Patent application title: BIVM (BASIC, IMMUNOGLOBULIN-LIKE VARIABLE MOTIF-CONTAINING) GENE, TRANSCRIPTIONAL PRODUCTS, AND USES THEREOF

Inventors: Gary W. Litman (Gulfport, FL, US) Noel A. Hawke (Durham, NC, US) Jeffrey A. Yoder (St. Petersburg, FL, US) Donna D. Eason (Bradenton, FL, US)
Assignees: University of South Florida
IPC8 Class: AA61K39395FI
USPC Class: 4241391
Class name: Binds antigen or epitope whose amino acid sequence is disclosed in whole or in part (e.g., binds specifically-identified amino acid sequence, etc.)
Publication date: 09/17/2009
Patent application number: 20090232824

Abstract:

The subject invention provides polynucleotide sequences, designated BIVM, and transcriptional/translational products obtained from the polynucleotide sequences of the invention. The subject invention also provides polynucleotide and polypeptide sequences provided by SEQ ID NOs: 1-28. Also provided are methods of detecting the presence of BIVM nucleic acids or polypeptides in samples suspected of containing BIVM genes, BIVM transcriptional products, or BIVM translational products. These methods are also useful for the detection of BIVM orthologs. Other embodiments provide polypeptide and/or nucleic acid vaccines for the induction of an immune response to in an individual. Kits for detecting the presence of BIVM genes, orthologs thereof, BIVM polypeptides, or BIVM transcriptional products are also provided.

Claims:

1. A method of inducing an immune response in an individual comprising administering a composition comprising a carrier and a polypeptide comprising SEQ ID NO: 2 or a biologically active fragment of SEQ ID NO: 2 to an individual in an amount sufficient to induce an immune response.

2. The method according to claim 1, wherein said biologically active fragment comprises at least five consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

3. The method according to claim 1, wherein said biologically active fragment comprises at least ten consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

4. The method according to claim 1, wherein said biologically active fragment comprises at least fifteen consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

5. The method according to claim 1, wherein said composition comprises SEQ ID NO: 2 and a carrier.

6. The method according to claim 1, wherein said biologically active fragment comprises at least twenty consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

7. The method according to claim 1, wherein said biologically active fragment comprises at least twenty-five consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

8. The method according to claim 1, wherein said biologically active fragment comprises at least thirty consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

9. An isolated polypeptide comprising SEQ ID NO: 2 or a biologically active fragment thereof, said biologically active fragment comprising at least five consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

10. The isolated polypeptide according to claim 9, wherein said biologically active fragment comprises at least ten consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

11. The isolated polypeptide according to claim 9, wherein said biologically active fragment comprises at least fifteen consecutive amino acids of SEQ ID NO: 2 fused to a heterologous protein sequence.

12. A composition comprising a carrier and a polypeptide according to claim 9.

13. An isolated antibody that specifically binds to SEQ ID NO: 2.

14. A composition comprising a carrier and an antibody according to claim 13.

Description:

CROSS REFERENCE TO RELATED APPLICATIONS

[0001]The application is a continuation of U.S. application Ser. No. 11/415,536, filed May 2, 2006, now allowed, which is a divisional of U.S. application Ser. No. 10/417,476, filed Apr. 16, 2003, now U.S. Pat. No. 7,038,030, which claims priority to U.S. Provisional Application Ser. No. 60/373,146, filed Apr. 16, 2002, the disclosures of which are hereby incorporated by reference in their entireties, including all figures, nucleic acid sequences, amino acid sequences, and tables.

[0003]The Sequence Listing for this application is labeled "Seq-List.txt, was created on Apr. 14, 2009, and is 251 KB. The entire contents of the sequence listing is incorporated herein by reference in its entirety.

BACKGROUND OF INVENTION

[0004]Considerable uncertainty remains with regards to the total number of human genes. Initial interpretations of genomic sequences resulted in estimates that placed the numbers of genes in man in the range of 30,000 to 40,000 (Lander, E. S., et al. [2001] "Initial Sequencing and Analysis of the Human Genome," Nature, 409:860-921; Ventner, J. C., et al. [2001] "The Sequence of the Human Genome," Science, 291:1304-51). Subsequent re-examination of the sequence data suggests the number of genes in the human genome is likely to be between 65,000 and 75,000 (Wright, F. A., et al. [2001] "A Draft Annotation and Overview of the Human Genome," Genome Biology 2:1.1-1.39). Predictions of 35,000 to 120,000 genes have been projected on the basis of EST sequences (Ewing, B., et al. [2000] "Analysis of Expressed Sequence Tags Indicates 35,000 Human Genes," Nature Genet. 25:232-234; Liang, F., et al. [2000], "Gene Index Analysis of the Human Genome Estimates Approximately 120,000 Genes," Nature Genet. 25:239-240). New genes continue to be recognized through inspection of genomic sequences as well as through a variety of different biochemical, immunological and other directed approaches.

[0005]The immunoglobulin superfamily (IgSF) represents a particularly large and extensively diversified family of genes (Barclay, A. N., et al. [1997] The Leucocyte Antigen FactsBook, Academic Press, San Diego). Each IgSF member encodes at least one Ig that consists of ˜100 amino acid residues that are arranged in two β sheets, which are comprised of anti-parallel β strands that are linked by an intrachain disulfide. Although the majority of genes in the IgSF function in the immune response, other IgSF genes are involved with cell-adhesion or growth factor recognition. IgSF domains are the most abundant domain type found in leukocyte membrane proteins.

[0006]In the course of an electronic EST database search for novel human genes encoding Ig domains, we identified an anonymous EST (IMAGE 785450; GenBank AA449273) (Hawke, N. A., et al. [1999] "Expanding Our Understanding of Immunoglobulin, T-cell Antigen Receptor, and Novel Immune-Type Receptor Genes: a Subset of the Immunoglobulin Gene Superfamily," Immunogenetics 50:124-133) and cloned the corresponding full-length cDNA. The predicted structure of the protein encoded by this gene, which is termed BIVM (basic, immunoglobulin-like variable motif-containing), includes short peptide motifs characteristic of an Ig variable (V) region, one of the subtypes of Ig domains. However, it lacks significant sequence identity to any group of proteins heretofore described.

[0007]We have determined the sequence of BIVM cDNA in species representative of critical points in phylogeny, examined the intracellular distribution of a recombinant form of BIVM, characterized its expression patterns in various tissues at different times in development, and defined other features of the gene that further emphasize its unique character. In addition, we have identified a BIVM-like gene in the protozoan parasite, Giardia lamblia.

BRIEF SUMMARY

[0008]The subject invention provides polynucleotide sequences, designated BIVM, and transcriptional/translational products obtained from the polynucleotide sequences of the invention (SEQ ID Nos:1-28). The subject invention also provides methods of detecting the presence of BIVM nucleic acids, transcriptional products, or polypeptides in samples suspected of containing BIVM genes. These methods are also useful for the detection of BIVM orthologs. Other embodiments provide polypeptide and/or nucleic acid vaccines for the induction of an immune response. Kits for detecting the presence of BIVM genes, orthologs thereof, BIVM polypeptides, or BIVM transcriptional products obtained from the polynucleotide sequences are also provided.

BRIEF DESCRIPTION OF THE TABLES AND DRAWINGS

[0009]The file of this patent contains at least one drawing executed in color. Copies of this patent with color drawings will be provided by the Patent and Trademark Office upon request and payment of the necessary fee.

[0010]Table I. Exon-intron organization of human BIVM. Three alternative splice donors in the 5' untranslated region are designated A₁, A₂, and A₃. Nucleotide positions are relative to FIG. 1, intron length and splice donor/acceptor sequences are shown. Coding sequence is in upper case.

[0011]FIGS. 1A-1C. Human BIVM. The nucleotide sequence (SEQ ID NO: 1) and predicted amino acid translation product (SEQ ID NO: 2) of a human BIVM transcript. Translational start and stop codons are in reverse text. RNA splice junctions are underlined (see Table I). Nucleotides at 5' ends, defined by analyses of RACE products, are boxed. Nucleotide numbering is on the right; amino acid numbering is on the left. The M1(GX₆C), M2 (WFRQ), M3a and M3b (YFC and YHC) motifs are shaded. The Alu sequence in the 3' untranslated region is in lower case.

[0012]FIG. 2. Predicted genomic organization of human BIVM. BIVM consists of nine coding exons (exons 1-9) and two 5' untranslated region exons (A and B). Alternative splice donor sites are present within exon A (see Table I); transcripts have been identified that include exon A, but not exon B. The CpG island is denoted by a solid bar, the Alu sequence is denoted by a hatched bar, and the location of the sequence-tagged site (STS) marker, WI-5740, is indicated (see also FIG. 1A).

[0013]FIGS. 3A-3B. BIVM is well conserved among deuterostomes. ClustalW alignment of the human BIVM peptide sequence (BIVM.Hs; (SEQ ID NO: 2)) with orthologous sequences from mouse (BIVM.Mm; (SEQ ID NO: 27)), chicken (BIVM.Gg; (SEQ ID NO: 8)), Xenopus (XBIVM; (SEQ ID NO: 5)), zebrafish (BIVM.Dr; (SEQ ID NO: 11)), and sea urchin (SpBIVM; (SEQ ID NO: 13)). The sea urchin sequence lacks a stop codon and therefore is predicted to encode a longer polypeptide (indicated by . . . ). The M1, M2, M3a and M3b motifs are indicated. The highly conserved domain within BIVM is indicated with arrowheads. Identical residues are shown in reverse text (black), similarities are shaded (gray). Gaps introduced to maintain/maximize alignment are indicated with (-).

[0014]FIG. 4. Syntenic relationship between the human BIVM region and the mouse genome. The relative locations of human BIVM and flanking genes on chromosome 13q32-33; known corresponding chromosomal map positions are indicated for mouse. Transcription direction is indicated with arrows. Approximate distances between genes (if known) are indicated.

[0015]FIGS. 5A-5F. Expression of BIVM. RNA blots of BIVM expression from (A) human tissues and cell lines, (B) mouse embryos and somatic tissues, (C) Xenopus embryos and kidney, and (D) sea urchin embryos. Approximately 2 μg of polyA+ RNA/track was analyzed in human and mouse; ˜10 μg of total RNA/track was analyzed in Xenopus and sea urchin. Actin is used as a loading control with human and mouse blots; 18S ribosomal RNA is used as a loading control with Xenopus and sea urchin blots. Real time PCR analysis of BIVM expression in (E), developing zebrafish embryos and adult tissues, and in (F) chicken bursa at various stages of embryonic development. The quantity of BIVM (designated on the left) is relative and normalized (see Methods). Note that the level of zebrafish BIVM expression in the 0 hpf embryo is approximately 10 times the level detected at 6 hpf. Time points in the analysis of bursa are days of embryonic life (e.g. E12) and chicken embryonic fibroblasts (CEFs) were included as a control. Days post coitus=dpc, stage=st., hour post fertilization=hpf, days post fertilization=dpf and intestine=intest.

[0016]FIGS. 6A-6J. BIVM localizes to the nucleus and the cytoplasm. (A) Western analysis of whole cell lysates from pIRES2-EGFP (EGFP), pBIVM-N2/EGFP (N2/EGFP) and pBIVM-K1/EGFP (K1/EGFP) transfected Cos-7 cells. Recombinant BIVM is detected with an anti-V5 antibody. EGFP is shown as a transfection and loading control. Note that only a single protein corresponding to the 5' ATG is generated from the endogenous transcript (pBIVM-N2); protein synthesis is increased by modification of the translational start site (pBIVMK1). Size standards are indicated. (B) Western analysis of nuclear and cytoplasmic fractions from pBIVM-K1/EGFP transfected Cos-7 cells. OCT-1 (Pombo, A., et al. [1998] "Regional and Temporal Specialization in the Nucleus: A Transcriptionally-Active Nuclear Domain Rich in PTF, Oct1 and PIKA Antigens Associated with Specific Chromosomes Early in the Cell Cycle," EMBO J. 1768) and HSP90 (Perdew, G. H., et al. [1991] "Evidence that the 90-kDa Heatshock Protein (HSP90) Exists in Cytosol in Heteromeric Complexes Containing HSP70 and Three Other Proteins with Mr 63,000, 56,000, and 50,000," J Biol Chem 6708) are nuclear and cytoplasmic markers, respectively. (C-J) Immunocytochemical localization of BIVM. Cos7 cells transiently transfected with pBIVM-K1 were analyzed by conventional fluorescent microscopy. Recombinant BIVM (green), actin (red), nuclei (blue), and overlayered images are shown. Note that levels of nuclear BIVM vary (compare C to G).

[0017]FIGS. 7A-7C. Giardia BIVM-like sequence. (A) The nucleotide sequence (SEQ ID NO: 14) and predicted amino acid translation product (SEQ ID NO: 15) of a Giardia lamblia BIVM-like (BIVML) transcript. Translational start and stop codons are in reverse text. Numbering is as in FIG. 1. Grey shading indicates conserved motifs. A sequence resembling predicted giardial initiator regions is boxed. A classic giardial polyadenylation signal sequence is underlined. (B) Alignment of the predicted BIVML protein (SEQ ID NO: 15) with the C-terminal region of human BIVM (SEQ ID NO: 2). Labeling is as in FIG. 3. (C) RNA blot (10 μg/track) probed for BIVML in vegetative-stage (veg) and 21 hr encysting Giardia. Calmodulin is shown as loading control.

[0018]FIG. 8. RT-PCR analysis of extracts from BIVM expressing and non-expressing human cell lines indicated that EP58/MGC5302 was expressed in all cell lines that express BIVM but not in a BIVM non-expressing cell line.

[0019]FIG. 9. Potential binding sites contained in the 41 bp region separating the BIVM and EP58/MGC5302 genes revealed sites for cell type specific factors such as the myeloid zinc finger-1 (MZF-1), the hematopoietic-expressed Ikaros-2 (IK2) factor, and the ubiquitously expressed transcription factors NF1, USF, NFκB, and NMYC.

[0020]FIG. 10. Detection of bands representing NFκB-specific binding constitutively present in nuclear extracts.

[0021]FIG. 11. Binding of the 41 bp intergenic region by NFκB complexes containing c-Rel and RelB factors, which are constitutively present in the nuclear extracts from the BIVM expressing K562 cell line.

[0022]FIG. 12. TNF-α activated NFκB increases the expression of BIVM in the BIVM-expressing HeLa cell line (DNS). A cell line devoid of basal BIVM expression, the Raji Burkitt's lymphoma line, is induced to express BIVM by TNF-a.

[0023]FIGS. 13A-13C. Flow cytometer analyses of cells stained with propidium iodide.

BRIEF DESCRIPTION OF THE SEQUENCES

[0024]SEQ ID NO: 1--human BIVM cDNA

[0025]SEQ ID NO: 2--human BIVM amino acid sequence

[0026]SEQ ID NO: 3--human BIVM gemonic sequence with upstream partial sequence of MGC5302 gene and downstream partial sequence of ERCC5 gene

[0027]SEQ ID NO: 4--Xenopus BIVM open reading frame

[0028]SEQ ID NO: 5--Xenopus BIVM amino acid sequence

[0029]SEQ ID NO: 6--Chicken BIVM open reading frame

[0030]SEQ ID NO: 7--Alternatively spliced chicken BIVM open reading frame

[0031]SEQ ID NO: 8--Chicken BIVM amino acid sequence

[0032]SEQ ID NO: 9--Alternatively splice chicken BIVM amino acid sequence

[0033]SEQ ID NO: 10--Zebrafish BIVM open reading frame

[0034]SEQ ID NO: 11--Zebrafish BIVM amino acid sequence

[0035]SEQ ID NO: 12--Sea urchin BIVM partial coding sequence

[0036]SEQ ID NO: 13--Sea urchin BIVM partial amino acid sequence

[0037]SEQ ID NO: 14--Giardia BIVM-like open reading frame

[0038]SEQ ID NO: 15--Giardia BIVM-like amino acid sequence

[0039]SEQ ID NO: 16--Lancelet BIVM partial coding sequence

[0040]SEQ ID NO: 17--Lancelet BIVM partial amino acid sequence

[0041]SEQ ID NO: 18--Mouse BIVM exon A nucleotide sequence

[0042]SEQ ID NO: 19--Mouse BIVM exon B nucleotide sequence

[0043]SEQ ID NO: 20--Mouse BIVM exon C nucleotide sequence

[0044]SEQ ID NO: 21--Mouse BIVM exon 1 nucleotide sequence

[0045]SEQ ID NO: 22--Alternative mouse BIVM 5' end clone (6359)

[0046]SEQ ID NO: 23--Alternative mouse BIVM 5' end clone (6358)

[0047]SEQ ID NO: 24--Alternative mouse BIVM 5' end clone (6356)

[0048]SEQ ID NO: 25--Alternative mouse BIVM 5' end clone (cDNA)

[0049]SEQ ID NO: 26--Mouse BIVM cDNA with clone 6359 5' end

[0050]SEQ ID NO: 27--Mouse BIVM amino acid sequence

[0051]SEQ ID NO: 28--Mouse BIVM genomic sequence with upstream partial sequence of KDEL gene

[0052]SEQ ID NO: 29--Human BIVM exon A¹ splice donor sequence

[0053]SEQ ID NO: 30--Human BIVM exon A² splice donor sequence

[0054]SEQ ID NO: 31--Human BIVM exon A³ splice donor sequence

[0055]SEQ ID NO: 32--Human BIVM exon B splice acceptor sequence

[0056]SEQ ID NO: 33--Human BIVM exon B splice donor sequence

[0057]SEQ ID NO: 34--Human BIVM exon 1 splice acceptor sequence

[0058]SEQ ID NO: 35--Human BIVM exon 1 splice donor sequence

[0059]SEQ ID NO: 36--Human BIVM exon 2 splice acceptor sequence

[0060]SEQ ID NO: 37--Human BIVM exon 2 splice donor sequence

[0061]SEQ ID NO: 38--Human BIVM exon 3 splice acceptor sequence

[0062]SEQ ID NO: 39--Human BIVM exon 3 splice donor sequence

[0063]SEQ ID NO: 40--Human BIVM exon 4 splice acceptor sequence

[0064]SEQ ID NO: 41--Human BIVM exon 4 splice donor sequence

[0065]SEQ ID NO: 42--Human BIVM exon 5 splice acceptor sequence

[0066]SEQ ID NO: 43--Human BIVM exon 5 splice donor sequence

[0067]SEQ ID NO: 44--Human BIVM exon 6 splice acceptor sequence

[0068]SEQ ID NO: 45--Human BIVM exon 6 splice donor sequence

[0069]SEQ ID NO: 46--Human BIVM exon 7 splice acceptor sequence

[0070]SEQ ID NO: 47--Human BIVM exon 7 splice donor sequence

[0071]SEQ ID NO: 48--Human BIVM exon 8 splice acceptor sequence

[0072]SEQ ID NO: 49--Human BIVM exon 8 splice donor sequence

[0073]SEQ ID NO: 50--Human BIVM exon 9 splice acceptor sequence

[0074]SEQ ID NO: 51--HSMAP5 primer

[0075]SEQ ID NO: 52--HSMAP6 primer

[0076]SEQ ID NO: 53--xfbivmMAPF1 primer

[0077]SEQ ID NO: 54--xfbivmMAPR1 primer

[0078]SEQ ID NO: 55--M1 amino acid motif

[0079]SEQ ID NO: 56--M2 amino acid motif

[0080]SEQ ID NO: 57--M3a amino acid motif

[0081]SEQ ID NO: 58--M3b amino acid motif

[0082]SEQ ID NO: 59--BIVM N-terminus region of homology

[0083]SEQ ID NO: 60--BIVM C-terminus region of homology

[0084]SEQ ID NO: 61--BIVM amino acid motif 1

[0085]SEQ ID NO: 62--BIVM amino acid motif 2

[0086]SEQ ID NO: 63--BIVM amino acid motif 3

[0087]SEQ ID NO: 64--BIVM amino acid motif 4

[0088]SEQ ID NO: 65--BIVM peptide domain (N-terminus of region of interspecific homology)

DETAILED DISCLOSURE OF THE INVENTION

[0089]The subject invention provides isolated and/or purified nucleotide sequences comprising: a) a polynucleotide sequence, or fragment thereof, or a polynucleotide encoding an amino acid sequence, or fragment of said amino acid sequence, of a sequence selected from the group consisting of SEQ ID NOs: 1-64 (or the complements of said polynucleotide sequences or fragments thereof); b) a polynucleotide sequence, or fragment thereof, comprising a sequence having at least about 20% to 99.99% identity to a polynucleotide selected from the group consisting of SEQ ID NOs: 1-28; c) a polynucleotide sequence encoding a polypeptide comprising a sequence selected from the group consisting of SEQ ID NOs: 2, 5, 7, 8, 9, 11, 13, 15, 17, or 27; d) splice variants of SEQ ID NOs: 1-3 or 6-9; or e) a polynucleotide sequence encoding a polypeptide fragment of SEQ ID NOs: 2, 5, 7, 8, 9, 11, 13, 15, 17, or 27, wherein said fragment has substantially the same biological or serologic activity as the native (or intact) polypeptide.

[0090]Nucleotide, polynucleotide, or nucleic acid sequence(s) are understood to mean. according to the present invention, either a double-stranded DNA, a single-stranded DNA, or products of transcription of the said DNAs (e.g., RNA molecules). It should also be understood that the present invention does not relate to the genomic nucleotide sequences encoding BIVM in their natural/native environment or natural/native state. The nucleic acid, polynucleotide, or nucleotide sequences of the invention have been isolated, purified (or partially purified), by separation methods including, but not limited to, ion-exchange chromatography, molecular size exclusion chromatography, affinity chromatography, or by genetic engineering methods such as amplification, cloning, or subcloning.

[0091]Optionally, the polynucleotide sequences of the instant invention can also contain one or more polynucleotides encoding heterologous polypeptide sequences (e.g., tags that facilitate purification of the polypeptides of the invention (see, for example, U.S. Pat. No. 6,342,362, hereby incorporated by reference in its entirety; Altendorf et al. [1999-WWW, 2000] "Structure and Function of the F_o Complex of the ATP Synthase from Escherichia Coli," J. of Experimental Biology 203:19-28, The Co. of Biologists, Ltd., G. B.; Baneyx [1999] "Recombinant Protein Expression in Escherichia coli," Biotechnology 10:411-21, Elsevier Science Ltd.; Eihauer et al. [2001] "The FLAG® Peptide, a Versatile Fusion Tag for the Purification of Recombinant Proteins," J. Biochem Biophys Methods 49:455-65; Jones et al. [1995] J. Chromatography 707:3-22; Jones et al. [1995] "Current Trends in Molecular Recognition and Bioseparation," J. of Chromatography A. 707:3-22, Elsevier Science B. V.; Margolin [2000] "Green Fluorescent Protein as a Reporter for Macromolecular Localization in Bacterial Cells," Methods 20:62-72, Academic Press; Puig et al. [2001] "The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification," Methods 24:218-29, Academic Press; Sassenfeld [1990] "Engineering Proteins for Purification," TibTech 8:88-93; Sheibani [1999] "Prokaryotic Gene Fusion Expression Systems and Their Use in Structural and Functional Studies of Proteins," Prep. Biochem. & Biotechnol. 29(1):77-90, Marcel Dekker, Inc.; Skerra et al. [1999] "Applications of a Peptide Ligand for Streptavidin: the Strep-tag", Biomolecular Engineering 16:79-86, Elsevier Science, B. V.; Smith [1998] "Cookbook for Eukaryotic Protein Expression: Yeast, Insect, and Plant Expression Systems," The Scientist 12(22):20; Smyth et al. [2000] "Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag", Methods in Molecular Biology, 139:49-57; Unger [1997] "Show Me the Money: Prokaryotic Expression Vectors and Purification Systems," The Scientist 11(17):20, each of which is hereby incorporated by reference in their entireties), or commercially available tags from vendors such as such as STRATAGENE (La Jolla, Calif.), NOVAGEN (Madison, Wis.), QIAGEN, Inc., (Valencia, Calif.), or InVitrogen (San Diego, Calif.).

TABLE-US-00001 TABLE I Splice variants of BIVM (SEQ ID NOs: 29-50) Seq ID Intron No. Exon Splice Donor Splice Acceptor Position (bp) 29 A¹ CGGCCCCAGGgtaac -- 1-415 -- 30 A² TGTGATCCAGgtccg -- 1-365 -- 31 A³ CAGGCCAGAGgtacc -- 1-473 -- 33/32 B TTTCTGTCAGgtgat ttccctaaagGAATC 474-557 5785 35/34 1 CACAAATCAGgtaag ttcctcttagGAGCT 558-1157 1754 37/36 2 TCAGACGATGgtgat tgtattctagGCAAT 1158-1284 8682 39/38 3 GAGCTGCAAAgtaag gtgttctcagGTACT 1285-1380 4481 41/40 4 CACTTATGAGgtatg tcttttgtagCCTTC 1381-1485 609 43/42 5 GGAGAAACTGgtagg ttactttcagGTGGT 1486-1580 216 45/44 6 AAGCATTCAGgtaag tttttaatagCTTCA 1581-1713 9405 49/48 7 AACAAAGAAGgtaag ttaactatagATGGG 1714-1800 2768 50 8 -- ttcttctcagGTTGG 1801-1897 4089 50 9 -- ttcttctcagGTTGG 1898-3029 832

[0092]Other aspects of the invention provide vectors containing one or more of the polynucleotides of the invention. The vectors can be vaccine, replication, or amplification vectors. In some embodiments of this aspect of the invention, the polynucleotides are operably associated with regulatory elements capable of causing the expression of the polynucleotide sequences. Such vectors include, among others, chromosomal, episomal and virus-derived vectors, e.g., vectors derived from bacterial plasmids, from bacteriophage, from transposons, from yeast episomes, from insertion elements, from yeast chromosomal elements, from viruses such as baculoviruses, papova viruses, such as SV40, vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations of the aforementioned vector sources, such as those derived from plasmid and bacteriophage genetic elements (e.g., cosmids and phagemids).

[0093]As indicated above, vectors of this invention can also comprise elements necessary to provide for the expression and/or the secretion of a polypeptide encoded by the nucleotide sequences of the invention in a given host cell. The vector can contain one or more elements selected from the group consisting of a promoter, signals for initiation of translation, signals for termination of translation, and appropriate regions for regulation of transcription. In certain embodiments, the vectors can be stably maintained in the host cell and can, optionally, contain signal sequences directing the secretion of translated protein. Other embodiments provide vectors that are not stable in transformed host cells. Vectors can integrate into the host genome or be autonomously-replicating vectors.

[0094]In a specific embodiment, a vector comprises a promoter operably linked to a protein or peptide-encoding nucleic acid sequence, one or more origins of replication, and, optionally, one or more selectable markers (e.g., an antibiotic resistance gene). Non-limiting exemplary vectors for the expression of the polypeptides of the invention include pBr-type vectors, pET-type plasmid vectors (Promega), pBAD plasmid vectors (Invitrogen) or those provided in the examples below. Furthermore, vectors according to the invention are useful for transforming host cells for the cloning or expression of the nucleotide sequences of the invention.

[0095]Promoters which may be used to control expression include, but are not limited to, the CMV promoter, the SV40 early promoter region (Bernoist and Chambon [1981] Nature 290:304-310), the promoter contained in the 3' long terminal repeat of Rous sarcoma virus (Yamamoto, et al. [1980] Cell 22:787-797), the herpes thymidine kinase promoter (Wagner et al. Proc. Natl. Acad. Sci. USA 78:1441-1445), the regulatory sequences of the metallothionein gene (Brinster et al. [1982] Nature 296:39-42); prokaryotic vectors containing promoters such as the β-lactamase promoter (Villa-Kamaroff, et al. [1978] Proc. Natl. Acad. Sci. USA 75:3727-3731), or the tac promoter (DeBoer, et al. [1983] Proc. Natl. Acad. Sci. USA 80:21-25); see also, "Useful Proteins from Recombinant Bacteria" in Scientific American, 1980, 242:74-94; plant expression vectors comprising the nopaline synthetase promoter region (Herrera-Estrella et al. [1983] Nature 303:209-213) or the cauliflower mosaic virus 35S RNA promoter (Gardner, et al. [1981] Nucl. Acids Res. 9:2871), and the promoter of the photosynthetic enzyme ribulose biphosphate carboxylase (Herrera-Estrella et al. [1984] Nature 310:115-120); promoter elements from yeast or fungi such as the Gal 4 promoter, the ADC (alcohol dehydrogenase) promoter, PGK (phosphoglycerol kinase) promoter, and/or the alkaline phosphatase promoter.

[0096]The subject invention also provides for "homologous" or "modified" nucleotide sequences. Modified nucleic acid sequences will be understood to mean any nucleotide sequence obtained by mutagenesis according to techniques well known to persons skilled in the art, and exhibiting modifications in relation to the normal sequences. For example, mutations in the regulatory and/or promoter sequences for the expression of a polypeptide that result in a modification of the level of expression of a polypeptide according to the invention provide for a "modified nucleotide sequence". Likewise, substitutions, deletions, or additions of nucleic acid to the polynucleotides of the invention provide for "homologous" or "modified" nucleotide sequences. In various embodiments, "homologous" or "modified" nucleic acid sequences have substantially the same biological or serological activity as the native (naturally occurring) BIVM polypeptides. A "homologous" or "modified" nucleotide sequence will also be understood to mean a splice variant of the polynucleotides of the instant invention (see Table I) or any nucleotide sequence encoding a "modified polypeptide" as defined below.

[0097]A homologous nucleotide sequence, for the purposes of the present invention, encompasses a nucleotide sequence having a percentage identity with the bases of the nucleotide sequences of between at least (or at least about) 20.00% to 99.99% or higher. The aforementioned range of percent identity is to be taken as including, and providing written description and support for, any fractional percentage, in intervals of 0.01%, between 20.00% and 99.99% or higher. These percentages are purely statistical and differences between two nucleic acid sequences can be distributed randomly and over the entire sequence length.

[0098]In various embodiments, homologous sequences exhibiting a percentage identity with the bases of the nucleotide sequences of the present invention can have 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99 percent identity with the polynucleotide sequences of the instant invention.

[0099]Both protein and nucleic acid sequence homologies may be evaluated using any of the variety of sequence comparison algorithms and programs known in the art. Such algorithms and programs include, but are by no means limited to, TBLASTN, BLASTP, FASTA, TFASTA, and CLUSTALW (Pearson and Lipman [1988] Proc. Natl. Acad. Sci. USA 85(8):2444-2448; Altschul et al. [1990] J. Mol. Biol. 215(3):403-410; Thompson et al. [1994] Nucleic Acids Res. 22(2):4673-4680; Higgins et al. [1996] Methods Enzymol. 266:383-402; Altschul et al. [1990] J. Mol. Biol. 215(3):403-410; Altschul et al. [1993] Nature Genetics 3:266-272).

[0100]The subject invention also provides nucleotide sequences complementary to any of the polynucleotide sequences disclosed herein. Thus, the invention is understood to include any DNA whose nucleotides are complementary to those of the sequence of the invention, and whose orientation is reversed (e.g., an antisense sequence).

[0101]The present invention further provides fragments of the polynucleotide sequences provided herein. Representative fragments of the polynucleotide sequences according to the invention will be understood to mean any nucleotide fragment having at least 8 or 9 successive nucleotides, preferably at least 12 successive nucleotides, and still more preferably at least 15 or at least 20 successive nucleotides of the sequence from which it is derived. In other embodiments, fragments contain from one nucleotide less than the full length polynucleotide sequence to fragments comprising up to, and including 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, or 255 consecutive nucleotides of a particular sequence disclosed herein. Yet other embodiments provide fragments (or detection probes) comprising nucleotides 1446 to 1697 or 1447 to 1698 of FIG. 1 (SEQ ID NO:1). It is to be understood that such fragments refer only to portions of the disclosed polynucleotide sequences that are not listed in a publicly available database or prior art references.

[0102]Among these representative fragments, those capable of hybridizing under stringent conditions with a nucleotide sequence according to the invention are preferred. Conditions of high or intermediate stringency are provided infra and are chosen to allow for hybridization between two complementary DNA fragments. Hybridization conditions for a polynucleotide of about 300 bases in size will be adapted by persons skilled in the art for larger- or smaller-sized oligonucleotides, according to methods well known in the art (see, for example, Sambrook et al. [1989]).

[0103]The subject invention also provides detection probes (e.g., fragments of the disclosed polynucleotide sequences) for hybridization with a target sequence or an amplicon generated from the target sequence. Such a detection probe will advantageously have as sequence a sequence of at least 9, 12, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or 100 nucleotides. Alternatively, detection probes can comprise 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, or 255 consecutive nucleotides of the disclosed nucleic acids. The detection probes can also be used as labeled probe or primer in the subject invention. Labeled probes or primers are labeled with a radioactive compound or with another type of label. Alternatively, non-labeled nucleotide sequences may be used directly as probes or primers; however, the sequences are generally labeled with a radioactive element (³²P, ³5S, ³H, ¹25I) or with a molecule such as biotin, acetylaminofluorene, digoxigenin, 5-bromo-deoxyuridine, or fluorescein to provide probes that can be used in numerous applications.

[0104]The nucleotide sequences according to the invention may also be used in analytical systems, such as DNA chips. DNA chips and their uses are well known in the art and (see for example, U.S. Pat. Nos. 5,561,071; 5,753,439; 6,214,545; Schena et al. [1996] BioEssays 18:427-431; Bianchi et al. [1997] Clin. Diagn. Virol. 8:199-208; each of which is hereby incorporated by reference in their entireties) and/or are provided by commercial vendors such as Affymetrix, Inc. (Santa Clara, Calif.).

[0105]Various degrees of stringency of hybridization can be employed. The more severe the conditions, the greater the complementarity that is required for duplex formation. Severity of conditions can be controlled by temperature, probe concentration, probe length, ionic strength, time, and the like. Preferably, hybridization is conducted under moderate to high stringency conditions by techniques well known in the art, as described, for example, in Keller, G. H., M. M. Manak [1987] DNA Probes, Stockton Press, New York, N.Y., pp. 169-170.

[0106]By way of example, hybridization of immobilized DNA on Southern blots with ³²P-labeled gene-specific probes can be performed by standard methods (Maniatis et al. [1982] Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York). In general, hybridization and subsequent washes can be carried out under moderate to high stringency conditions that allow for detection of target sequences with homology to the exemplified polynucleotide sequence. For double-stranded DNA gene probes, hybridization can be carried out overnight at 20-25° C. below the melting temperature (Tm) of the DNA hybrid in 6×SSPE, 5× Denhardt's solution, 0.1% SDS, 0.1 mg/ml denatured DNA. The melting temperature is described by the following formula (Beltz et al. [1983] Methods of Enzymology, R. Wu, L. Grossman and K. Moldave [eds.] Academic Press, New York 100:266-285).

[0107]T_m=81.5° C.+16.6 Log[Na+]+0.41(% G+C)-0.61(% formamide)-600/length of duplex in base pairs.

[0108]Washes are typically carried out as follows:

(1) twice at room temperature for 15 minutes in 1×SSPE, 0.1% SDS (low stringency wash);(2) once at T_m-20° C. for 15 minutes in 0.2×SSPE, 0.1% SDS (moderate stringency wash).

[0109]For oligonucleotide probes, hybridization can be carried out overnight at 10-20° C. below the melting temperature (T_m) of the hybrid in 6×SSPE, 5× Denhardt's solution, 0.1% SDS, 0.1 mg/ml denatured DNA. T_m for oligonucleotide probes can be determined by the following formula:

[0110]T_m(° C.)=2(number T/A base pairs)+4(number G/C base pairs) (Suggs et al. [1981] ICN-UCLA Symp. Dev. Biol. Using Purified Genes, D. D. Brown [ed.], Academic Press, New York, 23:683-693).

[0111]Washes can be carried out as follows:

(1) twice at room temperature for 15 minutes 1×SSPE, 0.1% SDS (low stringency wash;2) once at the hybridization temperature for 15 minutes in 1×SSPE, 0.1% SDS (moderate stringency wash).

[0112]In general, salt and/or temperature can be altered to change stringency. With a labeled DNA fragment >70 or so bases in length, the following conditions can be used: [0113]Low: 1 or 2×SSPE, room temperature [0114]Low: 1 or 2×SSPE, 42° C. [0115]Moderate: 0.2× or 1×SSPE, 65° C. [0116]High: 0.1×SSPE, 65° C.

[0117]By way of another non-limiting example, procedures using conditions of high stringency can also be performed as follows: Pre-hybridization of filters containing DNA is carried out for 8 h to overnight at 65° C. in buffer composed of 6×SSC, 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA, and 500 μg/ml denatured salmon sperm DNA. Filters are hybridized for 48 h at 65° C., the preferred hybridization temperature, in pre-hybridization mixture containing 100 μg/ml denatured salmon sperm DNA and 5-20×10⁶ cpm of ³²P-labeled probe. Alternatively, the hybridization step can be performed at 65° C. in the presence of SSC buffer, 1×SSC corresponding to 0.15M NaCl and 0.05 M Na citrate. Subsequently, filter washes can be done at 37° C. for 1 h in a solution containing 2×SSC, 0.01% PVP, 0.01% Ficoll, and 0.01% BSA, followed by a wash in 0.1×SSC at 50° C. for 45 min. Alternatively, filter washes can be performed in a solution containing 2×SSC and 0.1% SDS, or 0.5×SSC and 0.1% SDS, or 0.1×SSC and 0.1% SDS at 68° C. for 15 minute intervals. Following the wash steps, the hybridized probes are detectable by autoradiography. Other conditions of high stringency which may be used are well known in the art (see, for example, Sambrook et al. [1989] Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor Press, N.Y., pp. 9.47-9.57; and Ausubel et al. [1989] Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, New York, each incorporated herein in its entirety).

[0118]A further non-limiting example of procedures using conditions of intermediate stringency are as follows: Filters containing DNA are pre-hybridized, and then hybridized at a temperature of 60° C. in the presence of a 5×SSC buffer and labeled probe. Subsequently, filters washes are performed in a solution containing 2×SSC at 50° C. and the hybridized probes are detectable by autoradiography. Other conditions of intermediate stringency which may be used are well known in the art (see, for example, Sambrook et al. [1989] Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor Press, N.Y., pp. 9.47-9.57; and Ausubel et al. [1989] Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, New York, each of which is incorporated herein in its entirety).

[0119]Duplex formation and stability depend on substantial complementarity between the two strands of a hybrid and, as noted above, a certain degree of mismatch can be tolerated. Therefore, the probe sequences of the subject invention include mutations (both single and multiple), deletions, insertions of the described sequences, and combinations thereof, wherein said mutations, insertions and deletions permit formation of stable hybrids with the target polynucleotide of interest. Mutations, insertions and deletions can be produced in a given polynucleotide sequence in many ways, and these methods are known to an ordinarily skilled artisan. Other methods may become known in the future.

[0120]It is also well known in the art that restriction enzymes can be used to obtain functional fragments of the subject DNA sequences. For example, Bal31 exonuclease can be conveniently used for time-controlled limited digestion of DNA (commonly referred to as "erase-a-base" procedures). See, for example, Maniatis et al. [1982] Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York; Wei et al. [1983] J. Biol. Chem. 258:13006-13512. The nucleic acid sequences of the subject invention can also be used as molecular weight markers in nucleic acid analysis procedures.

[0121]The invention also provides host cells transformed by a polynucleotide according to the invention and the production of BIVM (or BIVM ortholog) polypeptides by the transformed host cells. In some embodiments, transformed cells comprise an expression vector containing BIVM, or BIVM ortholog, polynucleotide sequences. Other embodiments provide for host cells transformed with nucleic acids. Yet other embodiments provide transformed cells comprising an expression vector containing fragments of BIVM, or BIVM ortholog, polynucleotide sequences. Transformed host cells according to the invention are cultured under conditions allowing the replication and/or the expression of the nucleotide sequences of the invention. Expressed polypeptides are recovered from culture media and purified, for further use, according to methods known in the art.

[0122]The host cell may be chosen from eukaryotic or prokaryotic systems, for example bacterial cells (Gram negative or Gram positive), yeast cells, animal cells, plant cells, and/or insect cells using baculovirus vectors. In some embodiments, the host cell for expression of the polypeptides include, and are not limited to, those taught in U.S. Pat. Nos. 6,319,691; 6,277,375; 5,643,570; 5,565,335; Unger [1997] The Scientist 11(17):20; or Smith [1998] The Scientist 12(22):20, each of which is incorporated by reference in its entirety, including all references cited within each respective patent or reference. Other exemplary, and non-limiting, host cells include Staphylococcus spp., Enterococcus spp., E. coli, and Bacillus subtilis; fungal cells, such as Streptomyces spp., Aspergillus spp., S. cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, Hansela polymorpha, Kluveromyces lactis, and Yarrowia lipolytica; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as CHO, COS, HeLa, C127, 3T3, BHK, 293 and Bowes melanoma cells; and plant cells. A great variety of expression systems can be used to produce the polypeptides of the invention and polynucleotides can be modified according to methods known in the art to provide optimal codon usage for expression in a particular expression system.

[0123]Furthermore, a host cell strain may be chosen that modulates the expression of the inserted sequences, modifies the gene product, and/or processes the gene product in the specific fashion. Expression from certain promoters can be elevated in the presence of certain inducers; thus, expression of the genetically engineered polypeptide may be controlled. Furthermore, different host cells have characteristic and specific mechanisms for the translational and post-translational processing and modification (e.g., glycosylation, phosphorylation) of proteins. Appropriate cell lines or host systems can be chosen to ensure the desired modification and processing of the foreign protein expressed. For example, expression in a bacterial system can be used to produce an unglycosylated core protein product whereas expression in yeast will produce a glycosylated product. Expression in mammalian cells can be used to provide "native" glycosylation of a heterologous protein. Furthermore, different vector/host expression systems may effect processing reactions to different extents.

[0124]Nucleic acids and/or vectors can be introduced into host cells by well-known methods, such as, calcium phosphate transfection, DEAE-dextran mediated transfection, transfection, microinjection, cationic lipid-mediated transfection, electroporation, transduction, scrape loading, ballistic introduction and infection (see, for example, Sambrook et al. [1989] Molecular Cloning: A Laboratory Manual, 2^nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).

[0125]The subject invention also provides for the expression of a polypeptide, derivative, or a variant (e.g., a splice variant) encoded by a polynucleotide sequence disclosed herein. Alternatively, the invention provides for the expression of a polypeptide fragment obtained from a polypeptide, derivative, or a variant encoded by a polynucleotide fragment derived from the polynucleotide sequences disclosed herein. In either embodiment, the disclosed sequences can be regulated by a second nucleic acid sequence so that the polypeptide or fragment is expressed in a host transformed with a recombinant DNA molecule according to the subject invention. For example, expression of a protein or peptide may be controlled by any promoter/enhancer element known in the art.

[0126]The subject invention also provides nucleic acid based methods for the identification of the presence of the BIVM gene, or orthologs thereof, in a sample. These methods can utilize the nucleic acids of the subject invention and are well known to those skilled in the art (see, for example, Sambrook et al. [1989] or Abbaszadega [2001] "Advanced Detection of Viruses and Protozoan Parasites in Water," Reviews in Biology and Biotechnology, 1(2):21-26). Among the techniques useful in such methods are enzymatic gene amplification (or PCR), Southern blots, Northern blots, or other techniques utilizing nucleic acid hybridization for the identification of polynucleotide sequences in a sample. Thus, the subject invention can provide nucleic acid based methodologies for the identification of G. lamblia in environmental or biological samples and provides sensitive assays for the diagnosis of G. lamblia infections. Alternatively, the nucleic acids can be used to screen individuals for cancers, tumors, or malignancies associated with dysregulation of the BIVM gene or its transcriptional products.

[0127]The subject invention also provides polypeptides encoded by nucleotide sequences of the invention. The subject invention also provides fragments of at least 5 amino acids of a polypeptide encoded by the polynucleotides of the instant invention. In some embodiments, the polypeptide fragments are reactive with antibodies found in the serum of an individual infected with G. lamblia.

[0128]In the context of the instant invention, the terms polypeptide, peptide and protein are used interchangeably. Likewise, the terms variant and homologous are also used interchangeably. It should be understood that the invention does not relate to the polypeptides in natural form or native environment. Peptides and polypeptides according to the invention have been isolated or obtained by purification from natural sources (or their native environment), chemically synthesized, or obtained from host cells prepared by genetic manipulation (e.g., the polypeptides, or fragments thereof, are recombinantly produced by host cells). Polypeptides according to the instant invention may also contain non-natural amino acids, as will be described below.

[0129]"Variant" or "homologous" polypeptides will be understood to designate the polypeptides containing, in relation to the native polypeptide, modifications such as deletion, addition, or substitution of at least one amino acid, truncation, extension, or the addition of chimeric heterologous polypeptides. Optionally, "variant" or "homologous" polypeptides can contain a mutation or post-translational modifications. Among the "variant" or "homologous" polypeptides, those whose amino acid sequence exhibits 20.00% to 99.99% (inclusive) identity to the native polypeptide sequence are preferred. The aforementioned range of percent identity is to be taken as including, and providing written description and support for, any fractional percentage, in intervals of 0.01%, between 50.00% and, up to, including 99.99%. These percentages are purely statistical and differences between two polypeptide sequences can be distributed randomly and over the entire sequence length.

[0130]"Variant" or "homologous" polypeptide sequences exhibiting a percentage identity with the polypeptides of the present invention can, alternatively, have 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 91, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99 percent identity with the polypeptide sequences of the instant invention. The expression equivalent amino acid is intended here to designate any amino acid capable of being substituted for one of the amino acids in the basic structure without, however, essentially modifying the biological activities of the corresponding peptides and as provided below.

[0131]By way of example, amino acid substitutions can be carried out without resulting in a substantial modification of the biological activity of the corresponding modified polypeptides; for example, the replacement of leucine with valine or isoleucine; aspartic acid with glutamic acid; glutamine with asparagine; arginine with lysine; and the reverse substitutions can be performed without substantial modification of the biological activity of the polypeptides.

[0132]In other embodiments, homologous polypeptides according to the subject invention also include various splice variants identified within the BIVM coding sequence (see Table I).

[0133]The subject invention also provides biologically active fragments of a polypeptide according to the invention and includes those peptides capable of eliciting an immune response. In one embodiment, an immune response directed against G. lamblia is provided. The immune response can provide components (either antibodies or components of the cellular immune response (e.g., B-cells, helper, cytotoxic, and/or suppressor T-cells)) reactive with the biologically active fragment of a polypeptide, the intact, full length, unmodified polypeptide disclosed herein, or both the biologically active fragment of a polypeptide and the intact, full length, unmodified polypeptides disclosed herein. Biologically active fragments according to the invention comprise from five (5) amino acids to one amino acid less than the full length of any polypeptide sequence provided herein. Alternatively, fragments comprising 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 205, 210, 215, 220, 225, 230, 235, 240, 245, 250, or 255 consecutive amino acids of a disclosed polypeptide sequence are provided herein.

[0134]Fragments, as described herein, can be obtained by cleaving the polypeptides of the invention with a proteolytic enzyme (such as trypsin, chymotrypsin, or collagenase) or with a chemical reagent, such as cyanogen bromide (CNBr). Alternatively, polypeptide fragments can be generated in a highly acidic environment, for example at pH 2.5. Such polypeptide fragments may be equally well prepared by chemical synthesis or using hosts transformed with an expression vector containing nucleic acids encoding polypeptide fragments according to the invention. The transformed host cells contain a nucleic acid and are cultured according to well-known methods; thus, the invention allows for the expression of these fragments, under the control of appropriate elements for regulation and/or expression of the polypeptide fragments.

[0135]Modified polypeptides according to the invention are understood to designate a polypeptide obtained by variation in the splicing of transcriptional products of the BIVM gene, genetic recombination, or by chemical synthesis as described below. Modified polypeptides contain at least one modification in relation to the normal polypeptide sequence. These modifications can include the addition, substitution, deletion of amino acids contained within the polypeptides of the invention.

[0136]In order to extend the life of the polypeptides of the invention, it may be advantageous to use non-natural amino acids, for example in the D form, or alternatively amino acid analogs, such as sulfur-containing forms of amino acids. Alternative means for increasing the life of polypeptides can also be used in the practice of the instant invention. For example, polypeptides of the invention, and fragments thereof, can be recombinantly modified to include elements that increase the plasma, or serum half-life of the polypeptides of the invention. These elements include, and are not limited to, antibody constant regions (see for example, U.S. Pat. No. 5,565,335, hereby incorporated by reference in its entirety, including all references cited therein), or other elements such as those disclosed in U.S. Pat. Nos. 6,319,691; 6,277,375; or 5,643,570, each of which is incorporated by reference in its entirety, including all references cited within each respective patent. Alternatively, the polynucleotides and genes of the instant invention can be recombinantly fused to elements that are useful in the preparation of immunogenic constructs for the purposes of vaccine formulation or elements useful for the isolation of the polypeptides of the invention.

[0137]The polypeptides, fragments, and immunogenic fragments of the invention may further contain linkers that facilitate the attachment of the fragments to a carrier molecule for the stimulation of an immune response or diagnostic purposes. The linkers can also be used to attach fragments according to the invention to solid support matrices for use in affinity purification protocols. In this aspect of the invention, the linkers specifically exclude, and are not to be considered anticipated, where the fragment is a subsequence of another peptide, polypeptide, or protein as identified in a search of protein sequence databases as indicated in the preceding paragraph. In other words, the non-identical portions of the other peptide, polypeptide, of protein is not considered to be a "linker" in this aspect of the invention. Non-limiting examples of "linkers" suitable for the practice of the invention include chemical linkers (such as those sold by Pierce, Rockford, Ill.), peptides which allow for the connection of the immunogenic fragment to a carrier molecule (see, for example, linkers disclosed in U.S. Pat. Nos. 6,121,424; 5,843,464; 5,750,352; and 5,990,275, hereby incorporated by reference in their entirety). In various embodiments, the linkers can be up to 50 amino acids in length, up to 40 amino acids in length, up to 30 amino acids in length, up to 20 amino acids in length, up to 10 amino acids in length, or up to 5 amino acids in length.

[0138]In other specific embodiments, the polypeptides, peptides, derivatives, or analogs thereof may be expressed as a fusion, or chimeric protein product (comprising the protein, fragment, analog, or derivative joined via a peptide bond to a heterologous protein sequence (e.g., a different protein)). Such a chimeric product can be made by ligating the appropriate nucleic acid sequences encoding the desired amino acid sequences to each other by methods known in the art, in the proper coding frame, and expressing the chimeric product by methods commonly known in the art (see, for example, U.S. Pat. No. 6,342,362, hereby incorporated by reference in its entirety; Altendorf et al. [1999-WWW, 2000] "Structure and Function of the F_o Complex of the ATP Synthase from Escherichia Coli," J. of Experimental Biology 203:19-28, The Co. of Biologists, Ltd., G. B.; Baneyx [1999] "Recombinant Protein Expression in Escherichia coli," Biotechnology 10:411-21, Elsevier Science Ltd.; Eihauer et al.

[0139]"The FLAG® Peptide, a Versatile Fusion Tag for the Purification of Recombinant Proteins," J. Biochem Biophys Methods 49:455-65; Jones et al. [1995] J. Chromatography 707:3-22; Jones et al. [1995] "Current Trends in Molecular Recognition and Bioseparation," J. Chromatography A. 707:3-22, Elsevier Science B. V.; Margolin [2000] "Green Fluorescent Protein as a Reporter for Macromolecular Localization in Bacterial Cells," Methods 20:62-72, Academic Press; Puig et al. [2001] "The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification," Methods 24:218-29, Academic Press; Sassenfeld [1990] "Engineering Proteins for Purification," TibTech 8:88-93; Sheibani [1999] "Prokaryotic Gene Fusion Expression Systems and Their Use in Structural and Functional Studies of Proteins," Prep. Biochem. & Biotechnol. 29(1):77-90, Marcel Dekker, Inc.; Skerra et al. [1999] "Applications of a Peptide Ligand for Streptavidin: The Strep-tag", Biomolecular Engineering 16:79-86, Elsevier Science, B. V.; Smith [1998] "Cookbook for Eukaryotic Protein Expression: Yeast, Insect, and Plant Expression Systems," The Scientist 12(22):20; Smyth et al. [2000] "Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag", Methods in Molecular Biology, 139:49-57; Unger [1997] "Show Me the Money: Prokaryotic Expression Vectors and Purification Systems," The Scientist 11(17):20, each of which is hereby incorporated by reference in their entireties). Alternatively, such a chimeric product may be made by protein synthetic techniques, e.g., by use of a peptide synthesizer.

[0140]Another embodiment of the subject invention provides for the use of polypeptides encoded by the polynucleotides of the subject invention for the induction of an immune response or protective immunity in a subject to which the polypeptides are administered. In this aspect of the invention, compositions containing polypeptide are administered to a subject in amounts sufficient to induce an immune response, and/or induce protective immunity. The composition administered to the subject may, optionally, contain an adjuvant and may be delivered to the subject in any manner known in the art for the delivery of immunogen to a subject. Compositions may be formulated in any carriers, including for example, carriers described in E. W. Martin's Remington's Pharmaceutical Science, Mack Publishing Company, Easton, Pa.

[0141]The expression of the BIVM gene or BIVM gene product (e.g., DNA, RNA, or polypeptide) is dysregulated in a variety of cancers, tumors, and/or malignancies. Non-limiting examples of such cancers, tumors, and/or malignancies include prostate cancer, breast cancer, melanoma, chronic myelogenous leukemia, cervical cancer, adenocarcinomas, lymphoblastic leukemia, colorectal cancer, and lung carcinoma. Accordingly, the present invention provides a method for screening, or aiding in the diagnosis of, an individual suspected of having a malignancy or cancer. The subject invention provides methods comprising the steps of determining the amount of BIVM in a biological sample obtained from said individual and comparing the measured amount of BIVM to the amount of BIVM found in the normal population. The presence of a significantly increased amount of BIVM is associated with an indication of a malignancy or cancer. BIVM gene product can be detected by well-known methodologies including, and not limited to, Western blots, enzyme linked immunoassays (ELISAs), radioimmunoassays (RIAs), Northern blots, Southern blots, PCR-based assays, or other assays for the quantification of gene product known to the skilled artisan. This information, in conjunction with other information available to the skilled practitioner, assists in making a diagnosis.

[0142]The terms "comprising", "consisting of" and "consisting essentially of" are defined according to their standard meaning and may be substituted for one another throughout the instant application in order to attach the specific meaning associated with each term.

[0143]Following examples illustrate procedures for practicing the invention. These examples should not be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted.

EXAMPLE 1--IDENTIFICATION OF BIVM

[0144]Human BIVM was identified originally as an EST (IMAGE #785450; GenBank AA449273) that encodes the two short motifs WFRQ (motif 2 [M2]) and YFC (motif 3a [M3a]), which correspond to framework region 2 (FR2) and FR3 of an Ig V domain, respectively (Barclay, A. N., et al. [1997] The Leucocyte Antigen FactsBook, Academic Press, San Diego). The W in M2 and C in M3a correspond to W⁴¹ and C¹04 of the IMGT numbering system. Complete sequencing of this EST, overlapping ESTs (IMAGE #2184889, GenBank AI538125; IMAGE 136117, GenBank R33273; IMAGE 1060823, GenBank AA568610; and IMAGE 785450, GenBank AA449273) and RACE strategies were used to resolve the complete mRNA sequence.

[0145]Human BIVM mRNA is 3857 nucleotides and encodes a 503 amino acid protein (FIG. 1). No proteins with significant identities (E<0.01) to BIVM have been identified using BLAST analyses. Searches of current motif databases (BLOCKS, PRINTS, Conserved Domain Database, Domain Architecture Retrieval Tool, Simple Modular Architecture Research Tool) also failed to identify any additional significant motifs within the BIVM protein.

[0146]In addition to the shared M2 and M3a motifs, a second V domain FR3 motif, YHC (M3b), is located several residues amino terminal of M3a. Furthermore, a putative FR1 motif (M1), encoding the conserved V domain residues G¹⁶ and C²³ (IMGT amino acid numbering), was identified by visual inspection of BIVM peptide sequences (FIG. 1).

[0147]The 42 amino acids between M1 and M2 in BIVM are inconsistent with the sequence relationship in a V region in which the corresponding motifs would be separated by no more than 12 residues. This increased distance between C²³ and C¹04 of M1 and M3a (or M3b), which normally form a disulfide bridge and stabilize the Ig domain architecture, strongly suggests that BIVM is not a member of the IgSF. Since these peptide motifs are extremely short, it could be argued that their presence in BIVM may be a random occurrence. However, it should be emphasized that in the original search of the EST database, only 17 sequences were identified that encode W(Y/F)R(Q/H) and YFC that are correctly spaced and maintain an open reading frame. Of these 17 sequences, 16 were TCR cDNAs (encoding WYRQ) and one was BIVM (encoding WFRQ) (Hawke, N. A., et al. [1999] "Expanding Our Understanding of Immunoglobulin, T-cell Antigen Receptor, and Novel Immune-Type Receptor Genes: a Subset of the Immunoglobulin Gene Superfamily," Immunogenetics 50:124-133).

EXAMPLE 2--GENOMIC ORGANIZATION OF BIVM

[0148]GeneBridge 4 radiation hybrid panel mapping (Gyapay, G., et al. [1996] "A Radiation Hybrid Map of the Human Genome," Hum Mol Genet 5:339-346) localized BIVM on chromosome 13q32-33 (data not shown). Examination of the publicly available Human Genome Project database revealed the exon-intron structure of BIVM. A 5' truncated BIVM sequence (hypothetical protein FLJ20159) was initially placed on the publicly available human genome map at 13q14-q21. The 5' untranslated region of BIVM consists of two separate exons (designated exons A and B), followed by the coding region consisting of nine exons; the exon/intron boundaries are indicated in Table I.

[0149]Inspection of genomic sequence localizes BIVM between ERCC5 and "hypothetical protein" MGC5302, a human ortholog of the gene encoding the mouse protein Kdel1/EP58 (Kimata, Y., et al. [2000] "Identification of a Novel Mammalian Endoplasmicreticulum-Resident KDEL Protein Using an EST Database Motif Search," Gene 261:321-327). A CpG island is located in the 5' untranslated region of BIVM; the 3' untranslated region contains an Alu sequence (FIGS. 1 and 2). The Alu polyA sequence in the 3' untranslated region leads to the spurious production of 3' truncated cDNAs including many that are represented as ESTs.

[0150]Multiple 5' untranslated region splice variants were observed in analysis of 5' RACE products. Specifically, exon A has at least 3 splice donor sequences and exon B, which has a poor splice acceptor sequence, can be absent from the mature transcript (FIGS. 1 and 2; Table I). In addition, it is likely that multiple transcriptional start sites are present (FIG. 1).

EXAMPLE 3--BIVM IS HIGHLY CONSERVED WITHIN DEUTEROSTOME SPECIES

[0151]BIVM orthologs were identified in: mouse, chicken, Xenopus and zebrafish in order to address its potential phylogenetic conservation, as well as to define conserved motifs potentially relevant to function. In addition, a partial sequence for a BIVM ortholog was identified in sea urchin. The identity of the human BIVM protein to these orthologs ranges from 35-87% overall and is consistent with the phylogenetic relationships of the species considered (FIG. 3; see below). The C-terminal region of BIVM shares the highest degree of interspecific sequence identity. The N-terminus of this peptide domain is RK(V/C)LD (SEQ ID NO: 65) and the C-terminus is GGNLHC (SEQ ID NO: 60). This region includes all of the V domain motifs, and is 220 amino acids in human (indicated by arrowheads in FIG. 3).

[0152]The corresponding domains in mouse, chicken, Xenopus, zebrafish and sea urchin are 97%, 91%, 91%, 87% and 64% identical to the human domain, respectively. In addition, BIVM ESTs have been identified from an ascidian, sea squirt (Halocynthia roretzi) (e.g., GenBank AV385966), and a BIVM cDNA fragment has been isolated from a protochordate (cephalochordate), lancelet (Branchiostoma floridae), using an RT-PCR strategy (Yoder and Litman, GenBank AF411393). Their sequences within this domain are highly conserved.

EXAMPLE 4--CLOSE PHYSICAL LINKAGE OF BIVM AND EP58/MGC5302

[0153]Human BIVM maps between EP58/MGC5302 and ERCC5 on 13q. The human EP58 EST (that extends most 5'), places the transcriptional start sites of EP58 and BIVM only 41 bp apart. We identified a mouse BIVM genomic clone (from a e FixII library), which also encodes the 5' end of Ep58/Kdel1 (FIG. 4). The mapping position of Ep58/Kdel1 and BIVM in mouse has not yet been determined. The tight physical linkage of the EP58 to BIVM (41 bp in human and 224 bp in mouse) is consistent with a shared regulatory control system that functions in opposite directions (FIG. 4). Notably, both Ep58 and BIVM appear to be ubiquitously expressed (FIG. 5) (Kimata, Y., et al. [2000] "Identification of a Novel Mammalian Endoplasmicreticulum-Resident KDEL Protein Using an EST Database Motif Search," Gene 261:321-327). Finally, zebrafish BIVM has been mapped to linkage group 6 (LG6); however, its linkage relationship to kdel1 is unknown.

EXAMPLE 5--EXPRESSION OF HUMAN BIVM

[0154]The human BIVM transcript is ˜3.8 kb and appears to be expressed ubiquitously; the highest relative levels of expression are in spleen, ovary, small intestine, colon, peripheral leukocytes and liver (FIG. 5A). Additional RNA dot blot analyses indicate expression of BIVM in human testes, ovary, aorta, appendix, trachea, pituitary gland, bladder, uterus, spinal cord, salivary gland, stomach, mammary gland and bone marrow as well as in several fetal tissues (data not shown). Notably, BIVM expression was not detected in fetal spleen, adult thymus and certain cancer cell lines (e.g., promylocytic leukemia, HL-60, and Burkitt's lymphoma Raji) while significant expression was evident in other lines (e.g., HeLa, S3, and colorectal adenocarcinoma, SW480).

EXAMPLE 6--EXPRESSION OF BIVM IN OTHER SPECIES

[0155]The predominant mouse BIVM transcript also is ˜3.8 kb (FIG. 5B), of which ˜3.3 kb have been sequenced. Comparisons of 5' mouse BIVM RACE products indicate that the 5' untranslated region undergoes alternative RNA splicing, which, like in the human gene, does not affect the coding sequences. The highest levels of expression of mouse BIVM are in heart, brain, liver and kidney (FIG. 5B).

[0156]A major difference between the expression of human and mouse BIVM is observed in the spleen, in which expression is high in the human but appears to be minimal in the mouse. In the developing mouse embryo, BIVM expression is detected at a uniform level after gastrulation (FIG. 5B). An ˜2.1 kb XBIVM cDNA was identified in Xenopus that is consistent with the length of the predominant transcript observed in RNA blotting (FIG. 5C). The broad, diffuse nature of the principal hybridizing band could reflect sequence heterogeneity. The nature of the larger transcript (˜4.4 kb) is unknown. Northern blot analysis of sea urchin RNA detects two SpBIVM transcripts of ˜7.4 and 8 kb (FIG. 5D), which are notably longer than the human and mouse forms. The additional sequence in these transcripts might be a result of additional 5' or 3' untranslated regions and/or could reflect polyadenylation effects. Extended 3' untranslated regions are encountered frequently with sea urchin mRNA.

[0157]Real-time PCR was used to analyze BIVM expression levels throughout development in zebrafish (FIG. 5E). As observed in Xenopus and sea urchin, there is a large maternal store of BIVM transcript in the 1-cell embryo (0 hpf in zebrafish) which appears to be quickly lost after the initial cellular division(s). In zebrafish, the level of BIVM expression drops by ˜90% within the first 6 hours of life (midgastrula stage) and is comparatively undetectable by 12 hpf (post-gastrula stage). Although comparable stages of development were not examined in mouse (see above), it is likely that this early embryonic regulation of BIVM expression will be conserved.

[0158]We noted BIVM expression in chicken bursa, which serves as the primary site of B lymphocyte differentiation. BIVM expression in chicken bursa decreases slightly between embryonic day 12 and day 14, increases significantly at day 19, and is the highest in the 4 month old chicken bursa, in which levels are 6-fold greater than observed in embryonic fibroblasts (CEFs; FIG. 5F). Expression of BIVM in other tissues in chicken has not been characterized.

EXAMPLE 7--BIVM ENCODES A NUCLEAR/CYTOPLASMIC PROTEIN

[0159]The relatively high predicted pI of BIVM (9.1) suggests that it may bind other proteins and/or DNA (or other nucleic acids). The levels of BIVM produced from the native pBIVM-N2 construct and modified pBIVM-K1 construct (see Methods) were compared in whole cells lysates from transiently transfected Cos7 cells. BIVM levels are higher in cells transfected with the modified pBIVM-K1 (FIG. 6A), which was used in all subsequent transfection experiments. It should be noted that the size of this recombinant protein (with C-terminal epitope tags) is ˜61 KDa, whereas the native protein (without post-translational modifications) is predicted to be ˜57 kDa. The observation that a single protein is generated from this transcript argues that translation does not begin at a more 3' ATG as suggested by the "hypothetical protein" FLJ20159 GenBank entries (which are predicted to encode a ˜27 kDa protein). Western analysis using antibodies that recognize the V5 peptide sequence indicate that the epitope-tagged BIVM is present both in cytoplasmic and nuclear fractions (FIG. 6B). These results were confirmed by direct immunohistochemical localization of BIVM in the cytoplasm and nucleus (FIG. 6C-J). Variation in the relative amounts of BIVM in the nucleus was observed in individual cells. Thus, it is possible that the BIVM protein enters and exits the nucleus in a regulated or cell-cycle-dependent manner.

EXAMPLE 8--GIARDIA MAY HAVE ACQUIRED A BIVM ORTHOLOG BY HORIZONTAL GENE TRANSFER

[0160]A tBLASTn search identified a BIVM-like gene (named BIVML) in the genome of the primitive protozoan parasite, Giardia lamblia (McArthur, A. G., et al. [2000] "The Giardia Genome Project Database," FEMS Microbiol Lett 189:271-273). The 2045 nucleotide BIVML cDNA is predicted to encode a 270 amino acid protein (predicted MW ˜30 kDa; Pi=7.56) with no predicted signal peptide, membrane spanning regions or nuclear localization signal; thus, it is likely to be cytosolic. BIVML contains 17 cysteine residues (6.2%) throughout the protein (FIG. 7A). Known giardial proteins that are secreted to the trophozite surface or the cyst wall are also highly cysteine rich. This sequence is 22-25% identical and 46-49% similar to the carboxyl-terminal region of all deuterostome BIVM peptides described here, correlates directly with the conserved domain described above, and includes the M2 and M3b motifs (FIG. 7B). Northern analysis detects an ˜2.0 kb BIVML transcript as well as a larger transcript of unknown identity in both vegetatively growing and encysting cells (FIG. 7C).

[0161]BIVML is unusual in having long untranslated regions consistent with the size of the transcript. The 5' and 3' untranslated regions were determined by RACE and are 229 nucleotides and 983 nucleotides, respectively (FIG. 7A), most transcripts of giardial chromosomal genes characterized to date have very short (<20 nucleotides) untranslated regions, although exceptions are being noted.

[0162]The identification of a BIVM ortholog in such an early branching eukaryote was unexpected since tBLASTn searches of the currently available S. cerevisiae and Drosophila as well as S. pombe and C. elegans genome databases failed to identify any sequences exhibiting significant identity to BIVM. Furthermore, it has not been possible to identify BIVM-like sequences in the complete genomes of Campylobacter jejuni (Parkhill, J., et al. [2000] "Complete DNA Sequence of a Serogroup A Strain of Neisseria Meningitidis Z2491," Nature 404:502-506), Mycobacterium leprae (Cole, S. T., et al. [2001] "Massive Gene Decay in the Leprosy Bacillus," Nature 409:1007-11), Mycobacterium tuberculosis (Cole, S. T., et al. [1998] "Deciphering the Biology of Mycobacterium Tuberculosis from the Complete Genome Sequence," Nature 393:537-544), or Neisseria menigitidis (Parkhill, J., et al. [2000] "The Genome Sequence of the Food-Borne Pathogen Campylobacter Jejuni Reveals Hypervariable Sequences," Nature 403:665-668). In DNA hybridization studies, a Giardia BIVML probe failed to cross-hybridize to Trichomonas foetus, Trichomonas vaginalis or Entamoeba histolytica genomic DNA (data not shown).

[0163]The identification of a BIVM-like gene in the Giardia genome, but not in other similar proteostome genomes, taken together with the fact that Giardia is parasitic, suggests that BIVML may have been acquired via horizontal gene transfer from a higher eukaryotic host.

EXAMPLE 9--PHYSICAL LINKAGE OF HUMAN AND MOUSE BIVM TO THE EP58/MGC5302-EP58/KDEL1 GENE

[0164]The transcriptional start site of the human EP58/MGC5302 sequence (GenBank XM_--015844) is only 41 bp from that of BIVM: BIVM and EP58 genes are in a head-to-head orientation, in opposite transcriptional orientation. The mouse EP58/Kdel1 and BIVM genes share the same physical orientation separated by 224 bp. This exceedingly tight physical linkage and close spacing of BIVM and EP58 suggests that common regulatory elements located in or near the intergenic region potentially control the expression of both genes. RT-PCR analysis of extracts from BIVM expressing and non-expressing human cell lines indicated that EP58/MGC5302 was expressed in all cell lines that express BIVM but not in the BIVM non-expressing cell line, Raji (FIG. 8). Based on these results, it is possible that these genes are co-regulated and that the transacting factors associated with the 41 bp intergenic region linking these genes control their expression.

EXAMPLE 10--DNA BINDING ACTIVITY ON THE BIVM-EP58/MGC5302 41 BP INTERGENIC REGION

[0165]A MatInspector V2.2 search for potential binding sites contained in the 41 bp region separating the BIVM and EP58/MGC5302 genes revealed sites for cell type specific factors such as the myeloid zinc finger-1 (MZF-1), the hematopoietic-expressed Ikaros-2 (IK2) factor, and the ubiquitously expressed transcription factors NF1, USF, NFκB, and NMYC (FIG. 9). Nearly identical sites also were predicted for the mouse 224 bp Bivm-Kdel1 intergenic region. MZF-1 and IK2 are expressed in the K562 human erythroleukemia cell line and IK2 is expressed in the Raji Burkitt's lymphoma cell line. Based on this information, electrophoretic mobility shift assays (EMSAs) were performed to compare protein binding to the 41 bp region in nuclear extracts from BIVM expressing and non-expressing cells (FIG. 10).

[0166]MZF-1 and IK2-specific binding would be expected to produce unique bands in the K562 and Raji nuclear extracts that are not observed in nuclear extracts from non-lymphoid cell lines. In addition, an NFκB consensus sequence was used as probe and competitor (Santa Cruz Biotechnology) to detect bands representing NFκB-specific binding that may be constitutively present in the nuclear extracts (FIG. 10). Significant DNA binding activity was observed with the 41 bp BIVM-specific probe in all extracts assayed, producing 1 minor band and two major bands (FIG. 10; Lanes 4-10), one of which was competed by the addition of cold NFκB-specific probe, indicating that NFκB complexes may be present (FIG. 10; Lane 3). One major band was detected with the NFκB consensus probe in the nuclear extracts from BIVM expressing lines (FIG. 10; Lanes 13-17) that was competed by the BIVM-specific probe (FIG. 10; Lane 11). An additional complex also was observed bound to the NFκB-specific probe in the extracts from a BIVM non-expressing line (FIG. 10; Lane 18). Together these results show that the 41 bp BIVM-EP58/MGC5302 intergenic region supports DNA binding activity and that the bound complexes include factors that also bind the NFκB consensus probe. Similar DNA binding activity was observed in the BIVM non-expressing Raji cell line as in the BIVM expressing cells and may result from constitutive nuclear NFκB factors and suggests either that additional flanking regions function in BIVM gene regulation or that protein co-factors or other mechanisms, such as methylation-dependent promoter silencing, could play a role in BIVM expression. The presence of a CpG island 5' of the BIVM gene, together with the lack of both BIVM and EP581MGC5302 expression in the Raji cell lines, supports the latter hypothesis.

EXAMPLE 11--REGULATION OF BIVM EXPRESSION BY TNF-A OR OTHER INDUCING AGENTS

[0167]As described above, the 41 bp intergenic region contains putative sites for ubiquitous transacting factors and an NFκB site that appears to be bound by NFκB complexes containing c-Rel and RelB factors, which are constitutively present in the nuclear extracts from the BIVM expressing K562 cell line (FIG. 1). NFκB comprises a large family of transcription factors, most of which are sequestered in the cytoplasm through inhibitor binding. Activation of the cell by various agents, such as the proinflammatory cytokine TNF-a, leads to phosphorylation-induced degradation of the inhibitor and nuclear translocation of additional NFκB transacting factors. Although constitutive factors may drive basal BIVM expression, TNF-α activated NFκB increases the expression of BIVM in the BIVM-expressing HeLa cell line (DNS). Furthermore, a cell line devoid of basal BIVM expression, the Raji Burkitt's lymphoma line, is induced to express BIVM by TNF-a (FIG. 12). The specific TNF-a activated factors associated with the BIVM promoter can be defined using antibody shift assays.

EXAMPLE 12--CHARACTERISTICS OF RECOMBINANT BIVM PROTEIN

[0168]The BIVM encoded protein has a high proportion of lysine and arginine residues and a predicted isoelectric point (pI) of 9.1. The net positive charge under physiological conditions suggests that BIVM may interact with other proteins and/or DNA. Western blot analysis and cytoimmunofluorescence studies utilizing transfected, epitope-tagged BIVM expression constructs revealed that BIVM is present in both cytoplasmic and nuclear fractions. Variation in the relative amounts of nuclear recombinant BIVM was observed in individual cells and may reflect regulated or cell cycle-dependent BIVM nuclear import/export. The Cos7 cells that have been transformed stably with BIVM exhibit a decreased cell doubling time compared to untransformed Cos7 cells, suggesting the potential role for BIVM in cell cycle regulation. Furthermore, preliminary studies of Cos7 BIVM stable transformants stained with a nuclear stain (DAPI) reveal a high proportion of cells containing multiple nuclei compared to untransformed cells. Flow cytometer analyses of these cells stained with propidium iodide indicate that ˜90% of the cells contain tetraploid or greater DNA content, consistent with the presence of multiple nuclei (FIG. 13; Panel 3). This phenomenon was not observed in a G418-resistant, BIVM-revertant cell line, which has lost expression of recombinant BIVM and exhibits both a nuclear morphology and a diploid DNA content similar to that of the untransformed parental line (FIG. 13; Panels 1 & 2).

EXAMPLE 13--IDENTIFICATION OF BIVM PROTEIN BINDING PARTNERS

[0169]The high proportion of lysine and arginine residues and the net charge of the protein (pI 9.1) suggest that BIVM may interact with proteins and/or DNA (or other nucleosides). Specifically, protein-protein interactions are being assayed using the BacterioMatch two hybrid system (Stratagene). This system provides a rapid, selective approach to identify BIVM-specific protein interactions in vivo. Mouse Bivm has been utilized initially as we can take advantage of mouse cDNA libraries that are commercially available for this system (Stratagene) and because the results obtained can be used to complement concurrent BIVM knock out mice studies now underway in our laboratory. Although it is possible that BIVM may function differently in human and mouse, the 87% sequence conservation between human and mouse BIVM protein, strong synteny in BIVM flanking genes, and the tight physical linkage observed between the BIVM and EP58 genes, is consistent with functional equivalence.

EXAMPLE 14--MATERIALS AND METHODS

Example 14A--General Methods

[0170]RNA was isolated with RNAzol B (Teltest, Friendswood, Tex.) or Trizol (Gibco BRL, Rockville, Md.). Mouse genomic DNA (e FixII) and liver cDNA (e ZAPII) libraries were screened using standard procedures (Strong, S. J., et al. [1999] "A Novel Multigene Family Encodes Diversified Variable Regions," Proc Natl Acad Sci USA 96:15080-15085). DNA sequencing and the analysis of DNA sequences were carried out as described previously (Rast, J. P. et al. [1994] "T Cell Receptor Gene Homologs are Present in the Most Primitive Jawed Vertebrates," Proc. Natl Acad. Sci. USA 91:9248-9252). Alignments were constructed using ClustalW 1.8. Identity relationships were examined using BLAST and ALIGN software. Rapid amplification of cDNA ends (RACE) utilized a standard protocol (Mertineit, C., et al. [1998] "Sex-Specific Exons Control DNA Methyltransferase in Mammalian Germ Cells," Development 125:889-897) or the GeneRacer kit (Invitrogen, Carlsbad, Calif.). The RNA sources for RACE were: human HeLa cells, mouse liver, chicken bursa, Xenopus laevis liver, zebrafish (Danio rerio) liver, 15 hpf sea urchin (Strongylocentotus purpuratus) embryos, and vegetative-stage Giardia lamblia.

Example 14B--Genomic Mapping

[0171]Human BIVM was mapped using HSMAP5 (CCATGCCTCTCTACTACTCACTCCCAACAC) (SEQ ID NO: 51) and HSMAP6 (GGTAAGAAGAACACCATTGTGTTTGAAGGC) (SEQ ID NO: 52) intronic primers (which correspond to sequence between exon 8 and 9) and the GeneBridge 4 radiation hybrid (RH) panel (Gyapay, G., et al. [1996] "A Radiation Hybrid Map of the Human Genome," Hum Mol Genet 5:339-346) (Research Genetics, Huntsville, Ala.). Zebrafish BIVM (see below) was mapped using the zfBIVMMAPF1 (CAATGCCTAACACTGTGGAAAGTGAAGGCG) (SEQ ID NO: 53) and zfBIVMMAPR1 (GATAACTGTCGAGCTCGGTTGAGCAGGGC) (SEQ ID NO: 54) primers and the T51 RH panel (Glusman, G., et al. [1996] "Sequence Analysis in the Olfactory Receptor Gene Cluster on Human Chromosome 17: Recombinatorial Events Affecting Receptor Diversity," Genomics 37:147-160) (Research Genetics). Additional gene mapping data were derived from the Human-Mouse Homology Map and the Mouse Genome Informatics Database (Blake, J. A., et al. and Mouse Genome Database Group [2001] "The Mouse Genome Database (MGD): Integration Nexus for the Laboratory Mouse," Nucleic Acids Res 29:91-94).

Example 14C--Identification of BIVM Orthologs

[0172]Mouse BIVM Partial sequence of the mouse BIVM gene was obtained by screening a mouse genomic library with a human BIVM cDNA probe. A mouse BIVM cDNA was recovered by screening a liver cDNA library with a probe corresponding to mouse exon 6.

[0173]Chicken BIVM tBLASTn searches using the human BIVM sequence identified a chicken (Gallus gallus) bursal EST (GenBank AJ399198) encoding an avian ortholog (BIVM). RACE strategies identified a complete open reading frame cDNA. A single RNA-splicing variant, which encodes an additional 23 amino acids, also has been sequenced (GenBank AF411388; data not shown).

[0174]Xenopus XBIVM Partial Xenopus laevis XBIVM sequence was identified as an oocyte EST (GenBank BF047666) using tBLASTn searches with the human BIVM sequence. RACE strategies resolved a complete open reading frame cDNA.

[0175]Zebrafish BIVM Touchdown PCR (Don, R. H., et al. [1991] "`Touchdown` PCR to Circumvent Spurious Priming During Gene Amplification," Nucleic Acids Res 19:4008) and nested degenerate primers, designed with CODE-HOP software (Rose, T. M., et al. [1998] "Consensus-Degenerate Hybrid Oligonucleotide Primers for Amplification of Distantly Related Sequences," Nucleic Acids Res 26:1628-35), were used to amplify BIVM cDNA fragments from zebrafish liver. Primers for the primary PCR were designed to amplify the coding sequence between the amino acid motifs GNTTLMWRF and YFCPIGFEA; primers for the nested PCR were designed to amplify the sequence between motifs WFRQINDHF and YRHQNHYFCP. PCR products of the expected size were gel purified, cloned and sequenced. Full-length clones were derived by RACE.

[0176]Sea urchin SpBIVM A fragment of the sea urchin SpBIVM cDNA was recovered from 20 hpf embryo cDNA using nested PCR as described for zebrafish. RACE strategies identified a 1,899 nucleotide coding region that corresponds to the complete open reading frame of BIVM from other species; as of yet it has not been possible to identify a stop codon.

[0177]Giardia lamblia BIVM-like The Giardia lamblia BIVML sequence was initially identified with a tBLASTn search of the High Throughput Genomic (HTGS) database with the human BIVM sequence. BIVML is encoded in four overlapping genomic clones (clone KJ1556, GenBank #AC049185; clone MJ4898, GenBank AC083097; clone EJ2770, GenBank #AC038625; and clone KI0613, GenBank #AC046875). RACE was used to identify the complete, 2,045 nucleotide cDNA.

Example 14D--Transient Transfections

[0178]The coding region of human BIVM was cloned into pcDNA3.1/V5-His TOPO (Invitrogen) in order to generate pBIVM-N2, which encodes a BIVM-V5 fusion protein (the VS epitope is at the C terminus). A similar construct, pBIVM-K1, was generated in which the translational start sequence was modified in order to increase protein synthesis, as described in Kozak, M. [1996], "Interpreting cDNA Sequences: Some Insights from Studies on Translation," Mamm. Genome 7:563-574. Both of these constructs were then subcloned into pIRES2-EGFP (Clontech, Palo Alto, Calif.) to create pBIVM-N2/EGFP and pBIVM-K1/EGFP, which produced recombinant BIVM and EGFP from the same plasmid. Cos7 cells (˜60% confluent) were transiently transfected with expression constructs using the GENEJAMMER® transfection reagent according to manufacturer's instructions (Stratagene, La Jolla, Calif.).

Example 14E--Western Blots

[0179]Whole cell lysates were prepared from transfected cells in the presence of 1× Protease Inhibitor Cocktail Set III (Calbiochem, San Diego, Calif.) essentially as recommended by Santa Cruz Biotechnology. Nuclear and cytoplasmic extracts were prepared from transfected cells essentially as described in Yu, C. L., et al. [1995] "Enhanced DNA-Binding Activity of a Stat3-Related Protein in Cells Transformed by the Src Onco Protein," Science 269:81-83. Protein concentrations were determined using Protein Assay Reagent (Bio-Rad, Hercules, Calif.). Whole cell, nuclear, and cytoplasmic extracts were separated by SDS-polyacrylamide gel electrophoresis (10% polyacrylamide), transferred to Immobilon P filters (Millipore, Bedford, Mass.) and blocked prior to incubation with mouse anti-V5 monoclonal antibody (Invitrogen), anti-OCT1 polyclonal antibody (Santa Cruz) or anti-HSP90 monoclonal antibody (StressGen Biotechnologies Corp, Victoria, BC, Canada). Following incubation with alkaline phosphatase-conjugated secondary antibodies, reactive proteins were detected using Western Blue Stabilizer Substrate (Promega, Madison, Wis.).

Example 14F--Immunohistochemistry

[0180]Transfected Cos7 cells were fixed for 15 minutes with 3% paraformaldehyde, permeabilized in 1% Triton-X 100, incubated with primary antibodies, washed and incubated with secondary antibodies and 2 μg/ml Hoechst 33258. Primary antibodies included a mouse anti-V5 monoclonal antibody and an anti-actin polyclonal antibody (ICN Pharmaceuticals, Inc., Costa Mesa, Calif.) that were detected with a Cy2-conjugated, anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, Pa.) and a Cy3-conjugated, anti-rabbit antibody (Sigma, St. Louis, Mo.), respectively.

Example 14G--RNA Blots

[0181]Multiple Tissue Northern (MTN®) blots (human and mouse) were obtained from Clontech. In addition, 10 μg of Xenopus, sea urchin and Giardia lamblia total RNA were subjected to electrophoresis through 1.2% agarose, 2.2 M formaldehyde gels and transfer to nylon membranes (Zetaprobe®-GT; BioRad). RNA blots were hybridized with radiolabeled probes in Expresshyb® (Clontech). The Giardia RNA blot was hybridized with single strand-specific probes as described in Knodler, L. A., et al. [1999] "Developmental Gene Regulation in Giardia Lamblia: First Evidence for an Encystation-Specific Promoter and Differential 5' mRNA Processing," Mol Microbiol 2:327-340. Blots were stripped and reprobed with actin, 18S rRNA or Calmodulin probes.

Example 14H--Quantitative PCR

[0182]Real time PCR analysis detected BIVM expression from chicken bursa and zebrafish embryos and tissues using a GeneAmp 5700 Sequence Detection System (PE Biosystems, Foster City, Calif.) with SYBR Green detection. Each PCR series was done in triplicate. The relative expression levels were determined for each transcript from plasmid standards that were included in each experiment and normalized to the expression of S17 rRNA (chicken bursa) or S26 rRNA (zebrafish) levels.

[0183]All patents, patent applications, provisional applications, and publications referred to or cited herein are incorporated by reference in their entirety, including all figures and tables, to the extent they are not inconsistent with the explicit teachings of this specification.

[0184]It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application.

Sequence CWU 1

6513857DNAHomo sapiensmisc_feature(1)..(473)Exon A - untranslated 1agtaacgcct tctccaagtg gatggcgggg tggacacgcg tcccggcgcc ccgggctccc 60tgggatatgt agttcgcgac aggacgagcg gaaatactgc caggatttta ccacctctcg 120cccatttatt tacttctcgg tcaccgcttt cgggggacag ataaacacca cagatgccca 180tcaaaggggc gcacgggtct ggaggcgcag ctcaggtttt tgcgttggtc accctgccct 240ccgcacgtgg agagggcagg cataaagcac cttgaaagga aggtgctgtc aatgctatcc 300gacgacctgt cgccgggcac cgcagcatcc tcgctcgctc cgatgggacg agggacgccg 360gccccagggt aacaggaggc gcctcgccgg ccgcgcgctg gatgctgtga tccaggtccg 420gagccgggtt ccgccgcggc cgcagcgacc cgaccccacc cgacaggcca gaggaatcag 480tttagacttg aaattcagtt tttcctgaaa ctgatcagaa gttagtgaca ccttgattgg 540atccgttttt ctgtcaggag ctcattttgc agctctcaag cttttatagc atgctgtaaa 600caattgtcaa agttgtttat caagaaacag atagagttgc aacttgtttc tagtaataga 660aacttttaca ctgcattcaa tgcctaacgt tgcagaaaca gaaaggtcaa atgattctgg 720aaatggtgag cacaaatctg agagaaagtc acctgaagag aatctacaag gtgctgtaaa 780atctttctgc acaagtgcct caggagcacc cttgggtccc aaaggagatg gtcattatcc 840atggagttgt ccagtgactc atacacggga aaaaatttat gccatctgtt cggactatgc 900ctttctcaac caggcgacct caatctataa aactccaaat ccatcccgct ctccttgcct 960ccctgatagt acctctttat ctgctggaaa taattcatca agatacattg gtatcccgac 1020tagtacatcg gaaattatct acaatgaaga aaatagcttg gaaaacttat ccaacagcct 1080gggcaagcta cctctcgcat gggaaattga taaatctgaa tttgatgggg tgaccacaaa 1140ttcgaaacac aaatcaggca atgcaaagaa acaagtttcc aagagaaaaa cttcagataa 1200aaagggaaga tatcagaagg aatgtcctca gcattctcct cttgaagata ttaaacagcg 1260gaaagtatta gacctcagac gatggtactg cataagccga ccacagtata agacttcttg 1320tggcatctct tcattaattt cttgttggaa tttcttatac agcacaatgg gagctggaaa 1380ccttccacct attacccaag aagaagcttt acatattctg ggctttcaac ctccatttga 1440agatattagg tttggtcctt tcacggggaa tacaacactt atgaggtggt ttagacaaat 1500taatgaccac ttccatgtaa aaggatgctc ttatgttcta tataagcctc atgggaagaa 1560taaaacagca ggagaaactg cttcaggggc cctgtcaaag ttaacccgtg gattgaaaga 1620tgaatcgctg gcttatatct atcattgcca aaatcattat ttttgtccaa ttggcttcga 1680agcaacccct gttaaagcta ataaagcatt cagcagggga cctctctcac cacaggaagt 1740tgaatattgg atcttaattg gagaatcaag tagaaaacat cctgccattc actgtaaaaa 1800atgggcagat attgttactg atctaaacac tcaaaatcca gaatacctgg atatccggca 1860cttagagagg ggactgcagt atagaaaaac aaagaaggtt gggggaaatt tgcattgcat 1920catagcattc cagagactta actggcaaag atttggcctt tggaactttc catttggaac 1980cattagacaa gaatcacaac ctccaacaca tgcccaggga attgccaaat ctgagagtga 2040agacaatatt tccaagaagc agcatgggcg tctgggccgg tctttcagtg ctagtttcca 2100tcaggactcg gcatggaaaa agatgtctag tatccatgag agaaggaaca gtggttacca 2160gggttacagt gattacgatg ggaatgattg actatgcttg ctactgaaca gctggcatta 2220tatatgaaac tgctatatac aggactgtat aaagacagta gaagatttta gtaagcctac 2280attaaatagg agcagatctt gtggtataaa aaataacctt gtagttctcc agatactaag 2340cttgtatatg attatggtgg gtgatttcag atatataagc agataagcac agattattgt 2400cctttcaagt taagagtata taatctggac agaaaatttc acaaaattca ataaaattac 2460aactgttgtc taaataagtg aaacacaaat tcacttaata gcatcaagat ttgaaatact 2520taagcatgaa gtgactttta taatgactcg atccctagac atttgttaca gatagtttta 2580tgcctaagac caagatgtaa agtaccatct gcccttaaaa aaaattgggg ctgtcaattt 2640ctagttttca ctcatggtta acacgcattt aaaattattt catgagtcta gtagttcttt 2700gatttatagc aggatcttgc ttgcctcatt tgtttcctgg ttatgttctt aggattctga 2760ctaagaggca aaagagaaaa gactcaagaa actgatcctg gagatcgaga ccatcctggc 2820taacatggtg aaaccccgtc tctactaaac atacaaaaaa ttagccgggt gtagtggtgg 2880gcacctgtag tcctagctac tcgagaggct gaggcaggag aatggcgtga acccgggagg 2940tggagcttgc agtgagcgga gatcgcgcca ctgcactcca gcctgggcga cagggcaaga 3000ctctgtctca aaaaaaaaaa aaaaaaaaaa gacggatcct tttttttggt gcaaatgggt 3060gacttagtgc attgattcag atttttaaaa tttcttgatg tggtttgtaa taatcaaata 3120ttgacaagaa ccttaggtct cgaaagactt ttataagtct agatgacgtt tgccttaggg 3180gtaaagtaaa agaacaattg gcaccttaag tttctatacc caaggttatc tgtgaaatga 3240gatctcctga tatttgattg ctttctcagt atggagtcat atgttgataa cagtactgaa 3300gatgcataag aaatgcccaa gtcactcaga ggacaactac ccatattcca gactctgagc 3360tgtttccttt ttaaaaatca tatagacaat tagctgtttg aagtgagtat taaatatttc 3420agaagtgtga atttcatgta tttgagctcc tctagttgct gttggttttt cttctgctgc 3480caacctgtga ctcacaaatg actaggatct cttgttcttt aattttaggg tcttgttcca 3540ggactcaaat cagtaacttg gtgattacaa ggtgctgaat gtgttggtaa ccatatcgca 3600atacacctca aggaaaaggt tcagattttt atttttaaaa tattttcatt tttttcttga 3660attttatatc cgtttgttca ctcgtacatg cctagcctac agaaggggat atatattatg 3720aaatggtcat ttttctgaag agaatatttt gcttgaaatg caaaggactg aaagagattt 3780gtaggttgtt gattttgtta cttcatactg gaacttttaa aaagatttca tcaaataaag 3840ttttgttttc tactttt 38572503PRTHomo sapiens 2Met Pro Asn Val Ala Glu Thr Glu Arg Ser Asn Asp Ser Gly Asn Gly1 5 10 15Glu His Lys Ser Glu Arg Lys Ser Pro Glu Glu Asn Leu Gln Gly Ala 20 25 30Val Lys Ser Phe Cys Thr Ser Ala Ser Gly Ala Pro Leu Gly Pro Lys 35 40 45Gly Asp Gly His Tyr Pro Trp Ser Cys Pro Val Thr His Thr Arg Glu 50 55 60Lys Ile Tyr Ala Ile Cys Ser Asp Tyr Ala Phe Leu Asn Gln Ala Thr65 70 75 80Ser Ile Tyr Lys Thr Pro Asn Pro Ser Arg Ser Pro Cys Leu Pro Asp 85 90 95Ser Thr Ser Leu Ser Ala Gly Asn Asn Ser Ser Arg Tyr Ile Gly Ile 100 105 110Pro Thr Ser Thr Ser Glu Ile Ile Tyr Asn Glu Glu Asn Ser Leu Glu 115 120 125Asn Leu Ser Asn Ser Leu Gly Lys Leu Pro Leu Ala Trp Glu Ile Asp 130 135 140Lys Ser Glu Phe Asp Gly Val Thr Thr Asn Ser Lys His Lys Ser Gly145 150 155 160Asn Ala Lys Lys Gln Val Ser Lys Arg Lys Thr Ser Asp Lys Lys Gly 165 170 175Arg Tyr Gln Lys Glu Cys Pro Gln His Ser Pro Leu Glu Asp Ile Lys 180 185 190Gln Arg Lys Val Leu Asp Leu Arg Arg Trp Tyr Cys Ile Ser Arg Pro 195 200 205Gln Tyr Lys Thr Ser Cys Gly Ile Ser Ser Leu Ile Ser Cys Trp Asn 210 215 220Phe Leu Tyr Ser Thr Met Gly Ala Gly Asn Leu Pro Pro Ile Thr Gln225 230 235 240Glu Glu Ala Leu His Ile Leu Gly Phe Gln Pro Pro Phe Glu Asp Ile 245 250 255Arg Phe Gly Pro Phe Thr Gly Asn Thr Thr Leu Met Arg Trp Phe Arg 260 265 270Gln Ile Asn Asp His Phe His Val Lys Gly Cys Ser Tyr Val Leu Tyr 275 280 285Lys Pro His Gly Lys Asn Lys Thr Ala Gly Glu Thr Ala Ser Gly Ala 290 295 300Leu Ser Lys Leu Thr Arg Gly Leu Lys Asp Glu Ser Leu Ala Tyr Ile305 310 315 320Tyr His Cys Gln Asn His Tyr Phe Cys Pro Ile Gly Phe Glu Ala Thr 325 330 335Pro Val Lys Ala Asn Lys Ala Phe Ser Arg Gly Pro Leu Ser Pro Gln 340 345 350Glu Val Glu Tyr Trp Ile Leu Ile Gly Glu Ser Ser Arg Lys His Pro 355 360 365Ala Ile His Cys Lys Lys Trp Ala Asp Ile Val Thr Asp Leu Asn Thr 370 375 380Gln Asn Pro Glu Tyr Leu Asp Ile Arg His Leu Glu Arg Gly Leu Gln385 390 395 400Tyr Arg Lys Thr Lys Lys Val Gly Gly Asn Leu His Cys Ile Ile Ala 405 410 415Phe Gln Arg Leu Asn Trp Gln Arg Phe Gly Leu Trp Asn Phe Pro Phe 420 425 430Gly Thr Ile Arg Gln Glu Ser Gln Pro Pro Thr His Ala Gln Gly Ile 435 440 445Ala Lys Ser Glu Ser Glu Asp Asn Ile Ser Lys Lys Gln His Gly Arg 450 455 460Leu Gly Arg Ser Phe Ser Ala Ser Phe His Gln Asp Ser Ala Trp Lys465 470 475 480Lys Met Ser Ser Ile His Glu Arg Arg Asn Ser Gly Tyr Gln Gly Tyr 485 490 495Ser Asp Tyr Asp Gly Asn Asp 500396898DNAHomo sapiensmisc_feature(466)..(702)Inverse complement of MGC5302 Exon 6 3ctagacagag aaagcaaacc ctaattccca gacagaacat ttggatgagt ttcacgtcat 60tccccaccag aaggcctggt aagaaacaag tagtttatat cttccttatt ctgttgtgat 120aacatggctc tgggaaaaag tatttgtaaa atgacttaca tgtaagtatt aataaaaaca 180tatttggagg tgacatgtgc ttatctcaag ggttagctgg aaaaaaatta tagctctatc 240caggctccaa actcccttga tgcgcattta atatcaagac tgggcagtga gggcagaccc 300tggttgccaa aacagtcccc agcaccccca tgtctcaata ttcgctcaat tttgtgctaa 360cttctcccac ctcttgaaat ttgcaggcct taatttccct tcccaaagca ctatgtacaa 420atacattaga aaaacaaaaa agattagcta ctgattaagt cataccttga agaaatcaaa 480aaatgaaata tgtttcacaa tgggaccata caggttttca tcgtgtttaa agaagaaaaa 540gttggtgaaa gcagcgtcta tgagttctgg gtgttttcta ctgagtttaa ccagctcgag 600tctctctttg cggctgtctc gccctctcca gacggcagtg gaatttttgc tttcccaggg 660aggacccgtg ttagcttgca cggacatcat atccagactt accctagaag acaaagtgca 720acagattttc ctcccaaatc atcatatcac aaaggttgtt gcaaaagaac tcaccaaaga 780aagaaaacca aggctctggg ccaaccctga cttatctctc accggcccat ggtttccaga 840acagaatcag tcaaatcgta cgtaggcatc acgatatcct tggaatctgt ggagccacac 900caggaaaaga tcggatggat gtttgaattg gatttctttt tttccaaagg ccagtctccc 960aaattaacaa agagctccac atctggcatc ttcacctaga aaaaacaaaa cgaggagctt 1020attcacattt cctgcattgg ataatacatt gtatttatca atttaaattt gaaattgcat 1080taatgaaagg ccaatgttac atgtttcagg taataataat taccttggtt tgaatagtgg 1140gcaatgtttc agaatatggt cacatgtata agtattctat gctccctatg aaagggatga 1200aactgcaaaa gtaatctctg ttctaatact gcgaacagcc tctaagatga tcaaagagaa 1260ggagaattat tggaaaataa tttaaaaaat agcacttggg actctaaccc tagtttcctg 1320ctagtgagcc agatggttcc attttaaaaa acatacacca ggtaggtggt gggaccagga 1380tttgaactca ggaagcccaa caccaacagc aacgtagtaa gtttcaagct atgcttcctt 1440cctctactgg caaatggcat gaatatgtaa agaggatatg ttttatctag tcacagaaaa 1500tgtttagagt atttacaaaa caacagatat acatttttaa ggccagaaaa gcactgacca 1560gtctgagaag catcttgaga aaccgaagtc ctgaagaaca ttctcatttt cccatggatt 1620gacaaagagg agcaagagaa gtatgacggt actctgatgt cttagtttaa aggaggctta 1680atattgatgc tatataacta cctacattca gaattaacag actgtaagtg ctttgaaatc 1740ttgaaaaaaa ggcattatga ttttccatga gtagtttaat caagatatac atgcaaatta 1800ttcaacaaat aaatataata gattacaaat aaatatacat ttggtaaaaa tgttggtaga 1860gtctttcaca tccaccattt taacattact ttcaaaccat tccactagaa cccaacaaaa 1920gcccattatc ccaaggaatg ggattcagta caaggcaagt catctttgga ctcagagtta 1980gtttagtacc aaaataattt tatgataagg catttttctt tcctctataa aatattgtgt 2040ccctgcaaaa gatatgctga agtcttaaat cccagtgtct cagaatgacc ttatttggaa 2100atagggttgt tgcagatatt agttagcatg aggtcatact ggagtaggct gggccattaa 2160tccactatgc tgtgtcctta taagaagaga cccagagaca tgaggggaga agcccacgtg 2220atgacagagg cagagatggg aggatgcagc tgcaaaccaa ggaacaccaa agatggaggg 2280ccactgacag cagccaggaa gaggcaggaa aggaaaggaa aggatcatct cagagggagc 2340tggcctcctg acactttaat tttttttggg gggggacagg atctcactct tgtcacccag 2400gccttgggag tgcagtggcg tgatctcggc tcatgcagcc tcgacctcct aggttcaagc 2460gatcttcctg cctcagctcc ccattagctg aaactactac aggctaattt ttgtattttt 2520tgtagagaca ggtcttcacc atgttgccca gcctggtctc aagctcctgg gctcaagtga 2580tccacccgct ttgacctcct aaagtgctag gattacaggc gtgagccatg gcacctagct 2640gacactttga ttttggacat ctggcctcca taactgccag agaatacatt tctgttattt 2700taaggcttcc agtttgtagc actttgttac ggcagcccta ggaaacaaac gcaggtacct 2760gaaataacag aaagcttcag aatagtatta tcataaggct ccagaagaga ataccctgat 2820agtgtataat ttgtattttg aaaaattatc ctgtaaaatc tgggcttaaa ttatctatta 2880cagctaagag aaaatatata cttacctttc tagtcaaaga aagtagtatg gcatccatga 2940aaattctaaa acctacatgt tcaccatgag tcttgatata aacctaaaag agaatttacc 3000atttattatg ttagtctaaa gacgctggca gacttcactg aggaaaagct tgtcacagtg 3060ctcattcgaa tgatgtttat aaaatgattt agctaattgt agccaaatgt tcaaaacaag 3120aaaaaaaatc actaaaacaa acaagcaaaa aatcatgtgt tgtaccttgt tatcctttaa 3180ggtgtagtga cataggctct gcctctgtcc aaatcttttt gggatttcta ctgcaatctt 3240ttctggatcc acagcaggga aatgtgccag atctctctga atctgagcaa tggtttcagg 3300gcagttcatc tcccgtagcc aggctgcact atcttgcaga ggacagtcac agttctcatg 3360gtaaaccggc cctatttaca taagaataac aaagtacaac agtgatcatt tcactcagca 3420ttcgaaactt gtaataaaca tctataatgt ggcttcatga attttgctta gttacatagg 3480aagagcattt tcattgaaaa cacattttaa gagaattaca ggccagcaag gttggctcat 3540gtcggtaatc ccagcacttt gggaggctga ggcaggaaga cagcctgagc ccaggagttc 3600gggagcagcc tgggcaacac agggagacca tgtctctaca aaaaataaaa aattagctgg 3660gtgcgttggc atgcacctgt ggtttcagct acttgggagg ctgacgtggg aggatcactt 3720gagcccagga ggttgaagct gcagtgagcc atgtttgcgc cactccatcc tgggcaagac 3780agtgagagcc cgtttcaaag aaaaaaaaag taatacattt ttactatact ggggcattgc 3840aagtaagtca gccttctact ctgacagctc ttcacagtga agttttgtga tattttcata 3900ataataaggt ctactttgca tgagttcaaa agaaaggaaa tagaggctgg gtgcagtggc 3960tcacgtctgt aataccagca ctttgggagg ctgaggtggg tggataactt gaggtcagga 4020gtttgagacc agcctggcca acatgttgaa accccatctc tactaaaaat acaaaaaagt 4080ttagctgggt atggtggtgt gtgcctgaaa tctcagctac tcaggaggct gaggcatgac 4140aatagcttga atctgggagg tgaaggttgc agtgagcgga gattgtgcca ctgtgctcca 4200gcttggggga cagagtgaga ctcttgtttc aaaaaataaa agaaagacaa cagatacttg 4260gtttatgtct atggtaatat tacagctcat tattccttat gttttgcaga ctgaagaata 4320accaaatact ggtaaaaggg tatcagagga gcatacctat tttaatgaaa gacaaaagtg 4380atatgattta ctattttgat ggtctgttat acaaacaggt tcttaaagtg tctaaggtac 4440ttttctcaat aagtaaatca tcaataacag caaaaataaa caaggaactg ctattctttt 4500tttttttttt tttaagaatt attaaggagg aaaagtaggc tgggttcttg gctcatgcct 4560gtaatcccag cactttggga ggctgaggcg ggcggatcac gaggtcagga gatcgagacc 4620atcctggtca acatggtgaa accccgtctc tactaaaaat acaaaaatta gccaggtgtt 4680gtggcgcgtc cctgtagtct cagctatttg ggtggctgag gcagcagaat cgcttgaacc 4740cgggaggcag agcttgcagt gagccgagat tgcaccactg cactctaggc tagtgacaga 4800gcgagacatc gtctcaaaaa aaaaaaaaaa aaaaaaagca aaagtagctg ggtacagtgg 4860ctcacgcctg taatcctagc actttgggag actgaggtgg gcagatcact tgagctccag 4920ggttcgggac cagtctgagc aacatggcaa aaccctgttt ctacaaaaaa tacaaaaatt 4980agtggggcat ggtggtgagt gcctgtagtc ccagctactc atgcagctga ggtgggagga 5040ttgcttgaac ccaggaggtc gaggctgcag taagcagtga ttgcaccact gcactccagc 5100ctgggcaaca gagcgagacc ctgtctcaac aacaacggca acaaaaagaa gcaaaagtaa 5160ttctcaaaac agtccacttc actaatttta taacaaatta attacagtct gcactgaggt 5220ttttactgtt attccttttt ataattctca gatcccacct aacccaggca gtggctgaca 5280atggaatatc tttttaaggt ttagtgggtg atactgtacc aggctgtact ggcagaatgt 5340aggaaaggaa cctagacact cttgaaaagt gtttaccttt tcttacttct ctgcagagtt 5400cacaaaaata aaaaaaaaaa agtttacttt tcttggggtt gttaaggggg ggacaagatt 5460tctgcctttg tatatacact gcttccctac tgtcttgtgg tactgtcgcc tgtaagaggg 5520aaggagatgg ctctaggtaa taaaactgta ctctcatcct atataagaaa catcagaatg 5580gccgagcatg gtggctcaca cctgtaatcc cagcactttg ggagactgag gcaggcagat 5640catgaggtca agagatcgaa accatcctgg tcaacatggt gaaatcctgt ctctactaaa 5700aatacaaaaa ttagcctggc atggtggtgc gtgcctgtaa tcccagctac atgggaggct 5760aaggcaggat aatcgcttga acccggaagg cggagcttgc agtgagccaa gactgcgcca 5820ttgcactcca gccttgtgac agagcgagac tctgcctcaa aaaaaaaaaa aaaaaaataa 5880ataaataaat aaataaataa atgtcagaga atttctctgg gaaaatggca ttggaacaaa 5940gacaaaaaaa caaccaccag tgcgctcctt gtctttcgag ctatctccct tcctgaagtg 6000atctacctaa tttcaataac acttatcagt tacttaagtc atatatttcc aattaagaaa 6060gtatcatata tgagcatgaa cacagcttga ttattcttgc taatgtatgt cttcgcggag 6120taaattctct actaatatgt tccttgctct ttagcaaatc agaatttccg ttcaaatctt 6180gaatgtcttt ttaccaggac tcataactta gctttcaagt agaaagcctt tatttttctt 6240ctttcagtaa aaaaaaaaaa aaaaatatat atatatatat atatatcatg ccaagttgtt 6300tttgttgatg gaataacata tattatttta ttcaaatact gctgttataa aattatattc 6360caaattacct tttaaaatat atggggattt ggccacatgt tgcccttgga atttaatttc 6420caccttcaga tttttgtagc ttgcatacat tctgtatctt actatgaagg acccatcttt 6480tcggtctaaa acctggactc caactctagt gaattgctcc tctggtgctg agactttcac 6540ctggaagacc ttttcgcctg gagaagatgt gaatctgtga tgaaaaggca atgggggaga 6600taaacagatt ttaacgaaca aacacacaaa ggttatgctc ttcagtctgg aaacttgatt 6660attctcctcc acattcttcc tctaacatat gattgcttct tcttctctta tgcttttagt 6720accagaatga atatgatgga tcagagattt aaaaaaatga ggatgacaat ggccaagata 6780acatgaaaca gtttcattaa acagtcacaa tggagtatgt taagactatt aatctatata 6840agcacagaac atactcatgc attccatatg gaaaaatatt cttactaact tatttcatgc 6900tatcacatct ggatatttgt gatcaaatga tgctcccaaa gataatgatc ttattttgaa 6960aaatctctgc caatgaatct ttacaatatc agtattataa atcacgctca ggcctggcac 7020tgtggcccat gcctactatc tcaacatctt gggaggccaa ggagggaaga ctgcttgagg 7080ccaggagttc gagatcagcc tgggcaacaa ggtgagatcc tgtctctaca aataataata 7140ataataataa aaaacaaata gccaggcgag gtggcacacg cctgtagtcc caactactag 7200ggaggctgag gtgggaggat agcttcagcc tgggaggttg agggtacagt gagctgtgat 7260tgcaccactg cactccagac taggctagga agtgaaaccc tgtcttaaaa aaaaaaaact 7320cacgctcagc catgggcaaa agcaaagtag taggtagtgt atcatctttt gccaaataac 7380aaaatttaca acctgccttt tacttgacaa ccttttattc cagtattttt ttctgtatga 7440aatacacaca gtcagcatcc accaataata gagacaatca tttcagaagt ataggaatta 7500taaaaataag ttttaagtat gatgttaaag tattaaattc attggaacaa aatatttgtt 7560acaatcatat aggagattaa tacttcgata ctttccttcc gaactctgaa gtttcaaata 7620gtacttcatt tttctttttc agtttcaaaa catgcttacc tgcagatgtc tccttgaaaa 7680ttgagggccg caccagagaa agatgaagcc catcaagtct ctaacaacta ggtttaaatc 7740ccttataata gcttatgcga aaagtagtta gaaacagtat ttccagcaaa gtggagggac 7800tttgtcttgc cctgataatg tgggaatctg aatcaaaatt gctaggagat tattttatat 7860gttatataca atacattata ttgcatatat tataattcgg aacaatttct acagctacag

7920agaaaaatgg gtaaccaaat atcctggaat ataaactgct gtggagaaac gagttcttgg 7980aaagaaaaat cttaaaggga gaaacagatt taaccgccat ccaaaatagg taaggagccg 8040tttctcgact tacttattcc ctgatgtatc cactgcctga atatagaaat agcgggcggg 8100aaggacgacg tctgctttta gcccgggtcc ccatatttcg ctcttctccg ggctcagctg 8160cctttctccg ccggtctcgg cgagtgctgg aactgtcgcc agaaagaagc aataaagtag 8220caaagtgcca aacatttaca agacggactc tcgaaatgat ccaccgataa atgaagaagt 8280gtaagaggtg gacagaagca gccgagcctt cggcagggac ccgccggccg atcgcagcag 8340ccaacgcgac tgcaaaggtt gccgcccggc gtgcagggca ggcgcgcggg tctccgcgac 8400cccaggacaa tcaaagcccg tgccccggcg cgcccaggtg agggtcccct ggcgttctgc 8460tgtcccggcc gagaaccgcg ctgctcctct ctctcaggac aatgatgaac ttgagttgct 8520cctgccactg gagcatcatt tgggagcgaa tccgtctcag gttccagcca aggtgtaggg 8580cgaggggatt ggcccgtgcg tcgggccagg ctcagtaacg ccttctccaa gtggatggcg 8640gggtggacac gcgtcccggc gccccgggct ccctgggata tgtagttcgc gacaggacga 8700gcggaaatac tgccaggatt ttaccacctc tcgcccattt atttacttct cggtcaccgc 8760tttcggggga cagataaaca ccacagatgc ccatcaaagg ggcgcacggg tctggaggcg 8820cagctcaggt ttttgcgttg gtcaccctgc cctccgcacg tggagagggc aggcataaag 8880caccttgaaa ggaaggtgct gtcaatgcta tccgacgacc tgtcgccggg caccgcagca 8940tcctcgctcg ctccgatggg acgagggacg ccggccccag ggtaacagga ggcgcctcgc 9000cggccgcgcg ctggatgctg tgatccaggt ccggagccgg gttccgccgc ggccgcagcg 9060acccgacccc acccgacagg ccagaggtac cccggggcgg ggggcagggg ccgaggtggc 9120ggccggctgt gcgctctgag cgcctggccc tccgctgggc acctgggcgc cgccagcccg 9180gcctgctgcc gctctacgcg cagccacctg ggcattcaaa atttttactt aattcgatac 9240cggcctgggc tgccaggggt catcgcctct ccgagccccg tggcgtccag atggaggcca 9300ctgcatgggt gcgcgctctc ccgggaagga gtagggggaa gagctgtgtc gcggggggaa 9360gagaagcgcc agggaaagaa gggcctagcc ctctggtgaa caaagctcga ttaggaggtg 9420tccatgtgga taccggtgac ccctgtgcgg ccgtcggtct ccacgccacg ctgggcaggg 9480tccgggaacc agcgcgcagc ggccgtcgcc ttcccctgca cgccagcacc cgggtctggg 9540ccgccgccag gagtcacggg ctcaccgccc tggtcagctt ggcagtcgga cccggagccg 9600cctcctctgc ctgcctccct cttgccagct gccccgaaaa cccagaagag ccggtggctc 9660cgagccaagg cgggcctggt tcggcgccag gaaagggggt ttctttccta ttttcttttg 9720tgcaattgtc attattaatg ataccgactc gtttactcaa acagtcgaat cggagcccca 9780gctcttagcc cggatgcagc aattgcccgt gggccccctt taacaccaac agcgtccccg 9840gggcccgggg caagcatgtt cgaggcggtc acccccgggc ctcggcgcgc tccccctggc 9900ggagagcctc gtcttccggc cggtgaggga aggtagagga gggagtaggg gcgaggaggc 9960ctcggcggcc cttgggctct gcgggctggg gactcggggt gcccgcgaca cgcgcggagg 10020cgcgggctgg gttggccacg ggcagggagc gcagccgcgc tccttcctct ctgcccgcgt 10080cgcctccgcg cgcactggtt ctgcgcggcg gggcttggcc tgcgcgactg tctactccgt 10140cccggcggcc tcggagcccg gccgagcggc gagcttgtca gaggacggtg gtggaaacgc 10200tcccggcctc cccaggggcg cgggctggag gctggcgcca ggcgcggagg actcccggta 10260tcttttgaca ggctggcgcc tcggctctgg ggacccgcag gtctgaaggg gaggaagggg 10320cctggagggc gcgggaggac accgggtggg aagggtggca ttagctcggc cgggggctat 10380gcgcctctgg tttcgccctc ccgcgcatat tcgaccctta cgaggtcacc ggaatgcccc 10440tgctcctcag ttgccttcta tacaggatat cgatcagggt attttgttat acgaaaaggc 10500tttactgaag aggtttttag agatgtttgg ttctctcata aacttgatac ttgagaatac 10560agacaaaata taacctgaaa agactacaac ctaggcgatg aagattggct ttacaaatgg 10620acgtttattt tacagaacac ttcgttcagt gactttgaac aatcatgact ctggcggtgc 10680tttttaaact tgccatttta taaatttttg ctttgcatac gagcaaacca tatttctatt 10740gcttatgaca tgattttatg agtaagctat tagttgagcc tgaggtcctg cagtcattct 10800tagtagtaaa tttttttttt tttttttttg agacggagtt ttgctctgat cgcccaggct 10860ggagtgcaat ggtgcaactg taacctccac ctctggtgtt cagcgattct ccagccttgc 10920ctcctgagta gctgggccta cacgcatgcg ccaccatgca cggctaattt tgtatttttt 10980tattttttat ttttttttag tagaaacggg tctcaccatg ttggccaggc tggcctcaaa 11040ctcctgacct cagatgatcc acccgcctca gcctcccaaa gtgctgggat tacaggtgtg 11100agccaccacg ccggcttagt aaatcttaat atagcaacac ctcacttgcc tggaagaggg 11160aaccgcaatc aatcaaaatg agggcctaca gtaatgcctg gcatgatgca aacacttaaa 11220aattatctgt tgaatgagac gtctacaaat cctaggccct ggggatacaa taatctggaa 11280aaccagactt gcaggatgca gacgttgatc atatgaacag atatgcacag aagtaagtgt 11340aaaattgcca cctggtaaga cctgtgtgag gaaggtacta gagtctgtac cgggtcacct 11400ggcctagttt gagaagccaa gaaggtttcc cccagaaagt gacatttgag ctgacattgg 11460aaagatgaat gggaatgagc taagtaaggg agacagtatt gggagaacca aaaagtaatg 11520tggagcttgg ggggtggggg aaaggaatga gatggagcta accagataga tctagggacc 11580atcaggagtt ggccttttgt tctaagagca gaggctttca ggcagaggag ttctgtgatc 11640atatatatgt agcaaacttt attttctaat atctctgcac aggtcaggtt agaagtgtca 11700actcactggg aatagtttat aaatagagaa acaaaaggag ataaaagatg agtcaaaaca 11760gtgacagctg cagcctatta agtggaggga caaactgcct ctctatagct cagtattgtc 11820tataatgatt ctgttattag tattatcagt aataaattgt gcttagtgta ctttaagaaa 11880gctagaatct gagcatgcaa taatagaagc ccccttggcc tcttggggct ctcactatag 11940tggagagaat agacgtgaga cagtgtggaa agaaagtaaa cactagcagt gtttgggtcg 12000tgggatttgg gtaatttcca ttttcctgta atatcttttg gtactttgca tttttttgta 12060atgttttact tataaaatct atgaatatta cattttcaaa gagaaattta catatagttt 12120ccaatgagaa tgtttcatgc ccttggattt tagtgacagt caatataaaa tgcatcctta 12180tattgatgat cttcattttt tttttgctaa aacttcgacc aaataaatca tcttgttccg 12240tgaccattat ttaaaagcaa acaaactaaa aacacaaaca aaccagactg ttactttttt 12300ctctctttcc tttttttttt ttttttttga gacagagtct tgctttgttg cccaggctag 12360agtgcagtgg tgtgatcata gctcactgca gcctcaaact cctggcctca agtgattctt 12420ttgcctcagc ctcccaaagc atagatatta caggtatgag ccactgtgcc tggctcacac 12480tgttactctt tttattaatc tagtgctgtg ttctatcttt agcgtccagg aagcttaccc 12540ccaacttttg tgcttaaatg cagtcatttc cctttgccta tgtttttgat aagaatattc 12600tccatggctg ggcatggtgg cttgtgcctg taatgccagc aatttgggag gctgaggcgg 12660gaggattgct tgaagccaag aggtcaagac cagcctaggc aacatagcaa gaccctgttt 12720cttaaaaaaa aaaaaaaaaa aggttattct atatatgttc caaatgagca tacttttaca 12780atccctgcca ggtgcagtgg ctcattccta taatcccagc actttggggg gccgaggcca 12840gcagatcacc tgaggtcagg agttccagac cagcctggcc aacgtggtga aaccccatct 12900ctactaaaaa tacaaaaatt agccaggcat ggtggcacct gcctgtaatt ccagctactc 12960cagaggctga ggcaggagaa tctcttgaat ccaggagaca gaggttgcag tgagctgaga 13020tccggccatt gcactccagc ctgggcaaca gagtgagatt ccatttaaaa aaatcaaatc 13080aaatccctac actgtcacac agagagctgg tcccacaggc aaaattccat tcagtgtgag 13140gaaggaagcc ctgggaaagt ggaagccaag tctgagatga ggatataaaa ggggcagggc 13200ctggaacatt tccgtctcgc caccaaactc actctaataa cctttgtcta ttgcctctca 13260ccgagactat atgctctttc attcctcacc tcgcacagcc cacccccacg accccaatac 13320cacaaatacc tacctctctg tccaccacac tgatgtagag aaaggcatga aggtcacaga 13380tgagaagtag aaaatgctat gttaggacat ctgctgagaa tcagagcaac tctgtcttcc 13440aaaaagacaa gagtttggtc tgaacaacgc caggtactga gcttccctct gccatcaccg 13500ttgcaccacc agatgaataa ggagagagca ccacttccac ttgaggaccc actacaacta 13560ctccaagaat tttttttacc aaaagaaagt gaaagttttc aaagtgaaac cacaggaggt 13620tccacctttc gtggtaatat tcctatccaa ctgaccctct tgcaaacaac tataaactct 13680gcacaaatta ttttaaaact gaagagtttt ttgtttgttt gttcgtttcg agacaagatg 13740gagtgcaggt cacccaggct ggagtgcagt ggcgcgatct tggctcaacg caacctccac 13800cttcgagcct caagcaattc tgcctcagcc tcccgagtag ctgggattac aggcatgcgt 13860cactaccgcc tggctaattt ttatcttttt tttgagatgg agtttcactc tgtcacccag 13920gctggagtgc aatggcagga tctcagctca ctgcaacctc cacctcctgg gttcaggccg 13980ttctcctgcc tcagcctcgc aagtagctgt gattacaggt gcgcgccatc atgcccagct 14040aatttttttg tgttttttag tagagacagg gtttcaccct gtgtgccagg ctggtctcga 14100actctgacct catgatccac ccgcctcggc ctcctacagt gctgggatta caggtgtgag 14160ccactgcacc cggcccttac tgtctggcta atttttaaat tttcagtcga gatggggttt 14220caccatattg gccaggctag tcttgaactc ctgacctcaa atgatccacc cgcctcagcc 14280tcccaaagtg ctgggattac aggcatgagc caccacacca ggcctaaaaa ctgaaggttt 14340gaatagagaa aaagcatgct ttaaaagtaa agaaaatgga attttgccta gcatatgtgg 14400agtcctaata tgcagctctg tttccttaaa ttccatgaaa gccatgcagt acctttgcta 14460gtttctcctc acagatcagg ataacctagg gggctcttgt gtgaatcgtc ttctatttct 14520tgcagcctaa ctcataggct ttcgttgttc aatatttgta tgatggtttt gatactattt 14580ttggtaaccc atgacagtta tttttatttc taatttttta agtaagcaaa tgggcagaga 14640tattaactgg taaaagtcca actgatcacc cagggtggac tgaatctctc aactgatgct 14700ctgttgctgg agccctgaga aacccgcata ccctgcccgg gcacctgcct ggggttgtct 14760gctgcgtgtc ctgggatggt tcaattcacc aaggacttcc tctggtataa atcttcagct 14820tccttgcatg ccctcagttg ctatttaagc tttctgtttt cttccctaaa ggaatcagtt 14880tagacttgaa attcagtttt tcctgaaact gatcagaagt tagtgacacc ttgattggat 14940ccgtttttct gtcaggtgat gaatctttgg aaaatttact ttctgtattc tgtgtttatt 15000taaatctgtg gccgttattc atcatgtatc ctttatgcct atgtacgtaa aaaatcttgc 15060taatacatta ttttttagac aactttatgg aggtataatt cacacaccat ataatttacc 15120cattaagtta tgcaattcat tggcttttag ggtatttaca agttgtgtgt tcattgccac 15180aatcatttat agaatattat cattattaca aaaagaaact ccatcacccc caaaccccaa 15240gccctaggaa accatgaatc tactttctgt ctgcatagat ttgcctgttc tggacatgtt 15300atataaatag aataatacac tatctggtcc tttgtgacga tcttctttta ctcactataa 15360tgttttcggg gttcatccat gttgtagcat gggtcagtac ttcatttctt tttattgcca 15420aataatattc cattgtatgg atataccaca ttttatttat acattcctca gttggtggac 15480atttgggttg tttccatttt ttggccatta tgaataatgc tgctattaac atttgtgtga 15540agatgtattt tcatctgtca cggatatata ccttggcatg aaattgctgg accatatggt 15600aactctgttt aattgttgga agaactgttt tccaaagcaa ctggaccatt ttacatttcc 15660attagcaatg tatgagggtt atgatttctc cacgtcctca ccaacatttt tgattatagc 15720cattctagcg tgtgtgaggt gttaatctca ttgtggtttt gatttgtatt tccatgatgg 15780ctaatgatac tgagcatctt ttcatgtgct tattggccat ttatttttat ttttgataca 15840gtctcgctct gttgcccagg ctagagtgta gtggcgcgat ctcggctcac tgcaacctct 15900acctcccagg ttcaagtgag tcttatgtct cagcctcctg agtagctggg actacaggca 15960tgtgccacca tgcctggcta atttttgtat ttttagaagg gacggggttt caccatgttg 16020gccaggctgg tctcgaactc ctgaccccaa gtgatccacc tgccttggcc tcccaaagtg 16080ctggattaca ggtgtgagtg actgcgcctg gccttattgg caatttctgt actgattttg 16140gagaagacac tattcagata ctttgcccat ttttaaaaat tgggctattt gctggccggg 16200catggtggct cacatctgta attccagcac tttgggaggc agaggtgggc agatcacttg 16260aggtcgggat tttgagacca gcctgaccaa gatggagaaa aaacatctnt actaaaaata 16320caaaattagc cgggcgtggt ggcgcatgcc tgtaatccca gctacttggg aggctgaggc 16380aggagaatca cttgaacccg ggaggcggag gttgcggtga gccgagattg tgccattgca 16440ctccagcctg ggcaacaaga gtgaaactcc atctcaaaaa aaaaaaaatt gggctatttg 16500ctttttaatt attttttaat tatttgaaaa taatttaatg catattttag actaatttaa 16560aaaataagat agtgattgtg actccagtca tatagtagtt gtaaaattaa tatagaatga 16620aggcatatgt atgcataaaa cttgctatgc tttttagtgg ctctttgtgt atctggtgga 16680ttgttgatca ttctttttcc ttcctcttag gagctcattt tgcagctctc aagcttttat 16740agcatgctgt aaacaattgt caaagttgtt tatcaagaaa cagatagagt tgcaacttgt 16800ttctagtaat agaaactttt acactgcatt caatgcctaa cgttgcagaa acagaaaggt 16860caaatgattc tggaaatggt gagcacaaat ctgagagaaa gtcacctgaa gagaatctac 16920aaggtgctgt aaaatctttc tgcacaagtg cctcaggagc acccttgggt cccaaaggag 16980atggtcatta tccatggagt tgtccagtga ctcatacacg ggaaaaaatt tatgccatct 17040gttcggacta tgcctttctc aaccaggcga cctcaatcta taaaactcca aatccatccc 17100gctctccttg cctccctgat agtacctctt tatctgctgg aaataattca tcaagataca 17160ttggtatccc gactagtaca tcggaaatta tctacaatga agaaaatagc ttggaaaact 17220tatccaacag cctgggcaag ctacctctcg catgggaaat tgataaatct gaatttgatg 17280gggtgaccac aaattcgaaa cacaaatcag gtaaggaggg agccatgaag ttcatatgtg 17340aaaataatga gaaaacaaac actatgtctt gtttaatctt gccattacac atagtttcct 17400tgtataatac tagataagga acatggctat catcttgtct gtcaatgtag ttttaggaaa 17460gtaaccttga cgtagggcat gtagttcatt gcggggcttc cactggaaac ttcaagcata 17520agctttgtat caaatatttt gagagatttg aaaatctaat aatgtaaaat attataaaca 17580gatggtagct tagaaaatga aatgttaata acatggctag aataacttac tactgtttca 17640tagttttata ggcacatgaa gttgtatttc ctgaccaaac atcttttttt cctgctatat 17700aatgttttag ctttttttgt tgttaaaatt ttttaggcac ccagcaaagc ctccatgtac 17760caccaagtgg gttgtgtact gctcaattta agaggatgct gtttaccgag gttgtgcata 17820actttcacag ttgcaatggg gtggtcctat ggcagataga aatactttac acttctttct 17880tttgaattca aagtaataca ggaattttat gaggcaggta ctgttagccc catttgtagg 17940tgaagaaatt gaggcttaga agggataaat ctctagaccg aagttgcaga gttaataaga 18000ggaggtcaaa ctaggatttg tatttacttc tagtcttctc tgatgatttt ataaaacctt 18060aatgcttctg cttgtttatc tgcaaaatca atttgtttca tagaatttta ggtaaatttg 18120taattcttaa gggtaggagg gggattttgc tttttttgtc taaatttgta ggtaatgagt 18180ctgcaatttt ctttttgtct aatgcctttg gccagttttg gaattagggc tatgcaaatc 18240ttctaagaaa agttgggaag tgttttttcc tcctgttttc tgaggatttt cagagagata 18300ttaaaatctg tatttataat tatggagttg tcttgatttc tgttaatttc tgacaaattt 18360taatttgtcc atgtcattta agttgtcaaa tttgggggct taaagttaat gttatccctc 18420taacatctaa ggaatctttg ttgacaactc cttttgcatc ctaatactgg taattaattt 18480attttctcta ttttttttaa aactgatcaa tctagctagg agtttatcaa ttttgttaat 18540cttttcaaat aactagcttt tgttaatttt ctcaattgtt tgttttctat ttcattggtt 18600tctgctcttt attatttgct tcctctgact tactttgggt ttattttgtt cttcattttc 18660tgacttctta agatggaagc ttagatcatt gattttagac ttttctacca taagcataga 18720atactccaaa ttgctaagta ctgctttagc tgcagctcac aaattttgat atgcttatta 18780ttattactta attggaaata ttttctaatt tcctttgcaa tttattcttt gatacatgta 18840ttacttagat gtatgctgtt taatttctag atattaatag tttttctaaa tattgatttc 18900tagtttagtt ccattgtgga cagagggcat atgctctcag tctttttaaa tttactgaga 18960cttgttttat gacccaacat atggtctatc ttggtgaatg tgccatgtgc acttgaaaag 19020aatgtgcatt ctgcagtcat tgggagtatc tataaatatt aattatgtcg aagtgtttga 19080aagtgtcatt cacatctttt gtgtctccgc ttaacttgtg tcttgttcta tcaattacca 19140aaagaagggt gttaaaaatc ttcaactatg attgtgaagt tgtcttttct ccatttaatt 19200tcttttttaa aaactaatac atgtctaata acagaaaatt tactgtctta accattttta 19260cgtgtacagt ttagtggcat taagtacatt tacgttgttg tgcaaccatc atcactattc 19320atctccaaat ctctttcctt ttcttttctt tctttttttt ttttttctga gacagagtct 19380cgctctgtcg cccaggctgg agtgcagtgg cgcgatctcg gctcactgca agctccgcct 19440cccgggttca caccattctc ctgcctcagc ctcccgagta cctgggacta caggcgcctg 19500tcaccgtgcc cggctacttt tttgtatttt tagtagggtc ggggtttcac cgtggtctcg 19560acctcctgac ctcgtgatcc gcccgcctcg gcctcccaaa gtgctgggat tacaggcgtg 19620agccaccccg cccagccctt cttttcatct tacaaaataa actttgtacc cattaaacaa 19680taaatcctca ttccttctcc tcacagtccc tggaaaccac aattttactt tttgtcactg 19740tgaatttgac tattctaggt accttatgta agtgaaatca tatagtattt gtctttttat 19800gactggttta tttatagcca cccctgctct cttctgggtg ctatttgctt tggaatactt 19860atttccatcc ttttactttc agcctatttg tatctctaga tctaaagtga gtgtttcaga 19920gacagcatat agttagctaa ttttttctgt ttctgttttt gtgtgtgaac ccacgtatac 19980aaaaacatat ttttaaaaaa atgcattctg ccagtctcta tgttttgagt ggagaattta 20040atccatttac atttgaagta atcactgata aggagagact tgtcatttta gtacttgtta 20100tttatatgta aacactctgt tcctatactg cttcgtccac acttaacttt cagttattga 20160tgttatcaaa ttacaaattt atatattgtg tgtctgaaaa cataaactaa taatttttat 20220gtattaatct cttaaataat gtggaaaaca aaatgtggag ttacaaacca aagttattat 20280aataatagct tttttatttt tattttttat tttgaaatag ggtcttgctc tcttgcccag 20340gctggagtgc agtggcacga ccatggctta ctgcagcctt gacctcaggc ttaagcaatc 20400ctcccacttc agccttctga gtagctggga ctacaggtgc acatcaccac gactggctaa 20460tttttaatat ttttttgtag agatagagtc tcactatgtt gccaggggtg gtcttgaact 20520cctgggctca agcaatcctc ctgcctcggc cttccaaagt gctgggaata caggcatgag 20580ccactgctcc tggcctacta ctagctttta agctaataat aatttctcaa aaatgtatta 20640gtctcataaa tcatatagaa tacaaaaact ggagttgcaa actaacaaaa taacactggc 20700ttttataatt gttcgtgtat taccttcact gaggtaaatt tatttcttcc tatggctttg 20760aggtactagc taatgtcctt tcatttcaac cagcaggaat ccttttagca ttccttacag 20820ggcaagtcta gtggtaataa actccttcag tttttgtttg tctgggaatg tctgagtttc 20880ttcttcactt ttgaaggaca gttttgctgg atttagaatt cttgtttgaa tttttttttt 20940atttcagcac tttgaatata tcagcccact tccttctggc ttccaaagtt tctgatgaga 21000aatctgttga tattcttatt aacagtccat tgtatgtgat gagttgcttc tcttgtgttg 21060cttttaagag tctttgtctt tttgcaattt ttaaattttt tatttgatac ggagttttgt 21120tcttgttgcc caggttggag tgcaatggca cgatcttggc tcaccacaac ctccaccttc 21180tgggttcaag caattctcct gcctcagcct cccaagtagc tgggattaca ggcgtgcgcc 21240accatgcctg gctaattttg tatttttagt agagacggag tttctccatg ttggtcaggc 21300tggtcttgaa ctcctgacct caggtcatcc gcctgcctca gcctcccaaa gtgctgggat 21360tacaggcgtg agccaccgtg cccagccttg caattttatt ataacatata ttggtgtggg 21420tctttctaag ttcatcctat ttggaatttg ttgagtttct tggatgttta tattcatgtt 21480ttttaaatca aatttggaaa gtttttaggc attatttgtt tggataatct cctacccctt 21540tttcagtgtc ttctccttct ggaacttctg caatgtatag gttggtccac tcgatggtgt 21600ccccaggtcc cttaaggtct gtttactttt cttcagacct ttttttttct gttcctcata 21660ctcaattatt tcagttttcc catcttcaag gtcactaatt tttctgcctg ctcaaaactg 21720cctttgaatc actctagtga gtttttcatt tcagttatta tatttttcag cccaagaatt 21780tatttttggt tttaagtttt ctatctcttc attgatattt ccatgttctt catacataca 21840tctttcgtta gttcttttgg catctttaag atagtggttt taaagtcctt gtctagcaag 21900tctgccattt ggtctttcca ggatggtttc tgttggttta tatatttttt tgtttctttg 21960aatgggcctt attctgtttc ttgtgatttt ttgttgttgt tgttgttgga aactagacat 22020tcaaatatta tttaataatg cagtaactct ggaaatcaat acccatcttt cccagagttt 22080gttgatttta gtttttgttt tttgattgct ataggctgtt tctatgctgg taatcaacct 22140gaggtataca tttaagggct tctcagggaa aagcccctgc ctttctctga gtgtgtacag 22200tgattttcta catttccctg tataagtgat tgcttttgaa tatactgttt ttgaaatgtc 22260tggctcccca aacagaaaat ggagaggaaa aaaaacagaa caaaaaaacc acaccaaagg 22320tgctggcccc ttaagtcctc tggaagttgc ttctgttgta gtgggagggg cttgcaacat 22380tggagggaga gttgcaacaa tggctgcccc cctgtatctg tgctttcaag atcagaagca 22440gcagtcagca atcagaacac agatttccaa ttttcatagg acatggacct ttttgcccac 22500catggttccc acaaactgcc tgaagcttct ccaggagcat atgcacagtt tcttggactt 22560ggggataggt agttgtacct tgctatgtgc tgaaattgac caaaatttgc atttactgtt 22620caggacttcc tctgaaagtt gcaatccttt gaatagactc tggaattcca aaataattac 22680atcagacaga ttctgccagt gcagttgtct aggtggggag acagattcct ggtgcctcct 22740acactgcact taattctgtt aattttttta aaaatgtaat ttgaagctct gttattaagt 22800tcacatacat ttatgagatt taggccttct tcctgaattg acccttttat cattatgaaa 22860tatatttcgt tattcctgga agtatttctt gttctgaaag ttactttgtt tgatattaat 22920aaagctcctc agctttattt tgattatatt tgcatggtat atatttttcc atctatttat

22980tttagttcta gaatttacat ttttatagct tctatttctc atctgagatt tgctatattt 23040ttcatttatt acaagagcat tttatctatg tcataatgca gagttataat atctacttta 23100atttcttatc tgcaaatttc aaaatctggc taatctcaca gtaggcctta tcttttttct 23160tgagaatgag tcatattttt cactttcctt atatgttcta taattttgca ttgtatccta 23220gagagtggga ctattatgtt atggcaactc tggtatattc cttcaaagtg ttgatttttt 23280tttggttttg ggaggcagtt aatttggttg aactaaaact gtaaattctg tctccttggt 23340acagctccaa ccatctcagt tcagctcttt tagctgtgtt gcttgaattt gctccttaca 23400tgcatggtcc aggggtcagt ccaaggtttg ggccaaattt atacacagaa tttgggcccc 23460ctcttctctg tggctctctt ctttctgggt ttcctctcac ctcactttca gtgatggtga 23520ttgcacagga ctctgtcctt tggttcttcc agtaaaaaaa gacagtggat tttctgcttt 23580tgtttgctct tctcatacct ttttctaaaa gtcagtgtgg tgaatacata tatttgtgtg 23640tgtatgtgtc tgtgtgtgtc tgtctgtctg tgtgtgtgtt tggtgttgag gctgtgtgtg 23700tgtgctgtgg gtagggtgag gaatggaaga aggaactcag taatgagaag gagcagtggg 23760cgaaggttct tgaagctgat gtgatggaag gcagaaggtg caggtgttgc tcttctatgt 23820tcccatctca aacggcgctt tcactttgat gtggcttctt tccctcaagt caaatgaact 23880tttgcctgtg ggaccagctc tttctgtggc agtgcgggca ttttatgaag tacgctcatt 23940tgggccgtat atgctaattg atcccagggg caatccgaga ttcaacaaaa agagaatagg 24000gggcaaggtc atggaaccct agaggaaagt gtgagtacag actgttggca gagcctatca 24060aggtcacaca tttctatgtt tcattagctt taacatttta agtggctggg aaaactactt 24120agggaattaa gtcattatta ggcagtgaag acatctcaag aaatgttaga aactaattct 24180tctgttatct gactctgtaa gagtcatttt accagtcaag gaaatttggc acaatgagat 24240ttggcacagt tgctcacctt tgccagtgat gttcagctct gtgaaaagtg tgttctctgt 24300aaaaacttag aaaaaataat taaaagggtc acgcatgatg gctcacactt gtaatcccag 24360cattttgtga ggctgaggca ggtggattgc ttgagctcag gagtttgaga ccaacctggg 24420caacatagtg agaccctgac tttgttaaaa atacaaaaat tagctgggca tggtcgcacg 24480cacttgtggt cccaggtact cctgaggctg atgtaggagg attgcttgag ccccggaggc 24540agaggttgca atgagtggag attgcgtcac tgcactcttg tctgggcaac aaagcaagac 24600cttgtctcaa aacaaacaaa caaacaaaca aacaaaaaga ataattaaaa ggaaggcgaa 24660acattgtttc ttgactttat agtcttcatt attattacat ttttacagaa attccctgtg 24720taataatagt tcctgagttc cagctgttcg taggtgtcaa atggtttctc tgtatagtat 24780cttgaaggaa taaaactgat ctctttccat gtttgcttac taggcatata tgtatataat 24840ctattttata atttatgaat gactcataaa aatgaaatat tagccttcag ttaattttta 24900taacagaact gttttaaaat agaatatgtg tgaaatatta aagtatttga gcatagctat 24960ctgaaatctt aatagtattt taatgaaatg aggcttggat gttatttatt gatattttac 25020tttatataat tttttactag atacttcact aacgatttag aaataaaact tataaaaata 25080aaaagtatag ggatggatac cccattctcc atgatgtggt tatttcacat tgcatgcttg 25140tatcaaaaca tcttatgtac ccctaagcat gtacacctac tatgtactct caaaaattaa 25200aaaaaattaa aaagtacaaa ttctcactct tacttttggc ttccttttgt ctagctccca 25260attctccctt ccccagtagt aaccactgtt gtttgccttg agggtctttt ggagaatgtt 25320tgtatgtgtg tgcatgtatg agtgtgtgtg cacatgcata catagatgca tacatgcaca 25380caggaggctt gcttttcatt ctagtttctg tgctagagtt gcattttagt aatgtatatg 25440tgtgcatcat tcttcattca ttctgtcgtc tctgtcggtc cagagatatt tttaaacatc 25500acatcatcac attaatggat ctggtgcttt tactttctta acagagttgc tttaatttgg 25560ttttattgtt ttatagaaag ccttcatttt ctggaaagtg gtctagttaa atttttttac 25620tgtcgataat ttgattttcc ggatgtggct aaagatttag tgattgactg ttccaccagc 25680taagtgacta gctctggaat tggagtataa gaacctgaca cgaaatagga ttcatggttg 25740tttaaatttg tttttattat ttctctttaa ggtaggggac tattattgaa aatacatgta 25800ttacttcatt gtgtttgcaa atatcacttt tagaacctgt ggcagttatt tatttgttat 25860tattttttct ttgcactttc tcagcagaat tattaaggca gttacttctt aattttggtg 25920cataattaat ttttgtcatt tcagtttact tcatcttaag cattttgctt attttatact 25980cttgtattct aggcaatgca aagaaacaag tttccaagag aaaaacttca gataaaaagg 26040gaagatatca gaaggaatgt cctcagcatt ctcctcttga agatattaaa cagcggaaag 26100tattagacct cagacgatgg tgatgttatc agtttttatt tttttcattt ttgaaatatg 26160taatatatgt tggcaataac gtagcagttt acaaattaat acattatatg tatcataaat 26220atttttattt ctaaaattat taccaggatt tctgaatatt taaagtcacc acaaatgttt 26280gaagtagatt aataatgagt tatgctggtc tttcccaaac tcggcttatt attagagtca 26340tctgtgtgac tccacgtaac tgccatgtag atttaacaat gattaacctt ttgatgtagt 26400taaatactta gcttttcatt gctgaagaat tttcaagtaa gtgactgatt tttattatat 26460ttcatctcaa gttacctaag tatgcatctc taaaaaatga cttttttcta cttaaccaca 26520atacttttgt catacttaat aagattaacg atatttccct agtgtctaat tcccaggcta 26580aattcacgtt tcccagattg ttctgaaaag gtcttcacat ctgtttgttc aaagtgggac 26640ccagttgtga accacatgtt tgcttgtcat gacccataat tctctcaatc taggtcagtc 26700tccaccccac tttccccctt gtcttttaaa aattctatta ttttaatcga caaatgagaa 26760taataaagat tgacttattg aagagacttg gccagatatt ttatagaatg tcctacattc 26820cagatttgta ttttcctttt agtttcctaa attatgcatc taacctcaat atttcctgta 26880aactggaagt tgaatacaaa gacttgatta aattccagta caacatttta tgcaagaaca 26940ctgcttaagt gggccctcat acttcaaagg tttcacatca gaaggcacat ggtatctgat 27000tatccagtat tagcaattct aagtgattac tgggttaagg tggtgacagc tgtattctac 27060attgacgagt tctttctttc tcctctggta ccctcaagta atcagtagag tgacagagtg 27120gcattaggta atagccaggt tccatcagct ttttacctta tggttttagc caaatgtatt 27180gacaacccaa acccattgat gatccttgtc tgaatatttg ttgcaaaatg gttatatttc 27240aaaattccaa tttttttttt tttttacttt tattacccag ccttcttctg tagctttcac 27300tgatcaactg gggctatttg gttatcttaa aatacagttc ctagtctaat gaaaacaagt 27360ttaattcatg acaactttta tgtattactg accctgattt gggtttttaa aattcctttg 27420tatgacattt tagaaatata tttttatcac ttatttaata aatattggac cctttgctgt 27480aggctgagaa acagagatgc acacaacatt gtctttgttt tcccaaggtt tacaatccag 27540ttgaggtgag gggactaaac agaaaaatgt taaatatctt aggcagtttg tcatgtgatg 27600ggtatgaatc actaattaac catgaaagga ggtaatgatc accagaatgc tttactaagg 27660tcctgggtct taagggagga aatatgaagg gttggagggt gaagggtctt tgaaagaatt 27720acagcaaagt gtctctggaa tgtgggtaat gaagagtaga gtagcttgtc taaagtgagg 27780ctcagagagg ccaagaaacc tgccagtgtc acatggctaa taagtagaag atcttgtatt 27840gaagctgaag agtcagactc aacattcatg ctgtttgcgt ataatacatg tcatgatctg 27900tttcttagat atcaggctca atatttagat tttttttctg agttaaagat aagccatgga 27960cgatttctta acaaaaggaa cttttatcaa gataaatttg gaatagttag tatgtaggga 28020ataatggaga agagacatta aagtcacaaa cattcatttt agtttctttg aaaagaaggt 28080tttgctcgtt tgtgcttcta atcacttaga tttaagcttt gtttcaacaa caaatctgtg 28140taactacata ttaatagtat aacatgtgag gccatgaagg ctaggactag gaaggataga 28200tgggagaaat gccctgaggg gaaaacatca ccaggactat aagcctgttt tccactgaga 28260atattacaag attaggttag aatggaaagt cccgtagaag atagtagaat gttggtaagc 28320attttaacct ggtcataaac aagactagga aacaagttta gattgatgaa aaagtatgaa 28380aaatcttaag agaaattccc ctttccctct cctttttaaa aattctttca ggccaaattt 28440agtgttatga gaaaaactat atacatgcat gcaatacttt gttagtaaat atatgtaaag 28500ataaatgttg taaaatataa aacactgtta gtattggact tgtaaaataa tccccaaaat 28560tgaactctgg caaaaatatt cacagcagaa aaaaaaaatc agtaaatgaa tgagttgctt 28620gtgttttatc agcaagggcc atatagctta tgatgacaga actgttaata atcacttgtc 28680cacaaaggtc tcttaggttg gagttcacac tcatatttaa acaactaggg cagattactt 28740gaataaggac attagtaaat tgctcatgtt tccagggata ggggaaaggg gtaaaaggga 28800gttgtgttgt tcagtgggta aagagtttca tttttgcaag atgaaaaagt tctgttgcct 28860aacaatttgc atatagttga cactactgac ctgtacactt aaaaatagtg tacatgataa 28920atttgatgtt gtatgttttt atcacaatta aagaacaaag caaaactgag ggaggactca 28980atagatttga cagtaaggtg ttgaagacag cacttagtat gttaagtgat agttattagc 29040ttttgtgtgt cattttgtgt agtctacaga tttagggctc gtggcacacg ttctcactga 29100taagtgagag ccgaacaatg tgaacacatg gacgtaggga gggaacaaca cacactgggc 29160cctgtcgcgg ggtgggaacc ggggacagga gagtatcagg aaaaatagct aatgcacaca 29220gagtgtaata cctaggtaat gggttgatag atgcagcaaa ccaccatgac acacatttat 29280ctatgtaata aagctgcaca tcctgcatgt ataccctgga acttaaaaaa aaatattgaa 29340gggtcatggt taacttgtat gtgtgccaat atgcatagta tatatacata tacaaatgca 29400tatatgtaca catgcatatg tctaattttt cattttgtaa ttaattttta gaccacacta 29460aagttgctct tagtgatgat agggcttgtt tctttgtttc attgtggcta cttcttagca 29520ccttctttag aaagcagtta ggagaagatc atttgaaggc caacgagtgt tgtgggttat 29580tgttaagctg atttaacatt atctcccccc acaaccacgc ttgactagct tcacatttgg 29640ccaggtgcag tggctcacgt ctgtaatcca gcactttggg aggctgaggt ggaagatcac 29700aaggtcaaga gattgagacc atcctggcca acatagtgaa accctgtctc tactaaaaat 29760acaaaaatta gctggttgtg gtggcgcacg cctgtcgtcc cagctactcg ggaggctgag 29820gcaggagaat tgcttgaaca cgggaggtgg aggttgcagt gagccaagat tgcaccacag 29880cactccagcc tgggcgacag agtgagactc tgtctcaaaa aaaaaaaaaa aaaaaaaaaa 29940gagaaagcaa acgtatttct tcttaaaaca gaataaataa atagtctgtc tctttctcct 30000tctgttcaca tttgccccag tttcttctct tgaatcatga cagtttggaa aattgtctgg 30060attgcttagt gccactgaat catgccatgg aaggattttg tatttcactt ttaaacttct 30120ctgtgacagg agaagcactg cttcatggct tcttgcccaa ggattttaga tggacacagt 30180gggtaataaa tggatgaatt tttgtttggg ttgaagaatc tctctgagaa gttgacacgt 30240gggggcaatg gtttgtttct cttgtatttc tgaagttgca aataatcatg taagcagttc 30300aaccaggagt ttacaccaaa cttttaatag gcgatatatc attatttttt ttcccattgg 30360tttggataac atccacttta actggcagtt agtcatactt agctattttt gttaaagcag 30420gtgatttatt gttattttat atttatgaca tgattaataa gtgaatatgg aagattttac 30480attgacttag gggatcaaag ttttcattat attaacacct ttaattgcca tgagttttct 30540atttctagca tgcatatttt gtgttcattc aagtgaagaa aacagtcttt tgtgttctca 30600ggtactgcat aagccgacca cagtataaga cttcttgtgg catctcttca ttaatttctt 30660gttggaattt cttatacagc acaatgggag ctggaaagta agtatgtcaa tttatcagta 30720cccccaaact ccaaagtaat ttgatgttgc tttttctata acagaaaaaa atttaagaat 30780agatttttta taaatttaac aaaaccctgc tgtattttag tgtaagtctt ttagcttaaa 30840atatatacat gtattatttt cagtgaaata aaaatgggct gggtacagtg gcccacactt 30900gtaatgtcag cactttggaa ggccaaggcg ggagtattgc ttgaagccaa ggagtttgag 30960accagcctgg gcaacaaagc aagaccccac ctctacaaag taaaaaaata aaaacaacta 31020gccaggcaca atggcatgta cttgtatttc tagtctctta ggagactgag gcaagaggat 31080cacttgagcc caggagttta aggctgcagt gagctatgat cacgccactg cactccagcc 31140tgggccacag agtgagaacc tgtctctaaa aaaataaata aaataaaata atgaaaaata 31200ttactaatat tatttgcaaa tcagacaagc atattaacat tgagacaggc tgtatttgcg 31260tatgactgga attgaaaaat gaaaggcaat gaatgtttct tttgtagcct tccacctatt 31320acccaagaag aagctttaca tattctgggc tttcaacctc catttgaaga tattaggttt 31380ggtcctttca cggggaatac aacacttatg aggtatgaag accctcttag aggcaatatc 31440gtgtttctag ttttgcaaaa taataatgat gtagatgtgt gttgatagta aaccatgtat 31500ccagctgctg ggcttcaacc tctcataggt ataccaactt tgggctatgc ctgaatttct 31560ttagaattgg aataatgcca tctttgttgt agaattgttg ccatgaacaa atcttcttgt 31620tactttcagg tggtttagac aaattaatga ccacttccat gtaaaaggat gctcttatgt 31680tctatataag cctcatggga agaataaaac agcaggagaa actggtaggt aaacatatag 31740aagatttaca tacacacata cacacatgca cacacacaca tacacacaca cggtttagag 31800ttcgtctcaa agactgcctg tttagcttcc tgggacatta tgtaaaccct cagcacagtc 31860tgttacttgc atgtaatctg tagggcactt tttaataagc agtttaatat tttacctctt 31920ctaacctttt tatgtagaac ctttagaact ttaaggtata tcacaaacac tactctaaca 31980cattgtattg tttgggtccg tggtgtgtgt gtgtgtttgt gtgtgtatgt atgtgcgtgt 32040gtacgcgcac atgctagtaa aactctaagg aagcagcatg gagttagcag ttttcttcct 32100aagaaggaat gtaaggattt gagaatattt gatgttagac ctgtgtggtg acatataatg 32160gcaggaaaat agactgttga agtgacaaag ttctgctata aaatagtctt gtttataaca 32220gaaatattaa tggagttgat ctcaaggatt attttagtta tgtgctaatt aaataatttg 32280tactatttag caatttccat ttttaaaatt gttacaatct tttgactgtg taaatcagaa 32340aattgtaata cattgttaaa gaaaaaatta ttctgacact tgttaatagg gtgagaagac 32400tttattttaa acttgtgaaa ggcctattgt agtaggaatg agagatcagg ctcagctctg 32460aatacagcaa agacaacaaa gacaactggg actttatagc caagggacac agcgaggggt 32520ttgggggtgg gatattacta agagggtcaa gggtatgggg attcttgcta aagcctaaca 32580gacttcttgc taaaggcagg tcaagggctg agacatcagg agttggcagg ggacgtccgt 32640aaggaacttg attagatatt aagggttgtg ggttctctct aaactgactt agcaggattc 32700atgctaaaac tggacacaga agtctaaggt ccaggcctgt ttgagaagag gactcaagga 32760ttaatcagga agagaattgt tatcaataga gggtatatgg tttaccaagg taaaagactg 32820agcacaaagt cagatatatt tctgttgacc tatattgggt atatcaggtt ggttacttgg 32880aggcaggctt ggagattaca tgttgctttc tacacatact agttttgtga ccttgaacaa 32940gtcacttgct gagccttgat gtagaccttt tatctttcta tatttgtata gggtcagtgt 33000gaggataaaa ggagacatac ttgtaatgta cttagcacgc tgtgttacag agtaaggact 33060cagtaagtgc tagcatttat tcctactgac taaattctat gcattcacat ggggcctgtc 33120tatccttctg gaaaacacag ctttaaatgc tgtgttgcta tttatttctg tttctagtga 33180gactgtcacc acactactct cagtaagtga tcaggcaacg aaaaactaag aaaggatata 33240ttatcctgtt tggtaatttt ctcttcagct gtgtctagtt tgctttttat actgcctatt 33300gagtttgagt tttttaaaag aggttttcaa tcatttttat tctaaaatat gtagttcttt 33360taaaaatctg cttttaaaaa taacctcatt ccttactcat attttcaaac ccttttattt 33420tttaaatcta ttgaaacttt ttcctctgta tttggtgtaa tgtaggaaat gtgtatatat 33480ctgattttgc tgtctcttgt ttcttctgga tcttatttga ggtgtcttcg tgtgccatga 33540aaagaaaatg cataatttgg cattgtcttt tatagttctt gaatgtttac ttccacagga 33600tgtgccaaac ctttttctgg tccaaaagat ttttatggtc attctccagt tattagaagg 33660tataataatg attctgtcag ttgtgttcat taaaagtttt ataaaatgtc tgccaatgac 33720attttgcacc ctgtaaactg aatacatagc agttcagtga aagcaagtga gagggtaagg 33780ggtgtcaagg cacagtaatt agcaacctga gctggcacca ccatgtggag ttcctgcccc 33840tctctcagga tagcacatgt gtgacctggg acctttggat gtagggttgt atagtgtaga 33900cagcatccac actcaatcca cagaataaat actagtaaag cttaatttct ttttaaaata 33960tgagggtcac tataaataaa tgttctaata ttttgtttct gtgcccacaa agggggtcat 34020cttgtataac cacctggatg tgtcggtact gaaaatcatt aacctagagg agcagtagtt 34080ggcaaatgtt tttacataaa aggcttgata gtaaatattc ttggctttga gagacacatg 34140gttctggggc agtcacagct gcttgatgca gctgcttgga tcttgaaggc agccatagac 34200aacatgtaaa caaatggata tggtggtgtc ctgggaaaac cttactgatg gacctgaaat 34260ttgaatatca cataattttc acatgtcaca aaatattatt tttcttttga acttttaaaa 34320attttattat ttatttttta gagatgaggg tctcattccg tccactaggc tggaatgcag 34380tggctgtgat catggcttac tgcagccttg aactcctagg ctcaagcgat cctttcccct 34440tagcctccca agtagctagg actacaggtt catgccactg cacctggcta atttttaatt 34500taattaatta attaattttt tttttgagac ggagtctcgc tctgttgccc aggctggagt 34560tcagtggcgc gatcttggct cactgcaagc tccacctgct gggttcacgc tattctcctg 34620cctcagcctc ccgagtagct gggactacag gcgcccacca ccatgcccgg ctaatttttt 34680gtattttttt tagtagagat ggggtttcac catgttagcc aggatggtct cgatctcctg 34740accttgtgat ccgcccgcct cggcctccca aagtgctggg attacaggtg tgaaccactg 34800tgcccagact aattttattt tttaattttt ttcaattaaa tttttagaga tgggggctca 34860ctatattgcc caggctagtc ttgaactcct ggccttaagt gatcctccta cctcggcctc 34920ccaaagtgct gggattacag gcatgagcta ctgtgcctgg cccaattttc ttttgatttt 34980taacaatgta aaaaccattt tagcttgctg cccatttcaa cacaggctgc agtctggaga 35040ctggatttac ctgttggtgg gagtttgcca aaccctaacc tagaggatga agcctaaatt 35100cctgagcatg gtgtttgagg gactggcttt cgaatatcct gcttcagcct acctcccctg 35160gtgttcagcc atgctgggct cctcgatgtt tccctgttct tcacaagata caggtcatcc 35220ttaggcctat gtgcctcact gatgggatgc cctgctgtgg agcatgtgga atgtgggatt 35280tcatatcttt gtgattggtg gattcctatt tatttgtcca tcctggactt cagcgtaaca 35340aatggatatg gtggtgtccc gtgaaaacct ggggggatgg ctttttcttc tgcttgccag 35400gcaggattca atcccttcgt ctcctgggtt gtcacagcac catgtgtgcc tctggtagaa 35460ctttgatttc attgcgaggt aattcttggt gcaccgtatg gtttcatgca gaaaaactag 35520agttgccttt cttgaggatt aggccaagtt cttactcatt tgtgcatcac agtgcctgat 35580acttaggtgc tcagtaggag ttaatttaat gaattcgcaa ttgaggatat gagtcaggag 35640gaattcagga gattggggtt acgaaacttc taagagagaa ttttgttgtc cattccaaag 35700ctggcattct tcctttgcaa gagctctctc ttgtgatgtg gaaaggaaaa tgttttctct 35760ttccctttct gtagctcatc tttccttctg gctcctacct gctctcccct ccccacactc 35820ccagacacac actcacctgc acctgtgtgc tagatctgcc ctgctttcca tcagaaccca 35880gtgcgtcctc cttctgtctt ctggagtctc tcagttgaca gtttttcgtg tgttcctaag 35940gacaggaaat gagccaactc attgaatctg aagttttcaa ctttacatct tagcagtgcc 36000ttgaacattg ccttttacat tataaatagt aagagattaa gattgtctca gaatcacttc 36060ctaactgatt catagggtgg ctttatatct cttttaatgg ggtgtggacc aaaatgcatg 36120tctgggagta tagaaataat cactggaacg aatgtcatac ttacgtgtcg gatataatgg 36180aaagctatta caatacagtt ctcagtcttg tgtcccaaga ctgcattatg agtaaggccc 36240tgggagacac cagaacaagt ctcataattc cttttacttg ttttcttttt gcctgctgat 36300tagaagagga cttcatttga gtagttaatg gctttttcta ttaatatcag cagtatctag 36360atggagtgag aggatagatg aagagatggg tcagtcaaag taaaggaaat taagggaagc 36420aggaagaccc agagaaatta gaataagaga gaaaatctat caaagaaact ctgggtatcg 36480aggaaacatc agctatatac atttaatcaa attcccaaag taagaacata tacttttata 36540attaatggaa tggaataaaa cactaaaagg gccatggaga gagttgactt aggaatcact 36600attctggaat tttaaaagat ttttgatcaa ctgtcttgtc caactttaag attttatact 36660ataattttgc tagaaattct catttgtaga tcttttgaat atctgtactt tatttatttt 36720attttccaat agtctggtac ctcccaagtt ctttgtgact tatttaaatg taatgatttg 36780gtgcatgtgt taaccagttc ccttaataat ctaggatgtg tagcattggg tcccaatgac 36840tttttttttt tttttaacct atttgtttaa atttcctcca gcatacatcc aaacttttca 36900ttgaggaatg ggataaacta cctattttcc ttaaatttgt tttcttttgt tagggggttg 36960aggtgcttac tgtaatttga gtaagtttta atatattttt caagttttat tcctactttc 37020aatttttttt ttccagaata ttcgactttt tactgctttt ctgtgtgcat ttcctgaagg 37080ataattaatc tgcgtacgcc tcctggaata tttttgtctt tacctttgct aattggggga 37140cccagttaag ccctgcttca tctctgatat gttccttggc caccttagcc ctggattcct 37200ctaaattcct ctagtactga tttttcacac ccatcatttt ccatttgcta tgtgctatgt 37260tttattatgt ttcacctgtt caatgtgagt gtccagctcc ttgaccatag ggactgttct 37320gagtagccct tacatgctag atatgatgcc cacgacatta cacgttaccc tatgaattct 37380gatggtattc aggcggtagg gtggttcatg tgtaaagcag acgtctcttc cgttctctcc 37440cattgtggaa gaaagagttg cagagatagg ctttctgatt tctctctggg atgttcactg 37500gtgaaaacca ttgtcagtgg cgctatttgt ttaaaaacac ttttaaatct atcttctctt 37560tgtgcttaaa ttgtttttta aggaccacta agcctgtttt tcacaatggg ctttacgttt 37620tcctggggac cacgctgttg tgattcaggg actccccggt gcttatgtcc agtgggctct 37680tagtaactgt aaactaagca cactaatgga ataaaagtga agtccaggga ttttgataag 37740acttagctgt taatatcagt atttttttca gagatgtcac tagtcacaat gaaaatttat 37800tctaggagtt gccaacatta ggtctctgtt cagataactt ccgagtatgg gttcacttag 37860ccaccaacat ttgcaccagg caagtccaga ctcatttttt taatcagcta gcttgaaaat 37920tgcatttata ttcatgtatt tagttttttc tagtgggtat tgcagttcat aacttttgct 37980taacagagaa gacccgtctc tggcctcatt cctgtataac tcacacatcg cttcaccatg

38040attgttgtta ccagcttact gaagcacctc agtacatctc cggtggacaa tacattagta 38100ttctgttttc ctcatgtact ggttttctcc aattaatatt agacacaaat aataaatctc 38160ttgaacatgc agtgctaaca tacccctttc ctctttagga gctgtttggt gcttgcttac 38220agcataaact gcttagtgtc attccaagct gacacaaatt ggccttcccc tgctgtcctc 38280catgtaccag ttgtatttaa gatgtcaggg gaagctggca tagtggctca cgcctgtaat 38340cccagcaggt tgggaggccg aggtgggtgg attccttgag gccaggagtt tgagactatc 38400ctggccaaca tagtgaaacc ttttctctac taagaataca aaaaattacc tgggcatggt 38460ggcgtgcacc tgtaatccca gctacttggg aggctgaggc acgagaattg cttgaacccg 38520ggaggtggag gttgcagtga gctgagattg tgccatgcac tccagcctgg gcatcttttt 38580tttttttttt aaaataaaaa agatgtcagg ggaataagga ccttgcagag taatgttaga 38640ttgctggatt acctatcaga gtttcagaag ctattcttgt ctttgaaggt gtaaaaatat 38700actctataag ttcttatgga gttatctagc attcctctat agatgatcag ggtctacagg 38760acaagctcat actaagcaaa ctcagaatgt cagaatgtta acagagtaac ttgaagtaag 38820aaacataatc tcaaagggac cagaattctc ttattggctg ctcagaatac ctgctcttct 38880ccatgcagca ggccagtgga ttcctgtagt ttttttctcc tttctctatg ccgtgcagat 38940catggctctg catttggtgg tggttgcttt gttgcttaag tttcagattt taaagctata 39000ctgaagtaca gactcaactt cattctgaat ttagcttccc tcagtaatac atttgtgtct 39060tctcatacac agaattactt tcttggacaa ctaccttaat gtgttgaatg ctgtatttac 39120tggctcggca cagtggctca cacctgtaat cccagcactt tgggaggctg agaggggcgg 39180gacacttgat tttgggaatt tgagaccagc ctggccaaca tagtgaaacc ccgtctctac 39240taaaaataca aaaattagct gggcgtggtg gtgagtgcat ggaatcccaa ttacttggga 39300ggctgaggca tgaggattgt ttgaaccggg gaggtagagg ttgcagtgag ccgagatcat 39360gccactgcac tccagcctcg gcgacagagc aaggccctgt ctcaaaaaat aaaataaaat 39420aaaataaata attaaataaa tgctgtattt actatctttt atttcagagg gtgatctttt 39480tcttattttg gcctgaaatt gttggattgt cccacagtag gctcctctat accatcctac 39540cctgtattct ataaactaga tcacgggctg ttccctgagt atattccttt tccccagtgg 39600ttttgttttc tcctaatgct ctttcctctg tctagattgt tgaaatcctg acacttcatt 39660aagtttcatc ttgaatgcta cttcttcttg actcctcaaa cctgatgtgc tctttctcct 39720ttgaattcta tgcccctttt gtggcactat cttattccac cttatataaa agttacttgt 39780tccatcttcc ctactaatgc acttgaggga ccgtgcatca ctccttggct ctcttctgcg 39840gccgtggacc tgctgctctt agtggtcttc agctatttca gtggccagaa agtcgatcac 39900tgaaactatg acttgcaagc tttcatctgc ttcatctatt tttaggattt ctagttttaa 39960tgggctgttt atgaataaat aatttagctt cttctaggac atgccagtta atgaaacctc 40020agttaatggc tttgagtact aagtgctggt gaaaagtcat ttcttatggt aatacttttg 40080aaattgacca ctattatagt tatggcaaat gtccagggaa gagtgggcat aagaggttgt 40140ggtggtacta ccagcctgtc tctgtagaga tgttatcttg tcacttggct tgtgaagact 40200actcctctag cctccataag ttttgtgtaa agattaaagt gtgtgtagaa aaatctgggt 40260tcttatcaca caacaaggaa agattaggct cacagatact ttgaagggtg aaggggaaca 40320gaatttattg ggtgaaaagg aaaaaactca gcaaagcgag aagggttcct gctaacaggc 40380ccccatctca cagattgaat cccaggttgc cacacaggga caggagaggc caaggtcctc 40440ccctctgcaa acagcgggca cttcctgagg cccaacccca tcctcccagc acgcaggcca 40500gctggagatt ctccaggaag ccgtttttac ttggctgtct cattagtagg agaagtttgc 40560atttttagaa cttgattatt acctggagtt tatttttatt catatacatc agtagttttt 40620taagttggga tgtgtgtact gcatatatat tagaacttct atattttaac ctaattttaa 40680attccaactt tttgtatatc ttttataaag acatattagt acagtggtat atgtatatac 40740tttataaatg catgtgctaa cattgggcat gcttgttcag aattttaagt gacagctgtg 40800tacaaccaga aaagttgaaa ccattgctga atacactagg taaatttcat agacaagtat 40860gtgtgttatt agaaggtttt ggtaagcaag gacatatttt agttctttag tttgctaatg 40920agcttacctg aatttcagtt taaagagtaa aacagaatac atttattaac attttttgta 40980cctatcaata gcttcttgtt tttccccagc agtgtcagtg tattccaagt aatgtctttt 41040ctgtccttaa gcccttaacc ggtcctttgt gtaaagcatg gacactgctt tatgggcttt 41100cagtttgtgt gtttgtttgt ttttaatagc ttcaggggcc ctgtcaaagt taacccgtgg 41160attgaaagat gaatcgctgg cttatatcta tcattgccaa aatcattatt tttgtccaat 41220tggcttcgaa gcaacccctg ttaaagctaa taaagcattc aggtaagcat tgacgtgttt 41280tagaaagtgc attttaagaa atattaaaaa atagatgggt gcggtagctc acgcctgtaa 41340tcctagcact ttgggaggcc gaggtgggcg gatcacgagg tcagaagatc gagaccatcc 41400tggctaacac ggtgaaaccc cgtctctact aaaaatacaa aaaaaaaaaa aaaaaaaaaa 41460ttagccgggc gtggtggcag gcgcctgtag tcccagctac tcgggaggct gagggaggag 41520aatggcgtga acccgggagg cagaacttgc aaaaaaaaaa ttaaaaaaat taaaaaatat 41580tcaaagtctg tgatattggg aggcttggcc atctgctttc ctgacatcaa gttagactat 41640tctttaaaca ttatgactta ttcttctgca gaattgcatt tagttaattg tgctgttgaa 41700aatatccatt tagatactgt tgttcagtca ttataggaaa aagtcatttg aaaagtcact 41760tgtttttctc ttagagacag ggtctcactc tgtcatccag gctggagtgc aatggtgtaa 41820ttataggtca ctgctgcctc aaactcctgg cctcaagcaa tccttctacc tcagcctcct 41880gagtggctag aactacaggc atgcaccacc atgcccagct cataaaattt tttaatttgt 41940gttttctata gagacggggt cttgctatgt tgtgcaggct ggtctcaaac tcctggcctc 42000aagtggtcct tattcctggg cctcccaaag tgctgggatt acaggtgtga gccaccacac 42060ccttcctgaa aagtaatttt tacatttatt ataaaacagt tgcaaaggat tttatagact 42120atgtcacagt gcctcatcca acgtcccaca tgaacaatat attacagaat ctagtgtcaa 42180gaagattgtc acaactgagt aaattaaaaa gcatcttctt ttcttaaagt gaagaatatg 42240gaatttgtct gggttccccc tttgtcctga ttgcaaggaa gctctgagcc aatttttatt 42300tccaatgtca gaaactacat gactttcatg gttatatttc ttttttgcct aatctagagt 42360ttctgtttta ttaactatat aactttactg gtaaacttgc atctttgtgg aagagatgat 42420ccctaagaaa gagaaggtga ctaagtttta atggtttttt tcctgtttag ccacttttcc 42480ttatgatggg ttgagataat accaggatgg ttgaaagcca agggagaagt atctagttaa 42540gaattggtgg gtggggtgca gtggtgtaat cccagcattt tgggaggctg aggcaggcag 42600attgcttgca cccaggagtt caagaccagc ctgggcaaca tagtgagacc ctgtctctta 42660aaaaaataca aaagttagct gagtgtgttg gtatgcacct gtagtcccag ctacttggga 42720ggctgagacg ggaggatcgc ctgagctgag aacatgccac tgcactccag cctgggcaac 42780agagcgagat cctttctgta aaaaaagaaa gaaagaaaga aagaattggt gaaaacacaa 42840gccatgagta ctttgagcaa agggattggc ttggtcatgg ggataggatg ggtttttttt 42900tttttttttt gagacaaggc cttgctgtgt tgcccagact ggagtgcagt ggtgagatca 42960tagctcactg cagcctcgac ctcctgggct taagtgatcc tcccacctca gcctcctgag 43020tagctggaac tacaggtaca caccaccata cttggctaat tttgagatgg ggtttcaggg 43080tttcaccatg ttacccaggc tggtcttgaa ctcctgggct ccagtgatct gaccatgtca 43140gtctcccaaa gtgcttggat tatagacgtg agccaccaca cccagccagg atgggttatt 43200tggggctgga ggccatgtgg ctttggagtc ccactcaaag gcccctcggg tttggggaag 43260agagggattc tgagacaggt tggaatgacc tgggagaacc aggaagctgg aagttgtgtt 43320ttgtgaataa ggagcaagag ggaactctct tgttctctcc caatgctgaa atgctttatt 43380catctttctg gaggtggaat ttcttgtaag tgaggtatac tgtatgtttc ttttatgatt 43440tcatcctgca gcttcacctt atttctgaca tttatcaaat ataaatcatg gttttaaacc 43500aactttgtac cctttaaaag agagtccctc acttctgcct gtacctaaga atcatgtgag 43560attttcttaa aaaactatat aaatccccag gctctactcc agacctattt gaattagaat 43620gtccagggat gggtttagga catcagtgtg tttaaagact tcattgcttt agggtttcat 43680ttttaggatg cgttttgaga tgtcttgaat tcatagtgaa aggagaagag tagtggaaaa 43740atgcagatag cattccagga tttttttttc ctcctagaat tttagaggtg gtatattaaa 43800acattttata gccctagatt ttgaatagag gattaaggct gaaattttaa tttgtgagtt 43860ctctttaatg gcctgtagaa atttgccctc acctaagctc taatgtctgt tcttgatgaa 43920tgaatgccag gtttgaatac actctagtat gccttttatt aatatatatt tgaagcaata 43980tactcaaggg agtaactatt aaacgtactg tgaatgtatt ttatatttag caggggacct 44040ctctcaccac aggaagttga atattggatc ttaattggag aatcaagtag aaaacatcct 44100gccattcact gtaaaaagta tgttaacttc cctttatttt ctttaattga ggtaacattt 44160agatacagtg aaatgcacag atcttagttg tatataatta gtttgataaa tgaatgcacc 44220tgtataatca ccacccaaac aggatatggt acacattcat tgccatggaa aatactctca 44280ctctctactc ctatcaatct ccatagaaag cccctcttct gatttctgtc actacagatt 44340tgctttgcct cttcttgaat tccatgtaaa gagaatcaga caatattatt tttgcatcaa 44400tatcttaagt aacatttttg agattcctcc attgtgtcat gtgtatcaag agtttattat 44460ttttttattg ctgagtagta ttctgttgca taagcatgct gcaatttgtt ttccattttc 44520ctgttgatgg gcatttggat tgctttgagg tttggtctct tgtcaatgaa gctcttggga 44580acattcatgt acaagccttg aaacaaatta tataaaaatt ttattaaaat ataattcaca 44640taccataaag ttcactgcac ccaccatgcg cggtggctca tgcctgtaat cccagcactt 44700tgggaggctg aggtgggagg atcgcttgag cccaggagtt caaggccaac ctgggcaaca 44760tagtgagacc ctgtctctac aaaaaaatta aaaaatgagg caagaggatt gcttgagcct 44820aggagattga ggctgtaata agctgtgatc gtatcgctgc actctcatct gggtgacaga 44880acgagatcct gcctcaaaaa aagggaaaaa agtgtacaat tccatagatt ttattatagt 44940tacagggttc tgcaccaatc accactatat aattgcagaa cattttcatc actccagaaa 45000gaaaccccat accccttggc agtcactccg tattccctga gccctggcaa ccactgatct 45060accttctgtc tctagggatt tgcctattct tgtttgtttg tttgtttggt ttttttaaga 45120cagagtctct ttctgtcact taggtgggag tgcagtggtg tgatgtcagc tcactgaacc 45180ttcatctccc aggttcaaga gattctcctg ccgtagcctc ctgagtagtt gggcttacag 45240gcgcccacta tcacgcccag ctaatttttg tgtttttcgt agagacgggg tttcgccatg 45300ttggccaggc tggtctcaaa ctactgatat caagtgatcc accctcctca gcctcccaaa 45360gtgttgggat tacaggcgtg agccaccacg cctggctggg atttgcctgt tcttgacatt 45420tcgtataaat ggtgtcatac agtatgtagc attttgtacc tggcttcttt cacttaatac 45480agtgttttca atgtcatcca tgttttagca tggattagaa cttcattcct ttatatggcc 45540aaatcatttc ctttgtatgg agaagtcact tttgttgtac aagtctttta gaggacatat 45600gttttgttct cctggaagac acctaggact agaattgctg attcatagta tagatgtata 45660tttaagaaac tgtgagaaaa ttctccaaag tggttgtaaa ccttctgatg aacagaagtt 45720ctgattctaa ttaatcttaa ttaaatttat tttatcaatt ttcttctttt atatgttttg 45780tgcctctata agaaattttt gcctacccca cgacctcaaa gatgctttcc tgtttttttc 45840tagaagcttt atagatttta tgtttagatt tctgatccct ttctattatt gttttgagac 45900agggtctctc tctatcaccc aggccagaga acagcggtgt gatcatggct cactaaagcc 45960tcgacctcct gggttcaagt gatcctccca tctcagtctc ctgagtagct gagaccacag 46020atgtgtgccc ccatgcccta ctaattttaa aacttttttg tagagacaga gtcttgctgt 46080gttgtccagg ctggtttcaa actcctggac tcaagtgatc ttacttcctt ggcctcccaa 46140agggccggga ttacagatgt gagccaccat gcctggcctt aattaccttt tgtgtgtggt 46200atgtggtaga gctcaacttt gattttttcc cccagtgttt atctagttat tctagcacta 46260tttgtttaca aagattttcc tttccgtatt taacttcttt ggtgcctctg ctgaaaactg 46320tatgtgtggg ctgtttctgg accctgtcct actaatctgt ctgtcctttt accgatgcca 46380cactctcaat tgttgtttat actaaatcct gaaattagat agcacgaatt ctctgaattt 46440ttctttttct taaagattgt tttggctctt ctaggacctt ggcttttcca taaaaatttt 46500agaatttgtc cttttaatta gagtttgtaa agattttgat gaggatagaa tctatagaat 46560tcataatttt ggctgggcgc ggtggctcat gcttgtaatc ccagcgctta ggtgggagga 46620tcacttcagc tcaggagttc gagaccagcc ttggcactat ggcgaaaccc catctctact 46680aaaacgacaa aaattagcca ggcatggtgg catgtgcgtg tagtttcagc tactcgggag 46740gctgaggggg gaggatcaac tgagcctggg aggtcgaggc tgcagtgagc tgagattgca 46800ccactgcact cctccctaag tgataaggtg agaccctgtc tcataaagaa agaaagaatt 46860catagtttag ttttggaaga attgacatca taacaacatg gactatatct ttacttaatt 46920agagctttaa tttcaacttt ttaaaaaaat tttttcagta tggagtttta tgtcttttat 46980taaacatttt ttctctatgt gttctattac actgctggtg ggaatgtaaa ttagtacaat 47040cactatgaca aacagtatgg agattcctta aagaactaaa agtagatcta ccatttgatc 47100cagcaatccc actactgagt atctgcccaa aggaaaataa gtcatatgaa aaacacacag 47160gcacacacgt ttatagtggt acaattcgca gttgcaaaga tggagaatca acctaagtgc 47220ccatcaacca atgagtggat aaagaaaatg tggtatgtat attaccacgg aatactactc 47280agccataaaa aggaatgaaa tgatgtcttt tgcaacaact tgggtggagc cggaggccgt 47340tattctaagt gaagtaactc aggaatggaa agccaaatat catatgtttt cactttaagt 47400gggagctaag ctatgcggat gcaaaggcat aagaatgata taatggactt tggggactcc 47460tgagggcagt ctgggaggcg ggtgagagat aaaagactac atattggatt cagtgtacac 47520tgcttggatg atgggtgcac taaaatctca gaaatcacca ccgaagaact tattggtata 47580accaaaaccc agctgtaccc caaaaacaat tgaagtaaaa cttaaaaaag atattgcaat 47640aaaaaggttt tcctcagtgt gttatacttt ttgatgctat tgtaaattga tttttttaaa 47700atttcatttc ttcattattt gctggttgaa tacaaaaata caattgattt tcctatgtag 47760accttgaatc ttccatcttt accaaatcct tttagttcta gtagttgttt tgtaattccc 47820ttggattttc tgcacaattg tgttgtctcc taatagagac atattaactt tacattcttt 47880taaaagtgga cttgactgta attgaaaagg aaatatagca tattgctgct ttagcatgca 47940ggatgtcatc taacatgttg gtatttattt attctgtagg aagagtaacc aattgtacac 48000tgaaaacctt ggcaacggtt ggataaacag aattgtcata aaactagaag ttattacagt 48060ttagtagatg aaacaatgtt gagataagag aattactgcc aattattgct gtgataatat 48120tgcagcaact tgctcatgaa aaattccatt gcaatgatac tttagaccta aagaaaattg 48180tgctttatcc tttcttctta actatagatg ggcagatatt gttactgatc taaacactca 48240aaatccagaa tacctggata tccggcactt agagagggga ctgcagtata gaaaaacaaa 48300gaaggtaaga agaacaccat tgtgtttgaa ggcatttccc agctgaccaa aatgtggtgt 48360tttacttagc actctttagg ttgcaagtaa cagaaaccta tatttgatag aggagtgaaa 48420attattttaa ggacacacgg ctgtctttaa aacccaaggg caaggggtat aacctggagt 48480cataaaggaa tggtagatgg tagcaggtat tggaatgaca gcaagggcca agtagctggt 48540agtctgcttc actcttttgg gggctatata ttgttcttag ctttcctttt gtctgattac 48600tttcttcgta agatcatcat ctttccattt atgtgtcgtt tttagatgaa cagtctagac 48660tgagactgac ctgtcttggg ttcagttccg tagtcttagc ccagcttggg ccagattaac 48720tgtggccttg tggcagagtc atgtgtacaa acaaggcttt gggagaggct ctcaggaaat 48780gatatcttat tgatgcaatc ttactcatat aactatatga agaataggag agagaggaaa 48840aggaatgatc ttggtgtttg aattaggcag atggggaagt tctctagtat gtgttgggag 48900tgagtagtag agaggcatgg ggaggaaggt gttttggctc cggtgggcat tgttatattt 48960tgtaaaggca gctgttagaa aaggtgcagg atgaggtgct ctgatgatgc ctggaaggaa 49020agtaccattc tgaggccggt gaagtaaaaa aagaaagaag ggagttcata gggacctaca 49080aattagtaca attaatttcc ctttattttc ttcagccaac atttttttct tctcaggttg 49140ggggaaattt gcattgcatc atagcattcc agagacttaa ctggcaaaga tttggccttt 49200ggaactttcc atttggaacc attagacaag aatcacaacc tccaacacat gcccagggaa 49260ttgccaaatc tgagagtgaa gacaatattt ccaagaagca gcatgggcgt ctgggccggt 49320ctttcagtgc tagtttccat caggactcgg catggaaaaa gatgtctagt atccatgaga 49380gaaggaacag tggttaccag ggttacagtg attacgatgg gaatgattga ctatgcttgc 49440tactgaacag ctggcattat atatgaaact gctatataca ggactgtata aagacagtag 49500aagattttag taagcctaca ttaaatagga gcagatcttg tggtataaaa aataaccttg 49560tagttctcca gatactaagc ttgtatatga ttatggtggg tgatttcaga tatataagca 49620gataagcaca gattattgtc ctttcaagtt aagagtatat aatctggaca gaaaatttca 49680caaaattcaa taaaattaca actgttgtct aaataagtga aacacaaatt cacttaatag 49740catcaagatt tgaaatactt aagcatgaag tgacttttat aatgactcga tccctagaca 49800tttgttacag atagttttat gcctaagacc aagatgtaaa gtaccatctg cccttaaaaa 49860aaattggggc tgtcaatttc tagttttcac tcatggttaa cacgcattta aaattatttc 49920atgagtctag tagttctttg atttatagca ggatcttgct tgcctcattt gtttcctggt 49980tatgttctta ggattctgac taagaggcaa aagagaaaag actcaagaaa ctgatcctgg 50040agatcgagac catcctggct aacatggtga aaccccgtct ctactaaaca tacaaaaaat 50100tagccgggtg tagtggtggg cacctgtagt cctagctact cgagaggctg aggcaggaga 50160atggcgtgaa cccgggaggt ggagcttgca gtgagcggag atcgcgccac tgcactccag 50220cctgggcgac agggcaagac tctgtctcaa aaaaaaaaaa aaaaaaaaag acggatcctt 50280ttttttggtg caaatgggtg acttagtgca ttgattcaga tttttaaaat ttcttgatgt 50340ggtttgtaat aatcaaatat tgacaagaac cttaggtctc gaaagacttt tataagtcta 50400gatgacgttt gccttagggg taaagtaaaa gaacaattgg caccttaagt ttctataccc 50460aaggttatct gtgaaatgag atctcctgat atttgattgc tttctcagta tggagtcata 50520tgttgataac agtactgaag atgcataaga aatgcccaag tcactcagag gacaactacc 50580catattccag actctgagct gtttcctttt taaaaatcat atagacaatt agctgtttga 50640agtgagtatt aaatatttca gaagtgtgaa tttcatgtat ttgagctcct ctagttgctg 50700ttggtttttc ttctgctgcc aacctgtgac tcacaaatga ctaggatctc ttgttcttta 50760attttagggt cttgttccag gactcaaatc agtaacttgg tgattacaag gtgctgaatg 50820tgttggtaac catatcgcaa tacacctcaa ggaaaaggtt cagattttta tttttaaaat 50880attttcattt ttttcttgaa ttttatatcc gtttgttcac tcgtacatgc ctagcctaca 50940gaaggggata tatattatga aatggtcatt tttctgaaga gaatattttg cttgaaatgc 51000aaaggactga aagagatttg taggttgttg attttgttac ttcatactgg aacttttaaa 51060aagatttcat caaataaagt tttgttttct acttttaatt atatgaatgt ttttaaacct 51120ttgttttagg tagaaggtac cattgtgtct ttgaagtaca tgataatttg tcaattctgc 51180tcaactggta cattgtaaga aaccatcttg agccttttat aattaatgaa acaatatgca 51240ttatgatgac tgtaatttta gatttctaat ttaataggaa atagaaaatt tgattctttt 51300attagcttga accaaatgaa gttgtcatct ttgtaggtca aaaatggttg aatattagca 51360atttcataca gttcaactga atatttcaga gaagacgtag gtaggaagaa ccaagaggaa 51420aggagaaaaa tgcaaaataa aataagaaat taaaggatag ggagacacct agaaaaacag 51480gatgagttat tcagactatt gaccataatc aatttttata aaagtcttga tctgttctaa 51540gtttggcttc caagtttggc tttccagtta ttaagagcac aatgaggttt gagtttagtg 51600agattatctt tcccgcagaa gctgtaagca agagttactg catacttctc ttagaagatt 51660agtaaaattc ccttgatatt tgatttcttg gtcagctctt aggaatccta tagatacagt 51720gaaagttcaa atactggctc tgcacttaca agctatatca cctatagtga tataggcaag 51780ttaaggttaa ttttttttct gtgcctattt cctcattagt aaagtggggg taatagtatc 51840tactttataa atgaatttga agaataagct aatacatgta gtgtttagaa cagtgctttg 51900tagataggaa gtgctattta agagcttgct attattccaa aagatgtgaa ttttactatt 51960cagagtcttt agagagagcc ctttagatag catcttaagg agctaattcc ttttaaatca 52020catatgcacc ccttagttgc tgtttcttca aaagaatatt tcatattcaa gaatgttgct 52080ttattttttt gagacagagt ctcgctctat tgccaaggct ggagtgcagt ggtgctctct 52140gcaacctccg cctcccgggt tcaagtggtt ctcctgcttc agcctcccga gtagctggga 52200ttacaggcat gcaccaccat gcccggctaa tttttgtatt tttagtagag actgggtttc 52260accatgttgg ccagggtggt ctcgagctcc cggcctcaag tgatctgcct gcctcggcct 52320cccaaagtgc tgggattaca ggcgtgagcc accatgccca gcctttgctt tggttttaaa 52380agtacttaca atactcaaat gcctatgttg gctatttatt tttacccagc tcacaggcag 52440aaaaaaaaaa gtttatttag atcaaattct gcagcaattc ctttccctac tgctattact 52500gttaaagaac tgtgtgccat cattaggcca agttggtgca gcacccaaaa tacttgccaa 52560cttgtcttct cccagactgg aggggctcag gcagctcttc taggatccat cacattctgc 52620atctcaactt taccaataac actcccaccc ctcctgtacc agcgattcac tcaacaaact 52680aaattataca ccattaccag gtcagttctt aaatagctca gcagcaacaa gaacaacaag 52740atgttggtcg cattctaatt ttactggcag aaactgaggg tttcattggt gaagaaacct 52800gcctaggatc acagtgtctt agtttctcag ggctaccata acaaaacacc acagacaggg 52860tggcgtaaac aacagaaatt tgttttctca tggttctgga aactagaagt ccaggagcag 52920gcaggtttgg tttcttctga ggcctgtctc cttcacctgc agagggccgc cttctcactg 52980tgtcctcaca aggcctttcc tctgtgccta tcatccctgg agtctctccc tgtggcccaa 53040tttcctcttt ctacaaagac accagtcaaa ttggatttag ggcctatcct aatggcctcg

53100tttcaattta atcattttta aacgctgtgt cttcagatag tcacattctg aggtactcag 53160gcttccacag atgaattttg gaggacacaa ttcagaccat aacacaaggc aacatatggt 53220tcagatgaga tctgaatgaa tgatcagcct aacctccaag cagattcttt cagcagactg 53280caaggtgcac tggagagctt tagactagag gcttaagagg tcatttaggc aatatttaca 53340gaactgctaa gtgccaggat tgggggatgt agcagtcacg aaaatcgttc ttcgtttcag 53400taagtttaca gtttaccatg gggaagagac aaatactgaa caggcagtta tattactctc 53460agtaaaataa caactgggaa cagtttctgg ggattacttt acatatggag gaaatgcaaa 53520aaacactttg tcaggattat tcctgtagca aattagatgt gactaggtca accaaacatg 53580gccactgtga accatcttat tgagcataga agtggttttg ctaaaaatgg atttctccac 53640ggagcacggt ggctcatgtc tgtaactcca gcactttggg agactgaggc aggaggattg 53700tttgagacca gtttgggcaa caaagcaaga ccctgtctca aaaaaaaaaa aatcagggcg 53760tggtggtggg ggcctgtaat ctcaactact cgggaggctg aggcaagagc atcacttgag 53820cctgagaggt ggaagttgca gtaagctgag actgcactac tgcactctag cctgggcgac 53880agagtgagac cctgtcaaaa aaataaaaaa taaaaaaata aaaaaaatca atttcccgta 53940tacttctgtt ataaagaagt ctttaaaaaa ttgcctgctc ccttaagtca gccttttcac 54000tgagtttaaa tttcgttcca atttgaacaa atatggatgc taatactatc ctttatagtt 54060actagtgcta agtgcgttgc atgtaacacc ttgaatactt actgaagtct gcaaggttgg 54120ttttatgccc tgattactga tggagacaca aattctgaac aactgtgact tcagggatgc 54180taaacaccat agtgaggcac gatgcgggga tttgaatctt ggcctgaggg ttccagagct 54240gtggcctttt ctggggttac tctgttaatt gatttctagt cctttctgat ctacaagccg 54300cggcattata acttttagat gctgaagaaa actaaactat atgtcaagga ttaaggcttg 54360tgaaccccca aaatttggga caggtctcag ttaatttaga aagtttattt tgccaacgtt 54420aaggacgcgc agctgtgaca cagccccagg aagtccagat gacatgtgcc caaggtggtt 54480ggggcacaga ttggttttat acattttagg gagacaggag acatcaatca acatatgtaa 54540gtacactggt tccttccaga aaggtgggga caactcggaa gcaggaaggg cttctaggtc 54600acaggtagat gagagacaaa aggctgcata cgagtttctg ataagccttt ccaaaggaga 54660caatcagaat atgcatctat ctcagtgagc agaaggatga ctgactagaa tgggaggcag 54720gttttgccct gagcagttcc cagcttgact tttccctttt gcttagtaat tttgggaccc 54780taacattttc acaggcttta aattttatta ttctttagtt actacgtgct agcatataaa 54840taaatagtac aaaaccaaga aggcatccac cttttggttg tctcttcacg tgtaaaacaa 54900cactttgtgt taagtatctt cacacacggc ggcgcaaagg tagaaaccga tactaaaaaa 54960gcgtgtagaa aatagttccc agcctgggca acacagggag acctcatttc tacaaaaata 55020attcgccaag catagtggtg cgcacctgcg gtcccagcta cttgagaggc tgagatggga 55080aagttgcttg agctcgggca gcaggagttc caggctgcag tgagctaaga atgcgccact 55140ggactccagt atgggcgaca gcgtgagacc ctgtctcaaa caaaaacaaa agcccgttac 55200tccaccaaga aggcgctttt gcacattgtt ttaatgctta acgccttcag gatgccagcg 55260tgacggaagc aagtaaccac caaggcatca ccactggcgc taaacttctc acttccggag 55320tgctgcaagc gcagaaaata tacgtcatgt gcggaggcgg agcttccgcc ctgcgcgtcg 55380tattagacgg aaaccgagcg ggcccatttt tcatgggttt gcggacccac cagcgaaggc 55440gggaggtgtc gcagggacat cttctggctg tttccgtcgc ctgcgtggcc cttgcacccc 55500ggtcttccat tagcggcgca gacgtttggg cctaagcgct gggcgaggcg aggccctgcc 55560cctccccgcc aacggccatt ctctggacct gtctttcttc cgggaggcgg tgacagctgc 55620tgagacgtgt tgcagccaga gtctctccgc tttaatgcgc tcccattagt gccgtccccc 55680actggaaaac cgtggcttct gtattatttg ccatctttgt tgtgtaggag cagggagggc 55740ttcctcccgg ggtcctaggc ggcggtgcag tccgtcgtag aagaattaga gtagaagttg 55800tcggggtccg ctcttaggac gcagccgcct catgggggtc caggggctct ggaagctgct 55860ggagtgctcc gggcggcagg tcagccccga agcgctggaa gggaagatcc tggctgttgg 55920tatccttaac gccgcgttgg gacttggggt gcagggattc ggggctggat tcctcgcggg 55980gctctgcctt gggcacagtg gcatctgcag gatgatggtc ttgggtcggg gtcggggtcg 56040ctatagaatc tctgtcacta ggttttctaa gtacagtcgt ccctcggtat ccccggggct 56100ttggttccag cccctcctcc gtataccacg atgttcaagt tccttcaact cccttatata 56160atggcgtggt atttgcatat aaactaccca cttccgtaat cttttaaatc gtttctagct 56220tacttgtaat gccgaatgca atgtaagtga tctgtaaata gttgttatac tgtattttaa 56280aattttttgt agtttttatt ggtatgtttt atttatttat ttttttccat cgcaaatatt 56340tttgatccgt ggtaggttga ttgcggaatc gggtgatgag gcgggccgcc ctgtctgctt 56400tccccagctt tgcagtctta gcggcctgtg catcctggtt tgtcactttg tggcagtgct 56460ttatgttcct ctctgcctta gttctctcat ctgcaaagta gaggtggtga tagtatctac 56520acacaggatt ggaatgagga ataaagaaat tgcctacatg agagaagttt agtgcgatta 56580atacagtaaa tcttaaagtt attatcctgt ccgggaggtc agtaaggaga gcagagtaga 56640cttcgacgat tagttttgct tgagtcttgc cccatttatg tttcttagag gaaggatagt 56700gtggacaggt gttttaccca ttttttaaat tgacttttta aggactattg tttctgtaca 56760tgtttggctg gttttgtttg tcattggaat taaattcttt tttcattagc aaaacgtgat 56820actgcttttg aaatttttat ctttttcttt gttatgtagt catttttttt ttttttttga 56880gtatctattc tggctcagaa tctggtgata ccttacacca atttcctaaa tggggatcta 56940tgagtctagg ggctcgtggg cttgtggaaa gagtcctgta aatgattgcg gggggattgg 57000ggacgggaaa tggagtgcaa gagtgtgtgg gcgttcagca aaggaatccc tgactgtgga 57060gccctaattc ttcagtaggt acaaaactta taaataaaac gacattgata agttttaaaa 57120cataatgaga ttatcatttt ttgcaagtga ataatcttta aataaatctg taattgattt 57180ttggcttaaa agtttttata cagggtactt tgaattgggg aaagccaaag gatttttgtt 57240ttgttttgtt ttgttttttg ttttttagag cacatgcgct gttgctgccc tcaagctgtt 57300gcttagtatc attgcagtct aaagattttc tcagaaataa agggtaaagg ttagttttca 57360gtgacaagaa cccttaaaac ttcagcaaag atttagatca ttttatgtag cagcccttgt 57420gaagaattac taaagaggac tgtggccagg ctcagtggct tacgcctgtc atcccagcac 57480tttgggaggt caaggcagga ggatcatttg agcccaggag ttcaagacca gcctgggcaa 57540catagtgaac ccctgtctac acacagtcaa aaaattagct ggacgtggtg gcctgcacct 57600ttggtcccag ctacacggaa ggctgaggca ggaagattgc ttgggcccag gcggtcaagg 57660ctgcaatgag ccgtgttcat gtctgtctct gaataaataa ataaaaagga ctgtgaaaga 57720cattctagtc atgacaacct catatattta taataaactc gtttatgtca aaggagtata 57780tctgattact tgttctacaa tcggttttgc tttatttcat tttcacggga attcagacac 57840ctgaaattat ttagatttta ttgtattgtt tgttaatgac aaacttaccc gttattatac 57900tagtttcata atttactctt gtatctctat actaaattgt ttggtcttta aaccatctat 57960atagcttagt gtcttattta tacagtctaa atgtctgtgt caaataatgc agaagtaagt 58020taaacttttg tctcttatag attttaatgt ttgtaatatg cgtttaagtg tattagcaag 58080atattaccat ctctttaggc tatgtatact aatggagatt tttaagtcac ctaactaaag 58140aactaaagaa acttattttt tgtattgaaa tgttattggt ctttgggtct tataccgaaa 58200gtgatttttg gctttgctgc agattttaac ttttctgtcc gcagaaattt aattttgcgt 58260atataaactt actgaaatta gacaagtcaa attatacaaa tattttcaga ttgtctcatt 58320tttcatattt cttgtctaac aatttatgtg aatattttca ggtcgtctaa ttttacatat 58380ttcatctcta agaattcaag ccaaaattct caaccctagc tgagaattgg cattacctgt 58440agttaattga aaaataaaaa ataaaagaag tcctacctca gtctctatga tttagaattt 58500taggcccatg gggtttaata atccgtatgt ttcagaagct tcatatccta ttctaatgga 58560tagcagggat gagagccact gatctgaaac tagatttcct cactgaagtt aaagtttaaa 58620gtagtgggcc aagtagaaac taatttaatc ctatgtaata gtgagcttca tggcttttcc 58680agactatact tgtagcaagc agaaacatcg atctaataca agacagatgc acatcaacac 58740caagttctac ctctgagatg tatcttttat ccgatctctt ctcgtggtct ccaccactac 58800tgtctgcgtt aacttgggct attgtaactc attttagaac tggtttcctt ataatctgtt 58860cttcacatgt tcatcaaagt tatccttaaa tcatgttcta gaaacttccc agtggccgta 58920tcacctcctg ccattccttt tgtcctgctg cactggcttc ctggagatag ggaccgtctc 58980tgttttgttc atggctgaat tgtttggtgt atatccctag ggtcttggat atgcctggcc 59040catggtattg ctaaatatta tagaaataag catagcagta gcttgctttc cactaggtat 59100tttgttacat agtgttttgt aaattaattg ttgatggacc tacatttttt tttatgttag 59160taaaagttag gcagtgtaca ttcatttaaa tactaaactg ttcaacttat ttaatagcag 59220atatttattg tgtataagag tccctgtgaa agggagtgca gtgcacaaat tgcccgggca 59280agccatggag cacacaggct gggttttttc cctgctagat tataaactcc atagggccaa 59340tactgtgttt tgttcagtgc actggatatg ccagtgcata gaaaaaatgc tgccacataa 59400taaaaacaga aataacgtga taggtggccc cttcaatgtt cttttcctat gttagaaatt 59460tacctgcaaa tattagcatc agtccaggtt tctctctgtt ttttttttct ttttgttttt 59520ttttgagaca gagtctccct ctgttacgca ggctcgagtg cagtggcgtg atcttggctc 59580actgcaacct tcgcctccca ggttcaatcg attctcctgc ctcagcctta taggcacacg 59640ccaccatgcc tggctaattt ttgtattttt agtagagaca ggatttcacc attttggcca 59700ggatggtctc gatctcctga cctcaggtgg tctgcccgcc ttggcctccc aaagtgctgg 59760gattacaagt gtgagctacc gcactcggcc tcaatatatt ttttaaatag gaatgtatac 59820aaggaggatg tcttcatcta gactgaggag gggacccctg gactgagacc ttagagtagg 59880agagaactag gcggagagag ggagaaactg tttcaggcag aggaagcagc atgtgagaag 59940aacctggagg caggaaggag cttagtgatt tctgggtttg aggaaagccc agtagcagga 60000agtgagcaga ggccagatca ttcaaggctt cctagaccag gagaagaagt tttagatagt 60060atcttgaagg cagtgggaag ctttggaggg ttttcaccaa agatacgttg gtgaaatcta 60120gtatatacct tcaagataca ttagaagata gaattgatag acctggtaat agctataggt 60180ggtgatgccc agcattctgg ctcaggtgaa tggcagggac attcactgag atgggggcat 60240gagcagctga gtggagaaga catggagttc agttttgatc atgttaagtt tgaaatgtct 60300atgaggtatg gggtagagac gtcatctggg ctctctgttc tggggtcagg agcgtgctct 60360gggccagaca tatacatgga gtcagggcgt ggtgtatgtg gaggggagtg gaggttgata 60420gttcagacag gaggaaagaa gagggcctga gaaagatcac tgatgaagtg aatactttaa 60480cagtgttaca ggagctggca aagagtctga gagcaggaaa accagcagag cgcggtgtca 60540ctctttcttt tggacaaata taacattttt tgagaagcac tttttagaaa agtttaaaaa 60600tataagaaaa cttcaagatg aaaatataag ttatctttag tctcagcaga cataatatct 60660actttaatat tttggtatgt gaactagttt tttatattta acatatatac tgtatcagaa 60720aatggagtta tactttatat ggttttatag cctgttttac tatctttaat aatatttaat 60780gactatcttc cttatcacac atgctcctgc aacttccatt tttaagggct ttttggtatt 60840ccagcaaata ggtctgccat aattagtttt tcttttttga gacggagtct tgctctgtca 60900ccaggctgga gtgcagtggc gcgatctcgg ctcactgcaa cctctgcctc ctgggttcaa 60960gcgattctcc tgcctcagcc tcctgagtag ctgggactac aggtgcgcac caccatgccc 61020agccaatttt tgtattttta gtagagatgg ggtttcacca tgttggccag gatggtctcg 61080atctcttgat ctcgtgatcc acctgcctcg gcctcccaaa gtgctgggat tacaggtgtg 61140agccaccgcg cccggcctat gattagtttt ttaaaaagac ctgctggaaa acttttaagt 61200tgatttttat attttcatta ttataaacaa gcattctgta aataactctc attatatatc 61260catgatgatt ttctgaggat caatgtctgg aagtggtaaa atagactgca aagttttaaa 61320aagttttttt cactatgaat tgtcaaattg tcctccagaa aatttgtgct aattcatgaa 61380aattctttag caatcactta ttttttttta aagaagcaat tctttgcaat gagacttctt 61440aaaaggatat gtctgtcttt gtatgatttt aaaatgcagt atgtgaatag gggtaacaag 61500agttcaacta aaagttaact gactttaggt agatcccatg agagctaaat gtttttcaat 61560tttaaatgaa tagtgataag tatttagtgt tcaacgtttg gataatatca gttattagga 61620aattgaagtt gtgaggatga agagaaaaat cccggagttt tttccattaa caattctccc 61680agatattagc atttggttaa accaagcact taaaggagtc cgggatcgcc atgggaactc 61740aatagaaaat cctcatcttc tcactttgtt tcatcggctc tgcaaactct tattttttcg 61800aattcgtcct atttttgtgt ttgatgggga tgctccacta ttgaagaaac agactttggt 61860aagtgtcgta tagtttttag taagtgtcaa ataatttttt tctttctgca ttcttagaaa 61920aattcacata aaatttttgt tttctcttta gaattttaga aacagactta ttttgacaca 61980tacttaatta catctacttt tttatcttga acaattaatt tttcttttaa aaagttttat 62040gagagttcat catgggtaca atgataaaat ttaactttta aataaaacta actactaaaa 62100accttgctgt tgagagtttt cccttcagag gatcttgtta ggtgttttat atttatttca 62160aggatgcggc catcactcag aacactgtgg aaaccctctt ttgggaagtg cctccctgaa 62220tctgagatca agtatcctcc gtgatagcaa gccttctttt aaggggaatt ggattttagg 62280agtaagcaat gttatgtggt gccaagtggt gactaaagtg gattatctag gaaggaaatt 62340gaccatgata tagaagagtc agaatgagtt gctgctttat tgttctggaa gtagttttaa 62400aaaatgtttt tggaaggccg ggggcaggag gattgcttga gcccaagtcc agcctgggca 62460acacagggag accccatctc taaaaaaatg acataaaata atgtttaaac agtccaaatg 62520acagcaccca tttggatata tgacttttat ttgttgaaaa taccaaagta atgaaatgat 62580gactttgtaa tcataccaga tactctctaa atgaggatat aggataaaag taaggatata 62640ggataaaaat aaaaagcatt tataaatgct gggggacctt tgttgcctca gtaagagtgt 62700ctagtcttga atgttgcagt tttcagttac ccaaggcata ctgtaaatca ggtactttgt 62760ggaaggatta gaaaggaaaa gagaatatct tggttatttt tatccgatat tcagccataa 62820gcacagggac tatccatttg tcactacttg aacatttctt acaagatatt tctatacatt 62880ttagaatttc tcatgatata taatcttgaa tattgaaggg cagaattaca tgtaagtaat 62940gcatgtttat aaaaagcaat attagaaatt gtttttggtg tcactgtgac attttataga 63000ataaataaat aatttctttt gggaagcatt taagctttcc acttcttggg catattaaaa 63060gacatttaaa agaaactgaa agtaaattaa gtttcccaaa tatattgtgt agtagcccat 63120taagtaatag atggaaactc tgggtgtcct ttacgtagca gcaacctgaa gatatacact 63180gatatggcaa ttaggaggaa atgctaaagc agccatagtc tgaaaactca gacaaaccaa 63240attctctgga aaataaatca cagcaatgtt tctagtggtc taatatcctg aagtgagatc 63300ttacatcctt tcttctcata ggtgaagaga aggcagagaa aggacttagc gtccagtgac 63360tccaggaaaa cgacagagaa gcttctgaaa acatttttga aaagacaagc catcaaaact 63420gccttcagaa gcaaaaggca agaggaaaat tatagtcgtg ttagagatga agttttaaaa 63480aagtgatttt tgtcttgatt tcctgcgatt ctctttccct atctaatttt gactctcaac 63540agaaaataga gagtgaaatg agacaagtag gctgccattt tgacctggta atttggagtt 63600gtggcaattc tccgttctgt gagaatcaac tttgctaatg agaaaaaaaa gctgtcgtgt 63660tgcgtcatgt acacttttta ctttgattat ggtcttcttg actctaggtg agcagccccg 63720ccaaggttcc ttcctttctc tcggctgcat ttattttcca cagcagtggc ctgagagcag 63780ccaggtcagg tccctgttca ccatcctgag cagggtctgc ataatctgtt taaagatttg 63840tgtactttcc agagatgaag cactacccag tcttacccaa gttcgaagag aaaacgacct 63900ctatgttttg cctcctttac aagaggaaga aaaacacagg taaatgttta actatttaag 63960aatattattt tagtcattgc tacattcaga cacatttaaa ccttgatgtg ttatctacat 64020gataaggcat gtgaacattt cttaatgcat ctgaaatagg catgctctat acctttcaga 64080atatttttca aagactaaat tttttattta ctattctttg tgttttggtg attcatgatt 64140cttattcctc ttcattctaa agaacttctg ccagggctgt ggattctaaa ttctccaagg 64200catgaatttt agttttgctt acactttgct tacacatgct gtctctagct gcttttcaat 64260ccagtatggt ctctctcacc atgatgctaa aataccacaa cccatgacat tcttgttgct 64320aaatctagca gacgctttgc acgtcttagt gtgctcgact tctctgcact aaacaccatg 64380ccacactgcc ccgctccccc ggcatctctc tggcgctttc tgctcagcct ctctgtggac 64440tcctcttcct ctgtctctct cttaatggtt ggtggtttct gtgtttgaag tgaaggtgga 64500gaaccggaag cttggcagtt tgcctttgtc ccctgtcctt ccctccacct cttcctttcc 64560caggagttcc caagagacaa cttagctttt gtatcagctc agttctattt gtctccattg 64620ctaccactgt cattttttct ccaggttaca ttattagctt ttaaacttct ttctgtcttt 64680gtctttgatc ataccctttt tgtaatagtt cctcccacca agcccaatct ctgttttgtg 64740ttatagtctg tttaaaatcc aaatctgata ttgtcatttc cttacttaaa acactttcta 64800gcttctcctt ctcatttttt tctttctttt ttttttgaga tggagtctct gttgcccaga 64860ctggagtgca gtggcacaat ctcagctcac tgcaacccct gcctcctggg tttaagcgat 64920tctcctgcct cagcctccgg agtagctggg attacaggca cgtgccacca cacctggcta 64980attttttgta tttttagtag agacgaggtt tcaccatgtt gtccaggctg gtctcgaact 65040cctgacctca agtgatccac ctgcctcggc ctcccaaagt gctgggattg caggcgtgag 65100ccaccacacg cagcttagct tctcatttct ttaggataaa actttaactc cttaattaca 65160ctttccagtc ttatccctgc ctttcttcac atcatccaca ggcaggcttt cttttccttg 65220tttctatatg gtcatacaac ttagttcttt tgcctggaac actcttctcc atgaaccttc 65280ccgctcccta caactacact cattaattcg tggctaactc caaccagtgt aattgcagga 65340gatcctggaa agcttttgtg actcctaaat tgggtgaggt gactccctat tgcatctcta 65400gtgacctgcc cttcccttgt ggtggaagtt attagatccc actctaattg cttacttaca 65460cgtgtccccc accagaccat gagaccagta gtggcacaga tcttgctgtt ttattcacca 65520ctgtagcccg tataacgagc agagccttgc atacaagtat ttttgtaagg ggtccttaaa 65580aatcatagat atcgtaaaag tatgtttgac tttcagttca gaagaggaag atgaaaaaga 65640atggcaagaa agaatgaatc aaaaacaagc attacaggta tttagatcat ttttgaattc 65700agaatgtatt ctgttatttg aaatgaatga catgaaaatg aatattaatg aggtatatca 65760aactgtgaaa gttcctgata aaaagtaaag acagatggct ttttggttgt gcatatatat 65820gtgtacatgt atgtatttaa aacacactca cctacacacg tgtatatata tatatatgga 65880atttgccatt atgcacatct atatattcaa aacaagctat ttttctttta caaccaacca 65940accaatagta atatgtgctt acatagaaaa tatagaaaac atatcattca taaataactt 66000gtaataacag tttgatatat tttcatccat tctttgcctg ccccatatag ttgaatatct 66060gtgtgtgtat ttgtatatat gtatcaaatg tgcatgtgta catatatatg tgtacacata 66120tgtgtgtatg ttctagttat ctgtcgctgt gtaacttagt ggttaaaaca tcatttgtta 66180tcgtcatctg tctgggttcc aggggtagat gagctcagac aggcagttct ctctggggtc 66240tcttgtgtag atgcagccag tctgtagctg gggctggagt gattaacgag gtttccttgc 66300tgccatgggc ggtggttgat gctggctgtc agccagtctt ccatgcggcc agcaccagag 66360cacctacttg tggcctctcc acgtggcctg ggcattttcc cagcatgatg actgtattcc 66420cagtgtgggc atcccaagag aaagttctaa gcagaagccc tgttgccttt ctgacctagc 66480tttggaagtt gtgcagcatc cttcccacta catcctgttc ctcagaagct agttactgcc 66540ctatggaagg ggcagagagt tagacaagaa gaatgtccca cacatatttt catgaataac 66600atttttacaa attgagatta tgctgtatat atacattttt ttcataaaca tttttcaagt 66660ttcttagtgt cacagatagc attttttgat tcccaaattc actgtttctt gtttgttcat 66720atggttttgt tctatcaggt agaaactttt gagctaaaac ctcgagatcc ttcaggttgc 66780ggaggtgctg tgctattaat gcagctagtg agagcaatct tagttaagac catgggcctc 66840gtggtcaaaa agacctgcgt ttcagtccag gctccactgt tcactagctg tgtgatctta 66900cacagattaa attctccaag gctttatttt tttttgtaaa acgaaattga tatcaatgcc 66960ttcctgatgg gggtgatggg agcattagtg agggaaagca caggaaatgc tgagcgcagt 67020gacaggtctg tcctacgtgt ttgctggttg gtacgtacac atgtaacata cagacatgca 67080ggacatcagg ttgtattagc ttatcttttt tactaatgaa ggattttaaa gtacttttgg 67140taatgagtta aagttgagaa aagttttaca cttttccatg tcttctgtga atttctttta 67200atttcatttc ttatgttata agcaaaataa aactaactta attatgggaa tgatatatat 67260tgcttgaatt cctaaggcac attctgtctt gggttcttgc attttctgtt ctctcagcct 67320gaaatgctct atcctcagtg aggctctttg atatcttcca agtccctgct cacacatcct 67380ctttttagtg gggtcttctt tatctatcaa atagatcttt ttttccacgt tctgttcatg 67440taatcactct catatcagat aaatctgctt tattttacca ttatcttgtt catttattta 67500tttatttatt gtcttctctc ctctgctcct aagagcaggg accttatctg gcaccttatc 67560cgatctcaca gtaccttagc acctagaaca gtgcctggtg ccttagaggt acctgctaag 67620tatttttttc ttttgaatga attaaagagt gaatggctac attccctaat ttgcctactc 67680actttgttgc ctgtcacaga ttatatgcaa ctgtgtttag ccaattgttg attatgtaga 67740actgtgttta ttatataaac ataatacata tccttaatgt tgaatagaac taagtgtatg 67800aaatgtaaat ttcatggtgc tgtgatttta tctttacagg aagagttctt tcataatcct 67860caagcgatag atattgagtc tgaggacttc agcagcctgc cccctgaagt aaagcatgaa 67920atcttgactg atatgaaaga gttcaccaag cgcagaagaa cattatttga agcaatgcca 67980gaggtgaaat atgcaacagt acattcatgc ttagaattaa gaacttcagc aaaacttttt 68040attagaaaga agagaaaatt gataagcaat acttacacga tatctcagtt aacagtaaac 68100agcatttcta catctcagat tctaagaagc atcgtatatt tatacgtttg agcctataga

68160catttactct aagaagtttt tcttgacttt tgacccgaga ctaggtcttt tttcctggtc 68220tttgttctca cagcaccctg taatatcact tcatagttct tagttccaaa acacgcttat 68280cttgctcacc tctgtatttt cagtgtctag ctcagtattt ttcacatggt atgtgtccag 68340tagatgctta ctgactcaat tcttaggtta ggtcataaaa gttattgtaa cctataatat 68400acattgtcta taaaaactaa tagtcatata gaatctaatc acaatggaaa aataagttct 68460aaattgaaat tccaggtata tcttcctctg ctgcagccct agagatgcca ttggctctcc 68520acattccctt gccctcttcc tggacagtgc gaatggggct tcttcacctt ggaacatctt 68580gtagcttggc aggcccagaa agctagagtg gaggtggtat gtgcagttgg gtgctagcaa 68640atgtgtctcc tgatcatgct gccattgata cttaattcat gttactattg atgactccct 68700gtcttagttg ccagtgagtg agttcttttt ctctttctgt tgctgctacc tgttatttct 68760accgtagttc tccattcacc cactatagga cagaatcgaa attttgcagc atcatcgacc 68820ttagtgcata gatggagtgt ttttttattt tctacaattt ttgaatattg cttaaattga 68880tagcagaaat atgaaaaagg aagggtaaat ttcttttctc atcactccct gttttttcca 68940caaagaatgt gcagtagcac acactaaggt gcacagaagt gacattcttg ggtctttgga 69000tatacaaagg acagaagtaa attgattttt atttcaggag aaaaatccag gctcagtctg 69060tctatcaggc attttatttc ttgagtatga aaggatctct ggctggcagt tgaggaagta 69120gaattttggt tgtgtaaaca ataacaggaa gaaatgggag aaagagagac agtccctaat 69180gatttactgt tctttatttt ctttctgcaa ccatgaagtc tctggaagtg gtggactgta 69240gggtggtgtg gagtagcagc ttactggatc tgtaattttg atagagatgt tctaagtcat 69300ccatgttggg cctttgtgtg atctgtatgt cgtgcaaatg taatattgat aatagtagtg 69360atggtaggta ataatagcag tagtaataat cataatacca tagttccact ttactcacgg 69420tttgcagttt tcagtgacct gtggtaaact gtggtctgaa aatattaaat ggaaactttc 69480agaaataaac aattcataag ttttaagttg cacaccattc tgagtagagt gatgaaatct 69540cacaccctcc tgctccatct tgcctggaac gtgaatcctc cctttgtcta gcatctccgt 69600gctgtagatg cttcctgcct gttaatcact gagtagctgt cgcggtgatc agatcaactg 69660tcgcgatatt gcagtgcttc tcctcaagtc actcttattt gacttaatga tggcacaaca 69720gtgcaagagt atgatgctgg caatttgaat atgccaaaga gaagctgtaa agtgcctcct 69780ttaaatgaaa aggtgaaagt tcttgaatta ataaggaaag aaaaaaatcg tattctggct 69840gaggttgctt agatctgcat aaaaatgact tttctatctg tgatattgtg aagaagcgaa 69900aagaaattgg tgctagtttt gctgccgtac cataaactgc aaaagtcatg acctcagcgt 69960gtgataagtg ctcagttagg atggaaaagc cattacattt tggggtggaa gacatgaaga 70020gaaacatgtt ctgattgatg acaatcaggt ttggtacttc tgcagtttca ggcatcctct 70080gggggtcttg gaacataccc caaggatgag ggggctgtct actatgttaa tagaatcaat 70140tgtagtaaat tgacatgctt ttgatcccag atctaccact tattagccct gtgactctag 70200ggaggttacc taacctattt aagtcccaat ttcttcattt ataaaatgga ggtgatatct 70260gtttcatagg atgattgtga gaataaaatg aggtattata tgtaaaagca cttagaaaaa 70320tgccctccat gggaaatgcc ttataatgtt aagtattact gttaataact gtgattactg 70380tgatttattg tgtcttttat gggataaggt tgtgcaggac acttcacttg catatttacc 70440tacattctag aagattgtta agccataatc agatgtcata gtgactgcta tgcattacat 70500gctcaataca tgtttattga ataatgatta aatcataaac agtattcatg attttttttt 70560tttttttttt gaggggaagt ctcgctcttg tccctcaggc tggagtgcaa tggcacaatc 70620tcggctcact gcaacctctg cctccccggt tcaagtgatt ctcctgcctc agtctcctgg 70680gtagctggga ttacaggtgc ctgccatcac acccggctaa tttttgtatt tttagtagag 70740acagggtttc accacgttgg ccaggctggt ctcgaactcc tgacctccgg tggtccaccc 70800atctcggcct cccaaagtgc taggattaca ggcatgagcc accgcacctg gccatagtat 70860tcatgatttt tttttgccca actctttcga agattatttt tttaaaagga agctgtagtt 70920tttcttgtta ttcacctttt ataatatgaa actaccatca atgaaaaaag ccaattgttc 70980tttgttccct gttggggaaa gggtggaaat atggtaatat tatctgtatt taatataaaa 71040cagtaatttt gtttgtttat tttgccttta ggagtctgat gacttttcac agtaccaact 71100caaaggcttg cttaaaaaga actatctgaa ccagcatata gaacatgtcc aaaaggaaat 71160gaatcagcaa cattcaggac acatccgaag gcagtatgaa gatgaagggg gctttctgaa 71220ggaggtagag tcaaggagag tggtctctga agacacttca cattacatct tgataaaagg 71280tatcaggcac catcatttat atatttacat taaaaaatca aagatatatc atgactctga 71340attctataaa ctagcacccc tggataatat taatgaaatt ctatttatgt aataactgta 71400tactgctatt aatggattaa ctactatagt gccaaaccac tttaaaatta gctaatgaat 71460taactcctag ttgccgatta aatgaaaatg tatatactta tttatgagaa ccagtgttct 71520cttatccatc ttactagaag cgtattgtca cactgtaaaa ctgaatggtg agaagtgttt 71580taattcttct taaggtattc aagctaagac agttgcagaa gtggattcag agtctcttcc 71640ttcttccagc aaaatgcacg gcatgtcttt tgacgtgaag tcatctccat gtgaaaaact 71700gaagacagag aaagagcctg atgctacccc tccttctcca agaactttac tagctatgca 71760agctgccctg ctgggaagta gctcagaaga ggagctggag agtgaaaatc gaaggcaggc 71820ccgtgggagg aacgcacctg ctgctgtaga cgaaggctcc atatcacccc ggactctttc 71880agccattaag agagctcttg acgatgacga agatgtaaaa gtgtgtgctg gggatgatgt 71940gcagacggga gggccaggag cagaagaaat gcgtataaac agctccaccg agaacagtga 72000tgaaggactt aaagtgagag atggaaaagg aataccgttt actgcaacac ttgcgtcatc 72060tagtgtgaac tctgcagagg agcacgtagc cagcactaat gaggggagag agcccacaga 72120ctcagttcca aaagaacaaa tgtcacttgt tcacgtgggg actgaagcct ttccgataag 72180tgatgagtct atgattaagg acagaaaaga tcggctgcct ctggagagtg cagtggttag 72240acatagtgac gcacctgggc tcccgaatgg aagggaactg acaccggcat ctccaacttg 72300tacaaattct gtgtcaaaga atgaaacaca tgctgaagtg cttgagcagc agaacgaact 72360ttgcccatat gagagtaaat tcgattcttc tcttctttca agtgatgatg aaacaaaatg 72420taaaccgaat tctgcttctg aagtcattgg ccctgtcagt ttgcaagaaa caagtagcat 72480agtaagtgtc ccttcagagg cagtagataa tgtggaaaat gtggtgtcat ttaatgctaa 72540agagcatgag aattttctgg aaaccatcca agaacagcag accactgaat ctgcaggcca 72600ggatttaatt tccattccaa aggccgtgga accaatggaa attgactcgg aagaaagtga 72660atctgatggt acgtgtctgt gcttttgtag aaatctggaa cggtaggatt tcccctctgt 72720aggaattcag agatcggtta gtgtagtccc gttttaactt tttacagata aggaacgaga 72780gacgtagaaa gaaagatgaa atgactttcc cagggagtca cagctggtca tggaatcttg 72840accttccctg tgttgctctg ctttttgtta tcatttttaa aggcatgaag tgccctattt 72900ggggaaggta aagttgagtt tccctctagt ttttaaaaac tttttatttt gaaataatta 72960tgaactttaa aaagttggaa gaatattata aaacactggt tccttcatcc agtacctcag 73020tggctagcat gttaccacat tagcttagaa tttctcttgg tctctctgtg gccctctata 73080tgtatatcat atatctccaa atctgtatac atatgtatac cattgatcct cattatttgt 73140agattccata tttgcaaaat tgcctgatca ctaaaattta tttataactc caaaatcagt 73200actcaccgca atgtctttgt ggtcatttgt ggacatttgc agagttgggg aaaagcttga 73260gttgccacac tgtcccctgc tgaggttaag caaggtgaca ctctgcctgg ttcccgtgtt 73320ctgagagaga tgaccagagg gtggggacag taggggatta tgcaatggag agagagcaag 73380aagctccggc cccaggccag ttggaccaga tttgaatccc tattctggca cctgttagcg 73440tggcagcttc acacaggtca ctaatttgtt tcttgaactt tgtttcttgt ttataaaata 73500aatggaatct attaagatgg tggtttttta ggatttaaga taatatatat gaaatgtgtt 73560catatatatg ttatatatgc atatgtgtgt atatgcacat agatatgttt aggagcaatg 73620actcggtatt ggctaattta gtgttcacag agacttcata cgtgatggcc actttgaata 73680agagaatcaa ccacacacac acacacacac acacacacac acacacacac aatttgttcc 73740tggtatctgc tagttttctt cattcaaatg ttactatttc ccttttgtaa ttaataagta 73800ttttgtggaa aaggaatttt tggagctata taaatatgct gttcctgaac aaacttccac 73860ccacttgtta gcatccattg atgtttacct gaataatttg ttactacgtt ggttgccaaa 73920tgatggtttt tctaactcca tcattcctta tatattatta cttgacatcc tcctatgagg 73980aagatctttt ccttctcccc atttattttt attattttta atcagtgtag actcctgtat 74040tcctatttag tgagttataa tccaatactg tcataattta ctttgttact caaattatca 74100cagctttggc cattggggct ccttctaatg gctttcagca gttttttcat tatattttga 74160gcttttcctt gctttctggc caagctgttt caggcatatg ttgtactttc tctgccctgg 74220tcctggaacc agccatttca ccagggagct ctggttcctt tcagtggagc atggggttta 74280gacaccacaa gctggatgtg agtgtgctta tggatcctga ggtataactg tctcaggcct 74340tttcagcaac agtgccagga agtatattta tgtatacata tacatgcaca cacacatcta 74400tatttatttc tatgtctatc tgtactaaaa tccatgagtt tatactgaca tctgcaattc 74460catggggttc agtctagcct cctgcttctt tatagtttcc ctaacaatga gaaacattgc 74520tccccttatc ctcaatacat ttacatctgc ttattctccc tggatatgta accatctccc 74580cctcccactg gcctcctcct tggccctgct ctcttctttg cttcagctgt gtccttggtg 74640ccagctccca gtccctgaga gccccctcct ctgttctgat tgtctcctta aacccagctg 74700gacaggcctt gccagccctc tccacctaca gggaaggaag gcaaccatta aatatatttt 74760aaggagaagg aaagacagta agacagtaag agaggagaga agggaagtgg aagaggaaga 74820actatttctt agtcacagct ttattctgtg ctgtgtaaat agcataaaaa catactgagc 74880aacttccatg attgtttata tactttgata atcctccttt ttgaattttt aaaacaatgt 74940cagttaactt agaacatatt tatataaagc gaatatacaa atcttaagag agtttcctac 75000tttcaaagac agtgccagtt tacctaattg aaaaggcttg ttttgaagtt acaggcattt 75060gtgattacat ttatttatta ataacgctac tattacatgt attctgttat agtcatatct 75120ttccttttta ggatgtagca tttttcaggt tcctccagaa agctcttgat gattgcagga 75180tcattttaat gttttgattg tagatgaagt gaccttttaa ttttggtaca ggaagtttca 75240ttgaagtgca aagtgtgatt agtgatgagg aacttcaagc agaattccct gaaacttcca 75300aacctccctc agaacaaggc gaagaggaac tggtaggaac tagggaggga gaagcccctg 75360ctgagtccga gagcctcctg agggacaact ctgagaggga cgacgtggat ggtgagccac 75420aggaagctga gaaagatgcg gaagattcgc tccatgaatg gcaagatatt aatttggtaa 75480taccgtaaca ttgtgtttcg acttcttgct gaggaagcca ggttaagtag gttttgagtt 75540ttaaggagtt ggtggatgag tatttagtag ctatttgcag tacatcttgt ggttgctgat 75600ggcttcattt ttgtgtaggt tactggctgg gatagactcc gttttccatg tggtttagtg 75660atgaatctct aaagatatta cagagtcttg gttagacatc cagtggagta cttcctaagg 75720agaaagagct tattggtaat ttcagtcaga ctaaatgcag gctttttgta aacaaaactc 75780atttggatta ttaatataaa tctataaatg aaaaaacatt ttataggagg agttggaaac 75840tctggagagc aacctcttag cacagcagaa ttcactgaaa gctcaaaaac agcagcaaga 75900acggatcgct gctactgtca ccggacagat gttcctggaa agccaggtgg gtgcaggcag 75960cttgggtttc ctttaccacc ttcttcagac ccctggggga atgcactgca tgaagggggt 76020atgcactgtg ccccctggtg ctcagggcct ggtgatgccg ttccctgggg gtcactgtgt 76080gtccctaact ctgcaggaat gaatgcatta catgaagtgg taggcactgc tccccctgtg 76140ctcagggcct ggcggtgccc ttccctgggc gtcactgtgt acccctcact ctgcaggaac 76200tcctgcgcct gttcggcatt ccctacatcc aggctcccat ggaagcagag gcgcagtgcg 76260ccatcctgga cctgactgat cagacttccg gaaccatcac tgatgacagt gatatctggc 76320tgtttggagc gcggcatgtc tatagaaact tttttaataa aaacaagttt gtagaatatt 76380atcaatatgt ggactttcac aatcaattgg gtaagacttc agagtctttt tgattacttt 76440ctgacattta ccttcagagt ttgtcctagg aagttttctt tccaaggaac tagtttgatg 76500cattgatgga aattgcaggt ctatgcaaat ttttatatga gtgatctttg gcttatatag 76560aggaatagga ttttaaacat ttgaattaag gaattaaagt cctagtatgt ttaggtagtt 76620aatcaactga cttagttaaa ctttgactag ttacccgaga tctccacagt gaacaaaagg 76680tggtggagag gggaagcagg ccgcgcctgg gcctgtattc gggtttctgg cactgatctt 76740cttctgttca tccagcaaat atatgtttgg tgactcctat atgccagttg ttcttaacaa 76800gagggaagga acagagagta aaatagtagg agaaacagat gataagcaga tacataaatc 76860ataatttgac agttggtggt aagtgccaaa tagaaaaata taacagtaaa ggagaggaga 76920gagtgaactt caggcatcga gagtgcaggt gctgtttcag agagttgttg ggaaaggctt 76980tactcgtgta acttcaaggc agggacctgc aggaaatagg aagcaagcac tgtagataga 77040tacctcggga ctagtgagtg aaaggggctg aggcagaagc ttgtgggctg tgttctaggg 77100aagcaaggag gccagtgtga gaggaggagg gaggaaatgg caagggttaa tcttagagaa 77160taggaagaag ctgaaaccca ccagggtctg gtgtgctagg gtatgcaggc agattgaatg 77220tggggtggga gaagaatgaa gtcgaggagg attccagata ctgagcagct ggtcgagtgg 77280acctgccttc agtaagacaa ggagggagca cagtggagga ggagaaacga agtgttcagg 77340tttggatatg ataaattggg gatgctcatt ggatacccag tggaggtgtt gagttgatag 77400ttgaatatat gtgactgtgg ttcagagaga ctcaggctag atagctaact ttgggggtca 77460gcgtatagag ggtatttaaa gctatgagaa tggatgaaac tttaaaaata ttaacagaat 77520gccattgaat aaaataattt attttcaaat aataagatat ttttggtggt tggatataga 77580tatagatata cacacgtaca tgatttatat aataaaatgt ttataaatgt catataagaa 77640atcttgataa aaattaaaaa atattgttac tctttaggat tggaccggaa taagttaata 77700aatttggctt atttgcttgg aagtgattat accgaaggaa taccaactgt gggttgtgta 77760accgccatgg aaattctcaa tgaattccct gggcatggcc tggaacctct cctaaaattc 77820tcgtaaggtc ttttatttct ttaatttgga taattgtgta aatacccaaa taagcaaata 77880gaactattat ttacagcatg aactgtcatg ctgtaacatg tgaacaatgg ttcactgaga 77940aagcagcaga aagtattggt tgttttccat tttctagaga tgatgaaatc agagtcagtt 78000cttagtggtg ctgggcttat cctagttcaa gggtacaaag ccagtcctgt ggatttcaca 78060ggaatgtaga agttgccttt tcatccattg acatacttat agagcagcta tgatgtgtca 78120gacactgtgc tggccctggg gaaaggagag atgagtaagg cataggccat accctcaagg 78180actcccccca tatacttgga ggtgatggag aagcaaagca aattgtactt gtctgtgtgt 78240ttggtgtcct gagagaggtc agccaggaag cgacttggaa atataatgcc aggaatgtta 78300catccagcac tcctgtcctg tttctcacca tgtgaatccc gtgacgtgtt cagtggtgaa 78360gtcttgctcc atccagaacc cagagtcgtg ctatactcgg ggtacattta tcattttgaa 78420gattctgaga tggtaggagt tatgagtatg tctgggaaag tagttttttc tttgtccttg 78480atggcatctt tttaaaaaat tgaaatatag tcacatacca cagagttcac cgatttaaag 78540tgtacagttc attggttttt agtgtattca caagattgtg gtggcatctt ttaaccgtca 78600ctatgtcgtg agatgctgtt tggtggtggc tgatttaatg atccttgagt gttctctcca 78660gttacatatc ctgtgtttga aagatgctag gatgctctga cctgaagagt cagtgactgg 78720aatcactgaa atgaacggcg aaactgttgt agtgatcatg gtcttccagt catagcacac 78780tcaccaatgt gttgtatttt atactttctt catttatctt tttctgattt tcttattgtt 78840gaggaaattt atataccggg aatgagttat gaggtagaca tacgtcatgg taggtaaata 78900acttgaaaac ctacaaataa ctttattata caaataaagt aaatacatca tagaaaatta 78960taaatatgta ttaaaaagat gattacagtt tacaatttgt ttatgtaatt ttagacttca 79020cacatatata tgcatataca ttgcatatat atgtatactc atttgagtgt gtgtatgatt 79080ttttttataa gaatgggatg atgctatata ctttttattt tacctgtaac ccatttggta 79140ttagaacaag acctattatc aacgatgata tagatttata tcgtttttaa tggctacagg 79200tagtatcttt ttgcgtctat cagggctagt ttaatcaagt gtcttttgtt atttctattt 79260ttagaaaggt attaacagac attgatttga gtgtccttaa tcttcgggtt cttatctaat 79320gattatttta gaataagtga aatagtatct gacttttcca tttggtttct tgataactat 79380tgagagtgaa ttttttatgt tcatagacgg tttgtgtttc tgatttagtg aatgacctgt 79440ttatgctgtt ccacttttct ttgttcagca gtttttgctc gtttgttgcc tgctgaatca 79500ttttcatatt tgagcagttt ctgcctttgg tcctccagct cgttgatgcc agataggagc 79560agccttcgct ctgcctggct caggacattt aggatgaata cagagtaaag agcaggaagg 79620atggtgggaa accagtaaaa tcagaatgtg gcctcacttt gttttctgtt ctcctcttct 79680gagggcaccc tggctggtga atcattctta gagtggggct gcattgggcc tgcaggttga 79740attgagctgt agtcacattt ggtttggact acacggtgta ttcttctctt gagctttaat 79800tttcaattgg ctaccaacat ttacctttgg gagattttac acaaaattat ggattctgtt 79860gggggaaaaa ggagagctga aaatactggg cctgaatttc tggatagccc caaaatagct 79920ggcatggagc aacagcagtt cccctcccct accccaaggc cttggactgg gcatgaatcc 79980ttcagtttca tcacagttgc ctccactccc ccagctcagc tgattgacag acaccttcct 80040cctttcatct ttatgtgaag tgccaggccc tcctgcttga aggagtaacc atagctttgg 80100cttgatgagc tcacaagtgc actgattcta actgccagtc atgattacag tttcaaaaac 80160gaaaatctgc aagtaaagta gaggagaatt atcggtattc tctcagtgca ctcctggaaa 80220gaatgttgta aaagtaatga agaaaaatta ttttttcctt tttgcatttt gttgttataa 80280tactttgaag accgcaatta gtaatttcca acgctagatg gcactcctgc atcggtgagg 80340cggaccctgg ccctgacagc cggtctgagt gggttcctaa tgctgcacta gcaagcttgt 80400gaagcggggg agcctgagct gcgggcgttt tttcaggttc cattgagtac atagattggt 80460gaggattatg ttagtaactt tgatggcatt tattttttcc taattgtgta aatgtttctg 80520gtcagtcgtc actgcattac aaactacctt cagccttagt ggcttcacac agcagtagtc 80580actgattttg ctcatgcctc tgaagctcgc tcaggcctgg tgggggtgtc ttgtctctgt 80640tccatgtcgg ctaggacagc tcagctgggc taggacccct taagatggct cgcggggctg 80700atgaggtggc ttcggcctcg gggcctgggt ggacacttgg tgagcttggc ttcctcatag 80760catgttggag ccaagagcag gaggtccagg ggacaggaag tggcgagggc ctcccctagg 80820cctggcacgg cttgagtcac actggtcagc catggccgcc ctgctcaaga ggaggccaca 80880gggaccccct tcgtgatggg aggagggtcc aagagtgtgt cgctgtcttt atccggccga 80940tttaggctta gaatggaaaa cattgaaaaa tgaataaaat ataaagaaga aaatgaatgg 81000ctgtgtgctc acgtagaata ttgcctagca cacagcgaaa taatagttgt tagtgttatt 81060tattttaagt atctgtttac ttttctcata tatgtgtttt ttccaaaact ggaatcatac 81120tttaagtaga gttctattat ctctgttttt gtatatattt atttgtttta catacatatg 81180tatttatata tatataaaat gttaaatttt aaaaacagat acatgtatct gtttttacag 81240attttttaaa caaatttaac attttcttgg catatttaca ttttattaaa tattcttcga 81300tccattattg tatttgattt gaggtaaata atacagatac attacttcca ttttactgct 81360gagaaagaga cccagagaag ttgtaacttc tgtggtataa ttatgagtgt gggctctaga 81420ggaagactgt accaattcca gctgtgaaat cttgagccag ttacttattt ttgagttcag 81480ttccctcctc tgtgaggtga gggtaacgat agggcatggc ttgtgtgatg attgggcatg 81540tgaaatattt aacacagtgc caagcacaga ggaagacttc cataaatgtt tgccatcatt 81600atacattgtg gctaatagta ctaaaattaa taaaatattc tatatagcat acaatttgag 81660ttagaacttg agtttacatg ttctaagttt agttcttcat ccatagtgta tgaactataa 81720tgtctcattg ctgtgtaagt aattgtttcc tttattttac agagaatggt ggcatgaagc 81780tcaaaaaaat ccaaagataa gacctaatcc tcatgacacc aaagtgaaaa aaaaattacg 81840gacattgcaa ctcacccctg gctttcctaa cccagctgtt gccgaggcct acctcaaacc 81900cgtggtggat gactcgaagg gatcctttct gtgggggaaa cctgatctcg acaaaattag 81960agaatatcct ttgcttctta aaagagaagg aaacaccttg tcaaatatgt gtttggttta 82020aaacttatgg aagagaaact tggatcattt ttttcttata tcccgttaat cccattttct 82080taatatcccg ctctcctttt cactctttct tccctctcct cagttgttgt tttttgtttg 82140tttgtttgtt ttaaaggaac agtgtatcac tctgtcaccc agcctggagt gcagtggtgc 82200aatcatagct cactgcagcc accacttcct gggctcaagg aatcctcctg cctcagcctc 82260ctaagtagca caagccactg catctggccc ctctccttag ttttaaagag ggtctttgtt 82320tgaataagca gtgataagca ttcatagata taaccacata cttaaatatt attgattctg 82380aaagaaggaa agatgactaa tcttgtcctt tttattactg gttgtccttt ccgtgtatat 82440ttaagtaatc atttggatag atgtttcaaa acttgcacta gaattaatcc aactacgttt 82500gattttttct cttaatgaaa taggcaagat atctgaaact tgtttttatt ccagttaacc 82560ttttttcata gtcacaagtc tttgatgtcc taaagtgaga gctaaatatg ttttatttta 82620tagaattgaa aagaacatag tgccagatga ttatgctgga gtcatttatt ttgttagtac 82680tttcttttag cactctaatc tttataaata ggaaaatctt aggagataca gggaatggaa 82740tcaagaatgg gttctttgga cctttttatt tgtcacttgt ttaaatatct ttcaaaatat 82800ttataagtct taactgcatg catattttgt cagcggtatt tcggctggaa cagaacgaag 82860acagatgaat ctctgtttcc tgtattaaag caactcgatg cccagcaggt aatcatggtg 82920gacccttctc ctaagttcag gatgaagggt aggctgtggt tgacagctgt taaagaccag 82980ttaactctta ttttggggca tagagcagac attttgaaat taggataatt tagatgagag 83040aatagaagaa aatagaaaaa gaaaagttag actttggctt cattttctat gatcactctt 83100agggctgaac tttggagtcc tttcatattc atttttttct ttgaaaaaac cagtctaata 83160actgatttca cactaaggtg tttctgatta aatacattac cccttgggat ttactttcat

83220tttttaacga aaggaagtct tcaaagcaat tctgatcatt taagttttta taagtactaa 83280tatttttaaa ttctataaac gaatcttgaa aggtagaagt tcagtcatta ttgtgtatca 83340gtaggagagg tttttgtggg aaggagccct tttgtatgac ctttagttcc tctaggtacc 83400tacttcttgc tttatctgtg agtatgttct gactcacaca aaacaagctt tcgcccacac 83460tagaaacaac ccacaaaaca tacatacaaa taaaccactt agcagaaatg agaatgtagc 83520ttttgtgata atatatttaa attaaaccca ttttcattag gcttggataa ttttgaaaaa 83580catgaacaaa actaagacag catttctatg agtttctctt tgttttttgg atgactatct 83640actggaaaca ttataaacca accaaaaagg aaagctattt gtcttagtgt ctggtgattt 83700cttaatttca gataaatttt gttgtaatat ttatctcctt tttcattcta aaacttaatg 83760tgtttatttt tccaaaatta gaaaatgaag aaaatgaatt aaaactactc attagttacc 83820tcctctaata cagttgttaa catttaggtt aaaaaatttt cctgcattta tttgtggcca 83880tactaaatgt aacttaatag atctaatcct cccccccaac ttattagcat attttcttct 83940atgtattgtg tggttttctt aattccagat gactagatta tatttcatga aagatattta 84000aataattagt tcagtttact tgttagacct ttaattttca agtttgttat agtaatgctg 84060tgatgaacat taggcatata gctttaccca tatatttttt ttttgagacg gagtctcact 84120ctgtctccca ggctggagtg cagtggtgag aactcggctc actgcaagct ccgcctcccg 84180ggttcacgcc attctcctgc ctcagcctcc cgagtagctg ggactacagg cgcccgccac 84240cacgcctggc taatgttttg tattttttta gtagagacgg ggtttcaccg tgtaagccag 84300aatggtctcg atctgacctc gtgatccacc cacctcggcc tcccagagtg ctggggttac 84360aggcgtgagc caccatgccc ggccaaagaa tatcttctta aaagtgaatt tactgaataa 84420aaggcatgaa tatttcttac agttgctaat atatactgtg aacttgcctc tcaaaggtat 84480tgtatgatga taatgttttt aaaagaaaga tatagtagga cttagaaaca ggccccatga 84540agtcgtgttg ctcctgagga agatgaaatg tttagctaca gaaaaatatt taacgctctt 84600tgaatatctt aggaagagat ttctcatttg agatgtggac taaagactta gttgacagag 84660atggtacaag tactgcttta ggtatgattt agaaagtgaa aattactgtc agtaattcac 84720tgggagagaa ctgggttttg ggagataatg aattaatatt ctctgacata gtaatccaat 84780gtgagtgatc aaggttgagc ttgttgattt ggtttagaaa cttgacttac ttgtctgatt 84840tattattatt attcttttgt tattttttta gacacagctc cgaattgatt ccttctttag 84900attagcacaa caggagaaag aagatgctaa acgtattaag agccagagac taaacagagc 84960tgtgacatgt atgctaagga aagagaaaga agcagcagcc agcgaaatag aagcagtttc 85020tgttgccatg gagaaagaat ttgagctact tgataaggca aaaggaaaaa cccagaagag 85080aggcataaca aataccttag aagagtcatc aagcctgaaa agaaagaggc tttcagattc 85140taaaggaaag aatacatgcg gtggattttt gggggagacc tgcctctcag aatcatctga 85200tggatcttca agtgaagatg ctgaaagttc atctttaatg aatgtacaaa ggagaacagc 85260tgcgaaagag ccaaaaacca gtgcttcaga ttcgcagaac tcagtgaagg aagctcccgt 85320gaagaatgga ggtgcgacca ccagcagctc tagtgatagt gatgacgatg gagggaaaga 85380gaagatggtc ctcgtgaccg ccagatctgt gtttgggaag aaaagaagga aactaagacg 85440tgcgagggga agaaaaagga aaacctaatt aaaaaatatg tatcctctat aattagttat 85500gacagccatt tgtaatgaat ttgtcgcaaa gacgtaataa aattaactgg tggcacggtc 85560tttgtattta gtgtgtggtt cctaaaaaca aatgctaaat ctgacatttg ttttttaatg 85620ttttactttt ctagtatttt ttagctgaat atttcaagta tcattggata ttatcttgta 85680ttcacaggct ttgtcttttc atgttttcat tatcttaaca atgtctgatc cttcctggtc 85740acatgttaaa aaagcgaaaa agatttctat tgatcagcac tcactctcaa taggctttcc 85800ctctgacatt cagacgtagc tgagaagaaa tacgtgcatg tttctaattc cacaatagtg 85860gcagttttac acaactgttt agccctgctg cccacggctt tgcattttcc ctcaggttcc 85920acttaaaagc atagcaggag ggagcctcac tgctggaaca tatttcaata tgtttgctgt 85980ggttttagca aacaattagg aaacctaaat ggggtgcatc attctacctg tgaacattaa 86040gtgtatggga acctctgtac cgttatgttt ggcttttaaa ccagacttca cttattagaa 86100gctgacttct gtgtaaatgg atttggaggc tgggggctgg agttgaactg gtgtaggtgg 86160gtcagcttta ggagtggcct gcaggggatg attgttgttg acacagtgtt tgttcagagt 86220ggacaaagaa ggttatttta agactgctcc tgtggagacg tctcccaaga acaaccccca 86280agctcctatt tgcttcgagt taagaatgat tggaggagag tactaccaat tactttgagc 86340gtgggtcctc tgccttcaga gctaccttcc caagtctgtg tccttttggc atctttagta 86400tttccatctt tcctgacttt ttccttcagc cttcaaattg aaaatcttca ccaatgaaaa 86460caaaatccaa atagataatc tccagcagtc tttgaacgac tcctagatgt gttgtttttt 86520aggctctcat ttatattgac ttggccagcc aaacttctcc agtacttgga ctacagcagc 86580tgcttttctt tgcacactat tttttcctaa tttcaaatac gtcttctgca tccatcattc 86640ttctctagca gctccttcta aagtcaacaa tgatttccac ataaactcaa atcttttctt 86700catgcttctt ttctctggag gtacttggag cagaacattt ttggaggcaa ggggagggga 86760aaattaactt tttcgtgttt ttctcatttc tcagaattac ttgtcactgg gagttttctg 86820catatatatt tgcaggtaga ataattgtct tgctaacaaa tttaatttga tgcctttaat 86880agatatttac atttgttttt cactgcgtta gctttgggaa aggataagct actgtaacaa 86940aagatgctca tatacagtgg tctgaataag ctagaacatt acttctctct tactcaacag 87000tctggccagt ctgagctggt gaagcagttg tgcaccatga ggtcatgcag tcctgtgttc 87060agccaacccc tagggtgcta tcttgcctga gtggtctata ctgggtgatt acttcatctg 87120cattgcatcc tgccagaagg ggaaagaacc aaagtccagg gcaagcagaa ggggaaatgg 87180atggtcagga ccatgagaaa cctgttctgt aacactattt atatgagagt ttgatattgt 87240atcttctttt tctcttaacc ctgtgaccaa gacataatgc ttttacaagg ggagaaacac 87300gtttagagac attaaccagt tttgccaaat tcacccatgt agtaggtaaa acttctcacc 87360tgaaccaaaa tcttctaact caaaattcca gggtgctatt ttacctatga aagaggttgc 87420ctgtttaaaa taatcagcag ctgaacaaat tctttattat tcacatcaag ttggtgtacc 87480tagagcttat ttaggatgat gaccatcata cgccttttcg attatcttcc agctcattaa 87540tattttgttt agctttatct atacttgctg ttaaacccat atattgagta ttttatttca 87600ttgattgtta tttttcagtt ttatttgact cttttttttt ttcaatttga ctcattttta 87660tagattccag ttccctggtg tacttcttgt catctgtttt tgagtatctt aatcacagtt 87720actttaaagt ctgtcgaata actccaatat ctaagtcacc tatgagttta tttctattgt 87780ctgtttcttg gtatgcatgg gtaaatttta actgaatgtt ggacagtcta tgggaaaaat 87840tataggtacc ctgtaccttc ctctagagag gattccagaa tgtatttgga caggcagcag 87900aggggaagat cacctcagtg cagccagcga ggacaaagta gattgaaggc tagtttgcag 87960ttttttctgt aagactccag cttctagttt cacccgtatt ccaggaaagt cactttccag 88020gagtgccctg atgtttcttc ttttgcgctt ggatctgctt ttgccactga atgcttcaaa 88080aattagcctc ttgccctaaa cagcttaatc agtcaattct tcgactgaat agtcagcact 88140aagcgtcagg cttctctctc ccttttttct gggatcttgg gcccacaaat ctgactgtct 88200tggcagccac actcttcttt tgtctccaca gttctgtgag attgcctgat gctccgctgc 88260tatccttaca tgcggtcttc tctttggctt cctcaggaaa cagcaatgcc tggaattctt 88320ggctgccttg acagctctct gatactttat aacagaggca tgtttgtgtg gggtgtgtgt 88380gtgtgtttaa aaatctattt ttctcattgt taatagaagc tgaggtttgc tataagctac 88440ttacagctgg aggcagaagt ctgtatgtat ttgttaaaca gtcatgttgg ttaagatgct 88500tgattgaggg attctgcctc ttaagagatc taacagtgag gattgcatga tctttcaaaa 88560aaatccttaa aaaagacaat ggtttttatt cgtcatttat tttgttttta aaaaagcccc 88620aaagaaagag tatcctctgc cccaagttaa ctgcctatga ataatagttc acattgctta 88680ggattttgaa ctttccagaa tgtttttacc tataatattc accccacaaa tatatgttgt 88740atgacttttg tatcattttt aaaagacatt tatatttttt tgagataggg tcttgctctg 88800ctacccaggc gggagtttgg tggtgtgacc acggctcact gcagcctcca tttcctgggc 88860tgagtggtcc tcctgccttg gtctcccaaa ggctaaggca ccctgccgat atatacttgt 88920tctatagatg atgaaaccaa gaaacatact aagtaaatgg cttaaaatca caatctaatt 88980ataggcagga ataactaaaa tcaggcttcc caactcctag ccctttccaa tacattaaag 89040tgacctcgat tcatctgtgt atacattatg gacctgtagt cacactccta agtttaactt 89100tccaatattg ctttatctta actctgtaac tttctatcag ggatctttcc agtggcatgg 89160gaaatgtgta tgtcctctga gaggcacaca tacatgcact tgggcaggtg cacagatgga 89220tctatgttcg gagcctgccc attcagtctg aataaaaagg aatcatggta gtttgatttc 89280tgggtgcaga gcaatgctac agacattcta gtatggttgt tggagtctaa agacagtgtt 89340cccatgcaga taggtgacat gaaaaatatg tagcgttcat ttcctagaat gtcatagcca 89400cggagtctta gaataactct tggcatccaa gttaaaagct ggatcacttc ctccaaatag 89460tcttcactca cttccgggag tgggttgtat tcacctttag ctctgttcac gagacactcg 89520tgggatattc ctagccttct gcccccttag ttacgggcat tgccgtctca ctgctgtata 89580accagaggcg tgggagctgc agctgtcaga atcctaatcc cttctgcctg gactcgttac 89640cagtggtagc gagtgtgtat agtagtaaac tgtgctgcga tgctagcctt tcagtcacta 89700ccagaggtcc cagcagcaaa aacaaaaatt gtggatgata cattttaacc ttctagaaac 89760tcagctaact cctttaggta tgtccaggct ttccatttaa atatcagcct taaggcagac 89820atgtctttct ttaatggaag agatctaaat tgggagcaaa acatgtgacc acttttgcta 89880tcgttttagc tgtgtagcct caatgacata atttaacctt ggttttcctg atttgcaaac 89940tgtagggaaa ataatgtctt ctaacttgct gaagtatgct gtaaaagaaa atgtggtggc 90000tgtaaaatgt gctttgggat tctcggaaga aaggagtgct atattaattc atggtactgc 90060aattgttatt ttaccccaga ttgagaagta aaatatcaaa tcgttatttt ctaaagtttg 90120aataactgtt aaatttattc agataatctg ctctattcaa gaggcataaa tagtttcttc 90180agttttaaat atatcggctg tgatggtatg gactccttaa ggtagacgct gctcaaaaga 90240ttatctggca atgaggctgt ttgacattgc ataggtgcta atgatgcatt gatggtaaca 90300agtgaacttt caatgtttta atcgcctctt aaattttagg attgcagtct tactagtcaa 90360tatgaagatg atgcctttct ttgttacaac tgaggtagtc tctggtgaag aatatgtttt 90420ctttctgtag tagcttttta aaatttctga ctcagaagct tttgttctga ttttaatttg 90480ttctagatag tgtttagatg gtctttctta agtcacgctt cgttaatata tgatatttta 90540atataatttt gtctgctgta tttgaagtta agctttatta agaaactata aaagcactcc 90600atacatattt tgaattatta ccatttgata ttttaatgta ttatctatct agttttcaag 90660aaataatgaa gaaaacatca atatatcacc taccatcatt tcattacttg atggattcag 90720aagctcaaga aggtaagtca ttttatgttt atgtagctta ccaattctaa gaagacaaat 90780aactaataga atataaagtg tcacctttgt agtattcatt aatgtcttaa atggagctga 90840cacttttatc aatggcatat tatgtcttgg tttatatttg tgagcctgca aacatcttga 90900atcatgttta agtctattac tataccatga tcttgatgat atgttaaaga tacttttgac 90960agattaaatt aaatatatag acttactaag catgtacagg gtttttgatc ttcaaataag 91020gaatacattt ttttctcaag tgctgtacac taaatgtatg atgtgatttc taagcaaatt 91080ttatttcaga taagaagctt gcaagaaaga taaaaatagt tttatgtgaa aactataagt 91140ttttactggt ggttggatga aaggtgaatt taggctatac agacatctgt agcctgagtt 91200tatggttcct ctaactattt attagaggca aatacataat gtaatttgac ctctggatgt 91260aaagagatta agtttgaatt ctactatcag tgtggttatt tgaaattgaa ccatcatttt 91320tatatagtct taagtatcaa gcgtgacttt gcatgggtta tgtaatataa ctgtccccat 91380tgtcttgaga tcaaaagtac agacatacac tcttgacaga ctagaaaaat taccatattt 91440ttgtagagca gttgtaatac aaatctaaat atcttgattt cttttgttct ctccttatct 91500gcacacactg tagatgtttt ctgaacacta tagttctggt ttttctgttt ttggccattc 91560caggtggctt ctacctagga gaacacatct ttaggccaag aaagctgggt gtatgcccag 91620tgttggcagc ctacctggct taaacacaaa tagttttacc aagcgattct tgcaaaccta 91680accatttaag tcgatcactt agagaaccca gccatttaga tgatttgaat gaagacgcaa 91740tttctgtatg gtttatgaat aaaattatat cattatcatt accattagta acaattcttg 91800gtttcaactg tgtagaagat aatttggcaa attacaaagg aagtggaaga agttcaaggt 91860aaggttgaga cggaactagc attacaatgt tttacaacac aaacaaacac actctgtaat 91920catctatata gccaaatgaa gctttaaaaa tgtgttcaca cacacagagc taagggaatt 91980aaggatgtgc ccttggagca gatcaggtgg catttagttg gaaagccatc accaagtaga 92040ggaacactca gttctcaagt actgaaggat actgactttt cctgccctct tttatactta 92100gaatcctgtg cttggaaagg atggagcaaa cccatagctc agtgctcata tttcaacact 92160ggcatgaaga tccaagaact ataggatgtc acttatttat acacagatta aaattagggc 92220tatggtgggt gctaaaaaat tcttctgtat gtgtttctaa ggctatattg taatctaagg 92280tgtctaaaac cttacccaca ttaactattc taaatatcct atgttgcctg cctcatgcca 92340gcagcctctc tccagctgtt agtacacgta gcaatctgtt atagaatagt agggtcaccg 92400gaggacctgc agccttgggg cagattcatc cttgcccaag gctcagtgat tctggctctc 92460aatgaccctc gagtctgtca cgtcagctgc ttacttgaca tcctacttac tacctcttgt 92520cagtttaggg ccttcaagac atagatgcca aaatggggct aagcatgtaa gggatttact 92580gggggaaatg tttgtggagg ataaggaaga aaacagaagt aggctagggg agccttcaga 92640gcccaacatg ggtccagtgc ccgtgagagg agacagaaga cagattggat gggaagagtc 92700tccaattgca acacaattct gagacagtct caggtgggct gatgaaaatc cctgagcagg 92760agttgcccac tagaggagtg tcctatatgg aacaggaact ggctggcttg agtaggcagc 92820ctcaggagga acatggtctc ccaggagcca caggtgcagc aatgggcagt gttgggcaac 92880tctgctcctt gtggcaggtt ctcttaatct gagtggcaca ccagggtaat atgtatttaa 92940aataattttc ctttacaggt ttgcccttcc acctatattt tcgatctcac tcaatggcaa 93000catcactccc caggctaccc agaaacctgg aatcatcctg attttctccc tttccttcac 93060acccttcatc attacatctc attgatttta cattcctgat atgtttccct acgccttgtt 93120ccatttccac catcacttac cttgattctt cattatctct gctcctgact gctgttaacc 93180accttctaat tggctgtctc gctctgggcc aactcccttt ctttaccctc tctagttctt 93240ttcccacact gtaacctgca ctgcatgatc ttttaaaaat ccaaatcttg tcacatttgt 93300cttctgcttg aaattcttca gtgcctcatt atttcttaca ggataaaatc aaggcttctt 93360agtatggtgt aatgtgtttt accttgtgct actatcctca tttatcctat attctggttc 93420tcagggtttc caaaattggt caggagactt tttgccagtg ggcttttgca catgtcattt 93480cctgtatatg gcaggtcttc ccttctccta tttgacaaac tcataattag gaaggctcag 93540ataattcttt cctgagctct ctgagaggtg gaactttcca tttttgcatt gcctactgaa 93600gtctctgtta tagcactttt actgaacctc tcttatatag cacttggtct gccttctaat 93660tcatagtgcc caggtactta ataagtgagt gaatgaatga atgaatgaat tagctataac 93720ataaccgtat tggctgactt aaatttgctc atgagtgatg atcttgtatt tctgatcata 93780agtcagttga ctcccttcag gacctaatat aatgttctcc tcaaggcatc acattaaaaa 93840ttttcaggag catcttcata aatctttgca gaattggatt aatgggtaat atggttttta 93900taaacagtat tgcattaata tatatggaaa tttatgtaat tctaccactg ttttaagaaa 93960cctatcaaac tacagtctga ggcaatatat ttaatatact agttggagta ctatagcaca 94020tataattact aaagtgagaa taatttatta acacatttta aacattttac aagtaacatg 94080aataattatc ttccagataa gactaataaa ttttctacat gttgaataaa aataactctt 94140gaatatacca gtctacttca gatgtcttca aaactcagat ttgaacgtca agtttgaatg 94200tcaattgtcc acccctaggg gaggcatacc accactttga aagcacacac gttcatactt 94260ggctccttga cccaaggcct tcatttagac tcttgtcatt tttcatctag actgttccaa 94320taatcttcta attagcttgt aaggtcacca gttcttcagc tcctgctgtc agatgtagtt 94380cttaaatatc aaatctgatc atgtcaattc ctgttctaat aatctttatt agctcctcct 94440caccttatag aacaaaggtc aatgttgcac ataaatttaa tcccaaccta cttcttcagg 94500cttattttct atagttcccc tccatgcacc ttgtgtaata ttcattataa tccctaaaga 94560cacaatccaa aatgccatga tcctgaatgt tgaaatcccc aaagaacaaa tgcccttaca 94620gctaattgaa ttcccaaacc ataatgatag atttggaatt aggtgccatc aaaacttcta 94680aaggtggatt tcaaggtgtt agcaacagtt tctttttcca ttcagcccaa tacatttgcc 94740ggaaaattca gatgaatgga ttgcctgtag aatatagtaa catggaaaac ttcagtttat 94800aacgcattat ttgtctgcat tggcattcct tctagctgat gaaattctag gaggtcttaa 94860tgagttaaag ctgaatttgc ctgatgaagc cagcaaagtt actgactcta aaataactat 94920gtgcatggta ggataagaaa gcacacaatg atgttgctgt tcgatcacca gtattgtttc 94980agccaaatct gtggtctgta tatgagtgca tgagcaaagg atttccatgt acccaaaaca 95040acacagaagc atgacacaga gataggaaat ttaatagaaa atgctcatgt cagtgtgtat 95100tgaataaaac tagaatttca aaaagagcag catcatgtag aaaatcaatg tgaatgtatt 95160cttcaagaag agccatgtcc taaaagaaaa gaaacagtta ctcatctcca cgcaagactt 95220cagaatacag ttaatgattg tggaagtcgg ccagctctta tgggctacct ccgtgcagtt 95280gcccataatt tatccctgta atgcgctttt tcatatgttg aattttcttt ttagtttctt 95340tgggaaggtt ttgtttttct ttttcctttt ttaaagtttt tttcctcacc attttaaatc 95400atcagcatta ttttttacaa ctcactatgg tatgctacgt gtttcatctt tacatttcca 95460aaactagagg cataaattgt atgagctttt agagagttct aatttgtttt atgcactttt 95520tgttttttgc aaaattgact ccatggaagt gcattttaat aacactgacc ttgtgtgtaa 95580gcattgtgca tatatatgta aaaaccttaa aacttcctca ataaataaag ggatatcctt 95640tttctgcatc tgcatttgtg aaagataaca tttttcaata tctcagctct ttaggcaact 95700gtatatgact gtatatgtgg tggtgaccca ttgttggtat ttaattttaa tttttttagc 95760aaggggcgtc tcactatgtt gcccaggctg ggctctaacg atccttccac ctcagcttcc 95820tgagtagcgg ggactacagg cgtgagccac tgcattcagc tccactgtag gtttggatct 95880gtttcatcag aagacttagg ttgttcatca ggatatttca agtgaccaaa ctatataagt 95940gatttcttta tgaatacaat ttctctgctc ataactgtta tgcctgtgta actgtgggta 96000gcacacctga gtgcttatca ttgcaaaatt atgtatatac aattttattg tataaagtag 96060cccacaaagt gttctgttgt gtttttatgt ttctaaactc ttttaaaaat gtaaatatgt 96120tttaaagaac ttttagaatt atttttatca gaattatgta tttggggttt tgatctttca 96180ggattcaaca ctggggatta tggcatcaga aactatcttt tgggattctg gcccaaacct 96240ctagccttgt accctcgctc catagatctt ctaacagctc ctgggcttaa atatctcctc 96300ttttatgatg cttctcctga ttcaaccaac cagtggtgct tcccatagca ttttagaaag 96360acctgtttta tagcatttat caaggtattg acatttgttc agttgtctgc cccttccgtg 96420atctcagtag caaacttgta gtacaactaa tttaggttct ttatttttcc acttgcatcc 96480tgagcaacct caataaaagg tgcattttct ttcttttgta aacacaaagt gtagcattgt 96540ttggtctact tgattagagt tacaacctct gtacattctt gaaactaaga agaccagcag 96600acagagaata tctgtagaat cttctcagga attggtttca acataagggt ccaagacctt 96660ggcattgctg aggttttcag aattctatct tttaagttaa gcctgtgact atttactaat 96720ctgaaaatat ctttaacttc ttttttttaa tctgtaggca tttctaagaa gcacatgaca 96780ttcagctttg aatagaagat tcatttgaat gacagtttct ttcatttagt agaggggatt 96840ttactttaaa ttttgctgac aggttttcgt gacagccctc aaatggaagc ttgaattc 9689842129DNAXenopus sp.misc_feature(74)..(74)n = a, c, g, or t. 4gaagaagagg agaatggtag gacggagcca gcagctttat gacaaaatag gttgaaaatt 60gttgattgag gcanccgctg tgggatgtgc aaagattgta agctgttgtg agtggcagag 120atctctggcc ccatttggat ccccgtacct atgtttgtta cgtaattgtt ttactggcga 180acaaggtctc agttaaagga tgaagctcat tcggtgagac ttttattgac caaaacatag 240tcaatatttt tgttgtgatt tgcaccgaac tgcattgttc atcttcattc aatgcctaac 300atagaagcgg acagagttac aagcgttccc gaaaataacg actgcaaatc caagagtcag 360cctctgagga ataatctcca tgaaactgta aaatcctaca gtatacaagg tgctgccaca 420gcaaatatcg agcctcctgc agaaagacct tatccctggg gatgtcctgt gacacacaca 480aaggaaaagt tctataccat ctgcgcagac tacgcctttt tgaatcaagc aacatctctt 540tgcaaatcgt ctagttctgt ctgcagctca agctcagagg acaaatctgc tctgagcaat 600acaataaatt acattgatct tcagaccagc gaatcagatt ctgtatacaa cgaggatgca 660agcttggagt ctttatctag caatcttggt acacgtccgc ttgcctggga aattgataaa 720tcagacttca gcacaatgac ttccaagtta aaaagatcag gtgtaaaaaa acaaacacct 780aagaagaaac ctgacaggaa ggcaaaacca ttaagggact gtcctcaaca cttaatcctg 840gatgatgtta aacagcggaa agttctagac ctcagacgat ggtattgtat cagtcgaccc 900cagtacaaga cctcgtgtgg catttcttct ctggtatcat gctggaactt tctttacagt 960actctgggag caggaagcct cccaccaatt actcaagagg aagctttaca tattttgggc 1020tttcaaccac cctttgaaga gattaggttt ggtcctttta ctgggaacac aactttgatg 1080agatggttca gacaaatcaa tgatcatttc catgtaaaag gatgctccta tgttctgtat 1140aagccgcatg gcaagaacaa gacagcagga gaaactgctg ttggggcact atcaaagtta 1200acacaagggt taaaagaaga ctcaacagcc tacgtctatc attgtcagaa tcattatttt 1260tgcccaattg gttttgaggc aacccctgtg aaggcatcca aagcatacag gggccaactt

1320ttcccgcatg aagtggagta ctggatttta attggtgagc caagcagaaa acaccctaca 1380attcactgca aaaagtgggc agatattgtt actgacttaa atactcaaaa tccagaatat 1440tttgatatta gacacactga aagaggcctt cagtacagga aaacaaaaaa ggttggagga 1500aaccttcact gccttctggc atttcagaga ctcagctggc aaagatttgg tccatggccc 1560ttacagcttg gaacccttag gccagaaccc cagccacccg tacaaggaag aagaatccct 1620aaatctgaaa gtgaggataa tgtctccaag aaacagcatg ggcgtctggg gaggtcattt 1680agtgctggat ttcagcaaga gcttgcatgg aaaagaatgt gtaatatacg tgaacgcagg 1740ggcagtggct cacctgaaag tgatacggac tgagaaggaa atgattaaat tatacaaagt 1800cagtgttact tgtagttttg ggttcatggc actacgatta aactaaacat tagtcatata 1860atgctggaca tggttggcag acattatttg tagttttggg ttcatggcac taccattaag 1920ctaaacatta gtcatataat gctggacatg gttggtagac atctatagtg ctctcccatt 1980aatcataaaa cctttgcaaa ctttttacaa tcatttatga acttattgct caaatgccat 2040tcctgatcta cagtatactg ggtattgtat actgccatgt caggagtatt ttcattaatt 2100aataaaattg gagtttaaaa tcaaaaaaa 21295493PRTXenopus sp. 5Met Pro Asn Ile Glu Ala Asp Arg Val Thr Ser Val Pro Glu Asn Asn1 5 10 15Asp Cys Lys Ser Lys Ser Gln Pro Leu Arg Asn Asn Leu His Glu Thr 20 25 30Val Lys Ser Tyr Ser Ile Gln Gly Ala Ala Thr Ala Asn Ile Glu Pro 35 40 45Pro Ala Glu Arg Pro Tyr Pro Trp Gly Cys Pro Val Thr His Thr Lys 50 55 60Glu Lys Phe Tyr Thr Ile Cys Ala Asp Tyr Ala Phe Leu Asn Gln Ala65 70 75 80Thr Ser Leu Cys Lys Ser Ser Ser Ser Val Cys Ser Ser Ser Ser Glu 85 90 95Asp Lys Ser Ala Leu Ser Asn Thr Ile Asn Tyr Ile Asp Leu Gln Thr 100 105 110Ser Glu Ser Asp Ser Val Tyr Asn Glu Asp Ala Ser Leu Glu Ser Leu 115 120 125Ser Ser Asn Leu Gly Thr Arg Pro Leu Ala Trp Glu Ile Asp Lys Ser 130 135 140Asp Phe Ser Thr Met Thr Ser Lys Leu Lys Arg Ser Gly Val Lys Lys145 150 155 160Gln Thr Pro Lys Lys Lys Pro Asp Arg Lys Ala Lys Pro Leu Arg Asp 165 170 175Cys Pro Gln His Leu Ile Leu Asp Asp Val Lys Gln Arg Lys Val Leu 180 185 190Asp Leu Arg Arg Trp Tyr Cys Ile Ser Arg Pro Gln Tyr Lys Thr Ser 195 200 205Cys Gly Ile Ser Ser Leu Val Ser Cys Trp Asn Phe Leu Tyr Ser Thr 210 215 220Leu Gly Ala Gly Ser Leu Pro Pro Ile Thr Gln Glu Glu Ala Leu His225 230 235 240Ile Leu Gly Phe Gln Pro Pro Phe Glu Glu Ile Arg Phe Gly Pro Phe 245 250 255Thr Gly Asn Thr Thr Leu Met Arg Trp Phe Arg Gln Ile Asn Asp His 260 265 270Phe His Val Lys Gly Cys Ser Tyr Val Leu Tyr Lys Pro His Gly Lys 275 280 285Asn Lys Thr Ala Gly Glu Thr Ala Val Gly Ala Leu Ser Lys Leu Thr 290 295 300Gln Gly Leu Lys Glu Asp Ser Thr Ala Tyr Val Tyr His Cys Gln Asn305 310 315 320His Tyr Phe Cys Pro Ile Gly Phe Glu Ala Thr Pro Val Lys Ala Ser 325 330 335Lys Ala Tyr Arg Gly Gln Leu Phe Pro His Glu Val Glu Tyr Trp Ile 340 345 350Leu Ile Gly Glu Pro Ser Arg Lys His Pro Thr Ile His Cys Lys Lys 355 360 365Trp Ala Asp Ile Val Thr Asp Leu Asn Thr Gln Asn Pro Glu Tyr Phe 370 375 380Asp Ile Arg His Thr Glu Arg Gly Leu Gln Tyr Arg Lys Thr Lys Lys385 390 395 400Val Gly Gly Asn Leu His Cys Leu Leu Ala Phe Gln Arg Leu Ser Trp 405 410 415Gln Arg Phe Gly Pro Trp Pro Leu Gln Leu Gly Thr Leu Arg Pro Glu 420 425 430Pro Gln Pro Pro Val Gln Gly Arg Arg Ile Pro Lys Ser Glu Ser Glu 435 440 445Asp Asn Val Ser Lys Lys Gln His Gly Arg Leu Gly Arg Ser Phe Ser 450 455 460Ala Gly Phe Gln Gln Glu Leu Ala Trp Lys Arg Met Cys Asn Ile Arg465 470 475 480Glu Arg Arg Gly Ser Gly Ser Pro Glu Ser Asp Thr Asp 485 49062841DNAGallus sp.misc_feature(235)..(237)Translation initiation codon (ATG) 6ggacactgac atggactgaa ggagtagaaa gcaggtgagc gctcgtcgtg gcttctcccc 60cccctgcgtc gcgcactgcg tctgtttccg gcgcgggcac attccccgct ccgccgcggg 120cccgcgcagg tacctcacct tgcagtaaca atggaggcag cgatgcaaac tatgtggtaa 180ttaaaaataa gcaaaaccat tcctcgcagt acaagacgct tccaatatta ttcaatgcct 240cacatctcag aagatgaaaa ggagaatggt tctggaaaca atggaaacac tgaaaagaaa 300cctgggaaag aatcctcaga agcttctctt cgtgatccta taaagtcgta ctgcatctca 360gatgcctcca ctgtgtcttt ggtgtccagg ggagatggac attacccatg gggatgtcct 420gtgactcaca cacgagagaa attttatacc atttgctcag actatgcttt tttaaacaga 480gtaacatcta tttgtaaaag cccaagtgct tcagttaacg cctgcctgtc aggcagtgct 540gccttaaacg ttggaaataa cacacctagc ttactgggca ttcaaactgg tgcttcggag 600ataatctaca gtgaagatgc taacttggaa accttgtctg gcagccttgg aaagcttcca 660ctggcatggg aaattgacaa atcagaattc aacagcgtga ctgcgaatca taaaaacaaa 720gcaggcaaca tgaagaaaca agtggcaaag aaaaagtcct cagacaaaaa aagcaaacag 780tacaaggagt gtcctcagct gtctgctctt gaagatgtga aggagaggaa agtgttggac 840ctccgaagat ggtactgtat tagccgacct cagtacaaga cttcttgtgg aatttcttca 900ttagtgtctt gctggaattt cttatatagt acgctgggag ctggcagttt accacctatt 960actcaagaag aagctttgca tatattgggt tttcaacccc catttgagga gatcaggttt 1020ggtcccttca ctggaaatac gactttaatg agatggttta ggcaaataaa tgatcacttc 1080catatcaagg gttgctcata tgttctgtat aaacctcatg gaaagaacaa gacagctgga 1140gaaactgctg tgggggccct tgcaaagcta acacgtggac tgaaagatga atcaatggcc 1200tacatctacc attgccaaaa ccattatttt tgcccaattg gatttgaagc aactccagta 1260aaagctagta aagcgtatag aggtcgtgtt ttgcagcaag aagtagaata ctggatctta 1320attggagagc cgagcagaaa acatccaacg atacactgta aaaggtggac agatattgtc 1380actgacctaa acacccaaaa tccagagtac ctagatattc ggcacctaga gagaggactg 1440cagcatcgga aaacaaagaa ggttggagga aatcttcatt gcatcatcgc ctttcagaga 1500cttaactggc aaagatttgg tccttggaat attccatttg gaagtgtcag acaggataaa 1560caatcccaaa cacaaggaca aggtattgcc aaatctgaga gtgaagacaa tatctctaaa 1620aaacaacatg gacgactggg tcgatctttc agtgctggtt tccatcaaga atctacatgg 1680aaaaagtcta gtcttcgtga gaggaggaac agcgggtatc agagctataa tgattatgat 1740ggagatgatt agaattaact ttaggtaata gagtttatat atcaaagtta gttttaatca 1800acacagaata ggggtttatt agtcctagga tacatgtgaa tagaaaatat ggcataagat 1860acagctttgt aatccttaaa tcaattatga attatatggt tgcagtggat aaaagagcag 1920attgaaatta gccaatgtaa taaacagatt tcattgaaaa tacttgatat tcagaagcat 1980gaaaatgtat tatatgactt tataaaaagg gttatactgc atatggtgta aggataaaag 2040taaacatttg ccttcctttt tagcactcca ttttgttaag gctgctgata tccagtgaga 2100agaaagaaat tgaataggtt agaaaacctt gtcagattaa caaaattgaa tgtatattct 2160caatctagtt gtcagtagaa ttctgtgagt cagataatcc tgttttgtag gtagatccca 2220gttatttttc ccatagctag atacctgttt taaactgaga agaattgctg gtggcaagga 2280aggtttgaag atggacattt actgcttttg ctctgtggat atggtagcag attttctatc 2340ctgtgagctc tggtgagcag tgactgcata acacaggctt gtgaaaatca tttttataaa 2400gctgcattta acctgagccc aatgaactgg ctgaacagtg tgttctgctg gcaattcttt 2460tccttgttca gtctcaaaac tcctgttgtt tttgtgctgc tctcttgatt ttgtatgaag 2520gtgatgcaag tgccgacaac tgctggcagc ccttatgata tacctctatg ccagcaaaca 2580atccaagtct tttcaggtgt ccatgtgcag tttttttttt ttcctttctg gtttattcag 2640ttgtttgccc aaatgcatct cgacagttgt aactttgtgt gcgaatgtcc acacctgctc 2700aaggattttt ttttttttac ataaaacaat ttgtcatgta atgcagggtt tttgtaggtt 2760gatgctgttg ttaaccaaaa atggagggag acttttggac tttcgttcat tcaataaaat 2820ttgttttatt taaaaaaaaa a 284173038DNAGallus sp.misc_feature(235)..(237)Translation initiation codon (ATG) 7ggacactgac atggactgaa ggagtagaaa gcaggtgagc gctcgtcgtg gcttctcccc 60cccctgcgtc gcgcactgcg tctgtttccg gcgcgggcac attccccgct ccgccgcggg 120cccgcgcagg tacctcacct tgcagtaaca atggaggcag cgatgcaaac tatgtggtaa 180ttaaaaataa gcaaaaccat tcctcgcagt acaagacgct tccaatatta ttcaatgcct 240cacatctcag aagatgaaaa ggagaatggt tctggaaaca atggaaacac tgaaaagaaa 300cctgggaaag aatcctcaga agcttctctt cgtgatccta taaagtcgta ctgcatctca 360gatgcctcca ctgtgtcttt ggtgtccagg ggagatggac attacccatg gggatgtcct 420gtgactcaca cacgagagaa attttatacc atttgctcag actatgcttt tttaaacaga 480gtaacatcta tttgtaaaag cccaagtgct tcagttaacg cctgcctgtc aggcagtgct 540gccttaaacg ttggaaataa cacacctagc ttactgggca ttcaaactgg tgcttcggag 600ataatctaca gtgaagatgc taacttggaa accttgtctg gcagccttgg aaagcttcca 660ctggcatggg aaattgacaa atcagaattc aacagcgtga ctgcgaatca taaaaacaaa 720gcaggcaaca tgaagaaaca agtggcaaag aaaaagtcct cagacaaaaa aagcaaacag 780tacaaggagt gtcctcagct gtctgctctt gaagatgtga aggagaggaa agtgttggac 840ctccgaagat ggtactgtat tagccgacct cagtacaaga cttcttgtgg aatttcttca 900ttagtgtctt gctggaattt cttatatagt acgctgggag ctggcagttt accacctatt 960actcaagaag aagctttgca tatattgggt tttcaacccc catttgagga gatcaggttt 1020ggtcccttca ctggaaatac gactttaatg agatggttta ggcaaataaa tgatcacttc 1080catatcaagg gttgctcata tgttctgtat aaacctcatg gaaagaacaa gacagctgga 1140gaaactgctg tgggggccct tgcaaagcta acacgtggac tgaaagatga atcaatggcc 1200tacatctacc attgccaaaa ccattatttt tgcccaattg gatttgaagc aactccagta 1260aaagctagta aagcgtatag gttgctggat ttggactcgg gagacctggg ttcggttccc 1320agttcaaccg cagacttcca ttgtgatttt agaggtcgtg ttttgcagca agaagtagaa 1380tactggatct taattggaga gccgagcaga aaacatccaa cgatacactg taaaaggtgg 1440acagatattg tcactgacct aaacacccaa aatccagagt acctagatat tcggcaccta 1500gagagaggac tgcagcatcg gaaaacaaag aaggttggag gaaatcttca ttgcatcatc 1560gcctttcaga gacttaactg gcaaagattt ggtccttgga atattccatt tggaagtgtc 1620agacaggata aacaatccca aacacaagga caaggtattg ccaaatctga gagtgaagac 1680aatatctcta aaaaacaaca tggacgactg ggtcgatctt tcagtgctgg tttccatcaa 1740gaatctacat ggaaaaagtc tagtcttcgt gagaggagga acagcgggta tcagagctat 1800aatgattatg atggagatga ttagaattaa ctttaggtaa tagagtttat atatcaaagt 1860tagttttaat caacacagaa taggggttta ttagtcctag gatacatgtg aatagaaaat 1920atggcataag atacagcttt gtaatcctta aatcaattat gaattatatg gttgcagtgg 1980atgacatctg atacatgaac tgacagataa gcacagatta ttgtactttt gtaatcaaaa 2040gcagatatga cagctaaatc aatcacttat tttgaagtta ctatactata tcctgatctg 2100tgagaataaa agagcagatt gaaattagcc aatgtaataa acagatttca ttgaaaatac 2160ttgatattca gaagcatgaa aatgtattat atgactttat aaaaagggtt atactgcata 2220tggtgtaagg ataaaagtaa acatttgcct tcctttttag cactccattt tgttaaggct 2280gctgatatcc agtgagaaga aagaaattga ataggttaga aaaccttgtc agattaacaa 2340aattgaatgt atattctcaa tctagttgtc agtagaattc tgtgagtcag ataatcctgt 2400tttgtaggta gatcccagtt atttttccca tagctagata cctgttttaa actgagaaga 2460attgctggtg gcaaggaagg tttgaagatg gacatttact gcttttgctc tgtggatatg 2520gtagcagatt ttctatcctg tgagctctgg tgagcagtga ctgcataaca caggcttgtg 2580aaaatcattt ttataaagct gcatttaacc tgagcccaat gaactggctg aacagtgtgt 2640tctgctggca attcttttcc ttgttcagtc tcaaaactcc tgttgttttt gtgctgctct 2700cttgattttg tatgaaggtg atgcaagtgc cgacaactgc tggcagccct tatgatatac 2760ctctatgcca gcaaacaatc caagtctttt caggtgtcca tgtgcagttt tttttttttc 2820ctttctggtt tattcagttg tttgcccaaa tgcatctcga cagttgtaac tttgtgtgcg 2880aatgtccaca cctgctcaag gatttttttt tttttacata aaacaatttg tcatgtaatg 2940cagggttttt gtaggttgat gctgttgtta accaaaaatg gagggagact tttggacttt 3000cgttcattca ataaaatttg ttttatttaa aaaaaaaa 30388505PRTGallus sp. 8Met Pro His Ile Ser Glu Asp Glu Lys Glu Asn Gly Ser Gly Asn Asn1 5 10 15Gly Asn Thr Glu Lys Lys Pro Gly Lys Glu Ser Ser Glu Ala Ser Leu 20 25 30Arg Asp Pro Ile Lys Ser Tyr Cys Ile Ser Asp Ala Ser Thr Val Ser 35 40 45Leu Val Ser Arg Gly Asp Gly His Tyr Pro Trp Gly Cys Pro Val Thr 50 55 60His Thr Arg Glu Lys Phe Tyr Thr Ile Cys Ser Asp Tyr Ala Phe Leu65 70 75 80Asn Arg Val Thr Ser Ile Cys Lys Ser Pro Ser Ala Ser Val Asn Ala 85 90 95Cys Leu Ser Gly Ser Ala Ala Leu Asn Val Gly Asn Asn Thr Pro Ser 100 105 110Leu Leu Gly Ile Gln Thr Gly Ala Ser Glu Ile Ile Tyr Ser Glu Asp 115 120 125Ala Asn Leu Glu Thr Leu Ser Gly Ser Leu Gly Lys Leu Pro Leu Ala 130 135 140Trp Glu Ile Asp Lys Ser Glu Phe Asn Ser Val Thr Ala Asn His Lys145 150 155 160Asn Lys Ala Gly Asn Met Lys Lys Gln Val Ala Lys Lys Lys Ser Ser 165 170 175Asp Lys Lys Ser Lys Gln Tyr Lys Glu Cys Pro Gln Leu Ser Ala Leu 180 185 190Glu Asp Val Lys Glu Arg Lys Val Leu Asp Leu Arg Arg Trp Tyr Cys 195 200 205Ile Ser Arg Pro Gln Tyr Lys Thr Ser Cys Gly Ile Ser Ser Leu Val 210 215 220Ser Cys Trp Asn Phe Leu Tyr Ser Thr Leu Gly Ala Gly Ser Leu Pro225 230 235 240Pro Ile Thr Gln Glu Glu Ala Leu His Ile Leu Gly Phe Gln Pro Pro 245 250 255Phe Glu Glu Ile Arg Phe Gly Pro Phe Thr Gly Asn Thr Thr Leu Met 260 265 270Arg Trp Phe Arg Gln Ile Asn Asp His Phe His Ile Lys Gly Cys Ser 275 280 285Tyr Val Leu Tyr Lys Pro His Gly Lys Asn Lys Thr Ala Gly Glu Thr 290 295 300Ala Val Gly Ala Leu Ala Lys Leu Thr Arg Gly Leu Lys Asp Glu Ser305 310 315 320Met Ala Tyr Ile Tyr His Cys Gln Asn His Tyr Phe Cys Pro Ile Gly 325 330 335Phe Glu Ala Thr Pro Val Lys Ala Ser Lys Ala Tyr Arg Gly Arg Val 340 345 350Leu Gln Gln Glu Val Glu Tyr Trp Ile Leu Ile Gly Glu Pro Ser Arg 355 360 365Lys His Pro Thr Ile His Cys Lys Arg Trp Thr Asp Ile Val Thr Asp 370 375 380Leu Asn Thr Gln Asn Pro Glu Tyr Leu Asp Ile Arg His Leu Glu Arg385 390 395 400Gly Leu Gln His Arg Lys Thr Lys Lys Val Gly Gly Asn Leu His Cys 405 410 415Ile Ile Ala Phe Gln Arg Leu Asn Trp Gln Arg Phe Gly Pro Trp Asn 420 425 430Ile Pro Phe Gly Ser Val Arg Gln Asp Lys Gln Ser Gln Thr Gln Gly 435 440 445Gln Gly Ile Ala Lys Ser Glu Ser Glu Asp Asn Ile Ser Lys Lys Gln 450 455 460His Gly Arg Leu Gly Arg Ser Phe Ser Ala Gly Phe His Gln Glu Ser465 470 475 480Thr Trp Lys Lys Ser Ser Leu Arg Glu Arg Arg Asn Ser Gly Tyr Gln 485 490 495Ser Tyr Asn Asp Tyr Asp Gly Asp Asp 500 5059529PRTGallus sp. 9Met Pro His Ile Ser Glu Asp Glu Lys Glu Asn Gly Ser Gly Asn Asn1 5 10 15Gly Asn Thr Glu Lys Lys Pro Gly Lys Glu Ser Ser Glu Ala Ser Leu 20 25 30Arg Asp Pro Ile Lys Ser Tyr Cys Ile Ser Asp Ala Ser Thr Val Ser 35 40 45Leu Val Ser Arg Gly Asp Gly His Tyr Pro Trp Gly Cys Pro Val Thr 50 55 60His Thr Arg Glu Lys Phe Tyr Thr Ile Cys Ser Asp Tyr Ala Phe Leu65 70 75 80Asn Arg Val Thr Ser Ile Cys Lys Ser Pro Ser Ala Ser Val Asn Ala 85 90 95Cys Leu Ser Gly Ser Ala Ala Leu Asn Val Gly Asn Asn Thr Pro Ser 100 105 110Leu Leu Gly Ile Gln Thr Gly Ala Ser Glu Ile Ile Tyr Ser Glu Asp 115 120 125Ala Asn Leu Glu Thr Leu Ser Gly Ser Leu Gly Lys Leu Pro Leu Ala 130 135 140Trp Glu Ile Asp Lys Ser Glu Phe Asn Ser Val Thr Ala Asn His Lys145 150 155 160Asn Lys Ala Gly Asn Met Lys Lys Gln Val Ala Lys Lys Lys Ser Ser 165 170 175Asp Lys Lys Ser Lys Gln Tyr Lys Glu Cys Pro Gln Leu Ser Ala Leu 180 185 190Glu Asp Val Lys Glu Arg Lys Val Leu Asp Leu Arg Arg Trp Tyr Cys 195 200 205Ile Ser Arg Pro Gln Tyr Lys Thr Ser Cys Gly Ile Ser Ser Leu Val 210 215 220Ser Cys Trp Asn Phe Leu Tyr Ser Thr Leu Gly Ala Gly Ser Leu Pro225 230 235 240Pro Ile Thr Gln Glu Glu Ala Leu His Ile Leu Gly Phe Gln Pro Pro 245 250 255Phe Glu Glu Ile Arg Phe Gly Pro Phe Thr Gly Asn Thr Thr Leu Met 260 265 270Arg Trp Phe Arg Gln Ile Asn Asp His Phe His Ile Lys Gly Cys Ser 275 280 285Tyr Val Leu Tyr Lys Pro His Gly Lys Asn Lys Thr Ala Gly Glu Thr 290 295 300Ala Val Gly Ala Leu Ala Lys Leu Thr Arg Gly Leu Lys Asp Glu Ser305 310 315 320Met Ala Tyr Ile Tyr His Cys Gln Asn His Tyr Phe

Cys Pro Ile Gly 325 330 335Phe Glu Ala Thr Pro Val Lys Ala Ser Lys Ala Tyr Arg Leu Leu Asp 340 345 350Leu Asp Ser Gly Asp Leu Gly Ser Val Pro Ser Ser Thr Ala Asp Phe 355 360 365His Cys Asp Phe Arg Gly Arg Val Leu Gln Gln Glu Val Glu Tyr Trp 370 375 380Ile Leu Ile Gly Glu Pro Ser Arg Lys His Pro Thr Ile His Cys Lys385 390 395 400Arg Trp Thr Asp Ile Val Thr Asp Leu Asn Thr Gln Asn Pro Glu Tyr 405 410 415Leu Asp Ile Arg His Leu Glu Arg Gly Leu Gln His Arg Lys Thr Lys 420 425 430Lys Val Gly Gly Asn Leu His Cys Ile Ile Ala Phe Gln Arg Leu Asn 435 440 445Trp Gln Arg Phe Gly Pro Trp Asn Ile Pro Phe Gly Ser Val Arg Gln 450 455 460Asp Lys Gln Ser Gln Thr Gln Gly Gln Gly Ile Ala Lys Ser Glu Ser465 470 475 480Glu Asp Asn Ile Ser Lys Lys Gln His Gly Arg Leu Gly Arg Ser Phe 485 490 495Ser Ala Gly Phe His Gln Glu Ser Thr Trp Lys Lys Ser Ser Leu Arg 500 505 510Glu Arg Arg Asn Ser Gly Tyr Gln Ser Tyr Asn Asp Tyr Asp Gly Asp 515 520 525Asp 101946DNADanio reriomisc_feature(165)..(167)Translation initiation codon (ATG) 10cgttgaaaca gctaggcagt cagagaggtc gcgcagcgca ttttatataa aatattcttg 60ctgaacttgc gagtcggtgg attattttgg gactgagtca tcatatctgc ggtacacctg 120acctgttggt tattctctta accatcatcc cagttctcca ttcaatgcct aacactgtgg 180aaagtgaagg cgccaaggta tccgctagta cagatcagga ggccccatca cgggccccgg 240gacgagagga tgaacgtgag cgcagcttcc tgagccccat gatgcgagat gctctgcggg 300tacgacgggc ctccagcgca gagctccagc ttccatggac gtgccctgta acccactcca 360gggagaagtt ctacaccgtc tgctcggact atgccctgct caaccgagct cgaccagtta 420tcacatccga agatgcatca cagaccaatc ctgacagcgg gacatcatta gccaagagca 480acacagcaac atcttctcag agtcactcag ggggaataag cgtatcttta gatgggaact 540gtgatatgga ggttgtgtcc tccagcaaca agcctgtgct ggcctgggag attgacacct 600cagatttcga tgccgtttta acccggaaag ccagaacaag taatttgaag aaattcaaca 660ctaagaaaat gaaatcatct gacaggccaa gcagaaacct gcaagatgtc ccgccacaag 720cctctctaga tgaaatcaaa cagagaaaag tgctggacct ccgtagatgg tactgcatca 780gccgaccaca gtataaaaca tcatgtggaa tctcttcact tgtttcttgc tggaactttc 840tctacagtac tctcggagca ggcagtctcc cacctatttc tcaagaagaa gctctgcata 900tacttggatt tcagcctccg tttgaagata tcaaatttgg accatttact ggcaatgcca 960ctttaatgag atggttcaga caaatcaatg ataattttcg tgttcggggt tgctcatata 1020ttctgtacaa gcctcatggg aagcacaaga cagcaggaga gacagccgag ggggcgctca 1080tgaagcttac acagggtctt aaagacgaat ccatggccta catttatcac tgtcagaatc 1140actacttctg tcctgtgggc tatgaagcta ctccactgaa agcagccaaa gcatacaggg 1200gaccactgcc tcttaatgag atggagcact ggattctcat tggtgaacca agccggaaac 1260atcctgcaat ccactgtaaa aaatgggcag acatcgtgac ggacctaaat actcagaacc 1320cagaatactt agacattcgc catattgaga gaggcataca gtatcgcaaa accaagaagg 1380ttggaggcaa tctgcattgc atcatggcct tccagagagt gaactggcaa aaattgggac 1440catgggcgct gaatctggaa aacctgaggc atgatctcca tcatcaggct ccagaacaca 1500gaggccaagc ttcaacagag gacagttctg aggagcgaac ggtgaaacgc ctgggtaggt 1560ctctcagcac ggggaacaag cctgaaaatg cctggaagcg tttgtccaac acagccgagt 1620acaggcacag aggctctcca gacagtgacc tggatgaaga catcactgac taaatatgaa 1680gggccaggtg ggtttcgaca cttttattca agattattaa ccttccaggt tattagctat 1740agttaaaggt tacaatccgg tatgaggttg tgatgtaaga gttagtgctc agactggtaa 1800acttaaaaat ggaagtttga cgccaataag aatatgggaa agagctcttg tggaggacat 1860ctgtgtaata ctgacagcaa tgtgaattaa gttacactgg ctttggtgat gtgccgataa 1920ataaaggttt aaaatactaa aaaaaa 194611502PRTDanio rerio 11Met Pro Asn Thr Val Glu Ser Glu Gly Ala Lys Val Ser Ala Ser Thr1 5 10 15Asp Gln Glu Ala Pro Ser Arg Ala Pro Gly Arg Glu Asp Glu Arg Glu 20 25 30Arg Ser Phe Leu Ser Pro Met Met Arg Asp Ala Leu Arg Val Arg Arg 35 40 45Ala Ser Ser Ala Glu Leu Gln Leu Pro Trp Thr Cys Pro Val Thr His 50 55 60Ser Arg Glu Lys Phe Tyr Thr Val Cys Ser Asp Tyr Ala Leu Leu Asn65 70 75 80Arg Ala Arg Pro Val Ile Thr Ser Glu Asp Ala Ser Gln Thr Asn Pro 85 90 95Asp Ser Gly Thr Ser Leu Ala Lys Ser Asn Thr Ala Thr Ser Ser Gln 100 105 110Ser His Ser Gly Gly Ile Ser Val Ser Leu Asp Gly Asn Cys Asp Met 115 120 125Glu Val Val Ser Ser Ser Asn Lys Pro Val Leu Ala Trp Glu Ile Asp 130 135 140Thr Ser Asp Phe Asp Ala Val Leu Thr Arg Lys Ala Arg Thr Ser Asn145 150 155 160Leu Lys Lys Phe Asn Thr Lys Lys Met Lys Ser Ser Asp Arg Pro Ser 165 170 175Arg Asn Leu Gln Asp Val Pro Pro Gln Ala Ser Leu Asp Glu Ile Lys 180 185 190Gln Arg Lys Val Leu Asp Leu Arg Arg Trp Tyr Cys Ile Ser Arg Pro 195 200 205Gln Tyr Lys Thr Ser Cys Gly Ile Ser Ser Leu Val Ser Cys Trp Asn 210 215 220Phe Leu Tyr Ser Thr Leu Gly Ala Gly Ser Leu Pro Pro Ile Ser Gln225 230 235 240Glu Glu Ala Leu His Ile Leu Gly Phe Gln Pro Pro Phe Glu Asp Ile 245 250 255Lys Phe Gly Pro Phe Thr Gly Asn Ala Thr Leu Met Arg Trp Phe Arg 260 265 270Gln Ile Asn Asp Asn Phe Arg Val Arg Gly Cys Ser Tyr Ile Leu Tyr 275 280 285Lys Pro His Gly Lys His Lys Thr Ala Gly Glu Thr Ala Glu Gly Ala 290 295 300Leu Met Lys Leu Thr Gln Gly Leu Lys Asp Glu Ser Met Ala Tyr Ile305 310 315 320Tyr His Cys Gln Asn His Tyr Phe Cys Pro Val Gly Tyr Glu Ala Thr 325 330 335Pro Leu Lys Ala Ala Lys Ala Tyr Arg Gly Pro Leu Pro Leu Asn Glu 340 345 350Met Glu His Trp Ile Leu Ile Gly Glu Pro Ser Arg Lys His Pro Ala 355 360 365Ile His Cys Lys Lys Trp Ala Asp Ile Val Thr Asp Leu Asn Thr Gln 370 375 380Asn Pro Glu Tyr Leu Asp Ile Arg His Ile Glu Arg Gly Ile Gln Tyr385 390 395 400Arg Lys Thr Lys Lys Val Gly Gly Asn Leu His Cys Ile Met Ala Phe 405 410 415Gln Arg Val Asn Trp Gln Lys Leu Gly Pro Trp Ala Leu Asn Leu Glu 420 425 430Asn Leu Arg His Asp Leu His His Gln Ala Pro Glu His Arg Gly Gln 435 440 445Ala Ser Thr Glu Asp Ser Ser Glu Glu Arg Thr Val Lys Arg Leu Gly 450 455 460Arg Ser Leu Ser Thr Gly Asn Lys Pro Glu Asn Ala Trp Lys Arg Leu465 470 475 480Ser Asn Thr Ala Glu Tyr Arg His Arg Gly Ser Pro Asp Ser Asp Leu 485 490 495Asp Glu Asp Ile Thr Asp 500122062DNAStrongylocentrotus purpuratusmisc_feature(164)..(166)translation initiation codon (ATG) 12gcagtttgtg tgtgattctc aatctcattg tgcgcattat aggcctatag ctttcgggaa 60aacagacaga ttagtgcttt gcaaaccttg catacattga ggcaaccaga aagttgggct 120caactttcag ataacctttg acctttcctg ggcctgaggt ataatgggta actggccttc 180agttctctct ggtgagggaa gtgaggacag cagcagcgag agcaacaacg aaagcaacaa 240ccaggaaacc agtgatcagg aaaacacaag acatcatctc tgtggctcag aggagagcta 300cttctccgag gaggaactcc ttcccattgt ctaccctgat gatgatgatg atgctgctgc 360tcgtgatgac gtgttgggag acttcttgtc cgttaaagaa gatggagagt ttacaactga 420cgaggttgat gggtctcgat atgacctagc acccgagtat taccccacct ctcttcatga 480agacgtcact gcgagattct cagatcttgc ctcacctgta gatcgcaaag aaagcagcta 540cagcagcact gacgactatg atgacaatga cagtgatgat gaggaggagg aggaggatga 600ccactattac caaagaagga ggaatgataa atattcccta atgaaggaag acgatgatga 660taatgagctc tccagcattc cactgccacc tccctcatca ctgtatgaag ttgcatcagc 720tgagcagatg caaggggtca cagcttacct gaatgctgac cgacctgaca cactccaaga 780aaccatcgtc ccttttgaga gtcgtgcaga agagtgcagt gcccctgaga gggtggttgc 840atgggagata gacgtcagcg acatgacggg atccaagaag actaagaaga gaccacccaa 900taaactttca aaggcaaaat caaggaaaag ttcatcgaaa ggtagcatgg atagtgccta 960tatcccgcca actgtatcaa caacacctga gctcctagca cagagaaagt gcttggacca 1020aaagagatgg ttttgtgtga gtagacccca gtacagcaag tcatgtggcc tatcgtcctt 1080ggtttcttgc tggaactacc tgttcagtac cctaggaggg ggcaccatgc cccccatcac 1140ccaggagcaa gcccttaacg tcctggggtt ccaaccaccc ttcggtgaga tccgttttgg 1200gcctttcaca gggaatgcca ccctcatgag gtggttcaag cagctgaatg atcactacag 1260agtgagagga agggcatact tccagtacaa accccatggc aggagtagaa cagtgggaag 1320aacatctgcc caaggtttac atctgttacg acaagggttg aaggatccta acatggcttt 1380catataccac tgccataacc actacttctg ccccattgga tacgaagatg tgcctctgaa 1440ggctgtagat gcatacaggg atcctttaaa ccttgatgag gtagagacat ggatactgat 1500cggtgatcct agtagaaagc aaccaggaat ccactgtttc aaatgggaag acatcagcac 1560agatctgaac tgccagaacc ctgactatct caacatccgc aagctacggc ttggagtgca 1620gcagaggagg acaaagagaa ccggtggcaa cttgcactgc atcatggcct tctgtcgcag 1680tgcaggcttt ctcaccagac caaccaagag caagaaagag ggtgcaatga aggacacttc 1740tagtaacagc aagagtagga agtctggctc cgttcggatg tcaggacgta aggttggcga 1800gagtaagagt gaggggatgg tggggcgtcc agctccagga gggagtgtgc catgtctgca 1860gactggcaaa gcggacagta gcgatatcat cgagcacttt gcttttgaga ctgtgagttg 1920cgaccatagc agtgagggcc gaagctgtag atcagaagtt gttaaaaaga ctaaaagtga 1980atctcaggtt ggcagacgaa gggcaaaggc atctgttgta aagcaggagg ataaggagat 2040cagagtgaag agttctgagg ca 206213633PRTStrongylocentrotus purpuratus 13Met Gly Asn Trp Pro Ser Val Leu Ser Gly Glu Gly Ser Glu Asp Ser1 5 10 15Ser Ser Glu Ser Asn Asn Glu Ser Asn Asn Gln Glu Thr Ser Asp Gln 20 25 30Glu Asn Thr Arg His His Leu Cys Gly Ser Glu Glu Ser Tyr Phe Ser 35 40 45Glu Glu Glu Leu Leu Pro Ile Val Tyr Pro Asp Asp Asp Asp Asp Ala 50 55 60Ala Ala Arg Asp Asp Val Leu Gly Asp Phe Leu Ser Val Lys Glu Asp65 70 75 80Gly Glu Phe Thr Thr Asp Glu Val Asp Gly Ser Arg Tyr Asp Leu Ala 85 90 95Pro Glu Tyr Tyr Pro Thr Ser Leu His Glu Asp Val Thr Ala Arg Phe 100 105 110Ser Asp Leu Ala Ser Pro Val Asp Arg Lys Glu Ser Ser Tyr Ser Ser 115 120 125Thr Asp Asp Tyr Asp Asp Asn Asp Ser Asp Asp Glu Glu Glu Glu Glu 130 135 140Asp Asp His Tyr Tyr Gln Arg Arg Arg Asn Asp Lys Tyr Ser Leu Met145 150 155 160Lys Glu Asp Asp Asp Asp Asn Glu Leu Ser Ser Ile Pro Leu Pro Pro 165 170 175Pro Ser Ser Leu Tyr Glu Val Ala Ser Ala Glu Gln Met Gln Gly Val 180 185 190Thr Ala Tyr Leu Asn Ala Asp Arg Pro Asp Thr Leu Gln Glu Thr Ile 195 200 205Val Pro Phe Glu Ser Arg Ala Glu Glu Cys Ser Ala Pro Glu Arg Val 210 215 220Val Ala Trp Glu Ile Asp Val Ser Asp Met Thr Gly Ser Lys Lys Thr225 230 235 240Lys Lys Arg Pro Pro Asn Lys Leu Ser Lys Ala Lys Ser Arg Lys Ser 245 250 255Ser Ser Lys Gly Ser Met Asp Ser Ala Tyr Ile Pro Pro Thr Val Ser 260 265 270Thr Thr Pro Glu Leu Leu Ala Gln Arg Lys Cys Leu Asp Gln Lys Arg 275 280 285Trp Phe Cys Val Ser Arg Pro Gln Tyr Ser Lys Ser Cys Gly Leu Ser 290 295 300Ser Leu Val Ser Cys Trp Asn Tyr Leu Phe Ser Thr Leu Gly Gly Gly305 310 315 320Thr Met Pro Pro Ile Thr Gln Glu Gln Ala Leu Asn Val Leu Gly Phe 325 330 335Gln Pro Pro Phe Gly Glu Ile Arg Phe Gly Pro Phe Thr Gly Asn Ala 340 345 350Thr Leu Met Arg Trp Phe Lys Gln Leu Asn Asp His Tyr Arg Val Arg 355 360 365Gly Arg Ala Tyr Phe Gln Tyr Lys Pro His Gly Arg Ser Arg Thr Val 370 375 380Gly Arg Thr Ser Ala Gln Gly Leu His Leu Leu Arg Gln Gly Leu Lys385 390 395 400Asp Pro Asn Met Ala Phe Ile Tyr His Cys His Asn His Tyr Phe Cys 405 410 415Pro Ile Gly Tyr Glu Asp Val Pro Leu Lys Ala Val Asp Ala Tyr Arg 420 425 430Asp Pro Leu Asn Leu Asp Glu Val Glu Thr Trp Ile Leu Ile Gly Asp 435 440 445Pro Ser Arg Lys Gln Pro Gly Ile His Cys Phe Lys Trp Glu Asp Ile 450 455 460Ser Thr Asp Leu Asn Cys Gln Asn Pro Asp Tyr Leu Asn Ile Arg Lys465 470 475 480Leu Arg Leu Gly Val Gln Gln Arg Arg Thr Lys Arg Thr Gly Gly Asn 485 490 495Leu His Cys Ile Met Ala Phe Cys Arg Ser Ala Gly Phe Leu Thr Arg 500 505 510Pro Thr Lys Ser Lys Lys Glu Gly Ala Met Lys Asp Thr Ser Ser Asn 515 520 525Ser Lys Ser Arg Lys Ser Gly Ser Val Arg Met Ser Gly Arg Lys Val 530 535 540Gly Glu Ser Lys Ser Glu Gly Met Val Gly Arg Pro Ala Pro Gly Gly545 550 555 560Ser Val Pro Cys Leu Gln Thr Gly Lys Ala Asp Ser Ser Asp Ile Ile 565 570 575Glu His Phe Ala Phe Glu Thr Val Ser Cys Asp His Ser Ser Glu Gly 580 585 590Arg Ser Cys Arg Ser Glu Val Val Lys Lys Thr Lys Ser Glu Ser Gln 595 600 605Val Gly Arg Arg Arg Ala Lys Ala Ser Val Val Lys Gln Glu Asp Lys 610 615 620Glu Ile Arg Val Lys Ser Ser Glu Ala625 630142031DNAGiardia lambliamisc_feature(230)..(232)Translation initiation codon (ATG) 14gcacatcttg caggtcaaaa cgaacacccc ctccttcgat atcctctcag accctacact 60ctcaattgtg ttacagaccg ggcatgggaa gaacttgcta cggccggctt tcttaggggg 120cgccgccctt gtcctcttct tctttcccat cctcctgtcc tctttttgtg actgtttgtg 180actagacgcc gtttctaaca aaattgccaa gcatgtatgc aaaattaaaa tggaaagata 240ccccagacaa cggttagacg acggcaggtg gcagtgcgtg gcagcgcagt acagatactc 300ctgcgccatc tcatgccttg tgagcatatt caatcatctc ttcaacagag acatgaccct 360ggacgagtgt attgctattc tctttccaga cctgaaagaa gacccacgac actatgattt 420tggacctcag gcttctaaca gtgctgttca aagctggttc aagaccctct gcatgcacta 480tggcctttct ggcacctctt gcacgatata caaggagcag ggcagaacga gaactgcgtg 540tagcaagcaa gaggcactta agaatatcat cactgctttg aatacgccaa gatgtgcgtt 600actgtatcac tgcttgaacc attactgcat aatcgtaggc tatataataa gtccatctac 660gcctaataga ccaagtaatc attgcgtctt cagcggggat gatggatgca ccctcaagct 720cctgtgtgca gacggcacag aagccgagga cgtggacgat agtaatattt ggttaatagt 780ggcagactgt gggaaaggaa ctgctcccct taggtcactg acctgggaat ttgtacataa 840agatatatct acccgacctc cgtatgcata taacgctagg tgccctgaga gaggactgct 900aaggaaaaca gaatcaaagg gatatatacc agttgagata gactcagtgc ttgttaacag 960cacgggagta tccacctgtg ttagatctgg tggcgtcatc aagggatcgt cgcactgcat 1020cattggattt gttagtgact agagccccgt ttattactcc cggacgaaag tataactatt 1080aacaccacaa gcacaacgat agctccagta gagcagagcc gaagcacttg aggcagcgag 1140gcctccaaat acccacatag aacgtcacag atgatagctg tccatgtcgc aattgacaag 1200gttaacggga aggttgaaac aggcgagggc gtccatctgg tacgttgtac tttggttgtt 1260gaatattgaa ctgttgtaag tgttgatttg ctgggtatat ctattgctta tgtaccgaaa 1320aagggcattg caaacgtcat atattgcatc tatctgatga acacagaccc cagttttttg 1380aagatttgca agtcttcttt gtggtggggc attcatatat gaataagagc agacttctcc 1440gcaggcaaag gacatggact gaatggcatg ctcgtaacca gttaggtcca gtgctttggt 1500tcgtgcatag tatttaaaga ccttctgaag aaggatggtt tgaaataggg tcgtcctgtc 1560cacacagtcc aggcagttta tccgcggata gcacttctga acaaagtcag gaagagcaac 1620tccgacatca ccgctaggaa ctagaactgt gcttgtggct atgtcatctg ctaactggtg 1680atactctgtg ttgctgtgtc tacgtatgtt gtagttcatc aacttaacgt tgagggagtt 1740cttgcggcga gaatcagcag tttttctcat agactcggta aagaacgccg tcagagccgc 1800tcatcggcgg tctcaaggct tttcttttca ctggcagcaa tggagtcatc caaaagatcg 1860acttcatttt tgaggaggtt gacgataagt atctctgcgt ctgcagtcac taagttaccc 1920aatagaaggc ttatatgcct ttgcaagaga ctactaaact gagcgaggcc ctgctcttca 1980tgagccccat ctgggaagcg tatggcagga gtgaacttgt aagtaaaaaa a 203115270PRTGiardia lamblia 15Met Glu Arg Tyr Pro Arg Gln Arg Leu Asp Asp Gly Arg Trp Gln Cys1 5 10 15Val Ala Ala Gln Tyr Arg Tyr Ser Cys Ala Ile Ser Cys Leu Val Ser 20 25 30Ile Phe Asn His Leu Phe Asn Arg Asp Met Thr Leu Asp Glu Cys Ile 35 40 45Ala Ile Leu Phe Pro Asp Leu Lys Glu Asp Pro Arg His Tyr Asp Phe 50 55 60Gly Pro Gln Ala Ser Asn Ser Ala Val Gln Ser Trp Phe Lys Thr Leu65 70

75 80Cys Met His Tyr Gly Leu Ser Gly Thr Ser Cys Thr Ile Tyr Lys Glu 85 90 95Gln Gly Arg Thr Arg Thr Ala Cys Ser Lys Gln Glu Ala Leu Lys Asn 100 105 110Ile Ile Thr Ala Leu Asn Thr Pro Arg Cys Ala Leu Leu Tyr His Cys 115 120 125Leu Asn His Tyr Cys Ile Ile Val Gly Tyr Ile Ile Ser Pro Ser Thr 130 135 140Pro Asn Arg Pro Ser Asn His Cys Val Phe Ser Gly Asp Asp Gly Cys145 150 155 160Thr Leu Lys Leu Leu Cys Ala Asp Gly Thr Glu Ala Glu Asp Val Asp 165 170 175Asp Ser Asn Ile Trp Leu Ile Val Ala Asp Cys Gly Lys Gly Thr Ala 180 185 190Pro Leu Arg Ser Leu Thr Trp Glu Phe Val His Lys Asp Ile Ser Thr 195 200 205Arg Pro Pro Tyr Ala Tyr Asn Ala Arg Cys Pro Glu Arg Gly Leu Leu 210 215 220Arg Lys Thr Glu Ser Lys Gly Tyr Ile Pro Val Glu Ile Asp Ser Val225 230 235 240Leu Val Asn Ser Thr Gly Val Ser Thr Cys Val Arg Ser Gly Gly Val 245 250 255Ile Lys Gly Ser Ser His Cys Ile Ile Gly Phe Val Ser Asp 260 265 27016196DNABranchiostoma floridae 16tgatttgtgg ttccggcaga tcaatgatca tttccatgta aaaggatgct cctatgttct 60gtataagccg catggcaaga acaagacagc aggagaaact gctgttgggg cactatcaga 120gttaacacaa gggttaaaag aagacccaac agcctacgtc tatcattgcc agaaccacta 180cttctgcccc aatccc 1961765PRTBranchiostoma floridae 17Asp Leu Trp Phe Arg Gln Ile Asn Asp His Phe His Val Lys Gly Cys1 5 10 15Ser Tyr Val Leu Tyr Lys Pro His Gly Lys Asn Lys Thr Ala Gly Glu 20 25 30Thr Ala Val Gly Ala Leu Ser Glu Leu Thr Gln Gly Leu Lys Glu Asp 35 40 45Pro Thr Ala Tyr Val Tyr His Cys Gln Asn His Tyr Phe Cys Pro Asn 50 55 60Pro6518382DNAMus musculus5'UTR(1)..(382)Exon A - untranslated 18ccccaactac tttcgtccct tccctccgtc cctcactctc cctcctcctt tctccccccc 60taccttcctt tctacttctt ttttcaactt tggagcacgg ctttctggca accttaaata 120ctacagttgc gcaactagca tgtctggagt cacagcaaag atttcccaac ttatattttg 180ttcaaggtat ccaccgcaaa tggcaggtat atagtaaacg ctgaaaggga ggctaggtgt 240tatcaatgat acccagtcac tcggtgctat tcttgtgcgc tcaatgggac gaaagattct 300gggccttggg taggagactt ggagatgcaa gatctggtgt tgccttccag caccagagtt 360ccgggaccca acaggaacag ag 3821942DNAMus musculus5'UTR(1)..(42)Exon B - untranslated 19ccctggaagg atctgggtcg agctgagtct ctgaggagag at 4220311DNAMus musculus5'UTR(1)..(311)Exon C - untranslated 20ttttcttccg gctgggagtg agggagcagg ccgggaggag gttacaaggc tttagatctg 60gtcttggcca gtggggacta gggacgcctg gcactgggtt ggccaccgca ggacagtagt 120gggaacccgg cacagtagcg ctgcagcagt tgcacttgca acatccctgc tctcccggtt 180ctcctccacc tgcacctttg tcaccttcag gtgcttcgga gcctcaaaga gggggcagtg 240ggaagtctcc tggctcctca gagtctgaac tccagagggc atcatgtgct gcatgaatct 300catactcaca g 31121601DNAMus musculus5'UTR(1)..(125)Exon 1 - untranslated 21gatcccattt gtcagctctc aagccttttt agaatcctgt gaacatttgc caaagttgct 60tttttttttt ttaaagagag ggttgcggct tcttcctagg aacagagaca tctgcatttg 120ctctcatgcc taacgccact gaagctggaa aagccactga tcctggacat ggtgagcaca 180catctgagaa caagtcacca gaagagggtc tacaaggtgc tgtaccatct ttctacacaa 240gtgcctcaga agcacccata gcgcccagag gagatgggca ttatccatcg agttgtccag 300tgactcacac tcgagagaaa atttatgcga tctgctcaga ttatgccttc ctcaaccagg 360caacatcagt ctacaaaact cctagcctaa cccgctctgc ttgcctccct gataacacct 420ctctttctgc tggaaatact acaagatata ttggaatttc aactagtaca tcagaaataa 480tctataatga aggaaaataa cttggaaaac ttgtccactg gcatgggcaa gctacctctt 540gcatgggaga ttgataaatc tgaatttgat ggggtgacta caaatttgat acataagtca 600g 60122912DNAMus musculusmisc_feature(1)..(912)Alternative BIVM 5' end clone (6359) 22atattttgtt caaggtatcc accgcaaatg gcaggtatat agtaaacgct gaaagggagg 60ctaggtgtta tcaatgatac ccagtcactc ggtgctattc ttgtgcgctc aatgggacga 120aagattctgg gccttgggta ggagacttgg agatgcaaga tctggtgttg ccttccagca 180ccagagttcc gggacccaac aggaacagag gtgcttcgga gcctcaaaga gggggcagtg 240ggaagtctcc tggctcctca gagtctgaac tccagagggc atcatgtgct gcatgaatct 300catactcaca ggatcccatt tgtcagctct caagcctttt tagaatcctg tgaacatttg 360ccaaagttgc tttttttttt tttaaagaga gggttgcggc ttcttcctag gaacagagac 420atctgcattt gctctcatgc ctaacgccac tgaagctgga aaagccactg atcctggaca 480tggtgagcac acatctgaga acaagtcacc agaagagggt ctacaaggtg ctgtaccatc 540tttctacaca agtgcctcag aagcacccat agcgcccaga ggagatgggc attatccatc 600gagttgtcca gtgactcaca ctcgagagaa aatttatgcg atctgctcag attatgcctt 660cctcaaccag gcaacatcag tctacaaaac tcctagccta acccgctctg cttgcctccc 720tgataacacc tctctttctg ctggaaatac tacaagatat attggaattt caactagtac 780atcagaaata atctataatg aaggaaaata acttggaaaa cttgtccact ggcatgggca 840agctacctct tgcatgggag attgataaat ctgaatttga tggggtgact acaaatttga 900tacataagtc ag 91223912DNAMus musculusmisc_feature(1)..(912)Alternative BIVM 5' end clone (6358) 23ttttcttccg gctgggagtg agggagcagg ccgggaggag gttacaaggc tttagatctg 60gtcttggcca gtggggacta gggacgcctg gcactgggtt ggccaccgca ggacagtagt 120gggaacccgg cacagtagcg ctgcagcagt tgcacttgca acatccctgc tctcccggtt 180ctcctccacc tgcacctttg tcaccttcag gtgcttcgga gcctcaaaga gggggcagtg 240ggaagtctcc tggctcctca gagtctgaac tccagagggc atcatgtgct gcatgaatct 300catactcaca ggatcccatt tgtcagctct caagcctttt tagaatcctg tgaacatttg 360ccaaagttgc tttttttttt tttaaagaga gggttgcggc ttcttcctag gaacagagac 420atctgcattt gctctcatgc ctaacgccac tgaagctgga aaagccactg atcctggaca 480tggtgagcac acatctgaga acaagtcacc agaagagggt ctacaaggtg ctgtaccatc 540tttctacaca agtgcctcag aagcacccat agcgcccaga ggagatgggc attatccatc 600gagttgtcca gtgactcaca ctcgagagaa aatttatgcg atctgctcag attatgcctt 660cctcaaccag gcaacatcag tctacaaaac tcctagccta acccgctctg cttgcctccc 720tgataacacc tctctttctg ctggaaatac tacaagatat attggaattt caactagtac 780atcagaaata atctataatg aaggaaaata acttggaaaa cttgtccact ggcatgggca 840agctacctct tgcatgggag attgataaat ctgaatttga tggggtgact acaaatttga 900tacataagtc ag 91224888DNAMus musculusmisc_feature(1)..(888)Alternative BIVM 5' end clone (6356) 24ccccaactac tttcgtccct tccctccgtc cctcactctc cctcctcctt tctccccccc 60taccttcctt tctacttctt ttttcaactt tggagcacgg ctttctggca accttaaata 120ctacagttgc gcaactagca tgtctggagt cacagcaaag atttcccaac ttatattttg 180ttcaaggtgc ttcggagcct caaagagggg gcagtgggaa gtctcctggc tcctcagagt 240ctgaactcca gagggcatca tgtgctgcat gaatctcata ctcacaggat cccatttgtc 300agctctcaag cctttttaga atcctgtgaa catttgccaa agttgctttt ttttttttta 360aagagagggt tgcggcttct tcctaggaac agagacatct gcatttgctc tcatgcctaa 420cgccactgaa gctggaaaag ccactgatcc tggacatggt gagcacacat ctgagaacaa 480gtcaccagaa gagggtctac aaggtgctgt accatctttc tacacaagtg cctcagaagc 540acccatagcg cccagaggag atgggcatta tccatcgagt tgtccagtga ctcacactcg 600agagaaaatt tatgcgatct gctcagatta tgccttcctc aaccaggcaa catcagtcta 660caaaactcct agcctaaccc gctctgcttg cctccctgat aacacctctc tttctgctgg 720aaatactaca agatatattg gaatttcaac tagtacatca gaaataatct ataatgaagg 780aaaataactt ggaaaacttg tccactggca tgggcaagct acctcttgca tgggagattg 840ataaatctga atttgatggg gtgactacaa atttgataca taagtcag 88825668DNAMus musculusmisc_feature(1)..(668)Alternative BIVM 5' end clone (cDNA) 25ccctggaagg atctgggtcg agctgagtct ctgaggagag atgtgcttcg gagcctcaaa 60gagggggcag tgggaagtct cctggctcct cagagtctga actccagagg gcatcatgtg 120ctgcatgaat ctcatactca cagagagggt tgcggcttct tcctaggaac agagacatct 180gcatttgctc tcatgcctaa cgccactgaa gctggaaaag ccactgatcc tggacatggt 240gagcacacat ctgagaacaa gtcaccagaa gagggtctac aaggtgctgt accatctttc 300tacacaagtg cctcagaagc acccatagcg cccagaggag atgggcatta tccatcgagt 360tgtccagtga ctcacactcg agagaaaatt tatgcgatct gctcagatta tgccttcctc 420aaccaggcaa catcagtcta caaaactcct agcctaaccc gctctgcttg cctccctgat 480aacacctctc tttctgctgg aaatactaca agatatattg gaatttcaac tagtacatca 540gaaataatct ataatgaagg aaaataactt ggaaaacttg tccactggca tgggcaagct 600acctcttgca tgggagattg ataaatctga atttgatggg gtgactacaa atttgataca 660taagtcag 668263312DNAMus musculusmisc_feature(1)..(209)Exon A 26atattttgtt caaggtatcc accgcaaatg gcaggtatat agtaaacgct gaaagggagg 60ctaggtgtta tcaatgatac ccagtcactc ggtgctattc ttgtgcgctc aatgggacga 120aagattctgg gccttgggta ggagacttga ggatgcagat ctggtgttgc cttccagcac 180cagagttccg ggacccaaca ggaacagagg tgcttcggag cctcaaagag gggcagtggg 240aagtctcctg gctcctcaga gtctgaactc cagagggcat catgtgctgc atgaatctca 300tactcacagg atcccatttg tcagctctca agccttttta gaatcctgtg aacatttgcc 360aaagttgctt tttttttttt tttaaagaga gggttgcggc ttcttcctag gaacagagac 420atctgcattt gctctcatgc ctaacgccac tgaagctgga aaagccactg atcctggaca 480tggtgagcac acatctgaga acaagtcacc agaagagggt ctacaaggtg ctgtaccatc 540tttctataca agtgcctcag aagcacccat agcgcccaga ggagatgggc attatccatc 600gagttgtcca gtgactcaca ctcgagagaa aatttatgcg atctgctcag attatgcctt 660cctcaaccag gcaacatcag tctacaaaac tcctagccta acccgctctg cttgcctccc 720tgataacacc tctctttctg ctggaaatac tacaagatat attggaattt caactagtac 780atcagaaata atctataatg aagaaaataa cttggaaaac ttgtccactg gcatgggcaa 840gctacctctt gcatgggaga ttgataaatc tgaatttgat ggggtgacta caaatttgat 900acataagtca ggcaatgtaa agaaacaatt ttccaagaag aaaacgtcgg ataaaaaagg 960gcggcatcag agggagtgtc tccactattc tcctcttgat gatgttaaac aacgcaaagt 1020gttagacctt aggcgatggt actgcataag ccgaccacag tacaagactt catgtggtat 1080ctcctcattg atttcttgtt ggaatttctt atacagcata atgggagctg ggaatctccc 1140acctattacc caagaagagg cattacatat tttgggcttc caacccccat ttgaagatat 1200taggtttggc cctttcactg gaaatacaac actcatgaga tggtttagac aaattaatga 1260ccactttcat gtgaaaggat gctcttatgt tctatataag ccccatggga agaacaaaac 1320agctggagaa actgctccag gggccttatc aaagttgacc cgaggattga aagatgagtc 1380actggcttat atctatcatt gccaaaatca ctatttctgt ccaattggct ttgaagcaac 1440ccctgtgaaa gctaataaag cattcagcag ggggcccctc tcttcacaag aagtagaata 1500ctggatttta attggagagt caagtagaaa acatcctgcc attcactgta aaagatgggc 1560agatattgtc actgatctaa acactcaaaa tccagaattc ttagatatcc gacatctaga 1620gagggggctg cagttccgga aaataaagaa ggttggagga aatttgcatt gcatcatagc 1680attccagaga ctcagttggc agagatttgg cttttggaac tttccatttg gaaccattac 1740acaagaatca caacatccca cacatgtccc gggaattgcc aaatctgaga gtgaggacaa 1800tatctctaag aagcagcatg ggcgcctggg caggtccttc agtgcgagtt tccatcagga 1860ctcggcatgg aagaacatgt ctagcatcca cgagaggagg aacagtggct accacagctt 1920tagagattat aatggcaatg actgaccatg ccaaaactta gccactggtg ttacccacac 1980agctgttatg tacaggactg cattaggaca tcagctggtt ttattaagtc tgtcaatagg 2040aacagatttt gtggtacaaa acacaccctg tagttctcta gtaaaaaagc ctacatagga 2100ttactatggt tggcttcaaa tatacaggca ggtaagcaca gaaccccgcc cttctaaagt 2160taaaagtaga taagcaatct ggacaaaggg tttcacaaaa tccaatacaa tcaaaacggc 2220ttcaaagcaa aaacacaaat gcatttaatt tgaaaagcat cgaaacttga actacttaag 2280catgaagcga cttattgata cttgatccct agcatttatt acaacacttt aattcctaag 2340gcatcatctg tccttaaaaa atgggggcag tcaaggtcta gtttttgctc atggttaaaa 2400ctaatttaaa attatctttc tagtctagtt gttctttcag tgctaacagt atccacctcc 2460catcgttgct ttcctgaata actctcagga ttctccaaaa agcagcagaa actactccag 2520gaactgacct tttctctagg tgcagatagg tgacttaggt cattgatcct gatactcttg 2580acttggcacg tggttgtgaa atagctacaa gaagaatata ggtctggagc gaagtctgat 2640gttctagaac aaaccttgtt tcagggatat agttagagag cacttggcat ccaaagtttc 2700cttatccacg gtaacatgtg ctgtgagatg tcacatttga cttgtctctt aatggagtca 2760tgtgttaaca acagcactga tgtcatgttg gcaatgtcca gctcactctg aggaagactt 2820tgtattttca actctgagcc gtttcctttt gtgaaacctc caagcaatta ggtgttggaa 2880gtgtgagtta catattctgg aagtgtgagt tcaatacttg agctcctctt tagcggctct 2940tgttttcctt ttgctgccaa ggtgtgactc atagccgtct atgatgctgc tctttcacgt 3000cgtaggttta ttccaggatt caaatcagta acttggtgat tacaaggtgc tgagtatgtt 3060ggaaccattg caatacacct caaagggagg tgtcggattt tgacttttta aaaaaaattt 3120tcatttttct cttgaatttc atatccatct atccactcat atatgtttag cctacagaat 3180tacaaactag tcctgtttct gaagaggttc tttagcttga aatgtaaagg actgaaagat 3240ttgtaggtgt tcttttgtta cttcacactg gaactttgaa aatgttttca tcaaataaag 3300ttttgttttc ta 331227502PRTMus musculus 27Met Pro Asn Ala Thr Glu Ala Gly Lys Ala Thr Asp Pro Gly His Gly1 5 10 15Glu His Thr Ser Glu Asn Lys Ser Pro Glu Glu Gly Leu Gln Gly Ala 20 25 30Val Pro Ser Phe Tyr Thr Ser Ala Ser Glu Ala Pro Ile Ala Pro Arg 35 40 45Gly Asp Gly His Tyr Pro Ser Ser Cys Pro Val Thr His Thr Arg Glu 50 55 60Lys Ile Tyr Ala Ile Cys Ser Asp Tyr Ala Phe Leu Asn Gln Ala Thr65 70 75 80Ser Val Tyr Lys Thr Pro Ser Leu Thr Arg Ser Ala Cys Leu Pro Asp 85 90 95Asn Thr Ser Leu Ser Ala Gly Asn Thr Thr Arg Tyr Ile Gly Ile Ser 100 105 110Thr Ser Thr Ser Glu Ile Ile Tyr Asn Glu Glu Asn Asn Leu Glu Asn 115 120 125Leu Ser Thr Gly Met Gly Lys Leu Pro Leu Ala Trp Glu Ile Asp Lys 130 135 140Ser Glu Phe Asp Gly Val Thr Thr Asn Leu Ile His Lys Ser Gly Asn145 150 155 160Val Lys Lys Gln Phe Ser Lys Lys Lys Thr Ser Asp Lys Lys Gly Arg 165 170 175His Gln Arg Glu Cys Leu His Tyr Ser Pro Leu Asp Asp Val Lys Gln 180 185 190Arg Lys Val Leu Asp Leu Arg Arg Trp Tyr Cys Ile Ser Arg Pro Gln 195 200 205Tyr Lys Thr Ser Cys Gly Ile Ser Ser Leu Ile Ser Cys Trp Asn Phe 210 215 220Leu Tyr Ser Ile Met Gly Ala Gly Asn Leu Pro Pro Ile Thr Gln Glu225 230 235 240Glu Ala Leu His Ile Leu Gly Phe Gln Pro Pro Phe Glu Asp Ile Arg 245 250 255Phe Gly Pro Phe Thr Gly Asn Thr Thr Leu Met Arg Trp Phe Arg Gln 260 265 270Ile Asn Asp His Phe His Val Lys Gly Cys Ser Tyr Val Leu Tyr Lys 275 280 285Pro His Gly Lys Asn Lys Thr Ala Gly Glu Thr Ala Pro Gly Ala Leu 290 295 300Ser Lys Leu Thr Arg Gly Leu Lys Asp Glu Ser Leu Ala Tyr Ile Tyr305 310 315 320His Cys Gln Asn His Tyr Phe Cys Pro Ile Gly Phe Glu Ala Thr Pro 325 330 335Val Lys Ala Asn Lys Ala Phe Ser Arg Gly Pro Leu Ser Ser Gln Glu 340 345 350Val Glu Tyr Trp Ile Leu Ile Gly Glu Ser Ser Arg Lys His Pro Ala 355 360 365Ile His Cys Lys Arg Trp Ala Asp Ile Val Thr Asp Leu Asn Thr Gln 370 375 380Asn Pro Glu Phe Leu Asp Ile Arg His Leu Glu Arg Gly Leu Gln Phe385 390 395 400Arg Lys Ile Lys Lys Val Gly Gly Asn Leu His Cys Ile Ile Ala Phe 405 410 415Gln Arg Leu Ser Trp Gln Arg Phe Gly Phe Trp Asn Phe Pro Phe Gly 420 425 430Thr Ile Thr Gln Glu Ser Gln His Pro Thr His Val Pro Gly Ile Ala 435 440 445Lys Ser Glu Ser Glu Asp Asn Ile Ser Lys Lys Gln His Gly Arg Leu 450 455 460Gly Arg Ser Phe Ser Ala Ser Phe His Gln Asp Ser Ala Trp Lys Asn465 470 475 480Met Ser Ser Ile His Glu Arg Arg Asn Ser Gly Tyr His Ser Phe Arg 485 490 495Asp Tyr Asn Gly Asn Asp 5002834562DNAMus musculusmisc_feature(349)..(554)KDEL exon 28ccagacttgg tcctttgcaa gtgcttttca ctgccgagcc atctctctag cttttttttt 60tttttttttt ctctctagct ttttaagttg attattctca caggtgtgtc ctcaaggagc 120aacttttcta gtaatgtttc ttggtcttta gtgaattata atccccatgt aaataatcaa 180tatctctctt ttgccaagtt gcctaattta gttttcaatt agaaaaacat ctagtttttt 240tttccattta gtgaaaaaag tatgctaagt ttttgataga attcttattg atggaatgac 300atgccttttt gtatacaaat cctaccataa atttttgttt caaaatacct cttaaaacat 360aaggagactc ggcaacatgt tgcccatggt gtttaacctc tatcttcaga tttctgtagc 420tggcatacat tctgtatctt actatgaagg aaccgtcctt tcgatctaaa acctggacac 480caactctcgt gaactgctca tcgggtgctg agattttaac ctggaacacc ttttcacctg 540gagaagatgt aaacctgtta tggaaaacaa tgagagatga gggttatttt taagatcaaa 600tgcacgctac ttattctctt taatgtgtaa aggttggacc ttctgcttta tcctgatttt 660aagatattgt tgttcgatta ctctcttagg cttttaggac cagaaccaat atgatagaac 720aaagataaaa aagaaaacaa taataacata aaataatttc attagatagt tgccaggaga 780taacacttaa gactagcaat ctatttagca taccaaatag agcactatca gtgtactatt 840attaattcat aaccaacaca gctggcaatt tgtcatttgt aacatgttta cttcaaggga 900tgaccttatt tgaaaaattg ttcttaatgg cttaaaatac aatccagtac tgtggggcta 960gggagatagc tgaatggttt agagcattta ccattatgta aaagacttag ttcagttcct 1020agtatccatt gtccttaagg ctcacaacta tctgcagttt cagctcaagg agattcaagg

1080ccctcttctg gcttcctcca gcacttgggt gtgagtatac accctaccca catcatgtaa 1140ttaaaatatt actactaata aaagtctaac aaatatacca gtactctaaa ttatgtttag 1200ttatggggga aatcaaaata actggtcatc atgttagtaa cacaagtgtt ttggactgaa 1260tattcctgca ttttgaatgc atttagtttc tagtgtttag ttttcccatt taaaaataac 1320ggagatttgt ttagcctcaa ttcccatcta aacagccaag aagagagctg ttattttagc 1380aatattattt acactataca tatttcagaa ttattaagtc acagagaaaa gttgattttg 1440aaggaaaagt ctattataac cagatgtaga cagcactcga gaattctgga atgtaaatgg 1500acagcagtaa agacgagtcg gggcatataa agatgaagga caaaaccaaa ataaacaaca 1560ggaacaaaaa cccctcgagt tattttaaac cgcgatctca aatagttgag gagtcatttc 1620tccacttact gttcccctga ggtatccacc gcccgaatgt acgaaatagc gggcaggaag 1680gaccacgtgt gcttttagcc cgggtcccat atttcgctct tctctgggct cagctcctct 1740ctccgccggt ctgggcaagt gctggcacag tccccagaaa aaggcaggaa agcagcgaaa 1800tgctgaacat ttacaagacc gagtcttgga agatccacga ataaatgcaa acgtgctaag 1860tggacggagc tagcctgggt aggtaggagg acggtggtca gtcctagcag cagtcaaagt 1920ccctaaagtg tcgcttgacg tgcggggtcc gccgtgggac tatgctgtgc ccaagaatgt 1980cacaacccga gcggtggcat gtccaggaga aggatgccac acgttctgct gaacctggtt 2040aagaaccgca ggctccactt tctccggaca atgacaaact tgtgttactc ttgccactag 2100agcatcactt tgggaacgaa tccgtcgcaa gttctagcca aggtgagagg agaaggattg 2160gcctgcgctg tgaccagtct tagtaaagtc ttctgctagt ggatgagtgg gtcagagtgg 2220aaaacgcgtc ccgggcccct tagcttcctg ggatatgtag tccgccatag gactagcgga 2280aatcctgcca ggagttcacc cccaactact ttcgtccctt ccctccgtcc ctcactctcc 2340ctcctccttt ctccccccct accttccttt ctacttcttt tttcaacttt ggagcacggc 2400tttctggcaa ccttaaatac tacagttgcg caactagcat gtctggagtc acagcaaaga 2460tttcccaact tatattttgt tcaaggtatc caccgcaaat ggcaggtata tagtaaacgc 2520tgaaagggag gctaggtgtt atcaatgata cccagtcact cggtgctatt cttgtgcgct 2580caatgggacg aaagattctg ggccttgggt aggagacttg gagatgcaag atctggtgtt 2640gccttccagc accagagttc cgggacccaa caggaacaga ggtattcccc agcggagggc 2700ctagcccagg cactgcggtg cgctccgcct ctccgtagcg ccgtcactgc ctcgttgcag 2760acaaccccca cccccacccc caccccgccg cgcccctgga aggatctggg tcgagctgag 2820tctctgagga gagatgtaag ggatagaaat caccagaaga aagctcggcc tgaggggggt 2880gcatccgtgg gtatccctgt actctcttca ggagcgggtc ttcccvtgcg ctagcggatc 2940ccaggcaccc ggcctgcagt ggccatcctc tctttgcacc ctcgcttctc ggccagtgag 3000gtcaagagga gtagcaggct ttgtcctcct ccacggggta aggggcgtgg aaaacataga 3060cggcctggtt gtgagcccaa ggcaagggga ttctttttcc ccctgccccc cccccccgct 3120attgttatta ttggtgatat aatcattcat cgcggcctcc cctcccagct ctcaggctgt 3180cactgtctgc gtgccaccag cctctccagt ccctagcgcg taggaagcgg cccccttcag 3240gcctgcgcgc tccccctcct ggcgagcctt ttcttccggc tgggagtgag ggagcaggcc 3300gggaggaggt tacaaggctt tagatctggt cttggccagt ggggactagg gacgcctggc 3360actgggttgg ccaccgcagg acagtagtgg gaacccggca cagtagcgct gcagcagttg 3420cacttgcaac atccctgctc tcccggttct cctccacctg cacctttgtc accttcaggt 3480gcttcggagc ctcaaagagg gggcagtggg aagtctcctg gctcctcaga gtctgaactc 3540cagagggcat catgtgctgc atgaatctca tactcacagg taggcctccc aggtctggtc 3600tggtagtcta ggcaagggcg ctgatagaaa aggggggcgg tgggggcacc tggctgctgt 3660cttagcagct cttatcaatc ctcagcaaaa cacttttctg ggtctcagtt gctttctatg 3720cagaatattg attatactgt ttgtgatatg tggaaggctt tactgagatt ttattggatg 3780tctagctctc tactgaatgt gatacttcag catgtagaca cgaaaggcac ataaatggaa 3840aagaataaac tcaggcaatc gatatcggtt ttgcaaacag tatgtttatt tgacagaatt 3900gttcattcca aagactttca taagtcattc ctctagtgcc gtcctagaag cttggggttt 3960atattgctta tgagatactg tgaataaatt gagtggtatt ccgcaagtcc taatatgtta 4020atcaaaacga gggagaacaa tttcaataat gcctagtgca ttgtagacac ctaaaaatgt 4080ttgttagacc tggcttctac aagactaggc ctggagatgc aatttcagga aaactagact 4140ttcaggagtt aaccatttgt gtacacacac acacacacac acacacacac acacacattt 4200agaaagctta atcatattct agaaagaaaa gttcctcact atggataggg tatctggcct 4260agttagaaca gcaaaaaaaa aaaaaaaaat tcttcagaaa gtgacatttg agtataactt 4320tgaaagatgg atgagaatga ataattagta aggggctaca ggaagacaac attcagaaaa 4380ccaaaaagta atgtggaagg acagaagaag gtatgtaggg taaggagttg atcttttgct 4440tcaatagctg agggccttag gcagaaaatt tctatgagga tatatatgta gcagacttta 4500ctctttgatg tctatgcagg gatcagacta gaaagaacag ctcaaagggt gggtggcatg 4560aagttataag cataaagata aaagcagata taaaccccag atagctgcag atacagtgct 4620ggacaatcaa tcagccttca taactccgtt ttccctgaga ctaccattgc atcttactag 4680aataatctat gcctgtaatg gtcttgtttc cagtagttgt ggctttcttc attctaagaa 4740agcaggctct gagtgaagtc ctaaggatac catagcagaa gcccttcaga gtttaactac 4800aaagggtgga gatgcagatc agtcacggga tgcaaagaag tagagtgcca gcatgggttc 4860tgggtcattc tttttcattt tctgtgaata tcttctgata tttcataatt ttctacaata 4920tactttgtgt catcaaacct ataaatatat gtattttcag agatattcac ataaaacagt 4980ggttcaaatg aggaaatgtt gatatctttc attttcagtg acaactatgt aaaatcttca 5040ggtagatggc aggtttgttt gttttttttt aagataaaat gagagcaaag agattatttt 5100tattcagtga ccattgctta aagcatacaa tccaaaacca gatggttgca tgttttataa 5160atcaagtgtt ttactcttaa cagcttcatc tcctagcaat ttccattgta ttttttcatt 5220cataaatgaa cctgtttccc ttggcaatgt tatttggtta gctttggtta tttgagctag 5280ggtcttgccc ggtgtgtact gggctagatc agaagtagct gtgtagttta gtctagagta 5340gatcttgcag cagctctctt gtatctgcat cttgagtctt ggaattacag aaatgggcta 5400ccacgttcta ttttgaccat actttttttt ctttccatat gttccaaatg agcataaaac 5460catcaaactc tggtacattg tactagtccc ataggcaaaa ttatattcaa ggtgagcgag 5520gcagtgatgg caaggtgagg ttaatgtcgg gttgtgatct acaaaaggta gagcctgggg 5580tgcctgggat gtactgaatc aagctttccc actgccccag ctgaccatct gttgtttcct 5640atcgttccac acccaaccac agacacatta tacccctcta ccacacagct tttaaagaag 5700aacgtgagca tcacagacag gaagtgggca atggagaagt ggagaaactc agtgctcaga 5760ggaaaggaag cagtgtggtc tgcaagcctc atgcactgta catcctttga gtcttcttcc 5820actgtcacca tcacaccgca cacagaatga ggacaaagga gagagctcag acactacttg 5880tacacacact aagacctttt taccaaacag tatttgaaag gaaagcactg cagatgccag 5940ctttggtgat aatgtacttg catcctgtga gccactgtta actgaagatg agaaaaaagc 6000ggacgggaag ttgtgagaag gtgcatctca taaggggagc tcactctgca cagataggag 6060ttccttccta ggactgtttc cccttagatt ccataatggt gaaagtgcct tcaccccttc 6120cattcagaag atactggaga ttccggttta tcatcacatc tgttcgttag agactcagac 6180aggcttgtgc cattcaattc ttttataatg gttattaaac aaatcataat ctgtgattat 6240tttcatttct gatgaggaga gagattaata atggatgggt ttagtctctc atgcactgcg 6300cggtcactgg agcctggaaa ggcagatctc gccctgcatt tggtggttgg tgaaggtgat 6360ttgaactgtg gaagcctggc tctagaggtt tcagagaaga attttaatat gtagccataa 6420tattttggtg aagaatgtgg ctgctttttt gcccttgtct gaagagtcta ccggaggcta 6480aggtgaagag atttagatta attcctttgt taaaggaaat ctcaagacat cctgatataa 6540attctgttga gtggttacta aacttctctc ttacaaagac tgttttaatg aaaagaagca 6600agctgagaaa ggaataatat aaaatatatg gttgcagtat taaaaggggc accaggaagt 6660gaaatggagc tgaatcctgt gttcatggat attaaattga attaatgggg tggtgacttt 6720gaggcaagag tccatccagc taaatttagg tccaggcatg gtagtataca agcctttaat 6780cccaggagtc aaggcaagca ggtctctgag tttaaggcca gcctaggaca gagcaagttc 6840taggtgaaga aaatcttaag tgcaggcatg gtggcatata cctttaattt caggagacat 6900ctctgagttc aaaatcaatc tacagaacaa gttccaggac agccaagctt aggaagtgaa 6960ggagttggaa aacaaaaagc tggtaataga atatgggggg agggggggaa gggggccatg 7020ttctaatccc agcatgcagc agaactcagc agctttgacc atgtggctct ggctttagag 7080tccagaatag aagggactac tggaacaatt gatgctcgtt agctggagct aagacattag 7140tgatgattaa gaagagaccg gtatcactga ggtgtaatct ggcgttttct gagagcacaa 7200agaagctgtg ttccagagat agccaatgtt gtccctcgtg ctgcagctgg acttggtagt 7260gtgtaagaac cacccaagtt gtactggttt tgaaggtatg gaggagtcat ggagagcagc 7320cgaggctgct gtgagaggcc atgggaagcc actggtgaag gtgcagcatt agttgtagtt 7380gatggcccag gactgaaggg gccatgcaaa gaagttgagg cttgactcca tgaagagagc 7440ctacgagagg ctattggtga atcctagttg cagtggaaga ccccagggta ttggagatgc 7500cactaccatg gggatgatca ccaagaacag cagcagcagt ggagtagagt caaccagagc 7560ctagagtgct acagagagca gagctggaga tatgacccaa gccctttgaa ggagtccaga 7620agatcatgtg tggatcccag acattggaaa gagaagctgt aatgttgaag tggccttgga 7680taccctaaga tgttcgagat tgcagagctg tggaatacct gtcaaggaaa gctgctaaca 7740gggagtggaa tcacccagga gaaaaaacct tgttgcagtc agtaaagatg aaaagggagt 7800agagatatga agacagcttt gacatagaca tggagatgaa gagtttggat tttgcccagc 7860tggtgtcctg tcttgttttg gggattacag ttaagtgatt ggatgaatgt cagaagagac 7920tttgaccttt ggacttttaa cattgttgag actgctgtag actatgggga ctttggaagt 7980tggagtaagt gtagtttttt attatgctgt atttaggtat ggccctatag actcatatgt 8040ttgaacgagc ctatgggtcc atggagtaga atgtagtggt ttgagtattc ctagctcagg 8100gagtggcact attaggaggt gtggtcttgt tggagtaact ttgtcactgt gggtgtgggc 8160tttaataccc tagtcctagt tgcctagaag ccagtcttct cctagcagct ttcagatgaa 8220tatgtagaac tctcagcttc tcctgcacca tgcctgcctg gatgctgcca tgctcttgcc 8280ttgatcataa tgcattgaaa cttggaacct gtaagccagc ctcatttaaa tgtcatcttt 8340ttaacagttt cctttgttat ggtgtctgtt cgcagcagta aaaccctaag taagacacac 8400gctataaatc actcattaca atgtataatg tacaaaaagc tcatcttgtt gaacctttca 8460tcccaaggca actgctaagg tgtattgtag acttgtccct cctggaggtc ctgggctgtt 8520aggggaccag ctcctttgtg attggcatgt ttccaaagtc cattcacgtt gtatcatgac 8580cagtagttct ttttattgca aagtagaatt ttaccaaatg catgtggcac ttttatccat 8640aattcagttg ctaggctttg ggttgtttcc actttttgcc attcatgggc atggtttaat 8700gttttctgta tgtatctaga acagcaattt ccatattaca tgggaagtgt tgaaatgtat 8760gaaaactgct ctgtaaccct atatcatttt atatccttga aagcagctta tgaaggatct 8820gattcgccac acattttcta atgctctttg ttatcgatgt cttttttttt ttttttttaa 8880agatagggtt tctctgtgta gccctggctg tcctggaact cactctgtag accaggctgg 8940cctcgaactc agagatctgc ctgcctctgc ttcccgagtg ctggtattaa aggcatgtgt 9000caccactgcc cggctttatg tctttttatt atagacatct tggtgggtat gagttggcaa 9060accattgagg ttcggattgt gtttccatag caacttatga tgaggaactt cttttcatgc 9120aatcattgtt catctctgta gcatctttac agatgtgact tcccctttta actaggttat 9180gtatattttc tccttataat ttgttttaaa ctaatttttg tgtgtatgtg tttatgtgta 9240tatgtggtgt gtgttttcct gcctctcttt acccagagct ggcattatgg tttacacagc 9300ttcttattga tctattgttt aatgtgggtg ctggagatcc agttcagatc ctcatatttg 9360catgacaccc ccttaaccaa ctaagccata ttccccatcc gcaagctcat ctttaatgta 9420agatagatgg ttagtaatca taactcagga tgtatggtca taaaatcaat atgcattgag 9480tgcaggtata tgaatcatac aataatgttt attgccattc atcatgcctg ctggatagtc 9540aatcagtctt tcttctctgt cttttcttag gatcccattt gtcagctctc aagccttttt 9600agaatcctgt gaacatttgc caaagttgct tttttttttt ttaaagagag ggttgcggct 9660tcttcctagg aacagagaca tctgcatttg ctctcatgcc taacgccact gaagctggaa 9720aagccactga tcctggacat ggtgagcaca catctgagaa caagtcacca gaagagggtc 9780tacaaggtgc tgtaccatct ttctacacaa gtgcctcaga agcacccata gcgcccagag 9840gagatgggca ttatccatcg agttgtccag tgactcacac tcgagagaaa atttatgcga 9900tctgctcaga ttatgccttc ctcaaccagg caacatcagt ctacaaaact cctagcctaa 9960cccgctctgc ttgcctccct gataacacct ctctttctgc tggaaatact acaagatata 10020ttggaatttc aactagtaca tcagaaataa tctataatga aggaaaataa cttggaaaac 10080ttgtccactg gcatgggcaa gctacctctt gcatgggaga ttgataaatc tgaatttgat 10140ggggtgacta caaatttgat acataagtca ggtaagaagg agctatgaag tttacaggta 10200acaacaatca gaaacgaatg ctatctattg ctaagtcttc caatgaaatg ttttttgttg 10260ctaagccagc agcatcattg ccatcttatc tgtcattgca gtttttggtt ttgttttgtt 10320tctttcgtaa gtaaccctag atatggttag tctctgactg tgttgcccat ggaaacttct 10380aatatcatat gtgcatttga gcagctttga aaatcaaaaa gaacaaaata taagtattaa 10440agataataca gtagcttcaa aaaggctact gacataacta gaatattacc attatcttac 10500agttttgcag agatgtgata atattttcta attcaggagg tattaagaca tttttgtttt 10560gaaaaaattt gagttaaaaa agaacattca ttttgatcaa agtcttgatt ttatttaaag 10620ctacaattat gtggctctct tttctaaacc atattctaaa gtccatttta tttctcatgt 10680tattttaacc cgctctaaga gtctagctct ggacttggat acaatcttga taggaatacc 10740gtttctgatg gttcaaattg ttttaaattc tcttcctgct ttctctctag agaagagtag 10800tattctagaa agcacaggta ttactttgag acatttgcag ataccatttt cagaatgcat 10860ggccagtcct ctaattctgt tgtaacttct ggcacgggtt ttactttatc tgaacatttc 10920ttgtattact tctttgttct gtaggcaatg taaagaaaca attttccaag aagaaaacgt 10980cagataaaaa agggcggcat cagagggagt gtctccatta ttctcctctt gatgatgtta 11040aacaacgcaa agtgttagac cttaggcgat ggtatgtggc catgtcagtt tttacttttt 11100ccaatcttaa aaatatgtaa tttgacatta attttcctga gtataggtta ataaattata 11160ttaactataa acactgttag ttccaaaatt atgtctagat actttaggta tatctccgat 11220tttggaagta gtctagttta gctcagcctg gctgtcagtc tcattcagtg atccctctta 11280atgttaagcc acattggctg acacttaaag tcggaatagt cattcacctg gttgttcttt 11340gtgactaatt ctataggcag tgatggtagc ttacagctat tttaattatt gccataccta 11400gtaaaatgaa caatatttcc tgtatgtata ctttcagact aaattgacac tttcctttct 11460agattgttct aaaagttcat catatgcgtg cttgtttgtc atggcccgtg agtttcaatt 11520tagagtttcc agctttcttt ctttgtctcc gtctctccct ggaggatttg tgttttcctt 11580ttaggtcccc agtgacagtt ctgttctggg gatttctgcc caggggttca tgggatgtgc 11640ttagcctgag gagaatctct gaatctcttg acaaaaggag agcggtttgc tttgcttctc 11700cagtatttca gaagctgcag aggatgcctg gcccactcaa atgagaattc acatagacat 11760ttgagtcgtt gccatcaaaa tttcttggtt tgaataagac atactttagt aggttgctgg 11820ccataatgta gctcatcttg ataaaacatg atttttatgc tcatgcattt tttatgtgtt 11880taataggtag gggatacttt aaacaaaaaa tgagttacac tgcattgaag tttgtattat 11940ggtcacgttt taaatcaccc ataaactata tatttctgtt ctgcatgttt tgtattcatt 12000cgagtgaaga atactgtttg atttcccagg tactgcataa gccgaccaca gtacaagact 12060tcatgtggta tctcctcatt gatttcttgt tggaatttct tatacagcat aatgggagct 12120gggaagtaag tatgccagtt tactgctgac accaaactcc atctttgaaa gtagtgtcaa 12180ggaaactgag tatagtgatg cacaccttta atcccagcac tcgggaggca gagtgaggca 12240gatcactgtg agttggaaat cagactggtc tacagaatag attctaggac atctagggct 12300acatagagac cctgtctaat tttaaggtac ctgtgttata atggaaagtc tctgagagtt 12360gactttgtac aaactgagaa aaattgtgtg tattgttaca agcctttcag gttaaaatat 12420ttgcatgtat tcttttcaat aaaataaatg aaaaatattg ctaaaatgtt tctaagctaa 12480gcctatatat tactaacact ggggcatatt ttatttgcat atgactggaa ctgtgaaatg 12540aaaggaatgt ttcttttata gtctcccacc tattacccaa gaagaggcat tacatatttt 12600gggcttccaa cccccatttg aagatattag gtttggccct ttcactggaa atacaacact 12660catgaggtac ggagctgcca cttagggatt acatacgcct tcctttaatt ctgtgaagtg 12720ataactatgt agtgtttggg gacggtacaa gatagacttt gctgctgggt cgcaggctcc 12780ctaagatatg ccatgtgtgg gctgtgcttg ggtttctgtg caattaggac agtagcctgt 12840gttatgaaac tattgctatg agcaaatctt cttcttaatt tcacatggtt tagacaaatt 12900aatgaccact ttcatgtgaa aggatgctct tatgttctat ataagcccca tgggaagaac 12960aaaacagctg gagaaactgg taggtgaaaa tacacacaaa cacacaaaca cacacacaca 13020cacacacaca cacacacaca cacacacaca caccccaaac tttcaggttg agtacatcgc 13080agaaattggc cacgttgttt cctgggatat caaattaact cttaatagtc tgatgttttc 13140atacctcttt aagaggaaaa tcatgatagt aatatataga gcactttcaa tattcaattc 13200cattttcacc cctttctgat attttctatg taaatgttac aaatttaaag cattgtctat 13260aaacactgtc tcttaacaca ttgttttgtc tgggtttagg acttgttata tttgcggtgc 13320gcatgttgtt tgtgcgtgtg tccaccttct acgcaaaaac cttgaattta cagtttattt 13380taaaacagag tgagagcact tgaggacatg tgatgttgga ctggcatcaa gtgaatacac 13440agaacagcag agtaacaaac tgggaagtga caacactctg ttggttttaa catactcatt 13500aatgaagttg aatttaagga ttatttgtta tctattagtt gaattatttg tattgtttaa 13560tgatttcact tataaatggt cacaataaac tttgaaattt atggagcagg ctgctgtaag 13620gtcctgctgt taaagaagaa acttttcgga ctatcctgga aacgggagac tttatctaag 13680gttgttacag tggggtgttg tgataaggat taaagattaa cctcggcttc aaaggtgata 13740aaggtggctg taaaggcaga tggataattg atgcgaaaga atacagggta ttgagaaact 13800ataggtagta atgcaaattt aacaacattc tcaaagtata aaaatataaa acaattaata 13860aaatgtagta aaacaacaaa agggctcctg agtgttagct taggattagt attcagaaat 13920tttatgattt ttttttttga tcaattgtgc tgtccagtat gttttggagc ataatttgtt 13980agagatttta gtgtatggat ctttagaaca acagtacttg gtttactttc aaatggtcct 14040ggttcgtaca attctccctg agttatttat aaaatgagtg gaaggatatg aatgctgtta 14100tccagtttcc taacggacta ggatatgcta ggctgcttgg caccgacagg cacactctga 14160tttcgtttac ctctgtttaa acatccttca acatatgcac agtcttttac ttatagaatg 14220gaataaagtg ttttccttta caaggtttaa aatgacatat atgcatgtgt ttatttcatg 14280tgtgatcaaa ggacagcctg agggatttgg ttctctcctt tcaccatttg ggccctgggg 14340atcaagcttg ggcaagaggg taccgttact tccaaagcca tcttcctaag cccctccctt 14400tttatctctc tttttatgca aaggcaagat ggtagtttct tcagatttct tcaaatacag 14460tttaatgaca tcttttacca attgtggtct ccccaacccc cttccacctc cacagcctgg 14520caccatttct gaaatgtccc tggacagtct agccccagga tcctattatc tcatgtactt 14580ctatccattg gccccagaat cctattagct catgtactac ttactatcca ttggcctctg 14640tcaggtgggg tttgggatgg actatcatga cttttatctt tttaatgtaa atgtcttgct 14700cagtgcccat agtcaccatc tgaagtagcg ctttgatgct ttgattctgt gtatgataca 14760catcagcgat agttacactc aggtcggcag agcactgata ttctgcttcc tgtcctcatt 14820ttgtccatac tctctcttat agaagagctg ccaagaggag ctttcttcaa tggtttcgga 14880tcttcaatgg ttaaaaccat ggccagctgg tattgcttat ttagtaacta ctcggaaact 14940tccctcccca ccccccaccc ccctttgagg tagatctgat tttcacagtg tgctttcagt 15000gttcttggta cttgcacaac ctggatttag taactcaggt tcttgaatgg cagtgccatg 15060gccctcaata gtcactgtaa atgaagcagc tcatagagtt taaaaaaaca aacaaacctg 15120tgcacatctc aggtgtacag cacactggta ctgttttcct catctccagt gcttttcgag 15180ttcatattga gccgttttct ttaacctgca gtgtggttat gcccctttcc acctcgggag 15240ctctcgaggt ttgcgtacag accaagcttc atagtgttga tagcatgtgg agccaacatc 15300aactggactc atgtgcctgc tgtatttaaa aggtatcaaa tggatagggg ccttgcagga 15360caatgccatg ttgcttgact acttaccata gttccagaat cttcccttga ctttcaatgt 15420ataaagatgt gctctcagtt ctctaaggct cttctgcaga aggactggtt aatactaaac 15480catcttgaag aatgtacggt gctgacagtg tattggagtg agagctataa tgccaaaggt 15540ggccgaaccc attgacagct caagttgtcc actcctttca actctgaacc taattcttat 15600cgctttttcc acatccatcc tgtctgtttg ctgtacagct gtggaggtag tggctttgtg 15660acttggcttt taggtttcct ctgttattgg gtgaactata ctctgaacct cgtttctctt 15720agcaacacat ttgtcttctt atcacagatc atttcctttg gtaactgcct tctgagtaat 15780gctgcattgt cttttatttt agaaggtgat cttttctctg attttgtcct gaaattgtgg 15840aggctttctc tgttttactc tacattctta cctagtgtct tgatgtatgt tcccctgtgg 15900atttgcctct ccttatactc cttcctctgt ctagattgct aaaaccctcg ctcctcatta 15960agttccatct tgaatgccat cccttcttga ttcctctgat ttctataccc atttgtgatg 16020ccttaacatt agctctttca tctttcacgc tagagcttgt cttgcttcca tatggtatct 16080gacagagtta ctaggtactc atgagttaaa tcaaaatctc catttaatct aaactagctg

16140ctttcaaaat actgtccctg gatgcatcac ctgataactt gttaacaatg caaattctta 16200gttcccaacc acagacctat aaggccagcc actcagactg ggctctagta agactttatt 16260gaagtcttaa caacgagatg gtatgtagcc tctttaccag gagtttgatt ttgcattgag 16320agaagatgca gtcattttct aattgtgttc agatgaaacc tgaggattcc ttttaaaggc 16380ctgaaaggga ggtgatagaa gagaactgtg cacacactta ttttagctac agcagctctg 16440ctttaagaat aagtttgctg atcttgaaaa catatgaaga atgttatgta ccttggagaa 16500acaagagcaa acatcccatt actagaaatc atgtgtatag atgttatata tcgtatttaa 16560caatgctaga agcattactt catagaagaa aacccatgtc atggcatttg gaaaactagg 16620acatggtaaa agagtgtttg cagcagacaa aattatgtga ttgccaagga gggcagactt 16680tgttatccat ttcatctgcc tctagaatcc ttacttctcc tcccctatgt gattgccaag 16740gagggcagac tttgttatcc atttcatctg cctctagaat ccttannnnn nnnnnnnnnn 16800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatgatgt gattcaggct 16980agcctgaact tgtctatggc cttacatctc tgttttttta aaatttaatt taatttttaa 17040tttgtttttt ccactccata ttccattcct ctybbyyycc cccaccccca tccaccctct 17100gactgctcca catcccacac ctcctcccca ctccaccctg tctccatgtg ggtactccta 17160ccccccaccc cacctgatct ttaaactccc tggggccgcc agtctcttga ggttaggtgc 17220atcatcttaa aattaattta atttttaatt tgttttttcc actccatatt ccattcctct 17280cgcccccccc ccccatccac cctctgactg ctccacatcc cacacctcct ccccactcca 17340ccctgtctcc atgtgggtac tcctaccccc caccccacct gatctttaaa ctccctgggg 17400cctccagtct cttgagggtt aggtgcatca tttctgaatg tacacagacg gaagtcctct 17460actgtatgtg tgttgggggc cgcatatcag atggtgtgtg ctgtctgttt ggtggtccag 17520tgtttgagag atctcggagg tccacattaa ttgagactgc tggtcctcct acaggatcac 17580ccttctcctc agcttctttt agccttctct aattcaacca cagggtcagc tgcttctgtc 17640cattgattgg gtgcaaatat ctgcatctga ctctttcagc tgcttgttgg atctttcaga 17700gggcagtcat gatagatccc tttttgtgag cgctccatag cctcagtaat agtgtcagac 17760cttgggacct ccccttgagc tggatcccac tttgggcctg tctttggacc ttgtttggat 17820caggctctct ccatttccat ccctgtaatt ctttcagaca ggaacaatta tctgtcagag 17880atgtgactgt ggggtggaac cccatccctc acttgatgtc ctgtcttcct gctggaggtg 17940ggctctatac gttccctctt cctactgtca gccttacatc tctgaatgag ttcaatcttg 18000cctgatccta aaaggtctag cctgattaat acttgggaga aagagtgaga ttctgtcttc 18060aagagtcaat tactggaact tacactgtat tcagtggtag agcttgcctg acataactaa 18120aggctttgag tacaagtatt attgcaaaac agtattttca gttatctgag aaagcttctc 18180attgattaat ttaaatttaa agagatttca ggagtttttc tatggcaatg gtggtaatag 18240tcatctttta caatgttttc atctacagag tacagttaac tgaatccacc atctggaatc 18300tatttacagt catgctcttt tatggaaatt aaagactgtt ctgaatttat tcctaaaaat 18360tctttatcta aactgccaag gtgggggtca gtttttaaga tgtgaaggaa tacaactcaa 18420tcctggggag tagctagtca tgactggtct ttgtgaagta gagattttag tagagaggac 18480tccctgtctg tgctgatcta cctggaagtg aaagcttatg tggaatgcct tgatggaact 18540tcagagtaga gaccagacac attaacttat gcataggcag agctcccacc cccaaaccct 18600tgtgaggcag tcaggttgcc tctgtggagc tgctgcacac actgactagc agatctcaat 18660ggtcatcagc tatttcagtg gtcagaaaat cgatcgctgt gaacatacca tgcaggcttt 18720cagcttgctt tttttagggt tcccagtttt aatggactac ttgttaatga ataatttaag 18780ttcttccagg aatggaagtt aatgaaatct cagttaatgg ctttgagtac tgagtgctga 18840taaaattgtg tctcttacgc taatactttg gaaatcattt gacagctatt acaatgatgg 18900gcaacagatg aggagaaaca gttgtgtaac tccagcccga ctgcagagct gatgcctttg 18960atgtgtgtga tgcaggtgta gaaagacaat tttcttagtg tttctattgc tgtgatgaaa 19020accatgacca aagcaactta gggaggcatt tggctggtgt gtcctgaatc ccagtccatt 19080gagcaaagtc aaagcaggaa ctcgacaggg taggaacctg gaggcaggac ctgatgtaga 19140gaccatagag aaatgctgct tactggcctg ctccacatgg gttgctcaga ctacttttta 19200atggaatcca gaaccagtag cccagagatg gcactgccca caatgggctg agtcctccca 19260catcaattac taattaagga aatgctctac aggctcccct acagccaaat cttatgaaag 19320tattttctaa atcaatgttt cctcctttca aatgactgta gcttgtggga gttgacacaa 19380aaatgagcca atataatgat aatatttgtt atgtggcagg tgtgagtgtt attgtgtggc 19440aggtacactc aagtgcatgc atgtgtgtgt gtgtgtacga gtgtgtgtgt gtatgcatgc 19500acaccattgt tgaggtttgt tgttatttaa ggtttgtgtt tattcatatg cattaataaa 19560ctttaagttg gatgtagatg ttggatgtta tttaaggttt atgtttattc atatgcatta 19620ataaacttta agttggatgt agatccagac attagaatgt ctataattta acctaattta 19680aggttctttt gttaattctt ttgtagactt agtttatatg tgagcgttta tagacacatg 19740tttcacttgg gcatgcttat tcagacgctg gtagtgaggg atgcccacag cagaaaagct 19800aagacggtga ttgtgcaatt actgggtttt acacaatcat tgaatatctt agacaacttg 19860gtatattctt agaaagttct gataagcaag gacatttgtt ttactactga gtttatctaa 19920aactaaaagt aaattaattc atgtttttgc ccacattggt agcgtctcat tcattccctc 19980gatggaactc agaatttcag tgtattccaa attctgtctt ttagtcctta actagtcctc 20040tgcattaaat gtggacatcg ctttgtgttc ctcaggttgt ctggttttgt ctttgtagct 20100ccaggggcct tatcaaagtt gacccgagga ttgaaagatg agtcactggc ttatatctat 20160cattgccaaa atcactattt ctgtccaatt ggctttgaag caacccctgt gaaagccaat 20220aaagcattca ggtaagcatt ccatacttga taaaggcaca gcactaggaa aatttgatat 20280acaaagtaat tttgggaatt aaacaaattc tagtctctta gaaattggga agcatggtaa 20340tgtgttttat tcaaaccaag tgtgcatatg ttttgcagga ctgattgcac ttcagagctg 20400ttgctcagtc attgtaagaa aaagccatct gatgtaccac ttttatgatt agtatacaca 20460gatgcagggg ccttcccatg ttattccctg tgtcctctcc gttgccatac atgtccttgt 20520gtcaagagtg ctacaactaa gtacattaag aagcaccttc tatgctcata gtgaagaact 20580aaggaattgt taggattttt ctccaaggga gctcaggacc agtttcatct ctaatcttct 20640tggttatatt tgcctcttgc ttttcttaat ccagtttctg tctttttgaa caacgtgctt 20700ttacacacac ataaacttgg gtctctttga aggagatgct tatgtgctga gaggttgccg 20760agtttgaatg ttattctctt taccaagttc ctcataaggg acctgaaaac agaggttagg 20820tttaagaaca ggtggcgtga ctgtcttacc ccgctgctct gctggttgat gcacccttgg 20880attgctgaga ttggtgatta gggaggatga aaagattggg aggctcttgg aagttatctc 20940ttaggtgaag gcccagattc tggctgaaga acaggcttgg cctttaggac tggagggttt 21000ttatgtactg gaggtcatgg atccctggag tcccactcaa aggtccttgg atgtggtgcc 21060taggggaacc tggaacagag gagcagaagt tggaggactt aggaggctgg atactacttg 21120tgtagaagga gcaagagtaa cactgctttc tctccaactg ctgatgtgct cggttcactt 21180tctggagtga ggggcacttt tgcacttctt tgatggctcc aacctctgcc tttaccttcc 21240ttcaggtacc tatcaaatgt aaaacatgcg ttgacttcta ctgctctgct cctttaatag 21300ttaggcttcc ccagtgaatg cctgtgccta tgaaccatct gatttttttt cttaaaaatt 21360atgtaaatct ccaggctcct ctccagactt gaattagagt ttctggggat gggaccgaaa 21420cattgtgtgt ttaaaatcat catcatctcc tctgatccaa gggggtgata tgttaaaatg 21480cttataaccc tagattttga atatggaagt aataaagctc aaacctaatt caaggatttc 21540tagaatggga ccaaacctaa gtgctcatat ccatcctaga ttaacaaagc catctttgag 21600tatcagtgta ttttagttca aacagcattc tctgggggag tgaatgccat ttatcaagct 21660gattgtgaac gtattttctt tttagcaggg ggcccctctc ttcacaagaa gtagaatact 21720ggattttaat tggagagtca agtagaaaac accctgccat tcactgtaaa aggtatgttt 21780tagtagcctt tatgttttct ttaattggta atagttccgt caccgaaaga acagatcaaa 21840attgtgtaac tagatgcatg tagataaatg tgcacacttg tgtggtctgt actctgtaca 21900gtcttgccac agaaagttct gcattctact tctctccaat ctcacagcta accggttatg 21960tttcccatta ccacagaagt cattttcttc tctctctctc tctctctctc tctgctttcc 22020catataagta acatattaag aactttttct ctaaaaataa ttagaattgg ttcatttctg 22080ctaaaagtcc tgatgcatac ttgattaaaa taaaatcttc aggactggtg tggtggctta 22140ccaggtacag gtgcttgcat gcagtcccga tgatctgagt tagattccag gagtcaacat 22200ggtagaatga cagaaccaat agttctcttc caaactccag acatacagaa tggcatgtgc 22260atgcatgcgc ctgtctgtcc tccctccctc cctgttagtc tctttctctc ccctactctg 22320tttccccaca cctgctgaga gctcctggcc cagagagggt agagacaggg catagagtag 22380gactctggtg tggctttaaa gactgcagat ctccagacag tctagctctc taactgtact 22440aaaatgctgg agataccttc tgaagtccta caagctttct tgctaattct gagagtaaaa 22500agctgttggc ttaggtgatt gacacctgta gcctgcagtg ggggattagg taaagtggcc 22560ttttcctatc tcagcaggaa ctgcacagct ctaactttca gcctgctagt agaagtccca 22620ggaagaaaaa gggactcttt gaaaagtgtt tatagcattt attactaagt tgtaaaaagt 22680tacccatgaa agctatctag gagaatgggg ggtagcagga agatcctaag tggggcaggg 22740aataattttc cctttggatc aataaacttc tattgaacca ggagacaaga ataatgcttg 22800cagaaggaaa aactttacat aagcaaatat gcataaatgc acatgaattc atatgcagat 22860tcacacatac acatttatac atatccattc aaaccagtta gatccagctt gcattcattc 22920ccacaattac acacacacac acacacacac acacacacac acacacacac acacagatcc 22980atacttacat atgcatttga aacaaaagac caagcaccag tccagaaaga actcacttat 23040tctggatgaa aaatgagctc caatctttat tgcataggga gagaaaaggc ttctaccatt 23100tgaatgccaa atgaattaaa ctcatgtttg taagaagaaa gctttattcc ttttaatagt 23160actaagcttg ccttaagagt agacctgttc tgttgcatgc taagaagcct gcctgcttct 23220tctgctctct cttaagtttt gtttatatct gactaaaaaa tgttttgtct aaaaagattc 23280tcctcagttc agttatgact cttctctttg acctcagctc tgaaacacct ttcaggatac 23340atggctacac gattaaaagt tcaccacaag tttacacaaa ttcaaatcat aactagaata 23400ggaagtttac gacagagaat gtttacatgt atatccatta ggagtaatta tctggctaac 23460catttgccac ctgtcacagc tccacaggtt cactggaagt taaaaactgt aactgtgaag 23520gtttgtatag atttaccctg tcaaaatgtt atcttctctc ctaccaccta taataaattg 23580ttagttccct ttttatgacc gtagttaatg gttgtagaaa gtctggttac tatctagctg 23640gcgacacaat ggaagactgg cagagttctc attgcagttt tgacaatcag aagagaccta 23700atagcacttc cactataaaa gagcttagta aatactgata taattttagg aattcttata 23760gggtcatcat taagaattaa gaagtcattt atttgtctat atagcattac tacaagacag 23820tacatctttg tagatcggca gaaagctact caaaagggtg ggctaatatc tagtgattgt 23880tgtatatgtt taataataac aggagaaata tattaatagc aggaatcttt cctaaaatga 23940ttttcccctg ggccttgcct atgggatcaa atctgtcatg gattacaatc cgaggcagac 24000cgtctcagga agatcacctg ctagttatta gcttgtccta tgatggctcc tgacacacac 24060ccttgctctt tttcactatt gcctgtgcat acacacagac tgacacaatc tgaggattca 24120tggtgtagtt aagggagtac tgacatcata atgacatgga ctcttacaag ctaacatcaa 24180ggactctatc accacatact cagaacttta atttccttta gcactgattt tgatttttca 24240aaaaaaaact cataatgtcc tttaaatgat ttttcctatt gtaaattaat ttaaaaattt 24300cattttatgt tattgctggt aatataaaga aaaagaactc cccgatttta ctatatagac 24360cttgattttc acatctttgc caaatttact tattatttgt ataaaatgtg ctttgatttt 24420tctccacaag cattttatta gtatattgtt tattagtaat tctgacagaa tgtggcacat 24480agtggcacac tggtaaatga agcaaaagga tcctgtaaac caaggggttc aaggccagct 24540tatacaacat acaaaaccat accccattgc tgcccccccc ataagaaaat aaattaacga 24600gtataacaga aagtgtagta tgccactgct ttagcctgcc aggtattatc tggtatgttg 24660acatataaga ataggaccag ttataaactg agaacattga cagtaattgg ataatcataa 24720ctgtcactta cacacaaaaa agaagctttt aaagtctagt tgatggaaca atgctgacct 24780aagagaaaaa tcaccaatta ttgtggcagc ttgctcatgg aaaaagctta ctaaagtggt 24840actttgaacc tgaagaaaac tgtccttttt cttactatag atgggcagat attgtcactg 24900atctaaacac tcaaaatcca gaattcttag atatccgaca tctagagagg gggctgcagt 24960tccggaaaac aaagaaggta agaatactat tacattggaa gtaacttgtc gaatgaccag 25020attgcaatat tttattaata atcttgtctt ggtaaataac agaaaccaag ttcaaactac 25080cttagtcagg aaagtaagat tattttaagg attcaggcta actttaagct tccagctaag 25140gatagtcata aggaaatgac acacagcact gcagtaatgg tagaatggct tatgtaggct 25200gtgctgtact aagtgcttta tctgaatgta aatttcttga gcaataactt tcaacttttt 25260attctcaggt gtagattgag acttaacatg ccttggttga gctccatagt cttggcctaa 25320gttgggtaaa ttatggtcac aagacagagc caggtgttca agcatgacta cagaaagaag 25380ctttcagaaa cagatatagt atagattgag taggcaagct tttgctataa atgaagctgg 25440gagtggtagc acatgcttgt aatctcagct cttaggcgag aggattttca tgctatctta 25500aactattgag attattatct caaaagtctt gtgtaaacat acacacacac acacacacac 25560acacacacac acacacacac acatgcgtgc gtgtgcacgt gggagggaat aagataggat 25620tttagtagaa tggaggagag gagactacac acacagcaaa ggaaaagcat gattttaatt 25680tgtgagtcag gaatatgtgg atatgactat gccgactcta ggctgtggag gggcatatgg 25740gacacagtgc tctggtccct aagggcttta ttataatgta aaggcaggtg ttgggaaagg 25800tgagtatgtt aaagtgttct gataaagcag agtagtagtg ttttcagaca tgtggaagaa 25860taccaccatc acctcaaggg agtcaataag agccctaaaa acttacaaac ttattttctc 25920tttattgtct tcatcttttt tcccttctca ggttggagga aatttgcatt gcatcatagc 25980attccagaga ctcagttggc agagatttgg cttttggaac tttccatttg gaaccattac 26040acaagaatca caacatccca cacatgtccc gggaattgcc aaatctgaga gtgaggacaa 26100tatctctaag aagcagcatg ggcgcctggg caggtccttc agtgcgagtt tccatcagga 26160ctcggcatgg aagaacatgt ctagcatcca cgagaggagg aacagtggct accacagctt 26220tagagattat aatggcaatg actgaccatg ccaaaactta gccactggtg ttacccacac 26280agctgttatg tacaggactg cattaggaca tcagctggtt ttattaagtc tgtcaatagg 26340aacagatttt gtggtacaaa acacaccctg tagttctcta gtaaaaaagc ctacatagga 26400ttactatggt tggcttcaaa tatacaggca ggtaagcaca gaaccccgcc cttctaaagt 26460taaaagtaga taagcaatct ggacaaaggg tttcacaaaa tccaatacaa tcaaaacggc 26520ttcaaagcaa aaacacaaat gcatttaatt tgaaaagcat cgaaacttga actacttaag 26580catgaagcga cttattgata cttgatccct agcatttatt acaacacttt aattcctaag 26640gcatcatctg tccttaaaaa atgggggcag tcaaggtcta gtttttgctc atggttaaaa 26700ctaatttaaa attatctttc tagtctagtt gttctttcag tgctaacagt atccacctcc 26760catcgttgct ttcctgaata actctcagga ttctccaaaa agcagcagaa actactccag 26820aaactgacct tttctctagg tgcagatagg tgacttaggt cattgatcct gatactcttg 26880acttggcacg tggttgtgaa atagctacaa gaagaatata ggtctggagc gaagtctgat 26940gttctagaac aaaccttgtt tcagggatat agttagagag cacttggcat ccaaagtttc 27000cttatccacg gtaacatgtg ctgtgagatg tcacatttga cttgtctctt aatggagtca 27060tgtgttaaca acagcactga tgtcatgttg gcaatgtcca gctcactctg aggaagactt 27120tgtattttca actctgagcc gtttcctttt gtgaaacctc caagcaatta ggtgttggaa 27180gtgtgagtta catattctgg aagtgtgagt tcaatacttg agctcctctt tagcggctct 27240tgttttcctt ttgctgccaa ggtgtgactc atagccgtct atgatgctgc tctttcacgt 27300cgtaggttta ttccaggatt caaatcagta acttggtgat tacaaggtgc tgagtatgtt 27360ggaaccattg caatacacct caaagggagg tgtcggattt tgacttttta aaaaaaattt 27420tcatttttct cttgaatttc atatccatct atccactcat atatgtttag cctacagaat 27480tacaaactag tcctgtttct gaagaggttc tttagcttga aatgtaaagg actgaaagat 27540ttgtaggtgt tgcttttgtt acttcacact ggaactttga aaatgttttc atcaaataaa 27600gttttgtttt ctacttttaa tcctatgaat tttaatgtct atgtttaagt tagcgtgtat 27660tcttgtaact gtgtgaagca gatgataatt tgctaattcc atgtaatcag tgttataaga 27720aacatcttac aatttttata atcaccggaa caatgtgaaa agccaataac ttccattcca 27780tgcttgctac ttttcaagta cttgagcact gattctctaa tccttcacaa cgtgatttag 27840gaattttctt atattaatta aaaaaaaaag gttggaattt ttgtggttca ggcatatttt 27900caatataaac tgcttttaat agttcaattg agtatttcaa agcaataggt ttgaagagct 27960aagaggaaag aatacaaatg caaaataaga tgtaaacctt aagaacaaag tgacagctgg 28020gaaaataaga tgagtttatt tcctgtacac taatcatatg ctttattgaa atcactgaca 28080gacatctgac cacttaaaac ttaagcttac agatttaaag atgacttaga gcacagaatg 28140tttgaattca gtgggagttt tttttttttt tttttttttt tttttcaggg tatgtaggta 28200agaggtcctg caaagtccct tttgaaaact agtaatattc ctatttgatt tgttagtcaa 28260cctgtcttag aaattgtcat agatcttcta aggaaggata ctgattggct cagaagttac 28320aagttgtctc acctgtgaga ttatgtcctc tttggtagtg atgtggaaga acggttacgc 28380taatgagcat ggtatttata atagtagttg aaaagctatt taaatgcttg ctataattat 28440tattttgaaa atgtgttctt aattgattag tctgttatca gcatggcaac ctgcaggcag 28500gcatggcact ggagaaggag ctgagagtcc tacatcctga tcggaaggca gccaggagag 28560gacagtcttc cacaggcatc caggaagaag gttcttttcc acacgaggag gagcttgaga 28620ataggacctc aaagctcacc cctggtgatg aacttcctct gacaagggca cacctcctaa 28680gagtaccact tcccatgggc caagtatttt gaaaccacca catttataga acctgttaat 28740cctttatgca ctactacagt tacaggggta tatcccatca tagaaagcag gtatagcaac 28800tgaagtgttt ctcaagttgc tttgttacag ccatggagtg agcctaggca accactccag 28860aacctatggc attatttaac tcagtttcac tcttagcatt tctaccctgc caatctttca 28920cttaaaaaaa aaaataaaga caaaacaaaa gccctgatct ctatgccatc actttctagg 28980tgctttcata ggaaaatcag aacagcaccc cagctgttgg tggtagtgac gtgtgccagc 29040taaaactgag gtttgcattg gtggagcatc ttgcctacgt ttatagagat tcttagtttg 29100ctagagcttt cataataaag tactcaggtt gcttaaaaac aagtttgttt ttgtttcgtt 29160ttttttttcc ctcacagtta catagaccaa gacaaggtct ggcacatttg gtttcttatc 29220ttttggactt gaagctggtc accctttttg tcctcccatg gcctttcctg tatttgtggg 29280catactttat gatgttccca ctccttctta ttagtataac cgttatactg gattagaggc 29340caccctgata gtctctctgt atatcaatgg tgaggtagaa atcacatcca tggacttaga 29400cgtatcttca tgtttgtgat ctattactga ccatgactct gtcagacaca gaaatggttt 29460tgttaagttc tggttcatgt gcccttaggt gcctaatggc cttattgttt tgacaatcat 29520tttctaaaaa tcactttgaa tacaggcaca ttgtgcagta cttatattca acacgtgaat 29580tttgaattct acatggatac tgacctgaat acatagtaat tccgcggtcc agccaagatc 29640tgaactatca tctgcatgac ctctactcca aatattttcc acaggatgca aaatgtcctg 29700gggagcatca gacctgagac ttgagaggtc acttaagcaa cattgacgaa ctcccgctat 29760gcactactaa ggaaggggtg taacactcat caagattgat accagtttca acatgtttac 29820agtctactgc tggaagtgat aaaaattaaa caggcaatta aattgccttc agtaaaatac 29880gactttacta aaatgcaaaa gtccgtaatt ttacacattg agggaatgct aaactgtaaa 29940ccacctgttt ggacatagat acgtttttgc taaaatttga cttctgatag acttcagctg 30000taaatgaatt tttttcccca gaaattatct acttctatcc cttccttttt tttttttttc 30060ttagacaagg ccatgtgagt cttgagcttg tgatattcct gcccatctcc cagatatttg 30120tgccaccatg tcgggctatc tattgtttct tatcaatctt tttactgggt ttgaataatt 30180acaataccgt ccatcaaaat tgagccattg tatgctaagt gctttgccca tattcactct 30240gctcggtaaa agattgttct tttgtactgc ttacagatga cggagaagaa attctgaaga 30300aacgtaatct ccacagagct aactctatag taaggcctca aagggctgca ttcgatttag 30360aatgtgcata tcgtgtcttt tcctggctct actctgcccc aagtcatttt tcccaattgt 30420ggaactcaaa gtttcagatg ctggaggaaa ttcaaagtta agcactgcgt tgtactgcca 30480tccagttact gcacgttgtg aaaatatttg ccttcaccaa agtcaagatg ctcatctttc 30540cgttcttttc ttgtcgaacc caatacttgg cgccaaatcg gcaaagctgg ggaggcaatg 30600gggagactga tactgaaaat gcacaaagga gacctcactc cattaagaaa gccaatatgt 30660gcaaacactt tttgcgccta acagtcagcc gaacagaggc acagattcaa aaccgaatcg 30720ccacacacac tgacttcggg agtgctcggg cgtgggattt acgtgtcaag tgtcaacgcg 30780ggaatcccaa ccctcgcgtt cacttggaag ccaagaggtt tcatcgtttg gtcagcggga 30840gaaatcactc gcgaccggag gaaggctcgg cgctggcgtc accggtgctg cttctcagta 30900cttttaggcg tgccagtact ggggctgaaa cctttctgct cctcccacca catttaaatt 30960ccgctgttct ttcgggagac cgggtccgct ccggaagtgc gtcagcgccg tgttcctcct 31020tcaagccctg gtgacagcgg gttccaggcg gagagcggtg ggctctgcgt gagttggtgg 31080cgctgcgtgc tggtgcgggg cgcgtccttt atcctaacgg gaaacaaagg ccccgcgagc 31140ccggccgaga gagagcgcct gcgccatggg agtgcagggg ctgtggaagc tgctggagtg

31200ctcgggccac cgggtcagcc cggaggcgct ggagggcaag gtgctggccg tgggtatcct 31260tcaaggcggc tccggagcgc cggatgagcg cctgctcggc gactgggctt cgtccgggac 31320ttggtgctgg gggtcgattg ggtgtctgtc tatctagagt gcaggcctgc gctccttagg 31380ccggcccatt ttgtgccccc tatgctttag ttctcacagc agagtggcta cagagttgtc 31440gcggagaata aggagattta aatgataaaa tttggtatag cgtcaggctc cgtagtactg 31500tacatctgag aagtcattct gtcagagtag agggggggaa aaaaaagaag agttccgaac 31560cctcccaccc tcctttttga atagttacct cttgtttgtt ggaataaaaa aagttcatac 31620ggtgtatagt gaattagttt cctccagctt ctgtgatggg tgtgtgcatt ttagttttgt 31680gtcattggag ttttgttttt ctcccacttt gaaaatgtga taccaatttt gaagttttgt 31740ttacgtttat ctgttccacg gcccgtttca cagtgcctct tttatcttag aaggtacttt 31800tacatagttt tcttaatgaa aactagaaat tcctgtaaat agttgctgaa tgtgcgtaca 31860tgcctggacg tgtgtccgta ggtggtggca tgccatgcca ttctgtaaac tatgtcagtt 31920cttcacgatg tgcccagctt ataattaatg gagaacttgc agttttgaaa aatcacattt 31980tatgccaaat acatcaatga caaaaattaa aaatttgtct tacaagtctt tgcacagggt 32040accataaacc ctagtggttt ctcggcgtca ctgctgttta aggatttctt cattatttat 32100gatggaagaa caatttttag tgagggggtt cttaaattct catcaaaggg ttttagtagt 32160ggtagagcac tttcttagta tgtatgataa accagagttc cattcacatc tttgtgagaa 32220agtattgctg acaacaccat caaggagtta cgttattttc tcttccagcc cttataaaca 32280attaggaagt taagcatcct agcattcagg gttgtcaaat gaaaagtaat ccatgtagat 32340tattcataaa tggctgtgtt ccatttgggg tgtgaagtta tgtttatgag gttattaagc 32400tcttctgaaa taatggatcg tttaaagaaa atcgtgaggg attttgccta cactcacatg 32460taatctggtt acttctgtaa tttaattttc tgtgtttcat catgagaatt ctgatatctg 32520aaatttaggt gttagaaatt tattcggttt gctaataaat aaatcatcca caattatctc 32580aggtttgtaa tttacatact tttccaaata aactgtttgg gttttagacc atgtgaatag 32640ctgagtgtat tatttaaaca gtttatgtct gtgtcaaaaa aatgttagga aataacagat 32700tagtttttcc tcattattga ccttaacatc tgttagtgca ttggcagtat taagcagata 32760gtaccctcac tggacacatg aacaacatgg attaacttag tctccagtca cctaattcca 32820ggactaagga aaattcccat ttattttcag atgttattgg ttctttgagt ttaaattcta 32880aagcaagttt tgactttgcc tttgcttcat tctttaattc ttctaaccct tgaggagttt 32940agttttgcca catttgtgtt gaagcttgac taatcaaact gtgtaaattc tttcagccta 33000tctgatttta tctgtttttt tttttgtttt ttgttttttg ttttttttta actaagagtt 33060tcatgttgga actctcggcc ttccatgaga agtggtaagc tgtggttaat ttaaatgtga 33120aaaggaatta tttcagtgtg atttagtgtt ttaggaacat ggggtttaat cttttctcag 33180aagctgtagg ccctagactc atatatatga gttaagaggt atcttaactc tgaaattgag 33240ttttctcaat tctaaagtca aaagtgattg attgtataga cacttttgat ggcagtgtag 33300caagcagaaa tggaccaagt atgagtagct gtccagagta gaactgagac tgcagaaggc 33360ttgctctaag ctggatcctg gacctgatca ctcatgtctg agacctacca tcactatcat 33420ctcctttcat gtgggctgct ggaacattct tttttttttt tttttgttat atggattttc 33480atacttcttc tttcttgtat tagatatttt ctttatatac atttcaaatg ctatcctgaa 33540agttccctat accctcccct actgctgtaa cattcttaaa actactctcc ttgcagttga 33600tttttacatg tctcctaagg cccgaagtca ttctggaaac tgctcagatg tgtatattgt 33660atatcagctt ccatacccaa gtgccccacc cctgcgccca ccacagggaa ctgtgtagac 33720gtggctctgc cctaggcgtt tgtatttgct gcgtctcgtc tcccttctgt aactagagtg 33780ctaaactcat aactactctc ctgacctatt atttcttggc acttaatgtg tcaattcttt 33840taggatatgt gcatctcaga cataggggtg catttgtctt gctcacaaat acaatgttta 33900atgtatctcc gtagggtctt ggatttactt ttacatggga gctctttaac atatagggat 33960ggagatacac acacacacac acacacacac acacacacac acacacacac acatattgtc 34020aacttggcat ggcatgagag tcatcaagag tcaattgaga aaattccttt ggaagaccaa 34080gcaagactat agggcttttc ctaattagcc attaatggga gagggcccag ccaattgtgg 34140atggtgccac ccctgggctt gtgtcctggg ttctataagc aggcaagctg agtaagccat 34200gaggagcaag cagctaagca gctcctgtac ccaagttcct gctctcttgg agtcgctgcc 34260ttgattttcc tcagtgatgg actgtgatgt ggatgtgtaa gccaaataaa ctccccagtt 34320tgcttttggt tatggtgttt tatcatagca agtagaaatc ctaagatatt ggcttaaaac 34380acaaaataca ctagcaactt ttgcagtagt aaatgaataa ctgtacatta atttttattt 34440atttattttc ccttaatttt tttattattt aaatgcattt tatacatcaa ccatattaat 34500aatattgagt atttttataa tacataaaaa tgttcaactt ttatattcat atcctttcag 34560ac 345622915DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon A1 splice donor sequence 29cggccccagg gtaac 153015DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon A2 splice donor sequence 30tgtgatccag gtccg 153115DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon A3 splice donor sequence 31caggccagag gtacc 153215DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon B splice acceptor sequence 32ttccctaaag gaatc 153315DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon B splice donor sequence 33tttctgtcag gtgat 153415DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 1 splice acceptor sequence 34ttcctcttag gagct 153515DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 1 splice donor sequence 35cacaaatcag gtaag 153615DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 2 splice acceptor sequence 36tgtattctag gcaat 153715DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 2 splice donor sequence 37tcagacgatg gtgat 153815DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 3 splice acceptor sequence 38gtgttctcag gtact 153915DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 3 splice donor sequence 39gagctggaaa gtaag 154015DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 4 splice acceptor sequence 40tcttttgtag ccttc 154115DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 4 splice donor sequence 41cacttatgag gtatg 154215DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 5 splice acceptor sequence 42ttactttcag gtggt 154315DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 5 splice donor sequence 43ggagaaactg gtagg 154415DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 6 splice acceptor sequence 44tttttaatag cttca 154515DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 6 splice donor sequence 45aagcattcag gtaag 154615DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 7 splice acceptor sequence 46ttatatttag caggg 154715DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 7 splice donor sequence 47actgtaaaaa gtatg 154815DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 8 splice acceptor sequence 48ttaactatag atggg 154915DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 8 splice donor sequence 49aacaaagaag gtaag 155015DNAHomo sapiensmisc_feature(1)..(15)BIVM Exon 9 splice acceptor sequence 50ttcttctcag gttgg 155130DNAArtificial SequenceHSMAP5 primer 51ccatgcctct ctactactca ctcccaacac 305230DNAArtificial SequenceHSMAP6 primer 52ggtaagaaga acaccattgt gtttgaaggc 305330DNAArtificial SequencezfbivmMAPF1 primer 53caatgcctaa cactgtggaa agtgaaggcg 305429DNAArtificial SequencezfbivmMAPR1 primer 54gataactgtc gagctcggtt gagcagggc 29558PRTArtificial SequenceM1 amino acid motif 55Gly Xaa Xaa Xaa Xaa Xaa Xaa Cys1 5564PRTArtificial SequenceM2 amino acid motif 56Trp Xaa Arg Xaa1573PRTArtificial SequenceM3a amino acid motif 57Tyr Phe Cys1583PRTArtificial SequenceM3b amino acid motif 58Tyr His Cys1595PRTArtificial SequenceBIVM N-terminus region of homology 59Arg Lys Xaa Leu Asp1 5606PRTArtificial SequenceBIVM C-terminus region of homology 60Gly Gly Asn Leu His Cys1 5619PRTArtificial SequenceBIVM amino acid motif 1 61Gly Asn Thr Thr Leu Met Trp Arg Phe1 5629PRTArtificial SequenceBIVM amino acid motif 2 62Tyr Phe Cys Pro Ile Gly Phe Glu Ala1 5639PRTArtificial SequenceBIVM amino acid motif 3 63Trp Phe Arg Gln Ile Asn Asp His Phe1 56410PRTArtificial SequenceBIVM amino acid motif 4 64Tyr Arg His Gln Asn His Tyr Phe Cys Pro1 5 10655PRTArtificial SequenceBIVM N-terminus region of homology 65Arg Lys Xaa Leu Asp1 5

Patent applications by Donna D. Eason, Bradenton, FL US

Patent applications by Gary W. Litman, Gulfport, FL US

Patent applications by Jeffrey A. Yoder, St. Petersburg, FL US

Patent applications by Noel A. Hawke, Durham, NC US

Patent applications by University of South Florida

Patent applications in class Binds antigen or epitope whose amino acid sequence is disclosed in whole or in part (e.g., binds specifically-identified amino acid sequence, etc.)

Patent applications in all subclasses Binds antigen or epitope whose amino acid sequence is disclosed in whole or in part (e.g., binds specifically-identified amino acid sequence, etc.)

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Patent application title: BIVM (BASIC, IMMUNOGLOBULIN-LIKE VARIABLE MOTIF-CONTAINING) GENE, TRANSCRIPTIONAL PRODUCTS, AND USES THEREOF

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