Patent application title: Avian transgenesis using an ovalbumin nucleotide sequence
Inventors:
Robert Ivarie (Watkinsville, GA, US)
Arthur Karnuah (Athens, GA, US)
Markley C. Leavitt (Watkinsville, GA, US)
Markley C. Leavitt (Watkinsville, GA, US)
Jeff Rapp (Athens, GA, US)
IPC8 Class: AC12P2100FI
USPC Class:
800 4
Class name: Multicellular living organisms and unmodified parts thereof and related processes method of using a transgenic nonhuman animal to manufacture a protein which is then to be isolated or extracted
Publication date: 2009-08-20
Patent application number: 20090210951
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Patent application title: Avian transgenesis using an ovalbumin nucleotide sequence
Inventors:
Markley C. Leavitt
Robert Ivarie
Arthur Karnuah
Jeff Rapp
Agents:
Synageva BioPharma Corp.
Assignees:
Origin: ATHENS, GA US
IPC8 Class: AC12P2100FI
USPC Class:
800 4
Abstract:
The invention includes delivering to an avian a nucleic acid molecule
which includes nucleotide sequence of an ovalbumin gene expression
controlling region and a heterologous coding sequence operably linked to
the gene expression controlling region wherein the heterologous coding
sequence is expressed in a cell of the avian.Claims:
1. A method comprising:delivering to an avian cell a nucleic acid molecule
comprising a nucleotide sequence about 95% identical to the sequence from
about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1
operably linked to a heterologous coding sequence.
2. The method of claim 1 wherein the cell is an oviduct cell.
3. The method of claim 1 wherein the cell is a tubular gland cell.
4. The method of claim 1 wherein the nucleic acid molecule comprises a vector.
5. The method of claim 1 wherein the cell is a cell in culture.
6. The method of claim 1 wherein the cell is an in vivo cell of an avian.
7. The method of claim 1 wherein the nucleic acid molecule comprises an internal ribosome entry site (IRES).
8. The method of claim 1 wherein the nucleic acid integrates into the genome of the avian cell.
9. The method of claim 1 wherein the avian cell is selected from the group consisting of a chicken cell and a quail cell.
10. The method of claim 1 wherein the avian cell secretes a protein encoded by the heterologous coding sequence.
11. The method of claim 10 wherein the protein is isolated.
12. The method of claim 1 wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.
13. The method of claim 1 wherein the nucleotide sequence is identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.
14. A method comprising delivering to an avian a nucleic acid molecule comprising a nucleotide sequence 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1 wherein the nucleic acid comprises a heterologous coding sequence operably linked to the nucleotide sequence and the heterologous coding sequence is expressed in a cell of the avian.
15. The method of claim 14 wherein the cell secretes a protein encoded by the heterologous coding sequence which is expressed in the cell.
16. The method of claim 15 wherein the protein is isolated.
17. The method of claim 14 wherein the cell is the cell of an avian selected from the group consisting of a chicken and a quail.
18. The method of claim 14 wherein the cell is an oviduct cell.
19. The method of claim 14 wherein the cell is a tubular gland cell.
20. The method of claim 19 wherein the tubular cell secretes a protein encoded by the heterologous coding sequence.
21. The method of claim 20 wherein the protein is secreted in egg white.
22. The method of claim 21 wherein the protein is isolated.
23. The method of claim 22 wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.
24. The method of claim 22 wherein the nucleotide sequence is identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.
25. A method comprising introducing into an avian embryo cell a nucleic acid molecule comprising a nucleotide sequence 95% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.
26. The method of claim 25 wherein the nucleotide sequence is about 99% identical to the sequence from about nucleotide 96,000 to about nucleotide 145,500 of SEQ ID NO: 1.
Description:
[0001]This application is a Divisional of U.S. patent application Ser. No.
10/733,042, filed Dec. 11, 2003, the disclosure of which is incorporated
in its entirety herein by reference, which claims the benefit of U.S.
provisional patent applications, Ser. Nos. 60/462,953, filed Apr. 15,
2003; 60/465,015, filed Apr. 24, 2003; and 60/469,488 filed May 9, 2003,
all of which are hereby incorporated by reference herein in their
entireties.
FIELD OF THE INVENTION
[0002]The present invention relates generally to an isolated nucleic acid molecule comprising an avian ovalbumin transcriptional regulatory control region and linked matrix attachment regions. The invention further relates to recombinant nucleic acids and expression vectors, genetically transformed cells and transgenic avians that comprise an avian ovalbumin transcriptional regulatory region operably linked to a heterologous polypeptide-encoding nucleic acid insert. The present invention also relates to the expression and production of the polypeptide-encoding nucleic acid molecule under the control of the isolated avian ovalbumin transcriptional regulatory region.
BACKGROUND
[0003]Transgenic technology to convert animals into "bioreactor" for the production of specific proteins or other substances of pharmaceutical interest (Gordon et al., 1987, Biotechnology 5: 1183-1187; Wilmut et al., 1990, Theriogenology 33: 113-123) offers significant advantages over more conventional methods of protein production by gene expression.
[0004]Recombinant nucleic acid molecules have been engineered so that an expressed heterologous protein may be joined to a protein or peptide that allows secretion of the transgenic expression product into milk or urine, from which the protein may then be recovered. These procedures may require lactating animals, with the attendant costs of maintaining individual animals or herds of large species, such as cows, sheep, or goats.
[0005]Historically, transgenic animals have been produced almost exclusively by microinjection of the fertilized egg. The pronuclei of fertilized eggs are microinjected in vitro with foreign, i.e., xenogeneic or allogeneic, heterologous DNA or hybrid DNA molecules. The microinjected fertilized eggs are then transferred to the genital tract of a pseudopregnant female (e.g., Krimpenfort et al., U.S. Pat. No. 5,175,384).
[0006]One system that holds potential is the avian reproductive system. The production of an avian egg begins with formation of a large yolk in the ovary of the hen. The unfertilized oocyte or ovum is positioned on top of the yolk sac. After ovulation, the ovum passes into the infundibulum of the oviduct where it is fertilized if sperm are present, and then moves into the magnum of the oviduct, which is lined with tubular gland cells. These cells secrete the egg-white proteins, including ovalbumin, lysozyme, ovomucoid, conalbumin and ovomucin, into the lumen of the magnum where they are deposited onto the avian embryo and yolk.
[0007]The hen oviduct offers outstanding potential as a protein bioreactor because of the high levels of protein production, the promise of proper folding and post-translation modification of the target protein, the ease of product recovery, and the shorter developmental period of chickens compared to other potential animal species. The chicken ovalbumin gene is highly expressed in the tubular glands of the mature hen oviduct and is therefore a suitable candidate for an efficient promoter for heterologous protein production in transgenic birds. Efforts have been made to create transgenic chickens expressing heterologous proteins in the oviduct by means of microinjection of DNA (PCT Publication WO 97/47739).
[0008]Gene expression must be considered not only from the perspective of cis-regulatory elements associated with a gene, and their interactions with trans-acting elements, but also with regard to the genetic environment in which they are located. Chromosomal positioning effects result in variations in levels of transgene expression associated with different locations of the transgene within the recipient genome. An important factor governing the level of transgene expression is the chromatin structure around a transgene, and how it cooperates with the cis-regulatory elements. While the deletion of a cis-regulatory element from a transgenic lysozyme locus can be sufficient to reduce or eliminate positional independence of the level of gene expression, there is also evidence that positional independence conferred on a transgene requires the cotransfer of many kilobases of DNA other than just the protein encoding region and the immediate cis-transcriptional regulatory elements.
[0009]Scattered throughout the chicken genome, including the chicken ovalbumin locus, are short sequences that resemble features of Long Terminal Repeats (LTRs) of retrovirus. The function of these elements is unclear but most likely may help define the DNAse hypersensitive (DHS) regions of a gene locus (Stein et al., 1983, Proc. Natl. Acad. Sci. U.S.A. 80: 6485-6489). Thus, flanking various avian genes are matrix attachment regions (5' and 3' MARs), alternatively referred to as "scaffold attachment regions" or SARs. The outer boundaries of the chicken lysozyme locus, for example, have been defined by the MARs (Phi-Van et al., 1988, E.M.B.O.J. 7: 655-664; Phi-Van & Stratling., 1996, Biochem. 35: 10735-10742). Deletion of a 1.32 kb or a 1.45 kb region, each comprising half of a 5' MAR, reduces positional variation in the level of transgene expression (Phi-Van & Stratling, supra).
[0010]The 5' matrix attachment region (5' MAR), located about -11.7 kb upstream of the chicken lysozyme transcription start site, can increase the level of gene expression by limiting the chromosomal positional effects exerted against a transgene (Phi-Van et al., 1988, supra). At least one other MAR is located 3' downstream of the protein encoding region. Although MAR nucleic acid sequences are conserved, little cross-hybridization is seen, indicating significant overall sequence variation. However, MARs of different species can interact with the nucleomatrices of heterologous species, to the extent, for example, that the chicken lysozyme MAR can associate with the plant tobacco nucleomatrix as well as that of the chicken oviduct cells (Mlynarona et al., 1994, Cell 6: 417-426; von Kries et al., 1990, Nucleic Acids Res. 18: 3881-3885). The lysozyme promoter region of chicken is also active when transfected into mouse fibroblast cells and linked to a reporter gene such as the bacterial chloramphenicol acetyltransferase gene. In each case, the presence of a 5' MAR element increased positional independency of the level of transcription (Stief et al., 1989, Nature 341: 343-345; Sippel et al., pgs. 257-265 in Houdeline L. M. (ed), "Transgenic Animals: Generation and Use").
[0011]The ability to direct the insertion of a transgene into a site in the genome of an animal where the positional effect is limited offers predictability of results during the development of a desired transgenic animal, and increased yields of the expressed product. Sippel and Steif disclose, in U.S. Pat. No. 5,731,178, methods to increase the expression of genes introduced into eukaryotic cells by flanking a transcription unit with scaffold attachment elements, in particular the 5' MAR isolated from the chicken lysozyme gene. The transcription unit disclosed by Sippel and Steif was an artificial construct that combined only the -6.1 kb enhancer element and the proximal promoter element (base position -579 to +15) from the lysozyme gene. Other promoter associated elements were not included.
[0012]Although individual cis-transcriptional regulatory elements associated with the chicken ovalbumin gene have been isolated and sequenced, together with short regions of flanking DNA, the entire nucleic acid sequence comprising the 5' upstream region of the ovalbumin gene has not been determined and has not been employed as a functional promoter to allow expression of a heterologous transgene.
[0013]What are still needed, however, are efficient transcription promoters that allow expression of transgenes in avian cells but with reduced positional variation.
[0014]What is also still needed is a gene expression promoter cassette that will allow expression of a transgene in the oviduct cells of an avian and efficient gene expression regardless of the chromosomal location of the expression system.
SUMMARY OF THE INVENTION
[0015]Briefly described, the present invention relates to novel isolated and recombinant nucleic acid molecules that comprise an avian ovalbumin transcriptional regulatory region and at least one matrix attachment region element.
[0016]The isolated and recombinant nucleic acid molecules of the present invention, because of the presence of at least one matrix attachment region, are useful for reducing chromosomal positional effects on a transgene operably linked to the ovalbumin transcriptional regulatory region and transfected into a recipient cell. Isolating an approximately 195 kb region of the chicken genome that includes regions upstream of the ovalbumin locus ensures that cis-elements are also included that will allow gene expression in a tissue-specific manner. The ovalbumin promoter region of the present invention, therefore, will allow expression of an operably linked heterologous nucleic acid insert by a transfected avian cell such as, for example, a somatic cell.
[0017]The present invention provides a novel isolated nucleic acid molecule of approximately 195 kb of the chicken genome, and truncated variants thereof, comprising a region of about 135 kb that is 5' upstream, and an approximately 45 kb region that is 3' downstream, of the ovalbumin-encoding region of the gene locus. The novel isolated chicken nucleic acid sequence includes matrix attachment regions both 5' and 3' of the ovalbumin gene and an ovalbumin transcriptional regulatory region that includes CR1 repeat elements, a proximal ovalbumin promoter. Interspersed among the elements are stretches of nucleic acid that serve at least to organize the elements in an ordered array. The novel isolated chicken genomic region also includes the ovalbumin-encoding region with a plurality of introns dispersed therein.
[0018]The present invention further provides recombinant nucleic acid molecules for operably linking an avian ovalbumin transcriptional regulatory region to a heterologous nucleic acid molecule insert encoding a polypeptide to be expressed by a transfected or transgenic cell. The heterologous nucleic acid molecule may be placed in frame with a signal peptide sequence. Translation initiation may start with the signal peptide and continue through the nucleic acid molecule to produce an expressed polypeptide having the desired amino acid sequence.
[0019]The sequence of the expressed heterologous nucleic acid insert may be optimized for codon usage by a host cell using approaches well known in the art. For example, codon usage may be optimized for an avian such as a chicken. This could be determined from the codon usage of at least one, and preferably more than one, protein expressed in a chicken cell. For example, the codon usage may be determined from the nucleic acid sequences encoding the proteins ovalbumin, lysozyme, ovomucin and ovotransferrin of chicken.
[0020]The recombinant nucleic acid molecules of the present invention may further comprise a polyadenylation signal sequence that allows transcription directed by an ovalbumin transcriptional regulatory region to extend beyond the heterologous nucleic acid encoding a desired heterologous polypeptide and to comprise a 3' untranslated region and a polyadenylated tail. Any suitable functional polyadenylation signal sequence may be linked to the 3' end of the heterologous nucleic acid insert, including the SV40 polyadenylation signal sequence, bovine growth hormone adenylation sequence or the like.
[0021]The recombinant nucleic acid molecules of the present invention may also comprise a chicken ovalbumin 3' domain. The 3' domain can include a 3' untranslated region of the ovalbumin gene, a polyadenylation signal and at least one MAR that, in combined action with an MAR upstream of the ovalbumin transcriptional regulatory region, may reduce positional variation in gene expression in transgenic avians.
[0022]Yet another aspect of the present invention is expression vectors suitable for delivery to a recipient cell, preferably an avian cell. The expression vectors provided by the present invention may comprise an avian ovalbumin transcriptional regulatory region that can be operably linked to a nucleic acid insert encoding a polypeptide, and optionally a polyadenylation signal sequence. The expression vectors of the present invention further comprise at least one MAR element, and preferably two MARs that flank the ovalbumin transcriptional regulatory region and which can non-randomly direct the insertion of the expression vector into the genome of a recipient eukaryotic cell. The expression vector may further comprise a bacterial plasmid sequence, a viral nucleic acid sequence, or fragments or variants thereof that may allow for replication of the vector in a suitable host.
[0023]Another aspect of the present invention is methods of expressing a heterologous polypeptide in a eukaryotic cell by transfecting the cell with a recombinant nucleic molecule comprising an avian ovalbumin transcriptional regulatory region operably linked to a nucleic acid insert encoding a polypeptide desired to be expressed and, optionally, a polyadenylation signal sequence, and culturing the transfected cell under conditions suitable for expression of the heterologous polypeptide under the control of the avian ovalbumin transcriptional regulatory region.
[0024]Also within the scope of the present invention are recombinant cells, tissues and animals containing non-naturally occurring recombinant nucleic acid molecules according to the present invention as described above. In one embodiment of the present invention, the transformed cell is a chicken oviduct cell and the nucleic acid insert comprises the chicken ovalbumin transcriptional regulatory region, a nucleic acid insert encoding a human interferon α2b that is codon optimized for expression in an avian cell, and an SV40 polyadenylation sequence. In another embodiment of the present invention, the nucleic acid insert encodes the heavy and light chains of an antibody.
[0025]Additional objects and aspects of the present invention will become more apparent upon review of the detailed description set forth below when taken in conjunction with the accompanying figures, which are briefly described as follows.
BRIEF DESCRIPTION OF THE FIGURES
[0026]FIG. 1-1 to 1-75 illustrates the nucleic acid sequence SEQ ID NO: 1 of a region of the chicken genome that includes a chicken ovalbumin transcriptional regulatory region and the chicken ovalbumin gene, and matrix attachment regions 5' upstream and 3' downstream thereof.
[0027]FIG. 2 schematically illustrates the chicken genomic region having nucleic acid sequence SEQ ID NO: 1, indicating the relative positions and orientations of regions having identity with known domains.
[0028]FIG. 3 illustrates schematically the construction of an expression bacterial artificial chromosome where the insert gene of interest is under the expression control of the chicken ovalbumin promoter. Genes of interest may be inserted into the native translation start site of the ovalbumin gene. L and roman numerals, ovalbumin exons; GOI, gene of interest; start, translation start site; stop, translation stop site; pA, polyadenylation signal; E, EcoR1 site.
[0029]FIG. 4 illustrates an SV40 polyadenylation signal sequence SEQ ID NO: 2.
[0030]FIG. 5 illustrates the nucleotide sequence SEQ ID NO: 3 of a human interferon α2b interferon optimized for expression in an avian cell.
[0031]FIG. 6 illustrates the reconstruction of the chicken genomic region containing the ovalbumin locus.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0032]This description uses gene nomenclature accepted by the Cucurbit Genetics Cooperative as it appears in the Cucurbit Genetics Cooperative Report 18:85 (1995), which are incorporated herein by reference in its entirety. Using this gene nomenclature, genes are symbolized by italicized Roman letters. If a mutant gene is recessive to the normal type, then the symbol and name of the mutant gene appear in italicized lower case letters.
[0033]For convenience, definitions of certain terms employed in the specification, examples, and appended claims are collected here.
Definitions
[0034]The term "avian" as used herein refers to any species, subspecies or race of organism of the taxonomic class ava, such as, but not limited to chicken, turkey, duck, goose, quail, pheasants, parrots, finches, hawks, crows and ratites including ostrich, emu and cassowary. The term includes the various known strains of Gallus gallus, or chickens, (for example, White Leghorn, Brown Leghorn, Barred-Rock, Sussex, New Hampshire, Rhode Island, Ausstralorp, Minorca, Amrox, California Gray, Italian Partidge-colored), as well as strains of turkeys, pheasants, quails, duck, ostriches and other poultry commonly bred in commercial quantities. It also includes an individual avian organism in all stages of development, including embryonic and fetal stages. The term "avian" also may denote "pertaining to a bird", such as "an avian (bird) cell."
[0035]The term "nucleic acid" as used herein refers to any natural or synthetic linear and sequential array of nucleotides and nucleosides, for example cDNA, genomic DNA, mRNA, tRNA, oligonucleotides, oligonucleosides and derivatives thereof. For ease of discussion, such nucleic acids may be collectively referred to herein as "constructs," "plasmids," or "vectors." The term "nucleic acid" further includes modified or derivatized nucleotides and nucleosides such as, but not limited to, halogenated nucleotides such as, but not only, 5-bromouracil, and derivatised nucleotides such as biotin-labeled nucleotides.
[0036]The term "isolated nucleic acid molecule" as used herein refers to a nucleic acid molecule with a structure not identical to a naturally occurring nucleic acid molecule and includes DNA, RNA, or derivatives or variants thereof. The term covers, but is not limited to, (a) a DNA which has the sequence of part of a naturally occurring genomic molecule but is not flanked by at least one of the coding sequences that flank that part of the molecule in the genome of the species in which it naturally occurs; (b) a nucleic acid incorporated into a vector or into the genomic nucleic acid of a prokaryote or eukaryote in a manner such that the resulting molecule is not identical to any vector or naturally occurring genomic DNA; (c) a separate molecule such as a cDNA, a genomic fragment, a fragment produced by polymerase chain reaction (PCR), ligase chain reaction (LCR) or chemical synthesis, or a restriction fragment; (d) a recombinant nucleotide sequence that is part of a hybrid gene, i.e., a gene encoding a fusion protein, and (e) a recombinant nucleotide sequence that is part of a hybrid sequence that is not naturally occurring. Isolated nucleic acid molecules of the present invention can include, for example, natural allelic variants as well as nucleic acid molecules modified by nucleotide deletions, insertions, inversions, or substitutions such that the resulting nucleic acid molecule still essentially encodes an ovalbumin transcriptional regulatory region or a variant thereof of the present invention.
[0037]The terms "polynucleotide," "oligonucleotide," and "nucleic acid sequence" are used interchangeably herein and include, but are not limited to, coding sequences (polynucleotide(s) or nucleic acid sequence(s) which are transcribed and translated into polypeptide in vitro or in vivo when placed under the control of appropriate regulatory or control sequences); control sequences (e.g., translational start and stop codons, promoter sequences, ribosome binding sites, polyadenylation signals, transcription factor binding sites, transcription termination sequences, upstream and downstream regulatory domains, enhancers, silencers, and the like); and regulatory sequences (DNA sequences to which a transcription factor(s) binds and alters the activity of a gene's promoter either positively (induction) or negatively (repression)). No limitation as to length or to synthetic origin are suggested by the terms described above.
[0038]As used herein the terms "peptide," "polypeptide" and "protein" refer to a polymer of amino acids in a serial array, linked through peptide bonds. A "peptide" typically is a polymer of at least two to about 30 amino acids linked in a serial array by peptide bonds. The term "polypeptide" includes proteins, protein fragments, protein analogues, oligopeptides and the like. The term "polypeptides" contemplates polypeptides as defined above that are encoded by nucleic acids, produced through recombinant technology (isolated from an appropriate source such as a bird), or synthesized. The term "polypeptides" further contemplates polypeptides as defined above that include chemically modified amino acids or amino acids covalently or noncovalently linked to labeling moieties.
[0039]The term "fragment" as used herein refers to any isolated portion of the subject nucleic acid molecule constructed artificially (e.g., by chemical synthesis) or by cleaving a natural product into multiple pieces, using restriction endonucleases or mechanical shearing, or a portion of a nucleic acid synthesized by DNA polymerase, including by PCR, or any other polymerizing technique well known in the art, or expressed in a host cell by recombinant nucleic acid technology well known to one of skill in the art. The term "fragment" as used herein may also refer to an isolated portion of a polypeptide, wherein the portion of the polypeptide is cleaved from a naturally occurring polypeptide by proteolytic cleavage by at least one protease, or is a portion of the naturally occurring polypeptide synthesized by chemical or recombinant methods well known to one of skill in the art.
[0040]The terms "recombinant nucleic acid" and "recombinant DNA" as used herein refer to combinations of at least two nucleic acid sequences that are not naturally found in a eukaryotic or prokaryotic cell. The nucleic acid sequences may include, but are not limited to, nucleic acid vectors, gene expression regulatory elements, origins of replication, suitable gene sequences that when expressed confer antibiotic resistance, protein-encoding sequences and the like. The term "recombinant polypeptide" is meant to include a polypeptide produced by recombinant DNA techniques. A recombinant polypeptide may be distinct from a naturally occurring polypeptide either in its location, purity or structure. Generally, a recombinant polypeptide will be present in a cell in an amount different from that normally observed in nature.
[0041]The term "gene" or "genes" as used herein refers to nucleic acid sequences that encode genetic information for the synthesis of a whole RNA, a whole protein, or any portion of such whole RNA or whole protein. Genes that are not naturally part of a particular organism's genome are referred to as "foreign genes," "heterologous genes" or "exogenous genes" and genes that are naturally a part of a particular organism's genome are referred to as "endogenous genes". The term "gene product" refers to an RNA or protein that is encoded by the gene. "Endogenous gene products" are RNAs or proteins encoded by endogenous genes. "Heterologous gene products" are RNAs or proteins encoded by "foreign, heterologous or exogenous genes" and are, therefore, not naturally expressed in the cell.
[0042]The term "expressed" or "expression" as used herein refers to the transcription from a gene to give an RNA nucleic acid molecule at least complementary in part to a region of one of the two nucleic acid strands of the gene. The term "expressed" or "expression" as used herein may also refer to the translation from an RNA molecule to give a protein, a polypeptide or a portion thereof.
[0043]As used herein, the term "locus" refers to the site of a gene on a chromosome. In diploid organisms, pairs of genes control hereditary traits, each in the same position on a pair of chromosomes. These gene pairs, or alleles, may both be dominant or both be recessive in expression of that trait. In either case, the individual is said to be homozygous for the trait controlled by that gene pair. If the gene pair (alleles) consists of one dominant and one recessive trait, the individual is heterozygous for the trait controlled by the gene pair.
[0044]The term "operably linked" refers to an arrangement of elements wherein the components so described are configured so as to perform their usual function. Control sequences operably linked to a coding sequence are capable of effecting the expression of the coding sequence. The control sequences need not be contiguous with the coding sequence, so long as they function to direct the expression thereof. For example, intervening untranslated yet transcribed sequences can be present between a promoter sequence and the coding sequence and the promoter sequence can still be considered "operably linked" to the coding sequence.
[0045]The term "transcription regulatory sequences" as used herein refers to nucleotide sequences that are associated with a gene nucleic acid sequence and which regulate the transcriptional expression of the gene. Exemplary transcription regulatory sequences include enhancer elements, hormone response elements, steroid response elements, negative regulatory elements, and the like.
[0046]The term "promoter" as used herein refers to the DNA sequence that determines the site of transcription initiation by an RNA polymerase. A "promoter-proximal element" is a regulatory sequence generally within about 200 base pairs of the transcription start site.
[0047]The term "matrix attachment region" as used herein refers to a region of a eukaryotic genomic DNA that can be bound to chromosomal scaffold proteins. Matrix (scaffold) attachment regions (MARs) are generally located between transcription units such that the transcription units are within chromosomal loops. The bases of the loops are connected to the scaffold proteins through the MAR at each base. MARs and MAR-like homologs are identified as several recognizable nucleic acid sequences including, but not limited to, TG-rich spans, AT-rich regions and consensus sequences as described by Wang et al, J. Biol. Chem. 270:23239-23242 (1995). MARs may be identified by using suitable software such as, for example, MAR-WIZ® (Futuresoft, Michigan, USA)
[0048]The term "internal ribosome entry sites (IRES)" as used herein refers to a region of a nucleic acid, most typically an RNA molecule, wherein eukaryotic initiation of protein synthesis occurs far downstream of the 5' end of the RNA molecule. A 43S pre-initiation complex comprising the elf2 protein bound to GTP and Met-tRNAi.sup.Met, the 40S ribosomal subunit, and faction elf3 and 31f1A may bind to an "IRES" before locating an AUG start codon. An "IRES" may be used to initiate translation of a second coding region downstream of a first coding region, wherein each coding region is expressed individually, but under the initial control of a single upstream promoter. An "IRES" may be located in a eukaryotic cellular mRNA.
[0049]The term "coding region" as used herein refers to a continuous linear arrangement of nucleotides which may be translated into a polypeptide. A full length coding region is translated into a full length protein; that is, a complete protein as would be translated in its natural state absent any post-translational modifications. A full length coding region may also include any leader protein sequence or any other region of the protein that may be excised naturally from the translated protein.
[0050]The terms "complementary", "complementarity" or "complement" as used herein refers to two nucleic acid molecules that can form specific interactions with one another to form a base-paired double helix.
[0051]The term "probe" as used herein, when referring to a nucleic acid, refers to a nucleotide sequence that can be used to anneal or hybridize with and thereby identify the presence of a complementary sequence, or a complementary sequence differing from the probe sequence but not to a degree that prevents hybridization under the hybridization stringency conditions used. The probe may be modified with labels such as, but not only, radioactive groups, biotin, and the like that are well known in the art.
[0052]The term "hybridizing under stringent conditions" as used herein refers to annealing a first nucleic acid to a second nucleic acid under stringent conditions as defined below. Stringent hybridization conditions typically permit the hybridization of nucleic acid molecules having at least 70% nucleic acid sequence complementarity with the nucleic acid molecule being used as a probe in the hybridization reaction, e.g., high temperature and/or low salt content that tend to disfavor hybridization of dissimilar nucleotide sequences. Alternatively, hybridization of the first and second nucleic acid may be conducted under reduced stringency conditions, e.g., low temperature and/or high salt content that tend to favor hybridization of dissimilar nucleotide sequences. Low stringency hybridization conditions may be followed by high stringency conditions or intermediate medium stringency conditions to increase the selectivity of the binding of the first and second nucleic acids. The hybridization conditions may further include reagents such as, but not limited to, dimethyl sulfoxide (DMSO) or formamide to disfavor still further the hybridization of dissimilar nucleotide sequences. A suitable hybridization protocol may, for example, involve hybridization in 6×SSC (wherein 1×SSC comprises 0.015 M sodium citrate and 0.15 M sodium chloride), at 65° Celsius in an aqueous solution, followed by washing with 1×SSC at 65° Celsius. Formulae to calculate appropriate hybridization and wash conditions to achieve hybridization permitting 30% or less mismatch between two nucleic acid molecules are disclosed, for example, in Meinkoth et al., 1984, Anal. Biochem. 138: 267-284; the content of which is incorporated herein by reference in its entirety. Protocols for hybridization techniques are well known to those of skill in the art and standard molecular biology manuals may be consulted to select a suitable hybridization protocol without undue experimentation. See, for example, Sambrook et al., 1989, "Molecular Cloning: A Laboratory Manual", 2nd ed., Cold Spring Harbor Press, the contents of which are herein incorporated by reference in its entirety.
[0053]Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) from about pH 7.0 to about pH 8.3 and the temperature is at least about 30° Celsius for short probes (e.g., 10 to 50 nucleotides) and at least about 60° Celcius for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulphate) at 37° Celsius, and a wash in 1× to 2×SSC at 50 to 55° Celsius. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 M NaCl, 1% SDS at 37° Celsius, and a wash in 0.5× to 1×SSC at 55 to 60° Celsius. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° Celsius, and a wash in 0.1×SSC at 60 to 65° Celsius.
[0054]The terms "percent sequence identity" as used herein refers to the degree of sequence identity between two nucleic acid sequences or two amino acid sequences as determined using the algorithm of Karlin & Attschul, 1990, Proc. Natl. Acad. Sci. 87: 2264-2268, modified as in Karlin & Attschul, 1993, Proc. Natl. Acad. Sci. 90: 5873-5877. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Attschul et al., 1990, J. Mol. Biol. Q15: 403-410. BLAST nucleotide searches are performed with the NBLAST program, score=100, wordlength=12, to obtain nucleotide sequences homologous to a nucleic acid molecule of the invention. BLAST protein searches are performed with the XBLAST program, score=50, wordlength=3, to obtain amino acid sequences homologous to a reference polypeptide. To obtain gapped alignments for comparison purposes, Gapped BLAST is utilized as described in Attschul et al., 1997, Nucl. Acids Res. 25: 3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (e.g. XBLAST and NBLAST) are used. Other algorithms, programs and default settings may also be suitable such as, but not only, the GCG-Sequence Analysis Package of the U.K. Human Genome Mapping Project Resource Centre that includes programs for nucleotide or amino acid sequence comparisons.
[0055]The terms "vector" or "nucleic acid vector" as used herein refer to a natural or synthetic single or double stranded plasmid or viral nucleic acid molecule (RNA or DNA) that can be transfected or transformed into cells and replicate independently of, or within, the host cell genome. The term "expression vector" as used herein refers to a nucleic acid vector that comprises a transcription regulatory region operably linked to a site wherein is, or can be, inserted, a nucleotide sequence to be transcribed and, optionally, to be expressed, for instance, but not limited to, a sequence coding at least one polypeptide.
[0056]The term "transfection" as used herein refers to the process of inserting a nucleic acid into a host cell. Many techniques are well known to those skilled in the art to facilitate transfection of a nucleic acid into an eukaryotic cell. These methods include, for instance, treating the cells with high concentrations of salt such as a calcium or magnesium salt, an electric field, detergent, or liposome mediated transfection, to render the host cell competent for the uptake of the nucleic acid molecules, and by such methods as micro-injection into a pro-nucleus, sperm-mediated and restriction-mediated integration.
[0057]The terms "recombinant cell" and "genetically transformed cell" refer to a cell comprising a combination of nucleic acid segments not found in a single cell with each other in nature. A new combination of nucleic acid segments can be introduced into an organism using a wide array of nucleic acid manipulation techniques available to those skilled in the art. A recombinant cell can be a prokaryotic cell, or a eukaryotic cell, such as, but not limited to, an avian cell. The recombinant cell may harbor a vector that is extragenomic, i.e. that does not covalently insert into the cellular genome, including a non-nuclear (e.g. mitochondrial) genome(s). A recombinant cell may further harbor a vector or a portion thereof that is intragenomic, i.e. covalently incorporated within the genome (including non-nuclear genome(s)) of the recombinant cell.
[0058]As used herein, a "transgenic avian" is any avian, as defined above, including the chicken, in which one or more of the cells of the avian contain heterologous nucleic acid introduced by manipulation, such as by transgenic techniques. The nucleic acid may be introduced into a cell, directly or indirectly, by introduction into a precursor of the cell by way of deliberate genetic manipulation, such as by microinjection or by infection with a recombinant virus. Genetic manipulation also includes classical cross-breeding, or in vitro fertilization. A recombinant DNA molecule may be integrated within a chromosome, or it may be extrachromosomally replicating DNA.
[0059]The terms "chimeric animal" or "mosaic animal" are used herein to refer to animals in which the recombinant gene is found, or in which the recombinant is expressed, in some but not all cells of the animal. The term "tissue-specific chimeric animal" indicates that the recombinant gene is present and/or expressed in some tissues but not others. As used herein, the term "transgene" means a nucleic acid sequence that is partly or entirely heterologous, i.e., foreign, to the transgenic animal or cell into which it is introduced, or, is homologous to an endogenous gene of the transgenic animal or cell into which it is introduced, but which is designed to be inserted, or is inserted, into the animal's genome in such a way as to alter the genome of the cell into which it is inserted (e.g., it is inserted at a location which differs from that of the natural gene or its insertion results in a knockout).
[0060]The term "chromosomal positional effect" as used herein refers to the variation in the degree of gene transcription as a function of the location of the transcribed locus within the cell genome. Random transgenesis may result in a transgene being inserted at different locations in the genome so that individual cells of a population of transgenic cells may each have at least one transgene, each at a different location and therefore each in a different genetic environment. Each cell, therefore, may express the transgene at a level specific for that particular cell and dependent upon the immediate genetic environment of the transgene.
[0061]In a transgenic animal, as a consequence, different tissues may exhibit different levels of transgene expression. The term "reduced chromosomal positioning effect" as used herein refers to a decreased intercellular variation in the level of gene transcription because of a reduction in the number of sites of insertion of a heterologous nucleic acid molecule into the genome of a recipient cell. Consequently, a reduced chromosomal positioning effect provides a more uniform population of genetically transformed cells with respect to transgene insertion sites in the cellular genomes. In transgenic animals, different tissues may exhibit reduced variability in the levels of transgene expression.
[0062]The term "cytokine" as used herein refers to any secreted polypeptide that affects a function of cells and modulates an interaction between cells in the immune, inflammatory or hematopoietic response. A cytokine includes, but is not limited to, monokines and lymphokines. Examples of cytokines include, but are not limited to, interferon α2b, Interleukin-1 (IL-1), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Tumor Necrosis Factor-α (TNF-α) and Tumor Necrosis Factor-β (TNF-β).
[0063]The term "antibody" as used herein refers to polyclonal and monoclonal antibodies and fragments thereof, and immunologic binding equivalents thereof. Antibodies may include, but are not limited to polyclonal antibodies, monoclonal antibodies (mAbs), humanized or chimeric antibodies, single chain antibodies, Fab fragments, F(ab')2 fragments, fragments produced by a Fab expression library, anti-idiotypic (anti-Id) antibodies, and epitope-binding fragments of any of the above.
[0064]The term "immunoglobulin polypeptide" as used herein refers to a constituent polypeptide of an antibody or a polypeptide derived therefrom. An "immunological polypeptide" may be, but is not limited to, an immunological heavy or light chain and may include a variable region, a diversity region, joining region and a constant region or any combination, variant or truncated form thereof. The term "immunological polypeptides" further includes single-chain antibodies comprised of, but not limited to, an immunoglobulin heavy chain variable region, an immunoglobulin light chain variable region and optionally a peptide linker.
[0065]Techniques useful for isolating and characterizing the nucleic acids and proteins of the present invention are well known to those of skill in the art and standard molecular biology and biochemical manuals may be consulted to select suitable protocols without undue experimentation. See, for example, Sambrook et al., 1989, "Molecular Cloning: A Laboratory Manual", 2nd ed., Cold Spring Harbor, the content of which is herein incorporated by reference in its entirety.
Abbreviations
[0066]Abbreviations used in the present specification include the following: aa, amino acid(s); bp, base pair(s); kb, kilobase; cDNA, DNA complementary to RNA; SSC, sodium chloride-sodium citrate; DMSO, dimethyl sulfoxide; MAR, matrix attachment region; CPE, chromosomal positioning effect; BAC, bacterial artificial chromosome; YAC, yeast artificial chromosome.
[0067]The present invention provides novel isolated and recombinant nucleic acid molecules comprising an avian ovalbumin transcriptional regulatory region and at least one MAR element, which are useful as vectors for inserting a heterologous nucleic acid molecule into the genome of a recipient avian cell. The novel isolated nucleic acid molecules of the present invention are particularly useful for directing the incorporation of a heterologous nucleic acid that is under transcriptional regulation of an avian ovalbumin gene promoter, into the genome of a recipient avian cell while reducing or avoiding chromosomal positioning effects that would otherwise result from randomly distributed insertions of the heterologous nucleic acid molecule into the recipient avian genome. The present invention further provides methods of delivering a heterologous nucleic acid under the transcriptional regulation of an avian ovalbumin transcriptional regulatory region, to an avian cell, whereby the heterologous nucleic acid desired to be expressed under the associated avian ovalbumin gene transcriptional regulatory element can be integrated into an avian cell genome. As well as providing recombinant nucleic acids, vectors and derivatives thereof, the present invention provides transfected and transgenic avian cells and birds derived therefrom that are capable of producing a heterologous polypeptide in the serum or the white of a laid egg.
Nucleic Acids Comprising the Chicken Ovalbumin Zene and 5' and 3' MAR Elements
[0068]The novel isolated and recombinant nucleic acid molecules of the present invention comprise the chicken ovalbumin gene comprising transcriptional regulatory elements positioned 5' upstream of the ovalbumin-encoding region of the native chicken ovalbumin locus and which are necessary for the regulated expression of a downstream polypeptide-encoding nucleic acid, and at least one MAR element.
[0069]The inclusion of a MAR element, and preferably at least two MARs, in the same nucleic acid and flanking the ovalbumin gene region, may confer positional independence to a transfected gene operably linked to the ovalbumin transcriptional regulatory region. While not wishing to be bound by any one theory, it is believed that the 5' and 3' MARs of a transfected nucleic acid molecule of the present invention restrict the number of possible transgene insertion sites within the genome of the recipient avian cell, thereby reducing chromosomal positioning effects upon transcription levels. Thus the isolated novel nucleic acid molecules of the present invention are useful for reducing the chromosomal positional effects exerted on heterologous transgene expression. The heterologous transgene will be operably linked to the ovalbumin transcriptional regulatory region within a novel recombinant nucleic acid molecule transfected into a recipient avian cell. Included in the nucleic acid molecules of the present invention are a region of the avian genome encompassing a MAR upstream of the ovalbumin locus and cis-regulatory elements that may allow gene expression in a tissue-specific manner. The ovalbumin promoter region of the novel nucleic acid molecules is especially useful for directing expression of an operably linked heterologous nucleic acid in a transfected avian cell such as an avian oviduct cell.
[0070]Also within the scope of the present invention that nucleic acid molecules further comprising a region of the chicken ovalbumin locus that is 3' of the ovalbumin-encoding region, or of a nucleic acid insert encoding a heterologous polypeptide. The ovalbumin 3' domain included in the nucleic acid molecules of the present invention includes at least one nucleic acid sequence encoding a 3' MAR element which may cooperate with a 5' MAR to limit the number of sites of insertion into the genome of an avian cell of a transfected nucleic acid molecule. In either event, the directed insertion induced by one or more MARs can reduce or eliminate chromosomal positioning effects, resulting in a more uniform level of gene expression of the heterologous nucleic acid insert in a population of genetically transformed cells.
(a) Isolated Nucleic Acid Encompassing the Chicken Ovalbumin Gene
[0071]One aspect of the present invention, therefore, is a nucleic acid molecule isolated from the genome of a chicken and comprising a proximal ovalbumin promoter suitable for directing transcription of regulation of a transcript encoding ovalbumin, and 5' and 3' MAR elements flanking the ovalbumin gene region.
[0072]BACs 120 and 77 containing overlapping regions of the chicken genome, were sequenced and compiled as the contiguous sequence SEQ ID NO: 1. BAC 120 includes the sequence from nucleotide position 1 to position 157354 of SEQ ID NO: 1. The sequence of BAC 77 begins at nucleotide position 157355 of SEQ ID NO: 1 to position 195102. The nucleic sequence of the 195,102 bp chicken genomic region SEQ ID NO: 1 is shown in FIG. 1. A schematic showing identifiable domains within SEQ ID NO: 1 that have sequence identity or homology to known domain families or previously identified genes mainly identified using BLAST, GenScan and MARWIZ software is shown in FIG. 2. BAC 26, constructed as described in Example 1 below and containing the entire nucleic acid insert SEQ ID NO: 1 less about 11.5 kb at the extreme 5' end, was deposited with American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, Va. 20110, as ATCC No. PTA-5548 on Sep. 24, 2003, under the conditions set forth in the Budapest Treaty.
[0073]The nucleic acid molecule SEQ ID NO: 1 of the present invention has at least four MAR elements. One MAR element is 5' upstream of the ovalbumin gene, between about nucleotide positions 41701 and 41900. MAR-like elements are also between nucleotide positions 56001-56201, 56501-56901, 58401-58701, 76251-76451 and 80151-80451. Another MAR element is between about 96401-96800. MAR elements located 3' downstream of the ovalbumin gene are at nucleotide positions about 144651-144850, about 150601-151600, about 156681-157181, about 157081-15781, about 163701-164100, about 186201-186590 and about 190101-190800 of SEQ ID NO: 1. The chicken ovalbumin gene ATG start codon is at nucleotide position 133372.
[0074]Also dispersed along the nucleic acid molecule represented by SEQ ID NO: 1 are other identifiable domains listed, for example, in Table 2 below, including several serpin- or serpin-like encoding genes, cis transcription regulatory elements of both the serpin-like and ovalbumin genes, and at least two other, putatively functional genes, X and Z. Between the various domains, genes or other elements are stretches of nucleotides, the functions of which may serve to maintain the position and configuration of the elements relative to each other.
[0075]The isolated nucleic acids of the present invention and derivatives and truncated variants thereof may be incorporated into a vector, such as a bacterial or yeast artificial chromosome. The BAC cloning system (Shizuya et al, Proc. Natl. Acad. Sci (U.S.A.), 89:8794:8797, (1992) has been developed to stably maintain large fragments of genomic DNA (100-300 kb) in E. coli. An exemplary BAC vector consists of the pBeloBAC11 vector that has been described by Kim et al, Genomics, 34:213:218 (1996). Genomic DNA can be partially digested, for example, using enzymes that permit ligation into either the BamH I or Hind III sites in the vector. It is contemplated that any suitable restriction sites may be used that are useful for incorporating genomic DNA into a selected BAC vector. Flanking these cloning sites are T7 and SP6 RNA polymerase transcription initiation sites that can be used to generate end probes by either RNA transcription or PCR methods. BAC DNA is purified from the host cell as a supercoiled circle. Converting these circular molecules into a linear form precedes both size determination and introduction of the BACs into recipient avian cells. A suitable cloning site may be flanked, for example, by two Not I restriction sites, permitting cloned segments to be excised from the vector by Not I digestion. Alternatively, the BAC vector may be linearized by treatment with the commercially available enzyme lambda terminase that leads to the cleavage at a cosN site. However, this cleavage method results in a full length BAC clone containing both the insert DNA and the BAC vector sequences.
[0076]One embodiment of the novel isolated nucleic acid molecules of the present invention, therefore, is an isolated chicken nucleic acid molecule encoding an ovalbumin transcriptional regulatory region and a 5' MAR. In one embodiment of the present invention, the novel isolated nucleic acid molecule further comprises a 3' MAR downstream of the ovalbumin gene. The isolated nucleic acid molecules of the present invention may also include nucleic acid elements such as, but not limited to, a transcription enhancer element, a negative regulator element, a hormone responsive element, an avian CR1 repeat element that together may constitute, in whole or in part, the ovalbumin transcriptional regulatory region, a proximal ovalbumin promoter and a signal peptide-encoding region. There are also stretches of nucleic acid between these constituent elements that organize the various elements into an ordered linear array. While the constituent elements of the ovalbumin transcriptional control region are preferably ordered as in sequence SEQ ID NO: 1, it is within the scope of the present invention for the cis-elements of the ovalbumin transcriptional regulatory region to be in any linear arrangement that will allow the formation of a transcript comprising the nucleotide sequence, or its complement, of a nucleic acid insert operably linked to the ovalbumin transcriptional regulatory region.
[0077]The novel isolated nucleic acid molecules of the present invention allow one skilled in the art to, for example, (a) make copies of those nucleic acid molecules by procedures such as, but not limited to, insertion into a cell for replication by the cell, by chemical synthesis or by procedures such as PCR or LCR, (b) obtain nucleic acid molecules which include at least a portion of such nucleic acid molecules, including full-length genes, full-length coding regions, transcriptional regulatory sequences, truncated coding regions and the like, (c) identify and obtain ovalbumin transcriptional regulatory region homologs found in other avian species such as, but not limited to, turkey, duck, goose, quail, pheasant, parrot, finch, ratites including ostrich, emu and cassowary and, (d) to obtain isolated nucleic acids capable of hybridizing to an avian ovalbumin transcriptional regulatory region nucleic acid and of being used as a probe to detect the presence of nucleic acid-related sequences by complementation between the probe and the target nucleic acid.
[0078]Such nucleic acid homologs can be obtained in a variety of ways including using traditional cloning techniques to screen appropriate libraries, amplifying appropriate libraries or DNA using oligonucleotide primers derived from the novel nucleic acid molecules of the present invention in a polymerase chain reaction or other amplification method, and screening public and/or private databases containing genetic sequences using nucleic acid sequences of the present invention to identify targets. Examples of preferred libraries to screen, or from which to amplify nucleic acid molecules, include but are not limited to avian BAC libraries, genomic DNA libraries, and cDNA libraries. Similarly, preferred sequence databases useful for screening to identify sequences in other species homologous to chicken ovalbumin transcriptional regulatory region include, but are not limited to, GenBank and the mammalian Gene Index database of The Institute of Genomics Research (TIGR).
[0079]Nucleotides used to construct the nucleic acids of the present invention can be labeled to provide a signal as a means of detection, using conventional labeling technologies such as radioactive labels, fluorescent compounds, enzymes and chemiluminescent moieties. Methods useful in selecting appropriate labels and binding protocols for binding the labels to the synthetic nucleotides are well known to those of skill in the art.
[0080]In one embodiment of the isolated nucleic acid molecule according to the present invention, the nucleic acid is isolated from a chicken.
[0081]In other embodiments of the isolated nucleic acid molecule according to the present invention, the nucleic acid molecule comprises a nucleotide sequence having at least 80% identity, at least 95% identity or at least 99% identity to the nucleotide sequence according to SEQ ID NO: 1, or the complement thereof.
[0082]In other embodiments, the isolated nucleic acid molecule of the invention comprises the nucleotide sequence according to SEQ ID NO: 1 or has the nucleotide sequence according to SEQ ID NO: 1. In another embodiment, the isolated nucleic acid molecule can be an allelic variant of SEQ ID NO: 1.
(b) Fragments and Variants of SEQ ID NO: 1
[0083]Fragments of the isolated nucleic acid molecules of the present invention also are within the scope of the present invention. As used herein, a fragment of a nucleic acid molecule refers to a nucleotide sequence having fewer nucleotides than the nucleotide sequence SEQ ID NO: 1 but which includes a nucleic acid sequence of the ovalbumin transcriptional regulatory region able to direct and regulate transcription of a nucleic acid, and at least one MAR element.
[0084]The isolated nucleic acid molecule having the sequence SEQ ID NO: 1 may be reduced in size by truncating regions that do not affect the expression of a heterologous nucleic acid placed under the transcriptional control of the ovalbumin transcription regulatory region. A truncated variant of the nucleic acid molecule of the present invention is understood to be any variant of SEQ ID NO: 1 less nucleotides at either the 5' and/or the 3' end of SEQ ID NO: 1. For example, it is contemplated that any of the nucleotides from positions 1-about 40500 may be individually, in part, or in total, deleted from the variant nucleic acid molecule. Similarly, nucleotides from positions about 151700, 164200, 186690 or 190900 to 195101 of the nucleic acid molecule having sequence SEQ ID NO: 1 may be removed, retaining 1, 2, 3, 4 or more of the MAR or MAR-like elements respectively located 3' at the chicken ovalbumin gene. Useful truncated variants of SEQ ID NO: 1, therefore, include, but are not limited to, from base position about 41000 to about 191500, to about 187000, to about 164500, to about 152000, or to about 145500 and from base position about 96000 to about 191500, to about 187000, to about 164500, to about 152000 or to about 145500. Other useful truncated variants of SEQ ID NO: 1 include regions from nucleotide positions about 56000, about 58350, about 76200 and about 80000 to about 191500, to about 187000, to about 164500, to about 152000 or to about 145500.
[0085]Therefore, the invention encompasses nucleic acid molecules which do not include regions that do not contribute to the desired functionality of inserting a heterologous nucleic acid into an avian genome with reduced or no chromosomal positioning effect. The region 5' upstream of the MAR located at nucleotide positions 41701-41900 of SEQ ID NO: 1, may be deleted to give a truncated variant of SEQ ID NO: 1. For example, the approximately 11.5 kb region extending from nucleotide position 1 of SEQ ID NO: 1 not present in BAC 26 may be deleted. Likewise, it is contemplated that other regions of SEQ ID NO: 1 as listed in Table 2, such as encoding the serpin-like proteins, may be selectively deleted.
Recombinant Nucleic Acids
[0086]Another aspect of the present invention is recombinant nucleic acid molecules comprising at least one, and preferably at least two, avian MARs and an avian ovalbumin transcription regulatory region, including the proximal promoter thereof. The recombinant nucleic acid molecules of the present invention are particularly useful for delivering a desired heterologous nucleic acid to a recipient avian cell while reducing chromosomal positional effects upon transcription from the integrated heterologous nucleic acid. It is contemplated that regions of SEQ ID NO: 1 may be omitted from the recombinant nucleic acid molecules of the present invention without substantially affecting the reduction in the CPE compared to a similar nucleic acid molecule not including MAR elements. For example, one or more of the serpin-encoding regions of SEQ ID NO: 1 listed in Table 2, below, may not be included.
[0087]The present invention, therefore, provides recombinant nucleic acid molecules that comprise at least one avian MAR and an avian ovalbumin transcription regulatory region optionally operably linked in a linear array to a selected heterologous or endogenous polypeptide-encoding nucleic acid insert, and which may express the nucleic acid insert when transfected to a suitable host cell, preferably an avian cell.
[0088]The nucleic acid insert, such as a heterologous nucleic acid can be operably linked 3' downstream of the ovalbumin proximal promoter and is thereby expressed as an RNA transcript by a transfected recipient cell. The heterologous nucleic acid may be inserted into the recombinant nucleic acid of the present invention 3' downstream of a region encoding a peptide leader region so that a heterologous polypeptide encoded by the inserted nucleic acid may include this leader region. It is within the scope of the present invention for the recombinant nucleic acid to have the nucleic acid insert encoding the desired polypeptide to be operably inserted into the ovalbumin coding region, or operably replacing the ovalbumin coding region in whole or in part. The generation of BACs comprising a heterologous nucleic acid under the transcriptional control of the ovalbumin gene control region according to the present invention are described in Examples 2 and 3, below.
[0089]To increase the efficiency of expression of the heterologous nucleic acid insert, a polyadenylation signal region may be included at the 3' end of the inserted nucleic acid to allow the transcript directed by the novel ovalbumin transcriptional regulatory region to proceed beyond the nucleic acid insert encoding a selected polypeptide thereby providing a transcript further comprising a 3' untranslated region and a polyadenylated tail. Any suitable functional polyadenylation signal sequence may be linked to the 3' end of the nucleic acid insert including, for example, the SV40 polyadenylation signal sequence SEQ ID NO: 2 as shown in FIG. 4, bovine growth hormone adenylation sequence or the like. It is further anticipated that the recombinant nucleic acid molecules of the present invention may comprise the chicken ovalbumin 3' domain, or a variant thereof. The ovalbumin 3' domain may comprise the ovalbumin 3' untranslated region, an ovalbumin gene polyadenylation sequence and at least one of the 3' MAR elements identified downstream of the ovalbumin-encoding region of SEQ ID NO: 1. If the heterologous nucleic acid is inserted within the ovalbumin encoding region, and in-phase with the ovalbumin gene, the polyadenylation signal region of the ovalbumin gene may be used.
[0090]In one embodiment of the recombinant nucleic acid molecule according to the present invention, the recombinant nucleic acid molecule comprises the nucleotide sequence according to SEQ ID NO: 1, or the complement thereof.
[0091]Another aspect of the present invention is a recombinant DNA molecule comprising a MAR element and an avian ovalbumin transcriptional regulatory region. In one embodiment, the ovalbumin transcriptional regulatory region is operably linked in linear array to a nucleic acid insert encoding a polypeptide sought to be expressed, and a polyadenylation signal sequence optionally operably linked thereto. It is contemplated that when the recombinant nucleic acid molecule is to be delivered to a recipient avian cell for expression therein, the sequence of the inserted heterologous nucleic acid sequence may be modified so that the codons thereof are optimized for the codon usage of the recipient species as described below.
[0092]In a preferred embodiment, a MAR element is located 5' upstream of the ovalbumin transcriptional regulatory region. Suitable MAR elements, for example, are located at about nucleotide positions about 41701-41800 and about 96401-96800 of sequence SEQ ID NO: 1.
[0093]In one embodiment of the present invention, the recombinant nucleic acid molecule comprises the nucleotide sequence from nucleotides position about 40750 to 195101 of SEQ ID NO: 1. Various embodiments of the recombinant nucleic acid molecules of the present invention comprise a 5' MAR and/or a 3' MAR, and the ovalbumin transcriptional regulatory region. In one embodiment, the recombinant nucleic acid further comprises a T gene. In others, the recombinant nucleic acid further comprises at least one nucleic acid region selected from the group consisting of the U serpin gene, a V serpin gene, an X gene, a Y gene and a Z serpin derived from SEQ ID NO: 1.
[0094]Another embodiment of the recombinant nucleic acid molecules further comprises at least one avian MAR 3' downstream of the nucleic acid insert. Suitable MAR elements for inclusion 3' downstream of the ovalbumin transcriptional regulatory region of the recombinant construct of the present invention are found at nucleotide positions about 144651-144850, about 150800-151600, about 163701-164100, about 186201-186590 and about 190101-190800 of sequence SEQ ID NO: 1. In one embodiment of the recombinant nucleic acid molecules, the ovalbumin transcriptional regulatory region, the avian 5' MAR, and the avian 3' MAR are independently capable of hybridizing under high stringency conditions to the nucleic acid sequence according to SEQ ID NO: 1, or the complement thereof.
[0095]In various embodiments of the present invention, the recombinant nucleic acid molecule is inserted into a vector such as, but not limited to, a plasmid or viral vector.
[0096]Other embodiments of the recombinant nucleic acid molecules further comprise a plasmid or viral origin of replication. In one embodiment, the recombinant nucleic acid molecule is a bacterial or yeast artificial chromosome.
[0097]Yet another embodiment of the recombinant nucleic acid molecule according to the present invention, therefore, is a recombinant nucleic acid molecule comprising an avian ovalbumin transcription regulatory region, an avian 5' MAR, a heterologous nucleic acid encoding a heterologous polypeptide desired to be expressed by a recipient genetically modified cell, a polyadenylation signal sequence, and an avian 3' MAR, wherein the avian ovalbumin transcription regulatory region, 5' MAR, and the 3' MAR each independently hybridizes under high stringency conditions to the nucleic acid sequence SEQ ID NO: 1, or a complement thereof.
[0098]Polypeptide expression under the control of an avian ovalbumin promoter
[0099]Another aspect of the present invention of the novel isolated ovalbumin transcriptional regulatory region is increasing the amount of a heterologous protein present in a bird (especially the chicken) by gene transfer. Typically, a heterologous polypeptide-encoding nucleic acid insert transferred into the recipient animal host will be operably linked with the ovalbumin transcriptional regulatory region to allow the cell to initiate and continue production of the genetic product protein. A recombinant DNA molecule of the present invention can be transferred into the extra-chromosomal or genomic DNA of the host.
[0100]A useful application of the novel isolated and recombinant nucleic acid molecules of the present invention is to increase the amount of a heterologous protein present in a bird, (especially the chicken) by gene transfer. Typically, a heterologous polypeptide-encoding nucleic acid insert transferred into the recipient bird host or an isolated cell or cell-line from the bird will be operably linked with the ovalbumin transcriptional regulatory region to allow the cell to initiate and continue production of the genetic protein product.
[0101]The isolated nucleic acid molecule SEQ ID NO: 1 is useful for inserting therein a heterologous nucleic acid that is desired to be expressed as a transcript or, ultimately, as a polypeptide. A heterologous nucleic acid may be operably linked to the proximal promoter region of the ovalbumin gene at any position 3' downstream of the promoter that allows transcription from the heterologous nucleic acid and synthesis of the desired encoded peptide.
[0102]Some, or all, of the ovalbumin-encoding region of the isolated or recombinant nucleic acids of the present invention may be replaced by a heterologous nucleic acid to be expressed under the transcriptional control of upstream ovalbumin gene control region. The heterologous nucleic acid may be inserted into the isolated or recombinant nucleic acids of the present invention so that the expressed amino acid sequences derived from the ovalbumin may be linked to the expressed heterologous protein either at the N-terminus or C-terminus thereof.
[0103]Any of the vectors of the present invention may also optionally include a sequence encoding a signal peptide that directs secretion of the protein expressed by the vector from the transgenic cells, for instance, from tubular gland cells of the oviduct. This aspect of the invention effectively broadens the spectrum of exogenous proteins that may be deposited in avian eggs using the methods of the invention. Where an exogenous protein would not otherwise be secreted, the vector bearing the coding sequence is modified to comprise, for instance, about 60 bp encoding a signal peptide. The DNA sequence encoding the signal peptide is inserted in the vector such that the signal peptide is located at the N-terminus of the protein encoded by the vector.
[0104]The expression vectors of the present invention comprise avian ovalbumin transcriptional regulatory regions that can direct expression of either fusion or non-fusion proteins. With fusion vectors, a number of amino acids are usually added to the desired expressed target gene sequence such as, but not limited to, a protein sequence for thioredoxin. A proteolytic cleavage site may further be introduced at a site between the target recombinant protein and the fusion sequence. Additionally, a region of amino acids such as a polymeric histidine region may be introduced to allow binding of the fusion protein to metallic ions such as nickel bonded to a solid support, for purification of the fusion protein. Once the fusion protein has been purified, the cleavage site allows the target recombinant protein to be separated from the fusion sequence. Enzymes suitable for use in cleaving the proteolytic cleavage site include, but are not limited to, Factor Xa and thrombin. Fusion expression vectors that may be useful in the present invention include pGex (Amrad Corp., Melbourne, Australia), pRIT5 (Pharmacia, Piscataway, N.J.) and pMAL (New England Biolabs, Beverly, Mass.), that fuse glutathione S-transferase, protein A, or maltose E binding protein, respectively, to the target recombinant protein.
[0105]The present invention further relates to nucleic acid vectors and transgenes derived therefrom that incorporate polypeptide-encoding regions, wherein a first polypeptide-encoding region is operatively linked to an avian ovalbumin promoter and a second polypeptide-encoding region is operatively linked to an Internal Ribosome Entry Sequence (IRES). It is contemplated that the first polypeptide-encoding region, the IRES and the second polypeptide-encoding region of a recombinant DNA of the present invention may be arranged linearly, with the IRES operably positioned immediately 5' of the second polypeptide-encoding region. This nucleic acid construct, when inserted into the genome of a bird and expressed therein, will generate individual polypeptides that may be post-translationally modified and combined in the white of a hard-shell bird egg. Alternatively, the expressed polypeptides may be isolated from an avian egg and combined in vitro.
[0106]Expression of a heterologous nucleic acid by a recombinant expression vector according to the present invention can be obtained using eukaryotic host cells, preferably avian cells, more preferably chicken cells, and still more preferably chicken oviduct cells, especially tubular gland cells. The use of eukaryotic host cells permit partial or complete post-translational modification such as, but not only, glycosylation and/or the formation of the relevant inter- or intra-chain disulfide bonds. Examples of vectors useful for expression in the chicken Gallus gallus include pYepSec1 as in Baldari et al., E.M.B.O.J., 6, 229-234 (1987) and pYES2 (Invitrogen Corp., San Diego, Calif.), incorporated herein by reference in their entireties.
[0107]One aspect of the present invention is methods of delivering a novel nucleic acid molecule of the present invention to the cytoplasm of an avian cell having a nucleus, thereby generating a transfected and genetically transformed avian cell. Such incorporation can be carried out by the various forms of transfection, depending upon the vector/host cell system. It is contemplated that the incorporation of recombinant nucleic acid molecules of the present invention into a recipient cell may be by any suitable method such as, but not limited to, viral transfer, electroporation, gene gun insertion, sperm-mediated transfer to an ovum, microinjection and the like.
[0108]In the various embodiments of these methods, the avian cell may be a chicken cell or a quail cell. In some embodiments of the methods of the present invention, the avian cell is within oviductal tissue of a bird, an isolated oviduct cell or primary cell line, or a sustainable oviduct cell line. Preferably, the oviduct cells are tubular gland cells.
[0109]Heterologous polypeptide can be produced by transfected cells of the invention in vitro, i.e., in tissue culture outside the body of a living animal. Alternatively, the nucleic acids of the present invention may be delivered to an animal such as a chicken, whereupon the nucleic acid may enter cells and be expressed therein. It is anticipated that the nucleic acids of the present invention may integrate into the genome of the recipient cells and then express the encoded, typically heterologous, polypeptide therein. Preferably, a heterologous nucleic acid is delivered to oviduct cells within a chicken for synthesis of the desired polypeptide and its deposition in the white of an egg.
[0110]Another aspect of the present invention is a eukaryotic cell transfected with an expression vector according to the present invention and described above. For example, in one embodiment, the transformed cell can be a chicken oviduct cell or cell line, including a sustainable cell line, and the transfected nucleic acid insert comprises the chicken ovalbumin transcriptional regulatory region, a 5' MAR and/or a 3' MAR, a nucleic acid insert encoding a human interferon α2b and codon optimized for expression in an avian cell, and an SV40 polyadenylation sequence. In another example, the nucleic acid insert encodes an immunoglobulin heavy chain and a second chain under the transcriptional control of an IRES.
[0111]The transfected cell according to the present invention may be transiently transfected, whereby the transfected recombinant nucleic acid, such as DNA, or expression vector may not be integrated into the genomic nucleic acid. However, the transfected recombinant DNA or expression vector may be stably integrated into the genomic DNA of the recipient cell, thereby replicating with the cell so that each daughter cell receives a copy of the transfected nucleic acid. When the recombinant DNA or expression vector of the present invention is integrated into the genomic DNA of the recipient cell so that the cell is genetically transformed, it is anticipated that the MAR element(s) of the integrated nucleic acid will direct integration a limited number of integration site within the target genome, thereby producing a population of cells more uniform with regard to the level of expression of the heterologous nucleic acid.
[0112]The present invention also includes a transgenic bird producing a heterologous protein expressed from a transfected nucleic acid according to the present invention. The transgenic bird is selected from a turkey, duck, goose, quail, pheasant, ratite, an ornamental bird or a feral bird. In a preferred embodiment, the avian is a chicken and the heterologous protein produced under the transcriptional control of the avian ovalbumin transcriptional regulatory region according to the present invention is produced in the white of an egg.
Viral Host Cell Transformation
[0113]Nucleic acid sequences or derivative or truncated variants thereof, may be introduced into viruses such as an adenovirus or vaccinia virus. Methods for making a viral recombinant vector useful for expressing a protein under the control of the ovalbumin promoter are analogous to the methods disclosed in U.S. Pat. Nos. 4,603,112; 4,769,330; 5,174,993; 5,505,941; 5,338,683; 5,494,807; 4,722,848; Paoletti, E., 1996, Proc. Natl. Acad. Sci. 93: 11349-11353; Moss, 1996, Proc. Natl. Acad. Sci. 93: 11341-11348; Roizman 1996, Proc. Natl. Acad. Sci. 93: 11307-11302; Frolov et al., 1996, Proc. Natl. Acad. Sci. 93: 11371-11377; Grunhaus et al., 1993, Seminars in Virology 3: 237-252 and U.S. Pat. Nos. 5,591,639; 5,589,466; and 5,580,859 relating to DNA expression vectors, inter alia, the contents of which are incorporated herein by reference in their entireties.
[0114]Retrovirus vectors and adeno-associated virus vectors provide efficient systems of delivery of genes into cells, and the transferred nucleic acids may be stably integrated into the chromosomal DNA of the host. Protocols for producing recombinant retroviruses and for infecting cells in vitro or in vivo with such viruses can be found in Ausubel et al., 1989, Current Protocols in Molecular Biology §§ 9.10-9.14 and other standard laboratory manuals. Examples of suitable retroviruses include pLJ, pZIP, pWE and pEM which are well known to those skilled in the art. Examples of suitable packaging virus lines for preparing both ecotropic and amphotropic retroviral systems include psiCrip, psiCre, psi2 and psiAm.
[0115]Furthermore, it is possible to limit the infection spectrum of retroviruses and consequently of retroviral-based vectors, by modifying the viral packaging proteins on the surface of the viral particle (see, for example PCT publications WO 93/25234, WO 94/06920, and WO 94/11524). Roux et al., 1898, Proc. Natl. Acad. Sci. 86:9079-9083; Julan et al., 1992, J. Gen. Virol. 73:3251-3255; and Goud et al., 1983, Virology 163:251-254); Neda et al., 1991, J. Biol. Chem. 266:14143-14146), which are incorporated herein by reference in their entireties.
[0116]One retrovirus for randomly introducing a transgene into the avian genome is a replication-deficient ALV retrovirus. To produce an appropriate ALV retroviral vector, a pNLB vector may be modified by inserting a region comprising at least part of the ovalbumin transcriptional regulatory region, a MAR element and one or more exogenous genes between the 5' and 3' long terminal repeats (LTRs) of the retrovirus genome. Any coding sequence placed in-frame and downstream of the ovalbumin promoter will be expressed at high levels and especially in the tubular gland cells of the oviduct magnum because the ovalbumin promoter drives the high level of expression of the ovalbumin protein and is only active in the oviduct tubular gland cells.
[0117]Another viral gene delivery system useful in the present invention utilizes adenovirus-derived vectors (see, for example, Berkner et al., 1988, BioTechniques 6:616-629; Rosenfeld et al., 1991, Science 252:431-434; and Rosenfeld et al., 1992, Cell 68:143-155), incorporated herein by reference in their entireties. Suitable adenoviral vectors derived from the adenovirus strain Ad type 5 dl324 or other strains of adenovirus (e.g., Ad2, Ad3, Ad7 etc.) are well known to those skilled in the art. Introduced adenoviral DNA (and foreign DNA contained therein) is not integrated into the genome of a host cell but remains episomal, thereby avoiding potential problems that can occur as a result of insertional mutagenesis in situations where introduced DNA becomes integrated into the host genome (e.g., retroviral DNA).
[0118]Yet another viral vector system is the adeno-associated virus (AAV). Vectors containing as little as 300 base pairs of AAV can be packaged and can integrate. In the present invention, at least part of the heterologous nucleic acid will include an operable region of the avian ovalbumin transcriptional regulatory region and a MAR element. An AAV vector such as that described in Tratschin et al., 1985, Mol. Cell. Biol. 5:3251-3260, can be used to introduce DNA into cells.
[0119]Other viral vector systems that may have application in the methods according to the present invention have been derived from, but are not limited to, herpes viruses, vaccinia viruses, avian leucosis viruses and several RNA viruses.
Non-Viral Expression Vectors
[0120]Most non-viral methods of gene transfer rely on normal mechanisms used by eukaryotic cells for the uptake and intracellular transport of macromolecules. In preferred embodiments, non-viral gene delivery systems of the present invention rely on endocytic pathways for the uptake of the subject ovalbumin transcriptional regulatory region and operably linked polypeptide-encoding nucleic acid by the targeted cell. Exemplary gene delivery systems of this type include liposomal derived systems, poly-lysine conjugates, and artificial viral envelopes.
[0121]In a representative embodiment, a nucleic acid comprising the novel recombinant nucleic acids of the present invention can be entrapped in liposomes bearing positive charges on their surface (e.g., lipofectins) and (optionally) which are tagged with antibodies against cell surface antigens of the target tissue (Mizuno et al., 1992, NO Shinkei Geka 20: 547-551; PCT publication WO91/06309; Japanese patent application 1047381; and European patent publication EP-A-43075, all of which are incorporated herein by reference in their entireties).
[0122]In similar fashion, the gene delivery system comprises an antibody or cell surface ligand that is cross-linked with a gene binding agent such as polylysine (see, for example, PCT publications WO93/04701, WO92/22635, WO92/20316, WO92/19749, and WO92/06180, all of which are incorporated herein by reference in their entireties). It will also be appreciated that effective delivery of the subject nucleic acid constructs via receptor-mediated endocytosis can be improved using agents which enhance escape of genes from the endosomal structures. For instance, whole adenovirus or fusogenic peptides of the influenza HA gene product can be used as part of the delivery system to induce efficient disruption of DNA-containing endosomes (Mulligan et al., 1993, Science 260-926; Wagner et al., 1992, Proc. Natl. Acad. Sci. 89:7934-7938; and Christiano et al., 1993, Proc. Natl. Acad. Sci. 90:2122-2126, all of which are incorporated herein by reference in their entireties). It is further contemplated that a recombinant DNA molecule of the present invention may be delivered to a recipient host cell by other non-viral methods including by gene gun, microinjection, sperm-mediated transfer, or the like.
[0123]Another aspect of the present invention is a method of expressing a heterologous polypeptide in a eukaryotic cell by transfecting a cell with a recombinant nucleic acid molecule of the invention, as described above, and culturing the transfected cell under conditions suitable for expression of the heterologous polypeptide under the control of the avian ovalbumin transcriptional regulatory region.
[0124]In one embodiment of this aspect, the nucleic acid molecule is integrated into the genome of the recipient avian cell. In some embodiments the recipient avian oviduct cell is a chicken cell, preferably a chicken oviduct cell, more preferably an oviduct tubular gland cell.
[0125]The protein of the present invention may be produced in purified form by any known conventional techniques. For example, chicken cells, an egg or an egg white may be homogenized and centrifuged. The supernatant may then be subjected to sequential ammonium sulfate precipitation and heat treatment. The fraction containing the protein of the present invention is subjected to gel filtration in an appropriately sized dextran or polyacrylamide column to separate the proteins. If necessary, the protein fraction may be further purified by HPLC or other methods well known in the art of protein purification.
Expression of Heterologous Multimeric Proteins by Transfected Avian Cells
[0126]The present invention provides methods for the production of a multimeric protein by an avian cell, comprising the step of culturing an avian cell transfected with a first expression vector and, optionally, a second expression vector; the expression vectors may each have a transcription unit comprising a nucleotide sequence encoding a first heterologous polypeptide, a transcription promoter, and a transcriptional terminator operatively linked to the nucleotide sequence encoding a second heterologous polypeptide, such that the cultured avian cell produces a multimeric protein comprising the first and second heterologous polypeptides.
[0127]The isolated nucleic acids and recombinant nucleic acid constructs derived therefrom of the present invention are useful to express nucleic acid sequences of polypeptides that are optimized for expression in avian cells, and derivatives and fragments thereof. Such derivatives include, for instance, polypeptides with conservative amino acid replacements, that is, those within a family of amino acids that are related in their side chains (commonly known as acidic, basic, nonpolar, and uncharged polar amino acids). Phenylalanine, tryptophan, and tyrosine are sometimes classified jointly as aromatic amino acids and other groupings are known in the art (see, for example, "Biochemistry", 2nd ed, L. Stryer, ed., WH Freeman and Co., 1981). Peptides in which more than one replacement has taken place can readily be tested for activity in the same manner as derivatives with a single replacement, using conventional polypeptide activity assays (e.g. for enzymatic or ligand binding activities).
[0128]Regarding codon optimization, for example, if the recombinant DNA is transfected into a recipient chicken cell, the sequence of the nucleic acid insert to be expressed is optimized for chicken codon usage. This may be determined from the codon usage of at least one, and preferably more than one, protein expressed in a chicken cell according to well known principles. For example, in the chicken the codon usage may be determined from the nucleic acid sequences encoding the proteins lysozyme, ovalbumin, ovomucin and ovotransferrin of chicken. Optimization of the sequence for codon usage elevates the level of translation in avian eggs.
[0129]One embodiment of the recombinant nucleic acid of the present invention, comprises an insert encodes the human interferon α2b polypeptide. The exemplary nucleic acid sequence SEQ ID NO: 3 (FIG. 5) encodes the polypeptide human interferon α2b in accordance with avian cell codon usage, as determined from the nucleotide sequences encoding chicken ovomucin, ovalbumin, ovotransferrin and lysozyme.
[0130]The invention methods for producing multimeric proteins include immunoglobulins, such as antibodies, and antigen binding fragments thereof. Thus, in one embodiment of the present invention, the multimeric protein is an immunoglobulin, wherein the first and second heterologous polypeptides are an immunoglobulin heavy and light chains respectively. Illustrative examples of this and other aspects of the present invention for the production of heterologous multimeric polypeptides in avian cells are fully disclosed in U.S. patent application Ser. No. 09/877,374, filed Jun. 8, 2001, by Rapp, published as US-2002-0108132-A1 on Aug. 8, 2002, and U.S. patent application Ser. No. 10/251,364, filed Sep. 18, 2002, by Rapp, now U.S. Pat. No. 7,312,374 issued Dec. 25, 2007, both of which are incorporated herein by reference in their entirety.
[0131]Accordingly, the invention further provides immunoglobulin and other multimeric proteins that have been produced by transgenic avians of the invention.
[0132]In various embodiments, an immunoglobulin polypeptide encoded by the transcriptional unit of at least one expression vector may be an immunoglobulin heavy chain polypeptide comprising a variable region or a variant thereof, and may further comprise a D region, a J region, a C region, or a combination thereof. An immunoglobulin polypeptide encoded by an expression vector may also be an immunoglobulin light chain polypeptide comprising a variable region or a variant thereof, and may further comprise a J region and a C region. The present invention also contemplates multiple immunoglobulin regions that are derived from the same animal species, or a mixture of species including, but not only, human, mouse, rat, rabbit and chicken. In preferred embodiments, the antibodies are human or humanized.
[0133]In other embodiments, the immunoglobulin polypeptide encoded by at least one expression vector comprises an immunoglobulin heavy chain variable region, an immunoglobulin light chain variable region, and a linker peptide thereby forming a single-chain antibody capable of selectively binding an antigen.
[0134]Another aspect of the present invention provides a method for the production in an avian of an heterologous protein capable of forming an antibody suitable for selectively binding an antigen. This method comprises a step of producing a transgenic avian incorporating at least one transgene, wherein the transgene encodes at least one heterologous polypeptide selected from an immunoglobulin heavy chain variable region, an immunoglobulin heavy chain comprising a variable region and a constant region, an immunoglobulin light chain variable region, an immunoglobulin light chain comprising a variable region and a constant region, and a single-chain antibody comprising two peptide-linked immunoglobulin variable regions.
[0135]In one embodiment of this method, the isolated heterologous protein is an antibody capable of selectively binding to an antigen which may be generated by combining at least one immunoglobulin heavy chain variable region and at least one immunoglobulin light chain variable region, preferably cross-linked by at least one disulfide bridge. The combination of the two variable regions generates a binding site that binds an antigen using methods for antibody reconstitution that are well known in the art.
[0136]The present invention also encompasses immunoglobulin heavy and light chains, or variants or derivatives thereof, to be expressed in separate transgenic avians, and thereafter isolated from separate media including serum or eggs, each isolate comprising one or more distinct species of immunoglobulin polypeptide. The method may further comprise the step of combining a plurality of isolated heterologous immunoglobulin polypeptides, thereby producing an antibody capable of selectively binding to an antigen. In this embodiment, for instance, two or more individual transgenic avians may be generated wherein one transgenic produces serum or eggs having an immunoglobulin heavy chain variable region, or a polypeptide comprising such, expressed therein. A second transgenic animal, having a second transgene, produces serum or eggs having an immunoglobulin light chain variable region, or a polypeptide comprising such, expressed therein. The polypeptides from two or more transgenic animals may be isolated from their respective sera and eggs and combined in vitro to generate a binding site capable of binding an antigen.
[0137]Examples of therapeutic antibodies that can be used in methods of the invention include but are not limited to HERCEPTIN® (Trastuzumab) (Genentech, CA) which is a humanized anti-HER2 monoclonal antibody for the treatment of patients with metastatic breast cancer; REOPRO® (abciximab) (Centocor) which is an anti-glycoprotein IIb/IIIa receptor on the platelets for the prevention of clot formation; ZENAPAX® (daclizumab) (Roche Pharmaceuticals, Switzerland) which is an immunosuppressive, humanized anti-CD25 monoclonal antibody for the prevention of acute renal allograft rejection; PANOREX® which is a murine anti-17-IA cell surface antigen IgG2a antibody (Glaxo Wellcome/Centocor); BEC2 which is a murine anti-idiotype (GD3 epitope) IgG antibody (ImClone System); IMC-C225 which is a chimeric anti-EGFR IgG antibody (ImClone System); VITAXIN® which is a humanized anti-αVβ3 integrin antibody (Applied Molecular Evolution/MedImmune); Campath 1H/LDP-03 which is a humanized anti CD52 IgG1 antibody (Leukosite); Smart M195 which is a humanized anti-CD33 IgG antibody (Protein Design Lab/Kanebo); RITUXAN® which is a chimeric anti-CD20 IgG1 antibody (IDEC Pharm/Genentech, Roche/Zettyaku); LYMPHOCIDE® which is a humanized anti-CD22 IgG antibody (Immunomedics); ICM3 is a humanized anti-ICAM3 antibody (ICOS Pharm); IDEC-114 is a primatied anti-CD80 antibody (IDEC Pharm/Mitsubishi); ZEVALIN® is a radiolabelled murine anti-CD20 antibody (IDEC/Schering AG); IDEC-131 is a humanized anti-CD40L antibody (IDEC/Eisai); IDEC-151 is a primatized anti-CD4 antibody (IDEC); IDEC-152 is a primatized anti-CD23 antibody (IDEC/Seikagaku); SMART anti-CD3 is a humanized anti-CD3 IgG (Protein Design Lab); 5G1.1 is a humanized anti-complement factor 5 (CS) antibody (Alexion Pharm); D2E7 is a humanized anti-TNF-α antibody (CATIBASF); CDP870 is a humanized anti-TNF-α Fab fragment (Celltech); IDEC-151 is a primatized anti-CD4 IgG1 antibody (IDEC Pharm/SmithKline Beecham); MDX-CD4 is a human anti-CD4 IgG antibody (Medarex/Eisai/Genmab); CDP571 is a humanized anti-TNF-α IgG4 antibody (Celitech); LDP-02 is a humanized anti-α4β7 antibody (LeukoSite/Genentech); OrthoClone OKT4A is a humanized anti-CD4 IgG antibody (Ortho Biotech); ANTOVA® is a humanized anti-CD40L IgG antibody (Biogen); ANTEGREN® is a humanized anti-VLA-4 IgG antibody (Elan); and CAT-152 is a human anti-TGF-β2 antibody (Cambridge Ab Tech).
Production of Exogenous Protein by Transgenic Avians
[0138]Methods for the production of heterologous protein by the avian oviduct and the production of eggs which contain heterologous protein involve providing a suitable vector and introducing the vector into embryonic blastodermal cells so that the vector can integrate into the avian genome. A subsequent step involves deriving a mature transgenic avian from the transgenic blastodermal cells produced in the previous steps. Deriving a mature transgenic avian from the blastodermal cells optionally involves transferring the transgenic blastodermal cells to an embryo and allowing that embryo to develop fully, so that the cells become incorporated into the bird as the embryo is allowed to develop. Another alternative is to transfer the transfected nucleus to an enucleated recipient cell which may then develop into a zygote and ultimately an adult bird. The resulting chick is then grown to maturity.
[0139]In an alternative embodiment, the cells of a blastodermal embryo are transfected or transduced with the vector directly within the embryo. It is contemplated, for example, that the recombinant nucleic acid molecules of the present invention may also be introduced into a blastodermal embryo by direct microinjection of the DNA into a Stage X or earlier embryo that had been removed from the oviduct. The egg is then returned to the bird for shell development and laying. The resulting embryo is allowed to develop and the chick allowed to mature.
[0140]In either case, the transgenic bird so produced from the transgenic blastodermal cells is known as a "founder" Some founders can be chimeric or mosaic birds if, for example, microinjection does not deliver nucleic acid molecules to all of the blastodermal cells of an embryo. Some founders will carry the transgene in the tubular gland cells in the magnum of their oviducts and will express the exogenous protein encoded by the transgene in their oviducts. If the exogenous protein contains the appropriate signal sequences, it will be secreted into the lumen of the oviduct and onto the yolk of an egg.
[0141]Some founders are germ-line founders. A germ-line founder is a founder that carries the transgene in genetic material of its germ-line tissue, and may also carry the transgene in oviduct magnum tubular gland cells that express the exogenous protein. Therefore, in accordance with the invention, the transgenic bird will have tubular gland cells expressing the exogenous protein and the offspring of the transgenic bird will also have oviduct magnum tubular gland cells that express the exogenous protein. (Alternatively, the offspring express a phenotype determined by expression of the exogenous gene in a specific tissue of the avian.)
[0142]The invention can be used to express, in large yields and at low cost, a wide range of desired proteins including those used as human and animal pharmaceuticals, diagnostics, and livestock feed additives. Proteins such as growth hormones, cytokines, structural proteins and enzymes including human growth hormone, interferon, lysozyme, and β-casein are examples of proteins which are desirably expressed in the oviduct and deposited in eggs according to the invention. Other possible proteins to be produced include, but are not limited to, albumin, α-1 antitrypsin, antithrombin III, collagen, factors VIII, IX, X (and the like), fibrinogen, hyaluronic acid, insulin, lactoferrin, protein C, erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), tissue-type plasminogen activator (tPA), feed additive enzymes, somatotropin, and chymotrypsin. Immunoglobulins and genetically engineered antibodies, including immunotoxins which bind to surface antigens on human tumor cells and destroy them, can also be expressed for use as pharmaceuticals or diagnostics.
[0143]One aspect of the present invention, therefore, concerns transgenic birds, such as chickens, comprising a recombinant nucleic acid molecule of the present invention and which preferably (though optionally) express a heterologous gene in one or more cells in the animal.
[0144]Suitable methods for the generation of transgenic avians having heterologous DNA incorporated therein are described, for example, in WO 99/19472 to Ivarie et al.; WO 00/11151 to Ivarie et al.; and WO 00/56932 to Harvey et al., all of which are incorporated herein by reference in their entirety.
[0145]In various embodiments of the transgenic bird of the present invention, the expression of the transgene may be restricted to specific subsets of cells, tissues or developmental stages utilizing, for example, trans-acting factors acting on the ovalbumin transcriptional regulatory region of the present invention and which control gene expression in the desired pattern. Tissue-specific regulatory sequences and conditional regulatory sequences can be used to control expression of the transgene in certain spatial patterns. Moreover, temporal patterns of expression can be provided by, for example, conditional recombination systems or prokaryotic transcriptional regulatory sequences. The inclusion of a 5' MAR, and optionally a 3' MAR region, in the novel nucleic acid molecules of the present invention will allow the heterologous expression unit to escape all, or in part, the chromosomal positional effect and therefore be expressed at a more uniform level in transgenic tissues that received the transgene by a route other than through germ line cells.
[0146]In various embodiments of the present invention the transgenic avians comprise a recombinant nucleic acid comprising SEQ ID NO: 1, a truncated variant of SEQ ID NO: 1, or the complement thereof.
[0147]In one embodiment of the present invention, the transgenic avian is selected from the group consisting of a chicken, a turkey, a duck, a goose, a quail, a pheasant, a ratite, an ornamental bird or a feral bird. In a preferred embodiment, the avian is a chicken.
[0148]In various embodiments, the transgenic avian produces the heterologous polypeptide in the serum or an egg white, or both.
[0149]The present invention is further illustrated by the following examples, which are provided by way of illustration and should not be construed as limiting. The contents of all references, published patents and patents cited throughout the present application are hereby incorporated by reference in their entireties.
[0150]It will be apparent to those skilled in the art that various modifications, combinations, additions, deletions and variations can be made in the present invention without departing from the scope or spirit of the invention. For instance, features illustrated or described as part of one embodiment can be used in another embodiment to yield a still further embodiment. It is intended that the present invention covers such modifications, combinations, additions, deletions and variations as come within the scope of the appended claims and their equivalents.
Example 1
Construction of a Complete Ovalbumin Locus from Two Overlapping BACs
[0151]A complete ovalbumin locus BAC was created from two overlapping BACs that together contained the complete ovalbumin locus, as shown in FIG. 6. The nucleotide sequences of BAC 120 and BAC 77 are in opposite directions with respect to the vector backbone pECBAC1.
[0152]BAC 120 was digested with Not I and a 145 kb fragment was re-cloned, but in the reversed orientation (flipped), into Not I digested vector backbone pECBAC1. This resulted in a deletion of a region of approximately 11.5 kb from the 5' end of the insert sequence of BAC 120 and which was upstream of the DNase I sensitivity region. The reversed BAC 120 `flip` and BAC 77 clones were digested with Srf I and RARE digested using an oligonucleotide targeted to an EcoRI site within ovalbumin. 5' and 3' fragments were isolated by CHEF gel electrophoresis, and ligated together to yield the complete contiguous ovalbumin genomic locus BAC.
Example 2
Expression of a Heterologous Gene by a Chicken Ovalbumin Locus BAC
[0153]cDNA constructs encoding immunoglobulin light-chain and heavy-chains of a human IgG1 kappa monoclonal antibody were inserted in-frame with the ovalbumin translation start site of separate ovalbumin locus-containing BACs, as shown in FIG. 3. The immunoglobulin chain-encoding cDNAs were first inserted into a plasmid that contained a 2.7 kb EcoRI fragment from the ovalbumin gene and which included the ovalbumin start site. The resulting vector was then digested with restriction endonuclease EcoR1 and cloned into an approximately 195 kb ovalbumin BAC which had been subjected to EcoR1 recA-assisted restriction endonuclease (RARE) digestion as described by Boren et al., 1996, Prot. Sci. 5,: 2479-2484 and incorporated herein by reference in its entirety.
[0154]Transgenic birds were created by cytoplasmic co-microinjection of human light-chain and heavy chain BACs followed by ovum transfer as described in U.S. patent application Ser. No. 10/251,364 incorporated herein by reference in its entirety.
[0155]A hen carrying these constructs was grown to sexual maturity. Eggs were collected and the egg white material was assayed for the expressed human monoclonal antibody using sandwich ELISA as described by Harlow et al., Antibodies: a Laboratory Manual. 1988, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory. Xiii incorporated herein by reference in its entirety. The human monoclonal antibody was captured by a goat anti-human kappa chain specific monoclonal antibody and quantified with an alkaline phosphatase conjugated goat anti-human gamma detection antibody. Hen # AA698 expressed up to 1025 pg of human monoclonal antibody per ml of egg white.
Example 3
Expression of a Heterologous Gene by a Chicken Ovalbumin Locus BAC
[0156]The open reading frame of the firefly luciferase gene was inserted into the ovalbumin translation start site of an ovalbumin locus BAC as shown in FIG. 3. The luciferase gene was inserted into a plasmid that contained a 2.7 kb EcoR1 fragment from the ovalbumin gene and which includes the ovalbumin start site. The resulting vector was then digested with EcoR1 and cloned into an approximately 195 kb ovalbumin BAC which had been subjected to EcoR1 recA-assisted restriction endonuclease (RARE) digestion as described by Boren et al., 1996, Prot. Sci. 5,: 2479-2484 and incorporated herein by reference in its entirety.
[0157]Primary tubular gland cells isolated from the oviduct of laying quail (Sanders and McKnight, Endocrinology 116, 398-405 (1985)), were transfected using the ovalbumin-luciferase construct or with a negative control CMV-IFN construct. Luciferase activity in cell extracts was analyzed two days post transfection (Table 1).
TABLE-US-00001 TABLE 1 DNA RLU CMV-IFN 60 Ovalbumin Luciferase 274
Example 4
Basic Local Alignment Search Tool (BLAST) Analysis of the Complete Ovalbumin Promoter Sequence (SEQ ID NO: 1)
[0158]The complete approximately 195 kb ovalbumin promoter sequence (SEQ ID NO: 1) was submitted to the National Center for Biotechnology Information for BLAST alignments with database sequences. Further analysis was by using the GenScan and MARWIZ software. Percent identities between the ovalbumin gene region sequence (SEQ ID NO: 1) and corresponding known ovalbumin promoter features are listed in Table 2 below.
TABLE-US-00002 TABLE 2 Nucleotide positions of identifiable elements in the region of the chicken genomic within BACs 120, 77 and 26 Nucleotide Positionsa Domain Identity 5963-1 Qb 9730-9922 CR1 10772-11935 CpG Island 18914-19088 CR1-GG 20106-20921 CR1-GG 39975-24820 R ATPase 41119-41177 CR1-GG 41586-41700 CR1-GG 41701-41800 MAR element 42221-42742 CpG Island 43505-46990 S Gene 50017-51427 T Gene 56001-56201 MAR-like element 56501-56901 MAR-like element 64599-71919 U Serpin Gene 58401-58701 MAR-like element 74883-75634 CR1-GG 75420-75634 CR1b 76251-76451 MAR-like element 80151-80451 MAR-like element 81125-94938 V Serpin Gene 81832-82120 CR1 85473-85922 CR1-GG 88654-88797 CR1-GG 90120-90167 CR1-GG 96401-96800 MAR element 97884-97965 Y: OV-1 element 99080-99107 SDRE element 100602-107839 X Gene 110247-111200 CR1-GG 114779-121099 Y Gene 117849-118132 CR1-GG 131729-139290 Ovalbumin 144651-144850 MAR element 147721-155849- W Gene 150801-151600 MAR element 156581-157181 MAR-like element 157081-157581 MAR-like element 157132-157331 MAR-like element 159095-165114 MENT 163701-164100 MAR element 171633-180432 Z1 183204-190418 Z2 186201-186590 MAR element 190101-190800 MAR element 192078-195101 Z3 aNucleotide positions of protein encoding regions are from the beginning of the first exon to the end of the poyadenylation signal-exons are shown in FIG. 1 bProtein coding regions are given in bold
[0159]Although preferred embodiments of the invention have been described using specific terms, devices, and methods, such description is for illustrative purposes only. The words used are words of description rather than of limitation. It is to be understood that changes and variations may be made by those of ordinary skill in the art without departing from the spirit or the scope of the present invention, which is set forth in the following claims. In addition, it should be understood that aspects of the various embodiments may be interchanged both in whole or in part.
Sequence CWU
1
31195102DNAGallus gallus 1tgcaagcttg caagttttac tgtctcatat ggttcacata
ccattcattt agcgtggcct 60cacaaccaac ttgggaacca ctgccctaag taacaatacc
tcacatatcc ataggatctt 120caacaccatt ctgcttaaaa ttcaaatccc tttcattaaa
atagaagata tttagataca 180agataaatag agcatatgtt tgtatgtggt catcttcagt
tctagaaaca gtttcttctc 240tcttatgcaa ctgaaagtag cttagtttcc acagccccgt
gcagagcaat acggttgtac 300tgctctcaaa gaagtaccaa cacaacatgc atcagcagag
ttgcacaaga ctactgaaat 360aaaatttgct tagctgctag tgaaaacata agtatccttg
cttgtaaaca aaagcataag 420tcatttcaca cacctccatt tgcagggctt cagctaatcc
tcgaagagca aacttggtcg 480gagaataggc tgtataacca aaaaggccta attgcccagc
ctgagatgat acaaacacaa 540tccttcccat ccgtcgttcc ttcatggtag agatgactgc
acgactcggg taaacactac 600ctagataatt gactgccatt aatctctgtg ggaaaaaaaa
aagatattaa aatggtattt 660cagtcactga ttcagaattc ttcagatgtt tagttttatt
tctccaaaat ccctactgag 720gcttgcttta acatcaaact ccactacatt tctatcagcg
agcaaagtaa acaattgttt 780tctactgtat ttgtttcata aacgttccat agcaaagaat
ctcaccgtaa gacaaaaaga 840aatgcactgt atatacaagt gttaatggca ttgaggatac
taatgatgtg agctattatg 900gctttcacaa agaaaaaagg gcaaattaca aaaaaaactg
acttgaacag tgtctaggaa 960gcaatccatt tatacaagaa gtacaaaaac attgaagtga
aagtttaaag ggcttcctaa 1020aaaaaagaaa gaggaaaaat tctatgtata tatgcacaag
ctctcatctt ggcaggtaag 1080attaattcct gtttcaatct cctgaagacc taaaacacaa
ctggtggcct acacaagcaa 1140taaaacagat tatttaccct tactgttaca cttactataa
cattatgcct attcagtatc 1200acgtgcattt tcagatggct ctgtgatgaa gtgagaatca
aagtaaccag aaagtagaga 1260ggctgtttgg ttttttttaa tgtattttgg tttgggtttt
actgttgttc ctttctttcc 1320accaagctgc accaagttac caacagttta cataccgcag
agttgaatgc tgacaagatg 1380gattcttctt taacagattc aaaatatggg aaaaagaccc
acacatcttc accaggaaaa 1440aagagcccaa ttcagtgtct ttcacagcgt gttaaataaa
agcacacatc aaccttcctt 1500tccatcttga ggtccttccc tatcccaggt ggggaattca
ctgttctgaa ttgtccatgg 1560accaataatc aggtaaaaaa ataagttgca atccttgcaa
atctaaaaat gcatacaaag 1620accaccacta acgaaatata ttgctactca cacagtccgg
aaagacacag taaaataatc 1680tattccttag aacagcggaa tgacaaatta cttgcaatga
aataaagcat gatcactcac 1740ttcaaaagaa tttacttcaa tatcctcaaa ttttcctgta
actgatgttc ctgcacagtt 1800gacaagcagg tcaactggtc ccagcttctc ctgagcctgc
aagaggacag aaattaagct 1860ctccagcagg aaaaaaagca ttcatatagc atagtgtcat
cattaaatat actgccactt 1920gaaacacatc gtacagagat gaccttaata catacgctgg
tggaacagtt gcttctcacc 1980tgttttagaa cgttctccac ctgttcatag tctttagata
catcaacaga aatacacagc 2040acaacctaaa aatggaaaat gaaagaacat attataacct
cagtccaaga atggttggcc 2100ccaagtctta caaaagaaac tgcacactgc ttcatgaggt
catattaggg acaaaattag 2160aaaaaaacaa gactaaaaaa aactgattag tggcacagag
ctaaatatgc ctaaaaagtg 2220atttaacgtt tactctttgt ctgggaacaa gaggttatgg
atcttagttt gaacgataca 2280ggaaaatgaa aatagacact ggcccagggg agaataaata
aataaatagt aaaaaaaaga 2340gagacagcaa tgtcttaatt tttgttgcag ctggaacgta
ggtattgatt ttctttaaaa 2400ctgctgatta catttagcaa cttgatctca gaatctaagt
tttgaagcac cttccagtca 2460acttccagcc aaaggaagca ctgactttgg ggtatcacca
actgtaagat cactgccaac 2520tccagcttgg ccggggattt acaaagagat tatcccctct
gctaaacaac tatcaaggtt 2580ctgaggcaac tcagtatctt gaaaggagaa gcaatcacat
accacaatag aagtagagac 2640tcctgtattc tcattctgat ttctacgtct tactttgtcc
aattccctta aagctggttt 2700gaggtgaaat aaagtcatca ccggattcta acaagcgtca
tcagggtcac ttgtcatgaa 2760gcacgccaac aaaaatgaag aaaagcttac caaagtgtaa
ctaactgctt tggcagctag 2820tcatttgtca gttttgcctc tggtcagact gcaacacttc
ccagctacat aaacagaatc 2880tgcttgccat tcctgtgcag caggttggct attcctgcct
tccagcactg tgcatagctg 2940gacatcggag taaacttgtg gtgcaagtta atggttggcc
agcaagcatc tccccttagg 3000gcatttttag attttagaaa tatttatctt ttacataagt
ttgagaacaa aaataacagc 3060tgagctaaga taacactgat ttactatctg gatgtcttta
caagatcagc aaggttaaaa 3120atcacagttc catacgaaaa ttggacatcg gaccacagat
cagttaatca tactgagaac 3180aatatactca ggaactacta aagcttgtat tggctaccag
cacattatac aattcatctt 3240tttgcttcta ttattgtatt tcctttctgc tacattaagt
tgattacctc cagtccagag 3300catgcactgt gaatgtggtc ctaataaaca gactatgctg
ccaggaagtc taatatcctc 3360attccagttt ctcgtctttt gttctaatag cgcattatct
gaccattcct aaagcattcg 3420tttctcaata aaagctcaac tccactccca gtgaagtaac
taggaatatt ccatactgag 3480aaagtgattt taaccatttt ccaaaaaaat tatgggcaag
catttcattt cactgcatcc 3540ctttatttgt aaaggctgca ctgtcagcat tctaaataaa
tcacatacaa atgtatttca 3600gagaacacgt gccaaatcca acacctaaag gtacgttgac
accagaatcc tggttctcaa 3660tgcaacttag tttgacatat atatacatga gcagaatgca
cagcatataa tcatgtaaaa 3720actggtattt cctctggatt tttgctccct agcttggcat
cttgcaaatg taacagcctc 3780tgtggtctgg caacccatcc cagacaactc tatactttca
gcaatcttct tgtacagcct 3840ctgtctcttt cgctgccctc atttgtcagc ttcttatctg
ccgttcttct tctatttgtg 3900ttctttgaat ttagaacagg cagacttccc tgtgacaatg
actacaactc aacagtaggt 3960acacgagggc actcccacta cagccagatg aagtatggac
aagtctaaat ttgctactgc 4020taaaacaatc ctgtttagct cacactgaag cttgttcagt
cttgaactta ctgaaatttt 4080aactgaaact cttgcagaca catacctagt actcttctta
agaaaacaga aatgtaagtc 4140cacatctgtc aagcttggac agatttttaa caagagcatt
gtgacagtgt ttgacaaagg 4200atcaaagtca ttgtcaaccc tgggaagaga ctagagcaac
taaagggaaa cccttactgg 4260ggtttatttg tttttaaaag gggacaaaac gttgctcctc
ctcacctcac atgacttcat 4320atttcaagat aaatttgatc tgagtaaggc aacatgcaca
gaaagaaaga tcaccttgaa 4380ccatttgaca ggaatgagca aagacagaat cttgctgagt
gttcttgata tcttcattta 4440catcggctag tactaacatt gcttccaaat atccatcttt
ctccactgct tcttttcagg 4500agaatatcca acttatccat tcaccagatt attatacacc
caatagacac caaatccctt 4560atcacaaaca aattgcttgc acttgtaata aagaaaataa
gaaccaacta ctggagcaca 4620agagagagga aatcaaagat gcgatggtct caatgctgtt
tcccgtctct tttggattat 4680gagtattaag aaaaaaaagt tgtagtattt catcttgaag
ctgtgttttt ttgaacacag 4740aaagctgccc tattttaaaa catagcaccc cagcaagcat
taatgcactc aaacagtgca 4800ttaagcgtga atacattatt tatttaccaa agtacctcca
gtgtttagaa acctcagtgc 4860tttgccagga aaacagcaat acacagcaca acatagggaa
tgaaatctga aaataagtac 4920ctaaaattta ttccccatta caattgctgc atttacgatc
tctcgaagag aaacttgaaa 4980aaagtaggtt attctcctca atcttgaaca atacaaacac
ttatcaagtg acagcaaatt 5040gcagctatca aaaaaaactc taatacacct acaggtgaag
ataccaatct ttttctctgg 5100gaaaaaaagt tagcatacta accaggcaac atcaaatgta
gctgagtttg gaacatcaca 5160cgtagacaga ttggaatttt ttgaattcaa gtgaggcctc
acctattttt aatcaaacag 5220tttggtttta aaaaccagac tactgaagct atgttgaagc
gactaccttg aaacacaaca 5280gttataggta gcagcgatct tacctgcttg tcattaacag
agtacttttc tatttccttc 5340tttgtctgta acagcttgtt ctgaaacgga taataagaac
agctaaagca ccgcagttat 5400gagaacacat ttgtcttcag tatttcgaga agccctagaa
acactctcta aaaacaagaa 5460cattatcatc agctagcgat aacgctttac tatgttcatc
ttagaggttt atgaacgtac 5520aaaacatcaa ctccatgcca tagcaatgga agagttttaa
ggcaagctca tcacaaactg 5580catgttagat tacactactt acaaaggcag ccatctctac
tctgcaaata catcacattt 5640aaattattag gatcacctta aagcatacag tggaaaaaaa
accaatgagt aattatcaat 5700tcccaaatta ctcctcaacc ataccaattt cattaatata
aggtagccag caaaaataca 5760aatacgctta cctcatctct tgcaatcagt gttatgaaag
ctccttgctt ataacattca 5820atagcaatac attttccaat tccactggag cctccagtaa
cctggaagtt atttgaaaag 5880tcagttagtc cgttattctc atccctaccc ctgtatcata
acaagtcaaa tcaaaactct 5940aaaagtgtac tgagattcaa atatcaccca gtgaagtatg
aacacatgag atatacaaaa 6000gtttctttac ttactgagta cgaatggaaa aatgaaatct
aactaccttg tcaggcaact 6060ctctctgtcc caaaaaccag ctttacctta atggtatttt
ttccatattt cttatgtcag 6120gctcacttag acccactctc acgtgccttt gattaactta
tttttgaagt acggtaatag 6180ttataagttt gcaatcacta ttaacattaa gaacgcaatt
acatcctgtc cctccatgcc 6240aggctacaat aaaatgcata aaaagagcaa tcatagcttg
tctgccaaaa acaccgtgta 6300aacaaacaca atttaaaaag ttatcttctt tcttattaaa
taaacatcct taatttgaca 6360gctggccccc tgcagagcac gtctcacagt tcacatctgg
atgctgaact gcctcaatgc 6420ccttctgtgg ggatcccagt tctaagcctc cataagcctt
tccttcttcc tctaaaccat 6480aactctcagt ggtctcccat gcaaacggga aataaacagt
ttgcctgcag acaaatgcaa 6540ggcgatggct acaacccccc actaataaac aggttaaagt
cacctgaaca cccccctctc 6600taccctccag aaaaatctat ttgtgaactc catttaaaac
aaaacataga tggtgaccca 6660actgctcttc ttcttatcgc aaataaaact gtcatcctga
tactgacttt tggtgatttg 6720ttcatttaaa cccagcctca gtttgtacgt gccttcagtc
cttaaacaca cagagttgta 6780ttttccttgt atggccaaat gtgttgaatt ttctcctaag
agcgtatcac tgagaaaaga 6840ttggaatgtt tcattgatga agggagttct gaaatacagg
cttaaatatt tgagaaagag 6900attcatattc ctctggaagt tgtcatttct gttctttatc
aagacttcag agaaagtaag 6960gtatcacctc tatttcaact atgagaaatg gaatgcactg
agccaatcag tggaagagca 7020aaggcttcca gtctcatctg cacgtgcctt tgttttgttt
tcaggctcac taccctcaaa 7080attctttcat attcctgggg gcagtaaagt caaaagagtt
agaagtacgc tcccatgagt 7140caccaagctc ttctttctta cgagtgtttc atcacaaacc
ttcaaagaaa acagctcagt 7200acacaaaggg tgatcatgta acctcagcaa acttattttc
aacagattcc acagtttttg 7260tctaatttgt ctgctgtcac cagtgggtca agaacttaga
aagactaact tcaacttaaa 7320cagagtttca tagacttccc ctattagaga aagtcgcttc
aaagcagctt ctaatcgact 7380ctttactacg ctaacctttg tgaatgactt taagatgcac
gttctgttcc ggagatgttt 7440aatgctgttg cattctgaat aaaggatggc tgcttcctaa
gcaaattaag tctgtaagaa 7500cgagtgttaa cgtttgggaa ccctagcatc atttaattct
tcacaacaga aagaagttaa 7560atttttagtt cattcccaaa gcacttttga agattccaat
acatctctag aaggattagg 7620agattcagtt tgatgtattc aggcaaacct cagttccaga
gcactcatga gctgccttta 7680cagattttca gcagcagctg gtacatcagc acaggaacta
atgaatgcat gtggaagact 7740gctccaattc ccatttttgg cacagcactg aggcacagct
gattgctacc tgtacaaatt 7800gctaagtgtc atgaacaggt caactacagc agtgtttgaa
ggtgaaggtg gcataatgcc 7860tatggcttgt tagacaacgt taacgttcac ttcggaaaga
caaggtctga agtcaagcaa 7920gaaagggaag tttttatttg cagtcatacc caagaacgct
agatagggct catgtcagat 7980tggtcagtga caaaaagcag aatcagttag ctaggaactt
cagcatttag gtggaaggct 8040gttactaata cgttggaggt tgttactaac atgcaccacg
agatgcaatg taacagtaaa 8100gaaacagaac aactcatcga tcaatgagta agcacaattc
caaatcatca caactatcat 8160cagaagcgat caaaatgaag acttgatatg ctctttccaa
cagggcttgc tagctttcac 8220tgagcagcag cacaggatgc aagctggctt cttgctttta
gacagtactc taacagtagg 8280cattcagttc cactgaaaca gaacatcccc gtaacttgct
ccaattggct ggtgtgcctg 8340actggactcc aaatgacctc ttcctgaacc acccatgtgc
atttaagcca caggtcacgc 8400tgctcccaat gcaagagcta caaagctgag acacggagat
gggtcaactc caggaacacc 8460caaagcctgc tgtccttatc tgatgggctc tgcagggaaa
tgctgaaact actcctagtc 8520ctgagatggc ccttacacct gtggcttggg catactgaag
cttaactatg tccaagttaa 8580aggccctgct caaactgaaa ggaagacaat cacaggcagc
aaatatcctt acattcctgg 8640catagttagc ctatgaaagc aaaaatacat ttaaaagcat
tttgttttac taaataagca 8700aaaactgatg cttgagtcaa cctgatagct gtgaagtcct
tcctatttaa cagtgcccac 8760tgagattacc aaaacacaca gcagatatgg aacaggaaaa
cacccccaca agattagatc 8820agtaatttgg agttctgtgg agctccattc ctctctaagc
agaagagaca cctgtggtct 8880cggtcccact ctaaagcata cctcatgtca cacagcaagt
gactccagga gaggacagga 8940gggaaaaaaa ccccaacaaa aaaacaacca cacattttct
gcctcagatt tctgtccttg 9000tacctcaaac acctagctct attaaccatt acaactgtta
ggagcagaga gaaatagatt 9060ctacaaatga ttaagtaggg tgtgaaaaaa tgctgattta
aattgagaat tcaacaagta 9120actgctagga aacaatatac acagtagcgt ccaaacacat
tcaatgaccc tctttaagaa 9180atactacagg tattctcctg cacatatgaa gggggaatta
tacatacaga tcacacaaac 9240agattttgac tttttctgtg aaaacacttc aaggaagttc
ttaacagtgc tacggtcctg 9300aatattttca tgacaccttc tgataatttc agtagcagag
aacactcact cccattatac 9360gatgcaagaa ttcaccttgc acatgtgatg tgtacagcct
tcacaagtac agtttctaat 9420ttacaaaact atcatccaat gtttttcctt acacaattca
cccaaacatt tgaaacccaa 9480acatgcaagt ctaacagcat cagggttatt agcagcacct
acatatcaaa aaaactaaaa 9540ctctgaaaca actgctccat gtttcatgtg tttgcacaaa
tgctttcaga gcataactga 9600agccatagtc aaacaagtgc ttctgccaga agcaatgcca
ctgaagctga tcacccacag 9660atttgtaccc tgatgccaat aaatacctac agcctttcct
tctattggga tatgaacaca 9720gttaatcctc cagaaggtgg tgacgcactg gaacaggttg
cccaaggaag ttgtagatgc 9780cccatccctg gaggcattca aggccaggct ggatgtggct
ctgggcagac tggcctactg 9840gttggcgacc ctgcacacag caggggggtt gaaactagat
gctcattgtg gtccatttca 9900acccaggcca ttctataatc ctacgaatag tcattttgag
accatcactt atgtcaaatt 9960caggttacgt ggctaataca attagcagta gtggctgtga
gggaagattt ctccaacaag 10020attattcttt gtcattttca ttgtgagcca actgaagtgg
ctctttgaaa aaagaagaac 10080cagcagagta gctttggaaa aagcgtaacg acacaaagaa
aagacaacac tcgggataat 10140cagattaaaa acaaacaggt ggacaatact ctgggataga
atacactgaa cattttgttg 10200cttactattc tgtttccacg caagcactgc agtaccctta
ccctgcttca cctttgcttt 10260tacacagtac agaaggattc ctgcttagca agagttaccg
ctgtgggaag aacctcagag 10320agccttcact cacgctctac tatctccagc aggacatgat
gctgtaaagc cagttacaat 10380acccagcaat acctattgca tcaagtaatt tgggaacact
gttgcaactt ggacagctcc 10440aagccgggac agctctatcc gcaaagagca gccctaaaac
aaataggcag ataaaaatga 10500acacaaacaa acaaggcacc acacagagct caaaaaaatc
ccaaatgcca agcagctgtc 10560aattccctcg cacctcagag gtctaacttc tgcattacac
ccaagtcctg tagtaaccaa 10620gtcctgtagg cagcctgcat gccctactca ccccccaaaa
gcagattcag caagcaaaca 10680gcacagctcc tctacacgga gcacaccacg ggtaactaca
gctaagtccc cagagctgac 10740tgaaggacca cagccgcccc gacccgctct cacgcaccca
catgctccac acacacctgc 10800aggcccctgg ctgctgctcc ccatcacgca cccgcccagc
tttcagcggt acgctcctgg 10860ggaaccgttc aaaagctata ttttcccgaa taaaccctcc
caaaggctcg ctcctacaca 10920gctgattaca gacaagccaa acgtcgctcg tggacacgga
tacccgcgct gagtgccgcc 10980tgaccgcttc ccttcgcgtc agcccgcccg tttcctcagc
acgggtcgcc tttcagcccg 11040tgccttccac cctcgtgagg agggccgcac tccagcacct
cggcagatgc agcggggcct 11100tccccgggac acggcggccg cggcctcccc gctccctcct
cccgcccagc gccggcagcg 11160gaccgctccc ccgcggtcgg tcagccagca gcggccggga
tcggggtggg gagggggagg 11220cgagaggcct cgttcgactc accacgacgt gtgcgccggg
cagcttgagg ggcttggggc 11280tgatgagcgg cgacaccatg tagaggagaa ggacgatggc
cacgatgaag gcggccgcca 11340ggagcagcat ggctcgggcc gggcttgccg ctggggaggg
ggcggcgggc ggtgacaagg 11400cccctggggc tgcgggaggc gccgagcgcg gcgcggcccg
gcgcggcacg gacagcggga 11460gtagaaccgg tgcccgcctg cgccgcggcg ccacggggcc
acaggggagg gggaggagga 11520agaggaggag ggaggaggag aacacggccg ccactccgcg
ccctgattgg ctggtgggcg 11580gggcgggcgc ggcctcgcga gcggggattg gccagtgagc
gacgggaagg agctggcgga 11640ttggccgaga ggcgggacgg cgctcggaga ttggagccgc
aggctgtttg cagggtcacc 11700gttggaggca aagggcggcg gagaagagag agttcctccg
gagaagacgg ggtgcgggac 11760ggcgccccct acggtccccg cctagggcgg gtgaggcgag
gagtgaggta ggccccgccc 11820cttctcgtca cgtggggctt cccgccgaaa ggagggggcg
tggctccgtg aggtgagaga 11880cgggaggctg cgggcggcgt tcacgctctg gaacgcgagg
gcagctgttt gtggggaaaa 11940aaatgtgtag aagcgtggtt tcaaagcata ataataaaag
attaactaaa acgaaaacgt 12000cttgcagctc aaataaaatg attccgtgcc tacgttcaat
atttccttcg ctgtttatga 12060ctgaaaggaa ctccctgaaa tgatttatgt tgtaaacgct
gtgcagcctc tgacttgtag 12120aagggagttt gcagcgtacg cggctttacg gcctcggaga
gattacgatt acgggcgaga 12180gggcgtgcgg aagggtgtga agataatgca ggagatgaga
ttggagcggg gagtaggcgg 12240aaatgggagc agggctgcgg gcagggatta ggcagtcgtc
aaggggagag caaagaatct 12300ggaacaaaag aatccaggaa ttagttctgg aatgggattg
agccgggatc gggcttcggt 12360gacgctttga gagtggtgct gtggggtgga ggtgtgagga
aatgagagga agaaagagcg 12420cgtgctgagg taacagctgc cacggcaagg gtggggagag
agctgacaaa gtggtgtgtc 12480caaggaaagg cggtgtggaa tcgtaggcat ccttaaggct
ggaaagggtc accaagtcca 12540agcaccaagt ccaactagca gaagttggtg taggatatgg
actaggaacg ctgcaagcac 12600agataccgac ttcattcttt gcatacaggg cagtgtatgt
gttatctttt gtagaatatt 12660aattaaacac aaaggaggag attgataatg taatagagcc
tatttatagt tatctagtgc 12720agaatatggc gagacttgaa aagcccaaat gtcagcagca
tggagataaa gcagacggag 12780ataaatccat ctttcacaat gcgatatcgc tttcagaatc
aacatgaggc aagggcatgg 12840ataaaaacaa tcatctgcag ttaatttcta gtaaaatgaa
ggttaaacat gttggtaggg 12900ggcctctaaa aacctcaaat gcatgatatg ctcctgattg
gtcacagtta ggatcacata 12960ttactaaata tttgagaagc ccttgtagat taacgaggaa
tcccctcggt gaattttatg 13020cagaaatcca tactgtcttt tccttttagc taagtggcca
ctttacaacc gtgtgattga 13080caatccaggt agcgtccact cacattttgt tcctggggca
gtgaagtgtc atgaatttat 13140ctccaagaaa aacattcaaa agtgaagacc ttgtgaactg
cttataactc accaatgtat 13200cgccacagca gtaggttttt gactcttttt aggtatgcca
gcaggcactg aagtttgccc 13260tcctgagctg tctgctgtct ggtttgtatt tgtctcatgt
gacctcattc actgaggaag 13320tgcgttcctg acacacggga atggtttgct acgaaactct
tttctcagtg actgtggaac 13380tggaaattga accctaaaaa aaaaaagtgt tgaagccctc
cagtccaaac tttggttgta 13440cataaagcag tatttaatta atctgacctt gattaacaac
atcaaaaagt gtaattttga 13500agcacaaact gaccaaggta tgtatgtacc ttcgggatgg
gtaagaaaat aaaaaggtta 13560acacatgcta attgctttgc taattaatcc ttagaagcag
cttcaacaca acagcgatgt 13620gtttagagaa gaaaatcaaa tacaggtaga ttaaagcgtc
caaactatag gaccagctgt 13680ggttttctgc ttcctcagtt ctgttcatat aatctttcaa
cagacgtttg cagtaacaat 13740gttgtgggtt gagataaatc agtatgaaca aagcatggca
accgaagtaa gaaagtagtc 13800atttaaacac ggaaacaaat gtatgaattg ataatattac
aacacaagtg actgatacta 13860gaggtgtcct tttgatcttc ttgttcccaa agcatacaag
gtacacacag aagagacaca 13920ggctgtgtta agatgccatt aagagaaggc ataaaggttt
gacagagcag gtagtgaggt 13980tgcagcctgg acagactttc ttattgcact tgagtactca
tctgctggat tttctggttg 14040tgtcatattc acgttaggga gagaggaggg aaaaagagca
ggatgcgtag gctactcagt 14100gattaaacaa aaaaaaaaag ctggaaactt cttcatgtga
tttccatcca gtcagtcctt 14160ctgcttttag agaaagcagc atgaaggaaa aacttcagta
gccaaggaga acaacttttt 14220ccttctgttt tcctgaatta acttactttc ctctccaacc
ttctcccttt tgtgtagcaa 14280gcataggtgt tctatgctca tttcttaaga ggtctgttgc
agtaatcatc ataagacatc 14340aaaggcatgt tggcagttct tggattcctg caaagcttca
agatttagaa tgatggcagt 14400ctaggtgagt tgttcctggt caacaagctg tcttgatccc
gtgtcccaaa tgagaagagc 14460taatagggac ataagaactg aaatcagaaa aggatttaca
taacatgctg gcagtagagg 14520agaattgggc aagaaataat gatctgcaca tggtagtgac
taaagcagtg tgactgaaat 14580acttatcaca cccagctgct tgccttgctg ttcttcccca
aacaaacaag caaatccctt 14640gtagctgaac aatagcttct ttactggtcc atcacgctgg
agagatcatc agctacccca 14700tgcatagcag ggtgaaacag ctcccagagc actgtgcagg
tcaaagtact atatgtaccc 14760tgtctgctgg agtgctatca cggtgatctt ctgggtattc
ctagaaggag atttcctgta 14820ctcccaagct caacgtatca tccagaaagt gctcgcctgc
agcagggacg ggttctggcg 14880atctctgcag cttccagcta tgccgcatgc ccttatcgca
atgaactcag gctgggctga 14940tggcccaggt gctggaggct gccagcacgc aggcaggagg
tggttatagc agctcaggct 15000caggtcaaac caaggcttct tgctggggca gaggggactg
actctgtggt gcaaaagcag 15060gtagtatata tatatgtata tatatacaaa gcccagctac
cagctgagag tcccaaggct 15120gctgcagtag ttttgcaatg agcacacagg aaacaagaag
atcgctgaga acactgctga 15180aatcagattt ctgtcttcac acaggtcaag ctgatttaac
tgtttaatgt aattgctgca 15240gttgcttgga aaaaaaaaga aatagtaaaa ccatgtccaa
aatgaaccat tcataactgg 15300tggcccatta tgtgtcacag ccgatgttgt gctgaataaa
taactgtaca ggtattttat 15360atattgagca acatatttat tgaaacaaaa ataatttacc
tcaaaccagc ggtaaaagga 15420agtctttact gtctaattta aataggcata agttaaactc
gggactgaga tgatcttgaa 15480tttcatttgg tgcccatggt tctttttatg tggtacacct
gcttacactt accatcacac 15540tggagcagtt tgcttttgcc acccgaatgt cagacactgc
tatagattta cagtagcttg 15600ggggggctgc aggttggaag agggggttga ggcctcatca
agtgccatgg caaaacaccc 15660tcaagtaagc acggctggaa gcaggaagga tgagggaatg
agctgccatt tcctttgcgc 15720tggaaggatc actgctaaaa cttgtaaata tctgttagaa
acaaacaggg acgttcactt 15780tgtcctgtga tgcaagagca cccattctga atttttatct
cctgcaaagt tgtatttaag 15840ctgatgttta ccgtggacgt tcgtgttaca agatagcctt
tgatactatc aataacaagt 15900cctctttgat gaagtaaagc tacagagtca caaagcatgc
acttgtctga ccctttgcct 15960ggctgcctgt ccaaccacgt tgcaccacta cacccagccc
cacgagacct gctccagggc 16020caagggaatt gagcacttaa gggaaagtgc tttgtacaaa
acatggcgct tatgagtttg 16080aaaacgtaga tccaccaaaa cctcctcagg cacgatgagt
atattttttc tccactactt 16140acagcgctgt gaattctagt taagggcgtt ttgattccta
aagaattttt ccttctaatc 16200atagacgtac tccagtcctt attccagaag gcttactcct
tgtattttga aggtcttatc 16260ctgaaattgg gatgcagagc cattctgaaa atgacagtat
tttaagactt tgctgcactt 16320actctggctt cccacatacc ttcctcttgc aaccttccac
ctcccagaac tgcagcccag 16380cctatcctcc tctgccagaa aatcggatcc cacaggccct
atctcacacc tcccggttcc 16440ccatcctcat ggcagctgcc ctctttccca aggcactcta
tggagcagca gaactgctga 16500gtgcacaggg caaagatctg ccgttccgag agagcagaga
agcatcgctc gggaatcact 16560gcactgctgc agcactattg tattctgcct ttattcagag
gcagtccttc acctatgaat 16620atcactacta ccttactgaa tatatatttt caggaatatt
ttcacttttt agccagatag 16680gaagcggatt ttgtaattac ccttccagca acttacagcc
aattactgtc tctcctcctg 16740attcctgtcc agcaatttgg ttgcagttat tgcttctcca
gagcgggcag aattttttgc 16800tttaggaaat gtacacctcg aggtaatctt tgaagagtga
caggttctaa agttcacaag 16860tttgatctgc tttgggatta agctacctgc taaactacca
cacgccatcc agtcaagcca 16920tttctattat gtgcgtatgg ctgattctta tcacaaaaga
tcaagttaat gatttgcagt 16980cttcggcaag cctctggttt ctttgaactt gctttttgta
agcgatattc tcgggtactt 17040tttgtgcttg tgaagctact gcagtgctct ggagattttc
tttgtgctcc tggctgtcag 17100agttatccat ttctaggcct gcttggccat ccccatagca
cggggagaac cgtactttcc 17160cattgccctt gtacctgcac ttgtaaaaac gctagaggaa
ctgaaattac ttcaagttcg 17220tgccctgtcc tctttcaaag ccattctgag aactttcttt
gcacaacctt tttacaagag 17280ttaaatccgt ttctagttcc aggcaacaca cttgtcatac
acagcgctgg caagggactg 17340ctgtttattt cttgcttgga tgcaattaca cagccatgtg
cccttgtttt cagtccctga 17400tccattatct ttggcattta ctgcaaagaa gctgctgtta
cgcaatggaa atttagatga 17460tctctttttc ttagcttact tctcctctaa cccaagaaat
gagtacagta tagcctgctg 17520aatgcaagga aacctgcacc tgcaaacttt tctccccact
gcgtcactac caaatatgtc 17580agagttgctt gtacttctta agtctgtttc catcccctaa
tggcacgaac cgttgccctc 17640ctgttgtcag actgcaaaaa ggccagcttg tacagatttg
ccctgtaggt ttgaatggaa 17700gaagggaaaa aaatcagaga aactgccagc ttttgttctg
ccgcttgtaa gcttgctttg 17760gtagaaaagt tgaagaaata ggaacatgct ttgaaatagg
attttaaaag gaatcagctt 17820cttatcttcc ctttgggaaa aaatagtgtg aaggacagaa
taaatcagac ggaaaaagaa 17880agaaattgac gtaagagaac tagtcgggca gaaaggagga
ggtggaaaat acccaaaagc 17940agcaggaaag agggaggcac aggttgccaa ttaacacttc
gatcaaagga aaggcccgat 18000caaaaccttt ttcctcctct aagaagcatc accccttccc
actgcttact gcaatgaagc 18060gagcttttag actaagactc aagagaataa ccccaatacc
agtaaagcct gcagaacttg 18120tttttttcat agctgacacc acagacaaac aaacaaataa
ataaatagta gcgcagagca 18180tcagcaccgt ggcagtcatt ccagcaatca cttccccacc
gtgctctcct ataggagagc 18240tgcagcacag gtcagcgtct cccaacccgt gcacttcttc
acggacagat ttgcatcatg 18300cagaccctca gattgcccag gaagaacaga actgcaatgc
ccagaaagag tgtggaagct 18360ctgagaattt atctgcctgc tggacagagc ccatctacac
ctggaacaag cgggcacctc 18420tctgtgctac cagtgctggg taaagaaagc tgtgcagcag
ctcctccctg aacactggct 18480acgttgtgac atcagccctg tggttcctgt ggcagctcct
gcgcttctgc aactacatga 18540gtctagctgg caggccacct gcttgtttcc atatcagcag
cagccacgtg caccatgtgc 18600accatgtgca gggggcctcc aggcaggtaa aaaaaacaaa
caaacaaaaa catctcttaa 18660ttacaggggc agagcagggc tggatacgaa caaacaaaac
cataccaaaa caagcacacg 18720tgtaaagagg aaaaaaaaat aaaataaatc acagctttgc
agtttgtctg tcttcagagc 18780aaatcaaggc tgtgattaat tcgttacaca tcagaactcc
aagcaggctc aagctgagcc 18840gttgcaactg gcattatgaa tggcacactt gaaaaacagc
caggttgctt tccagattca 18900tggaatcata tcatagaatc atagaacggc ctgggttgaa
aaggaccaca atgatcatcg 18960agtttcaacc cccctgctac gtgcagggtc gccaaccacc
agaccaggct gcccagagcc 19020acatccagcc tggccttgaa tgcctccagg gatggggcat
ccacaacctc cttgggcaac 19080ctgttccaag atgttagctt ctctaacatc ttaccacaac
ataataatga aagaatattt 19140aaaaaatccg tgatgggtag gaacttcctg gctgcagcct
gtgctccagc cctcaggtgg 19200tggaaggaaa taatcatttc tagttggaat tttcattttc
tttttttttt cctcagcttt 19260caagtaggca aacaattcac ttgtcttcca gagctcaaat
cactgctgta agtaacagtt 19320ttcatttgtc atttttattt cctctgtgag atggtgatat
ttatagcaac attctcggtc 19380ccttgcttgg atgacttgtg attgctacgg ttcttgtaac
agcattgcca gaacagtagc 19440aaaaggcaac tgctccagca ccggtttttg taagccatta
cctgtagaca ctcatctgcc 19500tacagtagta tgagtcagtg gaaattactg tttatagttt
acagaccaca tgtgacaccg 19560agcatgtttg aaagcaaagt ccctgccttg aatagctgag
atttaaatta gctgaggcag 19620cagaggagga gggagggcaa gcaaaagcag gtcttgccaa
tccatggcat ggtgcctagt 19680gataggtcac caagcaggaa agaaaaccca accctggctt
cattatcaac atcaggccta 19740tgctcaggtg cccgtgactt atttcctgag aagtctcaaa
acacgaccaa cacctgtttg 19800aactcctata agagagctta gcgcctgcta tgatgcaggt
aggatactga tgtttatttt 19860cattactagt gcgtgacaca tccaaagaaa ttagctgtaa
aatgtctagt attcctgcaa 19920aagaacgtaa cagatcctgc acgtggcagg taccatgcac
agatggcacc aacggatgga 19980tgctggcttc ctcacacgtt gagttgttgt ggagttgctc
tgatgaaggg gagcagcatt 20040tgtgagcatt cattcatgga gctggagtct cctaagcaag
gtaacgaatg caaaggtggg 20100agtgttcaag tggcctaggc aggcttgggc agtgagccca
ggtgaacctc atgaagtcca 20160acagaaccaa atgcaagatc tggcctctgc attgaggcag
ctcccactac caatacaagc 20220tgggaaagga ctgagtgcag ccctgctgag gaagacctgg
ggggtattgg tggttgggaa 20280gctggacatg agccagcaat gtaccctcac agcccagaaa
gccgactgta tcctgggctg 20340catcaaaagt agtgtggcca gcacagcaag ggaggtgctc
ctgcccctct actctgtgct 20400ggtgaggcct cacctggagt actgcatcca gatgcggagt
cctcagtaca ggagagacat 20460ggacctgttg gagcgcatcc agaagaggac cacagaaatg
ttctatggaa tgggacacct 20520ctcttacaag aacaggctga gagagctggg gctgttcagc
ctggagaaga gaaggctgtg 20580agttgacctg atagcaacct ggcagtatct aaagggcagc
tacaggaaag aagggaacag 20640actttttaag cagggtctgg tgtgatagga gaaggggaaa
tggtttcaag ctcaaagagg 20700gaagatttaa gttagatata aggataaaat tttttacagt
gaggatggtg aggcactgga 20760acccagcgtt gtggttgaag ccctgacccc tgagactttc
aaggcgaggc tggctcaggc 20820cctgggcacc ctgatctagc tgtggtgtcc ctacgcactg
caggggagtt gaactagatg 20880gccttcagag gtcccttcca actgcaaaga ttctgtgatt
ctagtaaaca gaaagcgtac 20940agaacagtga cctagtcaaa aattgactat cggaagggcg
tgtgggtaga ggtaggcagg 21000caaaactgta attaggtcaa agaaaaatga caggacaagc
ttatctaata tttgggatgt 21060cagtagccaa atgccagtac agaggatgaa cagcaaccat
taagaatttt ttacacaggt 21120aattctgaca acagagaatt tggggagtaa taattgaaat
attattggta aaacggtatt 21180tttaaagaaa aatcaaggtg agagcacaat agctacaaca
tagactaccc gctcaagaat 21240agaaggagca atgttttgat aataataaag tagctgttgg
aaaagcagca aaattggaag 21300caaacagtcc atcaagtgct tgcaataggt tatgtaagtt
gtgtgaatgg ctctaagtca 21360gccatgatta ctaggatgaa tctggttaag acaaacatgt
atggaagcca accatgaaac 21420cacggtcatc attctggagg aaggaagaat tatgcagcaa
aatcaaggca ttcctgcata 21480tttcaataat tcagagctat taaaaagctc cctgtcacga
taatcttcag aaataatgtg 21540aaaaaaatac atagcggagc aaattttcat taggaagaca
actaaataaa cacaaaaagt 21600agatcaaaca atggctcaac agaatattta aagcagtttc
tttgcttcag ctgccaaaga 21660gcaaactacg atcaggtgca gctgactgat aggagcacaa
aagctgattc aagggtatct 21720gcccagacga cgtgtcgaca tgttctgctc cactcattaa
aacaaaagca gtcaactcaa 21780ctctgaaggc tagtagttga ataataataa taaaatcaaa
accaaacaaa ccttaccaat 21840ctctaagaca gacaaaacca gtacttaaac cagggaaggg
acagaactct ggattcagag 21900attaatcagg tgacgtgggc aaagatacag ccagagaatt
taatgagttt ttctaactct 21960atgaaaatat gtgttgagaa aatccactgt tagtcaatgg
gaagaaacat ctgtgaagaa 22020caaagcaagc aagcacagac acaactgatt taaaaactat
tttacccaca gaacataatt 22080tttcatactg cagtcagagg tagcaatagc actagaagtt
aggaaaaaaa cgtaggccaa 22140gtagtcaaag actagtcaca gctggcagca tgaaagatat
gcaagtaatt tatccagtgc 22200ttagaggctg tggttatata aagcaaatat aacctttaat
caactataaa ccaggcagca 22260ttgtttagag tacataggtt gctctgaaag taacgcctcc
tatttatttc cacagaaact 22320acaactgata cgaagagcat aacaacactg atagagcata
ttcccagcta caaaacacta 22380cttttcaact cagtcatcac gattagctct gcatttttgc
cagcgatgag tgagagcctg 22440catgctacgc gcacagaaat ctgcaccagt ggaggtgccc
caccgtcact ggtgctgaaa 22500tgcaccaccc actgcctcac cgcgctcaca tccactgctt
ggtctccata aatattcagc 22560aagcattgat gaatgtcaag aggtgtaatt ttttctgtgt
ggaggaattc agtgacacct 22620ctgcttcatt tgcgcttcca gccaaatgcc attctgtcat
gctgcctctc tgctgccatc 22680tgtcgcacag ccagagcaca taatggaata ctggtaggaa
ggttcaacca ctaccgccat 22740accaccaatg acaccttggg ctgatgatat actaaaataa
atactacctt tggggtagca 22800ctcatagttt aggttaaccc tgaaaactga agtaaatgac
acctccccct acctcccgca 22860gccatttagc tacatcttct gggataactt agctaggaat
ctgtgataat atttcaactt 22920atcctcattg tagaaaaaca gcagaacaaa aggttatcct
tagagctgca gttctaaccg 22980gcagtgttta tttggaatat ttctaaaaac aacttgaaac
accaaacatt aatgtttcca 23040tttccatgag caagtagcga gatgcaagtt taaaacatac
agtatatttt tctacgttaa 23100agataaggga ttacacagtt aggtttaggg aaacccatag
gacaaactga cctacgacaa 23160caacaaagaa acgcatttcc tgagaatttt aagattgcca
taaggactgt catataggac 23220tgttataaag gtcaattaac aagtaattca ggcagtagct
tcaatcctcc aggtgagagc 23280cctgccagtg cgtggctcgc ttctgaagtg ttcaccagag
gcaacagagc aaagaatcct 23340gctgcaacta agatcaagtt tacaaaccac agtaacttgc
atctacactt gaatttcccc 23400cgcttgccca caaaggtcca caaaaagatt tgcagccccc
tgaatcacat tcacattttc 23460cagtgcgaga ccgaagtaaa gctgcaaaac tgaatgactt
tggaaagaac atttcattat 23520gttagcaaac aaaagctcag caccttgcag atcaaagaat
ttgtatttaa gtgttttgtt 23580tagctgtcaa acgtagaaac aaaagtctaa acaaaagtga
tagttttgaa agtaacactg 23640aagaaatact caagaacata actgatgttg tacattttac
ttcatttaag tacagcaaat 23700ttcacccatc ctatgattta tcgagtacgc aaaatatgta
catagaggaa accaaaaccc 23760ataaaaagac aatcatctat gtgcatatgc gcatgtaaca
tatgcacatg aaatgtgcaa 23820ttttctttta atgcaagtta aacaaagcat atgcacaaca
gagttgcaca accattacag 23880aacagagtgt tctgggtatc ctcatgatgt ttcgcatcta
cagccagtgc aaacttacaa 23940ggcacaaact cagtgctgac accgtagtgt tgtaagttca
ggcacatttc aatttgtagt 24000tcttaaagat aataatcaac agaagtgcta cttctgtact
aaagtgccag cctcttccca 24060aagattaagc attaagttga tgtaacctgt acacagtaat
gatcagcggc gttcggattt 24120aacctaacct atcactgcaa ggtctgtggc tatatcgtgc
tatgcgctcc acacctctga 24180gggtatgctg cttcccaaaa tgcctccctc acactcttca
aagactaccc atacctcgcc 24240agccttgacg cgtggactct tacaggttac tactcaatgc
tttttcctaa ccttagccaa 24300acctctgata aaaccagact taaaaaatca gccatcggga
aatctttcgc acacttgcat 24360ttaacaaacc tttgctcaat tgcatagtga catgtgtatc
agctaggaaa gaattaaaaa 24420caaaagcttg ctgcttaagg caaaaatttt taacacagca
cagcagaaaa agccaaatac 24480cgggttcatc agtatttaaa caaagcactg gctcatacag
tcttctcctc acagtgtttt 24540cttccttact ttcacagcaa acacacacag tatgctcaat
tagcaaattt tgttgcattt 24600ctctaaacgg agtgattaac acataggctg actgctactg
aaaacacctg acaaatcgct 24660tctcttgcac cctcaaaaaa gggtttcttt ttgagcctac
cagaagttga aaacccgctt 24720gcgcccaggt ctaatataac agctaaaact gatcatttaa
aaattacaaa tatttaccat 24780gagttgccac atcactctgc taaaattgtg ttttccgtat
tattttccaa tagaagacat 24840ttaatagaca tctgaagaaa acaatacaat ataaaagcgt
aagggtcttt gcaaacagat 24900cttctattcc ttctgcaaag taagaaagga gagagtttat
tggcatttat ttgcagtgcc 24960atcgataaag acacgagaat acttaagaaa gcaaaaagtt
ctagtgatcc acagacatct 25020ttggcttagc cttcctgacc aaagtcttct gtaaacttct
ttaacttctc caggtcctgc 25080tcattaactg ttggctttgt gctagctagc gacctgagca
tatcggcctg tgagaaagaa 25140agcaagaaaa catgcattca gaaaacgtac cgctgctaac
agtattgctg tgaagaaaag 25200taagctttga aaagcctttt aaaacaagtt gactgtagga
actcttattg aaacaaaact 25260tcgagtaagc ctgaacattt ctgcacgtgg accacttttt
aacctcctga cgatagacaa 25320ttagtgagtt tttacaggac ttaagccaca atctgaggtt
cagctttaaa acaattcatc 25380cattcaacaa gtgttatcta ccactgctta ctgcaacaaa
ctgagcttcc catcttacag 25440attcgtattc caattcactt ttaaggacat caggttgaag
tggaaaacca tcacacgttc 25500ccacatattc caatgcccac caacacagaa tacttcatca
ttgatctcca gcaaagtttt 25560actgctcatg actgctaact tctgtttctt cagctcagtc
agttttgtat atttacattt 25620ggctactaga aaatggagtt cagaaaaaaa aaccacagag
gtatgaactc aaattcagca 25680gttaagaaac cttattaaaa aaaaacgtat ataaaaagtc
ctggccaaag gcaaagcgag 25740gagctgctca acacctcacg ttactataaa agcacagggt
taagttaaag tcagcatcat 25800gattttctag gctttctcat ctcatcgtac tacagacatc
ctacttagaa agaattcaag 25860tctgatcttt ttaatgacaa gaactgattc tggactctga
aataagtccc tgtgcaactg 25920tagcacatca gagtctacct tccattagaa gcactgaagg
aattgtattt aattccagga 25980aagactgatg aaaaatccac ttagtttaca caggcagaag
ttttaaggca ggcctgcact 26040tgcttgcatc ttttcatgcc tcctccatgt gcaaatatgc
agatatttct ctcctcaaac 26100tagtgatggt tacatgtgca aagcagtgca ctctacttta
gagggttttt gatccctatg 26160caacacacct tcctttcatt cattacagaa acgtttgcac
acaggaatgg ccatcagcac 26220agatctgata tcgagtcctt ccttcagaca atgcaattac
attcagaacc ttttgctgct 26280tgagggtaaa atatacgagt gctcaatgat ttgtaacctt
ttaaacaatg tatttaaact 26340tcaatttctc tcaaatatga tgttttggtc tgtagacaga
agcaaatatt ttaacatata 26400caaaaaattc cagctgaatg ttagcaagag ctggctgcat
catctgtgat gaagtataat 26460ccaaactacc attgcatcca ccagcttttt acattgcatt
ggttatgctt gcatttcttt 26520tgtgggcaaa atttacctac agcatgttat tcccagttta
cactgaatat aatttcccac 26580ttctcgatgt caataataat gctacagagc aacaggaaag
taacatatcg tggggcaggg 26640attctgaagg ttttaaatga ataaaagaaa aattaaagaa
gggaggaaga ttcaggtgct 26700gtctatactg catgccacta gacaataata aatgcttatc
agggatggag agctggctcg 26760ctgataagca tgttgtattg tcatgctgtg tgttgcgatt
aaaatgtcat ccagtatgtc 26820caagcatgtc taaaaacaaa gggctcagcc aattgccttg
catgctggct ctaaaatgtc 26880ttgagtattt tcagggttct gcaaagcaag aaacaccacc
aaaaaataaa aaaataaaaa 26940caaataccca ccatggaaac tttaggctcc agtaatttat
cccctggaac atccatccat 27000gtcatttctt cagcttcagg atcacctgga gagcaaggag
tgaacaaatc taccatgata 27060tttggattcg tcactgatgg tccttttacc taataaatga
atacataaat aaataaaata 27120aacaaactga agctgaacat ctttagagca aaagcatact
cttaattttc tgtacatgcc 27180ccacccgttt ggagttgtgt agtgaagtgg aattgtgtaa
aggtgctggc atcgttcact 27240ttgaaaacgc acagcagtag tcagatactt gaactcatac
catgtcagaa ccaatgagcc 27300tttaaggtag gaatgcttgt agaaagctaa tgtgccaggt
ctactgtttg gagaagacca 27360ctctcttctt agtcctcagt cactttggga gtccattcac
cactggttaa catttctaaa 27420aaattctcag tagttattac tgactgaccc tcaagttggg
ctgccatggg tgtcctttta 27480agcttccact cactgcacta aaaagttccg ggcacctttt
ctgacacaat ctctaacagc 27540acttgataga agatggggcc atctagtgga ggaacagaaa
ccatcccttc ttccagatac 27600atagacagaa cctgaaaagc tccatcagct gcctcttatc
tttttgcaat gcatatctca 27660gacctgtagt tctaccatcc ttcctttgtc agtcactgaa
gtatcacaca tccccatgaa 27720cacagaacac atgcaaaggc gaaaaaagaa ctgcttttaa
cagcagagaa ctggatttgc 27780tgtttcaatc tgcttttaaa gcacagcgaa gaaaagcatg
gattataata ctggaaactc 27840aacttggaca aaccgctatc aataggctgg aacaagcaat
gggttacagt gagttacaga 27900aattgagcaa aacgctacaa acaggaggca ggggcagatg
gcgattggga caagggggaa 27960tagtttaaac caacagaggg gagatgtagg tgagatgtta
ggaggaaact tcttactcag 28020agggcagaga ggcgctggca cagctgccca gagaagctgt
ggtgccccat ccctggaggc 28080gcccaaggcc aggttggatg gggccccagg cagcctcagc
tggtgggggg cagccctcac 28140catggcatgg ggttggagct gggtgggctt tgaggtccct
tccaaccccc aaccatccca 28200tgattctatt taactgggac aaactgctac tatggaaata
gttaataaag caaaggtttt 28260tcttataaaa ataagaatct gcatccaatt aaagcacaaa
caaaacaagt ggaatagact 28320tgcatcagaa cactcaaagc acggtaggct ttttttcctt
tttggcaaaa gaggtaagaa 28380ttgcctttgg ctgctctgca aactgtggta actgagatta
tttcattgtt ctgtggcagg 28440ctgaggcacg cctcagatgt ctgcaaattt caatgaaagg
ctaaaatgtg acaacccatt 28500ggccagaaat gccatcattg tataaaaaca acaatggata
aatacttcag gcatcactgc 28560ttaagggaag gaataaccca gaaaatccct gatatatcaa
aatagccgct tattttttaa 28620gcaaatacag tttacaacag ctcaaaatac tgtttcaaaa
tgttctttga ttttaaactg 28680ggaaaagttc atcaaaatac ctaccaaata ttcttcctca
ccaccaaaat tacagactgc 28740tggcgtattt taacaagttg ataaggcttc ctcactgcaa
gcactggaac tttaacagat 28800ctcttacatt ctgaaccata ttgtatttaa gcgttccttt
cccttggtgt cttaagctga 28860atgtgttcct tacaattaca tggagaaaag tgcccacctt
cagttcacac tgactctagc 28920tgttcagctg agggctctgg atgagttact ggtaaaaaac
taagaaactg tcatcataac 28980tcatgagcaa caactgctgc caacacaagt tgcgtgtatg
acacgcagag caataaaatg 29040aaagctctga aagcttccct ttccagagtc aaaagtccct
gcagataaca agaatccacc 29100ttcacctgaa gtttgtgaat ttctgtgaaa acaaagtctg
cagtacaaat gtaaacagat 29160tattttagtt tcgctctcta aaaccaaaac aacagcaaga
aaaaactaga caagaaaaat 29220actatcatgt tatttataaa atgtaggcga aactccaaga
taagcaaaaa aaaaaaagtc 29280ttatctatct atagttacac tctttttaga catcaactaa
gtgtaaagta gttttcactc 29340tacagcagca tccataagat gttccttgct gccccagcaa
tgacaacgac cttactcagc 29400cgtcttgcat cttaactact gtgacaagta acattagggg
attcaatttt ttactggaat 29460cttaggataa tcttaatttt acagtttgaa ggacatcctg
agcaaacagt tgtgcagttg 29520taattcctct gttcccacgt agataaggaa tacgtttatt
tacacacatg cgctagaaaa 29580acaattacgt aatttgatat agaagaagag caccactgta
agactccgat ttaagttgaa 29640ctccaaaccg aatgctttta acagcagtta tagacgtgaa
gattgattag agcttggatt 29700acacaacatg aatacctaga gatgaggtgc atcaacttat
ggcaggagta ctcctttggt 29760aggtaatgaa gaacagcata cacacatctg taagcacacg
gtattacccc aaaccgaact 29820tggcttactt acaacaagtt ttcagatcaa gttaattctc
agagttgaag caatatgaaa 29880aacgttttgt ttttacttac ttttttaaag tgagtagctg
attgcacttt tctaacaggt 29940tgcatcagtg catcgcgtac aatgatgctt atatctgcac
cagaatagcc atcggttctt 30000ttcccaagct cccgataatc tgcttctgtt aggagattgg
gagtcgaccc gaggtgaagt 30060ttgaacatgg cagccctggc atggtcttca ggtaaaggaa
tataaattcg cttctcaaac 30120ctggtttcca aaagataaaa gcactggctc acgcaggtgc
acgatggaaa gaagtttatg 30180caaatcagta tatactttgt ttgtaaatga aactgctttt
ttcttatgta ttataaatgt 30240ttaaaaatat atatctcaga tattctgcag cctgttctca
taagtaatac catggctatc 30300ataagctaac atctacaatt taacaacgac ttccttttta
tgacagaaag tctcttcaga 30360ctgtagtttc tccaggttca ctccagagaa gtttgtttta
aaagaaaata actgaaggaa 30420aaggagtctt ttagttttta agtacatctg aacagttttc
atagaatctt agaatcgcta 30480aggttggaaa agacccacag gatcatccag tccaactatt
cacccatcgc caacggttct 30540cactaaacca tgtccctcaa cacaacatcc aaacattcct
tgaacacctc caggctcggt 30600gactccacca cctctctggg cagcccattc cagtgcctga
tcactctttc agagaaatag 30660tagtggtttt tcacactcaa agaaagagct gcccgataac
acgttcacac aaccagtttc 30720taaagtttgt aagtagagaa cgttgtagtt ggaaacgaat
ttgaagtctt actctcaata 30780tagttgttgg taggaatggt tgatacttgc ggtgcttcct
ttgaagcatc tgttctcaaa 30840gagaggacga cctcccatca gggaaatagg accgactcca
agttctgtag aacactatta 30900acttcctata ggtaagtggg cccaagccat gaaaaattaa
ttctgttact gccacgctct 30960acaagctcct ttaagttttt cggacaagaa tgagagatac
tcgttcacac tgcaaagaat 31020gacttgaaat gttaagtacc acattcgcct cttattcctt
gtatgaaact acacatgcac 31080aggatggaag cgacctctgg aggccacatg gtttaaactc
cccagtcaaa gcacggtcga 31140gttgaattaa gtacatcgat aaaatgacac tgtcaccaaa
aaggattgtt tctttagcct 31200acaaaaatta ccattataca ggttgtatca tcatcacaac
ataatcacat ttgtcacgta 31260actgtgtttg tcctttgctg ctctgcaact gaaagatcca
gctaatcaga tacagataca 31320aacgtcatcc cattagagaa aggcagttga aacgtacact
gaaagatcac acaaactgtg 31380tgaccagtac agcaaaaaca atgcttctgc attacttaaa
ttctgtgaaa ttactcaagc 31440tatccaaggg tttgctaaag ttgaaaacga tagctctgct
gcctcttacc cttctgactt 31500gcttatgttg taccttgccc cccatgctca ccaggagacc
agtcagcaac gaaacacaag 31560ttttttgctt agtcaagtgg aattagctga ctaagagatc
agacagacta caagatatac 31620ataagagaga acaatccacc acttaagtga aggggatatt
tgactcagtc cacctcatga 31680gacatgcctg caagaatcaa gtggatcact cactcaaata
gcctcaggat gaaccctcac 31740aatagttgca aatttcttag cataaacatg aatacatcaa
tcataggcca acataccttc 31800tcctgatagc agaatccaaa acccagggta tgtttgttgc
tcctaagacc aatattcctt 31860cattatcaac accaacccct ataccaagga agaaatcatt
tcaccattta gaaaataaac 31920agagactgcc tgataatgtt ttagaacatt tacaaaacgc
aagggggtaa agctgcacat 31980cttttcacat gtaagcaatg cattttatgc gtagctgaac
tcctttgatt ctgaaaacta 32040ttaaacttac cttgcatctg gactagaaat tccgttttaa
tccgtctagc agcctcgctt 32100tcattttcac ttcttgaccc acatagtgaa tctatctcat
caatgaagat aatagagggc 32160ttgttttctc tggcaagctg gaataggttt ttcactaatc
ttaaaaaagg aaacagctgc 32220agttatctta ttgtacacac aagcaaaaac atgcaacttt
ggattatgat acagtgactt 32280tgttaagaaa aagctaaaag taaaaaataa aatgaatccc
acataagata ttaacaaagc 32340tactcaaaga tacaacatcc cttcagaact actaacacag
cattaggctg agatgctgag 32400tgagatacca cagaataagg taactttagg cttcctagtc
ttgttaacac atctcattgt 32460aacatgcaga gtggatatat caaaggcgct catcacttcc
aacccatata tgcccatctt 32520ttatgtcttc aagattttgt ttgaaaacag aatgtagaaa
aaaaaccttc acacagagga 32580agaaacaaca tgtattatct gcagggctac tgcaacagat
gagccagaag gtgacaagaa 32640tcaaagtacc ccaacacttc agaccacttt gttgtacaat
cacagctggg ttcagaaggg 32700cattgatcac cattgtgctg ctaatacctt tgtccaaact
agttttaaaa acagtcttga 32760gtgctgaagc tgctgtagca caaaatacag tgcattatgg
tacttttacc tgacactgca 32820ctgaagcaaa gaaacatcta aggtttgctt taacaagaca
catgaacctt ccttccattt 32880aatttcttta gagtgtccta tctagctctg aaaaattaat
ttcctcttga taatattttc 32940ctggaactct ggaaactcca acttacttct cactctctcc
taaccacttt gagaccaggt 33000cagaggaaga tactgagaag aatgtggaat tgttcgcttc
cgttgcaaca gcttttgcta 33060gatacgactt tcctgttcct ggaggtccaa atagaagaat
ccctctccaa ggtgttctct 33120tccctgcaag aaagaaatca gctatcatca aaatgctgta
tcaagagcaa gtctatcttt 33180ctgatgaagc ctccctaatg tactaagttt tctgtatgta
cctaagaaac acctgtcaga 33240tcgatcattt acagctcagc tggagcctct gatatagcag
cataatgctc ttctcagact 33300ccgcttacac tactcacttc aacagcagta tttagaatgg
gaaataaatg ctgtaatact 33360gacctgtgaa caagtgtgga aatttaatgg gcaagataac
tgcttcttta agagcttctt 33420tggcaccttc aaggccagca acatcactcc atttcacatt
tggtcgctcc ataacaatgg 33480cacctataag aaaagattgg ataaatcact gatacgtatt
tttccactgt ttgcttacca 33540tatatttgaa aaaagaaatc cacgtgtatg tttacattaa
ataaaaacga gccatttcca 33600cacagatttc agcatcaaac agtgctactc aaatggatat
tatttctaca gagatttggc 33660aatctttttt tctttaacca caataaacca tcaataagca
gagagttgtt agaagttctg 33720cagtgtgcaa actaactctg caactgcgca gaaaacatac
caatggcaga tacagaagag 33780tacacttcct aaaaagagat caacatgacg tacaccctga
tgaagcaggc ccactacagt 33840aggatgcaca ggaaagcatg agcaaacacc ctgctgtgag
cactcagtgt aaaaagaaag 33900cctggagtag agaccaacat caatctgtat tgcatccaaa
ccagaagagg caaaaaagtg 33960tctcactaag ttgcagaaaa tgtgaacagt tcacacaaga
cggattactg tggagagagt 34020aaatatgtgc actttttatt ttccccaata tgtcaccatt
acaaaggaaa atcatggaat 34080ggtggagggt gatggaggcc cagcctgggg ccccaataca
tgcagcaatg gacagtgagg 34140tcaccgacca agcggttgtg atgtcagcaa tggaaatgac
tgtgtcctcg ctagccctca 34200ctgtacagat ttgggatctg gcagaggcca gcgtgtactt
gtacctggac ttctactgag 34260catagctgcg agactcggag cactgagcga gttggttgag
ttgtgctgtg gggctgctgg 34320cagcagttct tggtgcccac cccacagtac caccaacgtt
tcccccagcc ctgcctgtct 34380caggcagctg gggccacaca gggtgcactt gtagcagcag
aggtgagtgg tgcaggacat 34440ggcctctgcg gcggctggtg gggaagtggg agggtttgct
gctgagggac caggacatca 34500cagctgcctg cccatgggac gagtgaccat ggcctctctc
tctctttgca gttcgtaaca 34560ccttctgcct gctgcagtac ctgtgagggg agcagcttcc
cgacctcagc tctcccagcc 34620caccgcacag cccggggcca tggacgtgcc atctaactgg
acctgcccca tctgcgggca 34680aattcgggag gatgtcacct atgtgacccc ctgcaaacac
cagctttgct acggctgtgc 34740catctggtgg gcaaacaaga agccgagttg tgccgtatgc
gggcaccaaa tcaccaccat 34800ccgatactcg gtgaggtcgg acgacgacta cctcgagtgt
gctgtcccgc agcccgcagc 34860acactctgat gatagcctgc aggatgagca ggggcctgca
gagccggtgc tcatcccacc 34920tgagcacaac ttccctgccg aggtctgggc tgccttcttc
aaagaacatc agggagacct 34980cgagcccctg ctccactggc tgcaggagga gatccaggag
gcgtccagca gtgactggtg 35040ggaggtggaa gtgggacagt ggaccactgt caacttcctc
tgcgagcacg gcctggacga 35100ggaggccttg atgcgggagc tgcagccgat cactaacggc
gatgtgctgc cctttgtaag 35160gcagctcatc agcaccgcta cagccctgta cggcccagcg
atccgccgcc agctcgacca 35220ccaggaaggc cgtgctgcag gacagcggga ggacagcccc
gcagccagcc ccagcaccac 35280cacctcccat caggagcctc ctgcctcggg cctgggccac
tccaccagcc ccgcagggcc 35340cagcaccgag gagctgcccg gcagctctac tgggggaccc
gggcacccca gcaccaccac 35400cgcgccctca gcggaggagt cgcaggagga gccatggcag
gcggtggcag cgggcccctc 35460cgcccagggc agggaccgct cgtgtggggg gccccggcgc
cccccgaaga ggaaggcccg 35520cagcagcccc caggcctcgc ccccacctcc caaaaggcgg
ccccggcggc ggcgctaggc 35580tggcaccgca ctgccgtcag agcacagcgc cagcgggctg
ggaggccaac atctacctct 35640cggcctgctg cttgctggca gaataaacat cagttaaaac
aaagaagaaa atgtctctgt 35700gttattgaca agactcttgc tgttgctgtc cctacccatg
ctgctttctc tctcttccgg 35760tcctagagga gagaaatgca actttatttc caccatcata
attcagcatt catgacagta 35820ctaacaaagc acacataggc tccaaaaagc cgaagatgga
cccctcatgt tgctctaatc 35880ataatccaac caccaggact tggctaaatt cctctcctat
tgccaagctc tgggccacag 35940attacttcgt ttgattttag ctgctgagct gtggtgtccc
cctcccttca gacttcccgt 36000tagtcagtct gaagataaaa actctgttac cagatgactt
ttagatggga cagctcacat 36060ctgagctagt gacccagctg cacattttga aaccctactc
aagacaaatc caaaaggcaa 36120gagaaatctt cccaaatgaa ttaatgccaa ctaccccaat
gcttatcttt ctgtactcaa 36180gcacggtgaa ctgttcagtt gccatttttc tctacaaagg
gctttctatt agttcacaac 36240cagtttctgc tagctatttt cttgtcactt tccccttgtg
ccttcagagc tctgtgaatt 36300ggttgatggc cattttctac aatggaaagt gtaccgctac
tcgtggctaa caaataaagc 36360aagtgacatt tgttcacttt ttgtccatct ccttagagat
ttttactttt cctgcacgcc 36420tttctcatca gatagaaagg aatatttttt gcttgcaatc
tatatacagg aatccagcca 36480ctcactttta atgccctcaa tacttttgct aggttgatta
caactcagtt tttcctgtaa 36540ccaggctcca tcactaaatt aattagtagg acaagtagga
acatgagatt agttccaagc 36600tatcagttat gtggacctgg catactgtgg taatttaaat
tagcacactg taagacatta 36660cccataccag gaaacaaatg gaacaggaca tcgatcatgg
cttcctcatt ttgtaggtgt 36720aaaagaacag ctggaagact aagccaacag agcgcaaaag
gtctttaaat atcaagctaa 36780gccacttctt ttctatgtaa aaaactactg ctagctgcta
tatattgcat cactggatgt 36840gtacagcacg ttatttcaaa aacacaaaca attatgttac
tcaactgagt aacacccctt 36900atcactgcaa cacgaggaaa tcccgcctgt tgctatgaac
aaacaagaat ccatcttccc 36960gccttatcaa cttgagttca agccttcctg tgaaaatggt
cctgcttata ctacgtactt 37020ggatgacatc tgttacttgg atgacatcta ttgcctctag
gcaataatat gtcaatgcac 37080ataagagtaa aactagcaca gtctaacaaa atagctatct
gggatcttgc aactactccc 37140tttggaaaat gttttcttga taaatgatcc aatttcaaca
tatgcaccac tgaatttcat 37200ggcatgcaaa cccatactgt cataaagact gtacttctgg
atgtaaagag tatatactag 37260ttgagccacc taaagacaac aagttaactg gcaaaacaaa
caaacaaaca aaccccccaa 37320acaactagaa attcacttga ccaaagtcac ctctatttaa
ataaatggag gcttcaaagt 37380taccttgaag ctgattctgt agtttctttt tctcaggatc
ctctgactct ccttccccat 37440cactgtcatt cctgatttgg aaacaagaaa taaaacgttg
aaatacactg agaaactgct 37500gtcctaggtc acaaatcaga aagcaggaag tagaaaaaac
atcacttcga ggaatgaaaa 37560accttatgat tttagatttt ttcagctctc tacaagttta
catccttgta gtcttgtttt 37620tctacactat attctaaccc ccccctctca ctgcaaccat
ttcaacttct gtacagaccc 37680gagcccttcc tcttaacaca cttctacatg tgttgactca
gcctctagga aacaaaagca 37740tcgtggaagc agcaaaatgg cttcactgta gatgctggca
cttactcctt gtccagaatt 37800gcaactggtc ttggtcaatt ccatttagta ctacgaaact
ctctagtctt gtcagaataa 37860aggaaactgg aagttaaaag tagaaaaaag tagacgagct
aggggacaaa tggaatggag 37920acgtgtagcc tcatgtttcc ttctactata aaccagcaga
acacagtaca gctcaaaaaa 37980ataaaaccca tgaaatgagc agacaatgaa agaagctgaa
aatcagggga ggtttcaaga 38040gacaactgag cagttctagc tgttcaagac taccaaaaag
ggcaacctca cccagagaca 38100ccattgtgaa acctttcttc taccctagca aatacaaaag
aggctctgct tgttcaggtg 38160ggctgattca gctctcagat gtgcaaagtg aaaaacagat
gtaataaaag gagagcggtg 38220cataggcagg agcaaggcaa tagagcgatt cagaccatca
gaacatcagc ctatgacaga 38280accttggaac ccctcatcaa atgtgagaca gataaaactc
agaccacagt aatcatccaa 38340accaaaccaa ccaaatctgg acttattttc tagtcattaa
gtattttcat gcagaagaat 38400tgtgttacta ggctcactgt catcgaaaca aaaagtatta
gtgtaaaaca gctttcattc 38460ttcagtgaat gtcctacaga agcattgaaa gatgtagcaa
acaagcacaa aaaagcccat 38520aatattaact cacattattt ttccttttta agcccactgt
ccttcagcat tagtagttac 38580ctgaagcgaa gcacttcaaa aacactactt aaaatgatct
ctgttgagat ctaagttgaa 38640tcttagaata agcggagttc aggaagtatt ttgctttacc
ttctcccaaa acataccctt 38700ttccatcggc aggaccagac tctttaactg gctttggtgc
agttttttct ctctttttca 38760gatattcttt cagtttttct gctctgtcca agtattccgc
acatttcact ctaatgctct 38820gttttgcttt atcaccctgt gtttcatcta agagtgtgaa
aagaaacaat gcgttgttaa 38880caacaaaaca cacgtgcatc attcagaaaa catctttatg
tgttatcaag atacctctct 38940cagggctcac cacgcatcca aatgtttcat ttacttattt
tttcccctat gccatggaaa 39000gaagtgacag gaaagaagtt aacgcctaca aatcaatggt
aagtaatcac tttcaaatca 39060aatacacacc tgaacgttgc tttgccttaa aaacttgcct
gaacacgagt aaggacagtg 39120gcactggaag ctttttctgt cagtctctca aactgctata
tagtgtctta actacttttc 39180taaactaagc cattgagagg ctgacttctt gtttttagag
accttttttt aatctaagac 39240cactttattt ttccccggcc tgctaatttt gaaagttgtg
cacatcaaag gaagaaaaaa 39300gtcacaaaac atctgaaaaa atgaggagtg gtccaacagc
cacagttctg ttagtcgcta 39360ctgcagtatt ccagatcagc aatcaagctt gaaaatatta
agttcatgcg ctacgttccc 39420aaaagtccat cagtatggtt aaaagcatag ggaagtaagt
ggcatgagtt aatgagcaca 39480aaacaacctg tggatactac taagagttct tacaagaagg
gagcaggcat gcaatatgca 39540acttttgtcc ttgctataat ataacacctc agccaaacta
cagagagcaa gtgtcaactg 39600acaacaacag tcagaagtta aacgttgatg tcgacagagg
agactactcc gggcaatata 39660aaacttgact tcatcacccc atgcattaca cttacattta
acaacgtgga ttaaatactg 39720cacagcatgc tggtacaaac ggaaggcttc ttcatagttt
cctgctttat cttcttgtgc 39780tgccttacta gcgaggtcta tcgctttctg taacataggt
aaataattca aatgagtgtt 39840gtgtgagtgc tttgtgcgat caaagaggtt tttaagctgc
tgctctgacc gcttcttggt 39900ggccagcttt tctgctcctt gatgtttacc caaaagagct
gctgttattg aagacttgct 39960gtcagttgtc ttcatcaaat cccatcggca tcagtgttga
tactggaagt acacgattac 40020aaagcaatga aagcagcacc ctttcccttc tgacccagtg
ccaggagttg gtttcaaaga 40080ctcattattt ggtaagcttc tcatgaaggc tttaggtact
tgacgtacag aagtgagaaa 40140ttctaaccat ctcttcagtg tgcatatggg ggggagctca
gtggacagga aacataccta 40200aattatcaca gaagttctat caaggacaat ttagagatgg
atttttattt gtttgttgag 40260ataatttcaa atacatctgg tcgtaatcta agacactaca
tcggcctgta gatatattga 40320tattactgtt attcctttga tcccgagtgc tttttattac
atttcagatt acattacaga 40380tttttattac atccttggaa catccgtact gcttcaggac
aattaagaat gacaattcca 40440atgactaagg cacgtatgct taaaaaagcc agagttgact
aacgctacct cgaacttcta 40500cagccctgtc tgcatatttc caccttctgc cagtttattt
cccaaaggca gggacagcgt 40560gctcgtgatg actgtgctaa catcagggag caaggtgaag
atattcaacc tcatcacagg 40620gttttcacta cacactgctg tgcacatact ctcaacagta
accagacgct ctgatgcatc 40680tcagtcaaaa ccgagcagat aaactgcagc catcagagaa
ggaggaacaa catttctcct 40740tctattgttt tgtcttgcct ttttggaagt agagatcacc
tcattggatc catctgaaat 40800caagagtaat ttatttcaaa acaatcacct gacaagtaag
actatggatc ctttgtgaca 40860agtgttgaaa acagagcaac catctgtttc tttgaaacag
aacttggtct ttcctcactg 40920ctgacctcgt gctgccctct acaaattcat tgtagagggc
aaaccattca aattcagcac 40980aacaaaaata aattccaagc aataatttct gttactttag
tgatttaatt accacaggaa 41040cagtccaatg attcctggat gcagaacaac aaaaacaggg
ctatgacaaa aatgacaata 41100tatccaaaca acaaataaga gttggacttg atgatccctg
tgtatcactt ccaacccagg 41160acattctatg atgctatggc tctgtgttct aaatggcaaa
gaccgcctct gttcaatggt 41220aactctctta acagggcatc ttagagccct gctcctctga
aatacaaaaa caaaggtcta 41280catcctgtgc tgactgtttt tggtattttt tcaaataaaa
acccagaaaa ccatcacttc 41340ggttttagac tctcagctct ggtactttat tacattagga
aggctcttag cctgctactg 41400caatgaaaaa caccagtaac aaacaggaaa taatttatga
aagttgtatg aaataaggca 41460tagctgtaac cataaatgag gcacaacctg tatctatggg
gctatagttt gagagctgga 41520tgaacaccac cctcagaacg aacatcggct ttgctcttct
gcttactctg ggccctctga 41580tttcacagaa gggcgcaggt tggaagggac cgtaaagccc
attcagttcc aatccccctt 41640gcatggtcag ggctacaccc caccagctca gtccgcccag
ggccccatcc aacctggcct 41700tgagcacctt caaggatggg gcaccacagc ttctctgggc
agctgtgcca gggcctcgca 41760accctctctg agtaaggagt ttcttcctaa catctaactt
taatctcccc tcttttggtt 41820taaaaccata cccctttgcc ctacctctat cagaccatgt
aaaaagtcag actccctcct 41880gtttataagc tcccatcaag tactggaagg ctgcagcaag
atctctccca gcttggtcac 41940tataagcact acatagcctt aagcttacag gcatggacat
ggtttaaata ggtttaaaac 42000tactttttgc acagattatt cctggatcta ttttgaaccg
gcaacacaag cagttcactc 42060ccacaaccga aggctaaaat aaaataaaat aaaataaata
ataattaaaa aaaaaaaaaa 42120aaggaataga gagcagacaa gcatttccaa gagtcgtact
ctcagcagaa acccagtcca 42180aactacgcct ccagctcaca gcaggccgca gtcttgcctc
agaggccaac gggtcttctg 42240gtcccagccg ggcaggtgac tacccggggt cctccggcgc
ctccgagccc ccacccaggc 42300ctgctcgacg ccccaccgct ggtgtcagcg cttctgcccc
caggcccagc ctggcgcccc 42360accccgccga gcccgccctc ccacccgccg gctgcagcgc
accggggttc aacaggaccc 42420gctctacctg caagttgccc gacatggcgg ggagccggga
aggggaagga cacgagacga 42480cactggctac ggccgaccgg agctgccctt cccgccaccg
ccgcccaccg aaccgaaaag 42540ccggccttcg ctagccgctt ccgcacctca gcgccggccg
gcccgcttcc gcttccgggc 42600agcgccccgt acgcgtcact tgacgtcagc acgccgcgcc
tcgccccgcc ctatccgagg 42660ggctgagcgc atgcgggccg ggcgccggaa gcggaagttc
gtgggttggc gcgcagcagt 42720ggtgctgagg gaatgggggt ggtgttaggt ccagcactga
cgtaggggat agggctgaga 42780tctgatcatg acctactgtg gggagcctgc tgtagcagag
gttgggctgg atgctctcca 42840gatgtccctt ccagtccctg cgattctatg atcatttctg
taaaatgtta aatagtcact 42900tatagggttt tgaataaatc acgttttttc ctcatgcctc
acgtttggga cacaaagaca 42960tttttttctt acatctcttc tttctcgtac catttgcttg
ctttcagcgg cactgtcttt 43020tgcataatct gagtgcagaa tgctttttat tcacagaacc
agctcttaat aattcctgac 43080agtcataagc agtcaggcgt tagtcacctg cagctcagta
atgaaactca actaacaggt 43140ctgcagagta agagcaatga cgtgactcag aaagcacagc
acattgtaaa caactcttgt 43200aaacttgcta tatgggtttc agactaatga acttctgcta
agtcggtgca acagttgtgt 43260taaattactg tcatatcctt ccctatgtta ttgtaatact
gttgaggaaa tgcttcctta 43320gattcacaat cctcgttttt ctacctgcct ccaactaagc
ccagtacagt ctgctctggg 43380atgaaggtaa aaggcacaag cacagtcagc cctatatcta
ggaaggttga tgtaatttct 43440tcctaaagtc ctctgcttgg cagcttgttt tgcttaatgt
cttcatatgt gcacaccagg 43500caggatgctg aaggctcgtt gtttggggat gatcagtaac
agctgttctt ctattgcaaa 43560tgtgaaaggg tacaatgtag caaaaattcc tggatgtaat
caggctctgg gaaatgagaa 43620ggcaaaggaa atgttggagg taagagcagc gttcaggaac
cagaatgata tgggttggaa 43680gggatcttaa agatcataga atcatagaat cgctaaggtt
ggaaaagacc cacaggatca 43740tccagtccaa ccattcaccc atcaccaatg gttctcacta
aaccatgtcc ctcaacacaa 43800catccaaatg ttctttgaac acctccaggg tcggtgattc
caccacctct ctgggcagcc 43860cattccagtg cctgaccacc ctttcagaga agtagtattt
cctaaagtcc agcctgaacc 43920ttccctggcg cagcttgaag ccattccctc tagtcctacc
actagtcaca cgagagacga 43980ggccgacccc cagctcacta caacctccct tcaggtagtt
atagagagca ataaggtctc 44040ccctgagcct cctcttctct agactgaaca atcccagctc
cttcagccgc tcctcataag 44100gtctgtgctt cagacccttc tccaactttg ttgccctcct
ctggacacgc accaggctct 44160cgatgtcttt cttacagtga ggggcctaaa actggacaca
gtacttgagg tgcagcctca 44220ccagtgctgc gtagaggggg agtcatcttg ttccaaccct
gttttcctgt aggtagtatt 44280tctggctgtg ccatctgtac ctatggtttt caaatctgta
atgctacacc tagcttttag 44340acctaggtct aaaacagtac acaagtcaca ggcatgttag
taatgcctct ccagtcacac 44400tttgcagtct tccgaaactc cacatataga catgtttcta
tgattgtgaa tgagattaaa 44460aaaaaaataa attaataaat cagaaaaggc acgtgtatat
ttacagataa caggctaaat 44520attatacttc ttaattaagc tttactatac agtattcctg
ttatgtgact ttgcagctag 44580ttttgcctaa ggaaatactg gctgaatgct gagtaataac
atcacgacag actcctgagg 44640agctaatgaa gtattacacc aagagtgtag cttcagtttg
agagacgtgt atggtcacat 44700tttggaatgc ttcccattgc tgagttgctg tgttacaata
ttctcaaaat ccgtgtcagt 44760tattgtgttc aactgagtgt aatgacaata aaatatatta
atgacgttaa atgaagatat 44820catagaatca tagaacatcc caagttggaa gagacccaca
gggatcacca tgtccagctc 44880ctggctccac acagcaccac ccaaaattca aagttgatgt
ctgagagcgc tgtccaaatg 44940ctccttgaac tctggcagct tggggctgcc ctgggcagcc
tgttccatac ccaccaccct 45000cttgttccct cgggctctgt cgcagtcaca cagagcagag
ctcagcgctg cccctccgct 45060ccctgcgagg agctgcagcc gccaccaggc ctcccctcag
ctcctctgct ctgggctgaa 45120cagaccaagg gctctcagct gttcctcata cacgttgccc
tccagatcct tccccatctt 45180tgtggtcctc ccttggacag tctctaatag tcttatgtcc
ttatattgtg gcacccaaac 45240ctgcaccctg tgctggaggt gcaactgcac agcacagagt
agagaggaca accctttcct 45300gcactcgatg gcagtgctgg gcctgatgta ccccagggta
tagttggccc tttgggatgc 45360tagggcacaa cgctcagtca cattcaactg tctgtcaaca
agtacctatt ggcctgcatg 45420aggcctgtct gctaattggg actctattaa atcacatcac
tgtgacacta ggtggcacag 45480gcacacatga tctccatgtt ccttaaggct gagtgaatca
tggagaatgc ttcctgctat 45540cagtttttgg catggaaaga gaggagccaa accaccggtt
ggttcaatgc cttgtgccag 45600gaataggtga atgcatcaat acaataagtc acgtctacag
cacagccagg cctcatgtca 45660gcaatactgc tccactgtga tagctgaaag tgactataaa
tgactaacgt tagtgtggga 45720ctttggtgtt agatgacgtg agagccatgc agtgaaagag
aattagtgtg gcagagtatc 45780taacagtgca ggtagataag gcaggaagga taagtgtaag
gaaagataag gagaaaggca 45840ggaaagtaaa acctctgttt ttctctagtt ttctacctgg
tgaaatgatg aagaaagatc 45900agtttgacat aggttaacaa aaactgtcag taagaaaggt
aggagttaag atgcatgttg 45960tccaaatccc actacattac tttgaccctc ttcagcatat
gcacaatgag atcacttgcc 46020caagacagga cctccagtgg gcatgaaatc tgaaaatcaa
ttatttgcta tttgtgttgc 46080ttatcatttc cagatgaaat tctacacgag ataattagag
tgatgtcctt gaagatcaac 46140ctttttgtct aattaaggta tttgctatag cttccagatg
tattgcttat ctatgataaa 46200tatccttcct aactacaagg cttctataat aagagtaacg
tcctctatag taaccagtag 46260aaagtaggtg gaagctgggt gttcttagac aacctgtgcc
catacatgga caaagtgagg 46320aggaggacac ctccctaaat gaccaccaga gaccactgaa
gacccacatg caagcacaga 46380agattcagat gtgttggtgt aaccttgtaa acgcagtaat
ctcgtgaata tgtgatagat 46440aggtgtgcct tatgtattag ataggcgagt attgagaact
tttggtttat ggatgtggat 46500agtgctgtta tccatcttgc accctgagca taaataaagc
aatatctctt ctatagtgcc 46560ttgtcttttc attgtatttc aggagacttt gaaactgaca
acaggcatgc agcttgggag 46620tgctcacagt cagtctggcc acagtgcctt caagcctccc
ctgcactggg atgtggtgtg 46680acaaaaagca caaacactgc ttttgtagaa gacccagacc
acaggctgca ctagggaacg 46740tgtctgcctg gagcacagtg ccctggggag tgctgctggt
acagtagtcc tggatgagtg 46800gcttccttct gtaacctttt aattgcacta gaagtacacc
agcatggcag agaagggctg 46860ggtcctaaga gcccttcttt caaattcact cagaactcca
gatgtttagg cagggtgttg 46920tagctgtaaa gtccaggaag aaaaggttta aagctgtact
cggcaccaga aagactggag 46980ccaaaataaa gccacattgc acccatggca ctataggcaa
agggtagcct tggggcaaga 47040cttgatgtac tagaagttga ggagtcctca gactctgtgt
caaggggatg tgccacaact 47100ctactgtgcc cctacctgaa gcctgaatca gtacaaatgt
ctcacgcatg ggttaggcat 47160ccttctctca aagctcttgg tctttgcaca ctttcttctg
cagctgcagc agcagccaaa 47220ggaaaattag gtcttgcttt gaaagccagc cccttccagc
catgactggt cccttctcac 47280tccacatctg tggatgatgc tcccacagca ggtgggagag
acagaggctt tcttgaagaa 47340acccagcccc tctaggggaa cactgtaaag tcacagggga
ggagacgtgg ctttgagaca 47400gtgatatact ccatgcccct ggcgttcttc ccctgagtgc
cactggtgct gctcagtggt 47460cacatgccac caaagtctgc attcatcttt aaatgctgct
gagaattcaa cctttgataa 47520atcatctgct ttgacaaaat cgacatttaa aaattaatat
ttcctcttcc atcccctact 47580tttacaggct ggctcaagaa aatgggaagc ttaatgtaga
cttgggtctt actaaaccat 47640ttcactggga aagacattca cagtctgtgg cagatggtag
cagtatattt tctctcatag 47700tacaggaatg ggtctggtag tacctctttg gaaaggaaaa
tgtaaactca tacgttttga 47760gccaaattcc atcagatttc ttagttttgt tagttttcac
tccactcctg ctggaaactg 47820aaaatatgga aatgcttgga aatttactgt gatttgggtt
caggtgtgtg tatgcaggaa 47880atgtgttacc ttccagagta agtcagttta ttctagaaat
gggatgactc cacttttata 47940cacttgtaat tcacagtgag attaatccag ccaattggga
aaacagcctt tcttaaattg 48000tgaaaaacat gctccacttc tatgtatttt ttaatatact
tcagcattgt gaatttgaag 48060tttttcttct actgttacat gcattccaac agaatttgtc
aggaacaaaa atgaaatctg 48120aaataatatt tttcttagct ttgcatgtgt tatcctcaag
ggtaatcact gtcctaaaca 48180acatacttat ggctgtttct gagcctttct tcttcatgaa
ctcatcagaa agggacactc 48240atattggcag tctgtataga gagccaagga caaatatttc
gcctacgtct tctctgcgta 48300gcattttata tattaggtct tgctagtgaa ttatgactga
atggaataca gtcccttcag 48360tgatgacttc attcatgatt gaataaatgt agcttcaggg
ctgtatggtt gacttacatc 48420atccaatttt gccatctgca acagccaaca cctctaccca
tatatgaatt cagcgaggga 48480ttttgtacta tgtgttgctg ggatgtagca gcatttctct
ttgaaatgtc tttacagatg 48540caatgcctag caggcttaac agccctacct gcttcagaga
cactgctgta aaaagaaaaa 48600gagaagcttc ccagccagta tttcatcaag ttaaaaaaaa
tctaaaagtt tatactgtac 48660catttggatt gctgcatgtt gacatcattt aggattctga
aaacctaaag aagctttgga 48720gcaactccta agtgtatggt agatgctctc attatgtaag
agtgacaaat cactaccagt 48780cttccaaaaa tgcatgctga aatcaaaaaa gaaataatgg
atctcacaaa actggatctg 48840cagatcaggt tctacagcct ctggtatgca agggttaaag
tagagtgatt gttgtagctt 48900gtgtctcaca gtcagacata aatctgtaag caggtccagg
ttttgtaaat tgttgcttat 48960caccacatga gcaataagta atctgaacac ccaatgtaac
agatttctag gagttagggc 49020tgaaagcatc atgaagttta ttcttttcta cagcaaagca
ggctctgtgt acctgtctag 49080ccacattgtc tctgacaaaa tttatcatca attctcatct
ccatcaactt ttaagaatta 49140cagaattgaa gggagggatt gttgaaaggg atctctggag
atcatctagt cttaccccat 49200gatgaagcag gttccttaca ataggtggca taggaaagtg
tgagcaaaca ccctgctgtg 49260agcactcagt gtaaaaagaa agcctggagt agagaccaac
atcaatctgt attgcatcca 49320aaccagaaga ggcaaaaaaa gtgtctcact aagcttcaga
aagtgtaaac aattcacaga 49380agatggatta ttgtggagag agtaaatgtg tgcaattttt
attttcccca atatgtcacc 49440attacaaagg aaaatcatgg aatggtggag ggtgatggag
gcctagcctg gggccccaat 49500acatgtagca gtggacagtg aggtcaccga ccaagcggtt
gtgatgtcag caatggaaat 49560gactgtgacc tcgctagccc tcactgtaca gatttgggat
ctggcagagg ccagcgtgca 49620cttgtgcctg gactcccgtt gagcatagct gcgagacttg
gagcagtgag cgagttggtt 49680gagttgtgct gtggggctgc tggcagcagt tcttggtgcc
caccccacag taccaccagc 49740gtttccccca gccctgcctg cctcaggcag ctggggccac
acagggtgca cttgtagcag 49800cagaggtgag tggtacagtg gggaagtggt ggggaagtgg
gagggtttgc tgctgaggga 49860ccaggacatc tggacagctg cctgcccatg ggacagcgag
tgaccatggc ctctctctct 49920ctttgcagtt cgtaacacct tctgcctgct gcagcacctg
tgaggggagc agtttcctga 49980cctcagctct cccagcccac tgcacagccc ggggccatgg
acgtgccgtc caactggacc 50040tgccccatct gcgggcaaag tcgggaggat gtcacctatg
tgaccccctg ccaacaccag 50100ctttgctatg gctgtgccat ctggtgggca gagaagaagc
cgagttgtgc catatgtggg 50160caccaaatca ccactatccg atactcggtg aggtcggatg
acgattacct cgagtgtgct 50220gtcccgcagc ccgcagcacg ctcagatcac ggcctgcagg
acgagcaggg gcctgcagag 50280ccggtgctca tcccacctga gcacaacttc cccgccgagg
tctgggctgc attttttgat 50340ggacatcccg aagacctcga gcccctgctc cactggctgc
aggatgagat ccagcagttg 50400accagaaatg ggtggtgggc agtgtgtgtt ggacagtgga
ctgttgtagg cctcctttgt 50460attttcggac tggacgagga ggccttggtg caggagctgc
agccattctc tgatgctgac 50520ttggtgccct ttgtaaggcg gctcatcagc accgctgcag
ccctgtacgg cccagtgatc 50580cgccgccagc tcgaccagca ggaaggctgt gctgcaggac
agcgggagga cagccccgca 50640gccagcccca gcaccaccac ctcccatcgg gagcctcctg
ccttgcgccc aggccgctcc 50700accagtcccg cagggcccag caccgaggag ctgcccggca
gctctactgg gggagctggg 50760caccccagca ccaccaccgc gccctcagtg gaggagccgc
aggaggagcc atggcaggcg 50820gtggcagcgg gcccctccac ccagggcagg gatcgctcgt
gtggggggcc ccggcgcccc 50880ccgaagagga aggcccacag cagcccccag gcctcacccc
cgccccccaa aaggcggccc 50940cgacggcggc gctaggccgg caccgcactg ccgtcagagc
acggctccag tgggctggga 51000ggccaacatc tacctctcgg cctgctgctt gcagataaaa
tgtggggatt caagaaagaa 51060tatttagagc acaagctgca gaacaagata aacagcatgg
gaaaggaatg ctgaggacag 51120aggatgcctc caagagagaa gaaagtcaag tgagctgcat
gatcgctgcc taacaatcct 51180aattggaaga agagtatgtg gctaggaatg actcataact
ctgattggag aagcgcctgc 51240atgcgtggtt aaggagtaga acaagagcaa gggtgaccct
gtgggatgtt ttgttgacat 51300gtaaaggggg tgggaaagat accagagaaa acttggcagt
gtatttaagg gatattagaa 51360tatgcaataa atgatttgga ttgctcatac atctgagtcc
gtgccttgga tgctgcaaga 51420aaataaacag aaattcaaaa aaaaaaaaaa aaaaggataa
gaaaatgtct ctgtgttatt 51480gacaaggctg tgggcgttgc tgtcctttcc catgctgctt
tctccctctt tttctcctgg 51540aggtgagcac agacatgcag ctttatttcc atgaccataa
attggctttc atgacagcac 51600taaaaaaaca cacgagggct ccaacaaaca gagaaaggaa
cttatgttac tctaataata 51660atccaataat cagggcttca ctaatttcct ctcatactgc
cagctccagg ccacagataa 51720ttaagttttg tttgatttca gtgactgagc tgtgatgtca
ccctctctgt agacttccta 51780ttagtctgat gttaaaaaca ccaaaaatat gtgctgtaat
ccaaagagaa attatgggtc 51840ccattaaatt ggtactttgg gttctacagt ctctgttatg
caagagttca agctaaatga 51900ttgctgtagc ttgtgcacga gttttgaaaa gataccaatc
tgtgaacaga cccagatttt 51960ctttctggaa ttctcctccc ctgtgcaaag gaaagcacat
tgttttttgc tctcatcaga 52020gagtactctg aaatgaacat ttttgagtta gacagtgagg
agcagaaaag aaattctatt 52080cacataggtg cttttaaaag cattaccaga ttcttctaga
caaatgacag aggaataact 52140tttgccattc cattacacaa tagaataact gaactgcaaa
acaaagagtc acgctacagg 52200agtaagtttt gaaactgact tgcttacctc tgatgcttcc
agctgacttt ctccattctc 52260acagtagatt caaagttctt ttttttttaa ctgtgtgact
gtagagagta gtgttcacaa 52320cttaactgca tgctgtgcag tctgaattag agctgggggt
aggtgataac acacctcctt 52380caactgtttt gttttcctga actgtggttt gtctcattat
tttcttctaa atgctatttt 52440aagcagtaag agtttaaaca tgccttctgc ctgccttaga
actgcagaag accttaaatg 52500cagaactctt actgttcttg aattcatggg aaggtctgag
gaaatggggc catccaagat 52560gtcctccaaa caatacgttc cctcattcca ttatgtgtaa
ggtacagtgg tgttgtacca 52620gggggtgagc actgcagtgg taagtgctgt tggacctgtc
gtgcaagaat agaaagaagt 52680cccacaacag ccaaagtcca gtggctggac caggagtagc
caactatgtg gctgctgtga 52740tttgatccac accagatttc caggttagca ttttcctctt
agacccatcc ttattaatcc 52800ctaagccttt taattagttc ttgtatggaa agtagcagaa
actgtatagg aagtcattta 52860tctttctctt catcctagcc actcttacca gagtaatttt
catcttaagc aggaagctct 52920tcaagccagg ctattattcc atcataaact gtctataatt
cttctacacg tatgacattt 52980tgtctacatc ttccaatatc tgtctcacta acaagcctgt
ttctgttttt tatccacaac 53040ccatcgaatt tggtagccat ctttgcagtg ggctttggat
cttgacccaa gaaaggaaaa 53100cggaagggta tttgcactgt cacaagttcc tatagaccta
attgcagctt tccaagtcac 53160ttatgcctgt tatgtaaatg ttaacgctat tgtggagttt
attaactcgc tggattatgc 53220atgaagtatc ctctggagtt tccccatcaa gcttaatggg
accattagat ctcagaagaa 53280tgacgaaagc tatttctcag tagcttacat attacctggg
tagatgtaat gggaaagaga 53340aaaagaagca ttctgttatc aattcctagc actttctttt
gttaaatata ggctattttt 53400tttatcattc acaatttttc ctacttttcc tttttttatg
gcctagtatg ttctgtgctt 53460tgttacacaa atctagggat cctgggttag tggtgatatg
agctgaatca gctgctgaat 53520gtaggaatag ctcacttgct ttcatgggtg ctaatcagtt
tacattagct gaggttcagg 53580gccattgttt gttaagattt acatctggat gtcaagatgg
gtttgcaggt ataactttta 53640taagtgactg gtgagacagc gacactgtag ggtgttttac
ttcgagtaat gcagaagaat 53700gtacactgat tttgttgcct ttagcagatc tgccaaatac
caactgaaga agcaagaatt 53760aacatgtttg ttcctcgtct tagttgcatt cagggacaag
aaaagctcca tcctctcctg 53820aaaatacaca gctggagaaa attcagacca tggaggcaga
cccatttcca gtgtctattt 53880cagcaaatat tgactctaag cttttattgt cctttaatat
gcatatattc atgctgtgaa 53940tctatgctga agaactctgg gaaggtgtgt gccctcaccc
acattaatca cccagacact 54000tcataactac ctggattaca ggagaaagtg actcatctac
tgatgacgct ggataaaagc 54060aagaggggaa agaaatcccc agttctcaca cctccctcct
ctgcacatag taaggaggca 54120ctcaagggca taatgcaaac ccagagctgg aagggaggct
gtgtgtcagg gcccaggcct 54180gctgctgtgg gcagcaaagg ccatgtaatc gctggacatg
tcagttccta ctgctccgat 54240ctgaaaccag ttcagagtta gagggagagg tctgcttggt
ctctctgcta ctcatgggaa 54300aagcacttct gccaatgtga tctactctct cttcaaagtt
tccacattgc ttacgtgagc 54360aatcctatcc ccatgcaggc tttcttttgg taggtgagcc
cctgataatt cgaaaatcac 54420acctacactc agctaggagc taaccagatc tataatcaag
cacagtatgg tgtggctatg 54480tagaggaagc atcttcaata aatgtactgc agtggtaata
tgcttttaat aaggcaactc 54540tgttcacatg aacagtacta gagagaagca caccaggccc
tgaaacttca gggcaaacaa 54600aggtttgaaa gtatccctga attaaaataa ttgaggaaag
gtgacaaacc taagcatgtt 54660tgggtttttt tctgagacaa gcatcgtgta ggttgttttg
agccctagtc acagcctggc 54720aaaaggaacc tggtgcagtc actatgggca ccagagagga
aggaagaaca gtgttgtccc 54780tgtccttgtg agaaggagct ctgaaccaca gtgcacatgt
gtgggggttc acaatactgt 54840cttcctggaa ggactggatg cttcagtggg aagtgattaa
tccaaagcac tgtctctctg 54900catctgattt atctgtgcca taccaaggcc agttatgccc
agtgctaaga gttgggagca 54960atgtctttag ggaaaaggtc agatgccaaa tgatctgatt
ccagcacttt catttcatct 55020ttcatttcct gttctccagg ttaaaggcct tgttctcact
gaaagctggc aactgtttgg 55080ccgcctgtta tttcagagtt gttttatcat tattattatt
ttcctgatga gatgtatatc 55140ccaaacaaga acaggctcaa taaaataaat gaatgaaatt
aatttcctgt ctttgattag 55200aaatattcac tggtgaggca cacttctacg tcagcagaca
tgtctgcaga aggctgagtt 55260cttgctggac gtgttgaagc agtgtgttgc tgtgtgacac
catccctcat ccatctcagt 55320gcagatgcct tgggaagaaa gaggaaaaaa gagaggtcag
cttgctgctg ctcagcttgt 55380gtctctctca gtataatcct agaatgacac ttgattattc
taagtgctat tgtagttgca 55440aatcatcgtg tggtttgtaa ctgtcagtct gacctttact
agacatatac tggaaaatat 55500tcttgcctgt gacttctctc tattgctaaa taatgatcta
gacagataca cagtgaatac 55560agaaagttca gttgtataga ccaactgaca gacattgtga
ttttaccctt tgttttttct 55620aagtgtgccg aggaagcagg ttgttgtatt gaaataaagg
catgcaaata acctgctact 55680ggctccctcc aagatctcag gcttgctgta aaagccgtag
ctaggtcaaa aggggttgca 55740ccttttgtga ctggcagcat aataaacatt ccccagttta
ttctgctcat tattccatcc 55800cacttgtagc caatttcctg tgtggtctcc aaagcatgaa
atctgcaatc agacatgtcc 55860tgagtgtcaa tgcattaggg aaataaaata aggaaaaaaa
gacaacagcc gtcagttgga 55920gtctgtgaag gagctgagct ggttcataga actgtgttga
gcagcaggag cgttccttgc 55980cccaaacgag cctgtccaag gggtgggagg aaggaagctg
tttttctttt ccactcagca 56040gcgtgtagca acaacgccct gaaggagtgg cagggtgagc
cgagacctgg ggctgagagc 56100agacaggatg acaggagtgt taccaggtgg ctgcatccct
cctgcacacc gcatggccag 56160gtggtggcac atggggatgg ctgctgcgtt ctggtgccca
gcaaggcctt cgcttgtgtt 56220tccccttggg ctgttgaaga cctgaaaatg ttggtgacct
ggaaaaggaa gatgaaagct 56280cctctgttgc tttgtcagaa gactgtggct tgtccttgtt
atgaaacttt ggactgcaat 56340aatgacggtg tttcactttg cacatccctt gttcctatgt
tgttttgcct ctgtcttttt 56400tcagagacag catcatgaca ggagatgtcc aactcacagg
gagctttaaa acagctttga 56460ttttattatt attattatta ttattattat ctagtaaaga
aaaatcctgc tcttcattct 56520gctatctttt taacttgatt aaaaaacagg ttgcaataga
tgtgtgttga aaattcttgg 56580aggtcaaacc aagcaagact aattcggtga caggtaaatg
caggagatgc acaagctgat 56640gcagttagtt agatgtcatt cagtcagact gaggaagatg
aactgggaag ccaagctcca 56700gtgcttgtcc cttgcaaacc tcaggtacct aaggctcagc
atcttctgct tttcaagtca 56760gcatcttttg ctttactgct tcttcctggt tgggccataa
gtaagatcca ggtaagtgac 56820aggcactccc ataaatacta atgtgaagat acatataaca
tatacataaa atgactttag 56880gatgttattt gtctctatat gtgacaccta atattatttc
aacattatct ccaactgtaa 56940attaacccca aatatccatt ctctggggaa gcaggactgc
cctgtgagcc actcagttac 57000aggagcccgt gtggactcct ttctttgaag catggcaatt
ggttatttct ctcatggcac 57060tatgatcaga aatcagtcat ttctcagcag cagctgggtc
aggcagaaga gttctgtaag 57120cctacaaaat gccagacctc agctatcaaa gagaagttac
agagtggagc gcagtaatgt 57180tcatgcacct tcagctttgt aatgtagagt tctttcacct
ttccctgctc agcttttaga 57240agtaaccagt gcaccccctt gtatttgtat ttccagccta
catggtgatg gagctggtga 57300tggtgatagg aggtgaaggt ggtgacctgt gctatctctg
tcaaggcagt tgagttgtca 57360gccactcccc accagtgtgc atccagaagc tcctgaacca
cttcacagag ggccgtcttt 57420agtagacttg ggcctgaagg aaagtttgtt cctttggctc
tagattacct gcagacatct 57480gagaagagtc tggcagccaa cattattgca gatttttatc
aaaatttctc tcatagtgta 57540aggcataatt agatatgtga caataattgc ccagatgaca
catttttgcc agctgttctc 57600gatgttcttt gaattgctta aacatatcca caccttaatg
tttgttatga attcacttat 57660ggtaaagcat actaatttta tgttcactgt ctggtaaata
aatgaagttg aaccctactt 57720catcacagca gaaaggaaaa taagatttcc tttgggtaat
aatgagtctt ttggaagcac 57780atattgctgg gatctgatca ggaagcagtg gtgtgattta
cactcaaaac tgtcattcca 57840gaagtagata actcctcagt tgccactgcc accaaggagg
tatcacatca gggaagacac 57900gtactttggt ttcctagccc tttcatttgc cagcatagca
atcttgaaag caagctcaat 57960aacgtttact ttttttgtag cacatcagta gtctaagggc
atatagggct gcctggggtg 58020ggtgaggtgt ggacagcagc tgtgtttgtg ggtggcagtt
tcctcatgaa ggttgacttg 58080gactccagga agtccttgtt agtgtggtgg gcagctagac
ttcgtgatgg gagaagcagt 58140gtggagcaga gtaggccacc ttttccttgc taaggactac
atacattttt caaatcaaat 58200aagactgtca ggctgcacca ccacctttcc ctctcccttt
cttcaacccc ctccgcattg 58260gattcatcag taatcatgtg gccaatgagg cctgaaccca
gtggttccca ttctggagat 58320gctggaaagg agctgtggct ttttccaccc tcccagctga
aatcatctgc ttgagagcaa 58380cacggtgagc agacatttct ctttgagcac gagccccact
ctggccttgg gcttttgagg 58440caaccaagta caccacacaa ccctcacaaa gacaacactg
agacactatc ttaacgcaga 58500cacagatgca aagtactggg ttgccatcac acgttttggt
tgccttccct gtgtgtcttt 58560ttcacaatta caatttttta cgaatcacag caattccaat
gtaccttgga gcactggttt 58620tgctcagaag ctccttcagt tgctccttca ctcctgcacc
ctctctttca gagtctttgc 58680tccctcctgt ctggtgccta ttgtagggca cttgctgcag
gcaacgtgcc gaggctgctg 58740tttcctgctg gagcatgttg ttgtgttcag tagctaggca
ggcaaagaca actcaggtgt 58800gactcatact gctcctccag tttaggagtt gcttgaagaa
tgattaaggg aaagaaaaaa 58860aaaaaaaaag aagaaaaaaa aagcgagagt gtgtgcgtaa
ttagggctgg gaaagtggga 58920gtggtgtcaa tgagcaggca agctaacaga ctcttctgct
cagcaaggtt tcgccacagg 58980tctctagttc ttcgtgttgg acccatttca ttgattcatt
taccaggatt atacccttgc 59040aaagtaagta caacattttt tcttcctctg gtactttgat
gcctaactaa cagttataaa 59100atctctgccg tgaaacgtaa tcgcatagag tgagcaggat
aaggaagctt gtacaaaaag 59160acagcatgtg aaactaaagg tggaaaaaag cctgctcaga
ctacaacctt ctggttttgt 59220cccagcattg ctgtgtttgt ccgtctacat tccttacccc
tgaaaagatt cgttgcaaac 59280ggtgagtggg gtctgtcagg tgagactgcg aagacattag
gaattattag aatatttaga 59340ctacctgttg gctgttccca atggcttttc ctgaatcaga
agagcaggct gtatatgatg 59400ccatgaaaat gctccatatc tctaagtagg tgtagactgt
ttgagaagtt tacaaaagaa 59460tattcttctt gttttccaca tgggttcgta tgctttgctc
tgtttgcttt gtctaagcct 59520tgctagttca aaggacaaga acttaagtct actaattacc
tacttgatct tcagtgtgct 59580catccggttg gaaaaattca ctgactcttg aggcacaata
aagggtattg tggagactct 59640ctaattcctg gtgtgacttt ctcaattgtg ttgctgatgg
tgctttttcc acaacctgat 59700gaacactctg atctcgctaa agcaaagcat cagtctgata
ttgtgtgttc ctcagagaaa 59760catctgttca gaggaaatca tgtcttagtc acggagctgt
gtaacctgcc tggtggagag 59820ctgccatttg tgtagaagta ggaggaagag gctcacaaga
gttttgttcc tttatatttt 59880gtgttatcca agcaagagct ccagtaaggt catgttaaat
gagctagttt ggagggggaa 59940tgccccacat gtgggttctt tcatatcgtt tatctaaact
gaagtgactg cagggtgttc 60000actcacagct catgagctgt agccctagtg cacagcccca
atagcagccc agcttggatg 60060gccaccgccc ggtctgcccc gggtgcgcac tcctcagggc
tctttaacaa aggcaagaat 60120aaaataaata cttgctctgc tttatcagat gatgcttacc
attcagctga cgtgacttgt 60180caggtttcca cacagatgtt gccgttctcc tgattaatgt
tcagaagata aactacattt 60240agcttttctc ttagtaagca aatagcaaac aaagctttgt
ttctgttggt tgcattcagg 60300agtgacaaag caaaaatagt gtcctatact actaaacacc
tttaagttat tttttttctg 60360cactgattct agagcctctc agcttcctcc tgtatctgaa
cgtgtttctt gaactctgtg 60420gccccatcac agctttaagc aaagctgggt ggatcacagg
ctgcatgtgc ttagaaggtg 60480ccaccgtgcc gcgggcctct cagaatgctg acttgttgct
ctcctgggaa agcagggatt 60540cagccagaat ccaagcagcc cttcttgaaa tttcatttcc
aattttgttg actcctccct 60600gtgtgagagt ttcctgtgat tactgactca ggagctgtgt
ctggtttctg ggactgctcg 60660tgggcacctc atgggctttc gtcttgagtg ggggcctcag
cccttctcac tcagccagaa 60720cttgctgcag tggggtcact gacacagctt ggggtgctca
gggctttaaa gaggttcaag 60780acttcgtaat atttcatgca gtaaattcct ttcaagcatg
tgaacgctgt gagctcctat 60840gtgttgtatg tcattaatga atgcagcatt aaaaaagaag
gctgatcaga tgcagttaaa 60900aaagatggtg agatagagat tattctttgc tatccagccc
ttattgaaac agcagggtga 60960aactgagggt gtttttttcc caacaaaatc ctctgaatgt
gcaatatatc agtagcagca 61020ctaaaagaaa gaaagtgata agccttgcca ctaccaggaa
tagattctct tggcataaca 61080aaggcattga gaagcatcat cagctactga gtgaacagga
ggactgtaaa aggttcacca 61140cgaagtacct ccaggtttcc tcactgaaga ggaacacaga
aaccttgcaa aaacgatcca 61200gcttgaatgg taccagaaaa gaatttctac gtcctggtgc
agaattccac tggtgtaagg 61260aagaagagag tcatttaagt ttgcaaaatt tcacaattta
tttccttgct ctgaatattt 61320tgccacccag gagagtgaag cacaggtagc acatgcacat
tttaatatca ctgtaggtca 61380tttgccaata cgactgaaaa tgctgatgtt agaaaggcag
gattgcattt ctggcatgaa 61440gacagaaagg aacgtgaaat gttttgaagt tattatgatt
gcatatattt tcttaggcgg 61500taaggaagat ttggaagtca aaatagcatc agggcagccc
taactgaaga aggatatttt 61560actccgctag caaatgaaat atttttcagg tagactgcac
acatcattct ggcattgtga 61620gattatgcgt gttgtttatc ttcacgagag tggtagatgt
tgaatgacac attcttggtt 61680ccttgggtaa ttttccacgg tctccccagt gagaaatgcc
tgggaagttg gtacttgccc 61740atttcttcca tttttacttc agacagagaa agtatgcata
tggattgtgt gctcgtgggc 61800cttaaagtgc ccttaaagag aatgagttca aagggaaaaa
taaggtaggc atcctgttca 61860gagcagtttg tgtaaggtgc acagaagtgc gtgtctgtgt
tgagcgagtg cagaaaggca 61920ttttaaagga tgatttcaca tgtgctcctt tgacctgttg
ttccaagtga ctccctcagc 61980agcagtccca ggtcttctta tttgttttca ctgtcttttg
ccaccatttt gcccaaagct 62040ccctcctcct ttgatgtatg cggagtccat cgtttctagc
aagcttgact tttctggtta 62100ttagttgctt ttatatgtga gaagttgtga ccacaggagt
gacacaggaa tgatgcttgt 62160agtgctgact ggcactgagt tctcactttt acacccagaa
aaactctgag aacacttccc 62220aaacctcact ctgacaccag cttgattcct gctgacactg
taaaatggga tctcccaggg 62280taagcttcgt taccaagcat cttgggacac tgccagtgtc
aagggagatg gacagaccca 62340ttctgcttga aaagcatctt acagggatcc tttacatgtt
gtaaacatcc ttcttttcat 62400ttttatttgg ggataacttt ctctggtgct gtatatttaa
ttttttttcc tcctcaagat 62460gaattgcttt ctttgcgttc ggaggcaatt aggaaatact
ttgttgctga taccaacagt 62520cagagcactg tgtgagggca cactgctggg taagtgtgtt
tttcaaattt ggatttaaaa 62580agtcttgatt ttatgccatt atcctttttt cacttaatta
gattgtgcat tatatttcag 62640taaccttttg tacagcgtct tttagctaaa attaagccag
gtgccttact aaatatatag 62700aacatatacc tatgtaagtt aatgaaaaca aagacgtgaa
ggccttttct aatcaaacag 62760attttacatg gaaatcaaag ttttctcagc tgtgttgcag
aaaaaaaata cccccctgtt 62820ctgttactcc tataaaaacg tgtgaatacc acagattatt
ttggaaatct ctactctcaa 62880ctaccaaaac tgccacagca tctcgataca ttgatgtctg
atgttcagcc aagtttggac 62940agtatgacac atgctcttga atgcagattt ttgtcattca
aaacaccatt ccaaacaggg 63000atgagagtga gcggtcagaa gcaggtgtcc ttgctctgga
gacagttccc tgcccacatg 63060tcccctcttc cctttcctgt cttctcttac ctaactgctg
tcatctggtg agatctttac 63120tcatctgatg caacctagaa tgcaaaaggt atgaactagg
taaatgttta agactgcagt 63180attaagtagg catttgagag aaatctctgt ccttaaggtg
cttcttggaa gatcagcaaa 63240cctctcaccg aggtaatgct tcagataatg ctacagactt
tcctgtttgc gtcttctgtg 63300tcagagcctg aaacgttatt gcaaatagat gtctggataa
gaacagaact gttaaaatca 63360ccttgccatg ccatataagt tccaatattt tgccattttt
tttcctgggc agggaacatg 63420ttgaagaaag tttttgagtt ctgttggaag tctttccctt
ttgaagtccc ttgcagtatt 63480catcttttcc ttttccttct gtctctttca atagacagag
ctgctgagca ccaatttatc 63540agattgtctt tccccttctt tagggacatg tgattctggg
gatagaagac agtcaaactc 63600actgtgccaa aggagttacc gtcttccata tttgtgctgc
tcttaagctc gatgcgatat 63660tgactgaaat tctgtggttt ccctttgttg tctttaatct
acaccaatgg agttacaccg 63720aagtgcagtt ttagatctat gaaagcagtc tggaagatcg
aatattccgt gtcattccca 63780gaacgtggtc cagaacatct gtcgcttggc accacctttt
ccattcctga ctgcatagat 63840cagctaacag ccctacggca attgcagtta ctctgaactg
ctaggaaaat atttgcagtc 63900atcattgtaa gtgatgagtg ggcacatagc agtatttatg
taggaggcta agtacttaga 63960gtttctagga tgatctcaac ctacaggacc ggacagcttt
ctggagagtt ctagcaaggg 64020taaggagaac agggaatcac ctcttagaga gaggacatgc
cacagctaaa gctttaatga 64080acaattagat gtgaagcaag agacaggaaa gatgattgtg
agacttttaa aagcctatca 64140aagcactagg agagcccaaa gcataggcaa agtaccttat
aagttggcac atctgaagag 64200tatcaattaa aaacatatta aatccatatg ttatccgatg
tgattcaata tgtgtgggtc 64260accctgacca acccagattt ctccacgtat gtctggtaat
actggctcta cgtagcacgc 64320agaactgcca gctgtcactt gaaggtaagg gcttctactg
agccactcgc attaccttgg 64380ttgggcatgg atgagagact cctcaaaagc tgctggtggt
gtctgagact gggcaggatt 64440ggtcaggcct ttctcgcctc ccagcgtagg ttcaagctgc
ccagtcccca aactggtgtc 64500cagcctcctt cagcaaggaa atcagtgacc tgccagcctc
actgcaacag gagctcactc 64560tgtgggtcat ctctatcctt ttctgtttca ggatgacgat
ggatgctctg caactagcaa 64620acactgcctt tgctgttgat atgttcaaaa agctatgcga
gaaggacaga acagccaata 64680ttgtgtttgc cccactgtgt acctccacat ctttggctct
ggcatataaa gctacaaagg 64740gtgacactgc agaccaaatg aaaaaggtga gctgtcggca
tcctgctgtg tagctgcaaa 64800attgtcagag gtggctttcc tatttattcc tcttaatgct
gtataggact gctggttccc 64860ttgtaagcca ggcagaaaac tgtccatcca aaattccaga
atatttcccc actccatggc 64920tccacacaac caaagaggct gaaaatcact agcataggga
aaaaagcttt ctcaagcatt 64980tacaaggtgg atggggacat ggcagagtcc tcagcagttg
tattaaggcc ttgtctcctt 65040tcagcaggaa tgctgattgt ggctgaaggt gactgctgaa
gtcactgcat tttctggata 65100attgtttagt gattattcag gactgcctaa gcttaacagg
actggaaata attttgccat 65160taccaagtaa ttttagcagt tctgtctgtg ccatttcccc
tttctcctgc catacagcta 65220agaggaagat aatgcagtag gaggcagctc agcttgagta
gtagtttgcc ttgcaaatag 65280ctctagatgc tcaagggttt tacagcacca cgaagcagca
tcatggtgat ggtgcaatga 65340gtttatcaag gttgctctgt ggcggtgaga ggctgcacga
ctgcctctgt gagagccagg 65400atttacacag cctcttttta ttccagtgcc cacagtctca
gcagttacct agaggtgaat 65460gagaagcaaa ttcagcatgc atttatatgc tgattatcac
ctggctctca ggggcattcc 65520atgtatttga atacattttt cttcgtttag cagttcctcc
ttgtaccttt ggtttccctg 65580acggcacatt gctggagcac agcctctggc gcctctgctc
atcctacaga ttgcaatgag 65640tctatttgca cagaaacaaa gtggtatatc cacaaaggcc
tgctgggtgt tttcccaaat 65700aggattattt taaaaaaata aaaataaaaa tgatttttag
atcttatttc tagtttaaat 65760gacaccccaa agcttccttg tcatttcaaa gttcaagcac
tgtctttgca atggaagagc 65820ttaaaacatt aacctgtgct taatttcact ttcacttgtg
cctgcaattt gcattgaacc 65880gtcccacaat aagtgaacat ccacatccac aaatagggtt
ctgttacaca agtgcactta 65940tgtttcacat ttctcaaggt aatttactgt gcctgtaaag
acatggtgtg ttcagggaga 66000aagagcagga gtgaggctga aagggaaaag gaggtcactg
atgctggttg ggaaagatga 66060gaagggttgg gcaggctgtt tttaatggaa catgcactct
cagagacctt gcaacaggca 66120ggcacctaaa agcagagagg tttaggtcat gctagaatat
cctggaactg ggcatgtgat 66180ttcccggagc tgggaggtgg gtcagcagcc ttacctctaa
cttacgttct gtctgccaaa 66240gctcacctgc ttatctgact gatttctact gaaataccac
atgacatcat gtgtcaataa 66300tcagaaaacc ttgccatatg gtaagcagtt tttaaagaag
taacccactt ccagaaagga 66360aactaactgg aacatttatt tatctggcct ctaaactcca
gatttttgga caagaatgtg 66420agtttgataa aagcatgact ccacgctgca gatatgtagt
tcactaaatc actttgctag 66480tatgaacagc tctatggaat tctttggact gctcacagga
aggaaacaca tttggttaaa 66540gttttgatag gatcaagttt ttagatttat gtggggatgt
caaataaatt aatttttttt 66600ttagtaataa ataagagtga gaagtcgtgt tgttagcttg
aacacaaaaa agtcaaagct 66660ctggtcacaa acaagcatta tttattgcca agctgtcagg
cctggagcat gtccagagaa 66720ggacaacaaa gctgtgaagg gtctggaaca cagatcttac
aggagagcag ctgaggaaac 66780tgggattgtt cagtttggag aaggagaggc tcaggggaga
ccttatccct ctctacaact 66840gcatgagagg aggctgtggt gagcttgggg ctgacctctt
ctcccaggta gcattaatag 66900aatgagaggc cgtgtcctca agttgcacca gaggaggttc
aggttggata tgaggaaatt 66960tttctttttc tgaaagagca gtgagatatt ggaacaggct
acccagggag ctgttcaaga 67020actgtgtaca tgtggcactg tgggatatgg tttagcgggc
acagtggtgg tgggttgaca 67080gttggactag atcatcccag aggtcatttc caaccttaat
gatactatga tgctatgagt 67140ttttagataa taaaaagaaa ggtgctcagt attttatctt
gttcattatc aggtgctcca 67200tttacaagac gtcaaagatg tttcttttgg gtttcaaacg
gtaactgcag atgtttccaa 67260actcacctct ttctttgcac tgaaaatggt caagcggctc
tttgtagaca agtcgctcag 67320ccctaccaca gtaagtactg cagaaaagtg cttgaattgc
tcgaccaacc agacttcaat 67380gttattcaaa atacgttctc tcactattag cttttacttg
actagactca gatgatgaac 67440agcataataa gagtttgtag gaggatgatt gttctgcttg
accccaagca atgcagccac 67500tgctagagtt gcaattcttt cattaatatg ttttaggtca
gtaggcgcag taggttttga 67560atgcaatatg acttctatgc cacatcaagg gctttgcaat
ataagtatga ctgggaagga 67620ttttaaataa agatggtggt gcaagtgtgt ctagtccaca
cacccaagta attactgcat 67680aaagagtagt tttcttaatc taactgagga ggcacaagcc
tggttattca aacaacacaa 67740gtgaggaaag tgttgtttgg ccatgaaact taaggacctt
gcaaacaact gagaaaaatg 67800ttgtgtttgt tttatcagag ttgcctttga atagggcccc
aagcaagggc aacttcagcc 67860tagaagtgat gtttcagaag actcacagcc tgcttgaatg
gtgttataat caggttgcct 67920gctttttggc cccatccaca gcagtgagca tctcacctga
caaggatagg cacactgtga 67980gcagcctgtg gcctttgtct catccctttc ttttgcccag
gtgtagactg aaggctactt 68040tatcctttca aactcaggca acatgttcac tcctgcagta
cgaaaggtac tttagcagcc 68100agtataactg tattgaagac agtcttggga gcaatctgct
gaatgcggct gcgtgtcctg 68160gctgtcacct gctgttactt attagctgtc ctttgtaata
tactctctgc ctacaccgta 68220atgaagcttg ggatactggt tttgtaggcc gtgtggagag
tcatctagtg aagaacatct 68280aaggaaggtt agctttggta ccttgtgtct ttcaggactt
tgttaactcc acaaagaggc 68340cttttccatc agagctggaa ctagtggagt tcaaggaaaa
aactgaggaa acacggcaga 68400agatcaacaa atctctctca gagctaactg atggtgagta
gggcctaacc tcggggatgc 68460tgattacctc tttgaagaat gatgtctttg tcttcatgac
atctcctaac tattgctttt 68520agaagtaaat atacagtgaa agcaaaggga ctgcacctat
tatttggatt catgaggatt 68580agctgtgtta gcatgtttta aaatcattta ctttactact
gtggcatttc tggaggcaga 68640ccttacatta gcctttggca aagcatctca tttgttttca
ttgggaaagt ttggctcctg 68700gctgcagagc ttcacaaaca tctgacatca atacatcaaa
tcctggcccc gttctctaat 68760ggagagtatg tgctgaactc tgaatttcag gctgttaatt
agtagctcat ctcagcagca 68820cagctgattt tgaccacagg tggacatgtg tttcttactt
ggaaacactc ccgtggcaat 68880agttctgcag cacttttcct gcagtaccac tgagccacta
agtcacaaga agtgcctctc 68940agtgaccatc aaggctccca ggcagaacct gcccagtctg
tgcagggtag aggtctggta 69000cgcagtgccc aaggcagagc tcatgtacat gctgtccata
ggtagctcca gggttggttg 69060ctgcctattg ccctcatgtg gtacacatat gaaaatatgg
gtgcctgagt tacatctgct 69120ccatcccgag gtgacacagg tgcccacagg gaagtacttt
gcgctgcctg tgtgatttgt 69180gcatgaatga agactaacat ccacaacact gtggattcag
tgcctcatga cagtgtttga 69240acagacacaa aataaagcaa gggaaagaat tacgttctct
ttttgaaatc catggcacta 69300tttggttatg aactgtaatt agatggttag cggcatttct
tattcgggtt tattcttatg 69360tatcactcca aaagtgagta gaagctaaac tggaacttcc
cttgaagtct cgctctccaa 69420atgagaaata tttttttcag ttctacctgc tgaatttcgc
tgaagtttca gtaccttctt 69480taaagtacta aagaaaagca gtagacatat tttttattct
gttttatgta aaccgagtaa 69540aaatgtcact tggaagatct gtcttgatcc caaattccat
tttaaacatg gagctgcagc 69600taaggaacta aatgcttcta tttggggatt tccctttata
attaaaactg ctatctgtga 69660ggtgcagggc agaaatattt taattcagta cagtgtttcc
atgttctgtg aaaacagcac 69720atatgttgat aatttactgt attaatgacc agcttaacca
tcttcacagg caaaatggag 69780aatattctga atgaggacag tgtaagtgac cagactcaga
tcctcctagt taatgcagct 69840tattttgtca caaactggat gaagaagttc ccagaagcag
agatcaagga atgtcctttt 69900aaagtcaaca aggtacgtcc tgaaataaaa tagagtacac
cttctactca gatgaatgtt 69960tgccaatttt gtgctaagga aatttcagtg agagcaagtg
aaaaatattt gttactacta 70020tggcattctt agactctctg tcaaaaccta tgtgctgttg
caaaagtacc taagccagtt 70080ttcttgttac gttgctagtt tgaagctgtt ggtgaaacaa
gcactaaagg tcaccgatag 70140taggtaattc tttcctttaa agcacatccc cagtatattg
tattaagtac accttgtcac 70200atgaaaactg ctccccttaa agtaccaaca gctttcacta
gcagtcttac agctgatatc 70260gttacttaca gaagccaaca aattccatga tggtaatcaa
tgtaccactt tcatgcaagc 70320ttgcaaagtt tcctctctca tcttctctgt gaattaaaag
gagtgctaga ttgtctcctc 70380ttgtgttttg cagactgaaa ctaagccagt gcaaatgatg
aatctggaag ctactttttg 70440cctgggttat gtgaaagagt tgaatgttgc aatccttgaa
cttccatgcc ttaacaaaca 70500tataagcatg ctcattctgc ttcccaaaga cattgaagat
gaaacgactg gcctggaaaa 70560ggtgagagaa aaaaacagta ctgagatgat gctttccatg
cacagctgtg tcggttagct 70620gtgggtagct tgggtaggga ctgtcttcct tgaattcctt
cattgggttg ttgagctgat 70680tacatagcaa acgcttgtga agaaccagta atcagagtat
gcacatttag tggagtttct 70740ctggaagtct actctatagg ttaaataatc attatatcaa
tataactgag agtgtaagtt 70800aactctgaat gctacaagca aaagttgtct tttggacttt
gtttttttgg ggtttgatag 70860gactgatgag ttcagaaatg gtctttttgt tcccactttc
tctggactgc acattaattt 70920cctttgttct ttatgtcctc agctggaaaa ggcactcacc
cctgagacat tattacagtg 70980gaccaatccc agcatgatgg ccaacaccaa agtgaatgtg
tttcttccaa agtttagtgt 71040ggaaggcgat tatgacctga agccactcct ggaaagcctc
ggcatgacaa atgtctttaa 71100tgagagtgca tcagatttct ctgagatgtg tgaaaccaaa
ggtgtggttt tgtcaaagat 71160cattcataaa gtctccttgg aagtaaatga acagggtgga
gagtctctag aggtaccagg 71220atatcggatt ctgcaacaca aagatgaatt taaagctgac
catccgttta tctttttgtt 71280taggcacaac aaaactcgca atgtgattct ttcaggcaga
ttctgttccc cataagcaga 71340gaatattaat tatgaaaaag accataaatt tatggtgatg
catgttcctg taaagcttgg 71400tgtcctgact atcacctttg aaaggaattc taagaggttc
atatatcaac agggtaatac 71460aatgtactct acatatgcag cagaactagt ttatttcctt
ttatttaatc cccttaagct 71520gaaggattcc cactgtgcag aacacatgat atttgactaa
gaagtattcc atcctcatcc 71580acgagaatat tttgttcctc tgtgacatct ttttccaaaa
caaaatgaac agagaacctg 71640tttttgaaag actaggagct ggaagaggct ctgggggaaa
gagctgcatt cctgtttcat 71700atccaaaaca ccttcccttg agactcatac tcactgccta
aagggggaaa atgtggacat 71760gtggtgtgat agccctcctc ttgtacttgg ctgtagtctg
gtgatccagg gtgccctgct 71820ggaccctgct aatgcacggt gaaatagtgc agctgaacaa
ctcagagttt gcatctgtga 71880aacagcagct gcaatatgga tgcaagaggc aataataaaa
cacccagaag actcttcagt 71940gtgtgctacc tcagtttgta ggttggggag gttgcactct
actgtgtggg attttttcac 72000tcattctcct tcagacatgg cagaggtgac cagttcactg
cagctgagag gaactctgtt 72060gtatatatcc tgagaaaaag aaggctgtgc agttctagga
tagaaatcac ttggattaat 72120attgaaaatg ccacactctt cagatacaga ttttctgtca
cttctggatt cagcattaag 72180gaggctccac acgtctacct aacctctggg attcaagaaa
gaaataaagg ctttgacttg 72240agtgagatta acactgtaat tagaagtctc caaattccat
atgaaattat ggtaattagt 72300tttcctttct atctaaagac tgcctgctgc atatgttcag
tactgattta cctaattact 72360gttcagaata aagcactaca aaacctgtgt caaatgtctg
tagcacatcc agtgtcagtc 72420tgttctcctt cactcagctt ccaagagggg ataggaacag
aaattggtag attcattcag 72480gacacagtta aaataaatat atgaagaaat taaatctgtg
actgaattgc ccttttggac 72540cacacgataa tagctgacaa ttaaggagta tagtactatt
tggtcaatat atagagtgag 72600ttcaattata tatcttcaaa gaaggggcca ttttaactga
gtattcccct tggttcttca 72660gatctgaaag aacctagaga tttctaaatg ggaacataca
acccttaata catattcctt 72720tcttctcata gcagagaaca gcacagtggc tattatggat
ttggagagag ttctgttttg 72780attcttggcg ttcccaaccc acatgtagcc ttcagtcaca
aacgttcagg gtttcaccag 72840ttgtcttccc tctgtgagtc tctaatgctc tctgacttat
cttaatatca gacagttagt 72900ggatacattg gttcacatct ttgaaggcac tgaggggact
aactcaccaa aaccaacaag 72960agatgagtcc ctgcaggcat ctgggggtct ctgctgctct
tcatcaggca ctctgataca 73020tagatagaag aatgctatgt gtaagactta atttcatact
gtctgagagg agacagcctg 73080caaagacctt tactagctca atagcttcag tgataaatga
gtgtctggaa aatgttttaa 73140tgtgtcccat cattgtctga tctgttttgg aggctgcggt
tggagttatt cagagctgtc 73200atcactgcgg tgtcccctgg ttctcccatt ggtctgggca
ttgcacgtgg ggttggcagc 73260cgatgagcag ctgggcagtc tgtacatagg caaggtggac
tggttgccta cagtctgtgc 73320agtttctctg tgtttcagca ctgactcgtg gtctagtaat
tgcaggtatc cagcaagcca 73380aatcacactt cattgctact gctgtgctgg tctgctagac
tgatgaaaat ctctgttaga 73440ctgcctcatc tctctttttc tgcctggata agacttatta
aaggagaaaa gctgatatat 73500cactctcaga ttttctagat cacgaaaaca ttgcagtgca
ggagccattc aatccagctc 73560atactccatt taaatgctga tcagaacact tagtgcatcc
acgtacgttc ccagagagct 73620ccttgttggt gcctttgcca aggagggcta tcccatgaaa
gcacacagga atgctgcctc 73680cggcagagac ctgtctgtct ccaccttctg attaacacaa
aggtacccaa gactatgcaa 73740ggcaagctga tatttcagat aagaagttgc ctcagtttac
agaaggccat gaaaagctct 73800ggttcatttt ccatatctgc ttcttctctc tgcttttgga
aaataattct tatttcctac 73860tagcaagtcc agactagtgt aatatttagt cttgatttgt
taaatcatcg tgaattttag 73920cttttactaa tggtatctta gatgatctaa tatactaaca
cctagtaagt gacttcagat 73980aaggtgttag gcttatacca cagcccagtt tgaaggagct
aagtgcagat ggcaaccaaa 74040caagcaacaa cacaaatagg cccaaatagc ccatggaggg
ccagaaagta cagctgcagg 74100cagagctgtg atgtaatcca tttttgtgag ccccttgaag
ccagcaggcc agcctcgtgc 74160ttttagtgta atggacatca tacagccaaa aaggaaggtt
atgccataat ccttgctcca 74220ttcagcaatt ggactcgagt gtactagccc tgtttcctag
acccttccag tcctatggaa 74280atttagaagt cccaatgtaa aacctattca acttgcagca
tgtgagagga agatatcaga 74340acagctcccg ctaacgtgag aaatgccatt acagggtact
gtactgttcc acccattctg 74400gcctctgggc tgtgggcaag aaactgcatg ggaggacatg
ggaaaacctg tgtgggagct 74460tgcatataga aatgtatttg tactcaaagg cgttggctgt
gacggagaaa gtgagcatag 74520gtgaggactt gctcagatca ccaagcaacc ccacctcacc
tttcaacaca gcattactat 74580ccaatgatgg gtaactggcc tgatggaaaa aagcaagcca
tggctggctt ttgcaggcag 74640ttgcaaactg cagtgttatg cagtctagcc atctcataac
ttgcttagct gcatagtcat 74700tgcccacttc ctgctcccag cattttgtag aagagaactg
gtacatctaa atgctttgca 74760gtagcaggaa ttggttttgg agatgagcag ctggttttga
aacttgaaaa atgccacata 74820caggcaattg gcctggctgg aaaagcagtg cggttttgag
ccttaggtat gttacatgtg 74880caagtgtaga gtcctacacc tggggaggaa tgactgcagg
taccagtaca ggttaggggc 74940tgagctgctg gtgaggagct ctgtggaaaa gaacctcggt
gttctggcgg gcaacaggtt 75000ggccatgagc cagcagtgta cctttgtggc ccagaaggcc
aatggtatcc tggggtgcat 75060taagaagaat gtggtcagca ggttgaggaa ggtgatcctc
cctctctgtt ctggtgaagc 75120cacatctgga gtactgtgtc catttctggg ctcctcagtt
caaaaaagtc agggagctac 75180tggagagaga gtccagcaga gggccacaaa gatgcctggg
gtcctgtagc atctcccttg 75240tgagaaaaga ctgagaaacc ttgggttttc cagactggag
aagataaggc tgaaggggga 75300tcatatcagt gattacaaat acttaaaggg cagaagccaa
gtgaataggg ccaggctcct 75360tttggtatcc tgtgacagga aatgggcgaa aattcaacac
caacaagagg aagtacttct 75420ctactttgag ggtaacagag gactggaaca ggctgcccgg
agaggttgtg gagtctcctt 75480ctctggaaat attcaaaacc tgcctggatg ctttcctgtg
caacctactc tagggagctt 75540ctgtaggagt gagttgaact cagtaatctc cagaggtccc
ttccaacctc tacaattcta 75600tgattccatc ctaacggcct tagaagggtc agaatttgca
catacggtat aatgttctga 75660ggcaaagcag tgaaacagat tgcaaagaag ctctaaggag
atatagagag caaatcaaag 75720aaatgagtgg aggaacgacg tcagtgtaaa agggagggag
aaagcacaga gtttggaaga 75780acgaaagcag tgaaaggtat tcaaaaatgg cactgaaaag
tggctaggac tcacaaaagc 75840agcagaagaa aaaatggaga atggaatggg aaaggcaata
ggcagaaaga aagaaaaaga 75900taaagaggca ggaacaaatg actaagaagt ctgaagaaat
atgcagaaag gaaaagcaaa 75960caacaaagcg aatccaaatg gaacagaaaa aaagtgaaaa
gaaggaaaat attactgagg 76020agatctgata ctgtgtgcag atttgtgctt cccatctctt
tctttgcatc tcactgcatg 76080ttggggacat aaccccatgt gatcagcctc tcttggactc
ctattcttag ctccagtgat 76140ttaaaagaat accccctggg tggggattcc tgttacagat
caaggaaata cttcttcctc 76200tctctaatta aaatccttcc actcacagaa agctggctct
gtacctgaat gcgtgcctac 76260tacctgtagg caacataaag ctcatgcatt tcctattcat
ttgctctatt tctgcaagca 76320agctcagccc caaacaaggg atctctaaat cctagcaaga
accctgcaca ccccagtgtt 76380caagtcctga caccaccaaa ttcaaaagga actacacaca
gcaccacagc catggatctc 76440cagtgttaat gctgttctcc agctaagggc gacttggctt
tgcagtcagg agatgttgcc 76500aggatgcctc ctgtcaaact agttgggcag ctttgagcga
aatgctgtta gcacattgct 76560agatataggt ttcctggtct tctgcaggaa actgaaggat
gacatttgca tgaaattaca 76620acgtgcagcc tttatcaaca attggctaga gactgaattt
tcccacaaga aagtggaaga 76680aatttaaaat agagtataca caggaaggtg ctccagagct
cagctgttgt gttcttcatt 76740tgacctcctt gctcaagaag gtaaacatta tttttctctt
caaaaataac ttgtcttgtt 76800gttgttgttg ttttggccaa atcagtctaa aagttggtaa
atttcatgtt tatagatggg 76860gcaaaggggg aagtactttc acaggctgga gagagcaaaa
gacactgcta aaatttgggt 76920ggtcttcacg aagggaggtg gtcttctctg gggtgaggct
gggaatttag gaacacatgc 76980ccaaagctat gaatctaaag atgcctgtct aaattcctca
gacttttgac tgaaatttcc 77040ctcggttctc cctgcctgct tggagagcta taactgccac
agactgagtg gtttatacca 77100catgcagatg ctttgctgcc tacatctcca gaagggtcaa
agggctgttt tagaacagcc 77160caactcactc taaaaaaatg ggctttatga gaagcgatgg
tgcagatcat ctggataaac 77220tcacccataa attaatagaa acaggttaat tttccttctt
tactcaggtt tccacagcac 77280aaggaaaagc cttgaaatgt tcactagaca agagagggca
cgcaactctt tggttgcgtg 77340cttgggtgtt tcctctgtac cctggtctct gctgctagga
ttgtttatgt tcttaaacaa 77400tggctgtata taataggaag ggtggagtat tcttcagatt
ttgttttggt aatggggatg 77460cttcactatc aacatatttg ctcctggctt tggcagcggt
gttcaaaaat ttgctgagaa 77520gtttatgtaa ctacacattg gcataacaaa tagcttgcca
ctgtatggcc aatgtacatc 77580cattcctgtt caagcaggaa taatcagcct agaaagaagc
aggaaagata catcctggag 77640gtaccgatgc aaaataatag aaccagtcag gaaaagccct
ttcctgtata aaaacagcat 77700catgaggggc taagttgctt gggagatgga cttggcaaca
cttctcctga aaagatattt 77760tgtgctgaaa tgtattgggt tttaatttaa agcacattgc
tttggaaatg ctttgtgttg 77820catggggaag actcgaattt ctgcgttaaa ggaaatttca
tttttcttat gtgttgtgtc 77880cctttaaacc caaaaagcca cagaaacact ttgaaagttt
ttgtatgaat ggtcatgaaa 77940aataacttct acaaccatag gcttttcatg tgaggacact
gtattatctg ttgtgttctc 78000cttttctagg atagacacgt atcatttccg ccaattctct
cttcctttgc ttatgagaaa 78060taaatgtata ttaaagcact taaatgagaa gaagagtaag
tatgcaattg gaattatcat 78120gcagcatcag ggaaaacagg tttcttcttg ctttcccttt
ctacatatag aactgcctta 78180caaaccaggc taccactctt tcagaatctg cattttattt
actgcctcct cctgttgact 78240gcataatgta acataccaca tcttttaatt atgatagctt
tgagcgcagc ttttcattct 78300tcagtaagtt ttgccttgat ttcatcttta gcctaaaaca
agctctacag agagaacaga 78360gcgtgaacag ctatagaaaa ggagtatttt tcacttcacg
gagccatgga agcaatttgt 78420tatccttaca agacttctgg tatacagtgg tatctacgga
aggaggctct tttcctgggt 78480agatccctgc tcacatataa caacgccagc aattccacct
cccagactgt taacagctac 78540tgagccatca tgcaaagcat ctcccttcat cagccataaa
accacagccc tgcttgctgc 78600ctctgcacaa ttgctaatgt tctgtgcaaa cagtttgtct
ggtgcagaac aacagctgtg 78660atctttctgg aacacttctt ttgatcttgt attttctctc
cttcccactc caagatcttt 78720taaaagaacc atttccattt gttgcccaac atctaggtgt
tctcaaagtt actctgccct 78780cacggtggct ccaaaactca ccaacaaatg attacagaga
tcataagcat ggcttaatga 78840tgtggaatca tacctacaca tactccctct ccaaatatcc
attaagaaag ttcactaaat 78900cctttggttc tctagtaaga aagttccttc tccagcccac
atcccttctc cctccactgt 78960tgcattgctt ttctggggca gccctgtaaa tagctcacat
gaagccatgg aattggtggc 79020agtggttgta cctggacgtc actctgaaga cagtctgctg
ctttttctaa aggcatggac 79080acctctgtac gccagacgct tgcctttaag acctgtttcc
agctctcatg ctctccctct 79140gtgcttggtg gttggttctt tccctgtggg ttggggtgga
ggtgcctctc ttctgttgag 79200gaagttcatt agctcctgtt gtctcctcga cgccttctga
ggtctagaca cacctacaac 79260atgcatcctg acctacattc acagtaaaca acctcttaga
tccattttag atcttttacc 79320agctgtgaaa gtggagcaac acaaacttta acatgaaaga
agtgctgagt tttgttttca 79380gaaggttgtg aataatagct aacgagggtg gaagaaaaga
gaaatgatta ctgcaatgtg 79440tttttcttgt ggtaggatga ctgcccattt atgttaggcc
ttcatatgaa gtactactgg 79500acttcagggt gaaacaagtg tcttagaatg aaacatatat
gaacttttta tttcaagtta 79560ggtaaaagga aataaatgcc tgcacttgcc acatatcagc
accttcatat gttcagcaac 79620ttgactttcc tgtcaatcta tcttaggcta agcctttttt
cttgtgggct gagttcattc 79680ccattgtctg ggacttgctg caagctaagc tgctcgcaca
gacaacttgc tgcacctcag 79740cagagccata gcaacttctt acaccctgtt aactttggtg
cctgagcccc cacttgtcat 79800acaaagatcc tgcctgtctc acacctgaat gagaggcagt
gtgtgttccg catccttgca 79860gtcagtgcag gacgctgagt agttcttgtc ccagagcagg
ctgaaagcta gagccaccct 79920gacctgagtg ctttctctcc acactgtgct atatattttc
ccctaaataa aatatctttc 79980tggaacacag gccacagtta cttatgtctg caagcagcca
agagcatatg ctttgctttt 80040cttacatatt tctggtgtgc tgtccagaac atcctttgtt
tgacactaaa attgatgtgt 80100gctttttatg gtacaatatt ttgagaaaaa cttgagtact
ccactgctat ccacacaaca 80160gctttacagt tatttcccta aaggactgat aagggcttct
taaaagcctt tttttttttt 80220ttcagatggc attcttcatg aaaagaccaa gctgaaactt
agtcccaaat tcttcttacc 80280agagtggatt taatggccca taggaaaggc atcagactgc
tgtatttaca gtacaagaga 80340aaagaatgag acagatcttg tcctgccatt gaacaggaag
cttacagact ttctggggct 80400gctgagctat tgcttcgttg tgaaattgcc attcgttatc
cattctgaat cagtggttcc 80460tatcaaatca atgaggagac atgaagtata ctgcaaacag
tgcatgtttc cataggtagt 80520agcattcata gctgcttacg ttccttcttc atacatgaaa
ataattacta gtaattttac 80580tttcatgaat ctgttgtttg aatccttcac actgcagctc
aggttaccag atgtggttag 80640atgcccgtgt agtttctgtc accccaatct gtctctaatc
atgttgttac aagaggaaag 80700aactgatgcg atgacacaca ttaaactagt ttgtagaagg
aaatccacgg ctgactgatt 80760taaataccac aaccttttgc ttacaaataa gaacaagaca
gacagaccac gggaaactct 80820tttggaaggg atcagataca ttgtgggata agatggaaaa
acaattctct ctaaggaatt 80880ctcatatggt atgagtattg gggccccttt ccagatcctg
ctgtattcac atgagtgtga 80940attaatagat gtgtgcaaaa tcagctattt caaactcaga
attcagcaca cttctactat 81000ttagcaaccg actatgggat gattttaggg cggacagata
cttcacagta tgatacagat 81060aagcaatcag ctgattcaca tttctccttt ccctttttgc
tcccagtaag ctgcaggctt 81120cacaatgggc tccatttcta gaatgattat tgagttttgc
cttgatctct acaataaact 81180caacagaaca gcaaaaggcc aaaacattgt cttctctcca
atgagcatct ctacctccct 81240tggcctgatc cttctagggg cacgaaacaa cactgctgct
cagatagaag aagtaagtac 81300tgctgaaatg ttctgagata cttccacata gcctgctgtt
cccccagtgg caatgctggg 81360ctttgcagca caacatgtgt gcttaggaga caaagataaa
cacaagctca actgctgcct 81420tgagagcagt gcttggtgtg ctgtgatccc tgctcactta
tcaactgtga cattcaaacg 81480attcaacatg tctcacctac agagcacacg gagcctgggg
gtacagggtg ggcatgcaga 81540agtctgttcc tctggtcacc atgcctttta ctccctgcag
tgcaagctgt atgctctgag 81600atcttttatt tcttttctta tttgtttctg agagcagtaa
gtgaccaata ctcctaaggt 81660atatgtggca taaggcagta gctggctctg gctgtgtcct
ggtggatctt catccattgt 81720attataatat tgccacaggt cagctgctgc caagggaaac
tcattctcct tatgaggttc 81780tgagtgactc ttgcttagtt taggaaagca atggagatcg
agtactctca acaaggggga 81840atggcgtcta actaaaagag cggaaattta ggtaagatgt
taggcatata ttctttacac 81900agagggcaat gaggcaccag cacaggcttc ccagagaagc
tgtggtgcgc catccctgga 81960ggcgctcaaa gccaggttgg atggggccct gggcaacctg
acctggtggt ggcatccctg 82020cccacagcat ggggttgggg ctgagtgggc tttgaggtcc
cttccaaccc aaacctttct 82080atgacagtta ataaatctac atcacttatc caggacagcc
cagtaaatct ttcaaacaag 82140gaaaatgcct ttatcccagt taaaattgcc attaatttga
cctcttcaac tgcaggttct 82200ccacgtcagc aatgccgcag gaactacaag ccttgaatct
gagcttgaag gtgcagtgcc 82260cgaaaacaag tctgaactaa gccaggaaag agagtcttcc
ccctctctgg tatgtctttt 82320ttagtacaag agtctttcac tccacagtag cctattagtt
gtaaagcacc acagcctgcc 82380acaggaggga gtcaagatcc catgcacaac gtctgcctgg
tctactacgc ctgattgaag 82440gtgttccctt gtaatcagcc aagtcctcca taaagtcaaa
tacaaagccc ccaccagaag 82500gaagatcagg ttacaaaact tagattagct gaatttaaat
ataattacag tgggagctag 82560ccctacactg caatctaatg aggatgcaaa tgaacaacca
aagctatact gaggaatact 82620tgtaattggt gtgtttgaaa tattcctagt gcaacacaga
tgggaatctt aaccacgaag 82680cgttccatgc actgctttta caactacaaa accttggcaa
agactatgtt ttaagcctgg 82740ctaacagcct ctttatccaa caaggatttg aaccgcatca
ggtaagataa ctgtaccttg 82800taacctctgt ggcgctgacc cccagctttc tggcaaccat
atgcttcact gttgtccctc 82860catgtgtatt tttgagcatt ggaggtgctt cttggagcca
tatctcttag ggttgttggg 82920aaagagacag aagtatcagc tttcagtgct tctgtttaaa
acaaacaaac aaacaaagtc 82980aagacaacac tctgtagagc aaaaataaag cagaagacct
ttgacttttg gcatatctaa 83040cttgagccag aagtgcgact acagcaaaaa aatggcctat
tcaagctgtc tgcaagctgc 83100ttctgggcta tctttctatt tgcagctttg cattgctggc
tttcctcttt ttcttctttc 83160tttctttttt tttttttttc cccctgctga atgatttgga
tacttgagaa tcacccaaca 83220catcttgcat cttctctaat ttttttttct tttctatttt
tttaaatttt tatctggata 83280cctgcatact tcaggtatgc agttttctgt gggaagacat
tgtcatctag aggcaaaaat 83340gtatataaat aataagaaag acacaataat aatctctttt
tcaaagatta tctgaatcag 83400cttctgatag ttgatgtttc caaagccaaa ttttgtctct
ttcagtcaag aagaccctca 83460gaatttctaa aacgtttctg aattgttgac ttcatgttaa
agagaataag ctctgaacag 83520gtttggctaa ttcacaatct ttattctgct ttacagaaat
atctaatgtg cagtaaggaa 83580ctatacagag cagcccttga aacagtggac ttccaaaggg
ctcttgaagc aagcaggcta 83640aaaattaatg attgggttga aagcgagaca caaggtaaaa
cagagcaaaa ctgtagctgt 83700gctatcttct ccctcttcca gtgctccttc aaaaagaatt
cagcatatga taagtcttgt 83760tcatgtttct aggtttctca tgcccgtcaa agatagtttg
ttgttcccaa tcattcttta 83820gagtcatcta ccagctaaac tatttctgag ttaaagatgt
gtttgttgtc acatactgtc 83880atactcctac ccacatgcct agcaagataa ctgcaacagt
acctctaagg gttaaataga 83940ttaattgctc ctgcaaatag ccaacactgc aggtacagta
aagcagagga cggaagttat 84000gagcgtcaca gtgagactgg gaacagcata gcagagagag
aagacacctg aggacctggt 84060gttgacctgc tctggtcgta cacagagcaa tgctaacaaa
gatgagtgat gtgcccacca 84120gagagatttc actgttacaa gtaacaacca accagctttt
gccctttaca ggcacataga 84180ggtcattggc tttttttctg attaagctga acatgaaata
tgccactttt attttgtcag 84240agatgcaaca tcagcagggt gaaaacctta taaatcttcc
agctgaactt aagccagaac 84300ttactgaggg aaattactga tggatgaata gatttgaagg
cttctgattt cttaatggtc 84360atatcctgac caaacctgtc cttgggctga cagagcagcc
tgtgactaat gtgggaaaga 84420gctgcaaacc ccagaccatc attgctctgt gtgcctgtac
aaagcctgcg cgcttgggaa 84480atcctacttc acctctgtac agaaaaaaaa agggtaaagg
gaaagatgcc ctcatgtaaa 84540ctgaaacaga ggattaatgg cgctgcgcct tttactgtgg
acaggtgcca cctggaacat 84600tcattttgcc actgatccca cagtaggcta atttgatgat
cggtgcccct tcctctccct 84660aacaggccag tactaggtaa cagtgctgag aaatttacca
tttctttgct tgtatcgtcc 84720ctgttctgtg aagaaacaaa cagttggatt tctaaggtac
tctaaagcta agttcacaga 84780caagtaattg agtctcaatc cagagcctta ataacaacta
ataaacacct gtgttttcca 84840aaatttcctc caggtaaaat caaggaactt tttgctccag
gagtgattga ctcacacacc 84900attctggtgc tggtgaacgt gatctacttc aaagcatcct
gggaacacaa gtttgaggag 84960aaaaatacag tacagagaga ttttaaactg aatcaggtag
atatgcattg tataaatctt 85020agcatgattt acctgagtta gcatgattta catgagttgc
aacgactcag cattttgttt 85080caatggctga caaaacacaa agcttcagcc ctgatcagcg
cttttgaacc taatagtcac 85140tatgggcagc tgtcatggat agaagccaat tgcaaagatc
tcatttcaca caggctctgt 85200ggggccatcc tggcttttat gcatcccgta caattcagcg
tgagccatgc aacagatagg 85260ttaaaccaaa ccaatcaaaa aaagaggcca gatattaaca
agccacatat atgaagatgg 85320aatttgaaac aggaaaaatc ctcacagagt gttttggttt
atttatagta tctgcaatgt 85380ttaaaaggtt ttttttaaaa tatttttttt attttgattc
cttttttcca ccgtacatat 85440aaaatggaag ttttcattgc tcaactaagg tacagaatca
tagaattact caggttggaa 85500aggacctcaa agatcatcaa gtccaaccgc agcctaacca
tagtacccta actctaacaa 85560ccatctgtta aatcatatct ctgagcacca catccaaacg
gctcttaaac acatccaggg 85620atggtaactc aaccacctcc ctggggagcc tatcccagcg
cttaacaacc ctttctgtaa 85680agaagtgttt cctaacgtcc aacctaaact taccttggca
caacttgagg ccatttcccc 85740tcgtcttgtc acctgttgcc agtgagaaga gacctacccc
gctctcactg taagcacctt 85800tcaggtactg gaagaaaata ataaggtctt ctctcagcct
cctcttctcc agactaaaaa 85860gccccagctc cctcagcttc tcctcgtagg actgattttc
caagcccttc actagccttg 85920tgaagctgca aaaagttctt taacaaccac attaatccaa
gctctgtaca gctcaagtct 85980aacaaatgtc ttcaaaaaag atgatcaaaa ccattttatt
tcatttaatt cagttttgtc 86040ttcattccat atgctgtgcc tatgttacac taaataatga
agccgccaaa aaaatgaacc 86100cacaaaaaac acagatttag ctctgatctg aagttgaaga
gctttgtatg ggaaaaactg 86160tattctaagt gtttcttatc tatacaaaca aaaggtcaga
aagacatctg ttgctagccg 86220tagtgttgca ctgccattta ttaagacacg taagaaagtg
taattttggt cccttaattt 86280ttttacttga aatatgtctt tgaatttgaa tactgaaaac
tgaccttagg taggaacatt 86340tggaacactg ctgcagtcac agaaactatg agattggggg
aatctgcata tacttttctt 86400catgcactaa ttaataatgt tctctactaa aattcttccg
ctgatttaga aggtaagtaa 86460aaacttagct aatggtgaaa tgaaccttga gcctttacac
aggatttgaa caaactcatc 86520acaaaagaaa atgaggctta gaagacctag aagaacatgc
ctgagattgc tcttaatctg 86580tctattgctt cctgcctaaa acatctacct gataaatgac
aacctgattc ctgcagtgct 86640atttcttctc tatcccattc caaaccagga cttgcaaatc
ccatcagcat cagcttgttt 86700ggctggagag taatggtatt aagccacttc actatctgat
cagttgcagg gaaattgctt 86760tgttttattt tgccccccag agaattatct cctttataca
tgaatggcaa aactgatgtt 86820ttacgtgtcg ttgtatgtgc aacaaaataa agaaaaaatg
tttagcttta taacaattac 86880tgctgcaaac acagactact gatattgcac ctgaagttta
aacattaagg tctgtattgc 86940ttgtgtgatc attccaattt ctttttaaat agaatgagag
aaagccagta cagatgatgt 87000atcagaaagg cacatttaaa ctaggctata ttgaagagct
gggaactcag gtgcttgaac 87060tcccttacgc tcagaagttg cttagcatga tcatcctgca
ccaggagaga cagcagatgg 87120atctcccagt ggggctggaa caggtaaggg tgaggactgc
ggctaagccg gactgaaagc 87180tggttgtctg aattaaagct gggcaaaaat ctaaacttgt
taatttcccc atcttctaga 87240ctgaaagcac aatgacctat gaaaatttaa tgctgtggtt
ctcttccgaa catatgtttg 87300agatggtggt agaggtgtac ctgccccgat tcaagctcga
aggcaccttt gacctcaatg 87360aggtattaaa agcaatggga atgactgaca tcttcagtga
atccaaagct gatctttctg 87420cattgtcatc tgagaaatcc ctggtgttgt caaacattgt
ccacaaggct tatgtggaag 87480tcaatgagga gggtactaca gcagcagctg ctacaggagc
taccattgtg aggaggtctc 87540ttcccctcat agaggtgttc atagctgacc gtcctttctt
attctttatt aggcacaatc 87600ccaccagtac cattcttttc tttggtaaat tctgctcacc
ttaaaatcaa ggccatcttc 87660tagcattgtg agaaaaacct ggatgaatca gaaatactat
ttttccccct acaccttctt 87720attcctatga atgattgtag atcaaagtaa tcactgcagc
caacctagcc tagaaccatc 87780aattgaatgc cctcctgtta tgctccttga atggcaaata
ttgatctgaa tctaaaacag 87840gagtaagttt tcccttaacc tgactggaaa tcaagaatat
tttgtttctt caaggcgtac 87900atacactcct gtatagccaa gtatgtccgg catagccaag
taatgtagta cactatttgc 87960ctggcaaagg tagaatttgt atgctgctac ctgaggagaa
ctgtttgtaa caattttcag 88020taactgccag taaaagtgga gtatttttat tttctctgta
gtttttgatt tcctgccagg 88080tgggacttga ttaacagaga ggggctttgg aaatgcttta
tacttataca taatctgtat 88140ttgtggcaaa tccttcgcac agtggagatc tcactttgat
aattcccttt cctgtagcag 88200cagtcacaag caagcaggaa atacttattt acagcaaatt
cacgtgttta ctgacaactg 88260taccaccttt ccccccatga tgtatgctgg atctatcctt
ttgccatata aaacgtttat 88320gctagaagca gctttggttt catttattta tttagatata
agcctgcatc tgaagcacca 88380actcatcaac tggaagatag atggaatatg acatataccc
ctttcacaat cccttggttt 88440tttccacatg agttctgtta gaagcactgt atttttcctt
ttttaagata acaacagtag 88500gaacactcat ggaaaggaca agattacgcc tcatgaacac
atctagtaag agagttgatt 88560ataacagcaa ctgagtatgt gggaaggcaa gattttgacc
ctcgttttac aggatttttt 88620ggcactcttt tttgaaaata aatccaccct taaagaatca
cagcatggtt gatgttgcaa 88680gggacctctg gaggacattt tgtccaactg tcctgttcag
gcagggcaac catgtccagg 88740gggcttttga gaatccccaa gcacagaaac ttcacaacct
ctctggacaa cctcttctga 88800gttcccacaa ttttgaatga caccaaagag aattttgtat
gcgcagtgtc tgcaggaatg 88860ggatgtgaaa acacacattt ctaaagctta attacttaca
tagtgaagta attggttttc 88920ttccttgagt tctgctctct ggtgaagttt aatgatctga
gatgcatgta tatagatata 88980caggtctctc cagccctgag gaatgaagaa aagttttgaa
aagggcaatg taagcaatag 89040aaatcacagt caaatattac ctggaaaact ttttagtctg
agagataatt agaaaaatag 89100aattagcagc tgactgatag agagacataa ctgttaagtt
gctggtttaa cacaagtaat 89160atcttcctca cagagttcta tgtgaggttt aactaactag
cgttggcaac ttgtgctttg 89220tgacctataa aaaggcaagt atacattagc tattagtcat
ataattgagt gtaaagctcc 89280ataaagtaat tcatgattag cacagtttat gtaccaaaag
ttacctgcgg ctctttggat 89340aagaaagtct aggcatgatg ttcgagcaag aacaggcagg
agtaggacaa taatattcaa 89400acaacttacc cttactgact aatctgaaag cacagtacaa
tgtaagcagt acttttccag 89460attgtgtcca tgtttccatt ctggaggctg acagcacaga
ttgcctacta agctatgttt 89520ttattacctc caggtgtcat cacttggttt ttacataccc
tggggaagtt ctgagcacca 89580caacctcaaa catcagtccc acttctgcaa cgacaggaac
agagattcct gtgatgaagc 89640gtcgaataac acagtgtctt gctccagttg ttggaggaga
tggttcatga taaatctaga 89700gtgagattaa gacacagatg aggtcaaatg tcatccagct
agtttatgac aaattctaag 89760cagttaagga atgtgggaaa catggcaaag ttagcaacag
taaagggagg aattctagca 89820aactggctat agagcaggga tactcacccc catggatcta
gcagtatccc attggtttgc 89880aggaggttgc aggtcagtca aagacatatc actgatctgc
acagctgcag ttcagtggag 89940gattgtctct gttctaccac tgaactcttc aggctttatc
ctcttcattc tgctctcatg 90000caccttcagt tactcagggc caatggcatg tgtgcctccc
attgggtgat cggctgttga 90060tcatgcagca atcacacacc tgccacctgg cacgctgttc
ggcatgtgta ctgacttaat 90120ggaagagacc ttttaagctc atctagtcca actcccctcc
actgaagagg gacacctaca 90180gctagatcag gttattcaga gccccgtcca gcctcctcaa
tgtctccagg gaaggggctt 90240ctaccatatc tctaagcagc acattccagt gccccaccat
cctcactgta aaagaatttt 90300tctttatatc caagccaaat ctcccttcct ttagtttgaa
actatttccc cttgtcccat 90360tacaacagat cctactaaag aatctgtctc cttcttctta
agagctccct tgagaaggga 90420gctcttctca ggtcaccttg gagccttctc atatccagac
tgagcagtgc tagttctcag 90480cccgtccttg taggggaagc attccatccc ttggattatt
ttcctctgga ctcacttcaa 90540cgtccatgtc tcctctgtac tgaggactgc acatttggat
gtagtactct aggagaggcc 90600tcaccagcat agagcaaggg acaggatcac ctgccttgcc
ctgctggcca tgcttctttt 90660gctgcaacct aagatacggt tgactttcta ggctgcaagg
gcacactact gactcacgtc 90720cagatgccat ctaccacagt acccctaaat ccttttctgg
cagggctatg ctccctcttt 90780tcgtattcca gcttgtaaat gtagtggggg ttgccataac
ccaggtgcaa gaccttacct 90840ttggatttgt tgaccctcat gaagttctct cgggcccact
gcttgagcct gtatggatcc 90900ctctgaatgg catctcatcc ttcaggagca tccactacac
catacagctt ggtgtccttt 90960gcaaacttgc tgagggtgca tcaaaatcct gttgacaatg
ttactgatga agacactaaa 91020gagtactgat cccagtactg atccctaagg aacactactg
gtcactgatc tccatccaga 91080cattgagcca ttgaccacca ctctctgggt ttgatcccgc
agccagtttc tagtccacta 91140gtcagcacac cactgatcat agccacactc gaaggggcag
tcatgcaagc accaccctgg 91200gtatttattt cccagcactc taaagcagag ctcttgctcc
agctcatgtt attttctgtg 91260tggcaaggag tgagattcat cgactctagc aaatggaact
aatggctcca tgtgccccag 91320gtctcagctc agcaccagcc aggccagggc tgagtccccc
cacatccaac ccataaggtc 91380ccagaggact cctacgttta ccagtggtgc acagagatga
gtttagccca agtccacccc 91440tcagcctcaa ctcccttcaa cacctcttca ccaagaggcc
caatccatca ctccttacca 91500gccaaaacat atacttgttt aataccacag ccacaaaagc
cacgtggtaa ggtctgaaga 91560gaccaaaact gtggtttgag taaaacagaa ggaaagcctc
tactcagtac cccacttatg 91620actgagttac taggatagga cctgattcta cagcacccca
ataccctgta gatgtattcc 91680tttaattctt cacaccagat taaggctgct gccaccaccc
accacaaata aatccttgct 91740taggctgatt ataacttaca cctgtggctt ccacagtcaa
atgagattcc cagtgcccac 91800ctgcgtgttc aacttcctta aggcaaagca tcttgcagtt
agcagagtgt taagaaatct 91860tcttgtattt cctttaacac acgtttatct tccccagtga
tgctgaattt gcaaatgctt 91920tagggaaaaa ttggcagcaa gtccttacat aattactgtt
tagcctagaa aataacaacc 91980gaggtagaat acttcagaaa gtttctaatt taaggttttt
ttcttgatga gagaaaagtg 92040ctatcagagc tgtttagtaa ttccagtcat gcatgggtaa
ctcattcttc tgtgttaggg 92100tttactgaga ggtgaagaaa caagtagttt cttttcctta
tgaaaaaaaa aaaaagtggt 92160attagaagaa ccccataaaa gaatgccaaa cattgcagct
tatgatgtgc aatgtgtcac 92220tcagtcttac agatgacaca gcctggaagt aagcttaaaa
aaaatgttta attcctaact 92280tcttttgaca ccatctgtgc tgtggtttat gacatccatt
aataatgttt atcactaaac 92340aacaacagat agagagacca gaaactaagg atgctgctgt
catttccttc tgatgcaagg 92400tagaaacatc aggaaattaa ggcacactga aatattttgt
aatattttgg actagaagca 92460aaaccagaaa ctgagttgca tttgtctcct ggagtacatt
ctacaggtat ttaaaaagag 92520acaaaaacca taaatctact tgaatttaat ttgaagtatc
aaatgaaaaa gatgtacctg 92580attttattat cctccacact ggtcttctga acttgaccaa
tcccactggt cagttactgg 92640tttacgactg ctcaagctgt ttgtagcaac tatgttgtac
cacaaaatat ctgagccatt 92700acaaaacaga agagtcatta ggcattttat ctccaaccca
aagcatacat gcatgtttta 92760aaatctcaaa ttctcctgac tttaattgtg catattatgt
tcaccaaacc ttttagaacc 92820tgccttgttt tttttgttct ggtctgtagc tgggagtcag
agaaattcaa ctgtgattgg 92880aaaaatggtt actggcaagc tatagagttt ctaagccaga
aggtgaagaa atactacttt 92940tttaacactc ttggcctggg actagactta cagacatgat
caatattgaa aggcaatttg 93000gaggtataca ttttaacatg tcctcagtct ggagttagct
gtgtgtccag tttcctctca 93060gtgtgagtca agcaatagca ttagaaagtt atgccccaag
tctcatcccc tcctattgaa 93120acttggcaca gcacattcag gctgtaagcc accaggtcac
agccccctta aaggattcgg 93180caacagctgt ggttgctatc acatggtgtt gatcatcgtt
gggcccctca ctgtaagaaa 93240gacattgaga ccctggagcg tgtccagagg agggcaacaa
agctgttgag gggtctggag 93300cacaggcctt atgaggaacg gctgaaggaa ctgggattgt
tcagtctaaa gaagaggagg 93360ctcaggggag accttattgc tctctataac tacctgaagg
gaggttgtag tgagctgggg 93420gtcggcctct tctctcgtgt gactagtgat aggactagag
ggaatggctt caagctgcgt 93480cagggaaggt tcaggctgga tgttaggaaa tactacttct
ctgaaagggt ggtcaggcac 93540tggaaagggc tgcccagaga ggtggtggag tcactgaccc
tgaaggtgtt caaagagtgt 93600ttggatgttg tgttgaggga catggtttag tgagaaccat
tggtgaaggg cgaacgaatg 93660gttggactgg atgatcttct gggtcttttc ctaccttagt
gattccatga ttctatgatc 93720attacactgg atttgatact ctgtgagcaa aggcattgaa
gtggtacaaa aaattcaaca 93780ttctgcatta aattgtagaa tctggcaagt ggaaatcgtt
ttctataggc acagccacgc 93840actcagaatg tgtttgcaat ttgcttgcat ttagtcttct
gcaagtaatg actgctttct 93900gtatgcaaat gattgatcca tgtgaaaaaa tctgcttgtg
tatctgtgaa tcaaatgcat 93960tgctttataa tgtgcatttt ggatcattta tttgtggaag
taagtgtaaa aaacagagcc 94020tgcaattgtg cttctgcagt atacaaggcg ttactcaact
ccagctgtac agtcagtcag 94080gccctgagat aatctagact tatactttcc atagttatta
taattttgtc tcttactaaa 94140tctttgattc tgcttgtttg ataaagtaac actcattttc
tatatagtat tacaatcgct 94200tctagaaggc attacatcac tgaattcata ggctttctga
aaaacagatt cagaaatcag 94260attttctaac tgtatttttc catgtatatg tattggagaa
ctagtgaaga acgtgtttaa 94320tatacagaac tacagataaa tccagaaagg agaagcaaca
ctcaaaataa ggatgtggca 94380atcctaaata ggctgtaagc tggcttgaag catgtccctc
caaaaaagcc atctgagaga 94440aaatttctca tttaccatgc atgtgcaagt ttccaaactc
tgcaggtatt ttattttctc 94500cttttgcaaa ttcccttgca gatggcattt tgctttgctt
gctctgaact gcgttgatgt 94560gagcagtgag gtgcttttct catgctgaaa tacaagaata
aagaagattg aagcacaggt 94620ctgtgcagaa catctagtga atgtattcag ggcatgccaa
gcacaagcta ttcaaatatt 94680gctccctgaa aatgcagtca gagtggactt catgttttta
agtggaagtg gtacataact 94740tctgtagtgg agaaatcgtg tgactcaggg ggtgaagggc
ctatcctcag ttaatcccat 94800attcttgttg caatatgggc ctgcatcttc cagcactgtc
agactccagg ttttagcata 94860agatcagtgg aaaaaaatat acacaaatat accccttgct
tctgaagctc tgccctaatt 94920gggatgattg caaataaatg aaaaaaaaaa aaagggaaat
tcaaatactg atgataactc 94980tgcagttcaa caaccaggac acctagtagg tgagttctgg
cttccagtcc ctgctgctag 95040gactattctt gttttaatgt ttaagagaaa acaagtattc
acacatgggt gagtacccta 95100gcaataatga cagagaacta ttctgctcta tagcattctg
atagtatgaa tctcgcctta 95160attccatagt cttctcttag taccacgtcc cccagctcct
gttgtctgaa ttaagcaatc 95220actgtgtgac acctacgtcg gagcttagct ccattacact
caatgaaatc agttgctggt 95280cttctgtgga aaatatacta ttgcgccctg agcagtgctg
agcacagcac ctgtttgcct 95340aatattaatg cagcactcag accacaacca gcctcaagac
actcagcaga aggaatatta 95400tgaaaacagt aggtgctgct cctgaagcat aacagcctcc
agagatggaa gacaagaaga 95460tgtgctttgg tagtgtgtgg tgctcatttc cttgttcatg
aatgatgatg ggaatgactc 95520tggaagacac accagaggcc tctggtgtat accccatgcc
tccagcctgg gcaactcctc 95580cttgctgcct ttttgacttg ttttgtgcaa gccatccatc
cagaggtgca gagtgaaaac 95640aaccatggag ctcaagaaga gcctcatcag gtccatacac
acttcaaacc cagagcaaaa 95700cattggagcc tcgggctcac tgcacagttc tgctgaaaac
tgtgatgaag agctaggggt 95760tagaggaaaa atgtgctgta gttatcagtg cagctccatc
atctgttccg ggagcatcaa 95820ggcttcctgg agagaacatt atcagaagga cacaaattat
tcagtgagag ggagaagtgc 95880gcctctgaac gctctgagtc agatgcttat tttgtgaatt
tttctgtttc cctcttcctg 95940ttatgcttcc tgcagatact tggcacatcc ttgaggcgat
tcagcaatat atgctcatat 96000tcagccacat ctacagagtg cctcctccct gagaggagaa
aaatatttgt tttagggggt 96060aaaaccagaa tagctgtgct tggacctcct gctctgctgt
gggacaagag aagctaggct 96120cctggtaacc tcaggaggca gagggaggca cattataatt
tggctaagac ttgaaaatgc 96180aatttgttgg tatatttggt aaatatactg atggcctagt
cccataaact accttctaga 96240tgtggagtaa gtggtttaaa ggcatagcta agaggttgca
gaaaagaaag gaccacatcc 96300aatttggtag caaccaacat ccagcattca cagactcatg
agaaatacct tttaattaat 96360ttatttatat taaataaaaa aaaaaaatcc tttgatgact
caccctgctt ttcctgttac 96420tctcagttgg gaagaaagta accgctgggt acatactact
gcaatttcag agctgcagac 96480ttgaagagct ttcccaagtg ctgagatatg caggaaaaaa
aaccctgtaa attacagtac 96540caggcattta attttgattg ctaaataaag aagactcgtg
acagtccatg actacgtctt 96600ggagggctgc aattacatat gaaatatagt ctgaattagg
agagttactg gcagaggcaa 96660agtttgcatg ccaattaatt ggtaaaagga gagtacgcca
aacacaggct gtggactgct 96720ctgatgaact gagtatgtaa aaaatagcca tgtgtgtttt
tcagtgaata ccatggtata 96780tgtctggttt gagtcaaata tgtattaaaa tgaaaaaaaa
aaacaacaag aacagtgaaa 96840taaacagtgc tagcatatat tagcttgtat aatcagacct
atatagtttt caaataaatc 96900ttcaaggaga acaaaatgta tagtatgtat gataaggata
agtactataa aacatcatca 96960tgaggagtgc cagtctgaca acaggaaaag gaattcagcg
tgtgaatgaa ggggaaagtg 97020tgactgaaac aattgtcact cagcttacta cagcagaagc
aatcatttat gatcttagat 97080ttttttttat tttttttttt aacttgcttc agagatatct
aagtaatctc aaaaacagga 97140acaaaatacc aacgcaagga aaaattctat tttcgcttca
tataatcttt tctttttttt 97200tctagttgca ttcttaccta aaaacaacaa caacaaaaca
tttaaacaat gtttaaatgt 97260ttactgctgg tttgattaca tcaaaccgag ttgttgctgg
agatgaccag ctatcaaggt 97320gcataatgga ctggcagatg tgcttggtct taccccaggt
tgctgtgcaa acacaataca 97380cattgacata taagctacta tgagttctga agggcagttt
agacattaat tctactccag 97440gccagacacg ctgactatct gagtggttta tagcaaggga
ctggttgact tcaaagtggt 97500tccaagtcaa ccactgccaa gtgcttaaga ctgtgtatgc
acaacagagc tgatcatctc 97560cagtgcaaca aataacatga gagcaaaaag catctgaaat
tctgtaaatg aggctgttct 97620ggccacacct tggctcatta aaagactttg agagatgcca
gaatagcctc tgctaaatgt 97680gatgcagatg gacaagctat ggaatgaatg ggtccagggc
ataaggaaac attaccctca 97740agcactacac aggagctgct gaacaaccac aggaaaggaa
atgtgaaaat gtgaacagat 97800aaatgttgga aagagccgca tttctgctgc ttactatgtc
cttgattatg ccaacattaa 97860ggaagaatgg caaaccccgt gaattggttt aggaacagct
ctacaatgga ctgcctgacg 97920gaggaaaagg gcagcagagt ccttgctgac ctctttctgg
tacaaacaca gatctggaac 97980agagtttaac caattagtct tgcttgcatt catgcctctt
gaatttcaag aggtgccttt 98040gatttcccct ggcctaacac cccatctaaa attacaaaac
catattttgt ctgctgagga 98100ctgtgcacgg atagcccgtt ctggtcaaca tactcaggct
gcttctgcaa caagttttgc 98160actggcattc agtgtagaaa aaatgcaaga cctgtgtagc
ggcagacttc tctctggaga 98220acatgtattg cctcaactat cttacctgtg caaaactgtt
gtggtgactg tgctattgca 98280gaggtagagt gttcaaagaa ggcaaacgta ctgaatgaga
gaacacatca aaaacacctt 98340catgccctct tctaggggag acagcgaaac aaaatgttta
ttgagaaaat cttggacatc 98400agtccaagag atgaaaacac tgtccatatg tgcagggctg
gttgtgttct acaggtccat 98460gctgcataga tgaccacaga ggacaaagac attgaaacca
agcatacaaa gggctgtggg 98520tacccaggaa agttcttcaa ggaagccttg aagggatgtt
tgagtaccca cctgacctgt 98580agctgcaacc ctgatgtaaa catgtgaaaa tgggagcata
agagaagaca ctacacactg 98640caacaaaacc tgtgcccttg gggaggaaaa gtttgacaag
ataaagtaga agctattgaa 98700aaaggaacat taaacaagac aggaggaaag cttcttacta
tctgtagatt tccctactcc 98760cgacatgact actgtcatgt tgacagataa aaaatactca
ttttgagtgt ggaaactgaa 98820agccattcca gttatcatgg tctgcacata cacacatgac
tgaatttcag caacacaaaa 98880cacagtgctt atgataaagg agctcccttt tacctttacc
agtgggtacc accaccactg 98940tgtactgtct gtcttaatgt gcaaaaattt gggatttcta
ttattcattc ccctggcctt 99000aacagaagct ggattttttt ctttagtgct catcaagggc
attattcaat aaagagtaat 99060agctttttac aattgactaa tatttgatat tgtgcattat
gattgtctaa cagaccatga 99120atgttccttc agacagattt ggtagtttat ttacctgtca
tagtaaaata ggaggtacag 99180aagatctatg agaatagcct gtgcatgtac aatgggcctt
gttgccatga cctatgaaga 99240atgaaaatca aaagctgacc accaatcatc ccttgaattc
cactggctgt tcagcattca 99300cttctgaata tctgaatact ctggagtctg ccttcgcaaa
gcagcaaata ctttcagact 99360gttccctaaa tctcttcctc ttacctattc acactgagtt
ctctaattca tcccaacacc 99420tctgctctga attttttcat aagaagcttc agcaaaatgt
gctttctcct ctcaaatgta 99480tgctgcagag cctttggctt acagtggata tagcccaaat
tccagtgaaa aacttcagtc 99540ttgcctaggt gcagaaatag atggagctgt gcttttaaca
agtactaact ataagcttct 99600tcagttctca aactctttca gcagaccaaa acatttttca
gtacagtttt gttctttaaa 99660aaactcataa agctttgttt ctattcttac atggaaagca
atccattaca aaatcctcaa 99720aatagaatga ccatcctgca gctgactctg cttggaactg
cattattttc tctacatcaa 99780gtggttgcca tccatgagaa gcatccctat gtttctctgc
acactgcagt aagagatcac 99840gtatatatca cacttttccc ttcacccatc ttgggagcag
tgctacagta aattgtataa 99900ttacagtgcc ccagagatga gaagaaactg aacagcagga
aaggagacac agtcttaaaa 99960agaagaatgt tttccaggaa ttgatgcact ttcttgcact
ccttggtaat atgggactac 100020tcttgcctca cctttagcag tgggtgctca ttaaatggtg
aatggtggtg ggtcttctgg 100080ttctccaatc atgtcttatt ttctcataat attttgggat
ccttagattc atctgactgt 100140gagaatcact tgatctgatt ttttttttta atctgatttt
gcagctaagt ttatctgaag 100200tgtattatgc ttatcctctt ttttaagggt tttttttttt
tttaaagtgt gtgtattcat 100260tattcgtttg gctctagtta tcgatatggc tcaatcaaat
taatgtttaa attctgaagt 100320agagcatgag acatgctaga cttgaagttg gtacagcttt
ataagataca agaaaagcct 100380gaataattac attctactat taggtttcac ttcacaaaat
aaatttggct ttctccaagt 100440agagtaccag tctaatgttg gcctactcag tgctttcaag
cacaatgaat caaaaggcaa 100500tgacaaaggg tagtaactca aaggatgact cttagaaggc
taacaggggg agtgtccgaa 100560agggtactgt atatatcacc aaggactcag agaatctgtt
caggttcaac tggcaagctg 100620gattattacg agcctctttg atgtttttct gtaagtactt
ctccaaataa aatgtaactt 100680ctaagttgta ttcttgaata tggaaaaaac aaaacaaaac
agaaatatat tatgtaagaa 100740cttagaggaa aaaagggccg ccttctattt tatgatgttg
gcccaccaca tcagaggcag 100800atggtggtgg tatggcagta gaggttgaac cttcccacca
acaccccgtt atgtgttgtt 100860gctgtgtgac agatggcagc agaggggcag tctgacagaa
tggcgtctca catggaagtg 100920tgtatgaagc aaaggtgtgt cactgaattc ctccatatgg
aaaaaaatgg cacccactga 100980cattcatcga tgcttgctga atgtttatgg agacaaaaca
gtggatgtga gcacagcgag 101040gcagtgagtg gtgtgtttca gcagtggcga cagtgacagt
tgttcacctc cactggtaca 101100gaattttgcc agcaggaaat gcagattctt gtacattgtg
ggcaaaaatg catagctagc 101160tgtggtggct atgttgaaaa ataatgttcc gtggctgaga
atctgctcaa ggaaataaag 101220ttattgtaat cattataata atattataca tgtgctttct
atctattgta gtttacatga 101280aaataaatag gaggcattac ttttggtgtg atctgtatac
aggacagata tgtaaaaaat 101340atttctggaa gagaaaattt ttgttttcac agtctcactc
cctgcagaac acaggtgagg 101400tacagtagga taattcacag agccttgtta gcaccaggaa
cctctcaggt tatgtagtag 101460atcacatttg ctacaaacta tggatatgct attattccaa
cttaaaactg ttttagaacg 101520gggagggcac tattcagctt tcttgttctc ggattaaaga
aagagaagga ctgtagattt 101580caataatttc ccctaagtct tgacattaaa ttgcatgtac
aagaccttca cctggctgat 101640ctgatgcagc tttacagtgc attaagtaat ttagccagac
tgtgtattta cggtatatag 101700acgtttgttt gtttttgtca acaacaaaaa aaaggaatca
gcagagatta aatgtcaaaa 101760aatgagaata tagagaagaa gcccactaaa gctatagttt
ggcatctaag caactggcta 101820gatttacaaa gagattcact ctataaatta caggacagca
acctccaatt ttatggccag 101880ttgtacaaag aagcagtttg aaacaagcta agactattgt
ggtttgacta catttgattg 101940aaatatccag agtatggtcc agagtagaca cagaagaaat
gaaatgtgtt tacattgtct 102000caaaaatcat tcagagttct ctggatggct atagggaaac
tcatctagtc cacactgtat 102060tcatcatact gaagcacaga tgaaactatc tatttcctaa
agggcaagta caagatagtg 102120tttttataat gaaccagtac ctttctgaag gaaagtaaac
atgcatttgg gaaacaatgg 102180gtcagtcttt acaatatttc taatgatcac agaattttta
ggctttacat tattgtttca 102240gcatcacaga aacagcaatg aacaagcagc ttctgggcta
caggaagtac tttttactac 102300aagtgccaca cgtcaacacc acacagtaat aatcctgttt
cttttagaca acaccgattt 102360caggatgggc tccatcagtg cagcaaatgc agaattttgt
tttgatgtat tcaatgagct 102420gaaagtccag cacacaaatg agaacatctt gtattccccc
ttgagcatca ttgtagcctt 102480ggccatggtc tatatgggag caagaggcaa cactgagtac
cagatggaga aggtaagtta 102540tgcaagtaaa tacaagctca ttttgatcct ggttaacaga
acaagttatc catgaagatc 102600tttgagactt tctcccctta aggggccagc tgctgtacat
ttgccactgg atttgaactt 102660ggctagcaga aggacattga gccatgaggt ttggatctgg
aactaacttt tcacttattg 102720cttttcacta caaagggtaa caacagtttc tactaaggag
gagatctcct gcttcagttt 102780atattatctc acaaacctga ctccttccag ataaaatgaa
caaattttca tgtataaaag 102840atgaaacact cagaaatcag gagtcacagt tctaagtaca
gtatgggtgt agctggtttc 102900tggatggaaa aataagtgaa ctaattggaa gatcctatca
aaaaatgttc agagcagcac 102960atgcagtaaa aaaacaaaca aacaaacaaa caaaaaaaaa
ccacacaaat ttcaacctcg 103020aatgaaactt ctcagttcag ccattggtta tttcaagccc
agaatttgaa cacaaaatcc 103080agagactctc agtgaacttt gcatacttca tttcttcttc
tgctacttcc atttgcaggc 103140tcttcacttt gacagcattg caggacttgg aggaagcact
cagacaaagg tacagaaacc 103200taaggtacat tatttttctc tcacattcac tttttttttt
tttcctgaaa acttaaaact 103260gttctgactg tgcttccaat aggtccagcc ccttcccaaa
ccctagctaa tgctctcaac 103320acatgatatg caaatgaaaa actaaaattt gttctaaaaa
aaaaaaaaat aatgacaaaa 103380agaaggctca tttcacatgt tgcaccagaa aaagtgatag
gatagttgaa ggacattttg 103440agcaccagga taccttccta cattgataag aacttgcaca
cttgtagggc ttgctggagg 103500accacacatg aaccatgtgt gcttttctcc ttggtcactt
gatacatttg gaaagataac 103560acaagccatg ctcccagggc tgtcctcatc cacttgggtt
ctccaagcac aatgtggggc 103620ttgtaaagga caagaagatt tttccgtttc cttttctttt
tcctttcccc ctttcacttt 103680ttcctttccc cctcactttc tctctccttt tcccacttcc
ctctttcctt tacatttccc 103740actctcctct cctctcctct ccactccact ccactcctct
cctctcctct cctctcctct 103800cctctcctct cctctcctct cctctcctct cctctcctct
cctctcctct cctctcctct 103860cctctcctct cctctcctct cctctcctct cctctcctct
cctctcctct cctctcctct 103920cctctccttt ccattctatt ctttttgcta gagcatttag
atggttatgt agaacaattc 103980acaaaacaca atcagacaaa tcactcacat tttctgtttc
ttatcaccaa gactgagtgt 104040caccaaatgc tatcagttgt acatgcttat atagaacatc
tctcccatgg agcttttaga 104100ctctaatgta ttttgtttgc aaatgtctga acactgtgtg
ttttcctacg tgatctgtac 104160tttataaata gttgtctttc tagtaaaata agctaacatt
tatacccttt ttcctcctct 104220tcaacaaccc agtgtggcaa atccgtgaac atccacctac
tctttaaaga actcctctct 104280gatattactg catcaaaagc caattattca ctccgcattg
ccaacagact ctatgcagaa 104340aagtcacgtc ctatcctacc ggtgagttgt acaacagagt
gattttttgc tagatcctgt 104400ataaacccat aatccaggag tactgcccag agtatctgtt
aatccaactc acctcagcgg 104460tgtggacttc cacagctttt catttgacat tctcaaaata
aaacacacaa atattctaaa 104520tcaaatacat tttatcttta aaaatagaga aaaatgcttc
aaaaataagg attttattat 104580aacaaaacag ttgctaatgg atgctaatgg acctgaagct
gtttttggat tggtatttct 104640tcaagaaaat atttcagcat ttctactacg taatcttatc
tggtaaagta ataaaaatct 104700taaagatctt aacatatcat gcatcgaaat aattttgctg
gcccagtttt aaccatttcg 104760tccaggaaat aagccatgaa aacagtctaa tagcataatt
ataaaaatca tggaacattt 104820taactgcatt ttatttcacc ttcacagtct ttttaaaaac
tgacttggta gctacaactg 104880ttgtctttac agatttacct aaagtgtgtg aagaaactgt
acagagcagg tctggaaaca 104940gtgaacttca aaacagcatc agaccaagcc aggcagctta
ttaactcctg ggtggaaaag 105000cagacagaag gtaagctcag aggagagttt ataatatact
tccttgttac tactttaccc 105060aaacaacttc tggaaagact attccttcca tctccattaa
tggatatttc ctgtggaaac 105120tgatgactct tgcacacttt tttgtgtgcg gtgacagtga
atttaaatat atatgacaaa 105180ggcagggatg ccactgtgtg ctttctgtgt aaggagagca
taactcatgc aagattggtc 105240ccagcttccc tacaatattg gcatcatttt acaagcatat
gctggatgga taagaaatgg 105300gcttccgtgg aagaaaataa tgtggccact aagttggtgt
aagaaaagga atgattaaga 105360gtgtatgtac atttatcagg aaaaaggtgg gaagaaaaca
agaatcaagt attagaagga 105420agcacagtga gaggcagaag atcggtatcc ctgctttgct
tttcacttcc ttctgttcca 105480tgcaagtctt tttccaagga cgtttgagat attcctgggg
atgtgtgtga acattcaagc 105540ctacatgcct ccttacagaa atgcctggtt aagggttagt
tgttctgtat gaaatcactc 105600gtgaacttga attccacatg ccatcattta aagaacagga
agtcaactca agcttgctgg 105660ttgacatcta aaacaaaaca ctcctgcaat gaaaacaaaa
ccccacaaag cagcaccctc 105720caatcccttt gcctcataca tgcaaaccag acagactgtg
tcttagcact cactgctttg 105780cttccttctt acaggacaga tcaaagattt gcttgtatca
agctccactg atcttgatac 105840aacgctggtc ctcgttaatg ccatctactt caaagggatg
tggaagacag catttaatgc 105900agaagacact cgagaaatgc ccttccatgt aacaaaggta
ggggacgtgg tcaccgcttc 105960tgggcaggac agaaagccat caagggtgcg acatacacca
tcctacagtc attggtccat 106020ggttcttctg ggcccctcgc tgacagggca tggggctgag
cccaagacag gctggcaaaa 106080attgtgtctg accaggcatc caaagcacac ctgtagacaa
gagaggaaaa tggagacaca 106140gcttgaggat ccagcccagt tcctctgaag gacttgcaca
tctgcctgct tcaagagaaa 106200ctgccccctt ctcacattgt ctcatgcttc tgttttgcag
gaagaaagca aacctgtgca 106260aatgatgtgt atgaacaata gctttaatgt ggccacactg
cctgcagaga aaatgaagat 106320cctggagctc ccatttgcca gcggagacct gagcatgttg
gtgctgttgc ctgatgaggt 106380ttctgacctg gagcgggtac ggccctggca ggggaagcca
actagttcgg agttcagtgg 106440gagctggctg ctgttagacc tttggctctg ctctcgctcc
ttggctgtgc tgtgctggcc 106500aggcagggga gcacaacagt ggcccaggtg cttccaggcg
ctcaggcaga ggttggcctc 106560taaggagagc cctagcctca atgttattaa acaaagagta
cagcaaagaa tacaaaggta 106620aaggagcgta gggctgctgt aatgttatag aagggcacgt
atgggcaatt cttttcattg 106680agaggcagtt tcatctggcc tcttatataa actcttcagc
aaatgttact agaattgatg 106740aggttcaata atccctaata tttttgacaa tattctcatc
aaatatttta aataagctgt 106800tctcagaata ccaaagtaga tgcagaaata tttgtgtttg
tttggtacta tccactgtat 106860ataaattgtc atggcatttt tttttttgca atctctttca
ccagctgacc aatctgctat 106920gtagtgaaat tgctttattg ttctgtatga gacacgaaaa
tatttgtaca gaaggggatg 106980tgtcaggtgg aaccaaataa aggagcactg aagaggaaat
actagagaaa caaatgttaa 107040aataggaaga tgttgatagg atgcaccttg ggaaactttc
tatttttttg taaaataata 107100gtcttgatta aaatgaacga tggaaagaag ttgcattctc
atcacaggca ttttattctc 107160tccctctctt ttcagattga gaagacaatt aactttgaaa
aactcacaga gtggaccaat 107220cccaatacca tggagaagag gagagtgaaa gtgtacctgc
cccaaatgaa gattgaggaa 107280aaatataacc tcacatctgt cttaatggca ttgggaatga
ctgacctgtt catcccttca 107340gccaatctga ctggcatttc ttcagcagag agcttgaaga
tatcccaggc tgtgcacggg 107400gccttcatgg aactcagtga agatggcatt gagatggcag
gctccacagg ggtgatagaa 107460gacatcaagc atttccctga gttagaacag tttagggctg
accacccatt cctcttcctg 107520atcaaacaca acccaaccaa caccattgtc tactttggca
gatattggtc cccttaaaga 107580gagaaagagc tggcaataac acataccttc ccctcagaaa
caaaatcccc ttaccgtagt 107640attatagcat aatcttatct ctttcataga aaagacatac
ccgcaggaga ggagacagca 107700cgaagcacac ttactccttc ccttcttgta ttaatttcag
aatggcttga tatgagcaaa 107760gactgagcca atgagatggt gagaatgaag acacctatca
gccattaagg tgataagtga 107820ttttcaccca aggaataaat agtaagaatg accctaagtc
cttgggagcc cgttacatag 107880aaagcaataa gctttgctca tcccattccc tggtaacata
ctgctgacaa acccacgtta 107940ccattcctga aacatgggct ttgagatctc cagtctagag
gggatgtttg tggaagagtt 108000tctggtgtgc agattattga tttgtgatta tgtcaatttt
atttttcttt atttggtaat 108060tgggcaatgg tatacatgtt cactatcagt ggagttgtcc
tctcaccata agtcctctca 108120cctagttctg aatttcttgc agaggttttt caaagtcctg
aagagtctcc cttccattcc 108180agagaaggga aatagatcca gttttgcata ggtgcagtta
tgccttttct cagagtgcag 108240attcaaagcc tgaaccatag agatccagat gattcttatg
acccagaact cagtgagatc 108300cactgggcga aagaactgtc taaatttttg tttaaaactt
tggaagacac ttcaaatttg 108360agaacacctt tttggtgaaa aatcctgaaa gtgttgtaaa
atacttcttc taagaaacaa 108420attagaatcc tatttttttt ctgtcttctc ttcctacgta
tagattgtca actgcagatg 108480tggatcctct ggctcaatat tatattctgt tttcattcta
atcacccatt attgatttag 108540atacatacag ttgattttgt tttggttaac ataataaaag
aaaaccacaa acagttttca 108600tgtaaattat attagctttc tgaaccacac actcttaaaa
atatctttac attttaacaa 108660ctgtgagtaa aacgtgattt agcagaaaaa tgtattctta
gaatgaataa agcatgcaga 108720tatgaagttt ttcaggcatt tatgacattt ttaggagtac
ctgttttcaa gaagaacttc 108780accaaagacc tacaaccaga gtttgattct tctctgtatt
tcagatgaca agaagtaccg 108840caatagaata caaagttatt cctatctatt tttctgtgcc
attccaacag gcattaaaga 108900tgacctggca attttttctg gtaaatattt caaggaacaa
ctattctaac agttttaccc 108960ttttatacag aatcacagat tcataggagc tggaagagac
ctcacgggat catctagtcc 109020aaccccatat gatttcatca ttttatatga tgaaataatc
tggaattcat ataacttgaa 109080aggcataaga aaggttaaat agatcaacag ctactaggca
aagcatttcc ctatgcagag 109140tcttgagagg aggaactctg atgttaacat cgcctatttc
cacattagtg ttaccactgc 109200agtgtcaatg ataaaaggtg atctgtagag taaaaacagc
tggtgctaca ggtatgacac 109260ccacattttt tgtagattat caggatactc acaatacaga
cacagctgtt tttcaatggt 109320aaaaccaaac attttaccaa gtatacttta ttttttgcct
ttagaaatgg aagtagtgag 109380aagaacagtt ccaaggtaag agaaatatca gcatctcaag
gtttaccatc agtagtttat 109440tcatctttca catctcttat gtccatagaa tcatagaatc
atagaggttg gaaaagacct 109500taaagatcat caagtccaac catatcctaa ccatactacc
ccaactttaa caaccctctg 109560ctaaatcaga agcagttgat ccactctgct aaatttaaaa
gccagttcac ttaaacaata 109620agaaaactag aggaagatta catttgcaag ctactcttct
acgattaata gactggaaag 109680tgcataaagt acaaagatat gcctcaatga tctgaaaaac
agctgaggtc aaaatggaga 109740aatggggaaa aaaattgaag gtttcttgct tgcaagcaaa
tataaagctt cccctttctc 109800aaaaagaaaa acaagagaca ggaaagagtg gaaattcagc
aatactgaac aaaaattgca 109860acaaaatact gatggccaag ccctggccac cactgaccag
gcagggggca gaacatacaa 109920agggcaggat aaaagtgttc tccatgaagg gggtggcagg
cctggtgggt gggatgatga 109980acaaaataaa tacactttta aaaccttcct gtggctccag
gcatttttgc cccagcctac 110040caaaggctat tagcattttt attttctgga gtattaagac
cctgtttctt tgacagacta 110100ctgtgcagca actgacagaa ggtttagtgg gtaagtcaca
ggggataaaa tgttcagcat 110160agaccaaagc aaaaacataa tgtcatgatg ggtgatcaac
tggctaacag gttgggctca 110220aaggattaca gttactgggg ttacatcagg ctggtaggca
gtcactaatg gggttctgca 110280gggctcaatt ttagggctag ttctcttcag tgttttcatc
aatgacttgg ataaaggact 110340tgaagtcata ctaagcaagt tcatggatga cacaaattgg
gaagtgccat tgactccctt 110400gagggtaaag aggccttaca gagagattct gaccaatcag
agagcttggc aatcatcaac 110460tacataaagt ttaacaagag caggtgtcat attctgcacc
tgggatgggg cagccttggc 110520tgtgtgtaca gactggagga caagaggctg agagcagtcc
tgcccaaagg gacctggggg 110580ttctggctta cagcaagctg tatctgagcc agcagtgtgc
cctggcagct ccaagggcca 110640accgtaccct ggggtgcacc aggcccagca ctgccactgg
gtgagaggag gggctgtccc 110700actgtgctct gtgctatgca gctgcacctc cagcactgca
tacagggttg gctgccacaa 110760cgtaagaaga acacaaaact attagagagc atccaaagaa
gggctatgaa gatggtgaag 110820ggtgtggagg gcaagatgtg tgaggatcag ttgaggtccc
tgggtttgct cagcccagag 110880cagaggagct gaggggaggc ctcatgacgg ctgcagctcc
tcacaagggg agtggaggga 110940cagtgctgag ctctgctctc tgtgacagca tggggctgtg
tcaggggagg gtcaggttag 111000gggttaggaa gagggtgatg aggccctgga acaggctccc
cagggcagtg ggcatggccc 111060caagctgccc gagttcaagg aacatttgga aaatgctctc
agatgtaggg cttggatttt 111120gggtggtgct gtgtggagcc aggacttgga cttgatgatc
cttatgggtc ccttccaact 111180caggatattc tgtgattcta tgacaagatg cactactgtt
ctatgtgtga gatactactg 111240ttctgtgtga gatactagta gccaaggcct tcacagggcc
tttctgaatg tgcctccagt 111300gaatggtcac cggagtaatc ccctctgtca acactgagat
acacatctct gtcaccatct 111360gtgacaggct aaggcagcag tgcaggcaac aatgtcaatc
tcttcagaat ggcacagcac 111420tgctgcagaa aggggtctgg tacgctgtga gcttctgtct
gaaaaacctt gaccaaacac 111480tggtattctt tggactaagg aagcaacata attccataga
acaactgagt gggaaatcac 111540cactgatagc tattgcatca agttctgcaa cagcaactaa
gaaatcactg gcaatcactg 111600tgggcaagac aaacactaaa tggtcaataa gctccctgct
actagaaaaa cagtggaaac 111660ataaatgaga acaaaatctc tagttgtgca aaggtttttg
tggtagaagg agagactttg 111720ttctatgagt tgacctggac ttcatattct tttggaaagg
atcagatgtc aaagagtctg 111780ttagtttagg gacaggccca cagtgaaata cctggtaaag
caaatagcag ctaagtctta 111840gctgacctct ttcatgggat aagcattgtc aaaaaaagga
catgtcacaa caacacatcc 111900ttttgtgttt gtgcagcatt acacagctgc gctggacagc
gtaatccatc agctatccca 111960gaaagcattt cacatacagg aaggtttgct taattttgct
agacttcaca acagatctaa 112020acttgataag tagactacac aagaagtcca atattctgat
gattctgcac tgatgactga 112080ccacgatcca atttttcact tactgtggac tgatttttaa
attctgcaac tcgctttaga 112140ttaaccattc atttgacaaa aagaaaaaat ccaacagcaa
cagttggctg ggatgtatat 112200taactttttc tggaggaatc tgctgcgcct tctctatcac
aaaacaaaaa tattcctctc 112260agcactgagt atatttaacg cagagatatt ttgaaagcca
tataattact aacaacatta 112320gtgctctgaa ttagctatta tgacacaact gtagtatctt
tgtagatcct gagttgtagg 112380ctgtctatga tggcccaaac atatgattca ggcagatggt
acacaaatgc ccagggagct 112440ctcctatagc aagctgtagc atgttgctag acagtttgat
agtaaaagga tttaagacat 112500aatcaaaagg tagaggagac acagtacaac ttgttggata
atcctttgac ttttgagctg 112560tcaagtatac aaccacggac cattgcagtg agtattaaag
cctgtttgaa cagaaaacat 112620gctgattgct agccttaagc aagaaaggga gaaggggcag
cagccacaga aacatcttgc 112680agtgtgagga gtgctctaaa ttgtgtgatt aaagatattc
accatgaaca gacacattca 112740gtcacttgat atgtcttcca ccagcacaga taccaaaatg
gaactcacga cagtggtgag 112800taatttacat attgttgaag caagagaata gctcactccc
tttataatag gtttgatgtg 112860atgggctacc aataagagtt aaggcctaat gatctttact
caaaagtatt gctgctgcat 112920agcaatgtct gcaccagact ggactgggct atagatggta
tcatgtaaca tactagttgt 112980aattaagtgt atcagacaga ctgaggtctt cattattagt
attgctctag catcttcagc 113040tgaacaagac taatgaggac tctattaggc agaaaggtat
ggactattca gaggctgttc 113100actttcacag acaactaaaa gggttaagga gtccacctct
tttctccaga aaacataatt 113160tgttctagac aatttcagag gcattttgta tattgacttt
ggagttctgt tttaaaatca 113220gagcatactc agaggtcaaa gtagtttgtt tgttgcccat
tcttttattt caaaggattt 113280atgagattgc tttatgcttg ctattgtata ttatgactgt
cctgcagacc atgaatgttt 113340cacctgatgt ggcatgaagt tacttgtgaa cgatctgtaa
gaatgttctt tgaatgtgca 113400aaagacacat tttgaacctc acatctggtg ctgtgacctg
tttgaaaaga acaactcaaa 113460tcaacattca aaactagcag tgagttcgaa tacttctctt
gtagcttctg actggagtct 113520gaatatccta atatctgaat ttaaaaagca acagaagtct
cttctctgct caacctcttc 113580tgcgacagta catctttctt cagttctgta tttttttttt
cttttaatac agatgctctg 113640aatattgctt tcaaaattaa tttggattca tacagtatgc
ttgttgatac tttcctactg 113700acaatctgca cagaccatgt tggcacacaa ggtccctgag
ttagactgct ccagcaatgc 113760tagactgctc tgcaaaatgc tttatttttt gcaattcagg
ctgtaagtgg catcaggcac 113820aagaactaga caattacata caagttttca ctgtaggtat
ccctattatt tgcagaggat 113880ttggactaga tggtcttcaa gggtcccttc caaatcaaat
gatgctttga ttctgtgatt 113940ttatgaaaag ttgcagtaag tacagggtgg gcataacaca
gcaaggagtc ctgaatgtac 114000tgcatttttt atgttctcag aatggtgact gctagaggaa
tctggactgt cagtactcat 114060agaggaaaaa aaaaaaaaaa aggaaattga cttaaattcc
ttagagacat tgtgtacaac 114120taaatatcac actttttttt ttttgctttg ttttcactat
ctgtgccaca gtatttggtt 114180ctgtgcttga attatactta gtgttcaagt ttcagtgaat
agcttttatc atttttgttt 114240caatcttatc agtatactcc atccttttct ccaaggtgcc
atatgatatc cttccttctg 114300gaacttttat ttagagactt ctttctttct ttccctcttc
cattctctct ttctttaact 114360ttttcctttc tcctttcttt tttgttttct tttttttccc
ttttatcttt ctttccttct 114420tttttcctta tttctttttc cttctttctt ccttttttcc
ttcaatttct tttttccttc 114480catttctttc ttttttcctt ccatttcttt tcttccttcc
ttccttcctt cctttctttc 114540tttctctctt tctttcttcc tttctctctc tctttctgtc
tttctttctt tccctctttt 114600ttttttttaa ttttaatttt tatttttttt ttgtaaataa
aggacttcaa ccaagtaaaa 114660gtgtgtttct gacactgagt tccatccatc attcagtttg
gcaaacacag aataggcagc 114720atggggtgtg tcatgacatt atacaggata tatttcaagg
agttctgcaa ggctgtacca 114780cgtacagctg agaagctgta ctcttatcat cacaggtgaa
gctgataagg taagcatttc 114840ttttggttat gattcatgtt ctaacccatt ttttaaaatg
atcataagac ttacaagaat 114900actgatggaa ctttgtggtt tgtcatcaag aacagtcaag
aaacaaatga ttaaaggatg 114960acttctttaa aaatctattc ttaccttcac atttctgttc
tgcattactg tactgtttca 115020cagcctgcca catatgaagt caaagtgtta gtacaaagta
aagctatgtt tactaattct 115080gtaacactga gaagctggca ctgtactgag acaccctttc
ttctttttca ttgatgccct 115140ttgtttctga tttagaaatt aaatgcagca ctgaatttgt
ttaaattcaa gacttaagct 115200gagttgcatg gtctacctaa catactttct gaatgaagtt
actgaatgca gcatggtcag 115260gtatcaacaa catactgcaa attaatttct gtgtattcta
aaacaagcaa acgaacaaac 115320aaaaaacaca cacacacatg cacaaagcat ttgcttcaac
agtatgtttt ttcaacaaga 115380tcatacatgg agcttaaagc ttaaaatata atactctgtg
ggagtagtaa ataatccaga 115440agtttgccct ctatcacctg cacatgtgat tcaattaaga
gagagatgga acacatgaat 115500gtgttgattc cacacaatga aacatttggc agaatatctt
ggatttcccc tgtacttggg 115560aaattctacc ctaggaagat tctctctgct tgtgacaaaa
tgggaagata taaggacctt 115620aatactgcac tttacagcac tgttgtctat tctatgttgt
cttctttact aaagagtttt 115680ttttttcctt tactgttaga taaaatgata tgtgttgaaa
ctacagggaa aatttcatta 115740gaatgtcaga aaaaaaagac agaaaaaatg tttaaatact
gacgatgtga agtatctgca 115800aatgaaacaa gcctaaacaa tcactgcctt attaaaaggt
ggattttatg aaaaaggtgc 115860caataaaatt aaagaacaat tttgaaaagt gaggtataat
taagtcaacc aagaatggaa 115920catgtaatat ttaacagaca tttgtcataa agcagatgag
tttggtaaat cattatctct 115980ttctatcact gtgcttccat ttccctaatc tatttttaag
aaggtaatga tgaggtttga 116040gacctctgat aaagtggttg gtataagaat ccagcttcca
tttacatgaa ggtggagtaa 116100atccagaaaa aaacttgcgg tgtttttcca gacctaccca
ctttatattg tcaataactg 116160tagtttggat cacagagggc tgatctgtta actggtctta
aaagtgatgt taaaaactat 116220agtgaaaaac ctggtctgga gtctcaggtg aatgaagact
gagaacaaac ctatgtgtgt 116280tttctttcct gcacaagatg ggaaacgatt gtcaatgagc
ttctttcaag gcaagtcttt 116340gcaatatttt caacacagta cacatgtaca gaggataact
caagtttcaa ataaaacagt 116400tgccagccta cacataactg gtacctatac aagattttga
ttgctcacaa atccaagcac 116460acacctgcct tttaaatcca cactactgaa ttctacttac
tgaaaataag ctgtgcactg 116520tgtacagagg ttcaagtgca ctgacttcct tggaatacaa
ctaatacatt ttaatctttt 116580ctttagacaa cgatttcaga atggattcca tcagtgtaac
aaatgcaaaa ttttgttttg 116640atgttttcaa tgagatgaaa gtccatcatg tcaatgagaa
catcttgtat tgccctctga 116700gcatccttac agccctggcc atggtctatc tgggggcaag
aggtaacact gaatctcaga 116760tgaagaaggt aagttgctta cattggtgta aagtggacag
tggactctac ttctgcttgt 116820cattccttct aagtaataac atattatcta ctcatgaggc
tctcacatat tttaattcac 116880cagatggatc atgaatcagg gaattgtatt atttttttct
aaattctgac atcttccaca 116940taatgtgatc attttttttc catatttttt atttttgtat
taaaaagata aaaccctgga 117000ggaaaggaag agggagaaca ttattcgcag tgcataatac
acaactaagt taacatccag 117060atgctcactg aaaaaaatat aatctaagca aatagtgcta
tttccaattt ctcagaaggt 117120gacatgaagt atgaaccagc tgcaagctta cttgcagcct
tttagttcat ctaatctagc 117180atttgttgtg ggtttttttt ttgtttctgt ttttgagcca
acagctctac cccgaacatc 117240acgtgtaaat tttaaatgca taccattttt ggtcacgctt
gtgttttttt ctcactggca 117300ttttctcttg caggttcttc attttgatag cattacagga
gctggaagca ccactgactc 117360tcaggtaaag atgtaacttc tctccttttg ttcctatttt
ctcctcagga caaaactaga 117420actactctgc ctctgctcca agcagtttca gactgtcaaa
agtggtggca atgctctcaa 117480accaaacaga tctgtggagg gaggaaaaga gtgtgtaact
cactcttgtt taaagccagg 117540gaaactgact tggagatagg tttatttgtc tgtttaatgc
accatcatca gactaggtct 117600gtgggtgaat tccaccatgg cctgactgtt agtgatgggg
acagtccttt ggggtctgat 117660tttctagata aggagaaact aatgtgacat atcatcttgt
tttcctgtca tcacctcagt 117720gtggctcttc tgaatacgtc cacaatttgt tcaaggagtt
actctcagaa atcaccaggc 117780caaatgctac atactcactc gagattgctg acaaactcta
tgttgacaaa acattctcag 117840ttcttccggt gagttgaagt gtgacttaac ctcagtgaga
ttgcccactg ggctcacctg 117900ggactcggct ctactgtgag ccacaatggg aattggtttg
agccacagga tgagttcaaa 117960cctttctgtg gcttttagga ggaggctagg ctcacacaag
gtataagggc tctggagata 118020ttcaagaccc atttggacac tttcctgtgc aatctataat
gaacccctgc agggggttca 118080aattgatgat ctccagagat cccttccagc ccctgcgatt
ttgtgactct gtaatatatg 118140cccatgcagc aactgctaca gggagcaatc aaaattgctg
ctcattcact aaaaaatgtc 118200tcttaatgaa aaaggtgatt tgtaagggag gaaaatgact
tgaaagcgtg acgactgaaa 118260ttgacaaaaa tattttgttc atcttttcta aaactagaca
taaaataact cacttaaaga 118320aagttttggt tttgaaataa aaaacaggaa tgtaagaata
cacagttcaa aagaaaaggt 118380aggcacgaag atgaggaaat gagtattgtc tgtccttaat
aatgtttgca gaacagaagg 118440ttttatggta aaatgaagaa aatatttcaa aattttaact
tagaatccaa tctgaagaca 118500aaagtgacaa atctaaatat gtgaagtagc cttgtccagc
tttaagattc agttacagca 118560agagagctgt ttgacttgtt caagtgtagg gatagaagtt
tcttttaacc atcactttcc 118620atttcattaa ttttgcattt catattcttc tattttaaag
ttctcaacag tcaaacacaa 118680ttcttctgct tataggaata cttaagttgt gcaaggaagt
tctatacagg aggagtggaa 118740gaagttaact tcaaaacagc tgcagaagaa gcaaggcagc
tcataaactc ctgggtggaa 118800aaagagacaa atggtaagaa gtaaaaaaat agctgatatt
ttctcctacc tactgtaatc 118860tacgctcttg tcttcttctc ctcaaaatgt gaagaaaggc
atatcaagga acagcacttg 118920attattgcta tgaaagcaaa ctcccataaa actcaccatg
cccttcattg caggcattca 118980tgcaaaccag acaggctgtg tcttaacact cactgctttg
cttccttttc acaggacaga 119040tcaaagattt gcttgtatca agctccattg attttggtac
aacaatggtc tttattaaca 119100ccatttactt caaagggata tggaaaattg catttaatac
agaagacact cgggaaatgc 119160ccttcagcat gacaaaggta gggacatggg cactactact
ggaaaaattc aagataaagt 119220gatccctact cacattgtct catgcttctg ttttgcagga
agaaagcaaa cctgtgcaaa 119280tgatgtgtat gaacaatagc tttaatgtgg ccacactgcc
tgcagagaaa atgaagatcc 119340tggagctccc atatgccagc ggagatctga gcatgttggt
gctgttgcct gatgaggttt 119400ctggcctgga gcgggtacgg ccctggcagg ggaagccaac
tagttcggag ttcagtggga 119460acttctgact gctttcagac ctttggctgt cctctcaccc
cctggctgtg ctgtgctggc 119520caggcagggg agcacaacag tggcccaggt gcttctaggt
gctcaggcag aggttggcct 119580ctaaggagag ccctagcctc aatgttatta aacaaagagt
gtagctaaca aaacaaaggt 119640aaaggagcct agggctgctg tagtcctgca gcaggggatg
ttggtatatg caagttatct 119700ccatcaagta ctagagacag atatgctagc aggatttctt
tttttacttt gaagaaattt 119760caattcccag agatcaagta gagttcaaac actgttacca
agtcataggg accaattctg 119820ttgatgaccg ttaatagatt tttttcatga gtcacccctc
caaataatta aatataattt 119880tttttttgta aatatgaggg atattttaaa tgatcatttc
tcattgaatg tagaaaaaaa 119940taggaaaaat ataacaagaa aacaaacagc atttctgaga
ggttagctgc aaacatctgc 120000aaatgagcaa aaatttgatt tgacataatc aaaaactgat
ttttcagaaa agcatttgat 120060ctgttggaag aattttcaga tgacaaagtt ttggagagct
tcatcaagac agatgatatg 120120taggctatag tcaggaagaa gcacaaggga taaacaaata
gatttaagct taagcgtcac 120180ttctgttttg cacacaaata aatgaaataa atagcaacaa
gtggtattaa tacagttggt 120240atggccacca tactcctgct ttatgcattt cattgtctct
tctctttgca gattgagaag 120300acaattaact ttgacaaact cagagagtgg actagtacca
atgcaatggc aaagaagagc 120360atgaaagtgt acctgccccg catgaagatc gaggaaaaat
ataacctcac atctatatta 120420atggccttgg gaatgactga cctgttcagc cgttcagcca
atctgactgg catctcttca 120480gtagataacc tgatgatatc tgatgctgtc catggggtgt
tcatggaagt caatgaagag 120540ggcactgagg cgacaggttc aacaggggca attggaaaca
tcaagcattc ccttgagtta 120600gaagagttta gggctgacca tccattcctc ttcttcatca
gatacaaccc aaccaatgct 120660attctattct ttggtagata ttggtcgccc taaagagaga
aagagctgga aataatgctt 120720accttcccct cagaaatcaa acctctttac tgtagtattg
tagcataatc tcaatgcaat 120780attttatcca agtggaaagc cttcaatatc tagggagaca
ttcttgaaga agcatgtgaa 120840atttcagatc tttatatgca ggaatttatt ctcagcttag
attcaggatt catatccaag 120900gtgtacatat tcccaatgtg cttgaataac ttgggaaaca
gggccagtgc tttggggttt 120960tttttgtttg tttgtttttt gtttttttgg tttggtttgt
ttttttctgg ttggttgttt 121020tttttttttg tgtgtgtgtg tgagattctg ccattgttat
tgagaatctg gtttctctat 121080aggagttctc tgaaataaac acagctttca ggaaaatcct
ggtcctttcc attgaattag 121140ctgggcagtc atcctagaac tgatgcctgg acaacttgca
gatgaaattt ttaacttcag 121200cagaccattt gtcttccagt aatccatttg gacttattcg
tgctgcgtaa cattttttct 121260gagggagcat acagaaagtc taccatttct tcttaaatca
tctccaaaca aaacatcttc 121320ctgattgata ttatttccca ttttcatccc agtgacatgt
cactgatttt gtgaatgtta 121380attaatggtc tttctattta ttctaataaa agcttcgcaa
acaaaacatg tcattaccta 121440ttctgggtta ctgtactaca caacctgaaa aatacgatat
agcgggtaat aattattgac 121500agaggtgact aagctggtat gtggatccta ttttcaaaat
cagaatgtac ccatatatga 121560ggtcactaaa tattttaaga ttaaaaaaaa aaaaaacaac
tgggtttaat caaggtaaac 121620cctatagctc ctactcttca attgagcttc tcccaataca
gcataccaaa taacaaaatt 121680ttttgaattt actgaatttc cagagaactt ttacagaaat
cctctaaggg tcctcagtaa 121740atacatgaag gtgatgtgta caagatagaa ttttaaaata
tgagaaaggt attaaaaggt 121800agactgcttc agctttctca tgctgacaag aatcacatga
agaaatcttt ctattgcctc 121860atgtgatatt cctctcgaga tgttgtatgc tatttcacgg
tctttagagg aaagggtctt 121920taggttatat attatccaat tataatggtt actagtgtta
atgacagttt tctgctagga 121980tacacatgca gaattgcaaa ttacagtatt ggtactgaaa
atgtagcgat acttcagtaa 122040ttcagagctg cctcaaacac atgccatgtc agcattaact
ataacttgaa atgatgacat 122100tagcagcagt gaaacacgtt tcatacccac taaaaatggg
agaaatgcga ttactggtct 122160tcccaagagg gtcagaagga ttaggtacag tttgcacaag
gattcaagta aaaaaaagta 122220tttcaagagc tatgaaattt caagttttac tgtgtacatc
atgttatttc cctcttacag 122280ctgaaatcag tcaatacagt tttgctacaa ctaaaaccaa
ccaaccaaac aaacggcaaa 122340ggaaaaaagg caggactggt aaaatattgt cctgagcagc
agagtgtgta aggttactaa 122400gcagttataa tactgtgttg gaactgaaat atgcagctat
gtcactctgc actttctagg 122460tacaataaca gtaaaagtac ccacatttac tatggaggta
tttaatatat agcgtaaact 122520aaaaaaacag ctattcaatt gtttgatctt tttaaagcaa
aagatgaaga aaatcaaggc 122580agaaatgata aatgaatttc aaaaactata caaaagaggg
tattcaaggc acagagtcca 122640cctaatgtct taactgtgaa atggaagcat ttgacttgtt
ttaaaaaggc cattaggtgt 122700cactaagggt aaaaagttac gtttatcagc tttcagaaag
aggatggcat tccaaaggtg 122760cctctgagct ctgaagccca gcaagggata agagaaatga
atcccaagtc cagctattgt 122820ccaaaagtcc tttttgttcc ctgataccat gactgaagtt
gtcatgtcaa gacattgcct 122880tctgtctgtt cactacacct catgttctct cagtgctgtg
ttcttagaga ggcagtactg 122940ctagtggtcc gcggaatgaa aacagccagg tgtaatcaca
ctcttttgaa tgcctcatga 123000gaagtgctct ctggactaag tgtaactttc cttctcacat
catttggaga agggaccatc 123060acagaatcat ttggagacca cctgcatcta ttctgcactt
ctctccacat ttgcctatcg 123120tcttctaagc aaaccgaatc tatggctgaa gttacaagac
tctgcatgtg gtgtcacagt 123180caccagaggc agaggactca agagaatatc ctggtccaga
gctactctgg gatctcaaaa 123240gtcacttggg aaagcacagc atgttgaact agactttggt
gtatttttcc aatctttatc 123300agtctacaaa atatcaactg gacatggagc agtggttcac
tttggtgttt cctgtggctc 123360atgatctaag attcttcagt ctggaaaata aactgcagag
attactgttg aggagcagca 123420agtgtcagtc tgctcagcaa gggaaggaga ctgagggcaa
gggaaggaga ccgtgccagt 123480aaggctagaa gggcccgata agggatcaag tgctaaattt
tccaatatta tagccatagt 123540ttagtgattt ccagagaaat atgtagtgat gtggtaggcc
aaagatcaaa ccagaaggcc 123600ccagaatggc aacaggtgat gatccctgtg gcacattctc
tatcttgaag taaaacagca 123660tggatccata taaatacatt cttgctcaac agcagaaata
acaaacagta ttgcttactt 123720ctacgaatat cctaacaaaa catgtagatc acaatgccac
tgaacctttg tatggatgga 123780atctgtgcaa tctgccatga ctaaagctct gtccaaaact
gcacaactta gggtgcccag 123840cttctgaagg gatgtgaaat tatctgtgct atctcctttt
cccttcttgt gttagctcca 123900gtaaactcta ttttaagaaa taccttacag tttctgattg
tcttctttac tggtatccaa 123960agggactcct atgcattaca gggtcctcca gcacagtgag
gttcttggcc tggtgcaggc 124020atgcaaagta gcttaggcac gggtcacaat caagatactc
agtttaatgc ttctcccaag 124080tgatgggatg ctaaaatctt acatgatttt aaaaggaaag
tgttcaaact gtggaagaga 124140aatccactga caaataagaa agatacagaa aataaagtta
gctatagaag acatggaaca 124200ggaaataatg ttagaactct gagggcaaga gtaagcctta
acagtaatga caagcctcac 124260tggaggagct cttccacata cgttgttctc atgggcccag
gagtctgtac tggaaattgg 124320cacacagttt gggtaccggg ggcgatcttt gtgagatgaa
gccctgaact gccctgggtc 124380agctgcaggt gtctctgtaa tggatgaaaa caactcactg
tgcaccagat tttcagctaa 124440taagaaaagc acatggcatc tctgctcaaa cagaatcata
gaattgctca ggttggaaaa 124500gaccttaaag gtcatcgagt ccaactgcaa cctaaccaac
taccctaact ctaacaaccc 124560tcctctaaat catgtccctg agcaccacat ccaaacagtt
tttaaacaca tccagggatg 124620gtgaatcaat cacatccctg gggagcctat tccagtgctt
aacaacactt tctgtaaaga 124680agtttttcct gatatccaac ataaacttac cctggcacaa
cttaaggcca aatttaatta 124740gaaaatgtag cagcactgca atgtagcaaa tgtaattacg
aaaaggtggt agctgctagg 124800gacagaggac atgcaaatag acccaaaaga taaagactag
aaacagaaaa aggggacatg 124860tgagaggtat gtttggagaa acataacaga ggagatattt
gaaaggagat cttgggagca 124920caggcaaaga cacaatcctg ggaggaggtg ctccatgcta
gaggatgtac ctctaaggca 124980ccgcagccat gggcaaccaa cacaggtcag cgtcatcctg
gtgagactgt atcccacaag 125040cagctaacac tggagtaggg acagccccga agaactgcag
cccaggcagc acactagagc 125100agagaaatct agttagcagc aaccactggc agacagaaat
gattatatag attacatact 125160gaccctagcc tcttacactg cctactgcat cactgaaagg
actgggaaga agagagtgca 125220ataacgtagc tgaaactagg aggaaggcaa ggagaactga
agctgactag ggaaaagggg 125280gattaaaggt ttaagtgtct attccatagt ttgctggttt
gttttttgtc aattcctgaa 125340tcagtaattt ttatgttaat tagcaaaaaa ttacaaacac
tccccaagtc aggactgtta 125400cctacaacag aagctcagat cagctgagcc ttagtctttt
ggtccctccc tagggaatgc 125460tgtatgtgtc tctctctcca ggcctgctca aaattgacct
cagacccaaa cttttgctga 125520atctccagta ccacctcttt tgctcctaac tagataacaa
agccctgagc gctttgcttt 125580tagcaaagcc tttaagtgcc attaccaact gcacctggag
cctttaccta cccctatgga 125640cccaggctct atatttaagc tctgccctga accttcactt
ctttcctgtc ctaagttaga 125700tgtactagta tggtgtgtac tatgtctcca gttcaaacac
agctgtgccc atacctggcc 125760aaggactcct agtatgacct gggctgtgcc ttgctgctaa
ggacctgctg ggtgattgct 125820ggacctgatc ctaatcctga attaagaaat gatttcttgg
cttgactgga tgtgccctgt 125880ggtatgatac tgccttatga tttggactct tgtttgcagc
tgtgcaaatc cctaaggagc 125940ccagtctctg gccacctgga atcttgtcac taccaaactt
cctgagggac tggtcttgct 126000ctgggttctg atctctggac agtactcacc ctttactcag
cccaggctcc cagttaagcc 126060cctttccacc ctgccaggct ctccgctcca tccctagcag
gggctctcat gacagtgtga 126120ccccccctta ctcaggtcag ggccacttgt gccacgttcc
tttcctgtct tctgtccctg 126180ccttggctct aaagcagtgt gctaccatcc acaaccactg
catctctcta aagtaagcct 126240ctcctgagcc caagtctctg taacgaggaa ggatgcactt
tgctcagaag gatgcgaggc 126300tgcttctgag ctctgagggc actgacctcc catgaggtac
accccatacc caggaccaca 126360attcagcctg ctggaaccat caactcctgc tggagtaagg
ccatagcaag accagcatcc 126420acctccctgc agccctgccc tgcccagata ttgggcctgc
tgatctcagg atgcagactt 126480gcttctcagc ttgacctaag cattgccctg tctttatgga
cccacctggt tagcaagttc 126540agtgcagaag gaggctgttg gcatctagct aattttccac
ccacattact gtctgctgac 126600tcattctacg tctctcccat cttgttacaa taataatttg
ggagatcata ttgaaggtct 126660taataaagtc aaggcatgtg atattctctg ctttgccttt
gtttctagaa taagccactt 126720catcatagaa gatgaaaatg ctgatcagca gagatctgtg
cttgataaat ccatgctggc 126780ttttcctatc accttatatt ccttcatatg ccttgagaca
cccaaggagg ccttggatca 126840gagctgtctg tagcagtcct aactggtata caattagttg
tacaacaggt agtgatccgc 126900ataatagttg gcgtgagaaa gtgggcctgt gctgtgtcaa
gcatagagtt tgggttccag 126960tcctgttctg catggcacat atgcctgagc agctgggtaa
tctctgcatt ccaattggaa 127020ggcaggggcc tgtaggcagt tcccacttgg catgggtgat
tgtaccacct gtgtcctcat 127080ctgtgaagca tcatgttttc attcaaatat ccttttgttt
gacagtagaa atgaacagaa 127140ttgttttttt ttcctaagca aattctgcaa gagctctgaa
gaacaaggtg tcagtgaact 127200tctagctcca tagataggac ttgcatcaca tgtcatgcct
tgattggagg tctatccgat 127260actgaacaac ttgtggttcc ctgagggaat gtaagattac
tgatactact ctctctttat 127320gttagctaca ataaatggta ggttaagcaa tagatacaga
gtttgagtgc ctttcttaca 127380agcatcatag tgaacaaatc cactggtgat ctaccttttc
aataactaca gagaattgta 127440atctcttgga ttctcctcct tccccgttct gaaaatgtgt
tctttttttc caaatcagaa 127500accttcctca accaccctga ctattctttg gacattgttt
tgttcttgct cctaaatagg 127560ctttataatt tttgtaagtg aaaggctttg catgcaggtg
aggctacaac tcattcagta 127620acaatgagga agactgtcag attttgggga aaattctccc
acccaacctt ttgctagcca 127680gtaagatgta atcactgaat gtcatgccac aaagaccata
ccaacatcag accacatatc 127740tacaggaagc tttaaggaat cattgactgt acagtgaagg
gtaaatcaaa ttaaaatgaa 127800tgtgaggtct gatacgagat atcctcatgg gaatcaagag
caaagacaaa tagtttttca 127860cagtcttgtc atgatctgtc acagaccaag gcagcacagc
aggcaacaat gttggtctct 127920tcagaatggc acagcaccgc tgcagaaaaa tgccaggtgg
actatgaact cacatccaaa 127980ggagcttgac ctgatacctg attttcttca aacaggggaa
acaacacaat cccacaaaac 128040agctcagaga gaaaccatca ctgatggcta cagcaccaag
gtatgcaatg gcaatccatt 128100cgacattcat ctgtgacctg agcaaaatga tttatctctc
catgaatggt tgcttctttc 128160cctcatgaaa aggcaatttc cacactcaca atatgcaaca
aagacaaaca gagaacaatt 128220aatgtgctcc ttcctaatgt taaaattgta gtggcaaaga
ggagaacaaa atctcaagtt 128280ctgagtaggt tttagtgatt ggataagagg ctttgacctg
tgagctcacc tggacttcat 128340atccttttgg ataaaaagtg cttttataac tttcaggtct
ccgagtcttt attcatgaga 128400ctgttggttt agggacagac ccacaatgaa atgcctggca
taggaaaggg cagcagagcc 128460ttagctgacc ttttcttggg acaagcattg tcaaacaatg
tgtgacaaaa ctatttgtac 128520tgctttgcac agctgtgctg ggcagggcaa tccattgcca
cctatcccag gtaaccttcc 128580aactgcaaga agattgttgc ttactctctc tagaccccca
agtcaaacca actatgcagg 128640tatgctgaca acactatgat gacagcctgt tctgatcaag
atctcatttg ttcatggaca 128700atttttgttg cttgcagctg gtcttccatt gggaaagagt
gtagtatatc cttctcatct 128760gacagaaaag cagaaattct catgctccac acttaatcta
cattgtttta aaccaccggc 128820tacttcttgg agaggaaaaa tggcttttat aagactcaca
aaacaaagct ctgcaagtca 128880aatgcataca aaactgttct gtaggtctgg aatcaggaca
ctatgtggaa gtcaaataga 128940gcagctttaa aaagcctttg ggatcattct catcttatat
ttgcagcacg atactatgac 129000agtgataact gacataactg catcaatttc cttgatattt
tatttgtctt aaagtacaag 129060acatagagat ggacgtaaag atggacatat gactcaggtc
tggacaggtc cgtggtccat 129120gtatgataaa agagatgaag ggaaggagaa ttgagactgt
ctaagaaggg cttcagggac 129180gttctgaagg cagatttgac tgaatcagat gtactgtcca
agtctcatat gtagcaatgg 129240aaggctgata ttggagaaat ataaagaaat ggctgtgaac
tcaaagtgac cctgaacaga 129300aaagggatat ggagttaaaa taatgtcaca gaactgaggt
ttatatgata taccatgggc 129360tgcagagggt cagagtgctc caccatgggc ctctcttggg
ctgcagggaa cttctgttct 129420acacctggaa cacctcctgc cctcctccgc actgacctca
gtgtcatcag ggctgtttct 129480ctcacatttt ctcactcacc tctcccaact accattgtac
agcagttgtt cttacatatt 129540gctcctcctg aggtacatct agcatcgatc actggctcag
ctctggccag tggcagctcc 129600cttttgagga cacgggacag ctgctgggct ctgttcacag
aggccactcc ggcagacctc 129660cactaccaca acttgtagtg taaatccact acaactttct
gagctacaga aatgaaatgg 129720agaccctctc tgctatggga tacaaaagag gaaacgtggc
gtttagctct ggctcactgg 129780tacacccaac cacagggtga gaagcagcct gttgttattc
actactctta ggacagatta 129840tggtgaattg ttaataaaag catttcttca taacatccaa
aggaggaaat acactaaatt 129900atatttttta ttaattaatt acacatgctt aattatatat
ggcatggttg ctttggaaga 129960atcttgtcct tactgaccag atctgctgtt tgctgagaca
aaatggctga caattttggc 130020catggtggat accttccccc ttttctgtag cattaggaca
gaagttattc tggagcctgt 130080ctgacaagtt agacttgata cctttaagta tttggaagtg
tgcttttcat gctggatgtc 130140atctccagaa cctccctgtc tggtaagcag ttccctgcct
tagtaagagc cgaaacggtc 130200tctcttttcc ttgttatctc accaggatat tacaatgtga
caggactatc tgaactatgc 130260caacctgcaa attccaaata tatatatata tataagatat
ctatacacaa attattagtg 130320tttgattgac accagatgac agagaagtgc atctgagaaa
acctattccc aatctccttt 130380ctctttctgc agactgacat gcatttcata ggtagagata
acatttactg ggaagcacat 130440ctatcatcac aaaaagcagg caagattttc agactttctt
agtggctgaa atagaagcaa 130500aagacgtgat taaaaacaaa atgaaacaaa aaaaatcagt
tgatacctgt ggtgtagaca 130560tccagcaaaa aaatattatt tgcactacca tcttgtctta
agtcctcaga cttggcaagg 130620agaatgtaga tttccacagt atatatgttt tcacaaaagg
aaggagagaa acaaaagaaa 130680atggcactga ctaaacttca gctagtggta taggaaagta
attctgctta acagagattg 130740cagtgatctc tatgtatgtc ctgaagaatt atgttgtact
tttttccccc atttttaaat 130800caaacagtgc tttacagagg tcagaatggt ttctttactg
tttgtcaatt ctattatttc 130860aatacagaac aatagcttct ataactgaaa tatatttgct
attgtatatt atgattgtcc 130920ctcgaaccat gaacactcct ccagctgaat ttcacaattc
ctctgtcatc tgccaggcca 130980ttaagttatt catggaagat ctttgaggaa cactgcaagt
tcatatcata aacacatttg 131040aaattgagta ttgttttgca ttgtatggag ctatgttttg
ctgtatcctc agaataaaag 131100tttgttataa agcattcaca cccataaaaa gatagattta
aatattccaa ctataggaaa 131160gaaagtgtgt ctgctcttca ctctagtctc agttggctcc
ttcacatgca cgcttcttta 131220tttctcctat tttgtcaaga aaataatagg tcaagtcttg
ttctcattta tgtcctgtct 131280agcgtggctc agatgcacat tgtacataca agaaggatca
aatgaaacag acttctggtc 131340tgttactaca accatagtaa taagcacact aactaataat
tgctaattat gttttccatc 131400tccaaggttc ccacattttt ctgttttctt aaagatccca
ttatctggtt gtaactgaag 131460ctcaatggaa catgagcaat atttcccagt cttctctccc
atccaacagt cctgatggat 131520tagcagaaca ggcagaaaac acattgttac ccagaattaa
aaactaatat ttgctctcca 131580ttcaatccaa aatggaccta ttgaaactaa aatctaaccc
aatcccatta aatgatttct 131640atggtgtcaa aggtcaaact tctgaaggga acctgtgggt
gggtcacaat tcagactata 131700tattccccag ggctcagcca gtgtctgtac atacagctag
aaagctgtat tgcctttagc 131760agtcaagctc gaaaggtaag caactctctg gaattacctt
ctctctatat tagctcttac 131820ttgcacctaa actttaaaaa attaacaatt attgtgctat
gtgttgtatc tttaagggtg 131880aagtacctgc gtgatacccc ctataaaaac ttctcacctg
tgtatgcatt ctgcactatt 131940ttattatgtg taaaagcttt gtgtttgttt tcaggaggct
tattctttgt gcttaaaata 132000tgtttttaat ttcagaacat cttatcctgt cgttcactat
ctgatatgct ttgcagtttg 132060cttgattaac ttctagccct acagagtgca cagagagcaa
aatcatggtg ttcagtgaat 132120tctggggagt tattttaatg tgaaaattct ctagaagttt
aattcctgca aagtgcagct 132180gctgatcact acacaagata aaaatgtggg gggtgcataa
acgtatattc ttacaataat 132240agatacatgt gaacttatat acagaaaaga aaatgagaaa
aatgtgtgtg tgtatactca 132300cacacgtggt cagtaaaaac ttttgagggg tttaatacag
aaaatccaat cctgaggccc 132360cagcactcag tacgcatata aagggctggg ctctgaagga
cttctgactt tcacagatta 132420tataaatctc aggaaagcaa ctagattcat gctggctcca
aaagctgtgc tttatataag 132480cacactggct atacaatagt tgtacagttc agctctttat
aatagaaaca gacagaacaa 132540gtataaatct tctattggtc tatgtcatga acaagaattc
attcagtggc tctgttttat 132600agtaaacatt gctattttat catgtctgca tttctcttct
gtctgaatgt caccactaaa 132660atttaactcc acagaaagtt tatactacag tacacatgca
tatctttgag caaagcaaac 132720catacctgaa agtgcaatag agcagaatat gaattacatg
cgtgtctttc tcctagacta 132780catgacccca tataaattac attccttatc tattctgcca
tcaccaaaac aaaggtaaaa 132840atacttttga agatctactc atagcaagta gtgtgcaaca
aacagatatt tctctacatt 132900tatttttagg gaataaaaat aagaaataaa atagtcagca
agcctctgct ttctcatata 132960tctgtccaaa cctaaagttt actgaaattt gctctttgaa
tttccagttt tgcaagccta 133020tcagattgtg ttttaatcag aggtactgaa aagtatcaat
gaattctagc tttcactgaa 133080caaaaatatg tagaggcaac tggcttctgg gacagtttgc
tacccaaaag acaactgaat 133140gcaaatacat aaatagattt atgaatatgg ttttgaacat
gcacatgaga ggtggatata 133200gcaacagaca cattaccaca gaattacttt aaaactactt
gttaacattt aattgcctaa 133260aaactgctcg taatttactg ttgtagccta ccatagagta
ccctgcatgg tactatgtac 133320agcattccat ccttacattt tcactgttct gctgtttgct
ctagacaact cagagttcac 133380catgggctcc atcggtgcag caagcatgga attttgtttt
gatgtattca aggagctcaa 133440agtccaccat gccaatgaga acatcttcta ctgccccatt
gccatcatgt cagctctagc 133500catggtatac ctgggtgcaa aagacagcac caggacacaa
ataaataagg tgagcctaca 133560gttaaagatt aaaacctttg ccctgctcaa tggagccaca
gcacttaatt gtatgataat 133620gtcccttgga aactgcatag ctcagaggct gaaaatctga
aaccagagtt atctaaaagt 133680gtggccacct ccaactccca gagtgttacc caaatgcact
agctagaaat cttgaaactg 133740gattgcataa cttctttttg tcataaccat tatttcagct
actattattt tcaattacag 133800gttgttcact ttgataaact tccaggattc ggagacagta
ttgaagctca ggtacagaaa 133860taatttcacc tccttctcta tgtccctttc ctctgagaag
caaaatacag cagatgaagc 133920aatctcttaa ctgttccaag ccctctctga tgagcagcta
gtgctctgca tccagcagtt 133980gggagaacac tgttcataag aacagagaaa aagaaggaag
taacagggga ttcagaacaa 134040acagaagata aaactcagga caaaaatacc gtgtgaatga
ggaaacttgt ggatatttgt 134100acgcttaagc aagacagcta gatgattctg gataaatggg
tctggttgga aaagaaggaa 134160agcctggctg atctgctgga gctagattat tgcagcaggt
aggcaggagt tccctagaga 134220aaagtatgag ggaattacag aagaaaaaca gcacaaaatt
gtaaatattg gaaaaggacc 134280acatcagtgt agttactagc agtaagacag acaggatgaa
aaatagtttt gtaaacagaa 134340gtatctaact actttactct gttcatacac tacgtaaaac
ctactaagta ataaaactag 134400aataacaaca tctttctttc tctttgtatt cagtgtggca
catctgtaaa cgttcactct 134460tcacttagag acatcctcaa ccaaatcacc aaaccaaatg
atgtttattc gttcagcctt 134520gccagtagac tttatgctga agagagatac ccaatcctgc
cagtaagttg ctctaaaatc 134580tgatctgagt gtatttccat gccaaagctc taccattctg
taatgcaaaa acagtcagag 134640ttccacatgt ttcactaaga aaatttcttt ttctcttgtt
tttacaaatg aaagagagga 134700caaataacat ttctctatca ccgacctgaa actctacagt
cttcagagaa tgaatggctt 134760gctaaaagaa tgtcaaatct tactatacag ctatttcata
ttacactact aaatacacta 134820taaggcatag catgtagtaa tacagtgtaa aatagctttt
tacactacta tattattaat 134880atctgttaat tccagtcttg catttcacat ttgcaaaacg
ttttgaaatt cgtatctgaa 134940agctgaatac tcttgcttta caggaatact tgcagtgtgt
gaaggaactg tatagaggag 135000gcttggaacc tatcaacttt caaacagctg cagatcaagc
cagagagctc atcaattcct 135060gggtagaaag tcagacaaat ggtaaggtag aacatgcttt
gtacatagtg agagttggtt 135120caccctaata ctgagaacct ggatatagct cagccagcgt
gctttgcgtt caagcttacc 135180agagctgttg tatgcctgtt aagcagggca tacagtcatg
aggctcttga aaaatcttaa 135240cagacaaagg gcaatggaaa atcggagtta agggatggta
gggataaaat gcatagaaag 135300aggtaccaca attttgattt ttgccctaat gcctctctgc
gtggttcctc aatttttcta 135360cttcattcct catctcctca gagcattcct ttccctcatg
cttgaaacac agatgaaaga 135420ctgtgaattc taactgagat gaaaacatcc acaaccacac
aacctctggt gtggagtcac 135480attctgtgaa ggcaaaaact aggccacgta atctatgtgt
gcaagctacg tgtaagctat 135540gtgtgtgaca ggacaatgtg aggaacatac tatgtgcaca
aggactgcag aataaacagg 135600agcaaagttt ttgaagaaaa cagagtaaaa tcctgttttc
ctcttttgtt acattcttta 135660catatatctc aaatttcctc tttggttaga agcaagtaat
atttatgttt cttggtactg 135720tttgggttga agaccattct gggataagag aaattccagt
ggttcttccc ctaatcataa 135780aatgtacagg tttagttttt ttgtaacaca gaaatctctt
catcttttat cttttgttgt 135840gattctttat agagagagaa acaagactta ctgacaatag
cagcaagaaa atcaatcttg 135900gaagaacaag attgcagttg caaaaacaaa ccaatgtcct
tgcccctaca tcctcttccc 135960cataaattct acattctcta tctaccttgt gcttgccaac
atgatatacg taaactctct 136020tttcgtattc attcttaaag gaattatcag aaatgtcctt
cagccaagct ccgtggattc 136080tcaaactgca atggttctgg ttaatgccat tgtcttcaaa
ggactgtggg agaaagcatt 136140taaggatgaa gacacacaag caatgccttt cagagtgact
gaggtatatg ggcatacctt 136200agagatgtaa tctagaattt atgaagagag tagacatgtt
gttatatgaa cactgcatta 136260gcgtatctgc tcatttgtct gcatctcttt cagacactgt
gttaaaagca gggaattttc 136320cttatgtctc tctcatcaca atattcctga cattgcaaag
ctcctgagaa ataacttcag 136380attcccactt ttcctaggaa ggtcttcctg gatgagaaca
atcaatcatc ttaactgtaa 136440ctagatattt ctgcatctaa gaataatctt tgttaaaact
atattctctc tctctttttt 136500tttttttttt ggttctccag caagaaagca aacctgtgca
gatgatgtac cagattggtt 136560tatttagagt ggcatcaatg gcttctgaga aaatgaagat
cctggagctt ccatttgcca 136620gtgggacaat gagcatgttg gtgctgttgc ctgatgaagt
ctcaggcctt gagcaggtat 136680ggccctagaa gttggcttca gaatattaaa aacacatgga
aatttagctg ttgtaaagct 136740cttttcaaca cagttatcct aaaacattta accagcacaa
atttcatcat gattcaatat 136800gtgattgttg catagaagtg tagatttgtc ccactgggtc
ctgcaatagc ccatgctgag 136860catggcttgc tgaaagaact gctttagagg gtgaaaagtt
tgacacagca gacaagatga 136920ttctcaccta agcagctgtt actgtagtgg cttgaactct
aaaggtcttg tatctccatt 136980cctgtgcact gaggagcttc ttggaaagtt catataaggt
ttactagttc taactattat 137040ctcatttggt ggcactcaat gtgctttgtt cacgtcttca
taaattaatc tatctaaaaa 137100ttggatgtgg ttaaagcaat ttcagaaata acatgtacat
aatgtacaat tattgatatg 137160aacagaacac aggcatagca tattgtaatt aggaggactg
tagttatttt gaataggaaa 137220cacaatgtaa taaatgagaa ttcattgaaa tgttagtatg
ctaactcaat ctaaattata 137280aagataaaga ggcatttaat cacagctaga tttccatcac
ttgtgacaga caggcatatg 137340aatgattatg tacagctcta ggaaaaaaag tatgtaggaa
aactagtaca ttttgattag 137400aaagtctgaa aatgaggtgc cttgatcaaa gagaatacgt
gtgtttgaga aaaaaaaagt 137460ttggatagag gtggtaagag agaatatatt gaaatggtgt
ttctacaaac tgccatggcc 137520agatttgtgt aagagacatt cagtaagtag gcaaggaaag
aaatattact aggtacaaag 137580caacattagt aataccaaaa gaaaccaatt attccagatg
ccaatctcgt aatagggtta 137640agagatttcc acccctctag tagtcaccag tgcaaccagt
aactttgcta atttacattt 137700tcttttttta aatggcagat atagctttga actgagtgat
catgaactgg tactgtgtaa 137760ataagatgga agcatacttg ggagctaaac ttctagtttt
taaaaactca aattctcttg 137820aaagatcagt tcccagtcta gtaacagctg atagtttaag
tatcagtaat tggctaccat 137880taacaactgg ctcctgagag gtcttaaatg tagagacagc
tttaaactca aaagcacaga 137940gtgattttta gaatagattt cccaagcaaa gaaaataaac
agggaggagc tttaagggag 138000tagccatctc attattatta ttatttaaag aaatggcagc
aaggctataa aagaaaaata 138060agacagagca gagaagaaag agtcatggta tgcttttcta
tcttagcaaa attaatctct 138120acatgcctag gaaaaagcca tgacaagagc aatcagttca
aaaggtgtat gcaaaaaaac 138180acataatagt aactagtact gcattgccag gaaggaagtt
atgtcgccat tccatggatc 138240tcattctcat ttccttgcag cttgagagta taatcaactt
tgaaaaactg actgaatgga 138300ccagttctaa tgttatggaa gagaggaaga tcaaagtgta
cttacctcgc atgaagatgg 138360aggaaaaata caacctcaca tctgtcttaa tggctatggg
cattactgac gtgtttagct 138420cttcagccaa tctgtctggc atctcctcag cagagagcct
gaagatatct caagctgtcc 138480atgcagcaca tgcagaaatc aatgaagcag gcagagaggt
ggtagggtca gcagaggctg 138540gagtggatgc tgcaagcgtc tctgaagaat ttagggctga
ccatccattc ctcttctgta 138600tcaagcacat cgcaaccaac gccgttctct tctttggcag
atgtgtttcc ccttaaaaag 138660aagaaagctg aaaaactctg tcccttccaa caagacccag
agcactgtag tatcaggggt 138720aaaatgaaaa gtatgttatc tgctgcatcc agacttcata
aaagctggag cttaatctag 138780aaaaaaaatc agaaagaaat tacactgtga gaacaggtgc
aattcacttt tcctttacac 138840agagtaatac tggtaactca tggatgaagg cttaagggaa
tgaaattgga ctcacagtac 138900tgagtcatca cactgaaaaa tgcaacctga tacatcagca
gaaggtttat gggggaaaaa 138960tgcagccttc caattaagcc agatatctgt atgaccaagc
tgctccagaa ttagtcactc 139020aaaatctctc agattaaatt atcaactgtc accaaccatt
cctatgctga caaggcaatt 139080gcttgttctc tgtgttcctg atactacaag gctcttcctg
acttcctaaa gatgcattat 139140aaaaatctta taattcacat ttctccctaa actttgactc
aatcatggta tgttggcaaa 139200tatggtatat tactattcaa attgttttcc ttgtacccat
atgtaatggg tcttgtgaat 139260gtgctctttt gttcctttaa tcataataaa aacatgttta
agcaaacact tttcacttgt 139320agtatttgaa gtacagcaag gttgtgtagc agggaaagaa
tgacatgcag aggaataagt 139380atggacacac aggctagcag cgactgtaga acaagtacta
atgggtgaga agttgaacaa 139440gagtccccta cagcaactta atctaataag ctagtggtct
acatcagcta aaagagcata 139500gtgagggatg aaattggttc tcctttctaa gcatcacctg
ggacaactca tctggagcag 139560tgtgtccaat ctgccgctgc cctgatcctg gctggggtga
tgggacagac cttggctgcc 139620actgagacat ctgagacact gagatctgtc tcaactcaga
tttacccaag aacagatcat 139680tgccaacaga acaaaatctc aaacttatgg ctagtgatga
cagcagtcag ttgtcccatc 139740tgtgacccac caaggctggc atgctggaat gagcaggctt
tggtggcttg tagttactgg 139800acagcaccac tgacatgggc aggggaaaaa ctgagcatgg
tgtaaatcac tgcctcaaag 139860ccacttctct gtgcctgcac catgcttgaa agctcttcta
caggagctgg gtttgttcaa 139920gaaagcttct gtttctccca tctgcttctt gtaccttcac
agggacagag ttagaagggt 139980acagccatgg ctggaagggg ctgactttca aatgtgccta
attttccttt ggttgctgct 140040gcagctgcag aagaaggggt tcagaagcca agagctttga
gataaggatg cctaacctat 140100gttgaagaca tttgtgctga cacctcaggc cccaggatag
gacaactgct ggattgtggc 140160taacccacta gctacagaac ctaatttata ttaccagatt
aggaagagca aaagaacatg 140220tatttataac aggaggtctt ctgtgcttct ctactaaaag
gtgctgtgaa ggagcccaca 140280gtgcagcagt gtatgaggcc tgaaagaggc cgcagcacac
gaagagccct ggtaggagca 140340gcacacagag gggcaggagg gctgggggaa ctgccaccca
tggggacctg tgtgaagcag 140400tgcactcctg aggggtggac tgcgtgggaa aggaaaagaa
agcaaacaga cctgtgatga 140460actgtcacac agactgcaga gtgacagagg agggcttgag
gcagtgcgct tactgcaggg 140520agtggcgctc cttcctcaca gcagcgctaa cagcttggca
ccaatattca gtagtctgtg 140580gtgatgcttt ttccagtttc accacacagc atttcgcttg
ttctacttgt tttagctttc 140640cccctccaca agataacaca tactttgcca gtcagtccct
aagaccttag cctaacagtt 140700agcaaacagg atcttgcaaa agaaggaaga taacatgaca
ccaccttcac tggtgtataa 140760atagttcaaa tactttcctt cactttcccg taaattagtt
gattgcaggt caggagataa 140820caggggaact tactgcaaga gagaaaatga tgtttaatat
tgtcttggac tttctggtgg 140880tctgggcatg aaaatggagt actcaaaatc ctcaggacgt
ttatttttca cctgatttat 140940tcccaaactg cactatttct aggccattgg agttcttatc
aattaaatta tactttggct 141000ctctgctatc tcactccctt tcatcttcag catcactttc
agcacaatta caggagaaga 141060cttagactca gagctttagg actcatcata agaggctttc
attgctctgt caccacaccc 141120catatagatc tgtagtatac cacacatgtg aagaagcaca
gtacattagt gcattacaga 141180gagacaaaac cacacctatt tgtgtgcctg cagtcttaca
ccagcaggaa gataattaac 141240gtaatgaatt tctataaaaa tgagagaata tggcccctgg
gtcctactgc ttgttctagt 141300cctgattctt caaacgtaag aatgcaagta aaattactca
cttgaacaaa gtcagcaatt 141360tgcaagaact gatattctga agttcaagta attagagtga
tttccagtac ttctggctgg 141420aacgggcagc tgaaaatcac ctggtccagc accttgctca
aagcaggact atcttcaaag 141480ccatatcaga tagctccaga ccttccctag tcaagtgttg
cctatctgca tggttggaga 141540acccacagcc ttctgattaa tttgatttta aacataaatt
caaatgtcac tagcgtagca 141600gtagtgaaag ccattcaact ggctttactt tctcttacca
aatgagagtt agctgcaggt 141660gaaaataagc cctgccagtt ctcatttttt ctcccacagc
ccacaaagct ctcactgtct 141720gtcctcactt gtaatacttt tgaaccaaca tctacagatt
atctctgtaa atcccaagca 141780gtacctagtc accacgtgaa caacaaattc ctacatttaa
caatatttaa gagcaaaggc 141840cagaccatat gtagctgcac actacacatt tttagaccca
atagtataat ttatactttg 141900actccatgtt gctgccatgt ggataacaat gcgcaatcat
ttgtacctgg cttccttttc 141960taactagtat actcttaaac gtcacaagat aaagactcta
gttctgtata gtctagctga 142020cttgtgacaa gagcaaacac tcacaatttc atggtactcc
tgaggaaaaa aaggatccca 142080aactaatttt gagcttttac atattttttt ttaacctaca
gagcaccttg ctacttctgc 142140tgaatgttag caatagcaac ccacagtctg aaatcaatgc
aatgaacttc tactatgggt 142200accatactga tgacaggaat agtgcaagtc cttacactgg
aaggctgact ccttagtcac 142260ataggtaaaa tttagaaatt gcagctctga taagagatca
gtatgggaaa gggaaaataa 142320tggggtgcca gatgagtgca ccttcctgaa aggaaggcag
atatatggga attaaaggtg 142380gacaagggat gctgtggagg taccatcaac tttcacaggg
ctgtatgtaa aagcagctct 142440ctttcctgtt gattctccgc tgcctcattt cttctgggca
aagtttgtta ctctccagta 142500acgtcccttc ctcaaactgt tacctaatcc caccctcatt
gccttctctg ttttgctctg 142560tccttcagca gtctctacct gcttcttaag gtagtgaagt
aagagggcag ttctggagtc 142620aagctctgtt tctatgaggg taaaggccag ggagagaaag
gtttgggagt gtgaggagag 142680cctttttcct gtgttgttca agtacttagt ccaagctgct
ttcagctgca tctgcagaag 142740atggggaatg gagggtgatc aatgccattc ctccagccac
agagcaaggg ctttgcctct 142800ccttgcatac agtatactag ctttccttag tcaaatgttt
cctctgtgct gcagagtcca 142860aggtaaagag gctttgtcta cagctaggtc tatgttccta
gagaaacaat tagcaactgc 142920aaaatcaaga ggtactaaga aagcctctga agctataccc
aggggtctgg caaatgaagg 142980gggacagatc aagaagaaag aagagtctag agcagtttaa
gggaataatg ccactagttt 143040taagccacac atctggtggt aagcttttaa ctttgaaaga
gacagaaatc tcaagataca 143100ccagcccaaa atataatgga gccataaagg tctgcacgta
gctgaatccc aactggaaag 143160aacagcttca aagagcttgg aagtgctgag gtgaagaaga
gcatgtgatc attagatttc 143220aaaagaaggt cctcagcaca ataaccagaa agttcacctt
tctgtgggac aaaagatgcg 143280tccctcacaa aggctggggg aacaaaatct ttgcatctca
ttttgcctga gaggagaagg 143340aaatacaaga tcatcttgtt ttacttggtg tgtatcacat
cattaatttc tatttggtca 143400ctactatgca gaacttgcta acttgaacca tgtaaaaagc
acactaggtc tcaagagact 143460aaaatgcttc ttgcaacagg cagagtgtga gagatggaag
gatggaaaaa tcttgcagtg 143520atgaaggcac tgataagaga tgttgaaatg atactaacaa
atggcactct atctttccca 143580agatctttgt cagcatgaag ggaaaattct attccaagct
ctctttgagg ggttaccatg 143640ttccaggata aagacttgct gcatacacaa gcgcacttag
tcaggtcact cagatcagtc 143700tcatgctaaa aagtgtgaaa atagaaatac aaataagggg
ccaagcagat tactgaacag 143760caaagattgc cagtacgtgt ccacaatgag tatttggaca
tttcactgcc gaaacttctg 143820aaaatatcaa ctgccttatg aaactctggt tattccaccg
cacaggagta tttgtggttg 143880agctgcatga agaaatagca agtgtttaaa ctgatttctt
aaaagagagc ctttcctcta 143940catgctgctc ttgcacatcc atgcgtggct cctcttcagg
agcaggaatt ggttttctga 144000ttcagcagtt gtgtagctga cgtagttata ccctttgaga
gatttcttca gaaaaatgac 144060atgtttaggc taaagtgcat gtaatccaca catacaccat
tactcacaat gaagtactat 144120gcagcatgaa attcaggcta ttcttcttca tatttttggt
tttaattgct accttggtta 144180cttaaaaaat gctcaccatc tgattcatgc aaaggaaaac
tgcacactgg tagatgtgag 144240aacagcacgc atactcactt ccagataaac taatctctac
tcagatatcg agatcattgc 144300ttctccagaa gtgttgcact ggtcatcaga actgagtatc
tcaggaaaag cactgtcttt 144360tctaattacg gcatctaagc taaagcacac agcggtaata
gtgcagtatg acaaattatg 144420ccagtgttca attcatgtgc caaatctcac cacgcctttg
cgttctgcag gtgtggagca 144480aaatgcctca gtgatattta gacaggaaca ccaccacacc
tcttaacaac tcataaattc 144540taaatgctat tggagtatgt cagcaaagat tgcttggcaa
aggttgcaaa tgtacatgta 144600atatgtacgc tttagatagc tatctacact gtttcaaaat
aaagacgcgt gtgttctcac 144660tcaaagcttt aaagggaaat aagatactca aagaaataat
ctcttttgaa ctttaaaagc 144720tatttgagac ttcacgatga tacaaactta tcccacataa
aaatcttagg acataaaatc 144780cattacaacc attccagctg agacatatac accattgtta
cgctttaatt tacaaggtca 144840ggacaagctc ttgctgcatt ctgtgacaaa agggctcctt
tgcacaccaa aatccatgca 144900cccactccaa gcacctgatc actgatcacc attaccatca
cttcagtctc cgtgctccca 144960ttccccatac tgttttggct cttgccaatt acaggattgt
tatgaaacta aatgttaagc 145020tgccctccca caggattcca acattctcag gtttcaaaac
cattgtcttc cccacccctc 145080ttatctcctg aagtccttat aatggtttgg acatttaaag
tcctttcatg tttaaaactt 145140actggcctgc tctggctgag acaaaaacac gagcagaatg
ctctgttggc tgaaccagaa 145200accattcccc cccagataaa taaacagcac ttttactggt
aaaaaaagat attagaagat 145260gccaaagaaa tggagtagct tttcttcaag cataattttt
ttcttttcaa ataccaaaca 145320ccttaggttt gaattacatt agattttcaa gaattacaaa
gggttcgtag ttaaaacagc 145380atacgtacat gaaaaccagc catggcaagt ttcacacaaa
tactgtgtga aagcagaagc 145440taccaaacct tcctctcaaa accctcaagt acatttagat
cactttataa atgatctatg 145500tagacagcaa gtatttaacc tactcctgat cccaggtacc
aatgaactga gcaacatact 145560gtgtaggaaa gttgcactga cttgtgctaa gttgcacgga
aactgaagga aaacaaaatg 145620tgcttatata gctgagatct ggccagggtg cctggtgtgc
tgccaatatt tgtcctgcca 145680aaatggaaac atgaatgacc acagtgaatg aactacaggc
ttacttccca caggaaggat 145740actaccaata caaacataag actttgagca tgttggagtg
ttgacttagt agagagtggg 145800agtgagggaa ccgctgctcc tgagtcagcc tcagcaccgc
ccattgaact ctgtacctcc 145860tagccttggt aacttcacag gatgctggaa aatattatca
agtcatcaca ttgatttatt 145920gaatatcttc cttttagatt ttagttgctt tgtatgtatt
tttttttcca ctaataacca 145980gccatgctat tcaaaaggca tttttaaaag gcaacgttaa
caccctgtac aaacaccatc 146040ctctcattca ttcaaatccc acatttctgc atatatggaa
catgtcagtc acttcttgta 146100acagagcaag tactatgact agtcagcaaa ttaaattcat
ccctgcttta aaaacagaaa 146160atccaagtaa ctgctccaag ggatgaagtt tatttagtgt
atctatcatt tgttctacaa 146220cacagttaat tttgcaaaga tgactcaaat catttaaagc
tttggaaatc atttaaggcc 146280aatgtaaaca gattacaact ttcccaggcg caatggaagc
aattaattct gcagcacacc 146340tctcctacac tactatactc tggaaaacgt aacagatgca
tctaattata acccacactg 146400aaacatgctg tctttatgta gctatgaatt caaaacagct
gaggggcagg aaagaaccat 146460cctcctaaag ctatgtggct gctcacctgt aggaaagcaa
cttcagcaaa gctttgagtt 146520cccaggttac actgatgcag aagcttcacc actggcaagg
tgctccttgt gtgagcaacc 146580tattctgctc tataaaacat ttagcagata atcccatatc
tcagtcctca gtacaagaga 146640ctctgtgcca gccacttctg tacgaaataa gcccacacgt
actttcatag acctcagggc 146700agaagaaaag tttcagaaag cagtttgtgc tgaggagata
gaccttgggg gtgagtcttt 146760ctccatattg aggcggaatc cctcaaagac aagcagccct
tatccaggtg ttcaaggtga 146820tattttcaac agagcaggga ggtaaagaat gaaataaagg
gcagagttac ataggatttt 146880tcagtcagag gtgagagctg agatggacag gacaatgagg
taaggacagt gtgactgtga 146940ggagaattag cgatggaaat gcttcactag ccaaggcaag
aagaaaaaga gtattcaata 147000gaatatcaat ttctggggaa agaattcatc tctgaagggc
tacatagggc aaatagctgc 147060tttcaactta gaacagggaa actgaggcag cagcaaaaaa
aaaaaaaaaa gtctaatctg 147120aaacccacat caggttctac tgttgttgca gtgataagaa
aagtgtctga tgagtgtttc 147180tcaaccttcg ttatctcaca gtgaaaacat tttcctggtt
atacagtttt agaatcctcc 147240aatattacca aaaaatcatt ttactaaaaa tggaatccca
caagaaatga ctaatttttt 147300atctgtagga aacggacaat agaaaaactc ataaatatga
tgtcactgtc ctttcgctgt 147360ctcttccttg gaaattgttt ctattagagg aatcataagt
aggtcagcta ctgcattttt 147420ttaccctcca aattgcaaaa gaaatgttgt ttccagcagt
gatggttcaa gttgtaacta 147480gcctgttgcc acaaaaatgt ttatagaaat atttctgcag
tcagttttgt aaggttcttg 147540tatggtatca ctctcaccgt cacttcacat cctactctga
gatgattcag ttcttccata 147600gggatgtggc cttcagggca aaataaattg cagagtcatg
agtcataaga acctttgaag 147660aacaggccag gcagactata aattcagcct acccatcatc
tgaactgcat aaaccttgga 147720cagacccaga gcagcagtct tcctccctgc acaaaacaag
gtactgtaat tatttctaga 147780gattatttat ttcacctact cttggatgat gtcgatctgt
tgacaaatgc atagaaaaaa 147840aaggcagaag gaatctgaat agaaaacaat aaatacttga
ggaagaaatt tactaagatg 147900gcacaggcaa ggtctagaag gggtaagtct cagaaatatg
caggaagagt ggtttattct 147960atgtagttct cactggcaaa catgtatatc atagcagagt
aagaaccatt gtgtttgctt 148020aagttagatc atttgttcat gtgctcttca attcttgtgt
acgtcaagac acagtcagta 148080catctttatt ttatggctat tctgtatcaa ccagaatagc
tcccactaca tacctagggc 148140tctcagcttc aactgcaatg caaataacaa agagcagcac
ctgtgttcta ccaataagga 148200aatttgtctt gcagaactgg gaagctatga ttcggactag
caccataaga cagagtttcc 148260agaaattttt gaaagttaaa aatggaattc aggatactta
gcacacagta gttagagagc 148320tgcttgtcta gtgcttcaat attttcttgt atcctaaagg
aatggaatat ttgttcacta 148380cagttacgag ctccaaaagg ctcttgcacc agtagtcata
agaaacaatc tctcagcatc 148440ttctagccct cgcaccagtg agtagcatta tcatatgtca
ctccaagatg ctgtaaagga 148500cagtgcaata aacgtcctga tgaacaaata caaaatgaaa
tatgaggctg ctttttctat 148560atcacttgag tatggttagt gttgttggtg aaacgggatg
cactatactt aatataactt 148620tttagtagct aatcttcctc attttccata aagatatctc
tcttctttcc atctgtaagt 148680tccttaccat atcattatat tgcttacata gaattcacca
tatagttata aattgcatgc 148740tttttctttt tagtataaca ctgtaaccat cctgtttcga
tgaattttct tttgtttctc 148800ctggctgtcc cttgcatgga tatctgtctt tctaagtgaa
cttctgctga accaaggaga 148860gttcattcat gcactacaaa cagacgtagt tggcagaaat
gaatattgta ccagtactga 148920caggtcagaa tgctttcatt cagtttcctg aggtcagagg
agagctgaag aaaatacctg 148980gcacagtcta gattttgcct ttgaacatac actgccagtg
agcctcagtc atacaaagga 149040tcactgtgct gcactggcat tcttccacag tcaataagtg
ttaaaaatac ttttagaaag 149100cctacaaatc ataaaataaa cttcaaatac tgaaagggag
cctgcagaca tatagcaaac 149160acaattaatt cttagctaat aacatctttt gtccctttct
gcgcaggttc agaacaatgg 149220aagctttaaa taaagcaaac acaagctttg ctcttgactt
tttcaaacat gagtgtcagg 149280aagatgacaa caagaacatt ttgttctccc ctctcagtat
ttcatctgcc ctggctactg 149340tgtatctggg agccaaaggc aacactgcag atcagatggc
aaaggtgagt ctgagaagag 149400ttgatctact ggagtaacat tctctatgat agaaatttag
catgatgcat caaaggaaaa 149460ccttatgcag gtcaaaagat acagtctaca gtagcttctg
taagcaggcc cacaccaaat 149520gggagcagtg gcattagtga cattgtctcc ttttaaatgt
cattggaaga aagaagagct 149580cttaatccca aagctcaata acttgagcac tcaccagtga
gagggagact cggattctcc 149640acctgccttg ctccagaaaa ttctcatttt ctgtctcatc
tctctggaaa tggccctatg 149700cagaaagacc ctccactgta tctctagtaa gttttgtgct
ttgctaacat aaatctacaa 149760acccacaagg tcagagaaga aacacagtca gggtgataat
acaacccctt cttttgagta 149820cgtactttac aagaaaaatc acctactgaa gttcctaaac
tctgtgcaaa gttcatagct 149880tcaaaaggca gcaatgagaa cagccccagt gcaaacatgg
ttacctagca tgtactgcgg 149940gaggggctga tgaactggca tctgctaagg cagaagaagc
tgctccacct gctaaggcag 150000caatccaact actgagctac aggacaaatg aggaccagca
gggtcacaaa gaaaggagag 150060atcttctgtc aggaagaagg gaaaacaaaa caaaacagaa
ggcttttgaa aaatgttcca 150120aggttagatg tacacctctg tagcctgggt aaggtgcaca
tgcccagagg aaggcattta 150180gggtatcaat ttgctcccag tgtttacctg ctttctgaca
tgtaccaggc tctccatttt 150240acacccatgc tttggcagtt tccacctgca gataactggc
ccgtcccagg tattacccta 150300tgagtacaag agccgatttg aagcaggcaa gttcctctag
aaataccaga tatatgagaa 150360ttctgcttgc agccctcatc ttagtgtgct caagacatcc
tgtacacatg ggctcaaaag 150420taaaatctgt ctttgtctct cttcatcacc agtcccatga
cattactgaa agtttttact 150480gaaacagcaa attttctatc attgcattta ttactgcaat
ttccactgca ggtactctac 150540ttcaacgaag ctgaaggagc cagaaacgtc accacaacca
taagaatgca agtctattcc 150600agaacagatg agcgcctatc aaatcaccgt gcctgtttcc
agaaggtata taaccaagtc 150660taatgatcat agaatcatag aatggcctgg gttgaaaagg
accacaatga tcatctagtt 150720tcaatccccc tgctatgtgc agggtcacca accactagac
caggctgtcc agagccacat 150780ccagcctggc cttgaatgcc tccagggatg gggcatccac
aacctctttg ggcaacctgt 150840tccagtgcct catgatcaag ctacagtcat gctaacacct
tcccttgctt ttattttctc 150900tctctgtttg ccttcctcaa atgcagggta cacaactgat
tagtacagca tcctgtgata 150960ccttcacctt atgcaatact taagacatgc ttcccatttg
taggtagaat tgcaaaattt 151020aacctcaaat ttgcaaaatt tgaaatttaa tgatgggact
attctctatc agtggacctc 151080ttgatcttct cccttcagct ttgagatttc tctctttttt
ttttctcttt ctttttttcc 151140cctttcctct tctttctttc ttctttttct ttttctttct
ttctctcttt caatctttca 151200ttccttcatt cttttctttt taactactac tgttcaatta
gttattgcta gttattcagg 151260aacatgttct ttgaacaggc acagtcccta tctcagaaca
aatcagaaca acaaaactac 151320tctcccacac attggattgc agcatcaaca caacaacaac
aacaacagaa aaaaacagcc 151380agagaagtaa cttttacaga ctatccatgc ttgatacatt
tcagaaatta gtttctattt 151440catttgaaag tttagtggaa taaattggca tgttggaatt
tcctaaggta gaccttgcaa 151500taaatcttaa agaaatggga attattgtat tcccgagata
tttctttgac agactggcag 151560gcatcttttg ttaaaataga cataatttaa gagagcagaa
aatttggaag tcaaactctg 151620agtgagtaag gtagtttttc ctcactgaca atgcagccac
tgtggtaaaa agttcctctc 151680cctactcttt cccatcattc ttttttcttt ttgtgagtaa
atcatttccc tgaagtctgt 151740ccacaaaacc cctgtggcag caagttttga taaatgggaa
cttgggtcta cattccacag 151800ctacggtggg aagactaatt ttggggacta cgccaacaaa
ccatttatgt tgcacgaaca 151860ggagatggat tgtttctcat gagtaatgct tgtctgaact
gtaagaatta tggagcgctc 151920taggcaggga aaagaaactg ttctaatagc ttagaaattt
agatagctgt tcatgcttct 151980gattttcttg cagtaacaag atgaatacaa cacaggtcca
gtttcttagt ccactaattc 152040acagcttcat ttccttaagc tggtttgaca gtttgagtcc
acattcatat aattctgtta 152100cataaatata aagaatttac tgcaattact acaacaaaaa
gcatttgcaa aatattatta 152160tttagaggta ggttaaaaaa gttagaggca aacttaccat
gtaattaact ttcataaatc 152220ttatcaggag tcacacagcc aggtcttcat gtatagttta
gcaattacat tctgtctctc 152280tctctgtatg tacttcattt tgcaacctcc atttaaaagt
ccttaaacat tctaaacagt 152340tcaagctttt actacttgca tcccagggct cttacagtgt
ctatagcata tctgaaactt 152400ttagtaattt cacatcattc ttttaatatc tgtctgagtt
agtacacatc ttgcattgca 152460gtaaaggcaa caccacctga atagcagtag tttacataga
gctgcatgag gaaagaattt 152520agaaattttg aactgtttta cagaaaaaaa aaaaatgtat
aacccttatt tccttgtctc 152580caagacagaa ataggcaaat caggtaatat ccatgctggg
tttaaagcac tcaacttgga 152640aatcaaccaa cccactaaaa gttacttgct tagaagcgtc
aaccagttat atggagaaaa 152700gtcactgcct ttcagtaagg taggtaggcc atttattcat
gttatcctgt gtgtgtcaga 152760ctttatgatc tatctatgac aacaaaccat aaattatatg
ctttcaaata ttttcattac 152820atctgcaaat tgtgtaatta tctttaacat acttcctgtg
aggttcttct tgagaattta 152880gatatcatga cttttatagg atgtatattt aatttgtgtg
attcacagtt gtggctacgc 152940aaaaacattt aaattatgta tttccaaata aaatcaatac
tatgttcttt tgacaatgct 153000gtgcttgtag cctacacaat ttttatgcat tctctccaat
cggctatagt tatttattgg 153060cattcacact ggcaggcaac aaacataaga cagatgtcta
tcttgcactg caggaatact 153120tacagttaac caagaaatac tacagtgcag aaccacaatc
agttgacttt gtgggagcag 153180caaatgcaat cagaagagag atcaattcca cggttgaaca
ccagactgaa ggtaagctct 153240agcatctcct ctcccagttc tgaaggaagc agttttagtc
ttgaacaatt tctctgtgcc 153300caaaggcagg taaacaattt aactcagaaa ggaaaatcag
aacagttttg ctgaagtaat 153360catctgctgg caagcccttt ctagaattat ctttcaccat
ttgaaaggga gaggaatgtg 153420gtttcctcta taaatcaagg ttgtcatgta tttatgaata
atctcaagct agaagtatgc 153480caaatcagca ctctaaattt ccttgtctta tgacttcaga
aactacgcca gcatttactc 153540tgaaacagta aagctgcaca aatatgtaaa cgttctttgt
ttttctctag gtaaaataaa 153600aagtctgctg cctcctggat ccatagattc actcaccagg
ctagtcctgg taaatgcgct 153660ctatttcaaa ggaaactggg caacaaagtt tgatgctgaa
gataccaggc aaaggccttt 153720cagaataaat acggtatggt aacatactgc cttatatacc
agactgcagg ttgaaaaagc 153780agtgaaaaag atggaggaga taaattcctg tcattcttta
aagccacata gcactaaaat 153840tagtatattt aaaacatacg ttatatcctt cttagcacat
cttcagtaca aagaccgcat 153900acatatgcta gcacccaagg cacaaataaa attatcagaa
gccagcttga aacaaacttc 153960catacacctc ttaaagcagg aaaaacatag atgtgaatag
aactgtatga actagttcta 154020tatattttca tttttaacca tacaatgaat tggagtggaa
cagagcttcc agtaaatacg 154080tgtcatccta gctggctaag ataaccttcc cagcctccca
gtgcattccc agaagagagg 154140ggccctctgt agatcctaca gcttctctta gagccacagg
gatgtacctc catgctactt 154200caatgtagtc tttactgttc tgagtataaa tagcaagctt
ttcatttgat ttgttgcagc 154260atacaactaa accagtgcca ataatgcacc tgagtgataa
atttaattgg acctacatag 154320aatcagccca gattgatgtt cttgagcttc catatgtcaa
taatgaactc agtatgttca 154380tcctgctacc acgggatatc actggcctac aaaaggtaaa
gggtaacttt aaactcaaat 154440tgcgtgagaa acaacgtttt catgcatatc catggcaaag
caatcctgtt tctaggaagg 154500aaggtatcga taaggctaaa ggaaaaacaa accccaaact
tgcccaaatg ttatgaagct 154560gaaccttttc aatgttttgt ttggttttct ttttaactcc
tggcacgtgg cacctcgtgc 154620ttcctcatgt tgatcagtgc tggaaataag tagcccgaat
ccaacaagat agatctaatt 154680ccagctgaag aacaacgagg acagaaagat agttctgctg
actgtctgta ctgattcgga 154740cagatattat tacattaaaa agaaaagcac aaactggaca
ccctccacta ctttctgtga 154800tgtttagagc taatatacat gtacactgcc accttctgta
aacacactga acctgacttc 154860agatagtgaa ctactgtgaa attctcattt acattagtgg
gtgttttgta gaaaaaaaaa 154920aaaaaagtta ttttcactaa attctaagac acacagaaaa
cagaaatgtg agcagcaagt 154980caaatagact attgttactt gacagtgacg ttgttttaca
aatatttaat ccctctatat 155040tccctgatga ttactaagaa cagttcaaat actgcactaa
catgctgtag agcaaaacac 155100tccttcctag tagaaaatat ttcagagttg gcatttcact
aatggtttct gtacttgaaa 155160agtacaattt tttttgctac aaaaaaaagc tacagaattt
ttgtagtttg aaaagttctt 155220aaataagaat ataaaagaaa taacccctag ggaacagttt
tttgaacact ctgtaatttt 155280ctggttctct tttcaattaa ctgcagctaa taaatgaatt
gactttcgaa aaattgtctg 155340catggaccag tccagaatta atggagaaaa tgaaaatgga
agtgtatctg cccaggttca 155400cagtagaaga gaaatacgac ctgaaatcta ctttgagcaa
gatgggaata gaagacgctt 155460tcactgaagg tcaagctgat ttcaggggaa tgtcagagaa
cgctgacctg tttttgtcac 155520aggtttttca caagtgttat gtggaagtca atgaagaagg
cacagaggca gcggctgcca 155580gttcagcatc tctagcgtca cgaacccttg gtgctacagt
tatttttgta gcagatcacc 155640cttttctctt cattatcaga cacaacaaga ccaagtgcat
ccttttcttg ggaaggttct 155700gctcccccta gaaaatcagc tattaataaa caagccctta
caacaacgat gaacacaatg 155760tatgccatga agaacacctt gacagacttt gcactttacc
attttcctgt actattgaca 155820atctctttta gaagagagct caaattaaaa acatgaattc
aaacctctga ttccttttcc 155880tctgcaaaga atcctagcat cgtatactgc actgtagaac
actgaactgc acgctgaaca 155940acatggatgt gtcttttcag tgctgtccaa accagaactg
ctacaatgca gaacagacta 156000ggctgatcta aacagtacct tctgacccag ttcctttcac
acgtaagaag aaaagaaaca 156060ggagaaactc attcctgcat acagctgttt catctcttca
aagccagctg tcccaggcca 156120gctcaatcac agccttgtca gttttaaatc agcttcacaa
catagcatgg ctggtaatga 156180aacaaaagtg caaaatcctc tgtgttgctg atactggtgg
tttgctcttg cacacaaagg 156240agctaacaca tgtactttct aatctctgtc cctcataaac
tagcaaatac caaacaatac 156300agaaccagag taaagtaaaa tacatacctt gaaatgcttt
cttttgtcat aacctttaat 156360tcattcaacg ctgttgcagc ccagcactgc actgctttac
ttgcctttta ctttgccaca 156420tattttgctg cttggagcaa gtgggagaat aaagtctgtt
atgttaactc cctaagtgct 156480gtctaaaaga ttacatgcaa attctcctct acatattcac
tgctttcaca gcttttactc 156540ctaaagggga ggaattccta atcagtcatg cacatctaag
aacacaggtg atgctcctgt 156600ttctctgaat tcagaacagg gaggaaagga ctgggtctct
taacagcact tgcacacaca 156660ctgacagcat ctcactagaa acatcccttc ccagaaaggt
aggatacctt tttcctggca 156720gagggaagag cgctgactga tagtgagtcc tttctgtatt
attccacgtg accaactgtg 156780gccaggctcc cttttggctc tgcttcccaa atgggaagga
acgtagggaa gggccaatgg 156840caaccaaatt agagggtgag tcttgcattg aggaacacca
ttttcccacc gtaagtagca 156900cagcactggg gcagactgcc cagaaaaaaa ttgaggattt
cccattcttc aaagggctgt 156960agctgaccta atttaccagt gggtctgctc caggcatgag
ctggactatg gaaataccca 157020taaaccagct tgtgtcttgt ttctatcaac atccattcta
ccttaccacc tcaatttctc 157080atcctctctg gcacccttac agcttgacaa gcaggggcag
ttagcttgct tgcttgctct 157140caagcatatt tctttgagac ttggaatctc cctagcttgt
actttcccat caatcaatca 157200ctcagtggac ctttcttcta ccctgatttt ttgacaactc
ctctcattgt actaaagtca 157260ccacttgttt agtttctggc atttcttcat tgccttgcat
aatactgatt tctataactt 157320ttcagaagac ttgacccgct ctgttcttgt aatcaagctt
acagcaaaat gcttggcaca 157380ccatacagtt tccttccctt cctcctcctt ccttccctgt
cccctagact cttacagata 157440ttgccactac cttccccttc tcttaccacc aaagagctgc
tctgctgtct tctgggacag 157500cagagtgaca actattgtga catatttatc cctgttgtat
tgttttcact ccctccttgc 157560tcaagctctg ccagtggggc cgtttcagtt cttgcccacc
tccatggcat gcaagtactg 157620cacactaact cagtactctg cagtgcttct ctgaagcgtt
tcccagccat ggatgcaatg 157680agattctttc accaacacaa gaagcaaggt attcaacctt
acaccttcaa tggctttgcc 157740tctgcctatg ctcctaatga tcttcactct gaagcttgac
tctcagagtc tcattcagtc 157800agtaagctcc tgacatttta tgctgatgca tcttactcat
agaatcatag aatggcttgg 157860gttagaagat catctagtcc caagcctcct gccaaccact
aaatcaggca ctacatcagg 157920ctgcccaggg ccccatccag cctggcctcg aacatctcca
gatctccagg gatggggcat 157980ccacagcttc tctggggaga ggttccagca cttcaccacc
ttctcagtga aaactttcct 158040caacatctaa tctaactcta ccttatttta gttttaaaac
attccccctt aacctatcac 158100taccttccca tgtgaaaagt tgatttccct ccttacttac
aagcttcttc taggtacttg 158160aaggctgcta tcaggtctcc cctgatcctt cacttcttca
gactgagcaa gccaagcttc 158220ctcagccgat cttcagagaa gaggtcttcc agttctctaa
ccatcttcat ggccctgctc 158280tggacttgtt ccaaaggtcc acatctttcc tgtgctgggt
gcctcagacc tggacacagt 158340actccagatg gggcctcatt tgggcagaac agagggggga
caatcacctc cctctccctg 158400ctggtcaccc ttcttttgat gcagcccggg atattgttgg
cctttcaggc ttcaagagca 158460cgctgctggc tcatgttaag ttcttcatct gccaggatcc
ctaagtcctt ctcagtaggg 158520ctactctcga tgaattctta tcctagtatg gatacatatc
tgggattgtc tcaacacaag 158580tgcaacacct agcacttggc cttgttgaac ctcattatgt
tcacatggac ccaatcctca 158640agcctgccca ggtcgctatt aaaaaaaaca agtgtcatgg
cttcacaagg ctggaaagtt 158700ggatcaggcc tacaatacct gctaacatcc agaaaccaaa
accatgcatt ctggctctgt 158760aatcatttta ctagatttat ttagtttaaa caacaggcca
gttgtcttca caacaacaga 158820agatcactga aatgagtgag tgacttgttt tactgtcctc
tcagtagaca attgtggtgt 158880aacagttaaa atgagattat gatccacatt gttcctttga
aacgcctaaa actaaaacat 158940aacatgatga acaaggaaga caaaactgta tgagatcttt
ttgtgattca ttagaagctt 159000tgagtaggcg ggaacagtgt tgacatagga gagaaggaaa
aggaagtgca aactgtacta 159060tatttctaaa tttattcact gcataacaca caggcacaga
acctgactga ggacaagact 159120caggtcttct ctctcacggg accacaggta aacatttaaa
ccaatttaaa taactttttt 159180gatgttttta aatggttatc tatagcttgt atgacaatgt
aagtatatta aaacacacca 159240gagttattct gtagtcggga gcataattga tcacaagaag
gaaaatcttg tcaggacagt 159300agctgtctta ctaattaaaa tgttcagttt gataaaggag
tctcatactt caggtaaagc 159360aaaggccatt ttcattctgc cttgtatgag gtcaggccag
ggactcagag gagcagagta 159420aaacagacag atttctatcc tgatgctcat ttggtcaggt
tctccaagga gaggaagtca 159480ctctgttggt acagatttgg tgtagactgg ataacgactg
ccagaaaaac tgaagtggtt 159540tggtaaccaa aatcttgata aatatctgtg gacttcagag
attgtcctgc aatttctgca 159600gtgccattca ataaatataa atctttcttt acataataat
aactactaca acaacaacat 159660tttccagtcc ctctatcaga aaacaacatc aagaaggcac
tactgaacag gtaagttaaa 159720gtttggaatg ctcatagctt atatgcatag gtatttgcca
gtttctgggg aaaataaaat 159780tgcaagaata taaagaagag attgtagtta gacttcgtga
ataaaatggt aacactctaa 159840aagcaaataa caactttgcc atacattata ttatctgaaa
tgggtgacta gccagaaaaa 159900ttccataagc ctaagagtta cacctaaata cattctcagt
atcagctcct aattctatct 159960agatccaaaa tgaggtagtg aaaagttcaa atgtcccatg
tacaaaaaac tacttaaact 160020tccctaggaa cattactttg ataatgagtt aagaatgaaa
atgaacaaaa tatgcagctt 160080acaaatccac acacttttga aaaccaaagg cagaaagaaa
cacaaataaa agggcagatc 160140tataaaagag gacatatcta taatcataga gaaatatgag
atggataaca aaaacctaaa 160200agaaactgct gctcccagca ggtggcacat ggtatgtgta
gaacatataa cgtacaacta 160260ggctattagt ttcaaaaggt acctacgtgc tccgttgcaa
atgtaacatg taaatgtaaa 160320atgtaaatgc aaatgtaact aatatgcact acatacatca
ttttagacac tcaaatacta 160380caattctgtc tgttgcctct ttccaggctg tagcaatgga
acaggtctcg gcatcaattg 160440gcaactttac agttgatctt ttcaacaagc tgaatgagac
caacagggac aaaaacattt 160500tcttttcccc ttggagcata tcatctgctc tcgccctgac
atatctggct gcaaaaggca 160560gtacagcaag agagatggca gaggtaagta gctctgtgaa
gctatgatgc tcaacactgc 160620ccagcactgc tgttgagatg ccctgctccg ttgtcatagg
gaaaaactac atttgagttt 160680gcacaaatgc attgctattg ctgagtgcaa tggctgtgga
agggatttca gccttgtagt 160740gcacagacag aagcactgtg atgatgctca caggcaggag
caatactatt ccttgttact 160800gtaggggatt tacatatact agagctccag tgtccctctg
attagatcag aagatagcac 160860agtgtgttat cataaggatc caaacaagac aaccatttta
tctcttttag gttttaggtc 160920atgcaaactc tttcatgtca gtttcttacc tttggaaacc
ctgtttgcag gttcttcatt 160980tcactgaagc tgtgcgagct gaaagctctt ctgtggccag
accttctcgg gggagaccaa 161040aaagaagaag aatggtatct attaaacatg aaatcccaag
ataagagttc aaatgtctgg 161100atatagtttt taagagtcca ccatttcttg tttgcagctc
tctttatgtt taaagtataa 161160aacccaatat acttcgcatc acatccaatt tcagttccct
tcactcattc agactcaaaa 161220gtatagaagc acaagtcact ggtataatct gaaaggattg
caatatggta aatcagttaa 161280tcaaatcata aaaggagccc tgcaaactgc agtggtggtg
aatttggaaa gataaaaagt 161340aagagagagg aacagaaatt cttcccccac atctacccct
tagcgtttca aaaacttcat 161400gccaaaaatg caactggtaa atgtacagtt tctctttcca
aggaccctga gcatgagcaa 161460gctgaaaaca tccactctgg attcaaagag ctcctgacag
ccttcaacaa acccagaaac 161520aactactcgc tgagaagtgc caaccgtatc tatgtggaaa
aaacctacgc attgctgcct 161580gtaagttgaa tggttttatg tcaaagaaga aaaagaaaaa
aaaagaaaag aaaagaaaag 161640aaaaaaagaa aaaaaagttt tgcattgtat tcacttacca
ttaatagaac agatctgaag 161700ctgtccataa atgctgcaaa tgatgagtct tggcttccag
tgataaactt cattggaaaa 161760tacaatttgg tcttctccca gtatataaga atgcacttgg
ctgtaatgca ggactccttt 161820tcatgtaata cagctttatc actaggaacc tcagtacata
caattgaaaa tgagatatta 161880aaatacacat atccagggga tttgcacagt cttccttcct
tctccaaata aaaatgggaa 161940cgagagaata agagtatttc ctttggttat ttcctaacca
ttaactcact gctcaataga 162000gagcaaaatg ctagatcctg caattgcctg tgtgcaaaaa
gttaacaaga agtccagtag 162060ctaacaaatt actttttgga ctataaaaat actgtacaat
acagaatgtt tccttctttc 162120gttctttttg tatgccattt tccagacata tctacagctc
agtaagaaat actataaggc 162180agagccacag aaggttaact ttaagacagc accggaacaa
tccagaaagg aaatcaacac 162240ctgggtggaa aaacaaaccg agagtaagtt gagctcaact
ccaacatcct tcctcttccc 162300actgttccct tcgggaccct gttcccactc ctgtgactgt
ggcatccagg tcatgccctc 162360tggtgtgggc agtagatggc tgtctgcttc cagctgcttg
ccttgagact gtggcgtttt 162420ttcaggcagg agccaattgc tgtcagctag ccaggagaac
tgggcaacaa acagcaaaca 162480gactaactgg tttatgtcag ggaagtaatc cagggagtag
ggcactgagg cttgcacttt 162540ttcactaagg agttgaactg agtggataaa gaatcaacac
attccctcac tgtgttacac 162600tggagtaaag cctgactttt ctgatttcaa aaggtaaaat
aaagaatttg ctgagttcgg 162660atgatgtgaa agccaccact aggttgatct tggtcaatgc
catttacttc aaggcagaat 162720gggaagtgaa atttcaggca gaaaaaacat ctatacaacc
cttccgactg agcaaggtaa 162780gctcctctgg tgtcctcctt aaaacaagca gactggagac
tgcacccact accatctttt 162840atttcatcca tcctttaggc attccttggt aaacagactc
tctgaaaagt tgtttacagc 162900aaaacatgtc agttgtcagc tcaccaacat ttatggaaca
ttaagatgct gctcaggcaa 162960aggataacta gatccagatg gaacacagtt tccaaaaatg
ctagggtcaa ttaaagcctt 163020tttgcaagac tgaggtataa gagctacatt gtaaaaatca
gatattaaga gtccatcctt 163080cctgcacagg aactacatgc tatgctatgg acgagtgcag
tacccgcgcc tctgtgctgc 163140acaatccggc tgtgaataca gctgctaaag tatggatgca
gcagcacagc tccactggat 163200gggtgcatgg ccgagtgaga ctagaagtaa tgttgccaga
gaggagatca caaaaaggct 163260gcacaacatt tatcctctca caccatagct gtttcattgc
tgtaatgttg ggtgcctgta 163320tgccatgaat gctccatccc cctaattctt gaagatattt
ctgactccct tcctctctcc 163380tctgtgggtt gatgtgcatg ttctggggaa aagagaacat
cagttagctc agtccccagc 163440aaaatactct gggaaaagag ccaagatcag caatattgtc
cagtcaagaa aagccttgga 163500aaaagaatgt caaatctctg ttacaaaagc tgcttatgaa
agtttcctct ttacaaggaa 163560ttcctttttt caaggaataa ttttaaccga taaataaata
ccttacagaa caagtccaag 163620cccgtgaaga tgatgtatat gagagataca tttccagttc
ttatcatgga aaaaatgaac 163680ttcaaaatga ttgagcttcc atacgtgaaa cgtgaactca
gtatgttcat cctacttcct 163740gacgacatca aagatggtac tacgggtctt gagcaggtaa
aaagttctgc tacatccatt 163800ctgtatcgcc actcagtcat cagaacaaaa aggacaggct
gatgaccata cggccctttc 163860tttctttggc agttcattcg gcagaagtag cgcacaaaaa
cttgcagcat tatgtctcac 163920atttgctttg cagcctgttc tctggtcatc agtaaaagca
atttatattt catattttca 163980gctgaatgtt aaatacgcca tttaaaaatc tgtttaaatc
attaaaaaaa aaaagacaat 164040cataattaat tggtttatcc ttgcaattat caaattcctc
tcatttctta aacaacagct 164100ggaaagagaa ctcacctacg agaggctgtc agaatgggct
gattcaaaga tgatgacaga 164160aactcttgtg gatctgcacc tgcctaagtt ctcactggag
gacagaattg acctccgtga 164220tactctgaga aacatgggaa tgacaactgc cttcacaacc
aatgctgatt tcagagggat 164280gactgataag aaggatttgg ctatttccaa agtcattcac
cagtcttttg ttgcagttga 164340tgagaaaggc actgaggcag ctgctgctac agctgtaatt
atatcattca caacttcagt 164400tatcaatcat gttctgaaat ttaaggttga tcaccctttc
cacttcttca tcagacataa 164460caaatccaaa acaatcctgt tttttggcag attctgctgc
ccagtagaat aaattattcc 164520tcactcctag agggatccaa agttcacttt tcaaaggaaa
aaatgtgaac tgtagtatta 164580aaagctcagc cttcaatcat atagccataa gtactggaag
tctatgtctt tttccttaag 164640taaggcagca cccagacacc accacgcgcc tcgaagactg
tctctctact gctcctttcc 164700attatgctca tgaaattgcc ttttatagaa agcaaatgct
tgaggtacaa ttgctagcct 164760ctgttcacct tgcgttttgt ccttatttct ctaaactctc
aagactgagg ttgataagta 164820tcccaaccag caaaaaagac caagaaaact acaacaatgt
gccttattgc tacctcttac 164880tgaaatgtga cctaaacaat tcaaatctgc ttcccgtttt
cattaacata attatatgtt 164940tcctggctaa catctgcacg gtctccttgc tacctggatc
attgataagt gtatgatttg 165000taacttacga gtgcctttca gctaagatag tcccggtatt
gacagaaaca ccagtaacat 165060ttttatggat gcttcacttc attattttgc catgatctac
atttaaacaa taaatgaatt 165120tggaactgtg tttatgctat gcaagattct gactcacgta
gctcttttac agcatcctgt 165180ataatgggtg gctgacacat atttccattc ttgttatttc
aaaccaacca tcacatcacc 165240gctaacgaca aagtgctgag gcactctaat aaaccagggt
cttactccca ctagatttca 165300tacaacactg aaaacactgt cgttcaacgt gttatcgtag
acatatacta gacacaccaa 165360ttcaaatcaa agcctgtgat aacagagtta aggcatttgc
ccagtcttgt tcaacagctt 165420caccaatagt ctagataaag ggatagagtg aatcctcaac
aagtttgctg acaatatgaa 165480gctgggagga gtggttgata caccagaagg ctgtgctgcc
actcactgag acccagacag 165540gttgtagagt tgggtggata gaaacccaat gagcttctac
aagaacaagc atagggtgct 165600gcagctgggg aggaataact gcatgcatca ctacagatca
gaggctagcc tgctggaaaa 165660aaacctctga agagaataag ctgggtgtct tggtagacaa
cagattggcc atgagccaac 165720agtgtgccca tgtggcccag gtggccaatg gtatcctggg
gtgaattaat aagagcgtgg 165780ccagcaagtt gagagaggtg attctcccca tctactctgc
actggtgagg ccacatctgg 165840agtactatgt ccagttctgg gctcctcagt taaaggaaga
cagggaactg ctgaggagag 165900tccagtggag gactgccaag ttgattaggg acctggagca
tctcccatac aaagaaaggt 165960tgagagaccg cagactattc agctcagaga agaaaagact
gaggggtgga tctcatcatt 166020gtttataaat atctaaagta caggagtcaa atgaatggac
ccagactgtt ttcagtggtg 166080tgcagcacaa gggggcaatg gctaaaaact gcagtgcaga
acgttctata caaacatgag 166140gaagtacttc tttattttga gagtgacaga gcactggaac
aggcagccca gagaggttgt 166200ggagtctcct tctctagaga tatttaagat ctgcctggat
gctttcctgt gcaacctgcc 166260gtagggaact gctttagcag gggttggact ggatgatctc
caagaggtcc cttccaactt 166320ctatgattct gtgatctgaa cttgctttca ctgtaagcat
tcagtttccc attgttgacc 166380actgcttatt gcactatcag cgccccatgg acctactgag
aaagctcatt ttcttctgca 166440ttatgataca caagaaataa aagtctgacc agagcaaata
gcccagaaga gtggcttcca 166500aacatccaat gctgtatcag caacatccat ttcttccatt
agcttaggag ggcagtagct 166560gtgctatctt atgcctataa ggaattggat aaagttttgt
agccattatg atgcccacct 166620accacacgtc acaggacatt atttcaagca acaagatctg
aatgctggag ggaagtactt 166680tagaaatata tactcctagc aaaattccac ctgtaactac
agctcactct atgcctcaac 166740aaaaggtaga ctatttggaa atatatctcc acccaacaaa
gtatatatac gccgccttct 166800ctgtacactg tgctgcttct aaactcagtg ccctccctca
cctgtaaccc ccagctcagg 166860gttcagctgt ctgtcactga ggacccactt tggttcagcc
cagctgcatg ctactgactc 166920agctttgcaa agaattgcac attgtaaatt gtataagtgt
ggaaaagcac acaagtgaaa 166980actacacaat ataagtaaag taaaaacaca aagaagttac
tgtcaggatg gaatagtcag 167040catagcaagg actcgagagg attgcacgta ggggtgtcaa
acttcaatta aaaaaaagtt 167100aagaattgga attctccagc ttgttcaggt ccttctgaat
ggcagcaaaa ccctctggtg 167160tatcatccac ccctccaaat tttgtattgt ctgcaaattt
ggtgaaggtg aactctatct 167220cagcatcctg gtcattaatg aatgtgtaaa tagtactgac
actggtgtta accgctggag 167280tacaccacta gtgacttcac cttcagctgg acttcatacc
actgatcaca gctctctggg 167340cctggctggg ctcacttatc taacccatcg tttgtcagcc
tggccataag gatatcacag 167400gaaaaagtgt taaatgctgt ttctaaagtt aaggcaaaca
acatttagta ttctcccctc 167460atcaaagaga ctagctgcct catggtagaa ggcaatcagg
ctggctaagc atgacttccc 167520cttcatatat caatcctgac tactctcaat ctccttctta
ctcttcatga gtttcacagt 167580agtgtccaac tctgtggtag cctgacctcg cagcaagcag
aaatggcaga gagagtacag 167640aataataaag acaaggaatg ttacagtgct ggtgtatttt
tagtactcaa ggcttggatg 167700cagaaaaaaa ccagcaacaa aacaacctat tttagaacta
aaatgcaaat gtaccaatat 167760tttttaaggt ttttatttca ccatcagtaa ctggaacaag
aaaatccctt ttaaaatatt 167820atttatactc cagtcactta caggtctatt gcaaccactg
tgatacttat tctcctttcc 167880tctcttgtag gacaaggctg aatgggactt cccacggagt
aaaaaactgt aactggactt 167940aatctagcag tgattttaaa ttgtgctgct tgttaaccag
gcataaatgc ctagatgtac 168000caaactatag agtaaactga tcacaggtat aggagtacaa
cagcagaata gaaagataca 168060gcctcacatc cacactgaga aaggtgagga tgaaagatga
attcatgaga aagcccgaga 168120aaagtgactt gctttatcag aaaaatttta tgaccatttg
tcagagagca acctatttgt 168180ggccagcagg ggttttgatg ctgcaacaca ttgtttctac
cattctgttt gcagtaagcc 168240actctttcct cttctccacg tggcacagca aaaccaagag
caccttttct ttcccatgtt 168300ttctcccctt tgaaactcta ctccctctta ctaaacagga
tcttgcagca gataccatag 168360agtgtccaca cagtttgggc acccttttgt aattttcctg
tatttttgcc caatctttgt 168420agtttgtagt acagtaatta ttgctattaa ctgctttctt
acctctccta aatattcagc 168480ccaacaggta acagcagttt cagatatgta gcaggaacat
ttcttaacca cataactaaa 168540cataatagca ttcgatagat ttctgaagaa agaaaatgag
attgatattc caggtaggaa 168600aaatgtgcac acttagagag agagcatgtg aaaaaagata
ggtatacatg tcactcctga 168660aaccttgtcc tgtgtatgta tcagtcacat tcaaagtgaa
tagcaccata aaaatttagt 168720tctagttcta gatatggttt tgtactcctc atacaaatgc
ttttgcacat aggttgactg 168780catgctgata actggtttac tttccagtgt cataactgtg
agcagagggc actcctaagg 168840caattctgag taaccaaaca tgtactagaa aatatataca
atgattgcaa aggaaactga 168900tcaatgcatt tcatccaagc cacgcagata aaaactggaa
ttgctaatta cattggttaa 168960aataaaatgc ctgtgtacag ataactgtct tgcattatat
ctttactgat ctgattccat 169020ataacatgct acttcgactc tctactacaa aacaacaagc
agtttcagta aatcaaataa 169080ttacaagcag aacttccaag cagcttggca gcttttcgtg
agtggcccaa actccctccc 169140ttcatagaag ataataagct tggcacagtg cagggcctgt
aggttatcta aagcatcttc 169200acctcctctg ctaggaggaa atgcattctc ttaaagcacc
acccaccagt tcgaataagt 169260accatgctca cgtagacttc atcacttgct ttaggtgact
gtgggaatac agtctgagtc 169320tccttgagga tctgactgct gagacataaa aggattaagg
gtaaaatatc acaacataag 169380ggctttgtgt gacctgacaa ccacaagtat tcaaatcttt
ccactctgaa atttgacagc 169440aggatgaatg acactaacag cctacttctg tactcctttt
cagctaaatc tttgagatta 169500gtttctctta attccctagt tcatagagta aaaagaaaag
gaaactgtaa aattatttca 169560acgtgtccct tgtcaatgca ctgcaactct attcttttct
gcaattccta catacttgca 169620gacaaagatt gaggagggca ccactgggaa aaaggcatac
caggcttcat gtttcaggac 169680acacaccaac taacagtggt tttgaattgg cctctctacc
ctcatctctc taattctgaa 169740agccagtgac ggagagaaga aatgggaata aatgagggaa
caggcatatt aatacaagaa 169800gaggatgttg ggaagagaag ggaggtgcaa ccatttctac
atttccaaaa gtattcgttg 169860cctcacagac cgccacagat cctgactgaa gaaacatctt
aactcttccc tctctcacag 169920gatttcaggt agtgtaaata ttcttatcta aataaccagc
tttattttga cagtcttaaa 169980aattcatata aattttccag aatggagagg gtaaatggta
acttaaatgt catgcaggga 170040aatggagcac tcaagtcttc atacaaactg cagcctttag
gaaaacactg cacaaagggc 170100acagtgttca ggatgcacat ccagactggg caaaacctgt
gcagcacata gagagcacaa 170160gagtttcctc acttgcaggc agggtgccct aatcaccagg
ctaccagctg atttaggatc 170220agaggactga acacagaacg cagatgataa tgcactccta
ctgatcctgg agttgaacac 170280acgtgactgc atgactccat aacacagtgt tttagacaat
cagcactgtc caagccactg 170340ctttattcgt gttaaataac tactggatca gcacccagcc
ttttcctttc ctgaatgacc 170400aaagtggcta ttagacaggc agtgctgata caaacacaca
tgctctctca agccctagaa 170460atgcttgatt gctgtgtgca aattcagttt tgacacgctc
tggcaatagt ggagatacta 170520gtttgaaatt ctttaagcaa aaacattcag ctgagcccag
ttctctagtt atgctctggg 170580agtatctggc tgatcagctc ctccaggcac agcagtaaga
aataccaggc ttaaggtaaa 170640atgcttaagg tatgagctag gctgcagggc attcacctct
gaagcacacc tgagaatgat 170700ccaaagctgt tttggagcac tgggaagctt tgatatccaa
atttaagaat cccaaaagct 170760ccagctatct ctaagactga acagcagctg gttttgagga
ctgctgcttt tgatttcagc 170820atgcaaattg tgagtgaagc tggactgaaa attccactca
ttctcattga cctaaggcaa 170880gtctttcact tgatgcaatc ctgcaccgac aatggaaatt
tctgcattta tcctaatatt 170940tgtcaacagt actaaattca tctaaaatat gttagttaga
accacgaacc tattctggat 171000ccagctatga aaaattactt taaccctggg gtttctatgt
ttttgttatt atctttgatt 171060ttgtcccata aagagggaag cagaaagaga atgggaaatc
tgcaggttct gataaaccag 171120cacgaaagta gaacagagct gttgcttaaa atgggaactg
tttggagttg tatgtaagac 171180acaagagctt acttgaaaaa cagtcattaa caaatccagc
tacagttgac aaatgcaggc 171240ctacgctcac aaagatcaaa taacaatcta cagatctcca
tcagccttct aaagcatgtg 171300gcaaatggca tgaattcgca atagttcttt cagcagaaaa
taatgcagtt taaggaagta 171360ggaataaaag ctcagcgcag cacagtggga aatatttcta
tttcgctccc tcagaatgga 171420aggataattc taagaaactt aagtaaatgc tttttaagaa
caagtttggt ttgggaaaac 171480gttcctaaaa tagtgtttgt ctgatgatga ataagcgaaa
cggctgaaac aagaatataa 171540gcatttaaca gtgatgggaa agggagaaat agcgtaggtc
ttaaaaaggg acttgctatc 171600tgactgtacg tggcgttttg gccattacag agccatccca
tcagcgtgtg tacaagggtg 171660caggcaatct cctaccagca acttcccatt ttgttagatg
ttgtgaagat gcagtcatca 171720cctccctctg ctaatcattc gtctcaggct gtattgatgg
agtgtctttc agcatcaacc 171780aacagcttca ccctggacct ttacaaaaag ctggatgtaa
cttccaaagg acaaaacatt 171840ttctttgctc cttggagtat tgcaactgct ctcgctatgg
tctatctggg tgcaaaaggt 171900gacacagcaa cccagatggc taaggtaagt tctgaactta
gcagtgtatc acttaaagct 171960ggctcccacg tgacttgcat tattcacctg cctacgtgga
acaaaaagga gcaacatgag 172020agaacagtga tccgccatca tgttgggtgc aagaaaaaac
actgcatgca gtcctgcagg 172080acactgttgt tccaacttat ttccaagagc ccccttctct
gctaccacct ctacctttag 172140cttaaaattg ttctggcaga gtgaagctat ggacattaga
gactgtcttc tcattctcca 172200cacccagtta gtaagtcctc tgaacccaag cttggctgca
cagcagcagc agcaacatct 172260ttgcttgtga catggttagt ccataaataa ggtaatacac
ccatgaagaa gggaaggttt 172320agattggatg tcaggcggaa gtttttcaca gagaatggtg
aggtgctgga acaggctgcc 172380cacagaggct gtggatgccc cttccctgga ggtgttcaag
gccaggttgg atgtggccct 172440gggcagcccg gtctagtatt aaatgtggag gttggtggcc
ctgcctatgg aaagggattg 172500gagcttcatg atccttgggg tcccttccaa cccaagccac
tctacgattc tatgaataat 172560ggggatatac tagaaatgaa aatatgatat catttataac
cacttttgca aaactttcgg 172620tgatgtactc ctagattata tgcatttaca taaatgcatt
tatctgtgta atgtactgta 172680gagttgtaca ttggtgcctc aatagtaaga ctacaaacca
tcctatgttg tttgttctgc 172740ctgataacaa tctgaaaata aattccacat tgctaagcat
gaattgacca tttctccaaa 172800tcaatccatg tctgagcaat cacattgatc tgttattaag
tagtaaatga ctaaaattaa 172860tataactatg atacggttat agaatctaaa tctagaccga
ggtcttgttc tctataactt 172920taatagacta acatttgtac gatggctaaa ttatcctaag
tagaaaacta acatcattac 172980gtaacactag agcacttcta tcttcacaaa acaaactgtc
cttaagaaaa tttatcactg 173040ccacaggttc ttcattttaa ccagactgca agagaagaaa
gtccttctga gatgacagca 173100ccttctctgc ggagcccaaa gagaagagac atggtattta
ttttgacaag gctcacagaa 173160acaaatctcg gagaatggga cagcacaact gccttaaccc
tctgaaattt gtctgtctat 173220acctactgtc ctattgcagt aagaactata cataaaaaat
gtgaacaagc aggtaatatt 173280actgtaaaac tcacgaactc agaacttcaa agcagaacag
agacaccaga agcattctga 173340ttgtctttta cactttgtta cttgctttta gctgctctat
ggcaatagga atacctaata 173400ttttactaac ttcaggattt tttttttttt ctgggctgta
aaacagccat tgccttcaca 173460tctgactcca gtcctgctct cttaaactct gttccacctt
tgctatgcat gcacacatca 173520ggcaaacaaa attcccctgt aagctaacac acactgattc
acccttgctc atcagctccc 173580cagagacttg acagcaggag gaactgcaca gaactcctta
cgcctcaaag gtctgtcaac 173640tcaaacaagc tgcaattctt tagcacagaa actaagaaag
ctcaaagaaa ccaagttaga 173700aaaactggaa ataaaaagga gcagttacat cattctccat
ctagatggcc gtggattaat 173760atttaagata aatcattatg tccacattga ttttagctat
tcactgcctt tctaagttgc 173820acagcaaggt ctgcctgatg tagccttcca gacatttctc
tccattacct cttcactatg 173880tacccattgc cttgcaaaac ctatctacta tcctgttctc
taccaagttc ttccccacat 173940gtcctttctg aaagcctgta cctctgcctg tgtgaaaaaa
taccagagga aggaatgcct 174000cctcaccaat cttatgacaa gcccccatgc atcagcagca
aagaatctcg gtgtctcaca 174060tgtagcgcat ggtacatgcc atggagcagg aaattatact
gaagcagctt atccctacac 174120tacgaaagca acagctgaca agcaagctcc tgctccctaa
aaccatcacc aaggacattc 174180tggacaggtt ccttccaaca tccaagcagt aacagcaaaa
ttcacatcaa atagaaaatt 174240cgaatcaact caattacatg acatcagtat ctgtctgaac
agagtagctc ctcaaaagct 174300gcaatgttgc cttaatgatt tttgtgataa tcaaaattag
gcttgactgt gactggaatg 174360agatgaccca atatcctggg tgcaccatct gaggacagct
gatgtcctca aaggtgtaag 174420cagctccgca gaatcaaatc ataaccccac agacataaaa
caatgtatcc agttacacac 174480agtgctgcaa aagtaccaga tacccgaaga agagctccat
ccctgcacac actttttaaa 174540ttaaaatgac ctggggattt taaataacca tagaaagtgt
aatgcttcag ccaaaaatat 174600ttcaatacta attggtgcct ctttccaagg gtcttgagta
tgaggaaact gaaaacatcc 174660actccggctt caaagaactc ctgtctgcca tcagcaaacc
tagaaacact tacttgctga 174720aaagtgctaa ccaactgttt gaggacaaaa cctacccatt
actgcctgtg agttgaacgt 174780ttctgcttaa gaatgttttg acaaagagta tttgacgtac
atatttgaca atcataattt 174840tccaaatgtt ctgcctttta aatctactgc agcctttaaa
actgtaagag ttctacagtt 174900gaactacaga aagctcctgt ctcttttgag cctatttctt
gttagcatca ctgttggttc 174960acttctcctc taaaggaagc ttctccatat cagtggtcat
cttcctcccc cttctcacta 175020tctattacat tctagcattt ctgagattgg aggccagagc
tatatagaaa agtcacacca 175080tacctttaca gagcggcata ttaacgttct cccttttatt
acactgtcat ttccttcaac 175140ttttcacatc atatttgctt gtttgactat tactgggcag
attatttttt tttttcatgc 175200aactgtccat tagaacttca atacctcttt tctaaagcaa
taatcatcct tattagtatg 175260ctttgctgga tcattttcag atgtttcaat attgatactg
taatctattt ccttcttctc 175320ttagtttcta gccttttaaa caagatcttt ctctgtagtc
ccacggatac tcagcttctt 175380caaaagcctt tggtgagaag tcttccaaaa tgccttttgg
caatcctaat tggctgtagt 175440ggtttgacct tctcatttgc aagcttcttt acaccttcaa
aaacctccaa aagattcatg 175500atgtaggact tattgttaca aaatgctgtt ccaacacatc
atgcttacct acgtgcccag 175560taggcctgct tatccatttg ccctgtacag actcatgagc
ttgtaattct gtgtatcttt 175620gaaatctttt taagaaatgg cactgtatta atcatcctcc
agtttcttgg aaggtgctgt 175680taaatgaggt tagcgttctt tcatcttgga gttaagtttc
agtctcctga gtaatttctc 175740agtcattaaa ttcaatcagc cttactgaat gcttgttgct
catgttctgc ataattttgt 175800gtaaccgtgg tttaagacta atcagggaat ttcctgccgg
gaatctccat gaacagtttc 175860atagaagaca gtaatgtaaa agtcagttct agtttattct
accataacat tcccttcata 175920tctgaccctc tactgacctc acaaactctt ggcagcttga
ctctgaagca aatcttaaaa 175980cattgtttca actgtccttg aagctattct ttcttcttgt
actgaactct gtcataaaac 176040ataaaaatgt acttcaagtc agtctgagaa atcaaaaata
atttaaaaaa atgtgtagaa 176100tgtttatctc ataggatttc aaaattacaa atttgcatgt
tggatttaaa acacaaaact 176160ttcaagcatc attttttgtg aaacacaaat actgaatttt
tgatcagtcc ttgcttatta 176220tttaacacag aatcataatc acagaatcat agaatggcct
gggttgaaaa ggaccacaat 176280gatcatcgag tttcaacccc tctgctatat gcagggtcaa
caaccagcag accaggctgc 176340ccagagccac atccagcctg gcctggaatg cctccaggga
tgggcatcca caacctcctt 176400gggcaacctg ttccagtgtg tcaccaccct ctgtgtgaaa
aacttcctcc taatatctaa 176460cctaaacctc ccctgtctca gtttaaaaac cattccccct
tgtcctatca ctgtccaccc 176520tcataaacag tcattccccc tccagtttat acactccttt
caaatattgg aaggccacaa 176580tgaggtctcc ctggagcttt ctcttctcca agctaaacaa
gtccagtccc ctcaaccctt 176640cttcatagga gaggtgctcc agccctctga tcatcttagc
agccatcatc acagaatgta 176700tcagaatttg ttttacgggt tatctagttt caaatgaatt
acattttctt ccagtatgta 176760ttagtatgta ttgcatgatc tgttgagatg atctttttct
actatttttg tgcttaaatt 176820taactatata agcatacatt ttccaattct atccttcaga
aatttttaca actgatcaca 176880aggtactacc aagcaaagcc acaagctgta aactttaaga
cagatgcgga acaagccaga 176940gcacagatca attcctgggt tgaaaacgaa actgagagta
agtatcgctc tgatggcttt 177000ttcttttctc acttcaaaat catttgcatt tccacttgaa
ttgctcttgc agtaagggat 177060ccataaagga tggaaactgt ggggaaatga tgaacaaatt
gcagttaaat gtcttgaaga 177120aagccaacca ccaaaactaa ctgctgcccc ttgcaaagtt
tttcccttga tttttcatgt 177180catagtctct tctgaagtat ttctgttcat aaggaagcag
agtggatact acatggctcc 177240actctgatca gtgaaggttt tacttctgca agcttcaact
ggttgcagcc aactccagag 177300aacttccacg ctttacacac ttcttaacat cttttactac
taaaactgaa ataaatatgg 177360tttaaaaaac agtgatgctt caaaagccat ttatgtatgt
acgctgtgaa aaatgcacag 177420ggaaaaaaaa tctctgagtg taaacacttt tgttagatag
ctaggcatag agaaagcaca 177480tctgaaattg gtgagttgtg cattcgcagc gaattaacag
tcctatctat ttgattttta 177540agggaagatc cagaatctgc tacctgcagg atctcttgat
tctgacactg tattggtctt 177600agtaaatgct atttacttca aaggaaactg ggaaaagagg
tttctggaaa aagacacatc 177660cgagatgccc ttcagattaa gcaaggtaaa ttccttcaaa
atgtctatta tggcagagca 177720agaatcctct aaatatttca cctgcatttc acatcccagt
acaacactac ttacagcagt 177780agcagatggt ataaactctg agaacagcaa cagtgaaaat
aaatcagcag tctcatttat 177840acagatgcat gagattagga ttttcagtta agttagtagc
ttcttggcac caaaacagtt 177900gaaaacacca tggttaagca gcttaaggac aagagaaagt
ttctctaagt actgagatat 177960cattttcaga aggaattgag ctaattctga gagcagtact
tcgacaccta ggtctctttt 178020catgcttttc agacagaggc tgtataatgt gagctcaagt
agcctaagtg ttctttccta 178080atgccctggc cattgcgtaa aacctcacgt ggaattctca
agagggtttg tcattttagc 178140cagatgcgta tggatgatgt gttcagcatg cattgtgggc
acgactgagc ttacagtatc 178200tcagtgattg tgcatggaca atttacagta gctgacagca
tgcatacttt cggcttgtgt 178260caaaggtgag caaaaagaat tttcattcag aacacgttgt
ttgacatgag attacgagtg 178320caaacacctt ttgtatgtct ggtgatgtga agcaattgtg
tcgatactgt ggctgtgtta 178380tctgaaacct actacattgc atgcgcagtt ttaggacctg
taatagtaca cggtgcacag 178440aaagggtttc attcacagag tggctgatag caaagcctgc
aaacagataa gcttttgcac 178500ttgtgtaaca atggaaaaga gagagtggat atatcagtga
aggtctctga gcataataca 178560gcgtaagagt tcagatgatt actgtctaac gcgatttcag
ttggtaatcc aaacctctac 178620agtttgggaa agagaaaaaa caagcagagg tcacagcaaa
tatggtccat aggtaaattc 178680aatcaatcag tgctgtccgg aagcatacaa aagagttgat
gacatccaga gaatgaaagt 178740cagcattttt ttcccctccc aatcaacaca ttcactcaag
aaatgtaaag ttttggggaa 178800aacttgaaac atacaaagta gtttcttgtt taccaaagct
aactctttca aaagagtgag 178860aaatacattg catgtaatta tgttatcagg tggtgtctgt
gctttttttt tctttttttt 178920cttttcttct gaagatccct ttgactttga aacaggagaa
atggcactgg gaaagaataa 178980tgccaagtct tatacttgtt tacaattttt ttttgccttc
agttcaacaa agcaagtaat 179040ctttaccatt caccttaagg aataagtaca actaatcttt
ttcttttctg ttctttttta 179100acatctgaat catttcacag accaagacta aagcagtaca
gatgatgttt ctcagagata 179160catttttgat gctccatgaa caaacaatga aattcaaaat
tattgagctg ccgtacgcgg 179220aaaatgaact cagcatgttc gtactcctac cagatgacat
cagtgataac actactggtc 179280tggagctggt aaaactgaca tactgcatca cacgcactac
aaagcactaa cagaaataga 179340tgaaaacagt gaggaagaat gaacttcaaa tgacacaatg
actgctcagc ctaggtttca 179400gggcatctat taatgatgca aaaatacaaa tctacctgag
gatacaccta aaaaagtatg 179460cccactctac tctcttagcc tattcggtgc ctccttttct
acctccagaa tagcagaata 179520acgaaagcaa gaatcaaatc taaaccactg tgccccagaa
ttaatcttct gagggcaaca 179580ctaaccagtt ttatgtcatc cgcagtccag atttccacct
gatactttgt aacgaggctt 179640ttcaaactcg gggctgactt accttgaccc atgaggtatc
agcagccact catgaccgtg 179700ccaggattag ttcctgaatc taaatacatc agagcttcag
aatctaaata catcagggca 179760aatatctttt tatttgctct tgaggtccca tgcattccac
ttacttacca ctactaagag 179820aaatgcctta caaattcaca cataccaagc acttattaat
gtggttaagt tggacactgc 179880ataaaagcaa cacttctcat atccacctcc aaaataatga
attattctga aggttcactc 179940tacacctcac tgcatttaag gaaacagata gaagtacagg
tcactcagca ctatgcagga 180000tcacatccta agaatatgca gcacatttca gctgtactca
cagctggtag ttggaccttt 180060taaatctaga gcattagaca ccaatgtatg catgccttct
tttttctgtt gcattatgac 180120tatattctta taaaattcat tgcaggtaga aagagagctg
acccacgaaa aattagctga 180180atggtccaac tcagcccgta tgatgaaagt cgaagtggaa
ctgtacctgc ccaagttgaa 180240gattgaagaa aattatgatc ttacatccac tttgagcaac
atggggatac aaaatgcttt 180300tgaccctgtt caggctgatt tcacaaggat gtcagcaaag
aaggacttct tcctatcaaa 180360agttattcac aaagcttttg tggaggtcaa tgaagaaggt
accgaggcag cagctgccac 180420aggtgtcctg gtgttgaggt caagaacacc tagagtaact
ttcaaagccg accacccttt 180480tctcttcttc atcagacaca acaaatccaa aaccatcctc
ttctttggca gactatgctc 180540accttagtca gagtcactcc ctgctctaca gagcaggaga
tgctggcttg ccagctcaag 180600ggcagagctt gatactcctg ctgcagctga gggactaaga
cctgcactct ttcagactac 180660acattccaca gcccaaggca aagcttcaac tactccagat
agccatagca gtgcctgtag 180720atgcatttga ttccttcctc ttgcagcagt agatacaaac
acatggcact atcttcgttc 180780tcacaagtag agcacctgat tcaggtgtgc atcttcaccc
ttccaccctg ccataattag 180840cccctgctcc tctgtagctc ttgactagtt ctttttgtta
cagaggcaca cacagcccaa 180900gcttaagtct ttaccagttc acttccattc tactgattgc
ctgaaagaca taacaagcac 180960acactcccac gtgggctatt tcctcgcacg gagttacagg
tgtgacagaa gagcctgacc 181020catgctgctg actttataca aagcagcacc tgcttcaaaa
atagcagtac tgataataaa 181080caacccctcg tagcttgatg gtgctttctg tcagctctac
caggagggga aggcagaagg 181140ggaaatcaag cagcgacaag aggctcgcgg aggtagcgac
ctccgagcta aaatggccgc 181200ctcccactgc tgcagcgagt gctcagggcc gctttccgca
gctgagctcc agccctctcc 181260cccacgatgg gcggcccgtg gctaggcaaa aacttccggg
aggagggcgg ggcagaggcc 181320aggggaaagc tggtgctcgg ctgggtgagt gtggagggtc
tgtgttgttg ttttctgcgg 181380gaaacacgca ttggtttttt gaggggagac ggtagcgttt
ccctcgcggc ggcgctctga 181440gcggtttcgg cgggcgcggc cgccgggcgt tgaccgggtg
ctggaggcgg gaggggcccc 181500gcagagttcc gcaccgctgg aatccatccc tgtcatccag
ccctgcctct gtgggttttg 181560tggcaaacag gcggaaatcg atggagaggt gcgagcttca
gcctgttctg agtcacaggg 181620agagagcttg gccaattgtc ctgcgcccag ccttattgga
gctgtaaggt gcacgggatt 181680aaatcgctcc tgcttcaggc agaatggaag gactgtttca
gtccaagttt tcttttcatc 181740agtgttttta tggctatggg cagaaggaaa catgagtaca
gctgcagctg ttgaacgtag 181800ccaagctcct accaagaatt tgtcttagag gaaacatgcc
tgaggaaact tgctgctacc 181860gcttgtttga gatgatgaat cattaataca aagtaggcgt
tggctctgta ttttctagca 181920acgtaccaac accaggcact gccttagggg aaaaaaaaca
aaccaccttt actactagtt 181980gatatcctgc gatgtctgct ggcacttatc tgtaacttac
tccacgttct ggcactcgtt 182040gctccttcct gtaggtatgt agtataactt cggattagtt
agctacctgc tcggctgacg 182100tatgtgaagt ctgacaagca ctgagctacg tatgtgccat
gaagttccca ataaaccgtt 182160tactttattg cgtctgtttc catcgtgtag acaataaaag
gcaaactgca gtggactttg 182220attttgtacc acagcaggaa accccagtaa tctgtaatgc
tgaccagata aatttcgttt 182280gaatattgta gatcgagtca ttcagttgga ttctggcaga
ctgactgcta ggtctagaac 182340acaagtgaag taatcttgaa gggaatactg aagacacaca
gactttgaga aggtgagttt 182400ataattctgc cattctgata cctttctgct ttggttttcc
tgtaaagcaa ataactgtct 182460ctgtggagcc aaaggagact tattctacca agtcctagta
tgctcatctc aaaaaatata 182520gtattattta ctccatgaag aagaccaatg acttttcctc
actacaagaa agacattgag 182580gtcttggagt gtttccagag aagggcaaga aagccgtgaa
gggtctggag cacaagtctt 182640actattgagt ggctgagggg gctgggattg tacagcctga
agaagaggag gctcagagga 182700gactttatca ctctgtacag tgacctgaaa ggaggttgta
gttggcctct tttcctgggt 182760aacagcaata ggatgagagg ggatggtctc aagttgtgtc
aaaggaggtt cagatgggat 182820attatgaaca atttattttc cgagtggtga ggcactggca
cagactgccc aggaaggtgg 182880tggagtcacg tctctggagg catttaagaa acatgtaaat
atggcactga gggatgtggg 182940ttagtggaca tggtgggaat gggttgacag ttgtactaga
tgatcttata tttgctttat 183000ggtttatatt gagaaatgta aaagacagaa ataggttgtc
agtttgtgat caaataaatt 183060taagccaatc ttcatttttt tttttctcct aggctttgaa
ccatggatag cctcagtgca 183120gcaaattcca cttttgctct tgacctttta aatgagctgc
gtgagaaaag cagcacaaag 183180aatctattct tttctccttt tagtatttct tctgctttgt
ctatgatttt actgggttca 183240aaaggggaca ctgaagccca gatagcaaag gtatgtatcc
aaacgtaatg tattggattt 183300gatgcatata tcatctactt aatgatatat gaactacaga
tctgagatct gtattacagt 183360ctgtgacctc taattgctga attgttacag tcattctggc
ctcagaggtc agaagtcttc 183420cttaggtatg tacataagca gaacctattt ctattgagtt
tatgtatagg acttactgca 183480gtgtgaaatt aagagattcc tgttttttgg ggtgtgtgtg
ggtttttgtt tgtgatacgg 183540agatcttcct tttatatgtc attaacaggc acctggaatt
tctttttttt tttacttaca 183600tatttgtata tttagagcta tagatgaatc tccagttaca
taaaataatt tactctgtaa 183660tctttttggg cttaatatca gactttgcat acttcaaaaa
tgtagccaga taatcaaggg 183720aaaaaaaatc caacatacaa gcatgtcatg ttaaacagtc
ccagatttta ggaaacaaac 183780aaaaaaatga tcagttgctt gttcagtgta atagcttttg
ttttcacaac ctgtaatctc 183840aatcctggaa catccagaag aaagaagtga tacagggcta
agaacatagc tctgaagttc 183900cagagaatac cccagcaaag attcaatggg gcaaagctgc
gtggccagtg aagagtaaaa 183960ttcataatgt aaacttgcaa ttaaattacc aggagagcag
ttaaggagtg cagtggtggg 184020cctgttgtgt gacagtaggg tcaaatctat cattaactgc
agtgcagttt attctacgtt 184080cactaaggtg cgtgcctgcc tctctctttc tggtattgta
atttggagta gatcatcaat 184140actttttcat ttgtagctat ggtagtagtg atgaggctga
atgaggatga agctgatgtg 184200ttgttttaat gggaatttaa atatttgctt gtgttgacat
cggctccagc agcctatttc 184260ctgttatcgc ttgaaggatc gggtttgcat ctaaggtatt
aaataagatg ctttggtgct 184320attataatca gtgtgaaaaa ttatggaaag ttgttttttt
ttatttaatc ttcaggctcc 184380tttgtttctg gattttaaca gttttgctag gttttatagg
gtggagatta taaatcctca 184440gttctctaag aagtactgtg tacagcatta agaaaagggc
agaatgtgtc tgcactcaga 184500cttctttgga ggctggatgg gttccttaga aagcagggag
ataaaccagg taacctccat 184560agcttccttc caacctcaac cattgtgtga tcctctaatg
cttggacaaa atgaagataa 184620ataccactca cttttcagca acgtaatttc ttgcttatac
aacatctgtg tggatacatt 184680gtacgtgact tgtgtaatga aaaatctgct ggcttcaagt
ctcaaaactc atttaaaaac 184740agaacaattg tgctgatgca agtgtgtcag agattacgtg
gactccacag aaggtatttg 184800tctctctgca ggtgctttct ttgaacaaag ctgaggatgc
tcacaatggg tatcagtcgc 184860ttctctctga aattaacaac cctgacacca aatacatcct
cagaactgct aaccgacttt 184920atggagaaaa gacatttgag tttctctcag taagtaaaca
ttaaatttgg gtgttgtgaa 184980gtataatgta cttgctagct attccccttg aaggttagat
aaaggctttg ggttttactc 185040tccaaatttt tctaggctga gacttacaac ctgagagtct
atgcaaaaag caggatgtga 185100acagaatgga gaagctactt ttagattata tgaatgcaca
actggtgcaa gaccatgaaa 185160aaaaactaaa tcttctaggt ttcttggtcc acttttggtg
ggttctagga tcaaatgaat 185220gacaaatctc cttgcctttg ataacctgta gctatgatga
aaacaactgt tactgctgtc 185280cagcatgggc agaacttttc ttttttctta attaaacaat
ccagagaaca tgctgagagg 185340agtatgtgac tcttaatatt ttccttataa gtatatatac
acaagagggc acaggtacgt 185400tgcatataca ttacatatac attataacat tgtatgttct
ctcactcaag caaaaagaac 185460aaacggaaga aacaaaaaga aacaacccag acaatcattt
ctcagttgag tactgtagaa 185520tgttctggtg tattaaagaa gacatttgac ttcttaataa
caaagaggaa gataattcct 185580agctcagatg gctaataaaa caactgataa gaacatgtca
gacaaaacct gaatggcttt 185640atatcaagct gggggaagag aggatataga tttttctcag
tgtacttaaa aacatctgtg 185700gctgaatgtc agtaaaatgc attgctaaaa agctgtttta
aatgttcatg gcagtcattt 185760atagaatcga gtcagaaatt ctaccatgct gggctagaac
agactgactt caaaaatgct 185820tcagaggatt ccagaaagca aataaatggc tgggtggaag
agaagactga aggtgagtgt 185880tctgcagaac tccctgctgt atgtaatgtc agccaggact
tgcataaaca gctctgtcaa 185940ggtgtaatac tgtcattttt aaagcaaaca caaacctcag
ccattgtgct ctgtctctgg 186000ttggggcata attcccatat ctgatctatc gttaatacat
attagatact ctgtattgca 186060acagttgctt acgtaccact gttcaatttg tgttttctaa
aggtaaaatt caaaaattgt 186120tggcagaggg aattattaac tcaatgacca aacttgtgtt
ggtgaatgcc atctacttca 186180aaggcaactg ggaagagaag tttgacaaag agcgcacaaa
agaaatgcca tttaaaatta 186240acaaggtacg ctacgttaat atgctgacaa tacaaaggtc
tttgtaatac agaagacaaa 186300aattgttcaa gcagatttac ctaaggtagt ctgcatggag
ctccctatgc cctgtcccct 186360tagtatgaac actctctttg tttagtttct gttaagtttc
acataattac taaaaacttt 186420aatatcacat atttatttta tactctctct tttttttcct
ttactctttt tgtttgtgtt 186480tcagttggtg aacttgacta tgtcagtgta aaatctgcat
gggcaaaaaa cattcatagg 186540ttccaggcag aaaagaactt ccgtgtgtgc agaaatgtct
gaatatagca gtcatcttca 186600gtcagaatgc ttttctttct gctgtgtttc taccactaaa
ttgatagaaa tgaaatgagg 186660tgaagaaaaa aaaaaccact ctcctttgaa ggcctccatg
cttgactttc ttttgcttct 186720aaaagtgcag cagggcaatc gaggaggact ttatgtacta
tcattaatag gctacggctg 186780ccccttagag gtcaatttca aactctggat gtccacccag
gtgtcgtgag agtgaactgc 186840taatgtgaat tgcttaagaa ctcacctgct taaaataacc
acaatgcaaa attgaagctc 186900tagtgcctaa tttcaaactt cagtgttgaa atatatacag
gaatgcttga aactgctaat 186960accacttttc aaacagggaa taataatatt gctcttgcca
tactgtatgc tatagcactt 187020agaaaccact gcactgactt ggttcctgtt aggaagggag
gttttttatc agtttcccac 187080agagatgtca cacaaaaccc aagcttacat tctgcttaga
gttttttcct ctccctcctc 187140aggaggcaaa tccagtgctg tttctctggg tacgaggctc
agcctagttc tgagattacc 187200ctttcctttg cagacacaca tttatttttg aagactgcag
tttttgggat gcagatggct 187260attggaacaa gttgtaagat gtgagactgg ggaatgctgc
cttggctcat caagtaacac 187320gctgttagat gtgcaaccac aaacctcttc ccttacaaaa
ctaagtggct taaatttcta 187380ttttcatcct attgatgact agtcactgat gagctacaga
agtcaatgag taggctcaaa 187440taagcaatga aaaatccaaa gggcaaagct gaagttttaa
gctagttatt ttacagtctg 187500tccaggagta gttacttaaa cataccagta gtcttctgag
cattctgtga catctatttt 187560atctgtgact tttgcacttt gttgtgactc actggatgat
tctagcatgc agtgtgggct 187620tttctttgct tatccatcat tttcatgtgt cactgattgc
tgttgcaaaa tcatttccga 187680catattctgt tctcagagtt catggcagtc atttatagaa
ttgagtcaga aattcactgt 187740ctcaatggtc tttcctttaa aaagaaaaaa cggtgaaggt
aaggggaaga agggatttag 187800actccacaga aaaggaggaa aataatgtag acaaaagtaa
ctgatgctcc atgcaaaaat 187860ggagagagat gggggagaaa ctggtagtaa gaagacaaaa
gattaacctc tccatgtgcc 187920ttttaacaat tcaaagtgat gctaatactt tcctagcatt
tttagtggct tagtaaaata 187980tttttgttgc cctacgtcag agtaattaga gacacatgga
gtgaaatgaa aatatcgaag 188040ttgaagttat tttgtattta tttaaagcaa ggaatacagg
ctctgcttat taccaacttt 188100gtttagagct tgtcactact tctaaagtga gcaaatatgt
attcttgctc ctttacccta 188160aagcaaattt cacagatatc tccaattaac aattaaatct
cagggatcct tacttctcat 188220ctcttgcttt acgaaagagt gactgtgcta tactatgtta
tgcagtgtac ttagttctct 188280gtgcagtcaa atagtaaaaa gccctaagta actagatgcc
tgcttcatgt attaggactg 188340tcatgccagc ccagtagtaa ctcttagtgt ctctttcatt
ttagaatgaa accaaacctg 188400tgcagatgat gttcagaaaa ggtaaataca acatgaccta
tattggagac ttggagacca 188460aaatccttga gatcccttac attggtaatg aactcagtat
gatcgttcta ctccctgatg 188520caatccagga tgaatctact ggcttggaaa aggtaagtta
ttgagctcag tgcaaagaca 188580gtttgtgtcc tgccttggaa gagagtttgg tgctgcacat
ggattcacag ttcagtttca 188640gagctattat atcattgatg ctcaagactg actgaaatgc
tccttgtgtt tctgccccta 188700aagtggcatg ccatctatta ctactggcca agctatgtgc
tgctgtgcta agaggctctg 188760aaagaggcct catcagaagc tgtagttatg gtgaagccat
agtatgatga gcaccaaatg 188820agagggaatt tggggcagct cttaggaagt ccttaccaga
atttctacag tttgtcccat 188880aggtcatctt agtgaagacc tggcagattg tcactgcccc
tctacttgga aacacgctca 188940cagaatagtc caggttccct tccgttgtga tgatagaata
caagtcatgc tctggcctct 189000tgtttttttt tctaatgctg attttaattt aaaaagtgtt
gtaagcaggt tttgtcacca 189060gcccgtgagc tgaaagatcc tgaaaggctg aagaactggg
ttcagtttgt ttggggcctt 189120gtcagcagtt ctcccgtgcc tttactccct atatataaaa
taaggttttt acaatctgat 189180aatgttttat aaactgaact ttactgtatc taccacgaaa
aagaaaacac caaacaagaa 189240ttgacctcag ctgaagctgt agtctctagt aagtagaaac
ctgtagtgac ttgtgctttt 189300gacttgggat cctgtaagct cctgaaaaag atgcatattg
catgtatgtg tttacataac 189360acacatacac agacaaaagt agagattagt gcaaaactgt
cactattctt attttaatta 189420cctaatgttg ggttatgttt cgttgctttt tttgttttaa
gctggaaaga gaacttacat 189480acgagaagct gatggattgg atcaatcctg aaatgatgga
cagtacagaa gtgaggctgt 189540ctttacccag atttaaactg gaagaaaatt atgatctgaa
acccatcctg agcaacatgg 189600gaatgcgtga tgcgtttgac ttacggatgg cgaacttctc
aggaatctcc tctggtaacg 189660agcttgtgct ctctgaagtg gttcacaagt ccttcgtgga
ggtcaacgaa gaaggcactg 189720aagcagcagc tgccacagca ggagtgatgg tgctccgttg
tgctatgatc gttcccgact 189780tcactgccga tcatcccttc ctcttcttca tccggcacaa
caaaacttcc agtattttgt 189840tctgtggcag atattgctct ccctaagaag agagacagaa
gagctaccat taacgcagta 189900atgtgatttc ttttaggata gaactgctct tttgcactaa
ctgcttattt ccactgtgcc 189960tgaatcccct tatctggttg tcattttggg cttgcgtaga
gtaacaaagc cacttacaca 190020tacacagcag ctaccacttg aaacagctgc cttacacttt
gcacctaagt ggagttgttt 190080tcttgctggc ccaagaaaga tgaacatccc acttgctcag
tgaacttcca cctgtcttat 190140attttctatt gcactttgct tttgtgtggc caccaggtag
caaggtgaca aagagaaaag 190200aagtggattt tgtttctgac tatagtggaa gatatcttat
gctctgctcc ccatttttct 190260tcctctcccc acttattttt aactttttct ttaatgtttt
gataatagag ggagatgaaa 190320ggaggctttg gcgacctatt tgtaagagtt actaagcatc
tgcactagac agaggtttta 190380ttataactgg atagcactta cacaaggatg ggaataaaag
tatgtctgta acaaatgacc 190440ttagaggttt tcatggagta cggattctta tcttaacacc
acatgtgcca cctgggaata 190500ttagctatca ctcacctact tcattagtct tttaaaaaaa
gaatgttttt aaaaacaaac 190560aaacaaaaaa aaacccatag atgcctatgt agtatttaag
tgacagagct ttatttttgt 190620ttttcagtct ttatatgttt ttttcctatt ctgggtttgt
aaagcatctt tgttaatctg 190680aatgccaaag gtttcttaac gcagtgattt acgtgttttg
ctgttcttga aagaataaac 190740aaatttgttg tgagtgctgt gggcattgcc cataaatttt
gtggggtttt tttttttcat 190800ggctactgta aagaaaacaa gcaatcaact ttcgtgtagc
ttatgcagaa ttcattgctt 190860aacagaggct tttctgaatg ctgcaagacc aagatgctta
cctggattac gatggagttt 190920aggtttttac cttcgaagga ttcatagcaa ggagtctttg
aggcaaaggc tcaagggatt 190980ttaaagacta tctggttcca actccctgct gtgggcatgg
ctgcccaggg ctccctcctc 191040cctggccttc gacacctcca aggatggggc acccacagct
tccctgggca gctgtgccag 191100tgcctcacca acctccaagt gaaaaagatc cccctgacat
ctaattgaaa gttcctttca 191160tttattttaa agccattccc tcttattcca tcactatcag
accaaggctc cctttatgta 191220ttggaaggcc acaacaaggt ccccttggag tcttctcgag
gctgaacaag ctaagtgctt 191280gtttccagat ggggtactgc tgctgtgggt gcaactcctt
ggccccaggc caaggagtgt 191340gccatgcctc agatgcagcg attagtcacc atttggggtg
aggaagctgc caaagtgctg 191400cctgtcagac cgatgctcag tcagggctga gagcagcagt
gggtagaagg gaagtgggca 191460gcctctgctc ccagtgcatt gtctgggaag ggggtggtag
caagatgaaa agtagaaatt 191520tttctgaccc ttcctacgtg tccaggctgc tgctggagtg
tattcatggt gctatgctta 191580aagtgaaagc aaaagcgtgc ttgtctaatt tgcttctttt
ctaaattgaa aaggaaagta 191640atcacattaa cgtctaccat aaagcagaga gaagctgcca
gaaagcttga gagaagctag 191700aagcagccat atctacaaat cccagtgcaa acaagaagga
gggatcccag ctgcacaagc 191760aggaaggcag gaaggtttta cagcactgtc tgccgccagc
ctttgcgtaa ccatctgccc 191820gccccagcat tgcacctttc aacccactcc cagagacctc
acagctccca gtggtcctag 191880ctccagctta ctgctggctg ctctcctcct ggtttgatcc
tccctagcag ctgccaagca 191940tcacaggagg taagtgtgtg cttgctgtgc ctctgcattt
tgcagcctga aatgaatcca 192000gcccttggaa ctcgcactag ggcatcgagg agtgctttct
gaagccttca ctgaaacttt 192060tatttttcag ctgcagccat ggagagcctg agcaatgcca
acagcaggtt tgcacttgat 192120ctcttccgaa aggttaatga gacgaaccca tcaggaaaca
ttttcttctc ccctctcagt 192180atttctactg ctctggccat ggtcctcctc gggtccagag
gtaatacaga gacacaggtg 192240ctgaaggtca gcagcatttt cgcttgtttt attaaaatta
aatgttgttc agttttagag 192300acaaggcaag gggaggaggg cgttatttgc gtgagcttgg
ggcaaggttc ctgtcactcc 192360tgctgactct tcccccctgc tgccacctgc ctgctgcact
ccagagccct cctcttgtgc 192420tcactgatag cccttctttc tcacttcatt tgggttaatt
gatgaatctg gaaactaatt 192480tcactgattt atcagtctta atttaaaatc gattagcatc
tccagcagca agtctttact 192540agagcttgtg ataggacatg ggggaacagc attaaacaaa
aagggaagat ttaggcaaga 192600tatttgttag aggaagtttt tccactgaga aagtggtgag
gtggtggcac agctgcccag 192660ggaagctgag ggtgccccat ccctggaggt gttcaagacc
aggttggatg gggccctggg 192720cagcctgagc tggtgggtgg ctgccctgcc cacagcaagg
cagtggaact gggtgggctt 192780taagttgaat tccagcccaa ccccattcta tgattctatg
agccttttcc acagagaact 192840attgttttgc aatgtataca tacataatgg tatacatagt
aatgctaagt gtatcttata 192900aataaaaaat aaaatataga gctgtattat tctaaggctg
acaactgtta caatacatgg 192960tgatgttacc caagacccag tgttatagca gccaagcacc
cagtatttct gaggagcaga 193020actcacgtgt ccattctcat ggtatcctta ggttgagcag
cagaggttaa atgaaaatgg 193080tgtggctcct ttactggggg ctttgttgtg gacccagctc
atcaatcctt tcccactctc 193140cacaacagca gttgcaaact gcaaattctc atgtaggtag
cagtgccaat tccctctcag 193200actcatgttc aaaagggacc ctgcctcttt tttaatttgc
aaggcaaaca cctgctagtg 193260caaggggaag tatgaaagaa ttgtcgctgt agttcctatt
aacttatttg ccctatgatt 193320aagttcacct ttgtattccg aactttagga agaacttgtt
tagaccatta actgctgcca 193380ttctttgtga aaagactata aaactgaatc actgcttgta
gaaacagact ttgaacatac 193440attccttata actcaactgt cagccccacc caggaagaat
ctactgagag cagaaataat 193500gcaagagaag catagggaag ttggagatag aaggttggga
tgaatggttg gactgggtga 193560tcctgtgtgt cttttccaac ttcagtgatt ctatgattct
aaggtgtttc agcacagtaa 193620ccttctgtaa tgcacattcc catggtataa tgtttaattg
atgagaacat cagttaatta 193680aggagatgat gactgatgag tgtgaagggt gtttataagc
atgcagaaat ccatttctgg 193740gatcataatc ctaccttaag ttggaatcat agagtacacc
acggtggagg ggatccatga 193800ggatccagct ccacacagca ccacccacta tggtagatcc
tgctgcccaa cctgcacacc 193860ttggctagtc agcttccctt caggtatctg tatgcacgct
ttcatattat aacagctttt 193920aattttaagg tgatagttgt ctgtagaagc acttatattt
tcataaaacc aaaggttata 193980gctctcacat tttcctaaca cctcaccttc cctgagtgct
cagacaagct cagtagtcca 194040cggaggaaaa acatgcagac agcaccctat taggactctg
gatcacaatt aacagcttca 194100gctgtggcta actgtattca gctactgctt tacaagtgac
atggctggca cagcactaag 194160ggacagtttc acttgttctt tgatggttac agctttcagc
ttctttctgc ttttgttttt 194220caacttaact accaaacaaa taccatacag atatgctgca
tgttctctat aaatacagca 194280ttagcagtag ttagctcatc tctttcattt cagacgtttc
attttgatga agttgaaaat 194340atacactcaa gattccgggc tctgactgca gacatcaaca
gaagggattc ttcctgtctc 194400ctacggattg ccaaccggct ttatggagag aagtcctaca
gctttctgcc ggtatgggta 194460cacagaccat agctgtgtgg tggaacctgg ggggaggctt
tgtaacttca tcatctgttg 194520ctctcctgcc tccagaacgc gccccatagc aaaaatatca
caccagcaag tccagatgtc 194580aaaactatct ttctgcatca ataagcagca tagctcaggt
gttgctgtct ttataggaat 194640gcagccattt gagtatttga ggtaaaaaca tgactagaca
tctaaaagtt accaggcagt 194700cagtacgagt gttgtacaca tgcctataga tgcagaaatg
catatgcatc tggacatcct 194760aaaggatacg cctagaggat attacataac aaatcccttt
ctttgatagt tcagttctgc 194820tgctttgggg ctcaagagaa attgcaagcc atgtaggttc
ttagcttaga gtacagatta 194880gcaatgcccc attcctctgt ctgttgtttt ttaggctttt
cattgctcta gtactatatt 194940acttaaaaca tttttgaaaa catttctctg gggggagatt
gccatcatgt ctcaacagca 195000tgcctcttta caagggaact gtacctctgc atctatttag
gtactgctat ttttatccct 195060ctccagctct ttctgggagt ttttgttttc ttagtcaagc
tt 1951022285DNASV40 2aaagtctaga gtcggggcgg ccggccgctt
cgagcagaca tgataagata cattgatgag 60tttggacaaa ccacaactag aatgcagtga
aaaaaatgct ttatttgtga aatttgtgat 120gctattgctt tatttgtaac cattataagc
tgcaataaac aagttaacaa caacaattgc 180attcatttta tgtttcaggt tcagggggag
gtgtgggagg ttttttaaag caagtaaaac 240ctctacaaat gtggtaaaat cgataaggat
ccgtcgagcg gccgc 2853498DNAArtificialHuman interferon
alpha2b codon optimized for avian expression 3tgcgatctgc ctcagaccca
cagcctgggc agcaggagga ccctgatgct gctggctcag 60atgaggagaa tcagcctgtt
tagctgcctg aaggataggc acgattttgg ctttcctcaa 120gaggagtttg gcaaccagtt
tcagaaggct gagaccatcc ctgtgctgca cgagatgatc 180cagcagatct ttaacctgtt
tagcaccaag gatagcagcg ctgcttggga tgagaccctg 240ctggataagt tttacaccga
gctgtaccag cagctgaacg atctggaggc ttgcgtgatc 300cagggcgtgg gcgtgaccga
gacccctctg atgaaggagg atagcatcct ggctgtgagg 360aagtactttc agaggatcac
cctgtacctg aaggagaaga agtacagccc ctgcgcttgg 420gaagtcgtga gggctgagat
catgaggagc tttagcctga gcaccaacct gcaagagagc 480ttgaggtcta aggagtaa
498
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