Patent application title: DETECTION OF NEGATIVELY CHARGED POLYMERS USING WATER-SOLUBLE, CATIONIC, POLYTHIOPHENE DERIVATIVES
Mario Leclerc (Quebec, CA)
Hoang Anh Ho (Sainte-Foy, CA)
Maurice Boissinot (Saint-Augustin-De-Desmaures, CA)
Maurice Boissinot (Saint-Augustin-De-Desmaures, CA)
IPC8 Class: AC12Q168FI
Class name: Chemistry: molecular biology and microbiology measuring or testing process involving enzymes or micro-organisms; composition or test strip therefore; processes of forming such composition or test strip involving nucleic acid
Publication date: 2010-02-11
Patent application number: 20100035272
Novel methods allowing for the simple optical and electrochemical
detection of double-stranded oligonucleotides are disclosed. The methods
are rapid, selective and versatile. Advantageously, they do not require
any chemical reaction on the probes or on the analytes since they are
based on different electrostatic interactions between cationic
poly(3-alkoxy-4-methylthiophene) derivatives and single-stranded or
double-stranded (hybridized) oligonucleotides.
1. A process for polymerizing a thiophene monomer, wherein said thiophene
monomer comprises a compound having the formula: ##STR00009## wherein:a)
m is an integer ranging from 2 to 3;b) R* is a quaternary ammonium;c) Y
is an oxygen atom or a methylene; andd) R1 is a methyl group or a
hydrogen atom; wherein said process comprises:reacting said monomer in
the presence of an oxidizing agent, resulting in the formation of a
polymer of the following general formula: ##STR00010## wherein:a) m is an
integer ranging for 2 to 3;b) n is an integer ranging from 3 to 100;c) R*
is a quaternary ammonium;d) Y is an oxygen atom or a methylene; ande)
R1 is a methyl group or a hydrogen atom.
2. The process of claim 1, wherein said oxidizing agent is FeCl3 or K2S2O.sub.8.
3. The process of claim 1, wherein when R1 is a methyl group, said polymer is regio-regular and water soluble.
4. The process of claim 3, wherein said polymer is thermostable.
5. A polymer obtained from the process of claim 1.
6. A method for discriminating a first nucleic acid from a second nucleic acid, said second nucleic acid differing from said first nucleic acid by at least one nucleotide, comprising the steps of:a) contacting a complementary target to said first nucleic acid with a polymer of the following general formula ##STR00011## wherein:a) m is an integer ranging for 2 to 3;b) n is an integer ranging from 3 to 100;c) R* is a quaternary ammonium;d) Y is an oxygen atom or a methylene; ande) R1 is a methyl group or a hydrogen atom, to form a duplex;b) contacting said duplex with said first nucleic acid, resulting in specific hybridization;c) contacting said duplex with said second nucleic acid, resulting in non-specific hybridization; andd) detecting a change in electronic charge, fluorescence or color.
7. The method of claim 6, wherein said change in electronic charge, fluorescence or color is more pronounced with said specific hybridization.
8. The method of claim 7, wherein said complementary target is a nucleic acid complementary to said first nucleic acid.
9. The method of claim 8, wherein said first nucleic acid and said second nucleic acid are DNA.
10. The method of claim 8, wherein said first nucleic acid and said second nucleic acid are RNA.
11. The method of claim 8, wherein said complementary target is a nucleic acid probe and said first nucleic acid and said second nucleic acid are denatured PCR amplicons.
The present application is a divisional of U.S. Ser. No. 12/247,058, filed on Oct. 7, 2008, and entitled "DETECTION OF NEGATIVELY CHARGED POLYMERS USING WATER-SOLUBLE, CATIONIC, POLYTHIOPHENE DERIVATIVES," which is a divisional of U.S. Ser. No. 11/370,158, filed on Mar. 7, 2006, by Leclerc et al., and entitled "DETECTION OF NEGATIVELY CHARGED POLYMERS USING WATER-SOLUBLE, CATIONIC, POLYTHIOPHENE DERIVATIVES," issued as U.S. Pat. No. 7,446,213, which is a divisional U.S. Ser. No. 10/474,230, filed on Apr. 5, 2004, by Leclerc, et al., and entitled "DETECTION OF NEGATIVELY CHARGED POLYMERS USING WATER-SOLUBLE, CATIONIC, POLYTHIOPHENE DERIVATIVES," issued as U.S. Pat. No. 7,083,928, which claims priority under 35 U.S.C. §119(a) to PCT/CA02/00485 filed Apr. 5, 2002, entitled "DETECTION OF NEGATIVELY CHARGED POLYMERS USING WATER-SOLUBLE, CATIONIC, POLYTHIOPHENE DERIVATIVES," which claims priority to U.S. Provisional Application No. 60/281,371, filed Apr. 5, 2001; U.S. Provisional Application No. 60/284,184 filed April 18, 2001; and U.S. Provisional Application No. 60/288,442 filed May 4, 2001, each of which is hereby expressly incorporated in its entirety.
REFERENCE TO SEQUENCE LISTING
The present application is being filed along with a sequence listing in electronic format. The sequence listing is provided as a file entitled GENOM.052NPDDD.txt, created Oct. 9, 2009 which is 3.29 KB in size. The information in the electronic format of the sequence listing is incorporated herein by reference in its entirety.
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to simple and reliable methods for negatively charged polymer detection, namely for sequence-selective nucleic acid detection. More specifically, the present invention relates to sequence-selective nucleic acid detection methods, which are essential for the rapid diagnosis of infections and a variety of diseases.
2. Description of the Related Art
Complexes of polythiophene derivatives bearing sulfonic acid moieties and one or several adequately designed amine-containing molecules (electrostatic interactions) have been shown to be responsive to external stimuli (PCT/CA98/01082). More specifically, they were shown to undergo striking conformational changes when exposed to heat, light or various chemical and biochemical moieties giving rise to thermochromism, photochromism, ionochromism or even biochromism. These sulfonic acid-bearing polythiophene derivatives are not positively charged and thus do not have any particular affinity for negatively charged polymers.
The search for methods for sequence-selective nucleic acid detection has evolved into an important research field and has subsequently drawn the attention of researchers from various disciplines such as chemistry, physics, biochemistry, etc. As a result, some interesting DNA hybridization sensors have recently been proposed. (Fodor, S. P. A et al. Science 251, 767, (1991); Livache, T. et al. Nucleic Acids Res. 22, 2912 (1994); Tyagi, S.; Kramer, F. R. Nature Biotechnology 14, 303 (1996); Mikkelson, S. R. Electroanalysis 8, 15 (1996); Taton, T. A.; Mirkin, C. A.; Letsinger, R. L. Science 289, 1757, (2000)).
However, most of these newly developed approaches perform detection by attaching a fluorescent or electro-active tag to the analyte.
Assays that do not require nucleic acid functionalization prior to detection are of greater fidelity and several research groups have reported the utilization of conjugated field-responsive polymers (polypyrroles, polythiophenes, etc.) as electrochemical or optical transducers. (Leclerc, M. Adv. Mater. 11, 1491, (1999); McQuade, D. T.; Pullen, A. E.; Swager, T. M. Chem. Rev. 100, 2537 (2000); Chen, L., McBranch, D. W., Wang, H. L., Hegelson, R., Wudl, F. & Whitten, D. G. Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer. PNAS 96, 12287 (1999); Ewbank, P. C., Nuding, G., Suenaga, H., McCullough, R. D., Shinkai, S. Amine functionalized polythiophenes: synthesis and formation of chiral, ordered structures on DNA substrates. Tetrahedron Lett. 42, 155 (2001)). Indeed, the ability of some oligonucleotide-functionalized conjugated polymers to transduce hybridization events into an electrical or optical signal, without utilizing any labeling of the analyte, has been demonstrated. (Youssoufi, H. K.; Garnier, F.; Srivastava, P.; Godillot, P.; Yassar, A. J. Am. Chem. Soc., 119, 7388, (1997); Bauerle, P.; Emge, A. Adv. Matter. 10, 324, (1998); Garnier, F.; Youssoufi, H. K.; Srivastava, P.; Mandrand, B.; Delair, T. Synth. Metals, 100, 89, (1999)). The detection mechanism is based on a modification of the electrical and/or optical properties through the capture of the complementary oligonucleotides.
There thus remains a need for simpler, more sensitive and more reliable methods for the rapid and specific identification of nucleic acids. These nucleic acids could be used for the diagnosis of infections and disease. Ideally, an assay that does not require nucleic acid functionalization (chemical manipulation of nucleic acids) prior to detection nor complex reaction mixtures would have the following characteristics: it would be simpler to use than the assays currently available and it would have a high degree of fidelity. Such an assay would be highly beneficial and therefore very desirable.
The present invention seeks to meet these and other needs.
SUMMARY OF THE INVENTION
In general terms, the present invention relates to novel cationic, water-soluble polythiophene derivatives, which can readily transduce oligonucleotide hybridization into a clearly interpretable optical (calorimetric, fluorescent or luminescent) or electrical signal. These polymers can discriminate between specific and non-specific hybridization of nucleic acids differing by only a single nucleotide.
Specifically, the present invention relates to the synthesis and use of cationic water-soluble polymers composed of thiophene monomers having the following general formula:
wherein "m" is an integer ranging from 2 to 3; R* is a quaternary ammonium; Y is an oxygen atom or a methylene; and R1 is a methyl group or a hydrogen atom.
The present invention further comprises a method for detecting the presence of negatively charged polymers, comprising the steps of: a) contacting a complementary target to the negatively-charged polymer with a thiophene polymer to form a duplex; b) contacting the duplex with the negatively charged polymer; and c) detecting a change in electronic charge, fluorescence or color as an indication of the presence of the negatively charged polymer.
The negatively charged polymers for the method as described above, may be selected from the group consisting of: acidic proteins, glycosaminoglycans, hyaluronans, heparin, chromatographic substrates, culture substrates and nucleic acids.
The present invention additionally comprises a method for discriminating a first nucleic acid from a second nucleic acid, the second nucleic acid differing from the first nucleic acid by at least one nucleotide, comprising the steps of: a) contacting a complementary target to the first nucleic acid with a thiophene polymer to form a duplex; b) contacting the duplex with the first nucleic acid, resulting in specific hybridization; c) contacting the duplex with the second nucleic acid, resulting in non-specific hybridization; and d) detecting a change in electronic charge, fluorescence or color
Finally, the present invention contemplates a number of specific applications, such as the use of a positively charged polymer comprising a repeating thiophene moiety for detecting the presence of negatively charged polymers, and for purifying negatively charged polymers.
Further scope and applicability will become apparent from the detailed description given hereinafter. It should be understood however, that this detailed description, while indicating preferred embodiments of the invention, is given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the UV-visible absorption spectrum of a 2.4×10-5 M (on a monomeric unit basis) solution of: a) polymer 2; b) polymer 2/X1 duplex; c) polymer 2/X1/Y1 triplex, at 55° C. in 0.1M NaCl/H2O using an optical path length of 1 cm.
FIG. 2 shows the UV-visible absorption spectrum of a 2.4×10-5 M (on a monomeric unit basis) solution of: a) polymer 2; b) polymer 2/X1 duplex; c) polymer 2/X1/X1 mixture, at 55° C. in 0.1M NaCl/H2O.
FIG. 3 shows the UV-visible absorption spectrum of a 2.4×10-5 M (on a monomeric unit basis) solution of: a) polymer 2; b) polymer 2/X1 duplex; c) polymer 2/X1/Y2 mixture, at 55° C. in 0.1M NaCl/H2O.
FIG. 4 shows the specific optical detection of Candida albicans versus Candida dubliniensis amplicons differing by only 2 nucleotides.
FIG. 5 shows a photograph of a polymer 2 --oligonucleotide duplex (solution in the left tube was colored purple-red); a polymer 2--hybridized oligonucleotides triplex (solution in the middle tube was colored yellow); and a polymer 2 with partially hybridized oligonucleotides with two mismatches (solution in the right tube was colored pink).
FIG. 6 shows the formation of a duplex and a triplex between polymer 2 and oligonucleotides.
FIG. 7 shows the fluorescence intensity during specific hybridization.
FIG. 8 shows the fluorescence intensity during non hybridization.
FIG. 9 shows the different steps involved in the electrochemical detection of DNA hybridization.
FIG. 10 shows the Cyclic voltammogram of polymer 1 onto an ITO modified electrode (S=50 mm2) in the case of perfect hybridization (straight line) and in the case of a control blank (dashed line) in 0.1 M NaCl/H2O. Scan rate 100 mV/s (two successive cycles).
FIG. 11 shows the Cyclic voltammogram of polymer 2 onto an ITO modified electrode (S=50 mm2) in the case of perfect hybridization (straight line) and in the case of a control blank (dashed line) in 0.1 M NaCl/H2O. Scan rate 100 mV/s (two successive cycles).
FIG. 12 shows the covalent attachment of a DNA probe onto a conducting surface, its subsequent hybridization with a complementary DNA strand and its revelation with a conducting polymer.
FIG. 13 shows the UV-visible absorption spectrum of a 2.4×10-5 M (on a monomeric unit basis) solution of a) polymer 2, b) polymer 2/X1 duplex, c) polymer 2/X1/Y1 triplex, d) polymer 2/X1/Y2 mixture, and e) polymer 2/X1/Y3 mixture, at 55° C., in 0.1 M NaCl/H2O. Capture probe X1: 5' CATGATTGAACCATCCACCA 3' (SEQ ID NO: 1) and its perfect complementary target Y1: 3' GTACTAACTTGGTAGGTGGT 5' (SEQ ID NO: 2) are a DNA oligonucleotide pair specific for Candida albicans; capture probe X2: 5' CATGATTGAAGCTTCCACCA 3' (SEQ ID NO: 3) and its perfect complementary target Y2: 3' GTACTAACTTCGAAGGTGGT 5' (SEQ ID NO: 4) are a DNA oligonucleotide pair specific for Candida dubliniensis; Y3: 3' GTACTAACTTCGTAGGTGGT 5' (SEQ ID NO: 5) is a complementary target DNA oligonucleotide designed to have one mismatch with both the C. albicans and the C. dubliniensis capture probes.
FIG. 14 shows a cyclic voltammogram of polymer 2 (SH-carbon24-DNA with polymer 2) bound to an ITO modified electrode (50 mm2) in the case of perfect hybridization (X1/Y1; full line) and in the case of two control blanks (X1/X1, dotted line and X1, dashed line) in 0.1 M NaCl/H2O using a scan rate of 100 mV/s.
FIG. 15 shows a cyclic voltammogram of polymer 2 (SH-carbon24-DNA with polymer 2) bound to a gold modified electrode (50 mm2) in the case of perfect hybridization (X1/Y1; full line) and in the case of a control blank (X1, dashed line) in 0.1 M NaCl/H2O. The electrodes were submitted to a -0.4 V potential for 10 minutes before scanning; the scan rate used was 50 mV/s.
FIG. 16 shows a cyclic voltammogram of polymer 3 (SH-carbon24-DNA with polymer 3) bound to an ITO modified electrode (50 mm2) in the case of perfect hybridization (X1/Y1; full line) and in the case of two control blanks (X1, dashed line and silanized ITO (no DNA), dash-dotted line) in 0.1 M NaCl/H2O using a scan rate of 50 mV/s.
FIG. 17 shows the UV-visible absorption spectrum of a 2.4×10-5 M (on a monomeric unit basis) solution of: a) polymer 1/X1 duplex; b) polymer 1/X1/Y1 triplex, at 55° C. in 0.1M NaCl/H2O.
FIG. 18 shows the UV-visible absorption spectrum of a 2.4×10-5 (on a monomeric unit basis) solution of: a) polymer 4; b) X1/Y1/polymer 4 triplex; c) X1/X1/polymer 4 mixture, at 25° C. in 0.1M NaCl/H2O.
FIG. 19 shows the circular dichroism of a 1.2×10-4 M (on a monomeric unit basis) solution of polymer 3/X1/Y1 triplex at 55° C. in 10 mM Tris buffered solution containing 0.1M NaCl.
FIG. 20 shows the UV-visible spectroscopy spectrum of 7.9×10-5 M solutions in 0.1M NaCl and TE, at 55° C. of a) polymer 2; b) duplex (X1+polymer 2); c) triplex (X1+Y1+polymer 2); d) triplex with 2 mismatches (X1+5' TGGTGGATGCATCAATCATG 3' (SEQ ID NO: 6)), triplex with 3 mismatches (X1+5' TGGTGGATACATCAATCATG 3' (SEQ ID NO: 7)), triplex with 5 mismatches (X1+5' TGGTGGAAACAACAATCATG 3' (SEQ ID NO: 8)); e) triplex with 1 mismatch (X1+5' TGGTGGATGCTTCAATCATG 3' (SEQ ID NO: 9)).
FIG. 21 shows the UV-visible spectroscopy spectrum of 7.9×10-5 M solutions in 0.1M NaCl and TE, at 55° C. of a) polymer 2; b) duplex (X1+polymer 2); c) triplex (X1+Y1+polymer 2); d) triplex with 2 mismatches (X1+Y2), (X1+5' TGGTAGATGCTTCAATCATG 3' (SEQ ID NO: 10)), (X1+5' TGGTGGTTGCTTCAATCATG 3' (SEQ ID NO: 11)), (X1+5' TGGTGGATGCTTTAATCATG 3' (SEQ ID NO: 12)), (X1+5' TGGTGGATGCTTCATTCATG 3' (SEQ ID NO: 13)), (X1+5' TGGTGGATGCTTCAATTATG 3' (SEQ ID NO: 14)).
DETAILED DESCRIPTION OF THE INVENTION
Other objects and attendant features of the present invention will become readily appreciated, as the same becomes better understood by reference to the following detailed description of the invention described for the purpose of illustration.
In a broad sense, the invention provides novel cationic, water soluble polythiophene derivatives that produce a clearly interpretable optical (calorimetric, fluorescent or luminescent) or electrical signal, when bound to negatively charged polymers.
The present invention provides for polymers capable of discriminating between specific and non-specific hybridization of nucleic acids differing by only a single nucleotide.
The present invention provides improved research tools. More specifically, a means for detecting nucleic acids from eucaryotic organisms as well as prokaryotic organisms such as Bacteria and Archaea.
The present invention also provides for the development of new nucleic acid detection technology, and more specifically, new detection devices based on the use of the polythiophene derivatives of the present invention.
The present invention further provides for improved clinical diagnostics, that is, the detection of infectious agents, the diagnosis of genetic diseases and tools useful for use in the pharmacogenomics field.
The present invention further provides for improved medico-legal (forensic) diagnostics, more specifically the filiation of people and animals, "forensic" tools and other genetic testing tools.
The present invention also provides for improved plant identification.
The present invention also provides for environmental and industrial screening, more specifically for the detection of genetically modified organisms, the detection of pathogenic agents, alimentary tracability, the identification of organisms of industrial interest (e.g. alimentary, pharmaceutical or chemical fermentation and soil decontamination).
The present invention also provides for polythiophenes having an affinity for negatively charged polymers such as nucleic acids and glycosaminoglycans of natural or synthetic origin, allowing for the purification of these polymers. For example, when a polythiophene is coupled to a solid support, nucleic acids can be purified by affinity and/or ion exchange chromatography.
The present invention also provides for polythiophenes that are thermostable and autoclavable, allowing for a wide range of applications.
The present invention further provides methods and tools by which negatively charged polymers such as acidic proteins (kinesins), glycosaminoglycans (hyaluronans, heparin) and any natural or synthetic negative polymers can be detected or blocked by binding with the polythiophenes.
Advantageously, this novel approach is rapid, specific, sensitive, and highly versatile, yet simple. It is based on the different electrostatic interactions and conformational structural changes between single-stranded or double-stranded negatively-charged oligonucleotides or nucleic acid fragments, and cationic electroactive and photoactive poly(3-alkoxy-4-methylthiophene) derivatives. It allows a single reagent assay procedure for genomic analysis and molecular diagnostics. Furthermore, the above mentioned detection methods can also be used for solutions of nucleic acids, nucleic acids separated by gel electrophoreses, nucleic acids fixed onto solid supports such as glass slides or plates, silicon chips or other polymers.
Set forth below are preferred synthesis schemes for the preparation of the water-soluble, cationic, electroactive and photoactive poly(3-alkoxy-4-methylthiophene)s.
The synthesis of monomer 1 is carried out in a two-step procedure, starting from 3-bromo-4-methylthiophene (Aldrich Co.) (see Scheme 1). The first step is a nucleoplilic substitution reaction onto the thiophene ring catalyzed by CuI, as reported by El Kassmi et al. (El Kassmi et al., A.; Heraud, G.; Buchner, W.; Fache, F.; Lemaire, M. J. Mol. Catal., 72, 299, (1992)). The second step involves a quaternization reaction between the tertiary amine and 1-bromoethane in acetonitrile. (Balanda, P. B.; Ramey, M. B.; Reynolds, J. R. Macromolecules, 32, 3970, (1999)).
Similarly, monomer 2 is prepared from 3-(2-bromoethoxy)-4-methylthiophene (compound 4) and 1-methylimidazole (Aldrich Co.) (see Scheme 2). Compound 4 is prepared according to the procedure developed by Leclerc et al. (Faid, K.; Leclerc, M. J. Chem. Soc., Chem. Commun. , 2761, (1996)). The quaternization reaction between 1-methylimidazole and compound 4, provides the desired monomer imidazolium salt 2. (Lucas, P.; Mehdi, N. E.; Ho, H. A.; Belanger, D.; Breau, L. Synthesis, 9, 1253, (2000)).
Similarly, the synthesis of monomer 3 involves the conversion of 3-thiopheneethanol (compound 5) into its corresponding mesylate protected derivative (compound 6). The quaternization reaction between 1-methylimidazole and compound 6, provides the desired monomer imidazolium salt 3.
The synthesis of monomer imidazolium salt 4 involves a quaternization reaction of compound 4 with 1,2-dimethylimidazole.
A more general procedure reflecting the preparation of cationic thiophene monomers is depicted in scheme 5, wherein "m" is an integer equal to 2 or 3; "Y" is an oxygen atom or a methylene group; and R1 is a hydrogen atom or a methyl group.
When R* is Et3N, Y is an oxygen atom and R1 is a methyl group, then "m" is equal to 3. When R* is 1-methylimidazole, Y is an oxygen atom and R1 is a methyl group, then "m" is equal to 2. When R* is 1-methylimidazole, Y is a methylene group and R1 is a hydrogen atom, then "m" is equal to 2. When R* is 1,2-dimethylimidazole, Y is an oxygen atom and R1 is a methyl group, then "m" is equal to 2.
The inherent chemical and physical properties of imidazole provide for a wide electrochemical window, favorable for electrochemical detection. (Bonhote, P.; Dias, A. P.; Papageorgiou, N.; Kalyanasundaram, K.; Gratzel, M. Inorg. Chem., 35, 1168, (1996)).
All polymers, more specifically the cationic, water soluble, electroactive polymers 1, 2, 3 and 4 (Scheme 6), were synthesized by oxidative chemical polymerization of the corresponding monomers using FeCl3 or K2S2O8 as the oxidizing agent in chloroform. This method of polymerization yields well-defined regio-regular 3-alkoxy-4-methylthiophene polymers (1, 2 and 4) as well as a non-regioregular 3-alkylthiophene polymer (3), having an average molecular weight of about 5 kDa and a polydispersity index of ca. 3. (Chayer, M.; Faid, K.; Leclerc, M. Chem. Mater. , 9, 2902, (1997)). Note that "n" can vary from 3 to about 100. The resulting polymers (using FeCl3 as the oxidizing agent) contain a mixture of anions such as FeCl4.sup.-, Cl.sup.- and Br.sup.-. In order to produce a cationic polymer with only one specific counter anion (e.g. hydrophilic counter anions like Cl.sup.-, Br.sup.-, I.sup.-, CH3SO3.sup.-, etc. or hydrophobic counter anions like BF4.sup.-, CF3SO3.sup.-, PF6.sup.-, etc.), an anionic-exchange reaction is performed by dialysis or precipitation. As expected, all resulting polymers were found to be soluble in aqueous solutions when in the presence of hydrophilic anions.
As any water-soluble cationic polyelectrolytes, the polythiophene derivatives of the present invention can make strong complexes with negatively-charged oligomers and polymers. (Bronich, T. K.; Nguyen, H. K.; Eisenberg, A.; Kabanov, A. V. J Am. Chem. Soc. 122, 8339, (2000)). This complexation results in the formation of complexes having specific optical properties. For instance, at 55° C., aqueous solutions (0.1 M NaCl or 10 mM Tris buffer/0.1M NaCl) of the cationic polymer 2 are yellow (λmax=397 nm). This absorption maximum at a relatively short wavelength is related to a random coil conformation of the polythiophene derivative. (Leclerc, M. Adv. Mater. 11, 1491, (1999)). After the addition of one equivalent (on a monomeric unit basis) of a given oligonucleotide (20-mers), the mixture becomes red (λmax=527 nm) due to the formation of a so-called duplex. After 5 minutes of mixing in the presence of one equivalent of a complementary oligonucleotide, the solution becomes yellow (λmax=421 nm) presumably due to the formation of a new complex (triplex) (FIGS. 1 and 5).
A schematic description of these conformational transitions for both types of polyelectrolytes is given in FIG. 6.
Based on previous studies on thermochromic, solvatochromic and affinitychromic regioregular poly(3-alkoxy-4-methylthiophene)s, (Leclerc, M. Adv. Mater. 11, 1491, (1999)) it is believed that these calorimetric effects are made possible due to the different conformational structure of the conjugated polymer in the duplex (highly conjugated, planar conformation) compared to that observed in the triplex (less conjugated, non-planar conformation) and to a stronger affinity of the conjugated polymer for the double-stranded oligonucleotides (nucleic acids) (1×105 M-1) than that measured for single stranded oligonucleotides (nucleic acids) (5×104 M-1).
In a control experiment it was demonstrated that the addition to the solution of an oligonucleotide identical to that of the capture probe results in no color change (FIG. 2).
In order to verify the specificity of these complexations, two pairs of complementary oligonucleotides (20-mers) differing by only 1 or 2 nucleotides were synthesized (Table 1) and carefully investigated. A slight but distinct change in the UV-visible absorption spectrum is observed in the case of the oligonucleotide target having 2 mismatches. Even with only one mismatch, it is possible to distinguish between a perfect and a non-perfect hybridization (FIG. 13). In this case, the calorimetric difference is mainly based on different kinetics of complexation, since similar yellow aqueous solutions are observed after 30-60 minutes of mixing at 55° C. However, it is possible to stop the hybridization reaction after 5 minutes of mixing, by placing the solutions at room temperature. Following these procedures, stable yellow and orange solutions are obtained (curves c and e) (FIG. 13). The detection limit of this colorimetric method is about 1×1013 molecules of oligonucleotide (20-mers) in a total volume of 100 μL.
Very similar results have been obtained for polymer 1 (FIG. 17) and polymer 4 (FIG. 18) and various oligonucleotides. FIG. 20 shows the UV-Visible absorbance spectrum of polymer 2 when using target oligonucleotides ranging from 0 to 5 mismatches. FIG. 21 illustrates the UV absorbance results of polymer 2 using the target oligonucleotide always having two mismatches at different positions. These results show that the polymer can discriminate between perfectly matched and mismatched hybrids, independently of the nature of the mismatched nucleotide bases, and independently of the position or the length of the mismatches. Moreover, it is even possible to discriminate a single mismatch from multiple mismatches.
TABLE-US-00001 TABLE 1 Synthetic oligonucleotides to study the specificity of hybridization. Oligonucleotides Oligonucleotide specific for specific for Candida albicans Candida dubliniensis X1 X2 5' CATGATTGAACCATCCACC 5' CATGATTGAAGCTTCCACC A 3' A 3' (SEQ ID NO: 1) (SEQ ID NO: 3) Y1 Y2 3' GTACTAACTTGGTAGGTGG 3' GTACTAACTTCGAAGGTGG T 5' T 5' (SEQ ID NO: 2) (SEQ ID NO: 4) Y3 (designed to have one mismatch wit X1 and X2) 3' GTACTAACTTCGTAGGTGGT 5' (SEQ ID NO: 5)
The concentration of a particular sequence region of DNA can be amplified by using a polymerase chain reaction (PCR), and the present calorimetric method can be extended to these PCR products. Indeed, the introduction of the polymerase chain reaction (PCR) has solved the problem of detecting small amounts of DNA and the polymers of the present invention can be used in the identification of PCR products. UV spectroscopic results in the absorbance range of 430-530 nm (FIG. 4) have illustrated the specific optical detection of Candida albicans and Candida dubliniensis amplicons, which differ by only 2 nucleotides, by a polymer 2/X1 duplex. Such detection was carried out in 45 minutes directly from a PCR product at a concentration normally generated in a 100 μL PCR volume (ca. 3×1012 copies). Experimental details on PCR and choice of target sequences for identification of Candida are provided in a co-pending patent application (PCT/CA00/01150).
In addition, as shown in FIG. 19, circular dichroism (CD) measurements reveal an optical activity for polymer 3 in its random coil, a bisignate CD spectrum centered at 420 nm in the triplex, characteristic of a right-handed helical orientation of the polythiophene backbone. Such a right-handed helical structure is compatible with binding of the polymer to the negatively-charged phosphate backbone of DNA. The thermal stability study by UV or CD measurements shows a different thermal stability between a duplex and a triplex, and this property could be extremely useful for more stringent washing conditions.
A fluorometric detection of oligonucleotide hybridization is also possible based on the difference in the fluorescence quantum yield of the positively-charged poly(3-alkoxy-4-methylthiophene) in the random coil (the isolated state) or in the aggregated state. (Chayer, M.; Faid, K.; Leclerc, M. Chem. Mater. , 9, 2902, (1997)). For instance, at 55° C., the yellow appearance of polymer 2 is fluorescent (quantum yield of 0.03) but upon addition of one equivalent of a negatively-charged oligonucleotide, the intensity of the emission spectrum is strongly decreased (quenched). In the case of perfect hybridization, the polymeric triplex gives a stronger emission (FIG. 7). Upon addition of the same oligonucleotide, no hybridization occurs and the solution does not show any change in fluorescent intensity (FIG. 8). Using a laser as the excitation source, very low limits of detection are obtained. It is possible to detect the presence of as few as 3×106 molecules of the complementary oligonucleotide (20-mers) in a volume of 200 μL, which corresponds to a concentration of 2 x 10-14 M. Moreover, by covalently attaching the oligonucleotide to a fluorescent-conjugated polymer, or by using an optimized fluorescence detection scheme based on a high intensity blue diode (excitation source) and a non-dispersive, interference filter-based system, an even more sensitive and more specific detection capability is achieved.
The electrochemical properties of polymers 1 and 2 can be used for the detection of DNA hybridization in aqueous solutions, as shown in FIG. 9. (Youssoufi, H. K.; Garnier, F.; Srivastava, P.; Godillot, P.; Yassar, A. J. Am. Chem. Soc., 119, 7388, (1997); Garnier, F.; Youssoufi, H. K.; Srivastava, P.; Mandrand, B.; Delair, T. Synth. Metals , 100, 89, (1999)). Using the layer by layer deposition techniques (Lvov, Y., Decher, G. & Sukhorukov, G. Assembly of thin layers by means of successive deposition of alternate layers of DNA and poly(allylamine). Macromolecules 26, 5396 (1993); Lvov, Y. M., Lu, Z., Schenkman, J. B., Zu, X. & Rusling, J. F. Direct electrochemistry of myoglobin and cytochrome P450 in alternate layer-by-layer films with DNA and other polyions. J. Am. Chem. Soc. 120, 4073 (1998), the first step required the binding of a capture probe composed of single-stranded oligonucleotide X1 to an ammonium-functionalized indium tin oxide (ITO) surface. (Zammatteo, N; Jeanmart, L.; Hamels, S.; Courtois, S.; Louette, P.; Hevesi, L.; Remacle, J., Anal. Biochem. , 280, 143, (2000); Faid, K. & Leclerc, M. Responsive supramolecular polythiophene assemblies J. Am. Chem. Soc. 120, 5274 (1998)). After rinsing with pure water, the modified electrode so-obtained was dipped and hybridized in the presence of a complementary oligonucleotide Y1. The resulting electrode was revealed with an aqueous solution of positively-charged polymer 1 or 2 (10-4 M on a monomeric basis), which provides a signal that is a function of the amount of DNA present on the surface. As a control experiment, an aqueous solution of oligonucleotide X1 was added to the X1 modified ITO electrode, and then transferred into an aqueous solution of polymer 1 or 2. In this way, these polymers serve as "mass transducers" for the oligonucleotide present in the sample.
The detection of DNA hybridization is further demonstrated by the following two examples using different cationic poly(3-alkoxy-4-methylthiophene)s (polymers 1 and 2). The so-obtained results are illustrated in FIGS. 10 and 11.
In both cases, the maximum anodic current is more important in the case of perfect hybridization as compared to the blank control. In addition, a shift to a higher potential (ca. 40-50 mV) is observed when specific hybridization occurs (38 mV for polymer 4 as compared to 52 mV for polymer 2). The higher oxidation current can be explained by the stronger affinity of the polymers for double-stranded oligonucleotides, whereas the positive shift of oxidation potential is explained by the formation of a less conjugated structure in the case of specific hybridization. This is in agreement with previous optical measurements.
An assay using a smaller electrode [S(surface)=10 mm2] has allowed the detection of 2×1011 molecules of oligonucleotide (20-mers). This very simple electrochemical methodology is already more sensitive, by two orders of magnitude, than the best results obtained with electrochemical methods using oligonucleotide-functionalized conjugate polymers. (Garnier, F.; Youssoufi, H. K.; Srivastava, P.; Mandrand, B.; Delair, T. Synth. Metals, 100, 89, (1999)). Clearly, by decreasing the size of the electrodes and by increasing the size of the target molecules, much lower detection limits should be obtained.
In order to further enhance the specificity of the detection, the oligonucleotide probe can be covalently attached to the polythiophene derivatives
The detection of DNA sequences can also be carried out electrochemically as shown below in Scheme 7.
The DNA probes can be covalently fixed to a conductive surface (FIG. 12). This allows for the linking of a larger number of DNA probes to the surface, improving the specificity and detection limit for a small surface.
The first step involves the modification of the conductive substrate (ITO, SnO2, gold, doped silicon or other conductive substrate) by the covalent attachment of a single-stranded DNA probe. The complementary DNA strand is hybridized and the polymer is captured on the hybridized probe. The observed electrochemical signal is different for a hybridized probe (ds-DNA) versus a non-hybridized probe (ss-DNA). A linker can be used to attach the DNA probe to the conductive surface. The end groups of the linker are such that one end readily reacts with the conductive substrate to form a covalent bond, whereas the other end readily reacts with an "end-modified" (SH, NH2, COOH, etc.) DNA probe, with or without a spacer (carbon24) between the reactive function and the DNA. (Chrisey, L. A.; Lee, G. U.; O' Ferrall, C. E. Nucleic Acids Res. 24, 3031, (1996); Zammatteo, N.; Jeanmart, L.; Hamels, S.; Courtois, S.; Louefte, P.; Hevesi, L.; Remacle, J. J. Anal. Biochem. 280, 143, (2000); Asanov, A. N.; Sarkisov, I. Y.; Oldham, P. B. Part of the SPIE Conference on Clinical Diagnostics Systems and Technologies, 3603, 170, (1999)). The end group of the linker that reacts with the conductive substrate's surface can be a silane derivative, such as an alkoxysilane, or a chlorosilane. The other end group of the linker can be composed of an aldehyde, a carboxylic acid, a primary amine, a succinimide ester moiety or other functional groups capable of reacting with "end-modified" DNA probes, that is, DNA probes having specific end groups, resulting in the formation of covalent bonds with or without the help of coupling agents.
The immobilization of an oligonucleotide by means of a thiol group can be carried out through its 3' or 5' terminal group. Accordingly, DNA was immobilized onto an ITO surface following the literature procedure for immobilizing DNA on a glass surface. (Lenigk, R., Carles, M., Ip, N. Y., Sucher, N. J., Langmuir, 17, 2497 (2001); Rogers, Y. H., Jiang-Baucon, P., Huang, Z. J., Bogdanov, V., Anderson, S., Boyce-Jacino, M. T., Anal. Biochem., 266, 23 (1999); Kumar, A., Larsson, O., Parodi, D., Liang, Z., Nucl. Ac. Res., 28, e71 (2000)).
It is also possible to link "end-modified" DNA probes to various other surfaces such as gold or other metals and metal oxides. (Flink, S.; Frank, C. J. M.; Veggel, D. N. Reinhoudt in Sensors Update, Ed. H. Baltes, W. Gopel, J. Hesse, vol. 8, Chap. 1 (2001)). Non-metallic surfaces such as beads, glass slides, optical fibers or any other suitable non-metallic solid support, are also possible. The immobilization of DNA onto a gold surface is accomplished following known published techniques, and the density of the attached probes can vary depending on the concentration and reaction times. (Peterson, A. W., Heaton, R. J., Georgiadis, R. M., Nucl. Ac. Res., 29, 5163 (2001); Steel, A. B., Levicky, R. L., Heme, T. M., Tarlov, M. J., Biophys. J, 79, 975 (2000). The hybridization efficiency can be optimized by heating the substrate before adding the complementary strand. (Peterson, A. W., Heaton, R. J., Georgiadis, R. M., Nucl. Ac. Res., 29, 5163 (2001); Peterlinz, K. A., Georgiadis, R. M., Heme, T. M., Tarlov, M. J., J. Am. Chem. Soc., 119, 3401 (1997)). (Peterson, A. W., Heaton, R. J., Georgiadis, R. M., Nucl. Ac. Res., 29, 5163 (2001); Peterlinz, K. A., Georgiadis, R. M., Heme, T. M., Tarlov, M. J., J. Am. Chem. Soc., 119, 3401 (1997)). Polymer deposition can be optimized by dipping the substrate vertically into a polymer solution, and by varying the salt concentration and the temperature of the polymer solution. The washes can be further optimized to limit the contribution of the polymer with the non-hybridized probe.
The signal observed for a triplex should be stronger than that observed for a duplex, with possibly a small shift towards higher potentials when hybridization occurs. The amount of DNA is higher in the case of a triplex, which implies the presence of a higher amount of negative charges. It is suspected that there might be two equivalents of polymer (positive charge) binding in the case of a triplex. Moreover, in the case where only one equivalent of polymer is bound to DNA, the polymer on a non-hybridized probe (ss-DNA) is more easily washed off, as compared to a polymer bound to DNA on a hybridized probe (ds-DNA), since it is less stable at high salt content or elevated temperatures. By washing off essentially all of the polymer from the duplex while leaving the triplex intact, a signal coming directly from the triplex (hybridized probe+polymer) is assured. Finally, targets having mismatches will give a different signal and possibly a lower current, due to less perfect hybridization.
The electrochemical detection of DNA hybridization is further demonstrated by using polymer 2 and polymer 3, as illustrated in FIGS. 14-16.
General Procedure for the Aromatic Nucleophilic Substitution on Thiophene; Synthesis of Compound 3
Sodium hydride (0.4 g, 15 mmol) was added between 0 and 10° C. under nitrogen to a solution of N,N-diethylpropanolamine (2.0 g; 15.2 mmol) in 50 mL of DME, and the resulting mixture stirred at ambient temperature for 20 minutes. 3-Bromo-4-methyl thiophene (2.0 g, 11.3 mmol) dissolved in 20 mL of DME (20 mL) and CuI (1.07 g, 5.65 mmol) were then added to the reaction mixture. The mixture was subsequently stirred overnight at 95° C., while under nitrogen, diluted with methylene chloride and filtered. The organic phase was washed three times with water, dried over MgSO4 and evaporated. The crude product was purified by chromatography on silica gel using CH2Cl2 as the eluent, followed by the use of MeOH.
Compound 3: Yield 26% ; 1H NMR (CDCl3) δ: 1.04 (t, 6H); 1.93 (m, 2H); 2.09 (s, 3H); 2.57 (m, 6H), 3.97 (t, 2H); 6.14 (d, 1H); 6.81 (d, 1H); 13C NMR (CDCl3) δ: 11.69; 12.50; 26.84; 46.91; 49.26; 68.19; 95.88; 119.57; 129.05; 156.04; MS m/e: Calcd. For C12H21N1O1S1; 227.1344; Found: 227.1347.
General Procedure for Quaternization Reaction: Synthesis of Monomer 1
1-Bromoethane (6 mL, 80.4 mmol) was added to a solution of compound 3 (0.4 g, 1.8 mmol) in 60 mL of acetonitrile. The reaction mixture was stirred at 70° C. under nitrogen for 3 days. After evaporation of the acetonitrile, the crude product was crystallized from ethyl acetate as a colorless powder.
Monomer 1: Yield 100%; m.p. 145-147° C.; 1H NMR (CDCl3) δ: 1.41 (t, 9H); 2.06 (s, 3H); 2.31 (m, 2H); 3.57 (m, 8H), 4.12 (t, 2H); 6.24 (d, 1H); 6.84 (d, 1H); 13C NMR (CDCl3) δ: 7.87; 12.61; 22.55; 53.61; 54.66; 65.80; 97.18; 120.28; 128.26; 154.77.
Synthesis of Monomer 2
1-Methyl-imidazole (1.0 mL, 12.3 mmol) was added to a solution of product 4 (0.54 g, 2.46 mmol) dissolved in CH3CN (35 mL). The resulting reaction mixture was stirred at 70° C. for two days. After evaporation of the solvent, the crude product was washed twice with warm ethyl acetate and twice with diethyl ether at room temperature to provide monomer 2 as a pure white solid compound.
Monomer 2: Yield 88%; 92-94° C.; 1H NMR (CDCl3) δ: 2.05 (s, 3H); 4.09 (s, 3H); 4.38 (t, 2H); 4.90 (t, 2H), 6.27 (d, 1H); 6.82 (d, 1H); 7.63 (s, 1H); 7.68 (s, 1H); 10.24 (s, 1H), 13C NMR (CDCl3) δ: 12.81; 36.76; 49.36; 68.01; 97.92; 120.68; 123.26; 123.30; 128.35; 137.51; 154.23.
Synthesis of Monomer 3
Methanesulfonyl chloride (2.4 mL; 31.2 mmol) was added dropwise to a solution of 3-thiopheneethanol (2.0 g; 15.6 mmol) and triethylamine (4.3 mL; 31.2 mmol), in dichloromethane (50 mL). The reaction mixture was stirred at room-temperature for two hours. The organic phase was washed with a NaHCO3 solution, followed by several washings with water, and was finally dried with MgSO4 and concentrated. The crude product was purified by silica gel chromatography using CH2Cl2/hexane (1/1) as the eluent to yield compound 6 (59%); 1H NMR (300 MHz, CDCl3, 25° C., TMS): δ=2.87 (s, 3H); 3.08 (t, 2H); 4.40 (t, 2H); 6.98 (d, 1H); 7.09 (d, 1H); 7.29 (dd, 1H). 13C NMR (300 MHz, CDCl3, 25° C., TMS): δ=29.99; 37.19; 69.59; 122.24; 125.98; 127.98; 136.44.
1-Methyl imidazole (0.4 g; 4.85 mmol) was added to a solution of compound 6 ( 0.2 g; 0.97 mmol) in toluene (20 mL). The reaction mixture was stirred at 94° C. for two days. Following evaporation of the solvent, the crude product was washed with warm ethyl acetate to yield monomer 3 as a liquid.
Monomer 3: Yield (95%); 1H NMR (300 MHz, CDCl3, 25° C., TMS): δ=2.72 (s, 3H); 3.19 (t, 2H); 3.90 (s, 3H); 4.49 (t, 2H); 6.95 (d, 1H); 7.06 (d, 1H); 7.24 (m, 2H); 7.33 (s, 1H); 9.68 (s, 1H). 13C NMR (300 MHz, CDCl3, 25° C., TMS): δ=30.77; 36.10; 39.67; 50.06; 122.24; 122.81; 123.01; 126.46; 127.66; 136.08; 137.80.
Synthesis of Monomer 4
The quaternization reactions were carried out following the procedure described in example 3.
Monomer 4: Yield 85%; 1H NMR (300 MHz, CDCl3, 25° C., TMS): δ=2.02 (s, 3H); 2.89 (s, 3H); 3.96 (s, 3H); 4.39 (t, 2H); 4.85 (t, 2H); 6.27 (d, 1H); 6.83 (d, 1H); 7.57 (d, 1H); 7.96 (d, 1H).
General Procedure for the Chemical Polymerization: Synthesis of Polymer 1
To a solution of iron trichloride (0.94 g, 5.8 mmol) in chloroform (23 mL) under nitrogen, a solution of monomer 1 (0.487 g, 1.4 mmol) in chloroform (15 mL) was added dropwise. The mixture was stirred at room temperature for a period of 2 days. The reaction mixture was evaporated to dryness and the crude product washed quickly with methanol, and dissolved in excess of acetone, and precipitated by the addition of an excess of tetrabutylammonium chloride or tetrabutylammonium bromide. The black-red polymer was dissolved in methanol and dedoped by adding a few drops of hydrazine. The final solution was evaporated. The resulting polymer was washed several times with a saturated solution of tetrabutylammonium chloride or tetrabutylammonium bromide in acetone, and by Soxlet extraction with acetone over a period of 6 hours, and then dried under reduced pressure to yield polymer 1 (0.32 g, 66%).
General Procedure for Optical Detection
i) Synthetic Oligonucleotides
In a quartz UV cuvette, 100 μL (7.47×10-8 repeat units (RU) of positive charges) of a solution of polymer 2 were added to an aqueous solution (3 mL) containing either 0.1M NaCl or 10 Mm Tris buffer plus 0.1M NaCl (pH=8). The mixture was heated at 55° C. for 5 min and had a yellow appearance. 12 μL (7.47×10-8 RU of negative charges) of oligonucleotide solution (capture probe) were then added and the resulting red solution kept at 55° C. for an additional 5 minutes. The appropriate oligonucleotide target was added to the solution at 55° C. over 5 minutes. A final yellow color is indicative of a positive result, meaning that perfect hybridization has taken place. On the other hand, a red or a red-pink color is representative of non specific or partial hybridization (two mismatches), respectively (FIGS. 1-3, respectively).
ii) PCR Products
The amplicon (double stranded 149 base pairs) from the PCR product was pre-purified by QIAQUICK DNA purification columns purchased from Qiagen (Valencia, Calif.). H2O (90 μL) was added to a centrifuge tube followed by the addition of 6.7 μL (5.03×10-9 mol of positive charge) of a solution of polymer 2, followed by the addition of the oligonucleotide capture probe Y1 (2 μL; 5.03 10-9 mol of negative charge) and finally, by the addition of NaCl 1M (20 μL). The resulting mixture was heated at 50° C. for 10 min. The purified PCR product was freshly denatured, cooled in ice water and then added to the above solution. The hybridization reaction was kept at 50° C. for 35 min and the color change observed either visually or by UV measurement (FIG. 4).
General Procedure for Fluorescent Detection
A procedure identical to that described for optical detection was employed, with the exception of the use of a fluorospectrometer. The fluorescent intensity of a "duplex" (association between a positively charged polymer and an oligonucleotide capture probe) was weak or insignificant (practically zero) due to the fluorescent-quenching property of the aggregated form of the polymer. When perfect hybridization does occur, the fluorescent signal becomes more significant (FIG. 7).
General Procedure for Electrochemical Detection
The electrochemical test for hybridization was performed in conjunction with a control blank. 60 μL (1.44×10-8 mol of negative charge) of captured oligonucleotide Y1 were deposited on the aminated ITO electrode (S=50 mm2) at ambient temperature for 5 min. After washing with water, 60 μL (1.44×10-9 mol of negative charge) of the target oligonucleotide X1 were added and the hybridization carried out at 55° C. over 20 minutes. The electrode was then cooled to room temperature over 10 min and washed twice with 0.3 M NaCl, 0.03 M NaOAc and 0.1% SDS (pH 7) and water. 100 μL (1×10-8 mol of positive charge) of a solution of polymer 1 or 2 was spread on the modified electrode for 5 min, followed by washing with CH3CN/H2O (1/4) and water. Cyclic voltammograms were performed in aqueous 0.1 M NaCl solutions (FIGS. 10 and 11).
General Procedure for the Preparation of Electrodes Substituted with Covalent Probes
i) Covalent Attachment of DNA Probes to an ITO Electrode (Polymer 2)
ITO slides are sonicated in hexanes (10 min), methanol (10 min) and ultrapure water (10 min), and are then treated with an aquaregia solution (H2O2/H2O/NH4OH, 1/5/1) at 40° C. over a 30 minute period. The resulting slides are rapidly washed with water, followed by sonication in water and in acetone, then dried with air, nitrogen or argon, and heated to 110° C. for 2 to 10 min. Following this, the slides are submerged for three hours, while under an inert atmosphere, in an acidified ethanol solution (1 mM acetic acid in 95% ethanol) containing 5% mercaptopropyltrimethoxysilane, followed by sonication in fresh ethanol (95%) and in ultrapure sterile water. Finally, the slides are heated at 110° C. for at least one hour, and cooled to room temperature before modification with DNA.
The washes, following DNA deposition, hybridization and polymer deposition, are carried out using an orbital shaker, unless otherwise stated. DNA attachment is carried out using a solution of SH-carbon24-DNA in sodium citrate buffer (30 mM, pH=4, 50 μL) which is deposited onto each ITO slide, each spot forming a circle of about 1 cm in diameter. The deposition reaction is performed in a humidified chamber over a period of 20-24 hours. Any non-deposited DNA is drained off, and the slides washed with 5×SSC+0.1% Tween 20, followed by washings with 1×SSC +0.1% Tween 20 and NaCl 0.1M.
ii) Hybridization (ITO, Polymer 2)
50 μL of RC-DNA (Y1) or of probe X1 (blank test) (25 μM in 2×SSC), are deposited onto the DNA spot. The slides are then heated to 55° C. for about 3 hours while in a humid atmosphere, and then cooled to room temperature for 15 minutes. Any non-reacted DNA is drained off and the slides washed with 2×SSC, NaCl (0.1M), with 1×SSC+0.1% Tween 20 and with NaCl (0.1M).
iii) Polymer Deposition (ITO, Polymer 2)
Polymer 2 is deposited onto the electrodes. The slides are dipped vertically over a period of 5 minutes in a solution of polymer 2 (2 mL of 10-4 M in 0.01M NaCl) at room temperature. The slides are then dipped in 2 mL of a NaCl solution (0.01M), followed by dipping in 2 mL of a CH3CN/H2O (1/4) solution and finally, by dipping in 2 mL of a NaCl solution (0.01M).
iv) Hybridization (ITO, Polymer 3)
30 μL of Y1 (2.5 μM/NaCl 0.1M) are inserted into hybridization chambers which are placed onto the surface of the ITO electrode. The slides are heated for about two hours at 55° C. while in a humid atmosphere, and then cooled to room temperature. The hybridization chambers are removed and the slides washed with a 0.1M NaCl solution and finally dried with argon.
v) Polymer Deposition (ITO, Polymer 3)
Polymer 3 is deposited onto the electrodes. A 30 μL aqueous solution of polymer 3 (10-4M) is inserted into hybridization chambers which are then placed onto the surface of the ITO electrode. The slides are heated at 55° C. for 20 minutes. The hybridization chambers are removed and the slides washed with a first portion of a 0.8M NaCl solution at 55° C., then washed with a new portion while cooling to room temperature. The slides are finally rinsed at room temperature with a 0.1M NaCl solution.
vi) Covalent Attachment of DNA Probes to a Gold Electrode (Polymer 2)
Gold slides are rinsed sequentially with hexanes, methanol and water. The plates are then treated with a pyranah solution (H2O2 30%/H2SO4 concentrated; 30/70) over a period of about 15 minutes. The slides are then thoroughly washed with nanopure sterile water, and dried with argon.
DNA deposition is performed under inert atmosphere using 50 μL of a solution of SH-carbon24-DNA (25 μM in 1M phosphate buffer (pH=7); K2HPO4+KH2PO4). The solution is deposited onto a 1 cm2 surface, and treated for about 16 hours under inert atmosphere. Any non-reacted DNA is then drained off, and the slides sequentially washed with H2O, 2×SSC, and sterile water.
Polymer 2 is deposited onto the electrodes. A 50 μL solution of polymer 2 (10-5 M in 0.1M NaCl) is deposited onto the DNA spot and the slides heated to 55° C. for about 30 minutes. The slides are then washed repeatedly with a NaCl solution (0.1M) at room temperature.
Note that the order of additions does not have to follow that described in the above example, that is, a complementary (or a non-complementary or with mismatches) DNA strand is added to a covalently linked DNA strand, followed by the addition of a polymer solution. The polymer solution may be added prior to the addition of the complementary (or a non-complementary or with mismatches) DNA strand.
The Use of the Polymers of the Present Invention to Purify Nucleic Acids and Other Such Negatively Charged Molecules
The polymers described in the present invention have high affinity for negatively charged molecules, especially nucleic acids. In addition, they are soluble in aqueous solution and very stable under a wide range of temperatures. Therefore, it is possible to use these properties to purify nucleic acids and other negatively charged molecules. Chromatographic separation would involve the following steps: (1) immobilizing the polymers; (2) applying the analyte to be separated onto the immobilized polymers under conditions such that electrostatic interactions are possible between the analyte and the polymer; and (3) eluting the analyte by applying conditions where electrostatic interactions between the analyte and the polymer are not favored.
Immobilization of the polymer can be achieved by covalently coupling the polymer to a suitable solid support such as glass beads, or to beads made of other types of polymers. Alternatively, coupling to a solid support could be achieved via electrostatic interactions, such as that demonstrated in Example 10. In the latter case, where a capture oligonucleotide is covalently attached to a solid support, the polymer would be expected to be eluted along with the analyte in the final elution step since it is attached only by electrostatic bonds. Color changes could even be used to monitor the chromatographic process.
Conditions for binding the analyte to the polymer would preferably involve solutions of low ionic charge, such as water, 0.1 M NaCl and 10 mM Tris buffer/0.1 M NaCl. Different washing conditions can be applied to remove any unwanted fractions of the analyte. pH changes could alternatively be used to obtain conditions for binding the analyte to the polymer.
Conditions for eluting the analyte involves solutions of high ionic charge, such as 1.0 M NaCl solution or any other solution comprising a sufficiently high counter ion concentration capable of competing for the electrostatic interactions with the polymer. Again, pH changes could alternatively be used to obtain conditions for eluting the analyte.
Detection of Messenger RNA
Purified, in vitro transcribed, polyadenylated messenger RNA of Arabidopsis thaliana gene coding for NAC1, was purchased from Stratagene. Perfectly matched complementary DNA oligonucleotide N1 (5' CGAGGCTTCCATCAATCTTA 3' SEQ ID NO: 15) was synthesized by phosphoramidite chemistry on a Perkin-Elmer 391 synthesizer. Unrelated Y2 DNA oligonucleotide was obtained in the same manner. All reagents and liquid handling material coming into contact with the RNA were either certified RNAse-free or treated with diethylpyrocarbonate (J. Sambrook and D. W. Russel, Molecular Cloning, A Laboratory Manual, CSHL Press, 2001) to inactivate RNAses. Fluorescence measurements were performed at 42.5° C. on a Variant Cary Eclipse spectrofluorometer with excitation set at 420±10 nm and fluorescence emission measured at 530±5 nm, applying 1000 volts to the detector.
A duplex between polymer 2 and oligo N1 was formed by contacting 8.66×1013 copies of oligo N1 with the equivalent amount of positive charges of polymer 2. The reaction was carried out at room temperature for thirty seconds in 2 μL of water. One μl of the duplex mixture was diluted in 2 mL of water and put into a quartz cuvette for measurement of the initial fluorescence signal. Thereafter, a triplex was formed at 42.5° C. by adding and mixing 8.66×1013 copies of heat treated (2 minutes at 95° C.) NAC1 messenger RNA. Fluorescence of the triplex was measured. A similar duplex and triplex was also made using the DNA oligonucleotide Y2, which has no significant homology with the sequence of the NAC1 messenger RNA.
The fluorescence was significantly higher for the N1/polymer 2/NAC1 triplex than for the Y2/polymer 2/NAC1 triplex, thereby showing the ability of polymer 2 to distinguish between specific and nonspecific hybridization of 20 mers DNA oligonucleotides with messenger RNA.
In conclusion, a novel methodology has been developed that allows the detection of nucleic acids by simple optical and electrochemical means. This rapid, selective, and versatile method does not require any chemical reaction on the probes or the analytes, and is based on different electrostatic interactions and conformational structural changes between cationic poly(3-alkoxy-4-methylthiophene) derivatives and single-stranded oligonucleotides or double-stranded (hybridized) nucleic acid fragments. The present polymer-based technology is simple and specific and provides a flexible platform for the rapid detection of nucleic acids.
The polythiophenes are thermostable and autoclavable, hence allowing for a wide range of applications.
The terms and descriptions used herein are preferred embodiments set forth by way of illustration only, and are not intended as limitations on the many variations which those of skill in the art will recognize to be possible in practicing the present invention, as defined in the following claims.
15120DNAArtificial SequenceOligonucleotide 1catgattgaa ccatccacca 20220DNAArtificial SequenceOligonucleotide 2gtactaactt ggtaggtggt 20320DNAArtificial SequenceOligonucleotide 3catgattgaa gcttccacca 20420DNAArtificial SequenceOligonucleotide 4gtactaactt cgaaggtggt 20520DNAArtificial SequenceOligonucleotide 5gtactaactt cgtaggtggt 20620DNAArtificial SequenceOligonucleotide 6tggtggatgc atcaatcatg 20720DNAArtificial SequenceOligonucleotide 7tggtggatac atcaatcatg 20820DNAArtificial SequenceOligonucleotide 8tggtggaaac aacaatcatg 20920DNAArtificial SequenceOligonucleotide 9tggtggatgc ttcaatcatg 201020DNAArtificial SequenceOligonucleotide 10tggtagatgc ttcaatcatg 201120DNAArtificial SequenceOligonucleotide 11tggtggttgc ttcaatcatg 201220DNAArtificial SequenceOligonucleotide 12tggtggatgc tttaatcatg 201320DNAArtificial SequenceOligonucleotide 13tggtggatgc ttcattcatg 201420DNAArtificial SequenceOligonucleotide 14tggtggatgc ttcaattatg 201520DNAArtificial SequenceOligonucleotide 15cgaggcttcc atcaatctta 20
Patent applications by Hoang Anh Ho, Sainte-Foy CA
Patent applications by Mario Leclerc, Quebec CA
Patent applications by Maurice Boissinot, Saint-Augustin-De-Desmaures CA
Patent applications in class Involving nucleic acid
Patent applications in all subclasses Involving nucleic acid