + T mRNA processing|mRNA-nucleus 125b export|molecular_function unknown|nucleus|transport miR- NM_002442 MSI1 musashi homolog 1 M + P + T RNA binding|neurogenesis|nucleotide binding|nucleus 125b (Drosophila) miR- NM_021090 MTMR3 myotubularin related M + P + T cytoplasm|hydrolase activity|inositol or 125b protein 3 phosphatidylinositol phosphatase activity|membrane|membrane fraction|phospholipid dephosphorylation|protein amino acid dephosphorylation|protein serine/threonine phosphatase activity|protein tyrosine phosphatase activity|protein tyrosine/serine/threonine phosphatase activity|zinc ion binding miR- AK024501 MXD4 MAX dimerization M + P + T DNA binding|negative regulation of cell 125b protein 4 proliferation|negative regulation of transcription from RNA polymerase II promoter|nucleus|protein binding|regulation of transcription, DNA- dependent|transcription|transcription corepressor activity miR- AB020642 MYT1 myelin transcription M + P + T nucleus|regulation of transcription, DNA- 125b factor 1 dependent|transcription|transcription factor activity|zinc ion binding miR- NM_004540 NCAM2 neural cell adhesion P + T cell adhesion|integral to membrane|membrane|neuron 125b molecule 2 adhesion|plasma membrane|protein binding miR- NM_012338 NET-2 transmembrane 4 P + T integral to membrane|membrane fraction 125b superfamily member tetraspan NET-2 miR- U84246 NEU1 sialidase 1 (lysosomal P + T carbohydrate metabolism|exo-alpha-sialidase 125b sialidase) activity|hydrolase activity, acting on glycosyl bonds|lysosome miR- AI824012 NRIP1 nuclear receptor P + T nucleus|regulation of transcription, DNA- 125b interacting protein 1 dependent|transcription|transcription coactivator activity miR- D81048 NRM nurim (nuclear envelope P + T 125b membrane protein) miR- BC001794 NUMBL numb homolog P + T neurogenesis 125b (Drosophila)-like miR- AB020713 NUP210 nucleoporin 210 P + T development|nucleus 125b miR- NM_002537 OAZ2 ornithine decarboxylase M + P + T ornithine decarboxylase inhibitor activity|polyamine 125b antizyme 2 metabolism miR- NM_024586 OSBPL9 oxysterol binding P + T lipid transport|steroid metabolism 125b protein-like 9 miR- U64661 PABP ESTs, Highly similar to P + T 125b PAB1_HUMAN Polyadenylate-binding protein 1 (Poly(A)- binding protein 1) (PABP 1) (PABP1) [H. sapiens] miR- AK000003 PCQAP PC2 (positive cofactor P + T 125b 2, multiprotein complex) glutamine/Q- rich-associated protein miR- NM_004716 PCSK7 proprotein convertase M + P + T integral to Golgi membrane|integral to 125b subtilisin/kexin type 7 membrane|peptidase activity|peptidase activity|peptide hormone processing|proteolysis and peptidolysis|subtilase activity miR- NM_006201 PCTK1 PCTAIRE protein M + P + T ATP binding|protein amino acid 125b kinase 1 phosphorylation|protein amino acid phosphorylation|protein serine/threonine kinase activity|protein serine/threonine kinase activity|regulation of cell cycle|transferase activity miR- NM_021213 PCTP phosphatidylcholine M + P + T cytosol|lipid binding|lipid 125b transfer protein transport|phosphatidylcholine transporter activity miR- NM_021255 PELI2 pellino homolog 2 M + P + T 125b (Drosophila) miR- NM_002646 PIK3C2B phosphoinositide-3- P + T inositol or phosphatidylinositol kinase 125b kinase, class 2, beta activity|intracellular signaling polypeptide cascade|microsome (phosphatidylinositol 3-kinase activity|phosphatidylinositol-4-phosphate 3-kinase activity|phosphoinositide 3-kinase complex|plasma membrane|transferase activity miR- NM_003628 PKP4 plakophilin 4 P + T cell adhesion|cytoskeleton|intercellular 125b junction|protein binding|structural molecule activity miR- NM_006718 PLAGL1 pleiomorphic adenoma P + T DNA binding|cell cycle arrest|induction of 125b gene-like 1 apoptosis|nucleic acid binding|nucleus|regulation of transcription, DNA-dependent|transcription|zinc ion binding miR- AI457120 PPAT phosphoribosyl P + T amidophosphoribosyltransferase activity|glutamine 125b pyrophosphate metabolism|magnesium ion amidotransferase binding|metabolism|nucleoside metabolism|purine base biosynthesis|purine nucleotide biosynthesis|transferase activity, transferring glycosyl groups miR- NM_002719 PPP2R5C protein phosphatase 2, P + T hydrolase activity|nucleus|phosphoprotein 125b regulatory subunit B phosphatase activity|protein phosphatase type 2A (B56), gamma isoform complex|protein phosphatase type 2A complex|protein phosphatase type 2A regulator activity|protein phosphatase type 2A regulator activity|signal transduction|signal transduction miR- AL022067 PRDM1 PR domain containing P + T 125b 1, with ZNF domain miR- U23736 PRDM2 PR domain containing P + T DNA binding|metal ion 125b 2, with ZNF domain binding|nucleus|nucleus|regulation of transcription|regulation of transcription, DNA- dependent|transcription factor activity|transcription regulator activity|zinc ion binding|zinc ion binding miR- AF083033 PRKRA protein kinase, P + T double-stranded RNA binding|enzyme activator 125b interferon-inducible activity|immune response|intracellular|kinase double stranded RNA activity|negative regulation of cell dependent activator proliferation|response to virus|signal transducer activity|signal transduction miR- NM_014369 PTPN18 protein tyrosine P + T hydrolase activity|non-membrane spanning protein 125b phosphatase, non- tyrosine phosphatase activity|protein amino acid receptor type 18 (brain- dephosphorylation|protein amino acid derived) dephosphorylation|protein tyrosine phosphatase activity miR- AI762627 PTPRF protein tyrosine P + T cell adhesion|hydrolase activity|integral to 125b phosphatase, receptor membrane|integral to plasma membrane|protein type, F amino acid dephosphorylation|protein binding|protein tyrosine phosphatase activity|receptor activity|transmembrane receptor protein tyrosine phosphatase activity|transmembrane receptor protein tyrosine phosphatase signaling pathway miR- NM_002840 PTPRF protein tyrosine P + T cell adhesion|hydrolase activity|integral to 125b phosphatase, receptor membrane|integral to plasma membrane|protein type, F amino acid dephosphorylation|protein binding|protein tyrosine phosphatase activity|receptor activity|transmembrane receptor protein tyrosine phosphatase activity|transmembrane receptor protein tyrosine phosphatase signaling pathway miR- AF142419 QKI homolog of mouse P + T 125b quaking QKI (KH domain RNA binding protein) miR- NM_004283 RAB3D RAB3D, member RAS P + T GTP binding|GTPase activity|exocytosis|hemocyte 125b oncogene family development|protein transport|small GTPase mediated signal transduction miR- BC002510 RAB6B RAB6B, member RAS P + T GTP binding|GTPase activity|Golgi 125b oncogene family apparatus|intracellular protein transport|retrograde transport, Golgi to ER|small GTPase mediated signal transduction miR- AK022662 RASAL2 RAS protein activator P + T GTPase activator activity|Ras GTPase activator 125b like 2 activity|signal transduction miR- NM_004841 RASAL2 RAS protein activator P + T GTPase activator activity|Ras GTPase activator 125b like 2 activity|signal transduction miR- NM_016090 RBM7 RNA binding motif P + T RNA binding|meiosis|nucleic acid binding|nucleotide 125b protein 7 binding miR- NM_006268 REQ requiem, apoptosis M + P + T DNA binding|apoptosis|induction of apoptosis by 125b response zinc finger extracellular signals|nucleus|protein gene ubiquitination|regulation of transcription, DNA- dependent|transcription|ubiquitin ligase complex|ubiquitin-protein ligase activity|zinc ion binding miR- NM_000449 RFX5 regulatory factor X, 5 P + T nucleus|regulation of transcription, DNA- 125b (influences HLA class dependent|transcription|transcription coactivator II expression) activity|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_003721 RFXANK regulatory factor X- P + T humoral immune response|nucleus|regulation of 125b associated ankyrin- transcription, DNA- containing protein dependent|transcription|transcription coactivator
activity|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_014746 RNF144 likely ortholog of P + T nucleus|protein ubiquitination|ubiquitin ligase 125b mouse ubiquitin complex|ubiquitin-protein ligase activity|zinc ion conjugating enzyme 7 binding interacting protein 4 miR- NM_014771 RNF40 ring finger protein 40 M + P + T protein ubiquitination|ubiquitin ligase 125b complex|ubiquitin-protein ligase activity|zinc ion binding miR- AL109955 RNPC1 RNA-binding region P + T 125b (RNP1, RRM) containing 1 miR- AF116627 RPL29 ribosomal protein L29 M + T 125b miR- NM_002953 RPS6KA1 ribosomal protein S6 M + P + T ATP binding|protein amino acid 125b kinase, 90 kDa, phosphorylation|protein serine/threonine kinase polypeptide 1 activity|protein serine/threonine kinase activity|protein-tyrosine kinase activity|signal transduction|transferase activity miR- NM_000332 SCA1 spinocerebellar ataxia 1 P + T RNA binding|cytoplasm|nucleus 125b (olivopontocerebellar ataxia 1, autosomal dominant, ataxin 1) miR- NM_012429 SEC14L2 SEC14-like 2 P + T cytoplasm|intracellular protein 125b (S. cerevisiae) transport|membrane|nucleus|phospholipid binding|positive regulation of transcription, DNA- dependent|protein carrier activity|regulation of cholesterol biosynthesis|transcription|transcriptional activator activity|transport|vitamin E binding miR- NM_005065 SEL1L sel-1 suppressor of lin- P + T catalytic activity|integral to membrane 125b 12-like (C. elegans) miR- NM_017789 SEMA4C sema domain, M + P + T cell differentiation|integral to 125b immunoglobulin membrane|membrane|neurogenesis|receptor activity domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C miR- NM_006378 SEMA4D sema domain, P + T anti-apoptosis|cell adhesion|cell 125b immunoglobulin differentiation|immune response|integral to domain (Ig), membrane|membrane|neurogenesis|receptor activity transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D miR- BE622841 SENP2 sentrin-specific protease M + P 125b miR- NM_003011 SET SET translocation M + T DNA replication|endoplasmic reticulum|histone 125b (myeloid leukemia- binding|negative regulation of histone associated) acetylation|nucleocytoplasmic transport|nucleosome assembly|nucleosome disassembly|nucleus|perinuclear region|protein phosphatase inhibitor activity|protein phosphatase type 2A regulator activity miR- NM_006275 SFRS6 splicing factor, P + T RNA binding|mRNA splice site selection|nuclear 125b arginine/serine-rich 6 mRNA splicing, via spliceosome|nucleotide binding|nucleus miR- AF015043 SH3BP4 SH3-domain binding P + T cell cycle|endocytosis|nucleus|signal transducer 125b protein 4 activity miR- NM_016538 SIRT7 sirtuin silent mating P + T DNA binding|chromatin silencing|chromatin silencing 125b type information complex|hydrolase activity|regulation of transcription, regulation 2 homolog 7 DNA-dependent (S. cerevisiae) miR- NM_020309 SLC17A7 solute carrier family 17 P + T integral to membrane|phosphate transport|sodium- 125b (sodium-dependent dependent phosphate transporter inorganic phosphate activity|transport|transporter activity cotransporter), member 7 miR- NM_013272 SLC21A11 solute carrier family 21 P + T integral to membrane|ion 125b (organic anion transport|membrane|transporter activity transporter), member 11 miR- AK000722 SLC27A4 solute carrier family 27 P + T catalytic activity|fatty acid transport|fatty acid 125b (fatty acid transporter), transporter activity|ligase activity|lipid member 4 metabolism|lipid transport|metabolism miR- NM_003759 SLC4A4 solute carrier family 4, P + T anion transport|inorganic anion exchanger 125b sodium bicarbonate activity|integral to membrane|integral to plasma cotransporter, member 4 membrane|membrane|sodium:bicarbonate symporter activity|transport miR- NM_003045 SLC7A1 solute carrier family 7 P + T amino acid metabolism|amino acid permease 125b (cationic amino acid activity|amino acid transport|basic amino acid transporter, y+ system), transporter activity|integral to plasma member 1 membrane|membrane|receptor activity|transport miR- NM_003983 SLC7A6 solute carrier family 7 P + T amino acid metabolism|amino acid transport|amino 125b (cationic amino acid acid-polyamine transporter activity|integral to plasma transporter, y+ system), membrane|plasma membrane|protein complex member 6 assembly|transport miR- AF113019 SMARCD2 SWI/SNF related, M + P + T chromatin remodeling|nucleoplasm|regulation of 125b matrix associated, actin transcription from RNA polymerase II dependent regulator of promoter|transcription|transcription coactivator chromatin, subfamily d, activity member 2 miR- NM_005985 SNAI1 snail homolog 1 P + T DNA binding|cartilage 125b (Drosophila) condensation|development|neurogenesis|nucleus|zinc ion binding miR- AB037750 SORCS2 VPS10 domain receptor P + T integral to membrane|intracellular protein 125b protein transport|membrane|membrane|neuropeptide receptor activity|neuropeptide signaling pathway|protein binding|protein transporter activity|sugar binding miR- BE742268 SORT1 sortilin 1 P + T endocytosis|endosome|integral to membrane|integral 125b to membrane|intracellular protein transport|membrane|neurotensin receptor activity, G- protein coupled|protein transporter activity|receptor activity miR- AI360875 SOX11 SRY (sex determining M + T DNA binding|neurogenesis|nucleus|regulation of 125b region Y)-box 11 transcription, DNA-dependent|transcription miR- AU121035 SP1 Sp1 transcription factor P + T DNA binding|RNA polymerase II transcription factor 125b activity|nucleus|regulation of transcription, DNA- dependent|transcription|transcriptional activator activity|zinc ion binding miR- NM_003131 SRF serum response factor M + T RNA polymerase II transcription factor 125b (c-fos serum response activity|nucleus|regulation of transcription from RNA element-binding polymerase II promoter|signal transcription factor) transduction|transcription|transcription factor activity miR- NM_005637 SS18 synovial sarcoma P + T nucleus 125b translocation, chromosome 18 miR- AF343880 SSX2 synovial sarcoma, X P + T nucleus 125b breakpoint 2 miR- NM_014682 ST18 suppression of P + T nucleus|regulation of transcription, DNA- 125b tumorigenicity 18 dependent|transcription factor activity (breast carcinoma) (zinc finger protein) miR- AA128023 STARD13 START domain P + T 125b containing 13 miR- BC000627 STAT3 signal transducer and P + T JAK-STAT cascade|acute-phase response|calcium ion 125b activator of binding|cell transcription 3 (acute- motility|cytoplasm|hematopoietin/interferon-class phase response factor) (D200-domain) cytokine receptor signal transducer activity|intracellular signaling cascade|negative regulation of transcription from RNA polymerase II promoter|neurogenesis|nucleus|nucleus|regulation of transcription, DNA-dependent|signal transducer activity|transcription|transcription factor activity|transcription factor activity miR- NM_003155 STC1 stanniocalcin 1 P + T calcium ion homeostasis|cell surface receptor linked 125b signal transduction|cell-cell signaling|extracellular region|hormone activity|response to nutrients miR- NM_003173 SUV39H1 suppressor of P + T DNA replication and chromosome cycle|S- 125b variegation 3-9 adenosylmethionine-dependent methyltransferase homolog 1 (Drosophila) activity|chromatin|chromatin assembly or disassembly|chromatin binding|chromatin modification|condensed nuclear chromosome|histone lysine N-methyltransferase activity (H3-K9 specific)|histone-lysine N-methyltransferase activity|methyltransferase activity|nucleus|nucleus|protein binding|transferase activity|zinc ion binding miR- AW139618 SYN2 synapsin II P + T neurotransmitter secretion|synapse|synaptic 125b transmission|synaptic vesicle miR- R60550 TAF5L TAF5-like RNA M + P + T nucleus|regulation of transcription, DNA- 125b polymerase II, dependent|transcription factor activity|transcription p300/CBP-associated from RNA polymerase II promoter factor (PCAF)- associated factor, 65 kDa miR- AF220509 TAF9L TAF9-like RNA P + T DNA binding|nucleus|regulation of transcription, 125b polymerase II, TATA DNA-dependent|transcription factor TFIID box binding protein complex|transcription initiation (TBP)-associated factor, 31 kDa miR- NM_000116 TAZ tafazzin M + P + T acyltransferase activity|heart development|integral to 125b (cardiomyopathy, membrane|metabolism|muscle contraction|muscle dilated 3A (X-linked); development endocardial fibroelastosis 2; Barth syndrome) miR- NM_018488 TBX4 T-box 4 P + T development|nucleus|regulation of transcription, 125b DNA-dependent|transcription|transcription factor activity miR- NM_012249 TC10 ras-like protein TC10 M + T GTP binding|GTPase activity|plasma membrane|small 125b GTPase mediated signal transduction miR- BG387172 TEAD2 TEA domain family P + T nucleus|nucleus|regulation of transcription, DNA- 125b member 2 dependent|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcription factor activity miR- U06935 TEF thyrotrophic embryonic P + T RNA polymerase II transcription factor 125b factor activity|nucleus|regulation of transcription from RNA polymerase II promoter|rhythmic process|transcription|transcription factor activity miR- NM_006464 TGOLN2 trans-golgi network P + T Golgi trans face|integral to membrane|transport 125b protein 2 vesicle miR- BE219311 TIMM22 translocase of inner P + T integral to membrane|mitochondrial inner 125b mitochondrial membrane|mitochondrion|protein transport|protein membrane 22 homolog transporter activity (yeast) miR- NM_003326 TNFSF4 tumor necrosis factor P + T cell-cell signaling|immune response|integral to plasma 125b (ligand) superfamily, membrane|membrane|positive regulation of cell member 4 (tax- proliferation|signal transduction|tumor necrosis factor transcriptionally receptor binding activated glycoprotein 1, 34 kDa) miR- AA873275 TOR2A torsin family 2, member A P + T ATP binding|GTP cyclohydrolase I 125b activity|biosynthesis|chaperone cofactor dependent protein folding|endoplasmic reticulum|nucleoside- triphosphatase activity|nucleotide binding miR- AW341649 TP53INP1 tumor protein p53 M + P + T apoptosis|nucleus 125b inducible nuclear protein 1 miR- NM_014112 TRPS1 trichorhinophalangeal P + T NLS-bearing
substrate-nucleus 125b syndrome I import|nucleus|regulation of transcription, DNA- dependent|skeletal development|transcription|transcription factor activity|transcription from RNA polymerase II promoter|zinc ion binding miR- NM_001070 TUBG1 tubulin, gamma 1 P + T GTP binding|GTPase activity|centrosome|condensed 125b nuclear chromosome|gamma-tubulin complex|meiotic spindle organization and biogenesis|microtubule|microtubule nucleation|microtubule-based movement|mitotic spindle organization and biogenesis|polar microtubule|protein binding|protein polymerization|spindle pole body|structural constituent of cytoskeleton miR- NM_003330 TXNRD1 thioredoxin reductase 1 P + T FAD binding|cell redox 125b homeostasis|cytoplasm|disulfide oxidoreductase activity|electron transport|electron transporter activity|oxidoreductase activity, acting on NADH or NADPH, disulfide as acceptor|signal transduction|thioredoxin-disulfide reductase activity miR- BC004862 UBE2R2 ubiquitin-conjugating P + T ligase activity|ubiquitin conjugating enzyme 125b enzyme E2R 2 activity|ubiquitin cycle|ubiquitin-protein ligase activity miR- NM_003728 UNC5C unc-5 homolog B P + T apoptosis|axon guidance|brain 125b (C. elegans) development|development|integral to membrane |netrin receptor activity|protein binding|receptor activity|signal transduction miR- NM_003369 UVRAG UV radiation resistance P + T DNA repair|cytoplasm 125b associated gene miR- AF195514 VPS4B vacuolar protein sorting M + P + T ATP binding|ATPase activity, 125b 4B (yeast) coupled|membrane|membrane fusion|nucleoside- triphosphatase activity|nucleotide binding|peroxisome organization and biogenesis|protein binding|regulation of transcription, DNA-dependent miR- R51061 VTS58635 mitogen-activated P + T GTP binding|small GTPase mediated signal 125b protein kinase kinase transduction kinase kinase 1 miR- NM_004184 WARS tryptophanyl-tRNA M + T ATP binding|cytoplasm|ligase activity|negative 125b synthetase regulation of cell proliferation|protein biosynthesis|soluble fraction|tryptophan-tRNA ligase activity|tryptophanyl-tRNA aminoacylation|tryptophanyl-tRNA aminoacylation miR- NM_005433 YES1 v-yes-1 Yamaguchi P + T ATP binding|intracellular signaling cascade|protein 125b sarcoma viral oncogene amino acid phosphorylation|protein-tyrosine kinase homolog 1 activity|transferase activity miR- NM_017740 ZDHHC7 zinc finger, DHHC P + T integral to membrane|metal ion binding 125b domain containing 7 miR- BF525395 ZFP385 likely ortholog of M + P + T DNA binding|nucleic acid binding|nucleus|regulation 125b mouse zinc finger of transcription, DNA-dependent|transcription|zinc protein 385 ion binding miR- NM_007345 ZNF236 zinc finger protein 236 P + T nucleus|regulation of transcription, DNA- 125b dependent|transcription|transcription factor activity|zinc ion binding miR- NM_012482 ZNF281 zinc finger protein 281 M + P + T DNA binding|DNA-directed RNA polymerase II, core 125b complex|negative regulation of transcription from RNA polymerase II promoter|nucleus|regulation of transcription, DNA-dependent|specific RNA polymerase II transcription factor activity|transcription|zinc ion binding miR- NM_003427 ZNF76 zinc finger protein 76 P + T DNA binding|nucleus|regulation of transcription from 125b (expressed in testis) RNA polymerase II promoter|regulation of transcription from RNA polymerase III promoter|transcription|zinc ion binding miR- NM_022465 ZNFN1A4 zinc finger protein, M + P + T nucleic acid binding|nucleus|transcription factor 125b subfamily 1A, 4 (Eos) activity|transcriptional repressor activity|zinc ion binding miR- NM_005502 ABCA1 ATP-binding cassette, P + T ATP binding|ATP binding|ATPase activity|anion 145 sub-family A (ABC1), transporter activity|cholesterol metabolism|integral to member 1 plasma membrane|lipid metabolism|membrane fraction|nucleotide binding|steroid metabolism|sterol transporter activity|transport|transport miR- AL527773 ABR active BCR-related M + P + T GTPase activator activity|guanyl-nucleotide exchange 145 gene factor activity|small GTPase mediated signal transduction miR- NM_001616 ACVR2 activin A receptor, type M + P + T ATP binding|integral to plasma 145 II membrane|membrane|protein amino acid phosphorylation|receptor activity|transferase activity|transforming growth factor beta receptor activity|transmembrane receptor protein serine/threonine kinase signaling pathway miR- NM_003183 ADAM17 a disintegrin and P + T cell-cell signaling|integral to plasma 145 metalloproteinase membrane|metalloendopeptidase activity|proteolysis domain 17 (tumor and peptidolysis|zinc ion binding necrosis factor, alpha, converting enzyme) miR- NM_019903 ADD3 adducin 3 (gamma) M + P + T calmodulin binding|cytoskeleton|membrane|structural 145 constituent of cytoskeleton miR- AB003476 AKAP12 A kinase (PRKA) P + T G-protein coupled receptor protein signaling 145 anchor protein (gravin) pathway|cytoplasm|protein binding|protein kinase A 12 binding|protein targeting|signal transduction miR- NM_016201 AMOTL2 angiomotin like 2 M + P + T 145 miR- NM_001128 AP1G1 adaptor-related protein M + P + T Golgi apparatus|binding|clathrin coat of trans-Golgi 145 complex 1, gamma 1 network vesicle|coated pit|endocytosis|intracellular subunit protein transport|intracellular protein transport|membrane coat adaptor complex|protein complex assembly|transporter activity miR- NM_001284 AP3S1 adaptor-related protein M + P + T Golgi apparatus|clathrin vesicle coat|insulin receptor 145 complex 3, sigma 1 signaling pathway|intracellular protein subunit transport|membrane coat adaptor complex|transport|transport vesicle|transporter activity miR- NM_006380 APPBP2 amyloid beta precursor M + P + T binding|cytoplasm|intracellular protein 145 protein (cytoplasmic transport|membrane|microtubule associated tail) binding protein 2 complex|microtubule motor activity|nucleus miR- AB037845 ARHGAP10 Rho-GTPase activating M + T protein binding 145 protein 10 miR- AL516350 ARPC5 actin related protein 2/3 P + T Arp2/3 protein complex|actin cytoskeleton 145 complex, subunit 5, organization and biogenesis|cell 16 kDa motility|cytoplasm|cytoskeleton|regulation of actin filament polymerization|structural constituent of cytoskeleton miR- U72937 ATRX alpha M + T ATP binding|DNA binding|DNA helicase 145 thalassemia/mental activity|DNA methylation|DNA recombination|DNA retardation syndrome repair|chromosome organization and biogenesis X-linked (RAD54 (sensu Eukaryota)|helicase activity|hydrolase homolog, S. cerevisiae) activity|nuclear heterochromatin|nucleus|perception of sound|regulation of transcription, DNA- dependent|transcription factor activity miR- NM_021813 BACH2 BTB and CNC P + T DNA binding|nucleus|protein binding|regulation of 145 homology 1, basic transcription, DNA-dependent|transcription leucine zipper transcription factor 2 miR- NM_013449 BAZ2A bromodomain adjacent P + T DNA binding|chromatin remodeling|nucleolus 145 to zinc finger domain, organizer complex|nucleus|regulation of transcription, 2A DNA-dependent|transcription|transcription regulator activity miR- NM_007005 BCE-1 BCE-1 protein M + P frizzled signaling pathway|molecular_function 145 unknown|nucleus|nucleus|regulation of transcription|regulation of transcription, DNA- dependent miR- NM_003458 BSN bassoon (presynaptic P + T cytoskeleton|metal ion binding|nucleus|structural 145 cytomatrix protein) constituent of cytoskeleton|synapse|synaptic transmission|synaptosome miR- NM_013279 C11orf9 chromosome 11 open M + P + T 145 reading frame 9 miR- NM_024643 C14orf140 hypothetical protein P + T 145 FLJ23093 miR- NM_018270 C20orf20 chromosome 20 open P + T chromatin modification|nucleus|regulation of cell 145 reading frame 20 growth|regulation of transcription, DNA- dependent|transcription miR- NM_004276 CABP1 calcium binding protein P + T calcium ion binding|calcium ion binding|enzyme 145 1 (calbrain) inhibitor activity miR- NM_001755 CBFB core-binding factor, M + P + T RNA polymerase II transcription factor 145 beta subunit activity|nucleus|transcription coactivator activity|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_001759 CCND2 cyclin D2 P + T cytokinesis|nucleus|regulation of cell cycle 145 miR- NM_020307 CCNL1 cyclin L ania-6a M + P + T cell cycle|regulation of cell cycle 145 miR- AL118798 CD47 CD47 antigen (Rh- P + T cell-matrix adhesion|integral to plasma 145 related antigen, membrane|integrin-mediated signaling integrin-associated pathway|plasma membrane|protein binding signal transducer) miR- BF576053 CFL2 cofilin 2 (muscle) M + P + T actin binding|cytoskeleton|nucleus 145 miR- AA835485 CKLiK CamKI-like protein P + T ATP binding|calcium- and calmodulin-dependent 145 kinase protein kinase activity|calmodulin binding|nucleus|protein amino acid phosphorylation|protein serine/threonine kinase activity|transferase activity miR- NM_004921 CLCA3 chloride channel, P + T extracellular space|transport|transporter activity 145 calcium activated, family member 3 miR- NM_001326 CSTF3 cleavage stimulation M + P + T RNA binding|binding|mRNA cleavage|mRNA 145 factor, 3' pre-RNA, polyadenylylation|nucleus subunit 3, 77 kDa miR- NM_020248 CTNNBIP1 catenin, beta interacting P + T Wnt receptor signaling pathway|beta-catenin 145 protein 1 binding|cell proliferation|development|nucleus|regulation of transcription, DNA-dependent|signal transduction miR- AW772082 DACH dachshund homolog P + T DNA binding|development|eye morphogenesis (sensu 145 (Drosophila) Endopterygota)|nucleus|regulation of transcription, DNA-dependent|transcription miR- NM_004393 DAG1 dystroglycan 1 M + P + T actin cytoskeleton|calcium ion binding|extracellular 145 (dystrophin-associated matrix (sensu Metazoa)|integral to plasma glycoprotein 1) membrane|laminin receptor activity|membrane fraction|muscle contraction|plasma membrane|protein binding|protein complex assembly miR- NM_003887 DDEF2 development and P + T GTPase activator activity|Golgi apparatus|regulation 145 differentiation of GTPase activity enhancing factor 2 miR- AL080239 DKFZp547M2010 hypothetical protein M + P + T 145 DKFZp547M2010 miR- AL137517 DKFZp564O1278 hypothetical protein P + T integral to membrane 145 DKFZp564O1278 miR- NM_001386 DPYSL2 dihydropyrimidinase- P + T dihydropyrimidinase activity|hydrolase 145 like 2 activity|neurogenesis|nucleobase, nucleoside, nucleotide and nucleic acid metabolism|signal transduction miR- BC003143 DUSP6 dual specificity P + T MAP kinase phosphatase activity|cytoplasm|hydrolase 145 phosphatase 6 activity|inactivation of MAPK|protein amino acid dephosphorylation|protein serine/threonine phosphatase activity|protein tyrosine phosphatase activity|regulation of cell cycle|soluble fraction miR- D86550 DYRK1A dual-specificity P + T ATP binding|neurogenesis|nucleus|protein amino acid
145 tyrosine-(Y)- phosphorylation|protein serine/threonine kinase phosphorylation activity|protein-tyrosine kinase activity|transferase regulated kinase 1A activity miR- NM_001967 EIF4A2 eukaryotic translation M + P + T ATP binding|ATP-dependent helicase activity|DNA 145 initiation factor 4A, binding|RNA binding|eukaryotic translation initiation isoform 2 factor 4F complex|hydrolase activity|protein biosynthesis|regulation of translational initiation|translation initiation factor activity miR- NM_001417 EIF4B eukaryotic translation M + T RNA binding|eukaryotic translation initiation factor 145 initiation factor 4B 4F complex|nucleic acid binding|nucleotide binding|protein biosynthesis|regulation of translational initiation|translation initiation factor activity|translation initiation factor activity miR- BC005057 EIF4EBP2 eukaryotic translation P + T eukaryotic initiation factor 4E binding|negative 145 initiation factor 4E regulation of protein biosynthesis|negative regulation binding protein 2 of translational initiation|regulation of translation miR- NM_020909 EPB41L5 erythrocyte membrane P + T binding|cytoplasm|cytoskeletal protein 145 protein band 4.1 like 5 binding|cytoskeleton|membrane miR- NM_005797 EVA1 epithelial V-like antigen 1 P + T cell adhesion|cytoskeleton|homophilic cell 145 adhesion|integral to membrane|membrane|morphogenesis|protein binding miR- NM_022977 FACL4 fatty-acid-Coenzyme A M + P + T fatty acid metabolism|integral to membrane|learning 145 ligase, long-chain 4 and/or memory|ligase activity|lipid metabolism|long- chain-fatty-acid-CoA ligase activity|magnesium ion binding|metabolism miR- AL042120 FHOD2 formin homology 2 M + P Rho GTPase binding|actin binding|actin cytoskeleton 145 domain containing 2 organization and biogenesis|cell organization and biogenesis|nucleus|regulation of transcription, DNA- dependent|transcription factor activity|translation initiation factor activity|translational initiation miR- NM_002013 FKBP3 FK506 binding protein P + T FK506 binding|isomerase activity|nucleus|peptidyl- 145 3, 25 kDa prolyl cis-trans isomerase activity|protein folding|receptor activity miR- NM_002017 FLI1 Friend leukemia virus M + P + T hemostasis|nucleus|organogenesis|regulation of 145 integration 1 transcription, DNA- dependent|transcription|transcription factor activity miR- NM_023071 FLJ13117 hypothetical protein P + T 145 FLJ13117 miR- AL561281 FLJ20373 hypothetical protein M + P + T ATP binding|cellular_component unknown|protein 145 FLJ20373 amino acid phosphorylation|protein kinase cascade|protein serine/threonine kinase activity|response to stress|signal transduction|small GTPase regulator activity|transferase activity miR- AK025444 FLJ21791 hypothetical protein M + T 145 FLJ21791 miR- NM_024713 FLJ22557 hypothetical protein P + T 145 FLJ22557 miR- AA872588 FLJ36155 likely ortholog of P + T DNA binding|negative regulation of transcription 145 mouse Gli-similar 1 from RNA polymerase II promoter|nucleus|positive Kruppel-like zinc finger regulation of transcription from RNA polymerase II (Glis1) promoter|regulation of transcription, DNA- dependent|specific RNA polymerase II transcription factor activity|transcription|zinc ion binding miR- AI434509 FLJ38499 Unnamed protein P + T nucleic acid binding 145 product [Homo sapiens], mRNA sequence miR- M62994 FLNB filamin B, beta (actin P + T actin binding|actin binding|actin cytoskeleton|actin 145 binding protein 278) cytoskeleton organization and biogenesis|cell differentiation|cytoskeletal anchoring|integral to plasma membrane|myogenesis|signal transduction miR- NM_002025 FMR2 fragile X mental M + T brain development|learning and/or memory 145 retardation 2 miR- N29672 FOS v-fos FBJ murine M + T proto-oncogene 145 osteosarcoma viral oncogene homolog miR- NM_002015 FOX01A forkhead box O1A M + P + T anti-apoptosis|nucleus|regulation of transcription from 145 (rhabdomyosarcoma) RNA polymerase II promoter|transcription|transcription factor activity miR- NM_003507 FZD7 frizzled homolog 7 M + P + T G-protein coupled receptor activity|G-protein coupled 145 (Drosophila) receptor protein signaling pathway|Wnt receptor activity|development|frizzled signaling pathway|integral to membrane|plasma membrane miR- AL049709 GGTL3 gamma- M + P + T 145 glutamyltransferase-like 3 miR- NM_022735 GOCAP1 golgi complex M + P + T Golgi apparatuslacyl-CoA binding|catalytic 145 associated protein 1, activity|intracellular protein 60 kDa transport|membrane|mitochondrion|protein carrier activity|steroid biosynthesis miR- NM_020806 GPHN gephyrin P + T Mo-molybdopterin cofactor biosynthesis|catalytic 145 activity|cytoskeleton miR- NM_015071 GRAF GTPase regulator P + T Rho GTPase activator activitylactin cytoskeleton 145 associated with focal organization and biogenesis|cellular_component adhesion kinase unknown|neurogenesis pp125(FAK) miR- NM_017913 HARC Hsp90-associating P + T cytokinesis|regulation of cell cycle 145 relative of Cdc37 miR- BC006237 HECTD1 HECT domain M + T intracellular|ligase activity|receptor activity|ubiquitin 145 containing 1 cycle|ubiquitin-protein ligase activity miR- U64317 HEF1 enhancer of P + T actin filament bundle formation|cell 145 filamentation 1 (cas-like adhesion|cytokinesis|cytoplasm|cytoskeleton| docking; Crk-associated cytoskeleton organization and biogenesis|integrin- substrate related) mediated signaling pathway|mitosis|nucleus| protein binding|regulation of cell cycle|regulation of cell growth|signal transduction|spindle miR- NM_016258 HGRG8 high-glucose-regulated P + T 145 protein 8 miR- AL162003 HIC2 hypermethylated in P + T DNA binding|negative regulation of transcription, 145 cancer 2 DNA-dependent|nucleus|protein C-terminus binding|transcription|zinc ion binding miR- NM_014212 HOXC11 homeo box C11 M + P + T RNA polymerase II transcription factor 145 activity|development|endoderm development|nucleus|regulation of transcription, DNA-dependent|transcription factor activity miR- NM_002193 INHBB inhibin, beta B (activin M + P + T cell differentiation|cytokine activity|defense 145 AB beta polypeptide) response|extracellular region|growth|growth factor activity|hormone activity|host cell surface receptor binding|negative regulation of follicle-stimulating hormone secretion|negative regulation of hepatocyte growth factor biosynthesis|ovarian follicle development|positive regulation of follicle- stimulating hormone secretion|protein binding|protein homodimerization activity|response to external stimulus miR- NM_005544 IRS1 insulin receptor M + P + T cytoplasm|insulin receptor binding|protein 145 substrate 1 binding|signal transducer activity|signal transduction|transmembrane receptor protein tyrosine kinase docking protein activity miR- NM_006459 KEO4 similar to P + T catalytic activity 145 Caenorhabditis elegans protein C42C1.9 miR- NM_014686 KIAA0355 KIAA0355 gene P + T 145 product miR- NM_015176 KIAA0483 KIAA0483 protein P + T ubiquitin cycle 145 miR- NM_014871 KIAA0710 KIAA0710 gene M + P + T cysteine-type endopeptidase activity|exonuclease 145 product activity|nucleus|ubiquitin cycle|ubiquitin thiolesterase activity|ubiquitin-dependent protein catabolism miR- AA772278 KIAA1673 KIAA1673 M + P + T 145 miR- AB051495 KIAA1708 KIAA1708 protein P + T ATP binding|microtubule associated 145 complex|microtubule motor activity|microtubule- based movement miR- AI814587 KIAA1715 KIAA1715 protein M + T 145 miR- AI187364 KIAA1894 KIAA1894 protein P + T integral to membrane 145 miR- AF155117 KIF21A kinesin family member P + T ATP binding|microtubule associated 145 21A complex|microtubule motor activity|microtubule- based movement miR- NM_004235 KLF4 Kruppel-like factor 4 M + T mesodermal cell fate determination|negative 145 (gut) regulation of cell proliferation|negative regulation of transcription, DNA-dependent|negative regulation of transcription, DNA-dependent|nucleic acid binding|nucleus|transcription|transcription factor activity|transcription factor activity|transcriptional activator activity|transcriptional activator activity|transcriptional repressor activity|transcriptional repressor activity|zinc ion binding|zinc ion binding miR- T68150 LL5beta hypothetical protein M + T 145 FLJ21791 miR- AI797833 LOC285148 a disintegrin and P + T catalytic activity 145 metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) miR- NM_025146 MAK3P likely ortholog of P + T N-acetyltransferase activity 145 mouse Mak3p homolog (S. cerevisiae) miR- BF971923 MAP3K3 mitogen-activated M + P ATP binding|MAP kinase kinase kinase 145 protein kinase kinase activity|MAPKKK cascade|magnesium ion kinase 3 binding|positive regulation of I-kappaB kinase/NF- kappaB cascade|protein amino acid phosphorylation|protein kinase activity|protein serine/threonine kinase activity|signal transducer activity|transferase activity miR- NM_004834 MAP4K4 mitogen-activated M + P + T ATP binding|cellular_component unknown|protein 145 protein kinase kinase amino acid phosphorylation|protein kinase kinase kinase 4 cascade|protein serine/threonine kinase activity|response to stress|signal transduction|small GTPase regulator activity|transferase activity miR- BF382281 MGC10120 Homo sapiens cDNA P + T 145 FLJ30135 fis, clone BRACE2000061, mRNA sequence miR- BG231756 MGC10986 hypothetical protein M + P ATP binding|MAP kinase kinase kinase 145 MGC10986 activity|MAPKKK cascade|magnesium ion binding|positive regulation of I-kappaB kinase/NF- kappaB cascade|protein amino acid phosphorylation|protein kinase activity|protein serine/threonine kinase activity|signal transducer activity|transferase activity miR- BC004869 MGC2817 hypothetical protein P + T outer membrane|protein transport 145 MGC2817 miR- BC002712 MYCN v-myc M + T chromatin|nucleus|protein binding|regulation of 145 myelocytomatosis viral transcription from RNA polymerase II related oncogene, promoter|transcription factor activity neuroblastoma derived (avian) miR- AB007899 NEDD4L neural precursor cell P + T excretion|intracellular|intracellular|ligase 145 expressed, activity|positive regulation of endocytosis|protein developmentally down- binding|protein ubiquitination|regulation of protein regulated 4-like catabolism|response to metal ion|sodium channel regulator activity|sodium ion homeostasis|sodium ion transport|ubiquitin cycle|ubiquitin-protein ligase activity|ubiquitin-protein ligase activity|water homeostasis miR- NM_005863 NET1 neuroepithelial cell P + T guanyl-nucleotide exchange factor 145 transforming gene 1 activity|nucleus|regulation of cell growth|signal transduction miR- NM_003204 NFE2L1 nuclear factor P + T DNA binding|heme
biosynthesis|inflammatory 145 (erythroid-derived 2)- response|morphogenesis|nucleus|nucleus|regulation of like 1 transcription, DNA- dependent|transcription|transcription cofactor activity|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_006469 NS1-BP NS1-binding protein M + P + T RNA splicing|protein binding|response to 145 virus|spliceosome complex|transcription factor complex|transcription from RNA polymerase III promoter miR- NM_019094 NUDT4 nudix (nucleoside P + T calcium-mediated signaling|cyclic nucleotide 145 diphosphate linked metabolism|cyclic-nucleotide-mediated moiety X)-type motif 4 signaling|diphosphoinositol-polyphosphate diphosphatase activity|hydrolase activity|intracellular|intracellular signaling cascade|intracellular transport|magnesium ion binding|regulation of RNA-nucleus export miR- AW149417 OAZ OLF-1/EBF associated P + T nucleic acid binding|nucleus|zinc ion binding 145 zinc finger gene miR- NM_024586 OSBPL9 oxysterol binding M + P lipid transport|steroid metabolism 145 protein-like 9 miR- AB040812 PAK7 p21(CDKN1A)- M + T ATP binding|protein amino acid 145 activated kinase 7 phosphorylation|protein serine/threonine kinase activity|transferase activity miR- NM_014456 PDCD4 programmed cell death M + P + T apoptosis 145 4 (neoplastic transformation inhibitor) miR- NM_002657 PLAGL2 pleiomorphic adenoma M + P + T nucleus|regulation of transcription, DNA- 145 gene-like 2 dependent|transcription|transcription factor activity|zinc ion binding miR- AK023546 PLCL2 phospholipase C-like 2 P + T calcium ion binding|intracellular signaling 145 cascade|lipid metabolism|phosphoinositide phospholipase C activity miR- AI274352 PLN phospholamban P + T 145 miR- NM_000944 PPP3CA protein phosphatase 3 P + T calcineurin complex|calcium ion binding|calmodulin 145 (formerly 2B), catalytic binding|hydrolase activity|protein amino acid subunit, alpha isoform dephosphorylation|protein serine/threonine (calcineurin A alpha) phosphatase activity miR- BF247371 PRO1843 hypothetical protein M + T 145 PRO1843 miR- NM_000959 PTGFR prostaglandin F receptor P + T G-protein coupled receptor protein signaling 145 (FP) pathway|G-protein coupled receptor protein signaling pathway|integral to membrane|integral to plasma membrane|parturition|prostaglandin F receptor activity|prostaglandin F receptor activity|receptor activity|rhodopsin-like receptor activity|signal transduction|thromboxane receptor activity miR- NM_002890 RASA1 RAS p21 protein P + T Ras GTPase activator activity|intracellular signaling 145 activator (GTPase cascade activating protein) 1 miR- NM_006506 RASA2 RAS p21 protein P + T Ras GTPase activator activity|intracellular signaling 145 activator 2 cascade miR- NM_002912 REV3L REV3-like, catalytic M + P + T 3'-5' exonuclease activity|DNA binding|DNA 145 subunit of DNA repair|DNA replication|DNA-dependent DNA polymerase zeta (yeast) replication|DNA-directed DNA polymerase activity|nucleotide binding|nucleus|transferase activity|zeta DNA polymerase activity|zeta DNA polymerase complex miR- NM_002924 RGS7 regulator of G-protein P + T heterotrimeric G-protein complex|intracellular 145 signalling 7 signaling cascade|regulation of G-protein coupled receptor protein signaling pathway|regulator of G- protein signaling activity|signal transducer activity miR- AL136924 RIN2 Ras and Rab interactor 2 P + T GTPase activator activity|Rab guanyl-nucleotide 145 exchange factor activity|cellular_component unknown|endocytosis|intracellular signaling cascade|small GTPase mediated signal transduction|small GTPase regulator activity miR- BE463945 RTKN rhotekin P + T intracellular|protein binding|signal transduction|signal 145 transduction miR- AF225986 SCN3A sodium channel, P + T cation channel activity|cation transport|integral to 145 voltage-gated, type III, membrane|membrane|sodium ion transport|voltage- alpha polypeptide gated sodium channel activity|voltage-gated sodium channel complex miR- NM_006080 SEMA3A sema domain, P + T cell differentiation|extracellular region|neurogenesis 145 immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A miR- NM_020796 SEMA6A sema domain, P + T apoptosis|axonlaxon guidance|cell differentiation|cell 145 transmembrane domain surface receptor linked signal (TM), and cytoplasmic transduction|cytoskeleton organization and domain, (semaphorin) biogenesis|development|integral to 6A membrane|membrane|neurogenesis|protein binding|receptor activity miR- NM_004171 SLC1A2 solute carrier family 1 P + T L-glutamate transport|L-glutamate transporter 145 (glial high affinity activity|dicarboxylic acid transport|integral to glutamate transporter), membrane|membrane|membrane member 2 fraction|sodium:dicarboxylate symporter activity|symporter activity|synaptic transmission|transport miR- NM_003759 SLC4A4 solute carrier family 4, P + T anion transport|inorganic anion exchanger 145 sodium bicarbonate activity|integral to membrane|integral to plasma cotransporter, member 4 membrane|membrane|sodium:bicarbonate symporter activity|transport miR- NM_030918 SNX27 hypothetical protein M + P + T intracellular signaling cascade|protein binding|protein 145 My014 transport miR- AI360875 SOX11 SRY (sex determining M + T DNA binding|neurogenesis|nucleus|regulation of 145 region Y)-box 11 transcription, DNA-dependent|transcription miR- NM_000346 SOX9 SRY (sex determining P + T DNA binding|cartilage 145 region Y)-box 9 condensation|nucleus|regulation of transcription from (campomelic dysplasia, RNA polymerase II promoter|skeletal autosomal sex-reversal) development|specific RNA polymerase II transcription factor activity|transcription miR- AK023899 SRGAP1 SLIT-ROBO Rho P + T GTPase activator activity 145 GTPase activating protein 1 miR- NM_003155 STC1 stanniocalcin 1 M + T calcium ion homeostasis|cell surface receptor linked 145 signal transduction|cell-cell signaling|extracellular region|hormone activity|response to nutrients miR- BE219311 TIMM22 translocase of inner M + P + T integral to membrane|mitochondrial inner 145 mitochondrial membrane|mitochondrion|protein transport|protein membrane 22 homolog transporter activity (yeast) miR- AA705845 TLE4 transducin-like M + P frizzled signaling pathway|molecular_function 145 enhancer of split 4 unknown|nucleus|nucleus|regulation of (E(sp1) homolog, transcription|regulation of transcription, DNA- Drosophila) dependent miR- BC005016 TRIM2 tripartite motif- P + T cytoplasm|myosin binding|protein 145 containing 2 ubiquitination|ubiquitin ligase complex|ubiquitin- protein ligase activity|zinc ion binding miR- NM_025076 UXS1 UDP-glucuronate M + P + T carbohydrate metabolism|isomerase 145 decarboxylase 1 activity|nucleotide-sugar metabolism miR- NM_005433 YES1 v-yes-1 Yamaguchi P + T ATP binding|intracellular signaling cascade|protein 145 sarcoma viral oncogene amino acid phosphorylation|protein-tyrosine kinase homolog 1 activity|transferase activity miR- BC003128 ZDHHC9 zinc finger, DHHC P + T integral to membrane|metal ion binding 145 domain containing 9 miR- NM_019903 ADD3 adducin 3 (gamma) P + T calmodulin binding|cytoskeleton|membrane|structural 155 constituent of cytoskeleton miR- NM_020661 AICDA activation-induced P + T B-cell differentiation|cellular_component 155 cytidine deaminase unknown|cytidine deaminase activity|hydrolase activity|mRNA processing|zinc ion binding miR- NM_007202 AKAP10 A kinase (PRKA) P + T kinase activity|mitochondrion|protein binding|protein 155 anchor protein 10 localization|signal transducer activity|signal transduction miR- AI806395 ALFY ALFY P + T binding|zinc ion binding 155 miR- NM_000038 APC adenomatosis polyposis P + T Wnt receptor signaling pathway|beta-catenin 155 coli binding|cell adhesion|microtubule binding|negative regulation of cell cycle|protein complex assembly|signal transduction miR- NM_017610 ARK Arkadia P + T protein ubiquitination|ubiquitin ligase 155 complex|ubiquitin-protein ligase activity|zinc ion binding miR- BG032269 ARL8 ADP-ribosylation-like M + P + T GTP binding|small GTPase mediated signal 155 factor 8 transduction miR- AB000815 ARNTL aryl hydrocarbon P + T circadian rhythm|nucleus|regulation of transcription, 155 receptor nuclear DNA-dependent|signal transducer activity|signal translocator-like transduction|transcription|transcription factor activity miR- NM_001670 ARVCF armadillo repeat gene P + T cell adhesion|cytoskeleton|development|protein 155 deletes in binding|structural molecule activity velocardiofacial syndrome miR- AK024064 ASTN2 astrotactin 2 P + T integral to membrane 155 miR- M95541 ATP2B1 ATPase, Ca++ M + P + T ATP binding|calcium ion binding|calcium ion 155 transporting, plasma transport|calcium-transporting ATPase membrane 1 activity|calmodulin binding|cation transport|hydrolase activity|hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances|integral to plasma membrane|magnesium ion binding|membrane|metabolism miR- NM_001186 BACH1 BTB and CNC P + T DNA binding|nucleus|protein binding|regulation of 155 homology 1, basic transcription, DNA- leucine zipper dependent|transcription|transcription factor activity transcription factor 1 miR- NM_007005 BCE-1 BCE-1 protein P + T frizzled signaling pathway|molecular_function 155 unknown|nucleus|nucleus|regulation of transcription|regulation of transcription, DNA- dependent miR- NM_022893 BCL11A B-cell CLL/lymphoma P + T cytoplasm|hemopoiesis|nucleic acid 155 11A (zinc finger binding|nucleus|nucleus|regulation of transcription, protein) DNA-dependent|transcription|zinc ion binding miR- NM_001709 BDNF brain-derived M + T growth factor activity|growth factor 155 neurotrophic factor activity|neurogenesis miR- NM_014577 BRD1 bromodomain P + T DNA binding|cell cycle|nucleus|nucleus|regulation of 155 containing 1 transcription, DNA-dependent miR- NM_024529 C1orf28 chromosome 1 open M + P + T 155 reading frame 28 miR- NM_000719 CACNA1C calcium channel, P + T calcium ion binding|calcium ion transport|cation 155 voltage-dependent, L transport|integral to membrane|ion channel type, alpha 1C subunit activity|ion transport|membrane|regulation of heart contraction rate|voltage-gated calcium channel activity|voltage-gated calcium channel activity|voltage-gated calcium channel complex|voltage-gated calcium channel complex miR- AL118798 CD47 CD47 antigen (Rh- P + T cell-matrix adhesion|integral to plasma 155 related antigen, membrane|integrin-mediated signaling integrin-associated pathway|plasma membrane|protein binding signal transducer) miR- AL564683 CEBPB CCAAT/enhancer M + P + T acute-phase response|inflammatory 155 binding protein response|nucleus|regulation of transcription, DNA- (C/EBP), beta dependent|transcription|transcription factor activity|transcription from RNA polymerase II
promoter miR- NM_007023 CGEF2 cAMP-regulated M + P 3',5'-cAMP binding|G-protein coupled receptor 155 guanine nucleotide protein signaling pathway|cAMP-dependent protein exchange factor II kinase complex|cAMP-dependent protein kinase regulator activity|exocytosis|guanyl-nucleotide exchange factor activity|membrane fraction|nucleotide binding|protein amino acid phosphorylation|small GTPase mediated signal transduction miR- AU152178 CMG2 capillary P + T integral to membrane|receptor activity 155 morphogenesis protein 2 miR- NM_005776 CNIH cornichon homolog P + T immune response|integral to membrane|intracellular 155 (Drosophila) signaling cascade|membrane miR- AW241703 CNTN4 Homo sapiens cDNA P + T cell adhesion|membrane|protein binding 155 FLJ32716 fis, clone TESTI2000808, highly similar to Rattus norvegicus neural cell adhesion protein BIG-2 precursor (BIG-2) mRNA, mRNA sequence miR- NM_000094 COL7A1 collagen, type VII, P + T basement membrane|cell adhesion|collagen type 155 alpha 1 (epidermolysis VII|cytoplasm|epidermis development|phosphate bullosa, dystrophic, transport|protein binding|serine-type endopeptidase dominant and recessive) inhibitor activity|structural molecule activity miR- NM_003653 COPS3 COP9 constitutive P + T signalosome complex 155 photomorphogenic homolog subunit 3 (Arabidopsis) miR- NM_005211 CSF1R colony stimulating M + P + T ATP binding|antimicrobial humoral response (sensu 155 factor 1 receptor, Vertebrata)|cell proliferation|development|integral to formerly McDonough plasma membrane|macrophage colony stimulating feline sarcoma viral (v- factor receptor activity|plasma membrane|protein fms) oncogene homolog amino acid phosphorylation|receptor activity|signal transduction|transferase activity|transmembrane receptor protein tyrosine kinase signaling pathway miR- NM_001892 CSNK1A1 casein kinase 1, alpha 1 P + T ATP binding|Wnt receptor signaling pathway|casein 155 kinase I activity|protein amino acid phosphorylation|protein amino acid phosphorylation|protein serine/threonine kinase activity|protein-tyrosine kinase activity|transferase activity miR- NM_005214 CTLA4 cytotoxic T- P + T immune response|immune response|integral to plasma 155 lymphocyte-associated membrane|membrane protein 4 miR- U69546 CUGBP2 CUG triplet repeat, M + P + T RNA binding|RNA binding|RNA 155 RNA binding protein 2 processing|neuromuscular junction development|nucleotide binding|regulation of heart contraction rate miR- NM_030927 DC- tetraspanin similar to P + T integral to membrane 155 TM4F2 TM4SF9 miR- NM_015652 DKFZP564P1916 DKFZP564P1916 P + T 155 protein miR- AF151831 DKFZP566C134 DKFZP566C134 P + T protein binding 155 protein miR- NM_004411 DNCI1 dynein, cytoplasmic, P + T cytoplasmic dynein complex|motor activity 155 intermediate polypeptide 1 miR- NM_001400 EDG1 endothelial P + T G-protein coupled receptor protein signaling 155 differentiation, pathway|cell adhesion|integral to plasma sphingolipid G-protein- membrane|lysosphingolipid and lysophosphatidic acid coupled receptor, 1 receptor activity|plasma membrane|receptor activity|signal transduction miR- NM_006795 EHD1 EH-domain containing 1 P + T ATP binding|GTP binding|GTPase 155 activity|biological_process unknown|calcium ion binding|cellular_component unknown miR- NM_012081 ELL2 ELL-related RNA M + P + T RNA elongation from RNA polymerase II 155 polymerase II, promoter|RNA polymerase II transcription factor elongation factor activity|nucleus|regulation of transcription, DNA- dependent|transcription|transcription elongation factor complex miR- NM_005238 ETS1 v-ets erythroblastosis P + T RNA polymerase II transcription factor 155 virus E26 oncogene activity|immune response|negative regulation of cell homolog 1 (avian) proliferation|nucleus|regulation of transcription, DNA-dependent|transcription|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_002009 FGF7 fibroblast growth factor P + T cell proliferation|cell-cell signaling|epidermis 155 7 (keratinocyte growth development|extracellular region|growth factor factor) activity|positive regulation of cell proliferation|regulation of cell cycle|response to wounding|signal transduction miR- NM_018208 FLJ10761 hypothetical protein P + T biological_process unknown|cellular_component 155 FLJ10761 unknown|choline kinase activity|transferase activity miR- NM_018243 FLJ10849 hypothetical protein P + T GTP binding|cell cycle|cytokinesis 155 FLJ10849 miR- NM_022064 FLJ12565 hypothetical protein P + T ligase activity|protein ubiquitination|ubiquitin ligase 155 FLJ12565 complex|ubiquitin-protein ligase activity|zinc ion binding miR- NM_018391 FLJ23277 FLJ23277 protein P + T 155 miR- NM_021078 GCN5L2 GCN5 general control M + P + T N-acetyltransferase activity|chromatin 155 of amino-acid synthesis remodeling|histone acetyltransferase activity|histone 5-like 2 (yeast) deacetylase binding|nucleus|protein amino acid acetylation|regulation of transcription from RNA polymerase II promoter|transcription|transcription coactivator activity|transferase activity miR- NM_018178 GPP34R hypothetical protein P + T 155 FLJ10687 miR- AF019214 HBP1 HMG-box containing M + P DNA binding|nucleus|regulation of transcription, 155 protein 1 DNA-dependent miR- NM_006037 HDAC4 histone deacetylase 4 P + T B-cell differentiation|cell cycle|chromatin 155 modification|cytoplasm|development|histone deacetylase activity|histone deacetylase complex|hydrolase activity|inflammatory response|negative regulation of myogenesis|neurogenesis|nucleus|regulation of transcription, DNA- dependent|transcription|transcription factor binding|transcriptional repressor activity miR- NM_001530 HIF1A hypoxia-inducible P + T RNA polymerase II transcription factor activity, 155 factor 1, alpha subunit enhancer binding|electron transport|histone (basic helix-loop-helix acetyltransferase transcription factor) binding|homeostasis|nucleus|nucleus|protein heterodimerization activity|protein heterodimerization activity|regulation of transcription, DNA- dependent|response to hypoxia|signal transducer activity|signal transduction|signal transduction|transcription factor activity miR- AL023584 HIVEP2 human P + T 155 immunodeficiency virus type I enhancer binding protein 2 miR- AI682088 HLCS holocarboxylase P + T biotin-[acetyl-CoA-carboxylase] ligase activity|biotin- 155 synthetase (biotin- [methylcrotonoyl-CoA-carboxylase] ligase [proprionyl-Coenzyme activity|biotin-[methylmalonyl-CoA- A-carboxylase (ATP- carboxytransferase] ligase activity|biotin-[propionyl- hydrolysing)] ligase) CoA-carboxylase (ATP-hydrolyzing)] ligase activity|ligase activity|protein modification miR- NM_020190 HNOEL- HNOEL-iso protein P + T 155 iso miR- NM_014002 IKBKE inhibitor of kappa light P + T ATP binding|NF-kappaB-inducing kinase 155 polypeptide gene activity|cytoplasm|immune response|positive enhancer in B-cells, regulation of I-kappaB kinase/NF-kappaB kinase epsilon cascade|protein amino acid phosphorylation|protein serine/threonine kinase activity|signal transducer activity|transferase activity miR- D13720 ITK IL2-inducible T-cell P + T ATP binding|cellular defense response|intracellular 155 kinase signaling cascade|non-membrane spanning protein tyrosine kinase activity|protein amino acid phosphorylation|transferase activity miR- NM_002249 KCNN3 potassium P + T calcium-activated potassium channel activity|calcium- 155 intermediate/small activated potassium channel activity|calmodulin conductance calcium- binding|integral to membrane|ion channel activity|ion activated channel, transport|membrane|membrane subfamily N, member 3 fraction|neurogenesis|potassium ion transport|potassium ion transport|small conductance calcium-activated potassium channel activity|synaptic transmission|voltage-gated potassium channel complex miR- AB033100 KIAA1274 KIAA protein (similar P + T protein tyrosine phosphatase activity 155 to mouse paladin) miR- NM_017780 KIAA1416 KIAA1416 protein P + T ATP binding|chromatin|chromatin assembly or 155 disassembly|chromatin binding|helicase activity|nucleus miR- NM_002264 KPNA1 karyopherin alpha 1 P + T NLS-bearing substrate-nucleus 155 (importin alpha 5) import|cytoplasm|intracellular protein transport|nuclear localization sequence binding|nuclear pore|nucleus|protein binding|protein transporter activity|regulation of DNA recombination miR- AK021602 KPNA4 karyopherin alpha 4 P + T NLS-bearing substrate-nucleus 155 (importin alpha 3) import|binding|intracellular protein transport|nucleus|protein transporter activity miR- NM_020354 LALP1 lysosomal apyrase-like M + P + T hydrolase activity 155 protein 1 miR- AW242408 LOC151531 Similar to uridine M + P + T cytosol|nucleoside metabolism|nucleotide 155 phosphorylase [Homo catabolism|protein binding|transferase activity, sapiens], mRNA transferring glycosyl groups|type III intermediate sequence filament|uridine metabolism|uridine phosphorylase activity miR- NM_016210 LOC51161 g20 protein P + T 155 miR- NM_018557 LRP1B low density lipoprotein- P + T calcium ion binding|integral to membrane|low-density 155 related protein 1B lipoprotein receptor activity|membrane|protein (deleted in tumors) transport|receptor activity|receptor mediated endocytosis miR- NM_002446 MAP3K10 mitogen-activated M + P + T ATP binding|JUN kinase kinase kinase 155 protein kinase kinase activity|activation of kinase 10 JNK|autophosphorylation|induction of apoptosis|protein homodimerization activity|protein serine/threonine kinase activity|protein-tyrosine kinase activity|signal transduction|transferase activity miR- NM_003954 MAP3K14 mitogen-activated P + T ATP binding|protein amino acid 155 protein kinase kinase phosphorylation|protein serine/threonine kinase kinase 14 activity|transferase activity miR- AL117407 MAP3K7IP2 mitogen-activated P + T kinase activity|positive regulation of I-kappaB 155 protein kinase kinase kinase/NF-kappaB cascade|positive regulation of I- kinase 7 interacting kappaB kinase/NF-kappaB cascade|signal transducer protein 2 activity|signal transducer activity miR- NM_004992 MECP2 methyl CpG binding M + P + T DNA binding|negative regulation of transcription 155 protein 2 (Rett from RNA polymerase II promoter|nucleus|regulation syndrome) of transcription, DNA- dependent|transcription|transcription corepressor activity miR- NM_002398 MEIS1 Meis1, myeloid M + P + T RNA polymerase II transcription factor 155 ecotropic viral activity|nucleus|regulation of transcription, DNA- integration site 1 dependent|transcription factor activity
homolog (mouse) miR- NM_016289 MO25 MO25 protein P + T 155 miR- AA621962 MYO1D myosin ID M + P + T ATP bindinglactin binding|calmodulin binding|motor 155 activity|myosin miR- NM_030571 N4WBP5 likely ortholog of P + T positive regulation of I-kappaB kinase/NF-kappaB 155 mouse Nedd4 WW cascade|signal transducer activity binding protein 5 miR- NM_014903 NAV3 neuron navigator 3 P + T ATP binding|mitochondrion|nucleoside- 155 triphosphatase activity|nucleotide binding miR- NM_030571 NDFIP1 likely ortholog of P + T positive regulation of I-kappaB kinase/NF-kappaB 155 mouse Nedd4 WW cascade|signal transducer activity binding protein 5 miR- NM_006599 NFAT5 nuclear factor of M + P + T RNA polymerase II transcription factor 155 activated T-cells 5, activity|excretion|nucleus|regulation of transcription, tonicity-responsive DNA-dependent|signal transduction|transcription factor activity|transcription from RNA polymerase II promoter miR- NM_002515 NOVA1 neuro-oncological M + P + T RNA binding|RNA binding|RNA splicing|RNA 155 ventral antigen 1 splicing|locomotory behavior|locomotory behavior|nucleus|synaptic transmission|synaptic transmission miR- AI373299 PANK1 pantothenate kinase 1 P + T ATP binding|coenzyme A biosynthesis|pantothenate 155 kinase activity|transferase activity miR- BG110231 PAPOLA poly(A) polymerase P + T RNA binding|cytoplasm|mRNA 155 alpha polyadenylylation|mRNA processing|nucleus|polynucleotide adenylyltransferase activity|transcription|transferase activity miR- NM_020403 PCDH9 protocadherin 9 M + P + T calcium ion binding|cell adhesion|homophilic cell 155 adhesion|integral to membrane|membrane|protein binding miR- NM_002655 PLAG1 pleiomorphic adenoma P + T nucleic acid binding|nucleus|transcription factor 155 gene 1 activity|zinc ion binding miR- AJ272212 PSKH1 protein serine kinase H1 P + T ATP binding|Golgi apparatus|nucleus|protein amino 155 acid phosphorylation|protein serine/threonine kinase activity|transferase activity miR- NM_014904 Rab11- KIAA0941 protein P + T 155 FIP2 miR- AF322067 RAB34 RAB34, member RAS P + T GTP binding|Golgi apparatus|protein transport|small 155 oncogene family GTPase mediated signal transduction miR- NM_002869 RAB6A RAB6A, member RAS M + P + T GTP binding|GTPase activity|Golgi apparatus|protein 155 oncogene family transport|small GTPase mediated signal transduction miR- AL136727 RAB6C RAB6C, member RAS M + P + T GTP binding|GTPase activity|intracellular|protein 155 oncogene family transport|response to drug|small GTPase mediated signal transduction miR- NM_002902 RCN2 reticulocalbin 2, EF- P + T calcium ion binding|endoplasmic reticulum|protein 155 hand calcium binding binding domain miR- AJ223321 RP58 zinc finger protein 238 M + P + T 155 miR- NM_002968 SALL1 sal-like 1 (Drosophila) P + T morphogenesis|nucleus|regulation of transcription, 155 DNA-dependent|transcription|transcription factor activity|zinc ion binding miR- NM_002971 SATB1 special AT-rich P + T double-stranded DNA binding|establishment and/or 155 sequence binding maintenance of chromatin protein 1 (binds to architecture|nucleus|regulation of transcription, DNA- nuclear matrix/scaffold- dependent|transcription factor activity associating DNA's) miR- NM_003469 SCG2 secretogranin II P + T calcium ion binding|protein secretion 155 (chromogranin C) miR- NM_005625 SDCBP syndecan binding P + T actin cytoskeleton organization and 155 protein (syntenin) biogenesis|adherens junction|cytoskeletal adaptor activity|cytoskeleton|endoplasmic reticulum|interleukin-5 receptor binding|interleukin-5 receptor complex|intracellular signaling cascade|metabolism|neurexin binding|nucleus|oxidoreductase activity|plasma membrane|protein binding|protein heterodimerization activity|protein-membrane targeting|substrate-bound cell migration, cell extension|synaptic transmission|syndecan binding miR- NM_000232 SGCB sarcoglycan, beta P + T cytoskeleton|cytoskeleton organization and 155 (43 kDa dystrophin- biogenesis|integral to plasma membrane|muscle associated glycoprotein) development|sarcoglycan complex miR- NM_013257 SGKL serum/glucocorticoid P + T ATP binding|intracellular signaling cascade|protein 155 regulated kinase-like amino acid phosphorylation|protein amino acid phosphorylation|protein serine/threonine kinase activity|protein serine/threonine kinase activity|protein-tyrosine kinase activity|response to stress|transferase activity miR- NM_005069 SIM2 single-minded homolog P + T cell differentiation|neurogenesis|nucleus|regulation of 155 2 (Drosophila) transcription, DNA-dependent|signal transducer activity|signal transduction|transcription|transcription factor activity miR- AA927480 SKI v-ski sarcoma viral P + T 155 oncogene homolog (avian) miR- NM_006748 SLA Src-like-adaptor P + T SH3/SH2 adaptor activity|intracellular signaling 155 cascade miR- AI684141 SMARCA4 SWI/SNF related, P + T ATP binding|DNA binding|helicase activity|helicase 155 matrix associated, actin activity|hydrolase dependent regulator of activity|nucleoplasm|nucleus|regulation of chromatin, subfamily a, transcription from RNA polymerase II member 4 promoter|transcription|transcription coactivator activity|transcription factor activity miR- AB005043 SOCS1 suppressor of cytokine M + P + T JAK-STAT cascade|cytoplasm|insulin-like growth 155 signaling 1 factor receptor binding|intracellular signaling cascade|negative regulation of JAK-STAT cascade|protein kinase binding|protein kinase inhibitor activity|regulation of cell growth|ubiquitin cycle miR- NM_004232 SOCS4 suppressor of cytokine M + P JAK-STAT cascade|cytoplasm|defense 155 signaling 4 response|intracellular signaling cascade|regulation of cell growth miR- NM_005986 SOX1 SRY (sex determining P + T DNA binding|establishment and/or maintenance of 155 region Y)-box 1 chromatin architecture|nucleus|regulation of transcription, DNA-dependent|regulation of transcription, DNA-dependent|transcription factor activity miR- AI360875 SOX11 SRY (sex determining M + T DNA binding|neurogenesis|nucleus|regulation of 155 region Y)-box 11 transcription, DNA-dependent|transcription miR- AL136780 SOX6 SRY (sex determining P + T establishment and/or maintenance of chromatin 155 region Y)-box 6 architecture|heart development|muscle development|nucleus|regulation of transcription, DNA-dependent|transcription|transcription factor activity miR- AW470841 SP3 Sp3 transcription factor P + T DNA binding|nucleus|regulation of transcription, 155 DNA-dependent|transcription|transcriptional activator activity|transcriptional repressor activity|zinc ion binding miR- BF224259 SPF30 splicing factor 30, P + T RNA splicing|RNA splicing factor activity, 155 survival of motor transesterification neuron-related mechanism|apoptosis|cytoplasm|induction of apoptosis|spliceosome assembly|spliceosome complex miR- NM_003120 SPI1 spleen focus forming M + T negative regulation of transcription from RNA 155 virus (SFFV) proviral polymerase II promoter|nucleus|regulation of integration oncogene transcription, DNA- spi1 dependent|transcription|transcription factor activity miR- BE676214 SSH2 slingshot 2 P + T protein amino acid dephosphorylation|protein 155 tyrosine/serine/threonine phosphatase activity miR- AF159447 SUFU suppressor of fused P + T cell cycle|cytoplasm|development|negative regulation 155 homolog (Drosophila) of cell cycle|nucleus|proteolysis and peptidolysis|signal transducer activity|signal transduction|skeletal development|transcription corepressor activity miR- NM_006754 SYPL synaptophysin-like M + P + T integral to plasma membrane|membrane|synaptic 155 protein transmission|synaptic vesicle|transport|transporter activity miR- NM_006286 TFDP2 transcription factor Dp- P + T DNA metabolism|nucleus|regulation of cell 155 2 (E2F dimerization cycle|regulation of transcription from RNA partner 2) polymerase II promoter|transcription|transcription cofactor activity|transcription factor activity|transcription factor complex miR- AA705845 TLE4 transducin-like P + T frizzled signaling pathway|molecular_function 155 enhancer of split 4 unknown|nucleus|nucleus|regulation of (E(sp1) homolog, transcription|regulation of transcription, DNA- Drosophila) dependent miR- NM_014765 TOMM20 translocase of outer P + T integral to membrane|mitochondrial outer membrane 155 mitochondrial translocase complex|mitochondrion|outer membrane 20 (yeast) membrane|protein translocase activity|protein- homolog mitochondrial targeting miR- AW341649 TP53INP1 tumor protein p53 P + T apoptosis|nucleus 155 inducible nuclear protein 1 miR- BC005016 TRIM2 tripartite motif- P + T cytoplasm|myosin binding|protein 155 containing 2 ubiquitination|ubiquitin ligase complex|ubiquitin- protein ligase activity|zinc ion binding miR- AA524505 TSGA zinc finger protein P + T nucleus 155 miR- AW157525 TSGA14 testis specific, 14 M + P + T centrosome 155 miR- X62048 WEE1 WEE1 homolog (S. pombe) P + T ATP binding|cytokinesis|mitosis|nucleus|protein 155 amino acid phosphorylation|protein serine/threonine kinase activity|protein-tyrosine kinase activity|regulation of cell cycle|transferase activity miR- AC005539 WUGSC:H_NH0335J18.1 Similar to uridine M + P + T 155 phosphorylase [Homo sapiens], mRNA sequence miR- NM_003413 ZIC3 Zic family member 3 P + T DNA binding|determination of left/right 155 heterotaxy 1 (odd- symmetry|nucleus|regulation of transcription, DNA- paired homolog, dependent|transcription|zinc ion binding Drosophila) miR- NM_007345 ZNF236 zinc finger protein 236 P + T nucleus|regulation of transcription, DNA- 155 dependent|transcription|transcription factor activity|zinc ion binding miR- NM_006352 ZNF238 zinc finger protein 238 M + P + T chromosome organization and biogenesis (sensu 155 Eukaryota)|negative regulation of transcription from RNA polymerase II promoter|nuclear chromosome|nucleic acid binding|nucleus|protein binding|protein binding|regulation of transcription, DNA-dependent|specific RNA polymerase II transcription factor activity|transcription|transcription factor activity|transport|zinc ion binding miR-21 NM_005164 ABCD2 ATP-binding cassette, M + P ATP binding|ATP-binding cassette (ABC) transporter sub-family D (ALD), complex|ATPase activity|ATPase activity, coupled to member 2 transmembrane movement of substances|fatty acid metabolism|integral to plasma membrane|membrane|peroxisome|transport miR-21 NM_001616 ACVR2 activin A receptor, type P + T ATP binding|integral to plasma II membrane|membrane|protein amino acid phosphorylation|receptor activity|transferase activity|transforming growth factor beta receptor activity|transmembrane receptor protein serine/threonine kinase signaling pathway miR-21 NM_015339 ADNP activity-dependent P + T nucleus|regulation of transcription, DNA- neuroprotector dependent|transcription factor activity|zinc ion binding miR-21 AI990366 ARHGEF7 Rho guanine nucleotide P + T guanyl-nucleotide exchange factor activity|signal
exchange factor (GEF) 7 transduction miR-21 NM_017610 ARK Arkadia P + T protein ubiquitination|ubiquitin ligase complex|ubiquitin-protein ligase activity|zinc ion binding miR-21 NM_014034 ASF1A DKFZP547E2110 P + T chromatin binding|loss of chromatin silencing|nucleus protein miR-21 NM_017680 ASPN asporin (LRR class 1) P + T miR-21 NM_000657 BCL2 B-cell CLL/lymphoma 2 P + T anti-apoptosis|endoplasmic reticulum|humoral immune response|integral to membrane|membrane|mitochondrial outer membrane|mitochondrial outer membrane|mitochondrion|negative regulation of cell proliferation|nucleus|protein binding|regulation of apoptosis|regulation of cell cycle|release of cytochrome c from mitochondria miR-21 NM_014577 BRD1 bromodomain P + T DNA binding|cell cycle|nucleus|nucleus|regulation of containing 1 transcription, DNA-dependent miR-21 AA902767 BRD2 bromodomain P + T nucleus|protein serine/threonine kinase containing 2 activity|spermatogenesis miR-21 NM_014962 BTBD3 BTB (POZ) domain P + T protein binding containing 3 miR-21 NM_006763 BTG2 BTG family, member 2 P + T DNA repair|negative regulation of cell proliferation|regulation of transcription, DNA- dependent|transcription|transcription factor activity miR-21 AK025768 C20orf99 chromosome 20 open P + T nucleic acid binding reading frame 99 miR-21 AI671238 CAPN3 Homo sapiens cDNA P + T calcium ion binding|calpain activity|calpain FLJ23750 fis, clone activity|intracellular|intracellular|muscle HEP16527, mRNA development|proteolysis and peptidolysis|proteolysis sequence and peptidolysis|signal transducer activity miR-21 NM_002981 CCL1 chemokine (C-C motif) P + T calcium ion homeostasis|cell-cell signaling|chemokine ligand 1 activity|chemotaxis|extracellular space|inflammatory response|sensory perception|signal transduction|viral life cycle miR-21 BF939071 CCM1 cerebral cavernous M + P binding|catalytic activity|cytoskeleton|small GTPase malformations 1 mediated signal transduction|small GTPase regulator activity miR-21 NM_001789 CDC25A cell division cycle 25A AP + T cell proliferation|cytokinesis|hydrolase activity|intracellular|mitosis|protein amino acid dephosphorylation|protein tyrosine phosphatase activity|regulation of cyclin dependent protein kinase activity miR-21 NM_001842 CNTFR ciliary neurotrophic M + P + T ciliary neurotrophic factor receptor activity|cytokine factor receptor binding|extrinsic to membrane|neurogenesis|receptor activity|signal transduction miR-21 NM_001310 CREBL2 cAMP responsive P + T nucleus|regulation of transcription, DNA- element binding dependent|signal protein-like 2 transduction|transcription|transcription factor activity miR-21 NM_016441 CRIM1 cysteine-rich motor M + P + T insulin-like growth factor receptor activity|integral to neuron 1 membrane|membrane fraction|neurogenesis|serine- type endopeptidase inhibitor activity miR-21 NM_015396 DKFZP434A043 DKFZP434A043 P + T cell adhesion|cytoskeleton|mitotic chromosome protein condensation|protein binding|structural molecule activity miR-21 AL047650 DKFZp434A2417 endozepine-related P + T acyl-CoA binding protein precursor miR-21 AB028628 DKFZP547E2110 DKFZP547E2110 P + T chromatin binding|loss of chromatin silencing|nucleus protein miR-21 NM_031305 DKFZP564B1162 hypothetical protein P + T GTPase activator activity DKFZp564B1162 miR-21 NM_004405 DLX2 distal-less homeo box 2 P + T brain development|development|nucleus|regulation of transcription, DNA-dependent|transcription factor activity miR-21 NM_001949 E2F3 E2F transcription factor 3 M + P + T nucleus|protein binding|regulation of cell cycle|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcription factor complex|transcription initiation from RNA polymerase II promoter miR-21 NM_006795 EHD1 EH-domain containing 1 P + T ATP binding|GTP binding|GTPase activity|biological_process unknown|calcium ion binding|cellular_component unknown miR-21 NM_001412 EIF1A eukaryotic translation P + T RNA binding|eukaryotic translation initiation factor initiation factor 1A 4F complex|protein biosynthesis|translation initiation factor activity|translational initiation|translational initiation miR-21 AI832074 EIF2C2 eukaryotic translation P + T cellular_component unknown|protein initiation factor 2C, 2 biosynthesis|translation initiation factor activity miR-21 NM_006874 ELF2 E74-like factor 2 (ets P + T nucleus|nucleus|protein binding|protein domain transcription binding|regulation of transcription from RNA factor) polymerase II promoter|regulation of transcription, DNA-dependent|transcription factor activity|transcriptional activator activity|transcriptional activator activity miR-21 NM_004438 EPHA4 EphA4 P + T ATP binding|ephrin receptor activity|integral to plasma membrane|membrane|protein amino acid phosphorylation|receptor activity|signal transduction|transferase activity|transmembrane receptor protein tyrosine kinase signaling pathway miR-21 BE888593 FLJ11220 hypothetical protein P + T FLJ11220 miR-21 NM_017637 FLJ20043 hypothetical protein P + T nucleic acid binding|nucleus|zinc ion binding FLJ20043 miR-21 AF019214 HBP1 HMG-box containing M + P + T DNA binding|nucleus|regulation of transcription, protein 1 DNA-dependent miR-21 NM_000214 JAG1 jagged 1 (Alagille M + P + T Notch binding|Notch signaling syndrome) pathway|angiogenesis|calcium ion binding|calcium ion binding|cell communication|cell fate determination|development|endothelial cell differentiation|extracellular region|growth factor activity|hemopoiesis|integral to plasma membrane|keratinocyte differentiation|membrane|myoblast differentiation|neurogenesis|regulation of cell migration|regulation of cell proliferation|structural molecule activity miR-21 NM_002232 KCNA3 potassium voltage-gated M + P + T cation transport|delayed rectifier potassium channel channel, shaker-related activity|integral to membrane|membrane|membrane subfamily, member 3 fraction|potassium ion transport|voltage-gated potassium channel complex miR-21 NM_014766 KIAA0193 KIAA0193 gene P + T cellular_component unknown|dipeptidase product activity|exocytosis|proteolysis and peptidolysis miR-21 NM_014912 KIAA0940 KIAA0940 protein M + P + T nucleic acid binding miR-21 NM_014952 KIAA0945 KIAA0945 protein P + T DNA binding miR-21 NM_017780 KIAA1416 KIAA1416 protein P + T ATP binding|chromatin|chromatin assembly or disassembly|chromatin binding|helicase activity|nucleus miR-21 AB040901 KIAA1468 KIAA1468 protein P + T binding|mitotic chromosome condensation miR-21 U90268 Krit1 cerebral cavernous M + P binding|catalytic activity|cytoskeleton|small GTPase malformations 1 mediated signal transduction|small GTPase regulator activity miR-21 BF591611 LOC147632 hypothetical protein P + T oxidoreductase activity|zinc ion binding BC010734 miR-21 NM_005904 MADH7 MAD, mothers against P + T intracellular|protein binding|receptor signaling protein decapentaplegic serine/threonine kinase signaling protein homolog 7 (Drosophila) activity|regulation of transcription, DNA- dependent|response to stress|transcription|transforming growth factor beta receptor signaling pathway|transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity miR-21 NM_025146 MAK3P likely ortholog of P + T N-acetyltransferase activity mouse Mak3p homolog (S. cerevisiae) miR-21 NM_014319 MAN1 integral inner nuclear P + T integral to membrane|integral to nuclear inner membrane protein membrane|membrane fraction|nuclear membrane|nucleotide binding miR-21 AW025150 MAP3K12 mitogen-activated M + T ATP binding|JNK cascade|cytoplasm|magnesium ion protein kinase kinase binding|plasma membrane|protein amino acid kinase 12 phosphorylation|protein kinase cascade|protein serine/threonine kinase activity|protein-tyrosine kinase activity|transferase activity miR-21 NM_012325 MAPRE1 microtubule-associated P + T cell proliferation|cytokinesis|microtubule protein, RP/EB family, binding|mitosis|protein C-terminus binding|regulation member 1 of cell cycle miR-21 NM_002380 MATN2 matrilin 2 P + T biological_process unknown|calcium ion binding|extracellular matrix (sensu Metazoa) miR-21 NM_018834 MATR3 matrin 3 M + P + T RNA binding|nuclear inner membrane|nucleotide binding|nucleus|structural molecule activity|zinc ion binding miR-21 NM_021038 MBNL1 muscleblind-like M + P + T cytoplasm|double-stranded RNA binding|embryonic (Drosophila) development (sensu Mammalia)|embryonic limb morphogenesis|muscle development|myoblast differentiation|neurogenesis|nucleic acid binding|nucleus|nucleus miR-21 AI139252 MGC16063 ribosomal protein L35a P + T JAK-STAT cascadelacute-phase response|calcium ion binding|cell motility|cytoplasm|hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity|intracellular signaling cascade|negative regulation of transcription from RNA polymerase II promoter|neurogenesis|nucleus|nucleus|regulation of transcription, DNA-dependent|signal transducer activity|transcription|transcription factor activity|transcription factor activity miR-21 BC004162 MGC2452 hypothetical protein P + T fatty acid metabolism|generation of precursor MGC2452 metabolites and energy|ligand-dependent nuclear receptor activity|lipid metabolism|nucleus|nucleus|regulation of transcription, DNA-dependent|steroid hormone receptor activity|transcription|transcription factor activity|transcription factor activity|transcription from RNA polymerase II promoter miR-21 NM_024052 MGC3048 hypothetical protein P + T MGC3048 miR-21 AB049636 MRPL9 mitochondrial P + T mitochondrion|protein ribosomal protein L9 biosynthesis|ribosome|structural constituent of ribosome miR-21 NM_015678 NBEA neurobeachin P + T Golgi trans face|cytosol|endomembrane system|plasma membrane|post-Golgi transport|postsynaptic membrane|protein kinase A binding miR-21 AI700518 NFIB nuclear factor I/B M + T DNA replication|nucleus|nucleus|regulation of transcription, DNA- dependent|transcription|transcription factor activity|transcription factor activity miR-21 NM_002527 NTF3 neurotrophin 3 M + P anti-apoptosis|cell motility|cell-cell signaling|growth factor activity|neurogenesis|signal transduction miR-21 U24223 PCBP1 poly(rC) binding M + P + T RNA binding|catalytic activity|cytoplasm|mRNA protein 1 metabolism|nucleus|ribonucleoprotein complex|single-stranded DNA binding miR-21 NM_005016 PCBP2 poly(rC) binding M + T DNA binding|RNA binding|cytoplasm|mRNA protein 2 metabolism|nucleic acid binding|nucleus|ribonucleoprotein complex miR-21 NM_014456 PDCD4 programmed cell death P + T apoptosis 4 (neoplastic transformation inhibitor) miR-21 AF338650 PDZD2 PDZ domain containing 2 P + T miR-21 NM_000325 PITX2 paired-like M + P + T determination of left/right
homeodomain symmetry|development|nucleus|organogenesis| transcription factor 2 regulation of transcription, DNA-dependent| transcription factor activity miR-21 NM_002655 PLAG1 pleiomorphic adenoma P + T nucleic acid binding|nucleus|transcription factor gene 1 activity|zinc ion binding miR-21 NM_005036 PPARA peroxisome P + T fatty acid metabolism|generation of precursor proliferative activated metabolites and energy|ligand-dependent nuclear receptor, alpha receptor activity|lipid metabolism|nucleus|nucleus|regulation of transcription, DNA-dependent|steroid hormone receptor activity|transcription|transcription factor activity|transcription factor activity|transcription from RNA polymerase II promoter miR-21 NM_002711 PPP1R3A protein phosphatase 1, P + T carbohydrate metabolism|glycogen regulatory (inhibitor) metabolism|hydrolase activity|integral to subunit 3A (glycogen membrane|phosphoprotein phosphatase activity|type 1 and sarcoplasmic serine/threonine specific protein phosphatase inhibitor reticulum binding activity subunit, skeletal muscle) miR-21 NM_000944 PPP3CA protein phosphatase 3 P + T calcineurin complex|calcium ion binding|calmodulin (formerly 2B), catalytic binding|hydrolase activity|protein amino acid subunit, alpha isoform dephosphorylation|protein serine/threonine (calcineurin A alpha) phosphatase activity miR-21 NM_018569 PRO0971 hypothetical protein P + T PRO0971 miR-21 AA156948 PRPF4B PRP4 pre-mRNA M + T ATP binding|RNA splicing|nuclear mRNA splicing, processing factor 4 via spliceosome|nucleus|protein amino acid homolog B (yeast) phosphorylation|protein serine/threonine kinase activity|transferase activity miR-21 BF337790 PURB purine-rich element M + P + T binding protein B miR-21 NM_002869 RAB6A RAB6A, member RAS P + T GTP binding|GTPase activity|Golgi apparatus|protein oncogene family transport|small GTPase mediated signal transduction miR-21 AL136727 RAB6C RAB6C, member RAS P + T GTP binding|GTPase activity|intracellular|protein oncogene family transport|response to drug|small GTPase mediated signal transduction miR-21 NM_002890 RASA1 RAS p21 protein P + T Ras GTPase activator activity|intracellular signaling activator (GTPase cascade activating protein) 1 miR-21 NM_005739 RASGRP1 RAS guanyl releasing P + T Ras guanyl-nucleotide exchange factor activity|Ras protein 1 (calcium and protein signal transduction|calcium ion DAG-regulated) binding|calcium ion binding|diacylglycerol binding|guanyl-nucleotide exchange factor activity|membrane fraction|small GTPase mediated signal transduction miR-21 NM_021111 RECK reversion-inducing- M + P + T cell cycle|membrane|membrane cysteine-rich protein fraction|metalloendopeptidase inhibitor with kazal motifs activity|negative regulation of cell cycle|serine-type endopeptidase inhibitor activity miR-21 NM_006915 RP2 retinitis pigmentosa 2 P + T beta-tubulin folding|membrane|sensory (X-linked recessive) perception|unfolded protein binding|visual perception miR-21 AA906056 RPS6KA3 ribosomal protein S6 M + T ATP binding|central nervous system kinase, 90 kDa, development|protein amino acid polypeptide 3 phosphorylation|protein serine/threonine kinase activity|signal transduction|skeletal development|transferase activity miR-21 NM_002971 SATB1 special AT-rich M + P + T double-stranded DNA binding|establishment and/or sequence binding maintenance of chromatin protein 1 (binds to architecture|nucleus|regulation of transcription, DNA- nuclear matrix/scaffold- dependent|transcription factor activity associating DNA's) miR-21 NM_014191 SCN8A sodium channel, voltage M + P + T ATP binding|cation channel activity|cation gated, type VIII, alpha transport|integral to polypeptide membrane|membrane|neurogenesis|sodium ion transport|voltage-gated sodium channel activity|voltage-gated sodium channel complex miR-21 AA927480 SKI v-ski sarcoma viral M + P + T oncogene homolog (avian) miR-21 NM_003983 SLC7A6 solute carrier family 7 P + T amino acid metabolism|amino acid transport|amino (cationic amino acid acid-polyamine transporter activity|integral to plasma transporter, y+ system), membrane|plasma membrane|protein complex member 6 assembly|transport miR-21 NM_006359 SLC9A6 solute carrier family 9 P + T antiporter activity|endoplasmic reticulum (sodium/hydrogen membrane|integral to membrane|integral to exchanger), isoform 6 membrane|ion transport|microsome|mitochondrion|regulation of pH|sodium ion transport|sodium:hydrogen antiporter activity|solute:hydrogen antiporter activity miR-21 NM_003076 SMARCD1 SWI/SNF related, P + T chromatin remodeling|chromatin remodeling matrix associated, actin complex|regulation of transcription from RNA dependent regulator of polymerase II promoter|transcription coactivator chromatin, subfamily d, activity member 1 miR-21 AI669815 SOX2 SRY (sex determining P + T establishment and/or maintenance of chromatin region Y)-box 2 architecture|nucleus|regulation of transcription, DNA- dependent|transcription|transcription factor activity miR-21 NM_006940 SOX5 SRY (sex determining P + T nucleus|regulation of transcription, DNA- region Y)-box 5 dependent|transcription|transcription factor activity|transcription from RNA polymerase II promoter miR-21 AI808807 SOX7 SRY (sex determining P + T DNA binding|nucleus|regulation of transcription, region Y)-box 7 DNA-dependent|transcription miR-21 NM_006717 SPIN Spindling P + T gametogenesis|ribonucleoprotein complex miR-21 NM_005842 SPRY2 sprouty homolog 2 P + T cell-cell (Drosophila) signaling|development|membrane|organogenesis| regulation of signal transduction miR-21 NM_006751 SSFA2 sperm specific antigen 2 P + T plasma membrane miR-21 NM_006603 STAG2 stromal antigen 2 P + T cell cycle|chromosome segregation|cytokinesis|meiosis|mitosis| molecular_function unknown|nucleus miR-21 BC000627 STAT3 signal transducer and P + T JAK-STAT cascade|acute-phase response|calcium ion activator of binding|cell transcription 3 (acute- motility|cytoplasm|hematopoietin/interferon-class phase response factor) (D200-domain) cytokine receptor signal transducer activity|intracellular signaling cascade|negative regulation of transcription from RNA polymerase II promoter|neurogenesis|nucleus|nucleus|regulation of transcription, DNA-dependent|signal transducer activity|transcription|transcription factor activity|transcription factor activity miR-21 AW138827 TAF5 TAF5 RNA polymerase P + T nucleus|regulation of transcription, DNA- II, TATA box binding dependent|transcription factor TFIID protein (TBP)- complex|transcription factor activity associated factor, 100 kDa miR-21 BF591040 TAGAP T-cell activation P + T GTPase activator activity GTPase activating protein miR-21 NM_000358 TGFBI transforming growth M + P + T cell adhesion|cell proliferation|extracellular matrix factor, beta-induced, (sensu Metazoa)|extracellular space|integrin 68 kDa binding|negative regulation of cell adhesion|protein binding|sensory perception|visual perception miR-21 NM_000362 TIMP3 tissue inhibitor of P + T enzyme inhibitor activity|extracellular matrix (sensu metalloproteinase 3 Metazoa)|extracellular matrix (sensu (Sorsby fundus Metazoa)|induction of apoptosis by extracellular dystrophy, signals|metalloendopeptidase inhibitor pseudoinflammatory) activity|sensory perception|visual perception miR-21 AA149745 TRIM2 tripartite motif- M + P + T cytoplasm|myosin binding|protein containing 2 ubiquitination|ubiquitin ligase complex|ubiquitin- protein ligase activity|zinc ion binding miR-21 AF346629 TRPM7 transient receptor P + T ATP binding|calcium channel activity|calcium ion potential cation transport|cation transport|integral to channel, subfamily M, membrane|membrane|protein amino acid member 7 phosphorylation|protein serine/threonine kinase activity|transferase activity miR-21 AI745185 YAP1 Yes-associated protein P + T 1, 65 kDa miR-21 NM_005667 ZFP103 zinc finger protein 103 P + T central nervous system development|integral to homolog (mouse) membrane|protein ubiquitination|ubiquitin ligase complex|ubiquitin-protein ligase activity|zinc ion binding miR-21 N62196 ZNF367 zinc finger protein 367 M + P + T nucleic acid binding|nucleus|zinc ion binding M = MiRanda P = PicTar T = TargetScan
Example 3
Bio-Pathological Features and microRNA Expression
[0143] Materials and Methods
[0144] Immunohistochemical Analysis of Breast Cancer Samples.
[0145] Staining procedures were performed as described (Querzoli, P., et al., Anal. Quant. Cytol. Histol. 21:151-160 (1999)). Hormonal receptors were evaluated with 6F11 antibody for estrogen receptor α (ER) and PGR-1A6 antibody for progesterone receptor (PR) (Ventana, Tucson, Ariz., U.S.A.). The proliferation index was assessed with MIB1 antibody (DAKO, Copenhagen). ERBB2 was detected with CB11 antibody (Ventana, Tucson, Ariz., U.S.A.) and p53 protein expression was examined with DO7 antibody (Ventana, Tucson, Ariz., U.S.A.). Only tumor cells with distinct nuclear immunostaining for ER, PR, Mib1 and p53 were recorded as positive. Tumor cells were considered positive for ERBB2 when they showed distinct membrane immunoreactivity.
[0146] To perform a quantitative analysis of the expression of these various biological markers, the Eureka Menarini computerized image analysis system was used. For each tumor section, at least 20 microscopic fields of invasive carcinoma were measured using a 40× objective. The following cut-off values were employed: 10% of positive nuclear area for ER, PR, c-erbB2 and p53, 13% of nuclei expressing Mib1 was introduced to discriminate cases with high and low proliferative activity.
[0147] Results
[0148] To evaluate whether a correlation exists between various bio-pathological features associated with breast cancer and the expression of particular miRNAs, we generated and compared miRNA expression profiles for various cancer samples associated with the presence or absence of a particular breast cancer feature. In particular, we analyzed breast cancers with lobular or ductal histotypes, breast cancers with differential expression of either estrogen receptor alpha (ER) or progesterone receptor, and breast cancers with differences in lymph node metastasis, vascular invasion, proliferation index, and expression of ERBB2 and p53.
[0149] Expression profiles of lobular or ductal and +/-ERBB2 expression classes did not reveal any microRNAs that were differentially-expressed, while all other comparisons revealed a small number of differentially-expressed microRNAs (P<0.05). The results of this analysis are shown in FIG. 4.
[0150] Differentially-expressed miRNA families were identified for various bio-pathological features that are associated with human breast cancer. For example, all miR-30 miRNAs are down-regulated in both ER- and PR-tumors, suggesting that expression of miR-30 miRNAs is regulated by these hormones. In addition, the expression of various let-7 miRNAs was down-regulated in breast cancer samples with either lymph node metastasis or a high proliferation index, suggesting that reduced let-7 expression could be associated with a poor prognosis, a result that is consistent with previous findings. The discovery that the let-7 family of miRNAs regulates the expression of members of the RAS oncogene family provides a potential explanation for the role of let-7 miRNAs in human cancer.
[0151] miR-145 and miR-21, two miRNAs whose expression could differentiate cancer or normal tissues, were also differentially-expressed in cancers with a different proliferation index or different tumor stage. In particular, miR-145 is progressively down-regulated from normal breast to cancers with a high proliferation index. Similarly, miR-21 is progressively up-regulated from normal breast to cancers with high tumor stage. These findings suggest that deregulation of these two miRNAs may affect critical molecular events involved in tumor progression.
[0152] Another miRNA potentially involved in cancer progression is miR-9-3. miR-9-3 was downregulated in breast cancers with either high vascular invasion or lymph node metastasis, suggesting that its down-regulation was acquired during the course of tumor progression and, in particular, during the acquisition of metastatic potential.
[0153] The relevant teachings of all publications cited herein that have not explicitly been incorporated by reference, are incorporated herein by reference in their entirety. While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims
Sequence CWU
1
1
290190DNAHomo sapiens 1cactgtggga tgaggtagta ggttgtatag ttttagggtc
acacccacca ctgggagata 60actatacaat ctactgtctt tcctaacgtg
90272DNAHomo sapiens 2aggttgaggt agtaggttgt
atagtttaga attacatcaa gggagataac tgtacagcct 60cctagctttc ct
72374DNAHomo sapiens
3gggtgaggta gtaggttgta tagtttgggg ctctgccctg ctatgggata actatacaat
60ctactgtctt tcct
744107DNAHomo sapiens 4gtgactgcat gctcccaggt tgaggtagta ggttgtatag
tttagaatta cacaagggag 60ataactgtac agcctcctag ctttccttgg gtcttgcact
aaacaac 107585DNAHomo sapiens 5ggcggggtga ggtagtaggt
tgtgtggttt cagggcagtg atgttgcccc tcggaagata 60actatacaac ctactgcctt
ccctg 85684DNAHomo sapiens
6gcatccgggt tgaggtagta ggttgtatgg tttagagtta caccctggga gttaactgta
60caaccttcta gctttccttg gagc
84787DNAHomo sapiens 7cctaggaaga ggtagtaggt tgcatagttt tagggcaggg
attttgccca caaggaggta 60actatacgac ctgctgcctt tcttagg
87885DNAHomo sapiens 8ctaggaagag gtagtagttt
gcatagtttt agggcaaaga ttttgcccac aagtagttag 60ctatacgacc tgcagccttt
tgtag 85985DNAHomo sapiens
9ctggctgagg tagtagtttg tgctgttggt cgggttgtga cattgcccgc tgtggagata
60actgcgcaag ctactgcctt gctag
851079DNAHomo sapiens 10cccgggctga ggtaggaggt tgtatagttg aggaggacac
ccaaggagat cactatacgg 60cctcctagct ttccccagg
791187DNAHomo sapiens 11tcagagtgag gtagtagatt
gtatagttgt ggggtagtga ttttaccctg ttcaggagat 60aactatacaa tctattgcct
tccctga 871289DNAHomo sapiens
12ctgtgggatg aggtagtaga ttgtatagtt gtggggtagt gattttaccc tgttcaggag
60ataactatac aatctattgc cttccctga
891385DNAHomo sapiens 13ctgtgggatg aggtagtaga ttgtatagtt ttagggtcat
accccatctt ggagataact 60atacagtcta ctgtctttcc cacgg
8514108DNAHomo sapiens 14ttgcctgatt ccaggctgag
gtagtagttt gtacagtttg agggtctatg ataccacccg 60gtacaggaga taactgtaca
ggccactgcc ttgccaggaa cagcgcgc 1081585DNAHomo sapiens
15ctggctgagg tagtagtttg tgctgttggt cgggttgtga cattgcccgc tgtggagata
60actgcgcaag ctactgcctt gctag
851685DNAHomo sapiens 16acctactcag agtacatact tctttatgta cccatatgaa
catacaatgc tatggaatgt 60aaagaagtat gtatttttgg taggc
8517108DNAHomo sapiens 17cagctaacaa cttagtaata
cctactcaga gtacatactt ctttatgtac ccatatgaac 60atacaatgct atggaatgta
aagaagtatg tatttttggt aggcaata 1081885DNAHomo sapiens
18gcctgcttgg gaaacatact tctttatatg cccatatgga cctgctaagc tatggaatgt
60aaagaagtat gtatctcagg ccggg
851971DNAHomo sapiens 19tgggaaacat acttctttat atgcccatat ggacctgcta
agctatggaa tgtaaagaag 60tatgtatctc a
712085DNAHomo sapiens 20acctactcag agtacatact
tctttatgta cccatatgaa catacaatgc tatggaatgt 60aaagaagtat gtatttttgg
taggc 8521108DNAHomo sapiens
21tggatgttgg cctagttctg tgtggaagac tagtgatttt gttgttttta gataactaaa
60tcgacaacaa atcacagtct gccatatggc acaggccatg cctctaca
10822110DNAHomo sapiens 22ttggatgttg gcctagttct gtgtggaaga ctagtgattt
tgttgttttt agataactaa 60atcgacaaca aatcacagtc tgccatatgg cacaggccat
gcctctacag 11023110DNAHomo sapiens 23ctggatacag agtggaccgg
ctggccccat ctggaagact agtgattttg ttgttgtctt 60actgcgctca acaacaaatc
ccagtctacc taatggtgcc agccatcgca 11024110DNAHomo sapiens
24ctggatacag agtggaccgg ctggccccat ctggaagact agtgattttg ttgttgtctt
60actgcgctca acaacaaatc ccagtctacc taatggtgcc agccatcgca
11025110DNAHomo sapiens 25agattagagt ggctgtggtc tagtgctgtg tggaagacta
gtgattttgt tgttctgatg 60tactacgaca acaagtcaca gccggcctca tagcgcagac
tcccttcgac 11026110DNAHomo sapiens 26agattagagt ggctgtggtc
tagtgctgtg tggaagacta gtgattttgt tgttctgatg 60tactacgaca acaagtcaca
gccggcctca tagcgcagac tcccttcgac 1102789DNAHomo sapiens
27cggggttggt tgttatcttt ggttatctag ctgtatgagt ggtgtggagt cttcataaag
60ctagataacc gaaagtaaaa ataacccca
892887DNAHomo sapiens 28ggaagcgagt tgttatcttt ggttatctag ctgtatgagt
gtattggtct tcataaagct 60agataaccga aagtaaaaac tccttca
872990DNAHomo sapiens 29ggaggcccgt ttctctcttt
ggttatctag ctgtatgagt gccacagagc cgtcataaag 60ctagataacc gaaagtagaa
atgattctca 9030110DNAHomo sapiens
30gatctgtctg tcttctgtat ataccctgta gatccgaatt tgtgtaagga attttgtggt
60cacaaattcg tatctagggg aatatgtagt tgacataaac actccgctct
11031110DNAHomo sapiens 31ccagaggttg taacgttgtc tatatatacc ctgtagaacc
gaatttgtgt ggtatccgta 60tagtcacaga ttcgattcta ggggaatata tggtcgatgc
aaaaacttca 11032108DNAHomo sapiens 32gcgcgaatgt gtgtttaaaa
aaaataaaac cttggagtaa agtagcagca cataatggtt 60tgtggatttt gaaaaggtgc
aggccatatt gtgctgcctc aaaaatac 1083383DNAHomo sapiens
33ccttggagta aagtagcagc acataatggt ttgtggattt tgaaaaggtg caggccatat
60tgtgctgcct caaaaataca agg
833464DNAHomo sapiens 34ctgtagcagc acatcatggt ttacatgcta cagtcaagat
gcgaatcatt atttgctgct 60ctag
643598DNAHomo sapiens 35ttgaggcctt aaagtactgt
agcagcacat catggtttac atgctacagt caagatgcga 60atcattattt gctgctctag
aaatttaagg aaattcat 983689DNAHomo sapiens
36gtcagcagtg ccttagcagc acgtaaatat tggcgttaag attctaaaat tatctccagt
60attaactgtg ctgctgaagt aaggttgac
893781DNAHomo sapiens 37gttccactct agcagcacgt aaatattggc gtagtgaaat
atatattaaa caccaatatt 60actgtgctgc tttagtgtga c
813881DNAHomo sapiens 38gcagtgcctt agcagcacgt
aaatattggc gttaagattc taaaattatc tccagtatta 60actgtgctgc tgaagtaagg t
813984DNAHomo sapiens
39gtcagaataa tgtcaaagtg cttacagtgc aggtagtgat atgtgcatct actgcagtga
60aggcacttgt agcattatgg tgac
844071DNAHomo sapiens 40tgttctaagg tgcatctagt gcagatagtg aagtagatta
gcatctactg ccctaagtgc 60tccttctggc a
714181DNAHomo sapiens 41tttttgttct aaggtgcatc
tagtgcagat agtgaagtag attagcatct actgccctaa 60gtgctccttc tggcataaga a
814282DNAHomo sapiens
42gcagtcctct gttagttttg catagttgca ctacaagaag aatgtagttg tgcaaatcta
60tgcaaaactg atggtggcct gc
824380DNAHomo sapiens 43cagtcctctg ttagttttgc atagttgcac tacaagaaga
atgtagttgt gcaaatctat 60gcaaaactga tggtggcctg
804487DNAHomo sapiens 44cactgttcta tggttagttt
tgcaggtttg catccagctg tgtgatattc tgctgtgcaa 60atccatgcaa aactgactgt
ggtagtg 874596DNAHomo sapiens
45acattgctac ttacaattag ttttgcaggt ttgcatttca gcgtatatat gtatatgtgg
60ctgtgcaaat ccatgcaaaa ctgattgtga taatgt
964680DNAHomo sapiens 46ttctatggtt agttttgcag gtttgcatcc agctgtgtga
tattctgctg tgcaaatcca 60tgcaaaactg actgtggtag
804781DNAHomo sapiens 47ttacaattag ttttgcaggt
ttgcatttca gcgtatatat gtatatgtgg ctgtgcaaat 60ccatgcaaaa ctgattgtga t
814871DNAHomo sapiens
48gtagcactaa agtgcttata gtgcaggtag tgtttagtta tctactgcat tatgagcact
60taaagtactg c
714972DNAHomo sapiens 49tgtcgggtag cttatcagac tgatgttgac tgttgaatct
catggcaaca ccagtcgatg 60ggctgtctga ca
725081DNAHomo sapiens 50accttgtcgg gtagcttatc
agactgatgt tgactgttga atctcatggc aacaccagtc 60gatgggctgt ctgacatttt g
815185DNAHomo sapiens
51ggctgagccg cagtagttct tcagtggcaa gctttatgtc ctgacccagc taaagctgcc
60agttgaagaa ctgttgccct ctgcc
855273DNAHomo sapiens 52ggccggctgg ggttcctggg gatgggattt gcttcctgtc
acaaatcaca ttgccaggga 60tttccaaccg acc
735397DNAHomo sapiens 53ctcaggtgct ctggctgctt
gggttcctgg catgctgatt tgtgacttaa gattaaaatc 60acattgccag ggattaccac
gcaaccacga ccttggc 975481DNAHomo sapiens
54ccacggccgg ctggggttcc tggggatggg atttgcttcc tgtcacaaat cacattgcca
60gggatttcca accgaccctg a
815568DNAHomo sapiens 55ctccggtgcc tactgagctg atatcagttc tcattttaca
cactggctca gttcagcagg 60aacaggag
685673DNAHomo sapiens 56ctctgcctcc cgtgcctact
gagctgaaac acagttggtt tgtgtacact ggctcagttc 60agcaggaaca ggg
735781DNAHomo sapiens
57ccctgggctc tgcctcccgt gcctactgag ctgaaacaca gttggtttgt gtacactggc
60tcagttcagc aggaacaggg g
815871DNAHomo sapiens 58ccctccggtg cctactgagc tgatatcagt tctcatttta
cacactggct cagttcagca 60ggaacagcat c
715984DNAHomo sapiens 59ggccagtgtt gagaggcgga
gacttgggca attgctggac gctgccctgg gcattgcact 60tgtctcggtc tgacagtgcc
ggcc 846086DNAHomo sapiens
60aggccgtggc ctcgttcaag taatccagga taggctgtgc aggtcccaat ggcctatctt
60ggttacttgc acggggacgc gggcct
866177DNAHomo sapiens 61ccgggaccca gttcaagtaa ttcaggatag gttgtgtgct
gtccagcctg ttctccatta 60cttggctcgg ggaccgg
776278DNAHomo sapiens 62ctgaggagca gggcttagct
gcttgtgagc agggtccaca ccaagtcgtg ttcacagtgg 60ctaagttccg ccccccag
786373DNAHomo sapiens
63aggtgcagag cttagctgat tggtgaacag tgattggttt ccgctttgtt cacagtggct
60aagttctgca cct
736497DNAHomo sapiens 64acctctctaa caaggtgcag agcttagctg attggtgaac
agtgattggt ttccgctttg 60ttcacagtgg ctaagttctg cacctgaaga gaaggtg
976580DNAHomo sapiens 65cctgaggagc agggcttagc
tgcttgtgag cagggtccac accaagtcgt gttcacagtg 60gctaagttcc gccccccagg
806686DNAHomo sapiens
66ggtccttgcc ctcaaggagc tcacagtcta ttgagttacc tttctgactt tcccactaga
60ttgtgagctc ctggagggca ggcact
8667108DNAHomo sapiens 67ccttctgtga ccccttagag gatgactgat ttcttttggt
gttcagagtc aatataattt 60tctagcacca tctgaaatcg gttataatga ttggggaaga
gcaccatg 1086864DNAHomo sapiens 68atgactgatt tcttttggtg
ttcagagtca atataatttt ctagcaccat ctgaaatcgg 60ttat
6469110DNAHomo sapiens
69accactggcc catctcttac acaggctgac cgatttctcc tggtgttcag agtctgtttt
60tgtctagcac catttgaaat cggttatgat gtagggggaa aagcagcagc
1107071DNAHomo sapiens 70gcgactgtaa acatcctcga ctggaagctg tgaagccaca
gatgggcttt cagtcggatg 60tttgcagctg c
717160DNAHomo sapiens 71atgtaaacat cctacactca
gctgtaatac atggattggc tgggaggtgg atgtttacgt 607288DNAHomo sapiens
72accaagtttc agttcatgta aacatcctac actcagctgt aatacatgga ttggctggga
60ggtggatgtt tacttcagct gacttgga
887372DNAHomo sapiens 73agatactgta aacatcctac actctcagct gtggaaagta
agaaagctgg gagaaggctg 60tttactcttt ct
727470DNAHomo sapiens 74gttgttgtaa acatccccga
ctggaagctg taagacacag ctaagctttc agtcagatgt 60ttgctgctac
707571DNAHomo sapiens
75ggagaggagg caagatgctg gcatagctgt tgaactggga acctgctatg ccaacatatt
60gccatctttc c
717670DNAHomo sapiens 76ggagatattg cacattacta agttgcatgt tgtcacggcc
tcaatgcaat ttagtgtgtg 60tgatattttc
7077110DNAHomo sapiens 77gggggccgag agaggcgggc
ggccccgcgg tgcattgctg ttgcattgca cgtgtgtgag 60gcgggtgcag tgcctcggca
gtgcagcccg gagccggccc ctggcaccac 1107869DNAHomo sapiens
78ctgtggtgca ttgtagttgc attgcatgtt ctggtggtac ccatgcaatg tttccacagt
60gcatcacag
6979110DNAHomo sapiens 79ggccagctgt gagtgtttct ttggcagtgt cttagctggt
tgttgtgagc aatagtaagg 60aagcaatcag caagtatact gccctagaag tgctgcacgt
tgtggggccc 1108082DNAHomo sapiens 80tcagaataat gtcaaagtgc
ttacagtgca ggtagtgata tgtgcatcta ctgcagtgaa 60ggcacttgta gcattatggt
ga 828178DNAHomo sapiens
81ctttctacac aggttgggat cggttgcaat gctgtgtttc tgtatggtat tgcacttgtc
60ccggcctgtt gagtttgg
788275DNAHomo sapiens 82tcatccctgg gtggggattt gttgcattac ttgtgttcta
tataaagtat tgcacttgtc 60ccggcctgtg gaaga
758380DNAHomo sapiens 83ctgggggctc caaagtgctg
ttcgtgcagg tagtgtgatt acccaaccta ctgctgagct 60agcacttccc gagcccccgg
808480DNAHomo sapiens
84ctgggggctc caaagtgctg ttcgtgcagg tagtgtgatt acccaaccta ctgctgagct
60agcacttccc gagcccccgg
808581DNAHomo sapiens 85aacacagtgg gcactcaata aatgtctgtt gaattgaaat
gcgttacatt caacgggtat 60ttattgagca cccactctgt g
818678DNAHomo sapiens 86tggccgattt tggcactagc
acatttttgc ttgtgtctct ccgctctgag caatcatgtg 60cagtgccaat atgggaaa
788780DNAHomo sapiens
87gtgaggtagt aagttgtatt gttgtggggt agggatatta ggccccaatt agaagataac
60tatacaactt actactttcc
808870DNAHomo sapiens 88ggcacccacc cgtagaaccg accttgcggg gccttcgccg
cacacaagct cgtgtctgtg 60ggtccgtgtc
708981DNAHomo sapiens 89cccattggca taaacccgta
gatccgatct tgtggtgaag tggaccgcac aagctcgctt 60ctatgggtct gtgtcagtgt g
8190108DNAHomo sapiens
90aagagagaag atattgaggc ctgttgccac aaacccgtag atccgaactt gtggtattag
60tccgcacaag cttgtatcta taggtatgtg tctgttaggc aatctcac
1089180DNAHomo sapiens 91cctgttgcca caaacccgta gatccgaact tgtggtatta
gtccgcacaa gcttgtatct 60ataggtatgt gtctgttagg
8092110DNAHomo sapiens 92aggctgccct ggctcagtta
tcacagtgct gatgctgtct attctaaagg tacagtactg 60tgataactga aggatggcag
ccatcttacc ttccatcaga ggagcctcac 1109357DNAHomo sapiens
93tcagttatca cagtgctgat gctgtccatt ctaaaggtac agtactgtga taactga
579475DNAHomo sapiens 94tgccctggct cagttatcac agtgctgatg ctgtctattc
taaaggtaca gtactgtgat 60aactgaagga tggca
759575DNAHomo sapiens 95tgtccttttt cggttatcat
ggtaccgatg ctgtatatct gaaaggtaca gtactgtgat 60aactgaagaa tggtg
759681DNAHomo sapiens
96cttctggaag ctggtttcac atggtggctt agatttttcc atctttgtat ctagcaccat
60ttgaaatcag tgttttagga g
819781DNAHomo sapiens 97cttcaggaag ctggtttcat atggtggttt agatttaaat
agtgattgtc tagcaccatt 60tgaaatcagt gttcttgggg g
819881DNAHomo sapiens 98cttcaggaag ctggtttcat
atggtggttt agatttaaat agtgattgtc tagcaccatt 60tgaaatcagt gttcttgggg g
819978DNAHomo sapiens
99ttgtgctttc agcttcttta cagtgctgcc ttgtagcatt caggtcaagc aacattgtac
60agggctatga aagaacca
7810078DNAHomo sapiens 100ttgtgctttc agcttcttta cagtgctgcc ttgtagcatt
caggtcaagc aacattgtac 60agggctatga aagaacca
7810178DNAHomo sapiens 101tactgccctc ggcttcttta
cagtgctgcc ttgttgcata tggatcaagc agcattgtac 60agggctatga aggcattg
7810278DNAHomo sapiens
102aaatgtcaga cagcccatcg actggtgttg ccatgagatt caacagtcaa catcagtctg
60ataagctacc cgacaagg
7810381DNAHomo sapiens 103tgtgcatcgt ggtcaaatgc tcagactcct gtggtggctg
ctcatgcacc acggatgttt 60gagcatgtgc tacggtgtct a
8110481DNAHomo sapiens 104tgtgcatcgt ggtcaaatgc
tcagactcct gtggtggctg ctcatgcacc acggatgttt 60gagcatgtgc tacggtgtct a
8110581DNAHomo sapiens
105ccttggccat gtaaaagtgc ttacagtgca ggtagctttt tgagatctac tgcaatgtaa
60gcacttctta cattaccatg g
8110681DNAHomo sapiens 106ctctctgctt tcagcttctt tacagtgttg ccttgtggca
tggagttcaa gcagcattgt 60acagggctat caaagcacag a
8110785DNAHomo sapiens 107ccttagcaga gctgtggagt
gtgacaatgg tgtttgtgtc taaactatca aacgccatta 60tcacactaaa tagctactgc
taggc 8510866DNAHomo sapiens
108agctgtggag tgtgacaatg gtgtttgtgt ccaaactatc aaacgccatt atcacactaa
60atagct
6610961DNAHomo sapiens 109acattattac ttttggtacg cgctgtgaca cttcaaactc
gtaccgtgag taataatgcg 60c
61110100DNAHomo sapiens 110tccttcctca ggagaaaggc
ctctctctcc gtgttcacag cggaccttga tttaaatgtc 60catacaatta aggcacgcgg
tgaatgccaa gaatggggct 10011185DNAHomo sapiens
111aggcctctct ctccgtgttc acagcggacc ttgatttaaa tgtccataca attaaggcac
60gcggtgaatg ccaagaatgg ggctg
85112110DNAHomo sapiens 112atcaagatta gaggctctgc tctccgtgtt cacagcggac
cttgatttaa tgtcatacaa 60ttaaggcacg cggtgaatgc caagagcgga gcctacggct
gcacttgaag 110113110DNAHomo sapiens 113cccgccccag
ccctgagggc ccctctgcgt gttcacagcg gaccttgatt taatgtctat 60acaattaagg
cacgcggtga atgccaagag aggcgcctcc gccgctcctt 11011487DNAHomo
sapiens 114tgagggcccc tctgcgtgtt cacagcggac cttgatttaa tgtctataca
attaaggcac 60gcggtgaatg ccaagagagg cgcctcc
8711568DNAHomo sapiens 115ctctgcgtgt tcacagcgga ccttgattta
atgtctatac aattaaggca cgcggtgaat 60gccaagag
6811667DNAHomo sapiens 116ctctccgtgt
tcacagcgga ccttgattta atgtcataca attaaggcac gcggtgaatg 60ccaagag
6711786DNAHomo
sapiens 117tgccagtctc taggtccctg agacccttta acctgtgagg acatccaggg
tcacaggtga 60ggttcttggg agcctggcgt ctggcc
8611865DNAHomo sapiens 118ggtccctgag accctttaac ctgtgaggac
atccagggtc acaggtgagg ttcttgggag 60cctgg
65119108DNAHomo sapiens 119acattgttgc
gctcctctca gtccctgaga ccctaacttg tgatgtttac cgtttaaatc 60cacgggttag
gctcttggga gctgcgagtc gtgcttttgc atcctgga 10812088DNAHomo
sapiens 120tgcgctcctc tcagtccctg agaccctaac ttgtgatgtt taccgtttaa
atccacgggt 60taggctcttg ggagctgcga gtcgtgct
8812189DNAHomo sapiens 121accagacttt tcctagtccc tgagacccta
acttgtgagg tattttagta acatcacaag 60tcaggctctt gggacctagg cggagggga
8912270DNAHomo sapiens 122cctagtccct
gagaccctaa cttgtgaggt attttagtaa catcacaagt caggctcttg 60ggacctaggc
7012385DNAHomo
sapiens 123cgctggcgac gggacattat tacttttggt acgcgctgtg acacttcaaa
ctcgtaccgt 60gagtaataat gcgccgtcca cggca
8512461DNAHomo sapiens 124acattattac ttttggtacg cgctgtgaca
cttcaaactc gtaccgtgag taataatgcg 60c
6112597DNAHomo sapiens 125tgtgatcact
gtctccagcc tgctgaagct cagagggctc tgattcagaa agatcatcgg 60atccgtctga
gcttggctgg tcggaagtct catcatc 9712670DNAHomo
sapiens 126ccagcctgct gaagctcaga gggctctgat tcagaaagat catcggatcc
gtctgagctt 60ggctggtcgg
7012782DNAHomo sapiens 127tgagctgttg gattcggggc cgtagcactg
tctgagaggt ttacatttct cacagtgaac 60cggtctcttt ttcagctgct tc
82128110DNAHomo sapiens 128gcccggcagc
cactgtgcag tgggaagggg ggccgataca ctgtacgaga gtgagtagca 60ggtctcacag
tgaaccggtc tctttcccta ctgtgtcaca ctcctaatgg 11012970DNAHomo
sapiens 129gttggattcg gggccgtagc actgtctgag aggtttacat ttctcacagt
gaaccggtct 60ctttttcagc
7013074DNAHomo sapiens 130tggatctttt tgcggtctgg gcttgctgtt
cctctcaaca gtagtcagga agcccttacc 60ccaaaaagta tcta
7413189DNAHomo sapiens 131tgctgctggc
cagagctctt ttcacattgt gctactgtct gcacctgtca ctagcagtgc 60aatgttaaaa
gggcattggc cgtgtagtg
89132110DNAHomo sapiens 132gccaggaggc ggggttggtt gttatctttg gttatctagc
tgtatgagtg gtgtggagtc 60ttcataaagc tagataaccg aaagtaaaaa taaccccata
cactgcgcag 110133110DNAHomo sapiens 133cacggcgcgg
cagcggcact ggctaaggga ggcccgtttc tctctttggt tatctagctg 60tatgagtgcc
acagagccgt cataaagcta gataaccgaa agtagaaatg 11013472DNAHomo
sapiens 134gttgttatct ttggttatct agctgtatga gtgtattggt cttcataaag
ctagataacc 60gaaagtaaaa ac
72135101DNAHomo sapiens 135ccgcccccgc gtctccaggg caaccgtggc
tttcgattgt tactgtggga actggaggta 60acagtctaca gccatggtcg ccccgcagca
cgcccacgcg c 10113666DNAHomo sapiens 136gggcaaccgt
ggctttcgat tgttactgtg ggaactggag gtaacagtct acagccatgg 60tcgccc
6613788DNAHomo
sapiens 137acaatgcttt gctagagctg gtaaaatgga accaaatcgc ctcttcaatg
gatttggtcc 60ccttcaacca gctgtagcta tgcattga
88138102DNAHomo sapiens 138gggagccaaa tgctttgcta gagctggtaa
aatggaacca aatcgactgt ccaatggatt 60tggtcccctt caaccagctg tagctgtgca
ttgatggcgc cg 10213968DNAHomo sapiens 139gctagagctg
gtaaaatgga accaaatcgc ctcttcaatg gatttggtcc ccttcaacca 60gctgtagc
6814073DNAHomo
sapiens 140cagggtgtgt gactggttga ccagaggggc atgcactgtg ttcaccctgt
gggccaccta 60gtcaccaacc ctc
7314171DNAHomo sapiens 141agggtgtgtg actggttgac cagaggggca
tgcactgtgt tcaccctgtg ggccacctag 60tcaccaaccc t
7114290DNAHomo sapiens 142aggcctcgct
gttctctatg gctttttatt cctatgtgat tctactgctc actcatatag 60ggattggagc
cgtggcgcac ggcggggaca
90143100DNAHomo sapiens 143agataaattc actctagtgc tttatggctt tttattccta
tgtgatagta ataaagtctc 60atgtagggat ggaagccatg aaatacattg tgaaaaatca
10014460DNAHomo sapiens 144ctatggcttt ttattcctat
gtgattctac tgctcactca tatagggatt ggagccgtgg 6014582DNAHomo sapiens
145tgagccctcg gaggactcca tttgttttga tgatggattc ttatgctcca tcatcgtctc
60aaatgagtct tcagagggtt ct
8214662DNAHomo sapiens 146gaggactcca tttgttttga tgatggattc ttatgctcca
tcatcgtctc aaatgagtct 60tc
6214773DNAHomo sapiens 147cttcggtgac gggtattctt
gggtggataa tacggattac gttgttattg cttaagaata 60cgcgtagtcg agg
7314899DNAHomo sapiens
148ccctggcatg gtgtggtggg gcagctggtg ttgtgaatca ggccgttgcc aatcagagaa
60cggctacttc acaacaccag ggccacacca cactacagg
9914984DNAHomo sapiens 149cgttgctgca gctggtgttg tgaatcaggc cgacgagcag
cgcatcctct tacccggcta 60tttcacgaca ccagggttgc atca
8415071DNAHomo sapiens 150cagctggtgt tgtgaatcag
gccgacgagc agcgcatcct cttacccggc tatttcacga 60caccagggtt g
7115168DNAHomo sapiens
151gtgtattcta cagtgcacgt gtctccagtg tggctcggag gctggagacg cggccctgtt
60ggagtaac
68152100DNAHomo sapiens 152tgtgtctctc tctgtgtcct gccagtggtt ttaccctatg
gtaggttacg tcatgctgtt 60ctaccacagg gtagaaccac ggacaggata ccggggcacc
10015372DNAHomo sapiens 153tcctgccagt ggttttaccc
tatggtaggt tacgtcatgc tgttctacca cagggtagaa 60ccacggacag ga
7215470DNAHomo sapiens
154cctgccagtg gttttaccct atggtaggtt acgtcatgct gttctaccac agggtagaac
60cacggacagg
7015595DNAHomo sapiens 155cggccggccc tgggtccatc ttccagtaca gtgttggatg
gtctaattgt gaagctccta 60acactgtctg gtaaagatgg ctcccgggtg ggttc
9515672DNAHomo sapiens 156gggtccatct tccagtacag
tgttggatgg tctaattgtg aagctcctaa cactgtctgg 60taaagatggc cc
7215764DNAHomo sapiens
157acccataaag tagaaagcac tactaacagc actggagggt gtagtgtttc ctactttatg
60gatg
6415887DNAHomo sapiens 158gacagtgcag tcacccataa agtagaaagc actactaaca
gcactggagg gtgtagtgtt 60tcctacttta tggatgagtg tactgtg
8715964DNAHomo sapiens 159acccataaag tagaaagcac
tactaacagc actggagggt gtagtgtttc ctactttatg 60gatg
64160106DNAHomo sapiens
160gcgcagcgcc ctgtctccca gcctgaggtg cagtgctgca tctctggtca gttgggagtc
60tgagatgaag cactgtagct caggaagaga gaagttgttc tgcagc
10616163DNAHomo sapiens 161cctgaggtgc agtgctgcat ctctggtcag ttgggagtct
gagatgaagc actgtagctc 60agg
6316286DNAHomo sapiens 162tggggccctg gctgggatat
catcatatac tgtaagtttg cgatgagaca ctacagtata 60gatgatgtac tagtccgggc
accccc 8616366DNAHomo sapiens
163ggctgggata tcatcatata ctgtaagttt gcgatgagac actacagtat agatgatgta
60ctagtc
6616488DNAHomo sapiens 164caccttgtcc tcacggtcca gttttcccag gaatccctta
gatgctaaga tggggattcc 60tggaaatact gttcttgagg tcatggtt
8816570DNAHomo sapiens 165ctcacggtcc agttttccca
ggaatccctt agatgctaag atggggattc ctggaaatac 60tgttcttgag
7016699DNAHomo sapiens
166ccgatgtgta tcctcagctt tgagaactga attccatggg ttgtgtcagt gtcagacctc
60tgaaattcag ttcttcagct gggatatctc tgtcatcgt
9916765DNAHomo sapiens 167agctttgaga actgaattcc atgggttgtg tcagtgtcag
acctgtgaaa ttcagttctt 60cagct
6516872DNAHomo sapiens 168aatctaaaga caacatttct
gcacacacac cagactatgg aagccagtgt gtggaaatgc 60ttctgctaga tt
7216968DNAHomo sapiens
169gaggcaaagt tctgagacac tccgactctg agtatgatag aagtcagtgc actacagaac
60tttgtctc
6817089DNAHomo sapiens 170gccggcgccc gagctctggc tccgtgtctt cactcccgtg
cttgtccgag gagggaggga 60gggacggggg ctgtgctggg gcagctgga
8917153DNAHomo sapiens 171gctctggctc cgtgtcttca
ctcccgtgct tgtccgagga gggagggagg gac 5317284DNAHomo sapiens
172ctccccatgg ccctgtctcc caacccttgt accagtgctg ggctcagacc ctggtacagg
60cctgggggac agggacctgg ggac
8417364DNAHomo sapiens 173ccctgtctcc caacccttgt accagtgctg ggctcagacc
ctggtacagg cctgggggac 60aggg
6417469DNAHomo sapiens 174cctgccctcg aggagctcac
agtctagtat gtctcatccc ctactagact gaagctcctt 60gaggacagg
6917587DNAHomo sapiens
175tgtccccccc ggcccaggtt ctgtgataca ctccgactcg ggctctggag cagtcagtgc
60atgacagaac ttgggcccgg aaggacc
8717671DNAHomo sapiens 176ggcccaggtt ctgtgataca ctccgactcg ggctctggag
cagtcagtgc atgacagaac 60ttgggccccg g
7117790DNAHomo sapiens 177ctcacagctg ccagtgtcat
ttttgtgatc tgcagctagt attctcactc cagttgcata 60gtcacaaaag tgatcattgg
caggtgtggc 9017871DNAHomo sapiens
178tctctctctc cctcacagct gccagtgtca ttgtcacaaa agtgatcatt ggcaggtgtg
60gctgctgcat g
7117987DNAHomo sapiens 179agcggtggcc agtgtcattt ttgtgatgtt gcagctagta
atatgagccc agttgcatag 60tcacaaaagt gatcattgga aactgtg
8718069DNAHomo sapiens 180cagtgtcatt tttgtgatgt
tgcagctagt aatatgagcc cagttgcata gtcacaaaag 60tgatcattg
6918184DNAHomo sapiens
181gtggtacttg aagataggtt atccgtgttg ccttcgcttt atttgtgacg aatcatacac
60ggttgaccta tttttcagta ccaa
8418266DNAHomo sapiens 182gaagataggt tatccgtgtt gccttcgctt tatttgtgac
gaatcataca cggttgacct 60attttt
6618365DNAHomo sapiens 183ctgttaatgc taatcgtgat
aggggttttt gcctccaact gactcctaca tattagcatt 60aacag
65184108DNAHomo sapiens
184caatgtcagc agtgccttag cagcacgtaa atattggcgt taagattcta aaattatctc
60cagtattaac tgtgctgctg aagtaaggtt gaccatactc tacagttg
108185110DNAHomo sapiens 185agaagggcta tcaggccagc cttcagagga ctccaaggaa
cattcaacgc tgtcggtgag 60tttgggattt gaaaaaacca ctgaccgttg actgtacctt
ggggtcctta 110186110DNAHomo sapiens 186tgagttttga
ggttgcttca gtgaacattc aacgctgtcg gtgagtttgg aattaaaatc 60aaaaccatcg
accgttgatt gtaccctatg gctaaccatc atctactcca
110187110DNAHomo sapiens 187cggaaaattt gccaagggtt tgggggaaca ttcaacctgt
cggtgagttt gggcagctca 60ggcaaaccat cgaccgttga gtggaccctg aggcctggaa
ttgccatcct 110188110DNAHomo sapiens 188gagctgcttg
cctccccccg tttttggcaa tggtagaact cacactggtg aggtaacagg 60atccggtggt
tctagacttg ccaactatgg ggcgaggact cagccggcac 11018970DNAHomo
sapiens 189tttttggcaa tggtagaact cacactggtg aggtaacagg atccggtggt
tctagacttg 60ccaactatgg
70190110DNAHomo sapiens 190ccgcagagtg tgactcctgt tctgtgtatg
gcactggtag aattcactgt gaacagtctc 60agtcagtgaa ttaccgaagg gccataaaca
gagcagagac agatccacga 11019184DNAHomo sapiens 191ccagtcacgt
ccccttatca cttttccagc ccagctttgt gactgtaagt gttggacgga 60gaactgataa
gggtaggtga ttga 8419265DNAHomo
sapiens 192ccttatcact tttccagccc agctttgtga ctgtaagtgt tggacggaga
actgataagg 60gtagg
6519382DNAHomo sapiens 193agggggcgag ggattggaga gaaaggcagt
tcctgatggt cccctcccca ggggctggct 60ttcctctggt ccttccctcc ca
8219466DNAHomo sapiens 194agggattgga
gagaaaggca gttcctgatg gtcccctccc caggggctgg ctttcctctg 60gtcctt
6619586DNAHomo
sapiens 195tgcttgtaac tttccaaaga attctccttt tgggctttct ggttttattt
taagcccaaa 60ggtgaatttt ttgggaagtt tgagct
8619671DNAHomo sapiens 196actttccaaa gaattctcct tttgggcttt
ctggttttat tttaagccca aaggtgaatt 60ttttgggaag t
71197109DNAHomo sapiens 197ggtcgggctc
accatgacac agtgtgagac tcgggctaca acacaggacc cggggcgctg 60ctctgacccc
tcgtgtcttg tgttgcagcc ggagggacgc aggtccgca 10919886DNAHomo
sapiens 198tgctccctct ctcacatccc ttgcatggtg gagggtgagc tttctgaaaa
cccctcccac 60atgcagggtt tgcaggatgg cgagcc
8619968DNAHomo sapiens 199tctcacatcc cttgcatggt ggagggtgag
ctttctgaaa acccctccca catgcagggt 60ttgcagga
68200102DNAHomo sapiens 200ctgtcgattg
gacccgccct ccggtgccta ctgagctgat atcagttctc attttacaca 60ctggctcagt
tcagcaggaa caggagtcga gcccttgagc aa 10220168DNAHomo
sapiens 201ctccggtgcc tactgagctg atatcagttc tcattttaca cactggctca
gttcagcagg 60aacaggag
6820285DNAHomo sapiens 202tgcaggcctc tgtgtgatat gtttgatata
ttaggttgtt atttaatcca actatatatc 60aaacatattc ctacagtgtc ttgcc
8520367DNAHomo sapiens 203ctgtgtgata
tgtttgatat attaggttgt tatttaatcc aactatatat caaacatatt 60cctacag
6720492DNAHomo
sapiens 204cggctggaca gcgggcaacg gaatcccaaa agcagctgtt gtctccagag
cattccagct 60gcgcttggat ttcgtcccct gctctcctgc ct
9220574DNAHomo sapiens 205agcgggcaac ggaatcccaa aagcagctgt
tgtctccaga gcattccagc tgcgcttgga 60tttcgtcccc tgct
74206108DNAHomo sapiens 206ccgagaccga
gtgcacaggg ctctgaccta tgaattgaca gccagtgctc tcgtctcccc 60tctggctgcc
aattccatag gtcacaggta tgttcgcctc aatgccag
108207110DNAHomo sapiens 207gccgagaccg agtgcacagg gctctgacct atgaattgac
agccagtgct ctcgtctccc 60ctctggctgc caattccata ggtcacaggt atgttcgcct
caatgccagc 11020888DNAHomo sapiens 208cgaggatggg agctgagggc
tgggtctttg cgggcgagat gagggtgtcg gatcaactgg 60cctacaaagt cccagttctc
ggcccccg 8820958DNAHomo sapiens
209gctgggtctt tgcgggcgag atgagggtgt cggatcaact ggcctacaaa gtcccagt
5821085DNAHomo sapiens 210atggtgttat caagtgtaac agcaactcca tgtggactgt
gtaccaattt ccagtggaga 60tgctgttact tttgatggtt accaa
8521163DNAHomo sapiens 211gtgtaacagc aactccatgt
ggactgtgta ccaatttcca gtggagatgc tgttactttt 60gat
6321287DNAHomo sapiens
212agcttccctg gctctagcag cacagaaata ttggcacagg gaagcgagtc tgccaatatt
60ggctgtgctg ctccaggcag ggtggtg
8721358DNAHomo sapiens 213tagcagcaca gaaatattgg cacagggaag cgagtctgcc
aatattggct gtgctgct 58214110DNAHomo sapiens 214ctagagcttg
aattggaact gctgagtgaa ttaggtagtt tcatgttgtt gggcctgggt 60ttctgaacac
aacaacatta aaccacccga ttcacggcag ttactgctcc 11021570DNAHomo
sapiens 215gtgaattagg tagtttcatg ttgttgggcc tgggtttctg aacacaacaa
cattaaacca 60cccgattcac
70216110DNAHomo sapiens 216tgctcgctca gctgatctgt ggcttaggta
gtttcatgtt gttgggattg agttttgaac 60tcggcaacaa gaaactgcct gagttacatc
agtcggtttt cgtcgagggc 11021770DNAHomo sapiens 217gtgaattagg
tagtttcatg ttgttgggcc tgggtttctg aacacaacaa cattaaacca 60cccgattcac
7021875DNAHomo
sapiens 218ggctgtgccg ggtagagagg gcagtgggag gtaagagctc ttcacccttc
accaccttct 60ccacccagca tggcc
7521962DNAHomo sapiens 219tcattggtcc agaggggaga taggttcctg
tgatttttcc ttcttctcta tagaataaat 60ga
6222071DNAHomo sapiens 220gccaacccag
tgttcagact acctgttcag gaggctctca atgtgtacag tagtctgcac 60attggttagg c
71221110DNAHomo
sapiens 221aggaagcttc tggagatcct gctccgtcgc cccagtgttc agactacctg
ttcaggacaa 60tgccgttgta cagtagtctg cacattggtt agactgggca agggagagca
110222110DNAHomo sapiens 222ccagaggaca cctccactcc gtctacccag
tgtttagact atctgttcag gactcccaaa 60ttgtacagta gtctgcacat tggttaggct
gggctgggtt agaccctcgg 11022371DNAHomo sapiens 223gccaacccag
tgttcagact acctgttcag gaggctctca atgtgtacag tagtctgcac 60attggttagg c
7122470DNAHomo
sapiens 224gccgtggcca tcttactggg cagcattgga tggagtcagg tctctaatac
tgcctggtaa 60tgatgacggc
7022595DNAHomo sapiens 225ccagctcggg cagccgtggc catcttactg
ggcagcattg gatggagtca ggtctctaat 60actgcctggt aatgatgacg gcggagccct
gcacg 9522672DNAHomo sapiens 226gttccttttt
cctatgcata tacttctttg aggatctggc ctaaagaggt atagggcatg 60ggaagatgga
gc
72227110DNAHomo sapiens 227gtgttgggga ctcgcgcgct gggtccagtg gttcttaaca
gttcaacagt tctgtagcgc 60aattgtgaaa tgtttaggac cactagaccc ggcgggcgcg
gcgacagcga 110228110DNAHomo sapiens 228ggctacagtc
tttcttcatg tgactcgtgg acttcccttt gtcatcctat gcctgagaat 60atatgaagga
ggctgggaag gcaaagggac gttcaattgt catcactggc
110229110DNAHomo sapiens 229aaagatcctc agacaatcca tgtgcttctc ttgtccttca
ttccaccgga gtctgtctca 60tacccaacca gatttcagtg gagtgaagtt caggaggcat
ggagctgaca 11023086DNAHomo sapiens 230tgcttcccga ggccacatgc
ttctttatat ccccatatgg attactttgc tatggaatgt 60aaggaagtgt gtggtttcgg
caagtg 8623169DNAHomo sapiens
231aggccacatg cttctttata tccccatatg gattactttg ctatggaatg taaggaagtg
60tgtggtttt
6923271DNAHomo sapiens 232tgacgggcga gcttttggcc cgggttatac ctgatgctca
cgtataagac gagcaaaaag 60cttgttggtc a
71233110DNAHomo sapiens 233acccggcagt gcctccaggc
gcagggcagc ccctgcccac cgcacactgc gctgccccag 60acccactgtg cgtgtgacag
cggctgatct gtgcctgggc agcgcgaccc 110234110DNAHomo sapiens
234tcacctggcc atgtgacttg tgggcttccc tttgtcatcc ttcgcctagg gctctgagca
60gggcagggac agcaaagggg tgctcagttg tcacttccca cagcacggag
110235110DNAHomo sapiens 235cggggcaccc cgcccggaca gcgcgccggc accttggctc
tagactgctt actgcccggg 60ccgccctcag taacagtctc cagtcacggc caccgacgcc
tggccccgcc 110236110DNAHomo sapiens 236cctgtgcaga
gattattttt taaaaggtca caatcaacat tcattgctgt cggtgggttg 60aactgtgtgg
acaagctcac tgaacaatga atgcaactgt ggccccgctt
110237108DNAHomo sapiens 237gagttttgag gttgcttcag tgaacattca acgctgtcgg
tgagtttgga attaaaatca 60aaaccatcga ccgttgattg taccctatgg ctaaccatca
tctactcc 108238110DNAHomo sapiens 238ggcctggctg
gacagagttg tcatgtgtct gcctgtctac acttgctgtg cagaacatcc 60gctcacctgt
acagcaggca cagacaggca gtcacatgac aacccagcct
110239110DNAHomo sapiens 239atcattcaga aatggtatac aggaaaatga cctatgaatt
gacagacaat atagctgagt 60ttgtctgtca tttctttagg ccaatattct gtatgactgt
gctacttcaa 110240110DNAHomo sapiens 240gatggctgtg
agttggctta atctcagctg gcaactgtga gatgttcata caatccctca 60cagtggtctc
tgggattatg ctaaacagag caatttccta gccctcacga
110241110DNAHomo sapiens 241agtataatta ttacatagtt tttgatgtcg cagatactgc
atcaggaact gattggataa 60gaatcagtca ccatcagttc ctaatgcatt gccttcagca
tctaaacaag 110242110DNAHomo sapiens 242gtgataatgt
agcgagattt tctgttgtgc ttgatctaac catgtggttg cgaggtatga 60gtaaaacatg
gttccgtcaa gcaccatgga acgtcacgca gctttctaca
110243110DNAHomo sapiens 243gaccagtcgc tgcggggctt tcctttgtgc ttgatctaac
catgtggtgg aacgatggaa 60acggaacatg gttctgtcaa gcaccgcgga aagcaccgtg
ctctcctgca 110244110DNAHomo sapiens 244ccgccccggg
ccgcggctcc tgattgtcca aacgcaattc tcgagtctat ggctccggcc 60gagagttgag
tctggacgtc ccgagccgcc gcccccaaac ctcgagcggg
110245110DNAHomo sapiens 245gacagtgtgg cattgtaggg ctccacaccg tatctgacac
tttgggcgag ggcaccatgc 60tgaaggtgtt catgatgcgg tctgggaact cctcacggat
cttactgatg 110246110DNAHomo sapiens 246tgaacatcca
ggtctggggc atgaacctgg catacaatgt agatttctgt gttcgttagg 60caacagctac
attgtctgct gggtttcagg ctacctggaa acatgttctc
110247110DNAHomo sapiens 247gctgctggaa ggtgtaggta ccctcaatgg ctcagtagcc
agtgtagatc ctgtctttcg 60taatcagcag ctacatctgg ctactgggtc tctgatggca
tcttctagct 110248110DNAHomo sapiens 248cctggcctcc
tgcagtgcca cgctccgtgt atttgacaag ctgagttgga cactccatgt 60ggtagagtgt
cagtttgtca aataccccaa gtgcggcaca tgcttaccag 11024981DNAHomo
sapiens 249gggctttcaa gtcactagtg gttccgttta gtagatgatt gtgcattgtt
tcaaaatggt 60gccctagtga ctacaaagcc c
8125081DNAHomo sapiens 250cttctggaag ctggtttcac atggtggctt
agatttttcc atctttgtat ctagcaccat 60ttgaaatcag tgttttagga g
8125181DNAHomo sapiens 251cttcaggaag
ctggtttcat atggtggttt agatttaaat agtgattgtc tagcaccatt 60tgaaatcagt
gttcttgggg g 8125281DNAHomo
sapiens 252cttcaggaag ctggtttcat atggtggttt agatttaaat agtgattgtc
tagcaccatt 60tgaaatcagt gttcttgggg g
8125380DNAHomo sapiens 253gtgagcgact gtaaacatcc tcgactggaa
gctgtgaagc cacagatggg ctttcagtcg 60gatgtttgca gctgcctact
8025488DNAHomo sapiens 254accaagtttc
agttcatgta aacatcctac actcagctgt aatacatgga ttggctggga 60ggtggatgtt
tacttcagct gacttgga 8825575DNAHomo
sapiens 255tgccctggct cagttatcac agtgctgatg ctgtctattc taaaggtaca
gtactgtgat 60aactgaagga tggca
7525690DNAHomo sapiens 256acactgcaag aacaataagg atttttaggg
gcattatgac tgagtcagaa aacacagctg 60cccctgaaag tccctcattt ttcttgctgt
9025780DNAHomo sapiens 257actgcaagag
caataaggat ttttaggggc attatgatag tggaatggaa acacatctgc 60ccccaaaagt
ccctcatttt 8025885DNAHomo
sapiens 258cgctggcgac gggacattat tacttttggt acgcgctgtg acacttcaaa
ctcgtaccgt 60gagtaataat gcgccgtcca cggca
8525961DNAHomo sapiens 259acattattac ttttggtacg cgctgtgaca
cttcaaactc gtaccgtgag taataatgcg 60c
6126074DNAHomo sapiens 260tggatctttt
tgcggtctgg gcttgctgtt cctctcaaca gtagtcagga agcccttacc 60ccaaaaagta
tcta 7426190DNAHomo
sapiens 261tgcccttcgc gaatcttttt gcggtctggg cttgctgtac ataactcaat
agccggaagc 60ccttacccca aaaagcattt gcggagggcg
9026280DNAHomo sapiens 262gccccctgct ctggctggtc aaacggaacc
aagtccgtct tcctgagagg tttggtcccc 60ttcaaccagc tacagcaggg
80263100DNAHomo sapiens 263agataaattc
actctagtgc tttatggctt tttattccta tgtgatagta ataaagtctc 60atgtagggat
ggaagccatg aaatacattg tgaaaaatca 10026470DNAHomo
sapiens 264aagcacgatt agcatttgag gtgaagttct gttatacact caggctgtgg
ctctctgaaa 60gtcagtgcat
7026569DNAHomo sapiens 265cctgtcctca aggagcttca gtctagtagg
ggatgagaca tactagactg tgagctcctc 60gagggcagg
6926665DNAHomo sapiens 266ctgttaatgc
taatcgtgat aggggttttt gcctccaact gactcctaca tattagcatt 60aacag
6526782DNAHomo
sapiens 267cctaacactg tctggtaaag atggctcccg ggtgggttct ctcggcagta
accttcaggg 60agccctgaag accatggagg ac
82268110DNAHomo sapiens 268gccgagaccg agtgcacagg gctctgacct
atgaattgac agccagtgct ctcgtctccc 60ctctggctgc caattccata ggtcacaggt
atgttcgcct caatgccagc 11026980DNAHomo sapiens 269tcccgccccc
tgtaacagca actccatgtg gaagtgccca ctggttccag tggggctgct 60gttatctggg
gcgagggcca 8027070DNAHomo
sapiens 270aaagctgggt tgagagggcg aaaaaggatg aggtgactgg tctgggctac
gctatgctgc 60ggcgctcggg
7027164DNAHomo sapiens 271cattggcctc ctaagccagg gattgtgggt
tcgagtccca cccggggtaa agaaaggccg 60aatt
6427270DNAHomo sapiens 272cctaagccag
ggattgtggg ttcgagtccc acctggggta gaggtgaaag ttccttttac 60ggaatttttt
7027381DNAHomo
sapiens 273gggctttcaa gtcactagtg gttccgttta gtagatgatt gtgcattgtt
tcaaaatggt 60gccctagtga ctacaaagcc c
8127470DNAHomo sapiens 274acgcaagtgt cctaaggtga gctcagggag
cacagaaacc tccagtggaa cagaagggca 60aaagctcatt
7027570DNAHomo sapiens 275catgtgtcac
tttcaggtgg agtttcaaga gtcccttcct ggttcaccgt ctcctttgct 60cttccacaac
70276109DNAHomo
sapiens 276ggtcgggctc accatgacac agtgtgagac tcgggctaca acacaggacc
cggggcgctg 60ctctgacccc tcgtgtcttg tgttgcagcc ggagggacgc aggtccgca
10927786DNAHomo sapiens 277tgctccctct ctcacatccc ttgcatggtg
gagggtgagc tttctgaaaa cccctcccac 60atgcagggtt tgcaggatgg cgagcc
8627885DNAHomo sapiens 278tgcaggcctc
tgtgtgatat gtttgatata ttaggttgtt atttaatcca actatatatc 60aaacatattc
ctacagtgtc ttgcc 8527960DNAHomo
sapiens 279gtgcatgtgt atgtatgtgt gcatgtgcat gtgtatgtgt atgagtgcat
gcgtgtgtgc 6028075DNAHomo sapiens 280ggctgtgccg ggtagagagg gcagtgggag
gtaagagctc ttcacccttc accaccttct 60ccacccagca tggcc
7528172DNAHomo sapiens 281gttccttttt
cctatgcata tacttctttg aggatctggc ctaaagaggt atagggcatg 60ggaagatgga
gc 7228260DNAHomo
sapiens 282caatcttcct ttatcatggt attgattttt cagtgcttcc cttttgtgtg
agagaagata 60283102DNAHomo sapiens 283atggagctgc tcaccctgtg
ggcctcaaat gtggaggaac tattctgatg tccaagtgga 60aagtgctgcg acatttgagc
gtcaccggtg acgcccatat ca 102284101DNAHomo sapiens
284gcatcccctc agcctgtggc actcaaactg tgggggcact ttctgctctc tggtgaaagt
60gccgccatct tttgagtgtt accgcttgag aagactcaac c
101285102DNAHomo sapiens 285cgaggagctc atactgggat actcaaaatg ggggcgcttt
cctttttgtc tgttactggg 60aagtgcttcg attttggggt gtccctgttt gagtagggca
tc 10228681DNAHomo sapiens 286cttcaggaag ctggtttcat
atggtggttt agatttaaat agtgattgtc tagcaccatt 60tgaaatcagt gttcttgggg g
8128722DNAArtificial
SequenceDescription of Artificial Sequence Synthetic oligonucleotide
287tcaacatcag tctgataagc ta
2228822DNAArtificial SequenceDescription of Artificial Sequence Synthetic
oligonucleotide 288tcacaagtta gggtctcagg ga
2228924DNAArtificial SequenceDescription of
Artificial Sequence Synthetic oligonucleotide 289aagggattcc
tgggaaaact ggac
2429030DNAArtificial SequenceDescription of Artificial Sequence Synthetic
oligonucleotide 290gcaggggcca tgctaatctt ctctgtatcg
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