Patent application title: Novel apolipoprotein gene involved in lipid metabolism

Inventors: Edward Rubin (Berkeley, CA, US) Len A. Pennacchio (Sebastopol, CA, US)
Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
IPC8 Class: AA61K3817FI
USPC Class: 514 12
Class name: 25 or more peptide repeating units in known peptide chain structure
Publication date: 07/16/2009
Patent application number: 20090181884

Abstract:

Methods and materials for studying the effects of a newly identified human gene, APOAV, and the corresponding mouse gene apoAV. The sequences of the genes are given, and transgenic animals which either contain the gene or have the endogenous gene knocked out are described. In addition, single nucleotide polymorphisms (SNPs) in the gene are described and characterized. It is demonstrated that certain SNPs are associated with diseases involving lipids and triglycerides and other metabolic diseases. These SNPs may be used alone or with SNPs from other genes to study individual risk factors. Methods for intervention in lipid diseases, including the screening of drugs to treat lipid-related or diabetic diseases are also disclosed.

Claims:

1. An isolated APOAV polypeptide having the amino acid sequence set forth in SEQ ID NO: 7.

2. A composition for lowering plasma triglycerides comprising a polypeptide having at least 70% homology to the polypeptide of claim 12 and a pharmaceutically acceptable excipient.

3. An antibody that specifically binds to a polypeptide according to claim 12.

4. The antibody of claim 3, wherein the antibody specifically binds to an epitope comprising position 19 of SEQ ID NO:7.

5. A polynucleotide encoding the polypeptide according to claim 1.

6. The composition of claim 2, wherein the polypeptide has at least 95% homology to the polypeptide of claim 12.

Description:

CROSS-REFERENCES TO PRIOR APPLICATION

[0001]This application is a divisional of U.S. patent application Ser. No. 10/229,834, filed Aug. 27, 2002 which claims priority to Application No. 60/315,210, which was filed on Aug. 27, 2001, the disclosure of which are hereby incorporated by reference in their entirety for all purposes.

REFERENCE TO SEQUENCE LISTING

[0003]Applicants assert that the paper copy of the Sequence Listing is identical to the Sequence Listing in computer readable form found on the accompanying computer disk. Applicants incorporate the contents of the sequence listing by reference in its entirety.

BACKGROUND OF THE INVENTION

[0004]1. Field of the Invention

[0005]This invention generally relates to human lipid metabolism, particularly to apolipoproteins, genes encoding these apolipoproteins, related proteins, and their mutations and polymorphisms as they relate to cardiovascular, coronary and other diseases.

[0006]2. Description of the Related Art

[0007]Cardiovascular diseases are the number one cause of death in Western societies. Studies repeatedly show that individuals with high levels of very low-density lipoprotein, (VLDL) and/or low levels of high density lipoprotein (HDL) have significantly increased chances of developing cardiovascular disease. It has been established that strategies to reverse the levels of these lipoprotein particles will lower disease risk in susceptible individuals.

[0008]Lipoproteins function as transport vehicles for triacylglycerols (triglycerides), cholesterol and other lipids. These complexes solubilize highly hydrophobic lipids, and regulate entry and exit of particular lipids at specific targets. Lipoproteins form micelle-like particles that consist of a nonpolar core of triacylglycerols, more commonly known as triglycerides, and cholesteryl esters surrounded by a coating of protein, phospholipids, and cholesterol. The lipoproteins are classified according to density. Lipoprotein particles are composed of lipids and proteins and are such particles as chylomicrons, very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL). (Voet and Voet, Biochemistry, 1990; Stryer, Biochemistry, 1995). The protein components of lipoproteins are known as apolipoproteins.

[0009]Van der Vliet, H N et al., report on a gene that shares homology to APOAV and note its increase in expression following rat liver hepatectomy in J Biol Chem. 2001 Nov. 30; 276(48):44512-20. The rat (GenBank Accession Nos. AF202888 and AF202887), mouse (GenBank Accessions No. AF327059) and human (GenBank Accessions Nos. AF202890 and AF202889) versions of these sequences were deposited in GenBank and entitled, Regeneration-associated protein 3 (Rap 3) mRNA, complete cds. Rap3 is noted in GenBank as an "apolipoprotein-like serum protein; concentration elevated after a 70% partial hepatectomy" in rats.

[0010]The human genomic region containing the DNA sequence for APOAV was sequenced by the Human Genome Project and deposited in GenBanK under Accession Numbers AC007707 and/or AC074203. These deposits cover approximately 200 kb of human genomic DNA. The deposits are associated with clustered 11q23 and 22q11 breakpoints, but no coding regions are described. Computational analyses indicate the previously described APOA-I, APOC-III, and APOA-IV are contained within this interval.

[0011]The GenBank Accession Number AC007707 sequence shows the opposite strand (reverse complement) of the sequences of the present invention. The reverse complement of AC007707 was used as the starting point of the present invention for finding the APOAV gene and coding sequence.

[0012]Yen et al., in PCT Publication WO 01/007803, entitled "Apolipoprotein A-IV-related protein: Polypeptide, polynucleotide sequences and bi-allelic markers thereof" describe a gene corresponding to the present APOAV. The gene is described as encoding an apolipoprotein A-IV-related protein (AA4RP) as well as regulatory sequences at the 5' and 3'end. Also disclosed are biallelic markers of the AA4RP gene useful in genetic analysis. However, Yen et al. describe their biallelic markers differently than the SNP's of the present invention. They disclose no description of any known linkages between these markers and any known disease phenotype.

[0013]Several human cDNA sequences derived from the APOAV gene have been previously disclosed in GenBank. A sequence file generated by the NCBI annotation project in July 2001 (transcript version 1) is disclosed as human mRNA/cDNA sequence, XM_--052110. A second sequence file generated by the NCBI annotation project identifies in July 2001 (transcript version 2) can be found as XM_--052109. AF202890 (called RAP3) is a third cDNA sequence that is related to the van der Vliet et al. Human ortholog of rat liver regeneration associated protein (transcript version 1). AF202889 (called RAP3) is the fourth cDNA sequence related to the van der Vliet et all. No. AF401201 and was made public on 7 Oct. 2001.

[0014]Other related sequences include mouse mRNA/cDNA sequence (AF327059), called RAP3, which is the sequence identified as the mouse ortholog of rat liver regeneration associated protein. Three publicly generated mouse full-length cDNAs for apoAV are found under the following Accession Nos.: AK004903 (transcript version 2), BC011198 (transcript version 2), and AK004936 human ortholog of rat liver regeneration associated protein (transcript version 2).

[0015]Human protein sequences were predicted from mRNA sequences AF202890 and AF202889 and called AAF25662 and AAF25661, which correspond to the APOAV protein, which can be found under NP_--443200 in NCBI.

[0016]The mouse genomic region (which includes additional genes sequenced and used to create the knock-out mice described herein) is SEQ ID NO: 7 in this application. This sequence was deposited in GenBank under GenBank Accession (transcript version 1). The mouse RAP3 protein (GenBank Accession No. AAG49600) is the protein sequence predicted from mRNA sequence AF327059.

BRIEF SUMMARY OF THE INVENTION

[0017]The present invention involves a human apolipoprotein gene and its expressed product, Apolipoprotein A-V (herein referred to as "APOAV" or "APOA5"), located near a previously described apolipoprotein AI/CIII/AIV gene cluster (a region repeatedly implicated in various cardiovascular diseases) and its association with elevated levels of triglycerides.

[0018]The various aspects of the invention, as described below, are useful in the genetic analysis of cardiovascular disease. Patients with genetic predispositions to certain conditions may be screened with the analyses provided herein. High levels of APOAV protein expression are associated with lowered triglyceride levels, and low levels of APOAV expression (as demonstrated in "knock-out mice") are associated with increased levels of triglycerides. Furthermore, various polymorphisms have been identified which are correlated with different plasma triglyceride levels. Specifically, individuals with minor alleles for several SNPs near APOAV consistently display increased plasma triglyceride levels.

[0019]In addition, the present invention involves identification of a strong association between uncommon alleles of SNPs in the APOAV gene and increased plasma triglyceride levels in the general human population. Thus the present invention enables genetic testing for APOAV variants and their correlation to increased triglyceride levels in people having APOAV polymorphisms deviating from the normal or "wild type" phenotype. Further, a combination test APOCIII is suggested. Genetic testing may be carried out on a patient's DNA or RNA or protein, provided that antibodies capable of distinguishing mutant from wild type APOAV protein are available. Furthermore, genetic testing using the markers disclosed herein may be used to identify individuals at risk for diabetes and/or insulin resistance. Genetic testing may also be used to determine an individuals' susceptibility to Familial hypercholesterolemia or other forms of hypercholesterolemia.

[0020]The invention also provides means for identifying haplotypes that are linked to the diseases of hyperlipidemia (CHL) and familial combined hyerlipidemia (FCHL).

[0021]Association studies indicate the existence of three haplotypes (APOA5*1, APOA5*2, APOA5*3) in APOA present in the general human population, that are associated with triglycerides. These three haplotypes are composed of five biallelic markers (SNPs 1-3, 5, and 6).

[0022]Thus, the invention includes using various methods for screening for genetic APOAV haplotypes or SNPs in humans. Fragments of various lengths of APOAV SEQ ID NOS: 1-7 may be placed onto solid supports for use in gene chips or other parallel formats for assay purposes. The sequences used will span a SNP and have sufficient flanking bases for specificity and binding, e.g. 10 bases on either side (5' and 3') of the nucleotide bearing the SNP. As few as 2 and as many as 1,000 bases may be used, depending on test design considerations.

[0023]Other methods for diagnostic purposes in this invention include but are not limited to, making antibodies to APOAV and its variants, attachment of the APOAV sequences disclosed herein onto solid supports for array and gene chips, and other hybridization assays.

[0024]The invention provides non-human animals that over-express the human version of APOAV. The over-expression of this gene results in these animals having dramatically reduced plasma triglyceride levels (˜3-fold). In addition to decreased triglyceride levels these mice also have corresponding decreases in VLDL levels.

[0025]The invention also provides homozygous knockout non-human animals that are lacking apoA5 and therefore do not produce apoA5 protein. These animals have increased and VLDL and triglyceride levels. This invention also includes recombinant vectors and DNA targeting constructs, such as the one used by the inventors to delete mouse apoA5 and was built using PCR products and primers made from SEQ ID NO: 7.

[0026]This invention also provides non-human animals for further animal studies by pharmaceutical companies to study human or mouse apoA5. Animal studies that explore the regulation and expression of human or mouse apoA5, its interaction with other apolipoproteins, production of antibodies for mutant and wild-type apoA5, and further in vivo study of apoA5. For example, mice lacking wild-type apoA5 may be exposed to various test substances to determine the triglyceride lowering effect of the test substance on individuals having a non-wild-type apoAV gene. The invention provides non-human animals useful for studying apoCIII since its levels are altered in these mouse models.

[0027]The invention can be further characterized as including an isolated polypeptide wild type APOAV protein as set forth in SEQ ID NO: 4, which corresponds to the ideal normal APOA5*1 haplotype. One mutant protein is encoded by DNA carrying the uncommon SNP5, described below as variation SNP5 at position 12974 is set forth in SEQ ID NO: 3 and corresponds to haplotype APOA5*3.

[0028]Another aspect of the invention is that stratification of populations based on APOA5 markers may identify a subset of individuals that respond differently to current and future drug therapies. These studies would contribute in understanding which of these drug therapies or combinations of drug therapies are the most beneficial to lower triglyceride levels in individuals having haplotypes APOA5*1, APOA5*2 or APOA5*3.

[0029]The SNPs disclosed herein can also be studied for association with other diseases including, but not limited to, diabetes, obesity, metabolic syndrome, or other generic disorders. The inter-relatedness of these conditions is well established in the literature. APOA5-increased expression or other means for protein delivery may prove to be successful to treat numerous symptoms of these diseases.

[0030]This invention also provides the means of combination therapy which uses high levels of APOAV expression or protein regardless of genotype or haplotype to treat any condition of high triglycerides. This strategy could also be combined with stratification-based studies. A further aspect of the invention is gene therapy to deliver active drugs to liver cells to over-express APOAV and thereby decrease triglycerides. Delivery of APOAV therapies can be by such methods as but not limited to, injection of active APOAV, delivery by pill form, and inhalation by spray to deliver APOAV to lungs and the blood to reduce triglycerides.

[0031]The inventions also encompasses drug screening and design of therapeutic agents to be used in methods for increasing APOAV expression, and thereby lowering triglycerides, based on the APOAV polynucleotides and polypeptides described herein is also an important aspect of the invention, especially in the identification of genes, regulatory elements, ligands, drugs and other therapeutic agents to be used to modulate and regulate APOAV expression. Such therapeutic agents include current drug therapies for high triglyceride levels such as fibrates or other drug agents which are known to reduce triglycerides.

BRIEF DESCRIPTION OF THE DRAWINGS

[0032]FIG. 1. A diagram showing the genomic organization of the human APOAI/CIII/AIV cluster (1a) and VISTA plot showing similarities between the mouse and human sequences in this region.

[0033]FIG. 2. A diagram of the targeting construct used to generate apoA5-deficient mice. Homology arms were designed to delete the coding exons of the gene (depicted by black boxes).

[0034]FIG. 3. Bar charts showing plasma triglyceride and cholesterol for human transgenic (FIG. 3A) mice and apoAV knockout mice (FIG. 3B).

[0035]FIG. 4. (A) New SNP map of the APOAV genomic locus. Exons are depicted by rectangles with coding sequence filled in with black and untranslated regions with white. The gene is transcribed in the right to left direction. (B) Minor allele frequencies are approximately 10% in Caucasians for SNPs 1, 2, 3, 5, 6, and ˜40% for SNP4. Minor alleles for SNPs 1, 2, 3, and 6 form a common haplotype (˜10%). SNP5 is part of a second independent haplotype (˜10%).

[0036]FIG. 5. Table of genotyping data from 501 individuals (A); pair-wise measures of linkage disequilibrium (B); and SNP3 genotyping data from a different set of individuals stratified by triglyceride levels (C).

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

Definitions

[0037]The term "triglycerides" is used in its ordinary medical sense. However, the tests of the present invention for disposition towards elevated triglyceride levels may also include elevated triacylglycerol, cholesterol, and other related lipid levels, very low density lipoprotein (VLDL) levels, or levels of other closely related apolipoproteins or lipoprotein particles such as chylomicrons, intermediate density lipoprotein (IDL), or low density lipoprotein (LDL) or high density lipoprotein (HDL) levels.

[0038]"Single nucleotide polymorphisms" (SNPs), which are defined in relation to a population, are variations in DNA at a single base that are found in at least 1% of the population. The terms "biallelic marker," "marker," "polymorphism" and "allele" are also used to denote variations at a single base and are used interchangeably.

[0039]The term, "genotype," is used herein to mean a specific allele or alleles an individual carries at a given locus. It can also be used to describe a set of alleles for multiple loci.

[0040]A "haplotype" is a set of alleles for closely spaced polymorphisms along a chromosome that tend to be inherited together. Alternatively a haplotype can be thought of as a combination of alleles of closely linked loci that are found in a single chromosomal interval and tend to be inherited together. An individual SNP allele can be used to define a given haplotype.

[0041]The term "phenotype," is used herein to mean the form taken by some character (or group of characters) in a specific individual. It can also mean the detectable outward manifestations of a specific genotype.

[0042]The term, "proband" is used to mean an affected individual in a family.

[0043]The term, "allele" is used herein to mean one of the different forms of a gene that can exist at a single locus. An allele is also used to describe a given version of a polymorphism.

[0044]The term, "allele frequency" is used to mean a measure of the commonness of an allele in a population, the proportion of all alleles of that gene or polymorphism in the population that are of this specific type.

[0045]The term, "Hardy-Weinberg" is used to refer to calculating the Hardy-Weinberg equilibrium for genotypes, whereby the stable frequency distribution of genotypes AA, Aa, and aa, in the proportions p², 2pq and q², respectively (where p and q are the frequencies of the alleles A and a), that is a consequence of random mating in the absence of mutation, migration, natural selection or random drift.

[0046]The term "P-value" is used herein to mean the probability that the results were not significant. For example, a p-value of 0.05 means that there are 5 chances in 100 that the results are not significant.

[0047]The term, "SEM" is used to mean the standard of the mean.

[0048]The term "linkage disequilibrium" is used herein to refer to the relationship that is said to exist between a allele found at a single polymorphic site and alleles found at nearby polymorphisms if the presence of one allele is strongly predictive of the alleles present at the nearby polymorphic sites. Thus, the existence of linkage disequilibrium (LD) enables an allele of one polymorphic marker to be used as a surrogate for a specific allele of another.

[0049]"Substantial homology or similarity" means that a nucleic acid or fragment thereof is "substantially homologous" (or "substantially similar") to another if, when optimally aligned (with appropriate nucleotide insertions or deletions) with the other nucleic acid (or its complementary strand), using BLASTN there is nucleotide sequence identity in at least about 60% of the nucleotide bases, usually at least about 70%, more usually at least about 80%, preferably at least about 90%, and more preferably at least about 95-98% of the nucleotide bases. To determine homology between two different nucleic acids, the percent homology is to be determined using the BLASTN program "BLAST 2 sequences". This program is available for public use from the National Center for Biotechnology Information (NCBI) over the Internet (Altschul et al., 1997). The parameters to be used are whatever combination of the following yields the highest calculated percent homology (as calculated below) with the default parameters shown in parentheses: [0050]Program--blastn [0051]Matrix--0 BLOSUM62 [0052]Reward for a match--0 or 1 (1) [0053]Penalty for a mismatch--0, -1, -2 or -3 (-2) [0054]Open gap penalty--0, 1, 2, 3, 4 or 5 (5) [0055]Extension gap penalty--0 or 1 (1) [0056]Gap x_dropoff--0 or 50 (50) [0057]Expect--10

[0058]The terms "substantial homology" or "substantial identity", when referring to polypeptides, indicate that the polypeptide or protein in question exhibits at least about 30% identity using BLASTP with an entire naturally-occurring protein or a portion thereof, usually at least about 70% identity over the common lengths, more usually at least about 80% identity, preferably at least about 90% identity, and more preferably at least about 95% identity.

[0059]Homology, for polypeptides, is typically measured using sequence analysis software. See, e.g., the Sequence Analysis Software Package of the Genetics Computer Group, University of Wisconsin Biotechnology Center, 910 University Avenue, Madison, Wis. 53705. Protein analysis software matches similar sequences using measures of homology assigned to various substitutions, deletions and other modifications. Conservative substitutions typically include substitutions within the following groups: glycine, alanine; valine, isoleucine, leucine; aspartic acid, glutamic acid; asparagine, glutamine; serine, threonine; lysine, arginine; and phenylalanine, tyrosine.

[0060]The term "polynucleotide" refers to a chain of nucleotides without regard to length of the chain.

[0061]The term "polypeptide" refers to a polymer of amino acids without regard to the length of the polymer; thus, peptides, oligopeptides, and proteins are included in this term.

[0062]A. Sequences of Apolipoprotein A-V

[0063]Despite the previous availability of sequence in the human apoAI/CIII/AIV genomic interval, the gene APOAV was characterized by human/mouse sequence comparison, using the power of comparative sequence analysis to prioritize potential functional regions of the genome. APOAV represents a fourth member of the clinically important apolipoprotein gene cluster on human 11q23. The human and mouse data, both when taken independently and combined, indicate an important role for APOAV in plasma triglyceride homeostasis. While previous data have associated the apoCIII locus with extremely high plasma triglyceride levels in humans, the results of the present studies suggest the possible use of APOAV polymorphisms as prognostic indicators for hyper-triglyceridemia susceptibility and the focus on APOAV modulation as a potential strategy to reduce this known cardiovascular disease risk factor.

[0064]FIG. 1 shows human and mouse comparative sequence analysis of the apoAI/CIII/AIV gene cluster. (A) A schematic of the genomic organization of human APOAV and the relative SNP positions (arrows). APOAV exons are shown with solid boxes and the distance between each SNP is indicated above the line. The predicted transcription start site is depicted by a bent arrow and the relative position of the promoter, and start and stop codons are shown. (B) In each panel 30 kbp of contiguous human sequence is illustrated horizontally. Above each panel arrows correspond to known genes and their orientation with each exon depicted by a box (gene names are indicated above each arrow). The VISTA (VISualization Tools for Alignment) plot displays the level of homology between human and the orthologous mouse sequence. Human sequence is represented on the x-axis and the percent similarity with the mouse sequence is plotted on the y-axis (ranging from 50-100% identity).

[0065]A preferred embodiment involves a human apolipoprotein gene, APOAV, located near a previously described apolipoprotein AI/CIII/AIV gene cluster (a region repeatedly implicated in various cardiovascular diseases). Electronic homology searches with human apoAI, apoCIII, and apoAIV mRNA sequences using the BLAST algorithm (S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, J Mol. Biol 215, 403-10 (1990)) identified a genomic bacterial artificial chromosome (BAC) clone containing the complete apoAI/CIII/AIV gene cluster (GenBank Accession No. AC007707).

[0066]The predicted 368-amino acid sequence shows significant homology to various known apolipoproteins, with the strongest similarity to mouse apoAIV (24% identity and 49% similarity). Examination of the orthologous human genomic sequence indicates a similar genomic structure to the mouse region and predicts an open reading frame encoding a 366-amino acid protein with high sequence homology to mouse apoAV (71% identity and 78% similarity), as well as human apoAIV (27% identity, 48% similarity). Protein structure analyses predicts several amphipathic helical domains and an N-terminal signal peptide in both human and mouse APOAV, which are characteristic features of lipid-binding apolipoproteins.

[0067]Transcripts approximately 1.3- and 1.9-kilobases (kb) in length were identified predominantly in liver tissue from both species by Northern blots analysis, where mRNA from several different human and mouse tissues was hybridized with APOAV cDNA probes from human and mouse, respectively. The full-length sequences of mouse cDNAs indicate the two transcripts in mice are likely the result of alternative polyadenylation. The mouse apoA5 cDNA sequences are available under GenBank Accession Nos. AK004936 and AK004903.

[0068](1) Brief Description of the Sequences (SEQ ID NOS: 1-48)

[0069]SEQ ID NO: 1 and 2 are cDNA sequences corresponding to the coding sequence of a "wild type" APOAV gene and are deposited in GenBank under Accession No. AF202889.1 and F202890.1. Both cDNAs contain the normal wild type alleles. SEQ ID NO: 1 is a 1.3 kb transcript of the APOAV gene. SEQ ID NO: 2 is an alternatively spliced 1.8 kb transcript of the APOAV gene. The protein is encoded on the reverse complement. SNP5 is the only SNP in the group that changes an amino acid (Serine (S 19)→Tryptophan (W19)) at position 19 of the putative protein. The substitution of G for A in SNP6 is in a critical nucleotide of the Kozak consensus sequence (-3 bp).

[0070]SEQ ID NO: 3 is the human genomic sequence comprising the present wild type APOAV gene, certain of its regulatory elements, and the SNPs associated with the genomic sequence. The indicated SNPs are numbered as follows: SNP 4, 3, 6, 5, 2, 1 in the order in which they appear in the sequence. The following table indicates the base pair positions in SEQ ID NO: 3 where these polymorphisms are found. Profiles for each of these SNPs can be found in GenBank under the following Accession Numbers: 2266788 (SNP1), 2072560 (SNP2), 662799 (SNP3), 3199916 (SNP4), 3135506 (SNP5), 651821 (SNP6) and 3135507 (V153M).

TABLE-US-00001 TABLE 1 SNPs shown in SEQ ID NO: 3 SNP Position Number Original allele > number in SEQ ID NO: 3 Rare allele Location in APOA5 4 567 T > C Between APOA5 and APOA4 3 11674 T > C Upstream 6 12802 A > G 5' untranslated region 5 12974 C > G causing an amino acid change in the APOA5 gene product (S19 → W19) 2 13555 G > A Intervening sequence 3 + 476 1 14695 T > C Coding sequence 1259

[0071]SEQ ID NO: 3 and 4 are annotated to show certain regulatory regions (CAAT box and TATAA box); the exons; and start and stop codons and the untranslated regions.

[0072]SEQ ID NO: 4 is the ideal wild type genomic sequence of human APOAV gene and contains the alleles in their major form, as do the corresponding GenBank sequences.

[0073]SEQ ID NO: 5 is the human DNA sequence used to create the transgenic mice expressing human APOAV. This sequence has not been deposited in GenBank.

[0074]SEQ ID NO: 6 is the working draft sequence of the mouse apoA5 that was deposited by the inventors in GenBank and recently released for publication to the public. It is the mouse genomic apoA5 region used to generate the homozygous knock-out mice. It consists of 75 unordered and unoriented contigs, wherein the gaps of unknown length are denoted as an `n` in the sequence.

[0075]SEQ ID NO: 7 is the amino acid sequence of the protein product generated from SEQ ID NO: 4. A suitable wild-type APOAV protein is set forth in SEQ ID NO: 7. One mutant protein is encoded by DNA carrying the uncommon SNP5, is set forth in SEQ ID NO: 3.

[0076]SEQ ID NOs: 8-9 are the forward and reverse primers used to isolate mouse genomic DNA in Example 1 from the pooled mouse BAC library.

[0077]SEQ ID NOs: 10-11 are the forward and reverse primers used to genotype transgenic mouse for the human APOAV gene.

[0078]SEQ ID NOs: 12-15 are the PCR primers that were used to build the homology arms in the targeting construct to delete mouse apoA5 in knockout mice.

[0079]SEQ ID NOs: 16-17 were used to amplify the external 3' probe when creating the apoA5 knockout animals.

[0080]SEQ ID NOs: 18-19 and SEQ ID NOs: 37-38 are the primers used to genotype the apoA5 knockout animals. SEQ ID NOS: 18-19 are the forward and reverse primers to genotype for the presence of the apoA5 gene.

[0081]SEQ ID NOs: 20-21 are the forward and reverse primers used to amplify and genotype SNP3.

[0082]SEQ ID NOs: 22-23 are the degenerate primers used to genotype the transgenic animals for the presence of APOAV and to probe PCR amplified liver cDNA for human or mouse APOAV cDNAs.

[0083]SEQ ID NOs: 24-25 are the forward and reverse primers used to genotype SNP5. SEQ ID NOs: 26-27 are the reverse and forward primers used to genotype the V153M polymorphism.

[0084]SEQ ID NOs: 28-36 are the probes and INVADER sequences used to perform the INVADER assays to genotype SNPs 5, 6 and V153M.

[0085]SEQ ID NOs: 37-38 are the forward and reverse primers used to genotype the presence of the neomycin gene in preparing the apoA5 knockout mice.

[0086]SEQ ID NOs: 39-48 are primers used to genotype the six SNPs as shown in Table 3.

[0087]SEQ ID NOs: 39-40 are the forward and reverse primers used to amplify or genotype SNP1.

[0088]SEQ ID NOs: 41-42 are the forward and reverse primers used to amplify or genotype SNP2.

[0089]SEQ ID NOs: 43-44 are the forward and reverse primers used to amplify or genotype SNP5.

[0090]SEQ ID NOs: 45-46 are the forward and reverse primers used to amplify or genotype SNP3 and SNP6.

[0091]SEQ ID NOs: 47-48 are the forward and reverse primers used to amplify or genotype SNP4.

[0092](2) Applications for APOAV Sequences

[0093]In another embodiment, a polynucleotide fragment is also contemplated wherein the fragment comprises a contiguous span of at least 12 nucleotides of SEQ ID NO: 3, where said contiguous span encompasses one or more SNPs 1-3, 5 or 6 as described in SEQ ID NO: 3.

[0094]A further preferred embodiment consists of a purified, isolated, synthesized or recombinant nucleic acid that hybridizes with an SNP nucleotide-containing the nucleotide sequence of SEQ ID NOs: 3 or 5, or a complementary sequence or a variant that is substantially homologous.

[0095]B. APOAV DNA Constructs and Recombinant Vectors

[0096]The present embodiment encompasses a recombinant vector comprising a polynucleotide that is substantially homologous to any of the polynucleotides described herein, including regulatory sequences, coding sequences and polynucleotide constructs, as well as any APOAV primer or probe. In a first preferred embodiment, a recombinant vector comprises expression vectors comprising either a regulatory polynucleotide of APOAV or a coding nucleic acid of the present embodiment, or both. Within some embodiments, the expression vectors are employed in the in vivo expression of APOAV in non-human animals. In other embodiments, the expression vectors are used for constructing transgenic animals and gene therapy.

[0097]Depending on the host organism or cell wherein the APOAV gene will be expressed, one skilled in the art can adapt the recombinant vector to further comprise genetic elements, including but not limited to, an origin of replication in the desired host, suitable promoters and enhancers, any necessary ribosome binding sites, polyadenylation signal, splice donor and acceptor sites, transcriptional termination sequences, selectable markers and non-transcribed flanking sequences. Various types of gene delivery vectors can be used including, but definitely not limited to, plasmids, YACs (Yeast Artificial Chromosomes), BACs (Bacterial Artificial Chromosomes), bacterial vectors, bacteriophage vectors, viral vectors (for example, retroviruses, adenoviruses and viruses commonly used for gene therapy), non-viral synthetic vectors, and recombinant vectors, etc.

[0098]A second embodiment comprises a host cell that has been transformed or transfected with one of the APOAV polynucleotides described herein, in particular a polynucleotide comprising SEQ ID NO: 1, 2 or 5 or a fragment or variant thereof. Appropriate host cells can be prokaryotic host cells, such as E. coli, Bacillus subtilis, Salmonella typhimurium, and strains from species including but not limited to, Pseudomonas, Streptomyces and Staphylococcus. Alternatively eukaryotic host cells can be used, including but not limited to, HeLa cells, HepG2 and other mammalian host cells. A preferred embodiment is a mammalian host cell comprising the APOAV genomic region, wherein the APOAV gene is disrupted by homologous recombination with a knockout vector.

[0099]In order to study the physiological and phenotypic consequences of a lack of synthesis of the APOAV protein, both at the cellular level and at the organism level, the preferred embodiment also encompasses DNA constructs and recombinant vectors enabling conditional expression of a specific allele or haplotype of the APOAV genomic sequence as described in SEQ ID NO: 3, 5 or 6 or an APOAV cDNA (SEQ ID NO: 1, 2) in a transgenic non-human animal. The embodiment also encompasses DNA constructs to generate animals having multiple copies of the APOAV protein expressed and animals having no APOAV protein that is expressed ("knock-out animals").

[0100]The targeting construct can be built by various methods known in the art including but not limited to, PCR primers for integration by homologous recombination, using a represssor/marker promotor construct, Cre-LoxP system, and antisense constructs. The method preferred is using PCR products and primers to build the targeting construct. To build such a construct to make knockout non-human animals and cells, one would need the homology "arms" that flank each side of the sequence to be deleted or disrupted, and a selectable marker inserted between the arms to select for the marker function. The sequence to be deleted can be the whole APOAV gene as the inventors did in Example 3, single or multiple exons, intervening genomic sequences, short peptide sequences and even single base pair deletions. After delivery of the construct into embryonic stem cells, selection for the marker permits gene deletion. Or for instance, APOAV gene function can be disrupted by insertion of the selectable marker, by inserting insertion of the marker in the promoter, splice sites, or the open reading frame.

[0101]To make transgenic non-human animals, designing the construct should include as much flanking sequence of APOAV as to include all the regulatory elements that may be found in the flanking genomic DNA. One needs to consider the neighboring genes and whether or not they should be over-expressed as well. See Thomas, K. R. and Capecchi, M. R., Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51:503, 1987.

[0102]Thus in a specific embodiment, SEQ ID NO: 5, which is the 26 Kb XhoI isolated polynucleotide of the human APOAV region, can be used to create constructs that includes APOAV and APOAV flanking sequence but does not include neighboring APO genes. In a preferred embodiment, the targeting construct to delete mouse apoAV can be built using PCR products and primers made from SEQ ID NO: 7. For example, apoA5 knockout mice were generated by deleting the three exons predicted to encode apoA5 (FIG. 1A, FIG. 2).

[0103]In order to effect expression of the polynucleotides and polynucleotide constructs preferred embodiment, these constructs must be delivered to the host cell, where once it has been delivered to the cell, it may be stably integrated into the genome of the host cell and effectuate cellular expression. This delivery can be accomplished in vitro, for laboratory procedures for transforming cell lines, or in vivo or ex vivo, for the creation of therapies or treatments of diseases. Mechanisms of delivery include, but are not limited to, viral infection (where the expression construct is encapsulated in an infection viral particle), other non-viral methods known in the art such as, calcium phosphate precipitation, DEAE-dextran, electroporation, direct micro-injection, DNA-loaded liposomes, and receptor-mediated transfection of the expression construct. In a preferred embodiment, the delivery of the construct is by micro-injection into the appropriate host cell or by intravenous injection in the organism.

[0104]C. Correlation of APOAV Sequence Variants with Human Plasma Triglyceride Levels

[0105]Single nucleotide polymorphisms (SNPs) were first identified across and surrounding the human APOAV locus to serve as genetic markers for association. Six markers with relatively high minor allele frequencies (>8%) were obtained. Five of the SNPs were separated by three kbp within APOAV (SNP1-3, 5 and 6), while the fourth SNP (SNP4) was located ˜11 kbp upstream of the gene (FIG. 1A). These markers were scored in approximately 500 random unrelated normo-lipidemic Caucasian individuals who had been phenotyped for numerous lipid parameters before and after consumption of high- and low-fat diets.

[0106]Significant associations were found between both plasma triglyceride levels and VLDL mass and the five neighboring SNPs 1-3, 5 and 6 within APOAV but not with the distant upstream SNP4 (FIGS. 1A, 5A). Specifically, the minor allele of each of these SNPs (SNPs1-3, 5 and 6) was associated with higher triglyceride levels independent of diet. Independent analysis of each of SNPs 1-3, 5 and 6 revealed plasma triglyceride levels were 20-30% higher in individuals having one minor allele compared to individuals homozygous for the major allele (FIG. 4A). Two independent groups of individuals displayed increased triglycerides. First is a group of individuals with minor alleles at SNPs1-3, 6, while the second group of individuals contained the minor allele at SNP5.

[0107]Since the Caucasian population has two different apparent causative chromosomes for increased triglycerides and the allele frequency is ˜8% for both haplotypes, this observation effects a large number of individuals in the general population. (A minor allele frequency of 8% means there is an 8% probability for the rare haplotypes to occur on each chromosome.) Based on Hardy-Weinberg, the expected genotype distributions for such SNPs in the population can be calculated yielding 84.6% homozygous for the major allele, 14.7% heterozygous,

[0108]D. Transgenic Non-Human Animals to Assess Function of APOAV

[0109]The preferred embodiment also provides non-human animals to assess APOAV function. These non-human animals are preferably mammalian, even more preferably from the group consisting of mouse, rat, dog, chimpanzee, orangutan, baboon and macaque. These non-human animals are most preferably of the species Mus musculus, over-expressing human APOAV, as well as mice lacking apoA5 through standard mouse transgenic and gene knockout technologies (FIG. 2) (See K. A. Frazer, G. Narla, J. L. Zhang, E. M. Rubin, Nat Genet 9, 424-31 (1995) and C. Paszty, et al., Nat Genet 11, 33-39 (1995)). apoA5 knock-out animals and transgenic animals exhibit dramatic, but opposite effects on plasma triglyceride levels. apoA5 knockout animals exhibit a hyper-triglyceride phenotype, while the APOAV transgenic animals which over-express APOAV protein, exhibit a hypo-triglyceride phenotype.

[0110]APOAV transgenic animals, depending on the genetic background and the amount of overexpression, should exhibit at least two fold lower levels of plasma triglyceride. Multiple copies of the human APOAV gene result in an observed over-expression of the APOAV gene which can be determined by Northern blot analysis and result in reduced plasma triglyceride levels.

[0111]In addition to decreased triglyceride levels APOAV transgenic non-human animals should also have corresponding decreases in VLDL levels. This finding is consistent with the general knowledge that the majority of plasma triglyceride is carried on VLDL particles. VLDL levels can be characterized by fast protein liquid chromatography of lipoprotein particles from the animals or by other standard methods of lipoprotein determination such as ultracentrifugation.

[0112]An alternate embodiment also provides homozygous knockouts that are lacking apoA5 protein or lacking functional apoA5 protein. Transformed or transgenic cells, cell lines or non-human animals are obtained by homologous recombination of at least one apoA5 exon in embryonic stem cells, transfer of these stem cells to embryos, selection of the chimeras affected at the level of the reproductive lines, and growth of the said chimeras. Following successful germ-line transmission, heterozygous animals are then intercrossed.

[0113]The levels of very low-density lipoprotein (VLDL) particles increase in homozygous knockout animals and decrease in transgenic animals as compared with controls. Heterozygous knockout animals should exhibit VLDL levels intermediate between the homozygous knockout and control mouse. The peak VLDL elution volumes should remain similar in all animals, supporting comparable VLDL particle size, and that levels of other lipoproteins are not significantly altered.

[0114]To generate non-human animals which over-express APOAV, SEQ ID NO: 5, which is a 26.6 kbp XhoI human genomic DNA fragment predicted to contain only human APOAV, can be integrated into the genome of non-human embryos, thereby resulting in the expression of several copies of the human APOAV gene by the non-human animals. In addition, transgenic animals such as rats and rabbits, or transgenic continuous cell lines can be made. Furthermore, transgenic animals can be made using cDNA encoding human APOAV, both in its wild type and variants as described herein.

[0115]Transgenic non-human animals over-expressing the APOAV gene could be obtained by transfection of multiple copies of said APOAV gene under the control of a strong promoter of an ubiquitous nature, or promoters selective for a type of tissue, preferably liver tissue.

[0116]This embodiment also provides non-human animals for further animal studies by pharmaceutical companies to study APOAV. Animal studies that explore the regulation and expression of APOAV, its interaction with other apolipoproteins or other plasma, membrane or cellular proteins, production of antibodies for mutant and wild-type apoA5, and further in vivo study of apoA5. For example, mice lacking wild-type apoA5 may be exposed to various test substances to determine the triglyceride lowering effect of the test substance on individuals having a non-wild-type apoA5 gene. If a certain drug is no longer able to work, it would indicate that apoa5 is needed for the given drug to exert its affect.

[0117]Preferably, said transformed cells or mammals of the preferred embodiment will be used as a model allowing, in particular, the selection of products which make it possible to combat the pathologies induced by high levels of triglycerides.

[0118]In another embodiment, the non-human animals can be used to reveal the mechanism behind how apoA5 exerts its affect. Studies using the non-human animals can enable the elucidation of different mechanisms of triglyceride regulation, including but not limited to, clearance from the liver, secretion, production, catabolism and lipolysis of triglycerides. For example, to study clearance, one can identify a liver receptor or an alteration in the rate of VLDL clearance from the liver that apoA5 works through, which would prove to be a significant future target for drugs. The non-human animals may be used to show how apoA5 works in the liver to move triglycerides from the liver to the plasma, or if it is involved in increased lipolysis in the peripheries, or whether apoA5 has an effect on inflammation that leads to altered triglyceride levels.

[0119]E. Effects on Other Apolipoproteins in Transgenic Non-Human Animals

[0120]The observed changes in plasma triglyceride levels in apoA5 knockout and transgenic animals are directly opposite those previously reported in apoC3 knockout and transgenic mice (Y. Ito et al., Science 249, 790-3 (1990); N. Maeda, et al., J Biol Chem 269, 23610-6 (1994)). The apoA5 knockouts displayed an approximately 400% increase in plasma triglycerides compared to the 30% decrease noted in ApoC3 knockouts, while apoA5 transgenics showed decreased triglyceride levels compared to the increase reported in apoC3 transgenics.

[0121]The transgenic mice over-expressing human APOAV showed a decrease in apoCIII levels thereby suggesting a mechanism behind APOAV's effect on plasma triglyceride levels. Furthermore, mice lacking APOAV have increased apoCIII levels. Whether this direct association is coincidence or causal of the triglyceride phenotype remains to be determined.

[0122]Altered apoA5 expression affects apoC3 protein but not transcript levels in both apoA5 transgenic and knockout animals; apoC3 levels were increased ˜90% in apoA5 knockouts and decreased ˜40% in apoA5 transgenics. These data suggest that apoC3 may exert its effect on triglyceride levels by altering apoA5 levels.

[0123]Because alterations in apoA5 expression lead to changes in apoC3 protein levels, the effect on triglycerides may be mediated through apoC3. The fact that apoA5 transgenic mice have two-fold lower triglycerides than the previously described apoC3 knockout mice indicate that changes in apoC3 alone can not explain the entire effect of apoA5. In addition to APOC3, the over-expression of several human apolipoprotein transgenes has been shown to increase triglyceride levels in mice, while only the APOAV transgene leads to decreased triglycerides suggesting a novel mechanism behind this effect.

[0124]While not being bound to one theory, the inventors theorize that the APOAV gene product (protein) interacts with other proteins in the apo family (e.g. APOC3) in such a way as to affect their levels, and thereby triglyceride levels. The inventors describe a direct correlation between APOAV and APOC3. Thus, this embodiment also provides non-human animals to explore the regulation and expression of apoA5 and apoC3, the interaction between these two apolipoproteins and other apolipoproteins, and further in vivo study of APOC3. APOC3 is known to inhibit triglyceride lipolysis on VLDL, thus contributing to higher levels of plasma triglyceride and VLDL. The transgenic mice over-expressing human APOAV showed a decrease in APOC3 levels thereby suggesting a mechanism behind APOAV's effect on plasma triglyceride levels. Furthermore, mice lacking APOAV have increased APOC3 levels.

[0125]Therefore, the preferred embodiment includes a method for determining predisposition towards elevated triglyceride levels of an individual, comprising determining the level of APOAV gene expression, wherein elevated APOAV gene expression is associated with decreased elevated triglycerides and lowered APOAV gene expression is associated with increased elevated triglycerides. The method further comprising determining the level of APOC3 gene expression, wherein lowered APOC3 gene expression is associated with decreased elevated triglycerides and elevated APOC3 gene expression is associated with increased elevated triglycerides.

[0126]F. APOAV Haplotypes and Frequencies

[0127]The population frequency for each haplotype is the percentage of individuals who have a given haplotype. Statistically, approximately 50-75% of the population is homozygous for the common haplotype (*1/*1) that is correlated with lower triglyceride levels, while approximately 25-50% of the population contains at least one copy of the minor haplotypes (APOA5*2 and/or APOA5*3) which is correlated with increased triglyceride levels. In addition, approximately 0.6-1.5% of the population is homozygous with both chromosomes containing the rare haplotypes (*2/*2, *3/*3 or *2/*3), which is correlated with the highest triglyceride levels.

[0128]Association studies that were conducted indicate the existence of three haplotypes in APOAV present in the human population, which are associated with plasma triglyceride levels. Preliminary studies in this population found no significant association of triglyceride levels with the Sst1 polymorphism in APOC3 (located ˜40 kbp upstream of APOAV) (FIG. 1A) which has been previously associated with severe hyper-triglyceridemia (M. R. Hayden, et al., Am J Hum Genet 40, 421-30 (1987); M. Dammerman, L. A. Sandkuijl, J. L. Halaas, W. Chung, J. L. Breslow, Proc Natl Acad Sci USA 90, 4562-6 (1993). This finding suggests the APOC3 Sst1 polymorphism is not a marker for the metabolic effect defined by the APOAV haplotypes.

[0129]The three haplotypes (APOA5*1, APOA5*2, APOA5*3) are composed of biallelic markers at the following positions on APOAV: -1131T>C (SNP3), c.-3A>G (SNP6), c.56C>G (SNP5), IVS3+476G>A (SNP2) and c.1259>C (SNP1). Table 2 shows the three haplotypes and the relative frequencies that each appears in the Caucasian general population.

TABLE-US-00002 TABLE 2 -1131T > C c.-3A > G c.56C > G IVS3 + 476G > A c.1259T > C Haplotype Frequency (SNP3) (SNP6) (SNP5) (SNP2) (SNP1) APOA5*1 81.6% T A C G T APOA5*2 8.0% C G C A C APOA5*3 8.0% T A G G T

[0130]The frequency listed for each haplotype is the relative frequency per chromosome, meaning that statistically, approximately 75% of the Caucasian population is homozygous for the common haplotype (*1/*1) that is correlated to low triglyceride levels, approximately 25% of the Caucasian population is heterozygous with one chromosome having the common haplotype and the other containing a rare haplotype (APOA5*2 or APOA5*3) which is correlated to raised triglyceride levels and approximately 0.6% or less than 1 percent of the population is homozygous with both chromosomes containing the rare haplotypes (*2/*2, *3/*3 or *2/*3), which correlates to the highest triglyceride levels. In addition to APOAV's strong association with triglyceride levels in Caucasians, a strong effect is also seen African-Americans and Hispanics where the minor allele frequencies are higher. Thus, a larger percent of African-Americans and Hispanics display increased triglycerides due to the genetic effect of APOAV. Specifically, APOA5*2 and/or APOA5*3 is present in 36% of African Americans and 51% of Hispanics and results in an ˜25% increase in triglycerides compared to APOA5*1 homozygotes.

[0131]Thus, the preferred embodiment includes a method of determining an individual's total risk of lipid-related diseases or disorders by identifying an individual's APOAV haplotype on each chromosome. One needs only genotype individuals at two different polymorphic loci, wherein one of those loci is SNP5, to determine which haplotypes the individual possesses, and whether the individual is heterozygous or homozygous for the rare or normal alleles (defining APOA5*3). The haplotypes can be easily determined by detecting the genotype of individuals at SNPs 1-3 or 6 (APOA5*2) and at SNP5 (APOA5*3) for both copies of chromosome 11. Based on the knowledge of what haplotypes the individual possesses, the amount of risk for lipid-related diseases or disorders can then be determined or predicted. For example, if the individual is genotyped and found to have a T at SNP3 on one chromosome and a C at SNP3 on the other chromosome, then it can be determined that the individual is heterozygous, having APOA5*2 haplotype on one of the chromosomes. Then genotyping the individual at SNP5 will distinguish whether the other chromosome is a rare haplotype (APOA5*3) or the normal haplotype (APOA5*1). Methods of detecting SNPs and genotyping are discussed in the Diagnostic Applications section.

[0132]G. Diagnostic Applications

[0133]The present embodiment enables genetic testing for APOAV and its correlation to increased triglyceride levels in people having polymorphisms deviating from the normal or "wild type" phenotype. Further, a combination test with APOC3 is suggested. Genetic testing may be carried out on a patient's DNA or RNA or protein, provided that antibodies capable of distinguishing mutant from wild type APOAV protein are available.

[0134]1. Antibodies to APOAV and its Variants

[0135]Antibodies including both polyclonal and monoclonal antibodies, and drugs that modulate the production of activity of APOAV possess certain diagnostic applications and may, for example, be utilized for the purpose of detecting the identity of the haplotype of individuals. For example, wild type APOAV and its variants may be used to produce both polyclonal and monoclonal antibodies in a variety of cellular media, by known techniques such as the hybridoma technique utilizing, for example, fused mouse spleen lymphocytes and myeloma cells. Likewise small molecules that mimic or agonize the activity(ies) of APOAV may be discovered or synthesized, and may be used in diagnostic and/or therapeutic protocols.

[0136]The general methodology for making monoclonal antibodies by hybridomas is well known. Immortal, antibody-producing cell lines can be created by techniques other than fusion, such as direct transformation of B lymphocytes with oncogenic DNA, or transfection with Epstein-Barr virus. See, e.g., M. Schreier et al., "Hybridoma Techniques" (1980); Hammerling et al., "Monoclonal Antibodies And T-cell Hybridomas" (1981); Kennett et al., "Monoclonal Antibodies" (1980); see also U.S. Pat. Nos. 4,341,761; 4,399,121; 4,427,783; 4,444,887; 4,451,570; 4,466,917; 4,472,500; 4,491,632; 4,493,890.

[0137]Panels of monoclonal antibodies produced against APOAV peptides can be screened for various properties; i.e., isotype, epitope, affinity, etc. Of particular interest are monoclonal antibodies that specifically bind and identify the alleles of APOAV, and can distinguish between the rare and the normal alleles of APOAV. In one preferred embodiment, a monoclonal antibody can be generated that specifically binds to the W19 position of the APOAV protein, which results from the rare SNP5 allele. Such monoclonals can be readily identified in, for example, gel-shift assays. High affinity antibodies are also useful when immunoaffinity purification of native or recombinant APOAV is possible.

[0138]A preferred method of generating these APOAV allele-specific antibodies is by first synthesizing peptide fragments. These peptide fragments should cover at least SNP5 and the adjacent amino acid sequence. Subsequent antibodies should be screened for their ability to distinguish the two protein variants. Since synthesized peptides are not always immunogenic on their own, the APOAV peptides should be conjugated to a carrier protein before use. Appropriate carrier proteins include but are not limited to Keyhole limpet hemacyanin (KLH). The conjugated peptides should then be mixed with adjuvant and injected into a mammal, preferably a rabbit through intradermal injection, to elicit an immunogenic response. Samples of serum can be collected and tested by ELISA assay to determine the titer of the antibodies and then harvested.

[0139]Polyclonal APOAV allele-specific antibodies can be purified by passing the harvested antibodies through an affinity column. Monoclonal antibodies are preferred over polyclonal antibodies and can be generated according to standard methods known in the art of creating an immortal cell line which expresses the antibody.

[0140]Additionally, spleen cells can be harvested from the immunized animal (typically rat or mouse) and fused to myeloma cells to produce a bank of monoclonal antibody-secreting hybridoma cells. The bank of hybridomas can be screened for clones that secrete immunoglobulins that bind the protein of interest specifically, i.e., with an affinity of at least 1×10⁷ M^-1. Animals other than mice and rats may be used to raise antibodies; for example, goats, rabbits, sheep, and chickens may also be employed to raise antibodies reactive with an APOAV protein. Transgenic mice having the capacity to produce substantially human antibodies also may be immunized and used for a source of antiserum and/or for making monoclonal antibody secreting hybridomas.

[0141]Bacteriophage antibody display libraries may also be screened for phage able to bind peptides and proteins specifically. Combinatorial libraries of antibodies have been generated in bacteriophage lambda expression systems and may be screened as bacteriophage plaques or as colonies of lysogens. For general methods to prepare antibodies, see Antibodies: A Laboratory Manual (1988), E. Harlow and D. Lane, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., incorporated herein by reference.

[0142]These antibodies can in turn be used to isolate APOAV proteins from normal or recombinant cells and so can be used to purify the proteins as well as other proteins associated therewith. Such antibodies are useful in the detection of specific alleles of APOAV proteins in samples and in the detection of cells comprising APOAV proteins in complex mixtures of cells. Such detection methods have application in screening, diagnosing, and monitoring lipid metabolism related diseases and other conditions, such as high levels of triglycerides.

[0143]2. Genotyping and Haplotyping

[0144]Any method known in the art can be used to identify the nucleotide present at one of the disclosed APOA5 polymorphic sites. Since the SNPs and haplotypes to be detected have been identified and specified in the present invention, detection will prove simple for one of ordinary skill in the art. Any number of techniques to detect the haplotype of an individual by genotyping the individual at certain polymorphic sites can be used, including, but not limited to, the following.

[0145]The nucleotide can be determined by sequencing analysis after DNA samples are subjected to PCR amplification. Preferably, the amplified DNA is subjected to automated dideoxy terminator sequencing reactions using a dye-primer cycle sequencing protocol. The sequencing reactions are then sequenced using any number of commercially available sequencing machines such as the ABI 377 or 3700 Sequence Analyzer (Applied Biosystems, Foster City, Calif.).

[0146]Techniques and methods of synthesizing and amplifying polynucleotides by litigation of multiple oligomers (LMO) onto a template-bound primer are also described by Akhavan-Tafti in U.S. Pat. Nos. 5,998,175; 6,001,614; 6,013,456; and 6,020,138, which are hereby incorporated by reference in their entirety. Short polynucleotides, 5 to 10 bases long, can be supplied as a library of oligonucleotides and are simultaneously ligated, using a suitable ligase enzyme, to a template-bound primer in a contiguous manner to produce a complementary strand of template polynucleotide. If the sequence to be synthesized is known, a set containing the minimum number of oligomers can be used and are then ligated by DNA Ligase in the correct order starting from the primer, uni- or bi-directionally, to produce the complementary strand of a single-stranded template sequence.

[0147]A preferred method is to use sequence detection/amplification assays such as the INVADER assays which are commercially available from Third Wave Technologies (Madison, Wis.) to genotype samples. Such systems rely on an enzyme-substrate reaction to amplify signal generated when a perfect match with an (rare) allele of APOAV is detected. See Dahlberg, J. et al., U.S. Pat. Nos. 5,846,717 and 5,888,780, which are hereby incorporated by reference in their entity.

[0148]A third preferred method is using methods that have been developed for examining single base changes without direct sequencing. For example, if a mutation of interest happens to fall within a restriction recognition sequence, a change in the pattern of digestion can be used as a diagnostic tool (e.g., restriction fragment length polymorphism [RFLP] analysis) See U.S. Pat. Nos. 5,547,835; 6,221,601; 6,194,144 which are hereby incorporated by reference in their entirety. Other methods of SNP analysis are performed by companies such as Sequenom (San Diego, Calif.), which can genotype many samples very quickly and with great accuracy, non-sequencing methods such as MALDI-TOF, miniaturized chip-based array formats and mass spectrometry.

[0149]Other genotyping methods suited for detection of SNPs include, but are in no way limited to, LCR (ligase chain reaction), Gap LCR (GLCR), using allele-specific primers, mismatch detection assays, microsequencing assays, and hybridization assay methods.

[0150]3. Oligonucleotide Primers and Probes

[0151]Various methods for screening for genetic APOAV abnormalities in individuals can be employed. Polynucleotides according to SEQ ID NOs: 1-3 and 5-7 can also be used in gene marker assays, probes, primers for uses including, but not limited to, PCR, sequencing, hybridization assays and probes. Variations that are at least 95% or more homologous to the sequences of APOAV polynucleotides (e.g. SEQ ID NOs: 1-3, 5 and 6) may also be used for comparison studies and in any of the above listed types of assays. In another embodiment, polypeptides that are at least 95% of more homologous to APOAV protein (e.g. SEQ ID NO: 4) or to the protein generated from polynucleotide sequences selected from the group consisting of SEQ ID NO: 1-3, 5-6 may be used for lipid studies.

[0152]The preferred embodiment also encompasses APOAV oligonucleotide primers made from SEQ ID NO: 3 and 4 and capable of amplifying the DNA sequence of and surrounding each of SNPs 1-6. Basic primer design considerations such as annealing/melting temperature, length, repetitive DNA, proximity to the SNP, and specificity will be appreciated and addressed by one skilled in the art. Many programs that enable one to pick and design custom primers address these considerations, such as PRIMER3 (S. Rozen and H. J. Skaletsky).

[0153]Suitable primers such as those disclosed in Table 3, SEQ ID NOs: 8-36 and primers made from sequence up to about 500 base pairs away from the SNP can be used for amplification, may be used to assay SNP5 (position 12974). and/or sequencing these SNPs. For example, SEQ ID NO: 24 (AV1-F 5' TGCTCACCTGGGCTCTGGCTCTTC) and SEQ ID NO: 25 (AV1-R 5' CCAGAAGCCTTTCCGTGCCTGGGCGGC) which lie in SEQ ID NO: 3 and 4 at positions 12824-12847 and 12976-13002 respectively Furthermore, depending upon the genotyping strategy used, other probes and primers can be designed from SEQ ID NOs: 1-6 for use in such assays as PCR-RFLP and PCR INVADER assays (Third Wave Technologies, Madison, Wis.).

[0154]The following table shows primers that can be used to amplify genomic DNA surrounding SNPs 1-6 by such methods as PCR. The examples of forward and reverse primers that can also be used to amplify sequence containing each of the described SNPs 1-6. The resulting amplified product can be genotyped by methods including, but not limited to, sequencing, mass spectrometry, RFLP, and INVADER assays. Primers such as those disclosed in Table 3, SEQ ID NOS: 8-36 and primers made from sequence up to about 500 base pairs away from the SNP can be used for amplification and/or sequencing these SNPs. For example, SEQ ID NO: 24 (AV1-F 5' TGCTCACCTGGGCTCTGGCTCTTC) and SEQ ID NO: 25 (AV1-R 5' CCAGAAGCCTTTCCGTGCCTGGGCGGC

TABLE-US-00003 TABLE 3 SEQ Length of ApoA5 ID Position on amplified SNP NO Primer Sequence ApoA5 gene sequence ApoA5- 39 ATGACCTGTGGGAAGACATCACT Forward 14470-14492 455 bp SNP1 40 AGCCAGAAGTGACTAGAGCCAAA Reverse 14902-14924 ApoA5- 41 AGTCCCCAGAATCAAAGGATGAT Forward 13341-13363 497 bp SNP2 42 ATCGTGTAGGGCTTCAGTTGCT Reverse 13816-13837 ApoA5- 43 CCTGTCTTCTCAGAGCAGGTAATG Forward 12784-12807 285 bp SNP5 44 AGCCATCTTCTGCTGATGGATCT Reverse 13046-13068 ApoA5- 45 AAGACACCCTAGCCTCCTTGACT Forward 12602-12624 566 bp SNP3 + 46 ACAGAGGTTGAGGCAGCAGAG Reverse 13147-13167 SNP6 ApoA5- 47 GTAGTGAAAATCAGGGGCCTTCT Forward 484-506 158 bp SNP4 48 ATGCATAAACCCAAAGGGAAAAT Reverse 619-641

[0155]In one embodiment of the invention, fragments of various lengths of APOAV DNA may be placed onto solid supports for use in gene chips or other parallel formats for assay purposes. In general, these methods employ arrays of oligonucleotide probes that are complementary to targeted nucleic acid sequences, and allow for detection when the sample hybridizes to a probe on the array. In preferred embodiments, the nucleic acid sequences are APOAV fragments of about 15-30 nucleotides in length, specifically sequences containing APOAV SNPs 1-6. In further embodiments, the chip may comprise an array including at least one of the sequences selected from the group consisting of amplification primers listed in Tables 3 and 4. See D. J. Lockhart, et al., "Expression monitoring by hybridization to high-density oligonucleotide arrays," Nature Biotechnology, 14:1675-1680, December 1996, for useful methods and heuristics in designing oligonucleotide probes from APOAV fragments.

[0156]Chips of various formats from companies such as Agilent Technologies (Palo Alto, Calif.) and Affymetrix (Santa Clara, Calif.) can be produced on a customized basis by various methods. Alternatively, DNA microarray chips are fairly inexpensive to make and assemble. Individual samples to be tested are then contacted with the oligonucleotide probes and the genotype and/or haplotype of the sample can be determined based on detection of the hybridization between the probes and the sample. A suitable DNA micro-array is disclosed in Brown et al. U.S. Pat. No. 5,807,522

[0157]H. Modulating and Regulating, APOAV Expression

[0158]The preferred embodiment also encompasses methods of modulating and regulating APOAV expression. Current therapies known to effect lipid metabolism can also be studied for their effect in modulating and regulating APOAV expression. Current methods include but are not limited to, administration of fibrates and other molecules important in inflammatory response, cholesterol regulating drugs, and glucose and insulin regulatory molecules.

[0159]For example, fibrates are hypolipidemic drugs with pleiotropic effects on lipid metabolism including the reduction of plasma triglycerides. Suitable fibrate drugs are disclosed in U.S. Pat. No. 4,318,923 issued to Hamayaki et al. And hereby incorporated by reference. Classically, the triglyceride lowering action of fibrates is explained by decreased hepatic secretion of VLDL and an enhancement in plasma triglyceride clearance. Several studies established that this effect is mediated through the induction of lipoprotein lipase expression and down-regulation of APOC3 expression by fibrates. A major means by which fibrates regulate the expression of lipid metabolism-related genes by fibrates has been shown to be via activation of the peroxisome proliferator-activated receptor alpha (PPARα). Three distinct PPARs (α, β, and γ) have been described in different species. Whereas PPARβ appears ubiquitously expressed, PPARα and PPARγ are mainly expressed in liver and in adipose tissue, respectively. PPARs are ligand-activated nuclear receptors that dimerize with the retinoid X receptor (RXR) and bind to specific DNA sequence defined as peroxisome proliferator response elements (PPRE). Upon binding, PPARs activate gene transcription.

[0160]Given the determinant link between APOAV and plasma triglycerides and the widespread use of fibrates in the treatment of dyslipidemia, one could investigate how fibrates affect APOAV gene expression and consequently influence plasma triglyceride levels. It is very likely that studies in mice and in vitro studies with human hepatocytes reveal that fibrates dramatically increase APOAV expression.

[0161]To determine if fibrates effect on apoa5 is mediated via the PPARα pathway, sequence conservation comparison, in vitro promoter analyses and functional studies of putative PPREs to the APOAV gene can be performed. These and other studies may identify fibrates acting via PPARα as a crucial regulator of the new apolipoprotein APOAV and suggest a novel and likely clinically relevant mechanism of how PPARα activators can act on lipid homeostasis. Modulation of APOAV via a PPARα pathway would prove to offer a new target for therapeutic interventions designed at correcting hypertriglyceridemia and at limiting triglyceride-associated cardiovascular risk.

A. I. Drug Design and Therapies Based on Sequence Variations

B. 1. Drug Screening and Design

[0162]In addition to modulating the expression of the APOAV gene, the present embodiment further contemplates an alternative method for identifying specific agonists and activators using various screening assays known in the art.

[0163]The preferred embodiment contemplates screens for small molecule ligands or ligand analogs and mimics, as well as screens for natural ligands that bind to and agonize APOAV activity in vivo or result in lowered levels of triglycerides. For example, natural products libraries can be screened using assays of the invention for molecules that agonize APOAV activity. Knowledge of the primary sequence of the various APOAV allele variants and other structural motifs of APOAV (i.e., amphipathic α-helices), and the similarity of those sequences with domains contained in other proteins, can provide an initial clue to agonists of the protein. Identification and screening of agonists is further facilitated by determining structural features of the protein, e.g., using X-ray crystallography, neutron diffraction, nuclear magnetic resonance spectrometry, and other techniques for structure determination. These techniques provide for the rational design or identification of agonists of APOAV that will reduce triglyceride levels.

[0164]Another approach uses recombinant bacteriophage to produce large libraries. Using the "phage method" Scott and Smith, 1990, Science 249: 386-390 (1990); Cwirla, et al., Proc. Nat. Acad. Sci., 87: 6378-6382 (1990); Devlin et al., Science, 249: 404-406 (1990), very large libraries can be constructed. A second approach uses primarily chemical methods, of which the Geysen method, Geysen et al., Molecular Immunology 23: 709-715 (1986); Geysen et al. J. Immunologic Method 102: 259-274 (1987), and the method of Fodor et al. Science 251: 767-773 (1991) are examples. Houghton in U.S. Pat. No. 4,631,211, and Rutter et al., U.S. Pat. No. 5,010,175, describe methods to produce a mixture of peptides that can be tested as agonists or antagonists.

[0165]In another aspect, synthetic libraries and the like can be used to screen for ligands that recognize and specifically bind to APOAV and its variants. In one such example, a phage library can be employed. Phage libraries have been constructed which when infected into host E. coli produce random peptide sequences of approximately 10 to 15 amino acids, Parmley and Smith, Gene, 73: 305-318 (1988), Scott and Smith, Science, 249: 386-249 (1990). Specifically, the phage library can be mixed in low dilutions with permissive E. coli in low melting point LB agar which is then poured on top of LB agar plates. After incubating the plates at 37° C. for a period of time, small clear plaques in a lawn of E. coli will form which represents active phage growth and lysis of the E. coli. A representative of these phages can be absorbed to nylon filters by placing dry filters onto the agar plates. The filters can be marked for orientation, removed, and placed in washing solutions to block any remaining absorbent sites. The filters can then be placed in a solution containing, for example, a radioactive fragment of APOAV. After a specified incubation period, the filters can be thoroughly washed and developed for autoradiography.

[0166]Plaques containing the phage that bind to the radioactive binding domain can then be identified. These phages can be further cloned and then retested for the ability to bind to APOAV and/or its variants. Once the phages have been purified, the binding sequence contained within the phage can be determined by standard DNA sequencing techniques. Once the DNA sequence is known, synthetic peptides can be generated which represent these sequences.

[0167]The effective peptide(s) can be synthesized in large quantities for use in in vivo models and eventually in humans to reduce triglyceride levels. Synthetic peptide production is relatively non-labor intensive, easily manufactured, quality controlled and thus, large quantities of the desired product can be produced quite cheaply. Similar combinations of mass produced synthetic peptides have recently been used with great success. Patarroyo, Vaccine, 10: 175-178 (1990). The peptides may be prepared according to known pharmaceutical technology. They may be administered singly or in combination, and may further be administered in combination with other cardiovascular drugs. They may be conventionally prepared with excipients and stabilizers in sterilized, lyophilized powdered form for injection, or prepared with stabilizers and peptidase inhibitors of oral and gastrointestinal metabolism for oral administration.

[0168]Another embodiment is to create a cell system which has the 5' regulatory region of the human APOAV gene coupled to a reporter gene, such as luciferase, as is known in the art. The luciferase gene is positioned at the start of the APOAV gene. Candidate drugs are screened against the cell system and scored for their ability to upregulate the luciferase expression. These drugs will have use in lowering plasma triglycerides, according to the findings of the inventors that increased levels of the APOAV protein cause lowered plasma triglycerides as shown by Example 3.

[0169]Other high-throughput methods of drug design and discovery are discussed in Landro, J. A. et al., "HTS in the new millennium, the role of pharmacology and flexibility," J Pharmacol Toxicol Methods, 2000 July-August; 44(1):273-89, describing target identification, reagent preparation, compound management, assay development, high-throughput library screening and other methods for drug discovery and screening, and is hereby incorporated by reference in its entirety.

[0170]While lowering triglyceride levels is an aim of the preferred embodiment, other embodiments target other metabolite levels such as insulin or glucose levels, by modulating APOAV gene expression. As show in Example 9, APOAV levels can lead to changes in plasma glucose or insulin levels or other metabolite levels. Therefore, alternate embodiments contemplate the aforementioned methods of drug screening and drug design for the purpose of modulating APOAV to affect other metabolite levels.

[0171]2. Gene Therapy with APOAV

[0172]The preferred embodiment also encompasses uses of the APOAV gene for gene therapeutics such as those described by Gabor M. Rubanyi, "The future of gene therapy," Molecular Aspects of Medicine 22(2001): 113-142, and is hereby incorporated by reference in its entirety. Rubanyi describes existing and future methods of gene therapy and the technical hurdles gene therapy faces in the future are made possible through the sequences disclosed in SEQ ID NO: 1-7. Other examples are drug therapies aimed at raising the levels of APOAV in any human patient with high triglyceride levels. These will provide a suitable way to reduce triglyceride levels and thereby reduce the risk of cardiovascular disease. Further aims include determining how APOAV exerts its effect upon triglyceride and other metabolite levels and to stimulate that pathway by non-APOAV means as a way to lower triglycerides or modulate other metabolite levels.

[0173]As described in an earlier section, various types of gene delivery vectors can be used including, but definitely not limited to, plasmids, YACs (Yeast Artificial Chromosomes), BACs (Bacterial Artificial Chromosomes), bacterial vectors, bacteriophage vectors, viral vectors (for example, retroviruses, adenoviruses and viruses commonly used for gene therapy), non-viral synthetic vectors, and recombinant vectors. Delivery of the vector and/or construct for gene therapy in a preferred embodiment is by viral infection or injection intravenously although delivery can be by any other means as described previously.

[0174]A preferred embodiment is modelled after the method described by Tangirala R K et al., Circulation, 1999 Oct. 26; 100(17):1816-22, wherein the regression of atherosclerosis was induced by liver-directed gene transfer of apolipoprotein A-I in mice. The preferred embodiment contemplates a similar protocol of gene transfer as Tangirala et al. based on the same target tissue and the desire to express APOAV endogenously in the liver. A second-generation recombinant adenovirus encoding SEQ ID NO: 1 or 2, human APOAV cDNA can be constructed as described by Tsukamoto K. et al., Journal of Lipid Research, 1997:38, 1869-1876. Briefly, pAdCMV APOAV can be linearized with an enzyme and co-transfected into cells along with adenoviral DNA isolated and digested. The cells are then overlaid with agar and incubated at 32° C. for about 15 days. Plaques positive for APOAV cDNA are subjected to a second round of plaque purification, and the recombinant adenovirus is then expanded in cells at 32° C. A null adenovirus can be constructed and expanded in an identical manner. All viruses are then purified and stored appropriately.

[0175]While much of gene therapy uses vectors as a means of delivery, other methods of delivery to the somatic cells of a patient may be utilized. The preferred embodiment also contemplates the delivery of APOAV polynucleotides by encapsulation by compositions such as, hydrogels and microgels, liposomes, and other lipid or polymer carriers. Furthermore, the APOAV polynucleotides can be delivered naked, without any means of receptor-mediated entry or other carrier into the patient's cells.

[0176]3. Therapeutics Using APOAV

[0177]The presently disclosed APOAV polynucleotides and polypeptides, and fragments thereof, may be prepared according to known pharmaceutical technology. They may be administered singly or in combination, and may further be administered in combination with other cardiovascular or triglyceride-lowering drugs. They may be conventionally prepared with excipients and stabilizers in sterilized, lyophilized powdered form for injection, or prepared with stabilizers and peptidase inhibitors of oral and gastrointestinal metabolism for oral administration. They may also be administered by methods including, but not limited to, intravenous, infusion, rectal, inhalation, transmuscosal or intramuscular administration.

[0178]The APOAV polynucleotides and polypeptides can be isolated, recombinant or synthesized, so long as the polynucleotides and polypeptides maintain APOAV functionality. In a preferred embodiment, the APOAV polynucleotide of SEQ ID NO: 1-3 or 5 is delivered in the therapy whereby the APOAV gene is over expressed in the organism. In other preferred embodiments, the polypeptide or active APOAV protein of SEQ ID NO: 4 is delivered to lower triglyceride levels.

[0179]Combining data from stratification and genetic studies with diagnostic tests to determine the best method of treatment for person based upon such criteria as specific haplotype, age, gender and ethnicity. For example, after finding in a genetic study that individuals having haplotypes APOA5*1/*2 and a specified triglyceride level, respond to a certain dosage of fibrates (e.g. their triglyceride levels dramatically are reduced by an average 50 dl/mL), physicians and medical providers can tailor triglyceride therapy to prescribe the most effect dosage of triglyceride lowering medication. After ordering the diagnostic tests described earlier for individuals to determine what haplotype they possess, doctors and other medical providers can then prescribe the most effective dosage to achieve the goal of dramatically reducing triglycerides to persons having the haplotypes of APOA5*1/*2. In other embodiments, the sample of individuals can be broken down according to other criteria, including, but not limited to, age, gender, ethnicity, diet or the presence or absence of certain disease symptoms.

[0180]J. Methods of Genetic Analysis and Association Studies

[0181]In general, the SNPs of this invention find use in any method known in the art to demonstrate a statistically significant correlation between a genotype and phenotype, and between a haplotype and phenotype. Preferably, the SNPs are used in studies to determine their correlation to lipid metabolism disorders. More preferably, the SNPs are used in studies to determine whether they are causative mutations of lipid metabolism disorders.

[0182]The described polymorphisms can be used to separate individuals based on any phenotypic trait. For instance, patients can be treated with fibrates and their triglyceride levels can be determined. Individuals can then be separated based on their APOAV genotype/haplotype (APOA5*1, APOA5*2, or APOA5*3) and their average triglyceride level determined. This will enable a physician to address if APOAV polymorphisms influence how responsive an individual will be to a triglyceride therapy.

[0183]A similar strategy could be used for any drug therapy. As another example, a certain diseased group of individuals could be separated based on their APOAV genotype/halotype, and all the average phenotypes from these groups can be examined for differences. If any phenotype display shows a difference, this would be a phenotype that APOAV may influence. For instance, a group of diabetics could be separated based on their APOAV genotype. Numerous phenotypes in these subgroups can be averaged and compared, such as glucose levels. If there is a difference in glucose levels, this would support the proposal that APOAV influences glucose levels in diabetes. Another example would be to look at every type of cardiovascular disease and see if there is an increased frequency of the minor haplotypes in the diseased group compared to controls. If there is a difference then APOAV likely contributes to this disease.

[0184]Criteria or methods for selecting individuals for treatments, drug trials and any of the studies described herein include, but are not limited to, such criteria for eligibility as: willingness to participate in program, no medication use likely to interfere with lipid metabolism, percentage of ideal body weights according to such tables and indices available such as Metropolitan Life Insurance Company Tables (1985), certain body mass index, free of chronic disease, nonsmoker, daily alcohol consumption, related or unrelated to other subjects in the study, family and other relatives living and willing to donate blood samples or submit to studies, belonging to certain age and/or ethnicity groups, possessing defined levels of plasma total cholesterol, triacylglycerols and blood pressure, adherence to diet and/or exercise protocol and requirements, and any other measurable genotypic or phenotypic trait. In addition to meeting this criteria, analysis of the plasma lipids, lipoproteins, lipoprotein subfractions; triglycerides and apolipoproteins of the subjects should be done to develop complete profiles of each subject.

[0185]For more examples of preferred subject criteria and methods of measuring triglycerides, lipoproteins, cholesterol, and other related lipid metabolism proteins and methods for conducting clinical trials as herein described, see D. Dreon et al., Arteriosclerosis, Thrombosis, and Vascular Biology. 1997; 17:707-714; D. Dreon et al., Am J Clin Nutr. 1998; 67:828-36; and Williams et al., Arteriosclerosis, Thrombosis, and Vascular Biology. 1997; 17:702-706, which are hereby incorporated by reference in their entirety.

[0186]The preferred embodiment permits genetic analysis studies between the disclosed SNPs 1-6, the APOAV haplotypes (APOA5*1/*2/*3) and any phenotype. In general, the SNPs and haplotypes of the present invention find use in any method known in the art to demonstrate a statistically significant correlation between a genotype and phenotype. The genetic analysis using the SNPs and haplotypes that may be conducted include but are not limited to linkage analysis, population association studies, allele frequencies, haplotype frequencies, and linkage disequilibrium.

[0187]Linkage analysis is based upon establishing a correlation between the transmission of genetic markers and that of a specific trait throughout generation within a family. Thus, the aim of linkage analysis is to detect marker loci that show co-segregation with a trait of interest. Linkage analysis correlating APOAV SNPs and haplotypes and the trait of high triglyceride levels within families or people/ethnic groups are an aim of this invention. The examples demonstrate linkage analysis studies that correlated the presence of either APOA5*2 or APOA5*3 with raised triglyceride levels in the Caucasian, African-American and Hispanic populations. Further linkage analysis is also contemplated for studies of other people and ethnic groups, and further regional studies including groups in other countries. Linkage analysis can be performed according to parametric or non-parametric methods.

[0188]Frequency of alleles and haplotypes in a population is also another genetic analysis study contemplated by the invention. Using the genotyping and haplotyping methods described in the earlier "Genotyping and Haplotyping" section, one skilled in the art can determine the frequency of SNPs 1-6 and haplotypes APOA5*1/*2/*3 in a given population. While several methods of estimating allele frequency are possible, genotyping individual samples is preferred over genotyping pooled samples due to higher sensitivity, reproducibility and accuracy. Furthermore, many genomic and large-scale sequencing centers enable rapid genotyping and haplotyping by sequencing methods and thereby provide rapid data production.

[0189]Association studies between APOAV SNPs and haplotypes and any phenotype can also be performed on a random sample of people, anywhere from a few hundred to tens of thousands. After collecting various parameters for each individual participating the study, such as height, weight, triglyceride levels, medical history, etc., the sample group can be separated according to various genotypes at APOAV. Any repeated differences in the parameters in individuals that are observed are likely traits that are associated with one of the APOAV genotypes or haplotypes. Examples show that there are differences in triglyceride levels that are associated with APOAV haplotypes *1, *2 and *3, however, there are likely other associations that can be subject to study. Other parameters to observe include, but are not limited, presence of cardiovascular disease risks, other lipid, lipoprotein or protein levels, instances of diabetes, obesity, inflammatory diseases, inflammatory response, apolipoprotein expression levels, alcoholism and drug abuse.

[0190]Alternate embodiments also encompass a method of determining if SNPs 1-6 are in linkage disequilibrium with any lipid-related or other disorders.

[0191]Studies correlating the genotype/haplotype with methods and treatments of high triglycerides or other lipid-related disorder are also contemplated. Segregation of individuals in the study according to their response (e.g. lowering of triglyceride levels) to various drug therapies and combinations and then according to the APOAV allele frequency. The result of stratification of population studies would enable doctors and medical care providers to prescribe therapy with greater accuracy, and with greater success rates. Thus, therapy prescribed would be "tailor-made" for individuals based upon their haplotypes.

[0192]Statistical methods and computer programs useful for linkage analysis, genetic analysis and association studies are well-known to those skilled in the art. Any statistical tool useful to test for statistically significant associations between genotypes, haplotypes and phenotypes, comparisons and correlations between a biological marker and any physical trait, and frequency comparisons may be used.

[0193]Statistical analyses can be carried out using the SAS computer program (SAS, Cary, N.C.) and similar programs. Plasma triglyceride concentrations can be compared among different genotype groups using Wilcoxon's test and the like. Allele frequencies should be compared using such tests as Fisher's exact test. To determine pairwise linkage disequilibrium (LD) between SNPs, haplotype frequencies, estimations can be done using the Expectation-Maximization (EM) algorithm implemented in the computer program ARLEQUIN v. 2.0 ((Excoffier and Slatkin, Mol. Biol. Evol. 1995, 12 (5):921-927), and downloadable from http://lgb.unige.ch/arlequin/), an exploratory population genetics software environment.

[0194]Pair-wise measure of linkage disequilibrium (|D'|) can be calculated for all combinations of frequencies as described by R. C. Lewontin, Genetics 120, 849-52 (1988). A |D'| value of 1 indicates complete linkage disequilibrium between two markers.

[0195]Examples of useful statistical methods and techniques include Analysis of Variance (ANOVA), Fischer's test for pair-wise comparison and Wilcox's test, generally carried out using programs such as SPSS (Chicago, Ill.), STATVIEW and SAS (both available from SAS, Cary, N.C.).

EXAMPLE 1

[0196]Identifying and Isolating APOAV

[0197]Orthologous mouse genomic DNA was isolated from a pooled BAC library using the polymerase chain reaction (PCR) with mouse primers: apoAI-F1-5'-GAGGATGTGGAGCTCTACCGC-3' (SEQ ID NO:8) and apoAI-R1-5'-CTGTGTGCGCAGAGAGTCTACG-3') (SEQ ID NO:9) (RPCI-23, BACPAC Resources, Children's Hospital Oakland Research Institute; (See K. Osoegawa, et al., Genome Res 10, 116-28 (2000)). Positive clone RPCI-23-175F2 was identified, randomly sheared, sub-cloned and sequenced to approximately six-fold coverage according to methods described by I. Dubchak, et al., Genome Res 10, 1304-6 (2000) and G. G. Loots, et al., Science 288, 136-40 (2000). The sequence was deposited in GenBank (GenBank accession number AF401201). Human and mouse sequence comparisons were performed as previously described. Protein analyses were performed using the web-based Predict-Protein package, COILS (A. Lupas, et al., Science 252, 1162-4 (1991).), and SignalP (H. Nielsen, et al., Protein Eng 10, 1-6 (1997)).

[0198]The VISTA (www-gsd.lbl.gov/vista) graphical plot in FIG. 1B displays the level of homology between human and the orthologous mouse sequence spanning the apoAI/CIII/AIV cluster. Human sequence is represented on the x-axis and the percent similarity with the mouse sequence is plotted on the y-axis (ranging from 50-100% identity). Once the mouse sequence had been generated and the comparison obtained, a relatively high level of homology was observed in the region of the present APOAV, as can be seen from the plot.

[0199]To identify expression patterns of APOAV, mice were sacrificed and tissues harvested for either total RNA isolation using the RNA easy-midi protocol (Qiagen, Valencia, Calif.) or for polyA mRNA isolation using the FastTrack 2.0 system (Invitrogen, Carlsbad, Calif.). Approximately 10 μg of total RNA or 2 μg of polyA MRNA were separated in 1.0% agarose by gel electrophoresis and the RNA was transferred to a charged nylon membrane (Ambion, Austin, Tex.). The RNA blots were hybridized with [α-³²P]dCTP random-primed mouse apoA5 and human APOAV probes in ULTRAhyb buffer (Ambion, Austin, Tex.). Probe templates were generated by PCR amplification of liver cDNA using degenerate primers degApoAV-F2-5'-GCGCGTGGTGGGRGAAGACA-3' (SEQ ID NO:22) and degApoAV-R2-TCGCGCAGCTGGTCCAGGTT-3' (SEQ ID NO:23). Filters were washed in 2×saline sodium citrate at room temperature for 20 minutes and in 0.1×SSC at 42° C. for 20 minutes, followed by autoradiography visualization.

[0200]The results of the RNA blots are described herein. (A) A mouse apoA5 cDNA probe was hybridized to a multi-tissue RNA blot from wild-type mice. Each lane contained one of eight mouse tissues (Clontech, Palo Alto, Calif.), respectively: 1, heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; and 8, testis. The probes hybridized only to two transcripts approximately 1.3 and 1.9 kb in size in liver tissue (lane 5). (B) A human APOA5 cDNA probe was hybridized to a RNA blot containing eight human tissues (Clontech, Palo Alto, Calif.), respectively: 1, heart; 2, brain; 3, placenta; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; and 8, pancreas. The probes hybridized only to two transcripts approximately 1.3 and 1.9 kb in size in liver tissue (lane 5). (C) A human-specific APOA5 cDNA probe was hybridized to total RNA blots from human apoA5 transgenic mice and controls. Lane assignments are as follow: 1,5 transgenic liver; 2,6 transgenic intestine; 3,7 wild-type liver; 4,8 wild-type intestine. The probes hybridized only to two transcripts approximately 1.3 and 1.9 kb in size in transgenic liver tissue (lanes 1 and 5). (E) Northern blot analysis of various genotype mice using mouse APOAS probe following the apoA5 targeting event. Each lane contains liver mRNA from a wild-type (lane 1), heterozygous (lane 2) and homozygous knockout mouse (lane 3). To confirm similar amounts of RNA were loaded per lane, duplicate gels were examined by ethidium bromide staining. There was a large amount of transcript around 1.9-2 kb in lane 1 and a smaller band in lane 2 of same weight, while lane 3 showed no transcript.

EXAMPLE 2

[0201]Transgenic Non-Human Animals to Assess the Function of APOAV and apoAV

[0202]Restriction enzyme predictions for human genomic sequence (Genbank Accession Number AC007707) indicated that the entire human APOAV gene, but not neighboring genes, was contained within a 26 kbp XhoI DNA fragment (corresponding to approximately 1-27 kbp in FIG. 1B). BAC DNA corresponding to the clone sequenced from this region was prepared by standard alkaline lysis with a chromatography column (Qiagen, Valencia, Calif.), digested with the restriction enzyme XhoI and separated in 1% agarose by pulse-field gel electrophoresis. The 26 kbp XhoI DNA fragment containing human APOAV was purified using QIAEX II gel purification (Qiagen, Valencia, Calif.), adjusted to a final concentration of ˜1 ng/ml and micro-injected into fertilized FVB inbred mouse eggs using standard procedures. See K. A. Frazer, G. Narla, J. L. Zhang, E. M. Rubin, Nat Genet 9, 424-31 (1995).

[0203]Two founder transgenic mice were identified as determined by PCR amplification using primers hAPOA5-intrn-F1-5'-CCCGCTGCAGTCCCCAGAAT-3' (SEQ ID NO:10) and hAPOA5-intrn-R1-5'-CAGGGTCGAGGGCTCTTGTCCT-3' (SEQ ID NO:11). Each founder line was expanded by breeding to isogenic FVB strain mice (The Jackson Laboratory, Bar Harbor, Minn.).

[0204]The targeting construct to delete mouse apoA5 was built using PCR products amplified from BAC-RPCI-23-175F2 DNA (BACPAC Resources, Children's Hospital Oakland Research Institute). The first homology arm was PCR-amplified using primers containing introduced 5' restriction sites for XbaI and EcoRI, respectively: mAV-XbaI-F1-5'-TGACTCTAGATACCCTTGGTCCCATGTTCCAGAT-3' (SEQ ID NO:12) and mAV-EcoRI-R1-5'-CATTGAATTCGACAAGAGAAAGACGGGGCTCAAG-3' (SEQ ID NO:13). The resulting 4.2 kbp PCR product was cloned into pXL-Topo (Invitrogen, Carlsbad, Calif.), DNA prepared by standard alkaline lysis (Qiagen, Valencia, Calif.) and digested with EcoRI according to the manufacturer's recommendations (New England Biolabs, Beverly, Mass.). A 4.2 kbp EcoRI fragment was gel-purified and cloned into the EcoRI site of the pPN2T vector to yield pPN2T-Arm1 (C. Paszty, et al., Nat Genet 11, 33-39 (1995)). Clones were PCR screened for inserts using the above described primers and positive clones were sequenced for proper orientation.

[0205]The second homology arm was PCR-amplified using primers mAV-NotI-F4-5'-TATGACTGCGGCCGCCACCAATCCCACATCTAAGCATCT-3' (SEQ ID NO:14), containing an introduced 5' NotI restriction site, and mAV-XhoI-R3-5'-GCTCGGTTCTGGGCACAGAGA-3' (SEQ ID NO:15). The resulting 5.3 kbp PCR product containing an endogenous internal XhoI restriction site was digested with NotI and XhoI to yield a 5.1 kbp fragment which was directionally cloned into the XhoI and NotI sites of the pPN2T-Arm1 vector to yield final vector pPN2T-apoAV-KO. 129/SvJ ES cells (Incyte Genomics, Palo Alto, Calif.) were electroporated with 20 μg of the NotI linearized targeting construct and subsequently selected in 200 μg/ml G418 and 0.5 μg/ml FIAU for 8 days. Individual clones were isolated, expanded and screened by Southern blot analysis.

[0206]The external 3' probe was amplified by PCR using primers mApoAV-3' probe-F2-5'-CTTGAGGATGGGCATCAGCTGTAT-3' (SEQ ID NO:16) and mApoAV-3'probe-R2-5'-GCTCACTAACAGCGCTCTTGCCT-3' (SEQ ID NO:17). Targeted clones were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 females (The Jackson Laboratory, Bar Harbor, Minn.). Agouti offspring were tested for germline transmission of the targeted allele by PCR using primers specific to the neomycin gene (NeoF1-5'-CTTTTTGTCAAGACCGACCTG-3' (SEQ ID NO:37) and NeoR1-5'-AATATCACGGGTAGCCAACGC-3' (SEQ ID NO:38)) and heterozygous animals were intercrossed to obtain homozygous deletion animals for the mouse apoAS locus. Offspring were genotyped with PCR primers designed to the neomycin gene and with primers contained within the apoA5 deleted interval (mApoA5-F2-5'-ACAGTTGGAGCAAAGGCGTGAT-3' (SEQ ID NO:18) and mApoA5-R2-5'-CTTGCTCGAAGCTGCCTTTCAG-3' (SEQ ID NO: 19)). Properly targeted embryonic stems cells were identified using an external 3' probe which detects a 17 kb EcoRI fragment wild-type allele and a 10 kb EcoRI fragment upon targeting.

EXAMPLE 3

[0207]Plasma Triglyceride and Cholesterol Levels for APOAV Transgenic and Knockout Mice

[0208]Referring now to FIG. 3, results from the present human APOAV transgenic mice and the apoAV knockout mice are shown. Plasma triglyceride and cholesterol levels for apoAV transgenic and knockout mice on standard chow diet are illustrated. (A) Human APOAV transgenic mice compared to isogenic FVB strain control littermates (n=48 for transgenics; n=44 for controls; student t-test *p<0.0001 for transgenic versus control) have a ˜70% decrease in triglyceride levels. (B) Mice lacking APOAV compared to mixed 129Sv/C57B16 strain controls littermates (n=13 for wild-type, +/+; n=22 for heterozygotes, +/-; n=10 for homozygous knockouts, -/-; student t-test **p<0.001 for wild-type versus knockout) have a 400% increase in triglyceride levels. Error bars correspond to the standard deviation for both graphs.

[0209]The transgenic mice had approximately three-fold lower levels of plasma triglyceride when compared with control littermates ((0.32±0.11 (S.D.) mg/ml versus 0.90±0.29; T-test p<0.0001). Similar data were obtained from a second independent founder line (data not shown).

[0210]Mice lacking apoA5 were compared to mixed 129Sv/C57B16 strain control littermates (n=13 for wild-type, +/+; n=22 for heterozygotes, +/-; n=10 for homozygous knockouts, -/-; student t-test **p<0.001 for wild-type versus knockout) (FIG. 3B). Despite the lack of apoA5 transcript, mice homozygous for the deletion were born at the expected Mendeliai rate and appeared normal. In contrast to the decreased triglyceride levels noted in APOAV transgenics, apoA5 knockout mice had approximately four-fold higher levels of plasma triglyceride when compared with wild-type littermates (1.53±0.77 (S.D.) mg/ml versus 0.37±0.12; T-test p<0.001) (FIG. 3B). Error bars correspond to the standard deviation for both graphs.

[0211]Characterization of lipoprotein particles by fast protein liquid chromotography revealed that levels of very low density lipoprotein (VLDL) particles were increased in the homozygous knockout mice and decreased in the transgenic mice compared with controls. VLDL levels in a heterozygous knockout mouse were intermediate between the homozygous knockout and control mouse. The peak VLDL elution volumes were similar in all animals, indicating comparable VLDL particle size, and levels of other lipoproteins were not significantly altered.

EXAMPLE 4

[0212]Genotyping Human Individuals

[0213]Blood samples were collected after a 5-hour fast by retro-orbital bleeding using heparinized micro-hematocrit tubes. Total cholesterol and triglyceride concentrations were measured using enzymatic methods on a Gilford System 3500 analyzer (Gilford Instruments, Oberlin, Ohio).

[0214]For the entire genomic sequence of APOAV, overlapping sequence-tagged sites (STSs) of 400-498 bp in size were designed and tested using PCR-amplification on human genomic DNA as previously described in E. M. Beasley, R. M. Myers, D. R. Cox, L. C. Lazzeroni, PCR Applications (Academic Press, San Diego, Calif., 1999). Only primer pairs that resulted in a single PCR product of expected size were used for subsequent amplifications. For SNP discovery, STSs were PCR-amplified from eight samples of the Polymorphism Discovery Resource panel (PDR08, Coriell Cell Repository, Camden, N.J.), and products were purified through Millipore plates according to the manufacturer's recommendations (Millipore, Bedford, Mass.). Subsequent sequencing reactions with purified PCR products were performed using Big Dye Terminator chemistry and forward or reverse primers in separate sequencing reactions (Applied Biosystems, Foster City, Calif.).

[0215]Reactions were analyzed using a 3700 Sequence Analyzer (Applied Biosystems, Foster City, Calif.). Sequence traces were automatically analyzed using PhredPhrap and Polyphred (D. A. Nickerson, V. O. Tobe, S. L. Taylor, Nucleic Acids Res 25, 2745-51 (1997); B. Ewing, P. Green, Genome Res 8, 186-94 (1998)). For SNPs identified through this analysis, PCR INVADER assays (Third Wave Technologies, Madison, Wis.) were designed and tested on 90 samples from the Polymorphism Discovery Resource panel (PDR90) (C. A. Mein, et al., Genome Res 10, 330-43 (2000)). Successful assays were subsequently used to analyze samples from our study. Genotypes were assigned automatically by cluster analysis. Differences among genotypes were analyzed by one way ANOVA using STATVIEW 4.1 software (Abacus Concepts, Inc., Berkeley, Calif.).

[0216]To genotype the C/T SNP3 polymorphisms upstream of APOAV (discussed in Example 5), oligonucleotides AV6-F-5'-GATTGATTCAAGATGCATTTAGGAC-3' (SEQ ID NO:20) and AV6-R-5'-CCCCAGGAACTGGAGCGAAATT (SEQ ID NO:21) were used to amplify a 187 bp fragment from genomic DNA. The penultimate base in AV6-R was changed to T to create a MseI site (TTAA) in the common allele. The PCR reactions were performed in 20 μl volumes containing 50 mmol/l KCl, 10 mmol/l Tris (pH 8.3), 1.5 mmol/l MgCl₂, 0.2 mmol/l of each dNTP, 1 U of Taq, DNA polymerase and 200 pmol/l of each primer. DNA was amplified using the following conditions: initial denaturation of 96° C. for 2 min, followed by 32 cycles of 94° C. for 15 sec, 55° C. for 30 sec and 72° C. for 30 sec, and a final step at 72° C. for 3 min. 20 μls of PCR product were digested with 10 U of MseI (New England Biolab) at 37° C. for 3 h. The PCR products were size-fractionated on 3% agarose gels, stained with ethidium bromide and visualized on a UV transilluminator.

EXAMPLE 5

[0217]Human APOAV Polymorphisms and Lipid Association Studies

[0218]Referring now to FIG. 5, plasma lipid concentrations for a given genotype for four neighboring SNPs (SNPs1-4) are shown in Table 5A. For that study, 501 individuals were genotyped and the number of successfully scored individuals is approximately 430. The number of individuals of each SNP genotype is shown in row "n". In the row labelled "Genotype," 1,1=homozygous for the major allele; 1,2=heterozygous for the major and minor alleles. All individuals homozygous for the minor alleles of individual APOAV SNPs1-3 were removed from the analysis (n=2) to prevent their over-representation. All sites were found to be in Hardy-Weinberg equilibrium (data not shown). The minor allele frequency for each SNP (SNPs1-4) was 9.1, 8.4, 9.2 and 36.3%, respectively. Not shown is the lack of association between each of the four SNPs and IDL-, LDL-, HDL-mass, APOAI, and APOB levels (p>0.05, data not shown) FIG. 5B shows Pair-wise measure of linkage disequilibrium (|D'|) was calculated for all combinations of SNPs 1-4. A|D'| value of 1 indicates complete linkage disequilibrium between two markers. FIG. 5C shows a summary of SNP3 genotyping data from an independent set of individuals stratified based on triglyceride levels. P values were determined by Chi-square analysis. BMI=body mass index, TG=plasma triglyceride level (mg/dl±SEM).

[0219]Plasma lipid concentrations for a given genotype for four neighboring SNPs (SNPs1-4) are shown in FIG. 5A for triglycerides, VLDL, LDL and HDL. 501 unrelated normolipidemic Caucasian individuals who had been phenotyped for numerous lipid parameters before and after consumption of high- and low-fat diets were used in this study. Subjects were a combined subset of 501 healthy, nonsmoking Caucasian individuals aged >20 years (429 men, 72 women) who had participated in previous dietary intervention protocols (R. M. Krauss, D. M. Dreon, Am J Clin Nutr 62, 478S-487S (1995); D. M. Dreonet et al., Arterioscler Thromb Vasc Biol 17, 707-14 (1997)). All subjects had been free of chronic disease during the previous five years and were not taking medication likely to interfere with lipid metabolism. In addition, they were required to have plasma total cholesterol concentrations <6.74 mmol/L (260 mg/dL), triacylglycerol <5.65 mmol/L (500 mg/dL), resting blood pressure <160/105 mm Hg, and body weight <130% of ideal. Each participant signed a consent form approved by the Committee for the Protection of Human Subjects at EO Lawrence Berkeley National Laboratory, University of California, Berkeley, and participated in a medical interview. Fasting blood samples were obtained on their usual diets, and after 4-6 weeks of consuming diets containing high fat (35-46% energy) and low fat (20-24% energy). Plasma lipid and lipoprotein measurements were performed as previously described (R. M. Krauss, D. M. Dreon, Am J Clin Nutr 62, 478S-487S (1995); D. M. Dreonet al., Arterioscler Thromb Vasc Biol 17, 707-14 (1997)). In addition, on the high and low fat diets, total mass was measured by analytic ultra-centrifugation.

[0220]Significant associations were found between both plasma triglyceride levels and VLDL mass and the three neighboring SNPs (SNPs1-3) within APOAV but not with the distant upstream SNP4 (FIGS. 1A, 4A). Specifically, the minor allele of each of these SNPs (SNPs 1-3) was associated with higher triglyceride levels independent of diet. Independent analysis of each of these SNPs (SNP1-3) revealed plasma triglyceride levels were 20-30% higher in individuals having one minor allele compared to individuals homozygous for the major allele. Analysis of SNP allele frequencies in more than 1,000 chromosomes revealed that the three neighboring SNPs (SNPs1-3) in APOAV were in significant linkage disequilibrium that does not extend to SNP4 (located ˜11 kb upstream of APOAV). This finding supports the existence of a common haplotype in the APOAV region influencing plasma triglyceride levels (FIG. 4B). Furthermore, preliminary studies in this population found no significant association of triglyceride levels with a Sst1 polymorphism in APOC3 (located ˜40 kbp upstream of APOAV) which has been previously associated with severe hyper-triglyceridemia (See M. R. Hayden, et al., Am J Hum Genet 40, 421-30 (1987), M. Dammerman, et al., Proc Natl Acad Sci USA 90, 4562-6 (1993)). All individuals homozygous for the minor alleles of individual APOAV SNPs1-3 were removed from the analysis (n=2) to prevent their over-representation. All sites were found to be in Hardy-Weinberg equilibrium (data not shown). The minor allele frequency for each SNP (SNPs1-4) was 9.1, 8.4, 9.2 and 36.3%, respectively. No association between each of the four SNPs and IDL-, LDL-, HDL-mass, ApoAI, and ApoB levels (p>0.05) was observed.

[0221]Pair-wise measure of linkage disequilibrium (|D'|) was calculated for all combinations of APOAV SNPs as previously described by R. C. Lewontin, Genetics 120, 849-52 (1988). A |D'| value of 1 indicates complete linkage disequilibrium between two markers.

[0222]A summary of SNP3 genotyping data from an independent set of individuals stratified based on triglyceride levels. P values were determined by Chi-square analysis. BMI=body mass index, TG=plasma triglyceride level (mg/dl±SEM).

[0223]In a second human association study with SNP3 in an independently ascertained cohort using a different experimental design (FIG. 5C). SNP3 was chosen for genotyping in this study based on its strong association in our first study and its apparent complete linkage disequilibrium with the other two associated SNPs (SNPs 1-2). In the second study, we examined the allele frequencies for SNP3 in an unrelated group of Caucasians stratified according to plasma triglyceride levels. The two groups represented 115 individuals with triglyceride levels in the top tenth-percentile and 183 individuals from the bottom tenth-percentile. A significant over-representation of the heterozygous genotype (SNP3, APOA5*2) was found in individuals with high- compared to low-plasma triglyceride levels (18.3% versus 8.7%, respectively), thereby validating the effect in a second cohort. When the cohort was stratified based on gender, an even more pronounced over-representation of the heterozygous genotype was found in males with high- compared to low-plasma triglyceride levels (29.4% versus 5.2%, respectively).

[0224]Individuals that carry either of two independent SNPs described above have ˜30% higher triglyceride levels. Population-wide this effect is large. 25% of Caucasians, 36% of African-Americans, and 51% of Hispanics carry at least one copy of these two alleles associated with elevated triglycerides.

EXAMPLE 6

[0225]APOAV Haplotypes: Linkage Disequilibrium and Association Studies

[0226]The present Example describes methods for establishing genetic profiles of individuals carrying various alleles of the present APOAV gene. These methods rely on Linkage Analysis studies and result in the identification of haplotyes including the SNP's described in connection with FIG. 5. The haplotypes are illustrated in FIG. 4B and are APOA5*1, APOA5*2 and APOA5*3.

[0227]The present study protocols were approved by the appropriate institutional review boards. Fasting blood samples were obtained from i) 116 hyperlipidemic patients including 34 with Type III hyperlipidaemia, 10 with familial combined hyperlipidemia, 24 with LDL cholesterol levels exceeding the 90^th percentile, and 48 patients with plasma triglyceride levels exceeding 500 mg/dl; ii) 82 Caucasian men and 50 Caucasian women who were homozygous for the common allele of SNP3 (-1131T) and who had plasma triglyceride concentrations above the 90^th percentile for age and sex, and an equal number who were homozygous for the common allele of SNP3 (-1131T) and had plasma triglyceride concentrations below the 10^th percentile for age and sex; and iii) 2660 residents of Dallas County selected at random from census tracts who participated in the Dallas Heart Disease Prevention Project (DHDPP), a population-based study of atherosclerotic heart disease.

[0228]DNA samples were also obtained from healthy, nonsmoking, Caucasian men (n=354) and women (n=65) who had participated in previous dietary intervention protocols and had plasma cholesterol levels below 260 mg/dl and plasma triglyceride levels below 500 mg/dl.

[0229]DNA sequencing: The exons and flanking intron sequences of the APOAV gene were screened for sequence polymorphisms by DNA sequencing. DNA fragments of ˜400 basepairs spanning each exon were PCR amplified and sequenced using Big Dye Terminator Cycle Sequencing reagents on an ABI3100 automated sequencer.

[0230]SNP genotyping: The SNP5 (S19W) and V153M polymorphisms were assayed using PCR-RFLP and PCR INVADER assays (Third Wave Technologies, Madison, Wis.) as described previously. All PCR primers and probes used in biplex INVADER assays for this study are listed in Table 4. To assay the SNP5 polymorphism, oppositely-oriented oligonucleotides (AV1-F 5' TGCTCACCTGGGCTCTGGCTCTTC (SEQ ID NO:24) and AV1-R 5' CCAGAAGCCTTTCCGTGCCTGGGCGGC (SEQ ID NO:25)) were designed with a single nucleotide mismatch such that the C to G substitution that changes codon 19 from serine to tryptophan creates an Eag I site. PCR was performed in 20 μl volumes containing 50 mM KCl, 10 mM Tris (pH 8.3), 1.5 mM MgCl₂, 0.2 mM of each dNTP, 1 U of Taq DNA polymerase and 200 pM of each primer. Reactions were performed in a PTC-200 Thermal cycler (MJ Research, South San Francisco, Calif.) using an initial denaturation step of 96° C. for 2 min, followed with 30 cycles of 94° C. for 15 sec, 70° C. for 20 sec and 72° C. for 30 sec. The PCR products were digested for 3 hr at 37° C. with 7 U of Eag I (New England Biolabs, Beverly, Mass.) in buffer provided by the manufacturer and analysed by electrophoresis in 3% agarose gels. For the V153M polymorphism, genomic DNA was amplified using the oligonucleotides AV150-R 5' TGGTGCACCACGAGGCTCTGCAGCAGTCCC (SEQ ID NO:26) and AV150-F 5' AGGTGGCCCTGCGAGTGCAGGAGCTGC (SEQ ID NO:27) as described above, except that the annealing temperature was 67° C. PCR products were digested with Nla III and assayed by electrophoresis in 3% polyacrylamide gels.

[0231]The SNP3 polymorphism was analyzed by mass spectrometry using the MASSARRAY system (Sequenom Corporation, San Diego, Calif.) (Buetow et al. 2001, Proc. Natl. Acad. Sci. U.S.A 98 (2):581-584). The oligonucleotides used in biplex INVADER genotyping assays (Sequenom Corporation, San Diego, Calif.) are shown in Table 4 below and are SEQ ID NOS: 28-36. The polymorphisms SNP5, SNP6, and V153M (location shown in FIG. 4A) are available in dbSNP under accession numbers ss4383597, ss4383596, and ss4383598, respectively and in GenBank under rs3135506 (SNP5), rs651821 (SNP6), and rs3135507 (V153M).

TABLE-US-00004 TABLE 4 SNP Sequence SEQ ID NO: 28 SNP 6 Probe 1 ATG ACG TGG CAG ACG TAA TGG CAA GCA TGG C SEQ ID NO: 29 Probe 2 CGC GCC GAG GAT AAT GGC AAG CAT GGC SEQ ID NO: 30 Invader GCC TCC CTC CAC CTG TCT TCT CAG AGC AGT SEQ ID NO: 31 SNP5 Probe 1 ATG ACG TGG CAG ACG AAA ACG CTG TGG AGA G SEQ ID NO: 32 Probe 2 CGC GCC GAG GCA AAA CGC TGT GGA GAG SEQ ID NO: 33 Invader GCC TTT CCG TGC CTG GGT GGC CT SEQ ID NO: 34 V153M Probe 1 ATG ACG TGG CAG ACG TGG TGG GGG AAG AC SEQ ID NO: 35 Probe 2 CGC GCC GAG GAT GGT GGG GGA AGA C SEQ ID NO: 36 Invader AGG AGC TGC AGG AGC AGT TGC GCT

[0232]Statistical Analysis: Statistical analyses were carried out using the SAS computer program (Cary, N.C.). Plasma triglyceride concentrations were compared among different genotype groups using Wilcoxon's test. Allele frequencies were compared using Fisher's exact test. To determine pairwise linkage disequilibrium (LD) between SNPs, haplotype frequencies were estimated for 353 unrelated individuals using the Expectation-Maximization (EM) algorithm implemented in the computer program ARLEQUIN v. 2.0 (Excoffier and Slatkin, Mol. Biol Evol. 1995, 12 (5):921-927). The resulting frequencies were used to calculate the pairwise LD parameter D' as discussed by Lewontin (Genetics 1988, 120 (3):849-852).

[0233]DNA sequencing: Screening of the coding regions and intron-exon boundaries of APOAV in 116 hyperlipidemic individuals revealed 10 new DNA sequence variations (FIG. 1A). An A to G substitution 3 nucleotides upstream of the initiation codon (SNP6) was found to be in strong linkage disequilibrium with three previously described polymorphisms (SNP1, SNP2, SNP3, FIG. 1A) that define the APOA5*2 haplotype which is associated with increased plasma triglyceride concentrations (Pennacchio et al., 2001 Science 294 (5540):169-173). The A to G substitution results in a conservative change in the predicted Kozak consensus sequence (SNP6) (Kozak 1991, J Cell Biol 115 (4):887-903; Kozak, Cell 1986, 44 (2):283-292). Two common nonsynonymous substitutions were also identified: A C→G substitution (SNP5) changed codon 19 from serine to tryptophan in 23 individuals, and a G→A substitution (c.457G>A) changed codon 153 from valine to methionine in 14 individuals. A third nonsynonymous substitution (c.944C>T) that changed codon 315 from alanine to valine was identified in two hyperlipidemic individuals. This conservative substitution did not co-segregate with hyperlipidemia in the family members of one of these individuals (data not shown) and was not found in 108 normolipidemic individuals, therefore no further studies of this polymorphism were undertaken. The other six polymorphisms, including three silent substitutions (c.132C>A, c.695C>G, c.738C>T), and three polymorphisms each found only in single individuals (IVS2+55G>C, and c. 1132C>T and c.1156 G>A in the 3' UTR) were not evaluated further.

[0234]Allele frequency and Linkage Disequilibrium: Five polymorphisms were found to define three common haplotypes (denoted APOA5*1, APOA5*2, and APOA5*3) in 419 unrelated Caucasian individuals (FIG. 4B). These three haplotypes represented 82%, 8%, and 8% of the APOAV chromosomes examined, and thus comprise more than 98% of APOAV haplotypes in this population. APOA5*2 is distinguished from the common haplotype (APOA5*1) by four nucleotide substitutions (-1131T>C, c.-3A>G, IVS3+476G>T, and c.1259T>C) and shown to be associated with increased plasma triglyceride levels and named SNP3, SNP6, SNP5, SNP2 and SNP1 respectively. APOA5*3 is distinguished from the common haplotype by the substitution of G for C at nucleotide c.56 (codon 19 in the amino acid sequence). To determine the relative frequencies of the APOA5*2 haplotype in African-Americans and Hispanics, the -1131T>C SNP5 polymorphism was assayed in 1031 randomly selected individuals, including 545 African-Americans, 152 Hispanics, and 334 Caucasians. The allele frequency was significantly higher in African-Americans (0.12) and Hispanics (0.12), than in Caucasians (0.06, P<0.001). The frequency of the W19 allele (which defines haplotype APOA5*3) was similar in African-Americans (0.07) and Caucasians (0.06), but was substantially higher in Hispanics (0.15, P<0.001 compared to African-Americans).

[0235]Using this mathematical calculation specifically for SNP6 (APOA5*2) where the minor allele frequency is 6% in Caucasians, we find the distribution is 88% homozygous major, 11.6% heterozygous, 0.4% homozygous minor. Similarly, for SNP5(APOA5*3) the minor allele frequency is 6% for Caucasians, thus the distribution is 88% homozygous major, 11.6% heterozygous, 0.4% homozygous minor. Therefore, because SNP5 and SNP6 are independent of each other, 23.2% of the population is heterozygous (because 11.6%+11.6%=23.2%) and an additional 0.8% are homozygous for the minor allele. Thus, a large number (24%) of individuals in the general Caucasian population have elevated triglyceride levels solely due to the effect of APOAV polymorphisms.

[0236]In addition to APOAV's strong association with triglyceride levels in Caucasians, a strong effect is also seen African-Americans and Hispanics where the minor allele frequencies are higher. Thus, a larger percent of African-Americans and Hispanics display increased triglycerides due to the genetic effect of APOAV. Specifically, APOA5*2 and/or APOA5*3 are present in 36% of African Americans and 51% of Hispanics and results in an ˜25% increase in triglycerides compared to APOA5*1 homozygotes.

[0237]For SNP6 (APOA5*2) in Hispanics and African-Americans, the minor allele frequency is 12% thus the distribution is 77.4% homozygous major, 21.1% heterozygous, 1.4% homozygous minor. For SNP5 (APOA5*3) in Hispanics, minor allele frequency is 15%. Thus the distribution is 72% homozygous major, 25.5% heterozygous, 2.3% homozygous minor. For African-Americans, SNP5 (APOA5*3) minor allele frequency is 7% thus the distribution is 86.7% homozygous major, 13.0% heterozygous, 0.5% homozygous minor. Thus, for Hispanics 23.5% of individuals carry APOA5*2 and 27.8% carry APOA5*3 for a total of 51.3% of Hispanics carry minor versions of APOAV associated with increased triglycerides. Using a similar logic, 36% of African-Americans carry minor versions of APOAV associated with increased triglycerides.

[0238]Abnormalities in APOAV may be solely responsible for human genetic forms of cardiovascular disease (similar to APOE or APOAI) in certain families and individuals. By screening this gene in families with individuals segregating cardiovascular or other types of disease, causative mutations may be found. This would have important diagnostic implications as well as provide therapeutic entry points. Furthermore, the data indicate that alleles in this gene are associated with increased plasma triglyceride levels thereby likely predisposing large numbers of individuals to increased susceptibility to coronary artery disease. A second implication of our findings is that this gene has sequence variations or single nucleotide polymorphisms that correlate to increased susceptibility to cardiovascular disease. The minor alleles of the polymorphisms disclosed herein associated with triglycerides occur in approximately 25% of the Caucasian population, 36% of African-Americans and 51% of Hispanics, thus representing a significant cross-section of the population. There is an approximate 25% chance that a Caucasian person is heterozygous for one or both of the two rare haplotypes and individuals having this rare allele have 20-30% higher triglyceride levels. Furthermore, the present studies suggest that the rare allele at the SNP5 locus (or any polymorphism in linkage disequilibrium with it) may have a major impact on plasma triglyceride levels in those persons predisposed to hypertriglyceridemia. Therefore, finding and exploring the significance of DNA sequence polymorphisms in APOAV and its subsequent effect on plasma triglyceride levels in humans is another important diagnostic implication of this embodiment.

[0239]Association Studies: To test for association between the two common, nonsynonymous polymorphisms identified in this study (SNP5 and V153M) and plasma triglyceride concentrations, the allele frequencies at these loci were compared in Caucasian men and women who had plasma triglyceride concentrations above the 90^th percentile or below the 10^th percentile for age and sex. To eliminate confounding by the APOA5*2 haplotype that was previously associated with high plasma triglyceride levels, individuals who carried this haplotype were excluded. In both sexes, the rare allele at codon 19 (W19) was significantly more common in individuals with plasma triglyceride levels above the 90^th percentile than in those with plasma triglyceride levels below the 10^th percentile (Table 5). Since individuals with the SNP3 allele were excluded, the association between the S19W (SNP5) polymorphism and plasma triglyceride concentrations is independent of the APOA5*2 haplotype that was previously shown to be associated with increased plasma triglyceride levels (Pennacchio et al. 2001).

[0240]The study showing the relationship between gender, genotype and triglyceride levels looked at men and women with high (>90^th percentile) and low (<10^th percentile) plasma triglyceride concentrations and is shown below.

TABLE-US-00005 TABLE 5 APOAV genotype S19/S19 S19/W19 W19/W19 P value Men TG < 10^th percentile 74 7 1 <0.005 (n = 82) (90.5) (8.5) (1) Men TG > 90^th percentile 63 19 0 (n = 82) (77) (23) (0) Women TG < 10^th percentile 50 0 0 <0.001 (n = 50) (100) (0) (0) Women TG > 90^th percentile 39 11 0 (n = 50) (78) (22) (0)

[0241]Values are numbers of individuals in each group. S19 is the common allele of SNP5. The percentage of individuals with the genotype is given in parentheses. All individuals were homozygous for the common alleles at SNPs 3, 6, 2 and 1, which means that all individuals with APOA5*2 haplotype were excluded. P values were calculated using Fisher's exact test.

[0242]To further assess this association, the SNP5 polymorphism was assayed in 419 healthy independently-ascertained Caucasians (354 men and 65 women). Baseline blood samples were obtained from these individuals on their self-selected diets, and additional samples were drawn following the consumption of a defined high-carbohydrate or high-fat diet. On all three diets, individuals who were heterozygous for the SNP5 (W19) allele and who lacked haplotype APOA5*2 had significantly higher plasma triglyceride concentrations than did individuals homozygous for the S19 (wild type) allele (Table 5).

[0243]The increase in mean plasma triglyceride levels associated with a single copy of the W19 allele was ˜36%, which is similar to the increase in triglyceride levels associated with APOA5*2 haplotype (˜32%) in these individuals. To determine if the W19 allele was associated with increased plasma triglyceride concentrations in other ethnic groups, the S19W polymorphism was assayed in a random sample of 1392 African-American, 420 Hispanic, and 848 Caucasians. In both sexes of all three ethnic groups, both the mean and the median plasma triglyceride concentrations were higher in W19 heterozygotes than in S19 homozygotes (Table 6 below). The difference was significant at the 0.05 confidence level for African-Americans and Caucasians in both sexes, but did not achieve the nominal significance threshold in Hispanics, presumably due to the smaller sample size in this group.

TABLE-US-00006 TABLE 6 Plasma triglyceride levels (mg/dl) S19/S19 S19/W19 W19/W19 P Value African- Mean ± S.D. 101 ± 169 131 ± 120 141 ± 50 0.0023 American Median ± I.Q. 80 ± 59 97 ± 85 192 Women range n (707) (108) (6) African- Mean ± S.D. 132 ± 152 176 ± 319 264, 84 0.024 American Median ± I.Q. 94 ± 84 111 ± 92 Men range n (494) (75) (2) Hispanic Mean ± S.D. 143 ± 95 174 ± 209 394 ± 534 0.057 Women Median ± I.Q. 119 ± 92 135 ± 99 214 range n (185) (57) (7) Hispanic Mean ± S.D. 173 ± 139 204 ± 182 206, 124 0.087 Men Median ± I.Q. 139 ± 108 157 ± 101 range n (119) (50) (2) Caucasian Mean ± S.D. 124 ± 96 147 ± 90 237, 125 0.012 Women Median ± I.Q. 100 ± 87 122 ± 110 range n (386) (54) (2) Caucasian Mean ± S.D. 161 ± 121 255 ± 225 0.0012 Men Median ± I.Q. 126 ± 116 183 ± 237 range n (362) (44) (0)

[0244]The nucleotide substitution (c. 457G>A, ) that changed codon 153 from valine to methionine was less common in men with high plasma triglyceride levels (3/82) than in men with low plasma triglyceride levels (7/82), but this difference was not statistically significant (P=0.12, Fisher's exact test). In 388 healthy Caucasian individuals, the mean plasma triglyceride level of 457G homozygotes was similar to that observed in 457GA heterozygotes (126.2±4.2 mg/dL (n=363) and 113.3±12.7 mg/dL (n=25), respectively, p=0.43).

EXAMPLE 7

[0245]Haplotype Linkage of APOAV Rare Alleles to Hyperlipidemia (CHL) and Familial Combined Hyperlipidemia (FCHL) Diseases

[0246]Familial combined hyperlipidemia (FCHL) is a common disorder of lipid metabolism affecting 1-2% of individuals in Western society. The term FCHL was coined by Goldstein et al (1973) to describe a pattern of lipid abnormalities in 47 Seattle pedigrees, ascertained through survivors of myocardial infarction who had raised blood cholesterol and triglyceride levels. Herein, APOAV allele SNP6 (c.56G) is shown to have an increased transmission in affected FCHL members from large pedigrees.

[0247]This example involves linkage and linkage disequilibrium (LD) tests on the APOA1/C3/A4/A5 genomic interval in a substantial cohort of white British families with FCHL. The results show that the transmission of FCHL in a subset of these families is linked to the transmission of two independent haplotypes in the APOA1/C3/A4/A5 genomic interval. The first haplotype contains the rare allele at the SNP5 locus and a second the rare allele at the APOC3^c.386C>G locus within the APOC3 (or "APOCIII") gene.

[0248]To establish the contribution of allelic variation at the APOA1/C3/A4/AS genomic interval to FCHL susceptibility, linkage and LD tests were performed on a cohort of white British families. For the linkage test, 86 extended families were genotyped with two markers: D11SAPOC3 which resides within the third intron of the APOC3 gene, and D11S1998, which is located approximately 1.7 Mbp downstream of the APOAV gene (FIG. 1). The families contained 177 and 270 affected relative pairs for the CHL phenotype and the triglyceride trait of FCHL, respectively. The D11SAPOC3 marker produced nominal evidence for linkage (NPL.sup.+ 1.72, P=0.042) of the chromosome 11q23 genomic region to the triglyceride trait of FCHL, and this was attributable to an excess of allele sharing in the affected pedigree members of a subset (i.e. 35) of the 86 families.

[0249]To substantiate evidence for linkage of the APOA1/C3/A4/A5 genomic interval to FCHL, we performed a PDT on 115 white British families using seven SNPs that span an interval of 108 Kbp, followed by a case-control study involving 181 white British probands and 268 pedigree founders. The "PDT," pedigree disequilibrium test, is described at "A Test for Linkage and Association in General Pedigrees: The Pedigree Disequilibrium Test" by Martin E R, Monks S A, Warren L L, and Kaplan N L. Am J Hum Genet 67:146-154, 2000. For a discussion of the SNP naming conventions used in this Example, see Antonarakis et al. "Recommendations for a nomenclature System for Human Gene Mutations," Human Mutation 11:1-3 (1998).

[0250]The SNPs are named using the annotation described previously, "IVS" means that the SNP is positioned in the intervening sequence, "c" means the SNP is positioned in the coding sequence, "-" indicates the location is upstream by a specified number of base pairs, and "+" indicates that the location is downstream by a specified number of base pairs.

[0251]The SNPs included two SNPs within the APOAV gene (SNP5 (APOA5^c.56C>G) and SNP6 (APOA5^c.-3A>G)), a noncoding SNP within the APOC3 gene (APOC3^c.386C>G), three SNPs upstream of the APOAV locus (SNP3 (APOA5^-1,131T>C), SNP4 (APOA5^-12,238T>C), and APOA1^-3031C>T) and one SNP (APOA5.sup.58,892C>T) downstream of APOAV.

[0252]The results of the PDT produced evidence for increased transmission of the rare alleles at the SNP5 and APOC3^c.386C>G loci to affected subjects. For example, the rare alleles at the SNP5 and APOC3^c.386C>G loci were respectively transmitted 1.95- and 1.45-fold more frequently to affected family members with the triglyceride trait of FCHL than an unaffected child individuals. The corresponding values for the CHL trait were 1.95 and 1.33, respectively. The rare alleles at the SNP6 and SNP3 loci were also transmitted 1.28 and 1.40 fold-more frequently to affected individuals with the CHL phenotypes of FCHL than to unaffected individuals (P=0.039 and 0.033).

[0253]The rare alleles at the SNP5, APOC3^c.386C>G, SNP3 and SNP6 loci were also present at increased frequencies in FCHL probands versus pedigree founders (i.e. "married ins"). For example, the rare allele at the SNP5 locus was present in 21% of the probands compared to 13% of the normolipidemic pedigree founders, whereas the rare allele at the APOC3^c.386c>G locus was present in 29% of the probands and 15% of the normolipidemic pedigree founders. Importantly, the results from this case-control study and the PDT complemented each other. For the example, the frequencies of the rare alleles at the SNP5, SNP6, SNP3 and APOC3^c.386C>G loci in FCHL probands and affected FCHL sibs were remarkably similar (i.e. 0.1200, 0.1144, 0.1111 and 0.1486, respectively versus 0.1114, 0.1156, 0.1296 and 0.1566). Likewise, the frequencies of the rare alleles at the SNP6 and APOC3^c.386C>G loci were similar in the pedigree founders and unaffected sibs (0.0694 and 0.1024, respectively versus 0.0511 and 0.1048) (Table 2). Thus, the case-control data support the evidence that the rare allele at SNP5 (APOA5^c.56G) and APOC3^c.386G alleles (or alleles in LD) are preferentially transmitted in FCHL.

[0254]Probands with the rare allele at SNP5 (APOA5^c.56G) had higher mean triglyceride levels than probands homozygous for the major allele at this locus, and this was particularly evident in those individuals that were homozygous for this rare allele (n=5). Thus, mean plasma triglyceride levels in probands with the APOA5^c.56G allele were on average 2.2 fold higher than in probands homozygous for the common SNP5 (APOA5^c.56C) allele, and ˜1.8 fold higher relative to the heterozygote probands. By contrast, the APOA5^c.56G allele had no major impact on triglyceride levels in heterozygote pedigree founders, and this was also the case when all individuals with the rare allele at the APOC3^c.386C>G locus were excluded from the analyses (data not shown). Only one pedigree founder was homozygous for the APOA5^c.56G allele, precluding an assessment of the impact of the homozygous state of this allele (or an allele in LD) on plasma triglyceride in the pedigree founders of white British families with FCHL.

[0255]The APOC3^c.386G allele (or an allele in LD) had a modest impact on triglyceride levels in probands and pedigree founders. On average pedigree founders with the APOC3^c.386G allele had plasma triglyceride levels that were 31% higher than pedigree founders without this allele (P=0.001), and this increased to a value of 38% (P=0.001) when we considered only those individuals with the common allele at the SNP5 locus (data not shown). Similar increases in plasma triglyceride levels were also observed in pedigree founders with the rare alleles at the SNP3 and SNP6 loci (data not shown). In a complementary analysis, increased frequencies of these rare alleles were observed in pedigree founders that had plasma cholesterol and triglyceride levels >75^th percentile age-sex-specific values relative to the rest. This trend was not observed for the rare allele at the APOA5^c.56C>G locus, indicating that this allele resides on a different APOA1/C3/A4/A5 haplotype than the rare alleles at the APOC3^c.386C>G and SNP3 and SNP6 loci.

[0256]To further test for preferential transmission of the APOA5^c.56G and APOC3^c.386G alleles in FCHL, a second study repeated the PDT in families with haplotype data for the APOA1/C3/A4/A5 genomic interval. The distorted transmission of the rare allele at the SNP5 locus in FCHL was restricted to the 35 families that produced evidence for linkage of the chromosome 11q23 locus to FCHL (P=0.0133), suggesting that a major component of this observed linkage may be explained by this allele, or a polymorphism in LD with it or a linked allele. By contrast, the rare alleles at the APOA5.sup.58892C>T, SNP6, SNP3 and APOC3^c.386C>G loci were only modestly over-transmitted in the 35 families that had contributed to the nominal evidence of linkage of FCHL to chromosome 11q23 (P=0.0423, 0.12, 0.19, 0.079, respectively), indicating supporting that these alleles, or alleles in LD with them, may have contributed at most very modestly effects to the observed linkage signal.

[0257]The results of the case-control study, included genotype data from 181 white probands and 268 pedigree founders, and essentially mirrored the results of the PDT. Thus, the frequencies of the rare alleles at the SNP5, APOC3^c.386C>G, SNP3 and SNP6 loci were increased in FCHL probands versus pedigree founders. For example, the rare allele at the SNP5 locus was present in 21% of the probands compared to 13% of the normolipidemic pedigree founders (P=0.01), whereas the rare allele at the APOC3^c.386C>G locus was present in 29% of the probands and 14.8% of the normolipidemic pedigree founders (P=0.01). The corresponding values for the rare alleles at the SNP3 and SNP6 loci were 20.5% and 6.4% (P=0.001), and 21.6% and 8.6% (P=0.001), respectively.

[0258]Probands with the rare allele at the SNP5 locus had higher mean triglyceride levels than probands homozygous for the major allele at this locus, and this was particularly evident in those individuals that were homozygous for this rare allele. Thus, mean plasma triglyceride levels in probands with the rare allele at the SNP5 locus were on average 2.2 fold higher than in probands homozygous for the common allele at this locus, and ˜1.8 fold higher than those found in the heterozygote probands. By contrast, the rare allele at the SNP5 locus had no major impact on triglyceride levels in heterozygote pedigree founders, and this was also the case when all individuals with the rare allele at the APOC3^c.386C>G locus were excluded from the analyses. Only one pedigree founder was found to be homozygous for the rare allele at the SNP5 locus, and this has precluded us from establishing the impact of the homozygous state of this allele (or polymorphisms in LD with it) on plasma triglyceride in the pedigree founders. Nonetheless, the inventors suggest that the rare allele at the SNP5 locus (or any polymorphism in LD with it) may have a major impact on plasma triglyceride levels in those persons predisposed to hypertriglyceridemia.

EXAMPLE 8

[0259]Modulating and Regulating APOAV Expression with Drugs

A. Human APOAV Gene Expression Induced by Fibrates Treatment

[0260]Fibrates are described at Miller D B, Spence J D. "Clinical pharmacokinetics of fibric acid derivatives (fibrates). Clin Pharmacokinet 1998; 34(2):155-62. To determine whether fibrates can modulate APOAV gene expression in humans, first, analyze APOAV mRNA levels in primary hepatocytes upon treatment with a fibrate such as fenofibric acid, the active form of fenofibrate, which is a prototype of PPARα ligands. Observe whether treatment with fenofibric acid at a concentration (100 μM) similar to that reached in plasma from patients treated with fenofibrate dramatically increases APOAV mRNA levels. These observations could demonstrate that fibrates induce the expression of the human APOAV, thus supporting the use of APOAV as a new target gene for fibrates. In general, a drug candidate may be tested in cells or animals and the effect of that drug on levels of APOAV mRNA and/or protein observed. Drug candidate which increase such levels have utility as agents which may lower cholesterol and triglyceride levels in appropriate subjects. As described in connection with Example 9, a drug candidate may also have insulin modulating properties which can be measured through the effect of a drug candidate on protein and/or mRNA levels of APOAV in a test animal or cell. Furthermore, which it is shown by the present work that increased levels of APOAV are associated with lowered levels of triglycerides and cholesterol, for individuals having deleterious alleles, lowering APOAV levels may be beneficial.

B. Fenofibrate Regulation of apoA5 Expression Via PPARα Activation

[0261]Next, one could examine whether PPARα is involved in the regulation of apoa5 by fibrates. Apoa5 mRNA levels could be strongly enhanced in the liver of wild-type mice after treatment with fenofibrate mixed in diet (0.2% w/w). These experiments would show that apoa5 expression is induced by fibrates in vivo in mouse liver and its regulation may largely depend on PPARα activation.

C. Gene Regulation of APOAV by Fibrates at the Transcriptional Level

[0262]To delineate the mechanism of regulation of APOAV gene expression by fibrates, functional analysis of the APOAV promoter is necessary. Host cells, such as HepG2 cells, can be transiently transfected with a Luciferase reporter vector driven by the human APOAV promoter and challenged with a PPARα activator. Transcriptional activity of the APOAV reporter construct can be observed for increase in levels after addition of the activator. Co-transfection with PPARα may also have the effect of strongly stimulating APOAV promoter activity. Such results would indicate that the gene regulation of APOAV by fibrates occurs at the transcriptional level.

D. APOAV Responsiveness to PPARα or PPARγ

[0263]Transcriptional activation of APOAV gene by PPARα would suggest the presence of a peroxisome proliferator-activated response elements (PPRE) in the APOAV promoter sequence. Comparative sequence analysis of the murine and human APOAV promoters can be performed to reveal the presence of any regions of cross-species conservation containing putative PPREs with a high degree of homology between the putative PPREs and the PPRE consensus defined for PPARs.

[0264]To assess whether the putative PPREs mediate any PPARα or PPARγ effects, one should perform transfection experiments using a promoter construct containing mutated versions of any PPREs found. If mutation of any PPREs found abolishes activation of the APOAV promoter by PPARα this would indicate that the human APOAV promoter contains PPREs that act to mediate PPAR action.

EXAMPLE 9

[0265]APOAV Expression Levels and Their Effect Upon Human Insulin Levels

[0266]Significant differences in plasma triglyceride concentrations in APOAV genetically engineered animals prompted study to determine if alterations in APOAV expression led to changes in plasma glucose or insulin levels as well. Significant differences were also found for plasma insulin but not glucose levels. APOAV transgenic mice were found to have ˜80% higher insulin levels than controls, compared to ˜220% lower insulin levels in apoA5 knockouts compared to controls. P-values were calculated based on student T-tests. Plasma glucose levels were also examined and no differences were found. Their levels were 173, 166, 132, and 130 mg/dL in ApoA5 transgenics, littermate controls, apoA5 knockouts, and littermate controls, respectively.

TABLE-US-00007 TABLE 7 Plasma Triglyceride and Insulin Concentrations in ApoA5 Transgenic, ApoA5 Knockout and Littermate Control Mice Insulin Triglycerides ng/ml (S.E.M.) p value (S.E.M.) p value Control (FVB) 152.9 (±6.3) 0.000015 0.9 (±0.07) 0.01 APOA5 90 (±7.6) 1.6 (±0.29) Transgenic (FVB) Control 150.3 (±26.1) 0.025 2 (±0.16) 0.0000006 (C57B16/129Sv) ApoAV Knockout 245.9 (±41.0) 0.9 (±0.06) (C57B16/129Sv)

[0267]The fact that both transgenic and knockout mice shown differences in insulin but not glucose levels indicate alterations in the function of insulin in these two models. For instance the finding of high triglycerides in APOAV transgenic (yet unchanged glucose levels) supports the hypothesis that these animals are insulin resistant.

[0268]These findings suggest that APOAV may plan an important role in metabolic syndrome, insulin resistance, obesity, and diabetes. In addition, they support that therapies directed at modulating APOAV levels or course of action may be useful for treating these common conditions in humans.

[0269]Thus there has been described in detail the making and use of the preferred embodiments of the present invention. In view of the present teachings, numerous alternatives and variations may be envisioned by one of ordinary skill in the field. Thus it is intended that the scope of protection for the present invention be limited only by the scope of the appended claims.

Sequence CWU 1

4811334DNAHomo sapiens 1gagcagagca gataatggca agcatggctg ccgtgctcac ctgggctctg gctcttcttt 60cagcgttttc ggccacccag gcacggaaag gcttctggga ctacttcagc cagaccagcg 120gggacaaagg cagggtggag cagatccatc agcagaagat ggctcgcgag cccgcgaccc 180tgaaagacag ccttgagcaa gacctcaaca atatgaacaa gttcctggaa aagctgaggc 240ctctgagtgg gagcgaggct cctcggctcc cacaggaccc ggtgggcatg cggcggcagc 300tgcaggagga gttggaggag gtgaaggctc gcctccagcc ctacatggca gaggcgcacg 360agctggtggg ctggaatttg gagggcttgc ggcagcaact gaagccctac acgatggatc 420tgatggagca ggtggccctg cgcgtgcagg agctgcagga gcagttgcgc gtggtggggg 480aagacaccaa ggcccagttg ctggggggcg tggacgaggc ttgggctttg ctgcagggac 540tgcagagccg cgtggtgcac cacaccggcc gcttcaaaga gctcttccac ccatacgccg 600agagcctggt gagcggcatc gggcgccacg tgcaggagct gcaccgcagt gtggctccgc 660acgcccccgc cagccccgcg cgcctcagtc gctgcgtgca ggtgctctcc cggaagctca 720cgctcaaggc caaggccctg cacgcacgca tccagcagaa cctggaccag ctgcgcgaag 780agctcagcag agcctttgca ggcactggga ctgaggaagg ggccggcccg gacccccaga 840tgctctccga ggaggtgcgc cagcgacttc aggctttccg ccaggacacc tacctgcaga 900tagctgcctt cactcgcgcc atcgaccagg agactgagga ggtccagcag cagctggcgc 960cacctccacc aggccacagt gccttcgccc cagagtttca acaaacagac agtggcaagg 1020ttctgagcaa gctgcaggcc cgtctggatg acctgtggga agacatcact cacagccttc 1080atgaccaggg ccacagccat ctgggggacc cctgaggatc tacctgccca ggcccattcc 1140cagctccttg tctggggagc cttggctctg agcctctagc atggttcagt ccttgaaagt 1200ggcctgttgg gtggagggtg gaaggtcctg tgcaggacag ggaggccacc aaaggggctg 1260ctgtctcctg catatccagc ctcctgcgac tccccaatct ggatgcatta cattcaccag 1320gctttgcaaa aaaa 133421879DNAHomo sapiens 2gagcagagca gataatggca agcatggctg ccgtgctcac ctgggctctg gctcttcttt 60cagcgttttc ggccacccag gcacggaaag gcttctggga ctacttcagc cagaccagcg 120gggacaaagg cagggtggag cagatccatc agcagaagat ggctcgcgag cccgcgaccc 180tgaaagacag ccttgagcaa gacctcaaca atatgaacaa gttcctggaa aagctgaggc 240ctctgagtgg gagcgaggct cctcggctcc cacaggaccc ggtgggcatg cggcggcagc 300tgcaggagga gttggaggag gtgaaggctc gcctccagcc ctacatggca gaggcgcacg 360agctggtggg ctggaatttg gagggcttgc ggcagcaact gaagccctac acgatggatc 420tgatggagca ggtggccctg cgcgtgcagg agctgcagga gcagttgcgc gtggtggggg 480aagacaccaa ggcccagttg ctggggggcg tggacgaggc ttgggctttg ctgcagggac 540tgcagagccg cgtggtgcac cacaccggcc gcttcaaaga gctcttccac ccatacgccg 600agagcctggt gagcggcatc gggcgccacg tgcaggagct gcaccgcagt gtggctccgc 660acgcccccgc cagccccgcg cgcctcagtc gctgcgtgca ggtgctctcc cggaagctca 720cgctcaaggc caaggccctg cacgcacgca tccagcagaa cctggaccag ctgcgcgaag 780agctcagcag agcctttgca ggcactggga ctgaggaagg ggccggcccg gacccccaga 840tgctctccga ggaggtgcgc cagcgacttc aggctttccg ccaggacacc tacctgcaga 900tagctgcctt cactcgcgcc atcgaccagg agactgagga ggtccagcag cagctggcgc 960cacctccacc aggccacagt gccttcgccc cagagtttca acaaacagac agtggcaagg 1020ttctgagcaa gctgcaggcc cgtctggatg acctgtggga agacatcact cacagccttc 1080atgaccaggg ccacagccat ctgggggacc cctgaggatc tacctgccca ggcccattcc 1140cagctccttg tctggggagc cttggctctg agcctctagc atggttcagt ccttgaaagt 1200ggcctgttgg gtggagggtg gaaggtcctg tgcaggacag ggaggccacc aaaggggctg 1260ctgtctcctg catatccagc ctcctgcgac tccccaatct ggatgcatta cattcaccag 1320gctttgcaaa cccagcctcc cagtgctcat ttgggaatgc tcatgagtta ctccattcaa 1380gggtgaggga gtagggaggg agaggcacca tgcatgtggg tgattatctg caagcctgtt 1440tgccgtgatg ctggaagcct gtgccactac atcctggagt ttggctctag tcacttctgg 1500ctgcctggtg gccactgcta cagctggtcc acagagagga gcacttgtct ccccagggct 1560gccatggcag ctatcagggg aatagaaggg agaaagagaa tatcatgggg agaacatgtg 1620atggtgtgtg aatatccctg ctggctctga tgctggtggg tacgaaaggt gtgggctgtg 1680ataggagagg gcagagccca tgtttcctga catagctcta cacctaaata agggactgaa 1740ccctcccaac tgtgggagct ccttaaaccc tctggggagc atactgtgtg ctctccccat 1800ctccagcccc tccctctggg ttcccaagtt gaagcctaga cttctggctc aaatgaaata 1860gatgtttatg ataaaaaaa 1879316000DNAHomo sapiensvariation(14695)..(14695)SNP1 polymorphic T or C 3ctcaagtaat ccttctgcct cagcctcctg agtagctagg accacaggtg ccaccatacc 60tggctaattt ttgtattctt tgcagagaca gggtctcgct acattgccca ggctggtatc 120gaactcctgg gctcaagcga tcctcctgcc ttgccttctc aaagtgttgg gattacaggc 180atgagccact gtgcccagcc tcaaaattta atgtataaag ttttccttaa tttttcttag 240cacaaaaacc ctggccccca acaataccta gttttctcca ggccggagtc ccactctttt 300acccttttca gagagaataa gcatctggtt ttctgctgct ttgggggtac ccagccaagt 360agagttgaag agaacagctg cttctcaaac agactctcga ccaactgcca tatttctagt 420cccactgcca cccactcttc cagaagaatg ttgacactaa tgtcagagca tttggagagt 480ttagtagtga aaatcagggg ccttcttggc tttctccact gctgcttcaa aattcatgtc 540aggtgtgcct gtcaccaccg tttgacyatt tggaagcttt ccagcttccc aaatgttgtt 600atttttgtct ccttttctat tttccctttg ggtttatgca ttttgtaaaa agtgcacttc 660aatgccacgt tattgagatt tcagagaaca gcagaggcta atgcatgcaa ttaatccacc 720gtccgttact agaagtcaat cggatgctct ttagtctctc ttccccatat actagtttaa 780aagttatcca ttctttctat tcgttttatg ggttatcctt aaaattttaa tattcttgtc 840tgacctaaca aagtctatag ataatcaata tccctatctt tctcccgaat aatgcaaagg 900ctgctgaatg ctttcacttt gatctctcct ttcccatttc caggttgctt cggtctgata 960ttttagttcc tcattacttt taacacctcc tccaaagtag tcccttcatc aatagatgtt 1020tttgagccct ccctaccatg tgataagcac tggtctaggc actgggagta cagtaggaaa 1080tgagataaac ttggccaggt gtagggtggc ttacacctgt aatcccaaca cttttgaggc 1140cgaggcgggc agctcgcttg agcccatgag ttcgagacca gcctgggaaa catagcgaga 1200cccccgtccc tacaaaaaaa tataaaaatt agctgggcat ggtggtgtat gcctgtggtc 1260ctagctactc agaagactga ggtgggagga tcatctgagc ccagggtggt cgaggctgca 1320gtgattacac cactgcactc catcctgggc aacggtgaga ccctgtctca aaaaacaaac 1380aaacaaacaa gcaaacaaaa cccccacaaa ctaaactatg tgtaaataca tttttgttag 1440gtagaactat atgaaattgc cactatttga ccaattttta gtgaaaacta gtctcataag 1500tgtgtgtgtg tgtattttca ctaatgtttt ttggatttac ctaaacgttt actaatttca 1560ttgctcccca tgtctccttc tatcctattc cttttttctg ggttctgttt ccttttcaga 1620tttttagtag ttcttttcag tgaggatctg tgagtggtaa actctctttc tctgaaatta 1680acttcttcct ctcaaataat agttcacctg agtataagtc ttggttggcc attaatttcc 1740tttcagtctt tagaaggtac attgatgata aatcagttgc cggtttaatc atgcttcgtg 1800tgtagatcat tagtctttct ctttggttga ttttaagata tccattgcct tcagtgttct 1860gcagattcct gtgatgtgtc ctcatttggt tgtgtgttaa tttttcctac caagactcag 1920gatgcttcct gtacctgagg attccggtca catcttgctt caatgtttga aatttctcag 1980ccatcatctt ttgaatattg cctcttccac agtccctgtg ttctcttcgt ggaaatccta 2040caggcatata ttggacctcc cattctgtcc tccatgtctc ttaccgtcta ttcataccct 2100cctttttata tttaattttt ttgagacaga gttttgctct gtggcccagg acggagtgca 2160attgcatgat cttggctcac ggcaaactct gcctcccagg ttcaagctat tctcctgcct 2220cagtctccca agtagctggg attacaggca tgcaccacca cgcccggcta atttttgtat 2280ttttagtaga gacggggttt caccatattg gccaggctgg tctgaaactc ctgacctcag 2340gtgatccacc cacctcggcc tcccaaagtg ctgggattat aggcatgagc caccatgcct 2400ggccaatatc acccgcctcg gcctcccaaa gtgctgggat tacaggcatg agccaccgtg 2460cctggtcaat atcctccttt ttatttctgt gattctttct gtgtgatttt ctcagatcta 2520ccttctagct tactaattct ctctccaact gtagctaaat gtgttttata ttataatgac 2580tatatttttt tcacttatag atgttctatt taattctctt tctttttgac aaatagaact 2640tatttcaaaa caaacaaaag gccaggcatg gtggttcatg cctgtaatcc cagtgctttg 2700ggaggctgag gcaggaggat tgcttgagcc caggagttca aggccagcct gggcaacata 2760gtgagatcct ttctctacca aaaaaataaa aaatcagctg ggtgttgtgg gacactcctg 2820tagtcccaga aactacagat tcttgggagg cagaggctgg aggattgctt gaggcagagg 2880ctggaggatt gcttgagcct gggaggttga ggctgcagtg aactgtgatc atgccactgc 2940actctagcct gggtgacaaa gtgagatcct gtctcaaaaa caaataaaca aacaagcaaa 3000gaaacaaaaa aatgcttaca caggttacta ctttcttgct gggatagttt taacactgcg 3060ttaagcataa acacctttct ttctgaaatg tatttgagat gtatattgat ttttaaaaaa 3120cccacacctc cattaaggtc tggtgatagc agtagaaaca atgtagagtg gctccacaat 3180catatagatg tttttggtgc gttctgagat ggagtccagg aacaccaagt aaagactgct 3240acctcacagt ttacatctga gttcttagaa gacaagactg aaggagaaca atttgtaaca 3300agatttactt ggcccgggtg tggtggctca cacctgtaat cccagtactt tgggagcttt 3360gggagtccga ggtgggtgga tcacctgagg tcaggagttc aagaccagcc tggccaacat 3420ggaataaccc ccatctctac taaaaataca aaaattagcc aggcacggtg gcacacgcct 3480gtaatcccag ctactcagga ggctgaggca ggagaatcgc ttgaacccaa gtgactgggt 3540tgccgagagc cgagatcacg ccactgtact ccagccaccc tgggtgacag agtgagactc 3600tgccaaaaaa aaaaaaaaaa aaaaaaaaat acttactgtt cagaaggaga agtcataatg 3660ttgctttaaa gaacaggtca caaaagaaag actctagaag atcttctcac ttggtgcata 3720tcaagtgtct atttgagacc catacacttg cttaatccat gtgtttaagg caaaagtgct 3780gctgctgagc agtaaggaat aaggtacctg ctaaccttta ccaatctaca ttttaaaatc 3840cttcttacta cacatccaga atgagtcagc aattcttgtg tattaaaaaa caaaaacaca 3900aaacaaagta gaggggcaac tctcttaaaa atgcagctat ccgcaaacac tgtgatacaa 3960aacgacagtc aaggaaaggg cagcacaaac aagttcacct ggaaggaatc tgttcaaagt 4020ctctggattt aagaacaagt tccctaaaag ctcttactta cagaagaaat cggataataa 4080atgtagctgg aatgatggaa ttctttaagt tttcattttg ttttgggcaa ctctgtggcc 4140caggctggag tgcagtggct tgatcacggc tcactgcagc ctcaacctcc caggctctgg 4200tgatcctccc acctcagcct cctgagtggc tgggactaca agcatgtgtg ccaccatgct 4260aggctaattt ttgtatttta tttttagttt tttttttttt tttttttttt ttgtagagac 4320aggttttgcc atgttgccaa ggctggtctc aaattcctgg gttcaagtga tcctcccatc 4380tcagccttcc aaagagctgg gattacaggc atgagctact gcacctggcc tagaattttt 4440taaaaatcac tatctggcaa ctctcaggat aatattcgat tcaggcaagg atcatcaatg 4500aatgctaaaa ccattgggtg aaaaattgtt gcagaatggg atgctcacat ggcttcaaag 4560tattgctcca caaattactt atcaataacg taaaaaacca aactttacta gccatggaga 4620aatctggttg ttatcacttt aatggagtga tcaaacttaa catcactaaa tagagtgcaa 4680cctccagcta ggatacagta agaagggcca gatatcacct agtatttttg ccaaaaatgt 4740ttaaccttaa tctaatcatg agaaagtaat cactcaaatc cagaatgtgg gacattttac 4800aagatgtcct ccttgcactc ttccaaaaaa aaatcaatgt catgaaaaca aacaaatggt 4860ggggttgggg agaacggttc taaatttaaa aactaaagtg ggataacaac cagatgagat 4920gtgttagagc ttgaatttac agagagagaa aaacaactat gaaagcattt tggggaaaat 4980ctgaatatgt aggatatgtt agatgatatt aaggaattgt gttaattttc aaaggtatga 5040taatgttttt ttcttttgta aaagagtcct tatttttcac aatgtatgtt gaagtattca 5100gagtgaagtg tcatgttggc tataattatt tcaaatggtt ccacacacaa agcacatacc 5160acatacacat atacatatac ctccaaccaa ctcaaaacat gttcaacact gaaactataa 5220gatgccacca aacagggaag catgagtgtg tgttgcatct acccattgta tcaatccagg 5280ttcagtcaga aaaacaaaag ccattccacg tatttcaagc atgaaaggct ttaaaacaaa 5340aaattaaagg tttatccaac tcttggaagg gctggaggag tgaccacctt ggtttgcagt 5400tcagaaggag tgactctcaa acgctcatta gtaagtggct acaaatggga agctcgcctt 5460attatgcctg caatatcaat gcatgtgatt cctgggaagg tcacccagaa gctgctttaa 5520actccaagcc tgtccatgct tctgtctgca accggcattg aaacataatg gcctctcctc 5580ttccgtctca cgctggctga ctctaaccta ggctcatata gagaagggat tctagaaaat 5640gtattaatag ttccaagtgt cccctctgca tctcataaaa gaccttagaa agggcactga 5700taatgctatt tgcaaaaaga caatccagcg cagttgtatt ttacagcaca ggctctttaa 5760gtttgggtta tcagcaaaaa accattagag tatgagaaat tcctttttaa attgtggcaa 5820aatatacata acataaaaat taccattgta gctattgtac attgtagcta agtatatagc 5880ccagtagcac taaatacatt tacactgttg tgcaccacta tctagctcca gaaactttta 5940atcttcccaa acagaaactt gtacctatta accaatacct tctccttcct cacttctcct 6000agaaaccaga attatacttt ctgtctctac aaatctgact attctggata cctcagaatc 6060acagtatgtg tcgttctacg actggcttgt ttcacttagc atcatgtctt caagggtcat 6120ccacgttata gcatgtgtca gatttccttt tcttttcttt tctttttttt tttttttgag 6180acacagtctc gctctgtcac ccaggctgga gggcagtagc acaatctcag ttcactgcag 6240cctctgcctt ccaggttcaa gcaattctcg tgcctcagcc tcccaagtag ctggaatgac 6300aggcatgcac caccacacct ggctaatttt tgtattttta gtagagacgg ggttttacca 6360tattggccag gctggtcttg cacttccggc ctcaagtgat ctgcccgtct cggcctccca 6420aagtgctggg attacaggcg tgagccacta tgcctggccc ccgattgtca ttatttaagg 6480ctaagtgata ttttgttgtc tgtatatacc acaatttgtt tattcattca tctgtcaatg 6540gacatttggg ttgtttccac cccctggtta ttgtggataa tactactagg aacacgagca 6600tacaaatatc tgctccagtc cctgctttta tcttttggat atatgcccag aggtggaatt 6660gctgggtcat aaggtaattc tagattaaat tttttgaggg actgccgtat tgttctccac 6720catagctgca ccattttacc tttccagcag cagcgtacaa gcggtccagc ttctccacat 6780cttcacccac acttgctatt tttggctttt attttatttt ttaaaataac attctaatgg 6840gtgttaagtg gtcagaaatg gttcttttag gagtagagat agaggccagg gggatggctc 6900acacctgtaa tcccagcact ttgggaggcc taggtgggcg gatcacttgg ggtcaggagt 6960ttgagatcag cctggccaac atggtgaaac tccatctcta ttaaaaatac aaaaattcgc 7020tgggtgtggt ggtgtgcact cccagctact tgggaggctg agggaagaga atcgcttgaa 7080cccgggaggc ggtagttgca gtgagccgag atcacaccac tgtatggcct ggtgacagag 7140caaggctctg tctcaaaaaa aaaaaaaaaa aaaaaagagt agagatagaa aagcattgaa 7200aacacagcct cagctcagct cagtctgcca tggtgggaag ccattaattc ttcactcttg 7260aaaccttttc gtccttggtg tggcagaggc tgcaagtctc ctctgcaact ttattcttcc 7320cttctttctc agttataaaa tccctgattt tagaaatatc tttattgaga tataattcac 7380ataccataac attcactaca attgaatggt ttttagtata ttcacagatt tgtacaacta 7440tcaccacaaa ctaagtttag aacttttttc atcatcccac aaagaaaccc cacacccatt 7500agcagttatt cactatttct ccccaatcaa cctcccctcc cctcaatagc cctaggcagc 7560caccagtcta ctttctgtct ctacctattt gtcttttctg gacattttat acaaatgaga 7620ttttacaaca tgtagtcttt tgtgactggc ttttttcccc tagcataatg ttttccaggt 7680tcatctgtgg tgtagcaggt atcagtactt caaccctttt tattgccaaa taatattcca 7740ctatatggat aggtaacatt ttgtttatcc attcatcaat tgatggacat ttgggttgtt 7800tccattttct tgactgttat gaataatgtt gccatgaaca ttaatgcaca agtttttgtg 7860cagatgtgta ttttcatgtg tcttggtttt atacctagga atagaattgc tgagtcatag 7920gagaactcct ccatgtttaa ccattaatga actgccgaac tgttttccaa agaaattgca 7980ccattgtaca atcccaccag caatgtatga gggtagaatc cctgattttt aactgatcat 8040tgaactcagg cccattcaaa acaaagatga catttcctaa ccttccttac aagtagttct 8100gaccagtgag atgggagaag aagttaggtt ttgtccttaa aagaaaggag agtggctggg 8160tgcagtggct cacgcctgta atcccagcac tttgggaggc cgaggtgggc ggatcacctg 8220aggccgggag ttcaagacta gcctgaccaa catggagaaa cctcgtctct actaaaaata 8280caaaattagc tgggcgtcgt ggcgcatgcc tgtaatccca gctactcagg aggctgaggc 8340aggagaatcg cttgaacctg ggaggtggag gttgtgatga gccgagattg tgccatcgca 8400ctctagcctg ggcaagaaga gcgaaactcc atctcaaaaa aaaaaagaaa gaaaggagag 8460tacattctac actctcctct cctccaccct gccccctttc cagtggctgg atgtggacat 8520ggtggtaagc tatcttagat catgtggaca agggaaacac atagggatat tagaacctcc 8580agacagaagg aacttaggac cctggacagc tttgtggagc agtgctccca taccagcgtg 8640aagttttgta tgggagaaaa catacatttc cagcttttgt aagccactgt tattttgggt 8700ctctctcaga gcagccaaat atataattta actaatatat tttctttctg tgattcttct 8760ttattttgat tatacttcta cttctctgcc cctcttttag gtgggaggtg ctgctccaag 8820cactaactca gaatatagac cctctccctc ttgtaatagt gccagcttgg agttctttgc 8880ttccactgta ggggaaggaa ggaaaaaata tggagaactc acatccactc ttcattgtcc 8940tcaaacagaa gtaacccatt ttgttctgct cacagcccat tggccagaac taactgtatg 9000gccccaatct aattgcaaag gagtctggga agtacagcac agcacatgga tctttggaaa 9060gcgttaattt tctctgccaa aggcttcttt tgttgttgtt gttgtttgaa atggagtccc 9120gctccgtcac ccgggctgga gtgcagtggc cctatctcag ctcactgcaa cctccacctc 9180ccaggttcaa gcactcctcc agccttggcc tcccaagtag ctaggattac aggcgtgtgc 9240catcatacct ggctattttt tttgtatttt tagtagagac agggttttac cgtgttggcc 9300aggctggtct caaactcttg gcttcaagtg atccgcctgc ttcggtctcc caaagtgttg 9360ggattacagg cgtgagccac tgtacccggc caatactggt gttttctgac ctcagcgttt 9420ttcttttctc ctgccatgta ctctccctag agatttcatc tactcccaag tcttccactg 9480ctcctatctg ctgatgactc ccaaaactca gtctccagcc gagacttctc tcctgggctt 9540gagacatatg tatccaactg ccagaacatc tccagtggac agcctttggg cacaaggcca 9600cactagcttg tgggtacaag taatcaccca aagtcaattt cagtggctct ccactcccac 9660attttttcaa cccctggaaa tgttcccttc ccaaatactc tgagtctctc ttctcttttg 9720atgactgtgg ttttgtgact ggatggtagc tcctgttgct ttttttcctt tcaaatgaat 9780tttctcttag gaggcttctt agccattaag caaatagacc tcaactgggc ttgccctatg 9840cctatctgaa acccagctta ggttcgagtt aggactcctg ttaatctgag ctctcacttc 9900ctgtcccaac ctgcttattt tttttggtga aagaaaatat catcccccta gttgctcaga 9960aacctgggaa tcattgggat tttttcctct ccttcacctt cctcatccaa tcagtcacca 10020agtgctatca actctgctgc cttagtagcc ctcaatatat acttacctat caaccatcac 10080tgctatgcca tacttcaggc cttcatttct cacttggatt attaaaatat ccctaaatag 10140ttcctctgcc ttctctctgg ccaccctcct gagtgatctc tccatcatgt acctttcagt 10200gactgcttgc acaagcccct ttgtgacttg gtcatagtct gctctcttga accaccagag 10260ccaagcacct gggttctgat tctggttgca actcttactg cgtgatgatg gacaggccac 10320ttgatctcct caaacctcag ttcctaaatc aaatgaatga ttgaattcag tcacttaact 10380ttgtatgtag taggtaggca ctgtgcaaaa catactagtg gatatagaga tgaataagaa 10440aaagcccctg cactcaaaga gctctcggat tcatcaacaa attattgtgc agttagatag 10500taagtgctat aatccaggaa tatacagtgt tgtgtgaata atgtggaatc agtttatctc 10560cagagcagaa aaaggtgaag gccgaagaag gcattcagag tgatactgga gctgtgtagc 10620aggggctact actgttccct caaatccttt cttctcttct tcctgagtaa tagagtcctt 10680tttcagctag gcatacagcc atccaaaata aaaactatat ttcccagcct ccttttcagc 10740taagtgtagc catgtgacta agttgtggcc aatgggatgt cagtacaagt ggtaatggca 10800atatctggga tgtgtcttta aaaggaagga acatgtcctt ctccttttcc tccttcctgt 10860tccctgggag gtgaacttgg tagctggagg tgaagcagct ggaacttgga tatgagcgtc 10920ttgttgatga taatagtgca acaagataaa agcagcccga gctggcctac atttacatga 10980gaaggaaata agactccatt ttgtttaagc tattctcttg catatatata tacacatata 11040tatcagctaa gtgtagccat gtgactaact tgtggccaat gggatgtcag tatgagtggt 11100aagagcagta tcttgtatat atatatgtgt atatatacac acacacatat atatacatat 11160atgtatgcaa gaatataaaa tatacatgtg tgtgtgtata tatatacaca catatatatg 11220tcccttgcag ccaagtctaa ttctaactaa tacaaactaa ctctaaaaaa tgaatatata 11280ttcaccaggg gataggctat ttcaagcaga gggaagcctg tataaaggct cagggaatgc 11340tgtggtttta tgtggcagca gatgagactg gaaatgagtc aggatgagcc acagtggagg 11400atgaattaaa tgggcaggag tgtggtagaa agacctgttg gaggctatga atgcaatcaa 11460ggtgacagac aactggtgca atgatggtag tggaaatgga ggagagggga ttgattcaag 11520atgcatttag gaccaagaat cgggagcttg tgaacgtgtg tatgagtact gtagacggag 11580tgggtgtgtc atcagagaag atctgagcat ttgggcttgc tctcctcaga ggccctgcga 11640gtggagttca

gcttttcctc atggggcaaa tctyactttc gctccagttc ctggggctca 11700gagtccctgg cccagatgcc tcttgccatc tcatcttcac cctgcctggc ttcccttgct 11760tgttccagga ttgtttcata aagagggatg tggttggtct ttaaccctat gaatgctggc 11820tgaggatgcc tgcggaacct gtagtgaagc tttcaggggc tgctcgggtt ctggctggta 11880ggtgaacact gtccatcttg ccggctggga cacagtgact ctgggtagtt gtgtaagaga 11940ggggcccttg gcagacaaac aggttcttct ctgttggtgg gccagccagc aggtcagtgg 12000gaaggttaaa ggtcatgggg tttgggagaa actgggtgag gagttcagcc ccatcccccg 12060taaagctcct gggaagcact tctctactgg ggcagcccct gataccaggg cactcattaa 12120ccctctgggt gccagggaaa gggcaggagg tgagtgctgg gaggcagctg aggtcaactt 12180cttttgaact tccacgtggt atttactcag agcaattggt gccagaggct cagggccctg 12240gagtataaag cagaatgtct gctctctgtg cccagacgtg agcaggtgag cagctggggc 12300agagggatgg gggtcacagt cctaagggag ggcattgcag gtggcctcag gggagagcct 12360ggggtggccc ctaagacgtc ctcttggaac attttggcag agttgcctct tcgccctcat 12420tatggctcag tttttccacc atgaaatggg agggagggag acaggtgggc aggggagagg 12480tggtagaagt ggcctagaga actgttcctg gggtctggga cctttgcgaa ggggttagag 12540caccacgctc cctgctatgt gactgaggta gcaagagcac gccctcttcc catgtttgag 12600gaagacaccc tagcctcctt gactcaccta ggtcagtcct cttgagcccc aacagctctg 12660tgctccccag cccaaggaag gggtaacagg atttcgggca gttgcccctg cagaggcccc 12720ctgggcaagt cccctgcgcc atgtcccttc gtctccttct tcccctaacc aggcctccct 12780ccacctgtct tctcagagca grta atg gca agc atg gct gcc gtg ctc acc 12831Met Ala Ser Met Ala Ala Val Leu Thr1 5tgg gct ctg gct ctt ctt tca g gtgggtctcc gaccctgact tcaacgtggg 12883Trp Ala Leu Ala Leu Leu Ser10 15ggtgtgggtg gaggctggcc agagggccct gtccaccctg ggggaggaga gcccaggccc 12943tgattaccta gtccctctcc acag cg ttt tsg gcc acc cag gca cgg aaa 12993Ala Phe Xaa Ala Thr Gln Ala Arg Lys 20 25ggc ttc tgg gac tac ttc agc cag acc agc ggg gac aaa ggc agg gtg 13041Gly Phe Trp Asp Tyr Phe Ser Gln Thr Ser Gly Asp Lys Gly Arg Val 30 35 40gag cag atc cat cag cag aag atg gct cgc gag ccc gc gtgagtgccc 13089Glu Gln Ile His Gln Gln Lys Met Ala Arg Glu Pro Ala 45 50aggggaaggg gtgtaggcga agggaggaga cagctgggcc atgccatgat gacctgcctc 13149tgctgcctca acctctgtgg ccgctgctgg gacagaggaa aggagcggtg ctagctctgt 13209ctgcagatcc cggccatcct gggctcttta gcgccctctg cctgcagccc ccgccttgac 13269aactccgtag ctgttgcccc cttgctcact gaggcgcggg acctgggatc aatcgggagg 13329acgcccgctg cagtccccag aatcaaagga tgatgtggcg catctatgtt tctttggaga 13389gtgttgtagg tctggatttg tatgggcaat gtgtttgtgc ttcgtgcgtg agttgttact 13449ggccagggct aggacaagag ccctcgaccc tggggccaac gccctgcgtc cttggttccc 13509ccagaggatc agtgcgcgat gacttgggga caaaggagat gatggrggct agcagtctga 13569cggcctggat atctgtcccc ttctccag g acc ctg aaa gac agc ctt gag caa 13622Thr Leu Lys Asp Ser Leu Glu Gln55 60gac ctc aac aat atg aac aag ttc ctg gaa aag ctg agg cct ctg agt 13670Asp Leu Asn Asn Met Asn Lys Phe Leu Glu Lys Leu Arg Pro Leu Ser 65 70 75ggg agc gag gct cct cgg ctc cca cag gac ccg gtg ggc atg cgg cgg 13718Gly Ser Glu Ala Pro Arg Leu Pro Gln Asp Pro Val Gly Met Arg Arg 80 85 90cag ctg cag gag gag ttg gag gag gtg aag gct cgc ctc cag ccc tac 13766Gln Leu Gln Glu Glu Leu Glu Glu Val Lys Ala Arg Leu Gln Pro Tyr95 100 105 110atg gca gag gcg cac gag ctg gtg ggc tgg aat ttg gag ggc ttg cgg 13814Met Ala Glu Ala His Glu Leu Val Gly Trp Asn Leu Glu Gly Leu Arg 115 120 125cag caa ctg aag ccc tac acg atg gat ctg atg gag cag gtg gcc ctg 13862Gln Gln Leu Lys Pro Tyr Thr Met Asp Leu Met Glu Gln Val Ala Leu 130 135 140cgc gtg cag gag ctg cag gag cag ttg cgc gtg gtg ggg gaa gac acc 13910Arg Val Gln Glu Leu Gln Glu Gln Leu Arg Val Val Gly Glu Asp Thr 145 150 155aag gcc cag ttg ctg ggg ggc gtg gac gag gct tgg gct ttg ctg cag 13958Lys Ala Gln Leu Leu Gly Gly Val Asp Glu Ala Trp Ala Leu Leu Gln 160 165 170gga ctg cag agc cgc gtg gtg cac cac acc ggc cgc ttc aaa gag ctc 14006Gly Leu Gln Ser Arg Val Val His His Thr Gly Arg Phe Lys Glu Leu175 180 185 190ttc cac cca tac gcc gag agc ctg gtg agc ggc atc ggg cgc cac gtg 14054Phe His Pro Tyr Ala Glu Ser Leu Val Ser Gly Ile Gly Arg His Val 195 200 205cag gag ctg cac cgc agt gtg gct ccg cac gcc ccc gcc agc ccc gcg 14102Gln Glu Leu His Arg Ser Val Ala Pro His Ala Pro Ala Ser Pro Ala 210 215 220cgc ctc agt cgc tgc gtg cag gtg ctc tcc cgg aag ctc acg ctc aag 14150Arg Leu Ser Arg Cys Val Gln Val Leu Ser Arg Lys Leu Thr Leu Lys 225 230 235gcc aag gcc ctg cac gca cgc atc cag cag aac ctg gac cag ctg cgc 14198Ala Lys Ala Leu His Ala Arg Ile Gln Gln Asn Leu Asp Gln Leu Arg 240 245 250gaa gag ctc agc aga gcc ttt gca ggc act ggg act gag gaa ggg gcc 14246Glu Glu Leu Ser Arg Ala Phe Ala Gly Thr Gly Thr Glu Glu Gly Ala255 260 265 270ggc ccg gac ccc cag atg ctc tcc gag gag gtg cgc cag cga ctt cag 14294Gly Pro Asp Pro Gln Met Leu Ser Glu Glu Val Arg Gln Arg Leu Gln 275 280 285gct ttc cgc cag gac acc tac ctg cag ata gct gcc ttc act cgc gcc 14342Ala Phe Arg Gln Asp Thr Tyr Leu Gln Ile Ala Ala Phe Thr Arg Ala 290 295 300atc gac cag gag act gag gag gtc cag cag cag ctg gcg cca cct cca 14390Ile Asp Gln Glu Thr Glu Glu Val Gln Gln Gln Leu Ala Pro Pro Pro 305 310 315cca ggc cac agt gcc ttc gcc cca gag ttt caa caa aca gac agt ggc 14438Pro Gly His Ser Ala Phe Ala Pro Glu Phe Gln Gln Thr Asp Ser Gly 320 325 330aag gtt ctg agc aag ctg cag gcc cgt ctg gat gac ctg tgg gaa gac 14486Lys Val Leu Ser Lys Leu Gln Ala Arg Leu Asp Asp Leu Trp Glu Asp335 340 345 350atc act cac agc ctt cat gac cag ggc cac agc cat ctg ggg gac ccc 14534Ile Thr His Ser Leu His Asp Gln Gly His Ser His Leu Gly Asp Pro 355 360 365tga ggatctacct gcccaggccc attcccagct ccttgtctgg ggagccttgg 14587ctctgagcct ctagcatggt tcagtccttg aaagtggcct gttgggtgga gggtggaagg 14647tcctgtgcag gacagggagg ccaccaaagg ggctgctgtc tcctgcayat ccagcctcct 14707gcgactcccc aatctggatg cattacattc accaggcttt gcaaacccag cctcccagtg 14767ctcatttggg aatgctcatg agttactcca ttcaagggtg agggagtagg gagggagagg 14827caccatgcat gtgggtgatt atctgcaagc ctgtttgccg tgatgctgga agcctgtgcc 14887actacatcct ggagtttggc tctagtcact tctggctgcc tggtggccac tgctacagct 14947ggtccacaga gaggagcact tgtctcccca gggctgccat ggcagctatc aggggaatag 15007aagggagaaa gagaatatca tggggagaac atgtgatggt gtgtgaatat ccctgctggc 15067tctgatgctg gtgggtacga aaggtgtggg ctgtgatagg agagggcaga gcccatgttt 15127cctgacatag ctctacacct aaataaggga ctgaaccctc ccaactgtgg gagctcctta 15187aaccctctgg ggagcatact gtgtgctctc cccatctcca gcccctccct ctgggttccc 15247aagttgaagc ctagacttct ggctcaaatg aaatagatgt ttatgataga agtttgcctg 15307gcgtgactct catttggacc atgtctgaaa gcagtggcct caccactatc cccaaagcac 15367acccatcacc cactccattc ccttgctgct ctttctcatc cacccactcc cagtccaggt 15427ctgtcaaagg gggtctggct gggctctgct tcagggatcc tggctagaca acggctgtct 15487gtcacacctg gcaggagggc ctgggttacg ggcccttcct ctgcacctgc actgttcact 15547agcctgctcc cccacaggac actgtgcatg gaatgcaggc tgtgtctgga agagctgtgg 15607ccctggtgga cctaagattc ctgaggtggg ctgcctcctt tgttcctgct gttctagagt 15667ttgaatggcc tctttttatg ccggactctc ttctggggac tcccctcact caggggcacc 15727aatgctccct atagatcccc tgggaactga aactggggtg tggtggagga cgtggaaagg 15787gtaaacacag ctccttgtct ttggacttcc ctgtccggcc ccctttcctc ccagctcagc 15847ctactgtccc cgggttctca gcacctgcct gctccccaac cccatagcac agaccccaca 15907catatgtagg ctcatcatgc ctgcaggctg gtcttccctg acaccgtgga ttttgacaat 15967gttggcaaca gaactgggtt gtggacccag cac 16000416000DNAhomo sapiens 4ctcaagtaat ccttctgcct cagcctcctg agtagctagg accacaggtg ccaccatacc 60tggctaattt ttgtattctt tgcagagaca gggtctcgct acattgccca ggctggtatc 120gaactcctgg gctcaagcga tcctcctgcc ttgccttctc aaagtgttgg gattacaggc 180atgagccact gtgcccagcc tcaaaattta atgtataaag ttttccttaa tttttcttag 240cacaaaaacc ctggccccca acaataccta gttttctcca ggccggagtc ccactctttt 300acccttttca gagagaataa gcatctggtt ttctgctgct ttgggggtac ccagccaagt 360agagttgaag agaacagctg cttctcaaac agactctcga ccaactgcca tatttctagt 420cccactgcca cccactcttc cagaagaatg ttgacactaa tgtcagagca tttggagagt 480ttagtagtga aaatcagggg ccttcttggc tttctccact gctgcttcaa aattcatgtc 540aggtgtgcct gtcaccaccg tttgaccatt tggaagcttt ccagcttccc aaatgttgtt 600atttttgtct ccttttctat tttccctttg ggtttatgca ttttgtaaaa agtgcacttc 660aatgccacgt tattgagatt tcagagaaca gcagaggcta atgcatgcaa ttaatccacc 720gtccgttact agaagtcaat cggatgctct ttagtctctc ttccccatat actagtttaa 780aagttatcca ttctttctat tcgttttatg ggttatcctt aaaattttaa tattcttgtc 840tgacctaaca aagtctatag ataatcaata tccctatctt tctcccgaat aatgcaaagg 900ctgctgaatg ctttcacttt gatctctcct ttcccatttc caggttgctt cggtctgata 960ttttagttcc tcattacttt taacacctcc tccaaagtag tcccttcatc aatagatgtt 1020tttgagccct ccctaccatg tgataagcac tggtctaggc actgggagta cagtaggaaa 1080tgagataaac ttggccaggt gtagggtggc ttacacctgt aatcccaaca cttttgaggc 1140cgaggcgggc agctcgcttg agcccatgag ttcgagacca gcctgggaaa catagcgaga 1200cccccgtccc tacaaaaaaa tataaaaatt agctgggcat ggtggtgtat gcctgtggtc 1260ctagctactc agaagactga ggtgggagga tcatctgagc ccagggtggt cgaggctgca 1320gtgattacac cactgcactc catcctgggc aacggtgaga ccctgtctca aaaaacaaac 1380aaacaaacaa gcaaacaaaa cccccacaaa ctaaactatg tgtaaataca tttttgttag 1440gtagaactat atgaaattgc cactatttga ccaattttta gtgaaaacta gtctcataag 1500tgtgtgtgtg tgtattttca ctaatgtttt ttggatttac ctaaacgttt actaatttca 1560ttgctcccca tgtctccttc tatcctattc cttttttctg ggttctgttt ccttttcaga 1620tttttagtag ttcttttcag tgaggatctg tgagtggtaa actctctttc tctgaaatta 1680acttcttcct ctcaaataat agttcacctg agtataagtc ttggttggcc attaatttcc 1740tttcagtctt tagaaggtac attgatgata aatcagttgc cggtttaatc atgcttcgtg 1800tgtagatcat tagtctttct ctttggttga ttttaagata tccattgcct tcagtgttct 1860gcagattcct gtgatgtgtc ctcatttggt tgtgtgttaa tttttcctac caagactcag 1920gatgcttcct gtacctgagg attccggtca catcttgctt caatgtttga aatttctcag 1980ccatcatctt ttgaatattg cctcttccac agtccctgtg ttctcttcgt ggaaatccta 2040caggcatata ttggacctcc cattctgtcc tccatgtctc ttaccgtcta ttcataccct 2100cctttttata tttaattttt ttgagacaga gttttgctct gtggcccagg acggagtgca 2160attgcatgat cttggctcac ggcaaactct gcctcccagg ttcaagctat tctcctgcct 2220cagtctccca agtagctggg attacaggca tgcaccacca cgcccggcta atttttgtat 2280ttttagtaga gacggggttt caccatattg gccaggctgg tctgaaactc ctgacctcag 2340gtgatccacc cacctcggcc tcccaaagtg ctgggattat aggcatgagc caccatgcct 2400ggccaatatc acccgcctcg gcctcccaaa gtgctgggat tacaggcatg agccaccgtg 2460cctggtcaat atcctccttt ttatttctgt gattctttct gtgtgatttt ctcagatcta 2520ccttctagct tactaattct ctctccaact gtagctaaat gtgttttata ttataatgac 2580tatatttttt tcacttatag atgttctatt taattctctt tctttttgac aaatagaact 2640tatttcaaaa caaacaaaag gccaggcatg gtggttcatg cctgtaatcc cagtgctttg 2700ggaggctgag gcaggaggat tgcttgagcc caggagttca aggccagcct gggcaacata 2760gtgagatcct ttctctacca aaaaaataaa aaatcagctg ggtgttgtgg gacactcctg 2820tagtcccaga aactacagat tcttgggagg cagaggctgg aggattgctt gaggcagagg 2880ctggaggatt gcttgagcct gggaggttga ggctgcagtg aactgtgatc atgccactgc 2940actctagcct gggtgacaaa gtgagatcct gtctcaaaaa caaataaaca aacaagcaaa 3000gaaacaaaaa aatgcttaca caggttacta ctttcttgct gggatagttt taacactgcg 3060ttaagcataa acacctttct ttctgaaatg tatttgagat gtatattgat ttttaaaaaa 3120cccacacctc cattaaggtc tggtgatagc agtagaaaca atgtagagtg gctccacaat 3180catatagatg tttttggtgc gttctgagat ggagtccagg aacaccaagt aaagactgct 3240acctcacagt ttacatctga gttcttagaa gacaagactg aaggagaaca atttgtaaca 3300agatttactt ggcccgggtg tggtggctca cacctgtaat cccagtactt tgggagcttt 3360gggagtccga ggtgggtgga tcacctgagg tcaggagttc aagaccagcc tggccaacat 3420ggaataaccc ccatctctac taaaaataca aaaattagcc aggcacggtg gcacacgcct 3480gtaatcccag ctactcagga ggctgaggca ggagaatcgc ttgaacccaa gtgactgggt 3540tgccgagagc cgagatcacg ccactgtact ccagccaccc tgggtgacag agtgagactc 3600tgccaaaaaa aaaaaaaaaa aaaaaaaaat acttactgtt cagaaggaga agtcataatg 3660ttgctttaaa gaacaggtca caaaagaaag actctagaag atcttctcac ttggtgcata 3720tcaagtgtct atttgagacc catacacttg cttaatccat gtgtttaagg caaaagtgct 3780gctgctgagc agtaaggaat aaggtacctg ctaaccttta ccaatctaca ttttaaaatc 3840cttcttacta cacatccaga atgagtcagc aattcttgtg tattaaaaaa caaaaacaca 3900aaacaaagta gaggggcaac tctcttaaaa atgcagctat ccgcaaacac tgtgatacaa 3960aacgacagtc aaggaaaggg cagcacaaac aagttcacct ggaaggaatc tgttcaaagt 4020ctctggattt aagaacaagt tccctaaaag ctcttactta cagaagaaat cggataataa 4080atgtagctgg aatgatggaa ttctttaagt tttcattttg ttttgggcaa ctctgtggcc 4140caggctggag tgcagtggct tgatcacggc tcactgcagc ctcaacctcc caggctctgg 4200tgatcctccc acctcagcct cctgagtggc tgggactaca agcatgtgtg ccaccatgct 4260aggctaattt ttgtatttta tttttagttt tttttttttt tttttttttt ttgtagagac 4320aggttttgcc atgttgccaa ggctggtctc aaattcctgg gttcaagtga tcctcccatc 4380tcagccttcc aaagagctgg gattacaggc atgagctact gcacctggcc tagaattttt 4440taaaaatcac tatctggcaa ctctcaggat aatattcgat tcaggcaagg atcatcaatg 4500aatgctaaaa ccattgggtg aaaaattgtt gcagaatggg atgctcacat ggcttcaaag 4560tattgctcca caaattactt atcaataacg taaaaaacca aactttacta gccatggaga 4620aatctggttg ttatcacttt aatggagtga tcaaacttaa catcactaaa tagagtgcaa 4680cctccagcta ggatacagta agaagggcca gatatcacct agtatttttg ccaaaaatgt 4740ttaaccttaa tctaatcatg agaaagtaat cactcaaatc cagaatgtgg gacattttac 4800aagatgtcct ccttgcactc ttccaaaaaa aaatcaatgt catgaaaaca aacaaatggt 4860ggggttgggg agaacggttc taaatttaaa aactaaagtg ggataacaac cagatgagat 4920gtgttagagc ttgaatttac agagagagaa aaacaactat gaaagcattt tggggaaaat 4980ctgaatatgt aggatatgtt agatgatatt aaggaattgt gttaattttc aaaggtatga 5040taatgttttt ttcttttgta aaagagtcct tatttttcac aatgtatgtt gaagtattca 5100gagtgaagtg tcatgttggc tataattatt tcaaatggtt ccacacacaa agcacatacc 5160acatacacat atacatatac ctccaaccaa ctcaaaacat gttcaacact gaaactataa 5220gatgccacca aacagggaag catgagtgtg tgttgcatct acccattgta tcaatccagg 5280ttcagtcaga aaaacaaaag ccattccacg tatttcaagc atgaaaggct ttaaaacaaa 5340aaattaaagg tttatccaac tcttggaagg gctggaggag tgaccacctt ggtttgcagt 5400tcagaaggag tgactctcaa acgctcatta gtaagtggct acaaatggga agctcgcctt 5460attatgcctg caatatcaat gcatgtgatt cctgggaagg tcacccagaa gctgctttaa 5520actccaagcc tgtccatgct tctgtctgca accggcattg aaacataatg gcctctcctc 5580ttccgtctca cgctggctga ctctaaccta ggctcatata gagaagggat tctagaaaat 5640gtattaatag ttccaagtgt cccctctgca tctcataaaa gaccttagaa agggcactga 5700taatgctatt tgcaaaaaga caatccagcg cagttgtatt ttacagcaca ggctctttaa 5760gtttgggtta tcagcaaaaa accattagag tatgagaaat tcctttttaa attgtggcaa 5820aatatacata acataaaaat taccattgta gctattgtac attgtagcta agtatatagc 5880ccagtagcac taaatacatt tacactgttg tgcaccacta tctagctcca gaaactttta 5940atcttcccaa acagaaactt gtacctatta accaatacct tctccttcct cacttctcct 6000agaaaccaga attatacttt ctgtctctac aaatctgact attctggata cctcagaatc 6060acagtatgtg tcgttctacg actggcttgt ttcacttagc atcatgtctt caagggtcat 6120ccacgttata gcatgtgtca gatttccttt tcttttcttt tctttttttt tttttttgag 6180acacagtctc gctctgtcac ccaggctgga gggcagtagc acaatctcag ttcactgcag 6240cctctgcctt ccaggttcaa gcaattctcg tgcctcagcc tcccaagtag ctggaatgac 6300aggcatgcac caccacacct ggctaatttt tgtattttta gtagagacgg ggttttacca 6360tattggccag gctggtcttg cacttccggc ctcaagtgat ctgcccgtct cggcctccca 6420aagtgctggg attacaggcg tgagccacta tgcctggccc ccgattgtca ttatttaagg 6480ctaagtgata ttttgttgtc tgtatatacc acaatttgtt tattcattca tctgtcaatg 6540gacatttggg ttgtttccac cccctggtta ttgtggataa tactactagg aacacgagca 6600tacaaatatc tgctccagtc cctgctttta tcttttggat atatgcccag aggtggaatt 6660gctgggtcat aaggtaattc tagattaaat tttttgaggg actgccgtat tgttctccac 6720catagctgca ccattttacc tttccagcag cagcgtacaa gcggtccagc ttctccacat 6780cttcacccac acttgctatt tttggctttt attttatttt ttaaaataac attctaatgg 6840gtgttaagtg gtcagaaatg gttcttttag gagtagagat agaggccagg gggatggctc 6900acacctgtaa tcccagcact ttgggaggcc taggtgggcg gatcacttgg ggtcaggagt 6960ttgagatcag cctggccaac atggtgaaac tccatctcta ttaaaaatac aaaaattcgc 7020tgggtgtggt ggtgtgcact cccagctact tgggaggctg agggaagaga atcgcttgaa 7080cccgggaggc ggtagttgca gtgagccgag atcacaccac tgtatggcct ggtgacagag 7140caaggctctg tctcaaaaaa aaaaaaaaaa aaaaaagagt agagatagaa aagcattgaa 7200aacacagcct cagctcagct cagtctgcca tggtgggaag ccattaattc ttcactcttg 7260aaaccttttc gtccttggtg tggcagaggc tgcaagtctc ctctgcaact ttattcttcc 7320cttctttctc agttataaaa tccctgattt tagaaatatc tttattgaga tataattcac 7380ataccataac attcactaca attgaatggt ttttagtata ttcacagatt tgtacaacta 7440tcaccacaaa ctaagtttag aacttttttc atcatcccac aaagaaaccc cacacccatt 7500agcagttatt cactatttct ccccaatcaa cctcccctcc cctcaatagc cctaggcagc 7560caccagtcta ctttctgtct ctacctattt gtcttttctg gacattttat acaaatgaga 7620ttttacaaca tgtagtcttt tgtgactggc ttttttcccc tagcataatg ttttccaggt 7680tcatctgtgg tgtagcaggt atcagtactt caaccctttt tattgccaaa taatattcca 7740ctatatggat aggtaacatt ttgtttatcc attcatcaat tgatggacat ttgggttgtt 7800tccattttct tgactgttat gaataatgtt gccatgaaca ttaatgcaca agtttttgtg 7860cagatgtgta ttttcatgtg tcttggtttt atacctagga atagaattgc tgagtcatag 7920gagaactcct ccatgtttaa ccattaatga actgccgaac tgttttccaa agaaattgca 7980ccattgtaca atcccaccag caatgtatga gggtagaatc cctgattttt aactgatcat 8040tgaactcagg cccattcaaa acaaagatga catttcctaa ccttccttac aagtagttct 8100gaccagtgag atgggagaag aagttaggtt ttgtccttaa aagaaaggag agtggctggg 8160tgcagtggct

cacgcctgta atcccagcac tttgggaggc cgaggtgggc ggatcacctg 8220aggccgggag ttcaagacta gcctgaccaa catggagaaa cctcgtctct actaaaaata 8280caaaattagc tgggcgtcgt ggcgcatgcc tgtaatccca gctactcagg aggctgaggc 8340aggagaatcg cttgaacctg ggaggtggag gttgtgatga gccgagattg tgccatcgca 8400ctctagcctg ggcaagaaga gcgaaactcc atctcaaaaa aaaaaagaaa gaaaggagag 8460tacattctac actctcctct cctccaccct gccccctttc cagtggctgg atgtggacat 8520ggtggtaagc tatcttagat catgtggaca agggaaacac atagggatat tagaacctcc 8580agacagaagg aacttaggac cctggacagc tttgtggagc agtgctccca taccagcgtg 8640aagttttgta tgggagaaaa catacatttc cagcttttgt aagccactgt tattttgggt 8700ctctctcaga gcagccaaat atataattta actaatatat tttctttctg tgattcttct 8760ttattttgat tatacttcta cttctctgcc cctcttttag gtgggaggtg ctgctccaag 8820cactaactca gaatatagac cctctccctc ttgtaatagt gccagcttgg agttctttgc 8880ttccactgta ggggaaggaa ggaaaaaata tggagaactc acatccactc ttcattgtcc 8940tcaaacagaa gtaacccatt ttgttctgct cacagcccat tggccagaac taactgtatg 9000gccccaatct aattgcaaag gagtctggga agtacagcac agcacatgga tctttggaaa 9060gcgttaattt tctctgccaa aggcttcttt tgttgttgtt gttgtttgaa atggagtccc 9120gctccgtcac ccgggctgga gtgcagtggc cctatctcag ctcactgcaa cctccacctc 9180ccaggttcaa gcactcctcc agccttggcc tcccaagtag ctaggattac aggcgtgtgc 9240catcatacct ggctattttt tttgtatttt tagtagagac agggttttac cgtgttggcc 9300aggctggtct caaactcttg gcttcaagtg atccgcctgc ttcggtctcc caaagtgttg 9360ggattacagg cgtgagccac tgtacccggc caatactggt gttttctgac ctcagcgttt 9420ttcttttctc ctgccatgta ctctccctag agatttcatc tactcccaag tcttccactg 9480ctcctatctg ctgatgactc ccaaaactca gtctccagcc gagacttctc tcctgggctt 9540gagacatatg tatccaactg ccagaacatc tccagtggac agcctttggg cacaaggcca 9600cactagcttg tgggtacaag taatcaccca aagtcaattt cagtggctct ccactcccac 9660attttttcaa cccctggaaa tgttcccttc ccaaatactc tgagtctctc ttctcttttg 9720atgactgtgg ttttgtgact ggatggtagc tcctgttgct ttttttcctt tcaaatgaat 9780tttctcttag gaggcttctt agccattaag caaatagacc tcaactgggc ttgccctatg 9840cctatctgaa acccagctta ggttcgagtt aggactcctg ttaatctgag ctctcacttc 9900ctgtcccaac ctgcttattt tttttggtga aagaaaatat catcccccta gttgctcaga 9960aacctgggaa tcattgggat tttttcctct ccttcacctt cctcatccaa tcagtcacca 10020agtgctatca actctgctgc cttagtagcc ctcaatatat acttacctat caaccatcac 10080tgctatgcca tacttcaggc cttcatttct cacttggatt attaaaatat ccctaaatag 10140ttcctctgcc ttctctctgg ccaccctcct gagtgatctc tccatcatgt acctttcagt 10200gactgcttgc acaagcccct ttgtgacttg gtcatagtct gctctcttga accaccagag 10260ccaagcacct gggttctgat tctggttgca actcttactg cgtgatgatg gacaggccac 10320ttgatctcct caaacctcag ttcctaaatc aaatgaatga ttgaattcag tcacttaact 10380ttgtatgtag taggtaggca ctgtgcaaaa catactagtg gatatagaga tgaataagaa 10440aaagcccctg cactcaaaga gctctcggat tcatcaacaa attattgtgc agttagatag 10500taagtgctat aatccaggaa tatacagtgt tgtgtgaata atgtggaatc agtttatctc 10560cagagcagaa aaaggtgaag gccgaagaag gcattcagag tgatactgga gctgtgtagc 10620aggggctact actgttccct caaatccttt cttctcttct tcctgagtaa tagagtcctt 10680tttcagctag gcatacagcc atccaaaata aaaactatat ttcccagcct ccttttcagc 10740taagtgtagc catgtgacta agttgtggcc aatgggatgt cagtacaagt ggtaatggca 10800atatctggga tgtgtcttta aaaggaagga acatgtcctt ctccttttcc tccttcctgt 10860tccctgggag gtgaacttgg tagctggagg tgaagcagct ggaacttgga tatgagcgtc 10920ttgttgatga taatagtgca acaagataaa agcagcccga gctggcctac atttacatga 10980gaaggaaata agactccatt ttgtttaagc tattctcttg catatatata tacacatata 11040tatcagctaa gtgtagccat gtgactaact tgtggccaat gggatgtcag tatgagtggt 11100aagagcagta tcttgtatat atatatgtgt atatatacac acacacatat atatacatat 11160atgtatgcaa gaatataaaa tatacatgtg tgtgtgtata tatatacaca catatatatg 11220tcccttgcag ccaagtctaa ttctaactaa tacaaactaa ctctaaaaaa tgaatatata 11280ttcaccaggg gataggctat ttcaagcaga gggaagcctg tataaaggct cagggaatgc 11340tgtggtttta tgtggcagca gatgagactg gaaatgagtc aggatgagcc acagtggagg 11400atgaattaaa tgggcaggag tgtggtagaa agacctgttg gaggctatga atgcaatcaa 11460ggtgacagac aactggtgca atgatggtag tggaaatgga ggagagggga ttgattcaag 11520atgcatttag gaccaagaat cgggagcttg tgaacgtgtg tatgagtact gtagacggag 11580tgggtgtgtc atcagagaag atctgagcat ttgggcttgc tctcctcaga ggccctgcga 11640gtggagttca gcttttcctc atggggcaaa tcttactttc gctccagttc ctggggctca 11700gagtccctgg cccagatgcc tcttgccatc tcatcttcac cctgcctggc ttcccttgct 11760tgttccagga ttgtttcata aagagggatg tggttggtct ttaaccctat gaatgctggc 11820tgaggatgcc tgcggaacct gtagtgaagc tttcaggggc tgctcgggtt ctggctggta 11880ggtgaacact gtccatcttg ccggctggga cacagtgact ctgggtagtt gtgtaagaga 11940ggggcccttg gcagacaaac aggttcttct ctgttggtgg gccagccagc aggtcagtgg 12000gaaggttaaa ggtcatgggg tttgggagaa actgggtgag gagttcagcc ccatcccccg 12060taaagctcct gggaagcact tctctactgg ggcagcccct gataccaggg cactcattaa 12120ccctctgggt gccagggaaa gggcaggagg tgagtgctgg gaggcagctg aggtcaactt 12180cttttgaact tccacgtggt atttactcag agcaattggt gccagaggct cagggccctg 12240gagtataaag cagaatgtct gctctctgtg cccagacgtg agcaggtgag cagctggggc 12300agagggatgg gggtcacagt cctaagggag ggcattgcag gtggcctcag gggagagcct 12360ggggtggccc ctaagacgtc ctcttggaac attttggcag agttgcctct tcgccctcat 12420tatggctcag tttttccacc atgaaatggg agggagggag acaggtgggc aggggagagg 12480tggtagaagt ggcctagaga actgttcctg gggtctggga cctttgcgaa ggggttagag 12540caccacgctc cctgctatgt gactgaggta gcaagagcac gccctcttcc catgtttgag 12600gaagacaccc tagcctcctt gactcaccta ggtcagtcct cttgagcccc aacagctctg 12660tgctccccag cccaaggaag gggtaacagg atttcgggca gttgcccctg cagaggcccc 12720ctgggcaagt cccctgcgcc atgtcccttc gtctccttct tcccctaacc aggcctccct 12780ccacctgtct tctcagagca gataatggca agcatggctg ccgtgctcac ctgggctctg 12840gctcttcttt caggtgggtc tccgaccctg acttcaacgt gggggtgtgg gtggaggctg 12900gccagagggc cctgtccacc ctgggggagg agagcccagg ccctgattac ctagtccctc 12960tccacagcgt tttcggccac ccaggcacgg aaaggcttct gggactactt cagccagacc 13020agcggggaca aaggcagggt ggagcagatc catcagcaga agatggctcg cgagcccgcg 13080tgagtgccca ggggaagggg tgtaggcgaa gggaggagac agctgggcca tgccatgatg 13140acctgcctct gctgcctcaa cctctgtggc cgctgctggg acagaggaaa ggagcggtgc 13200tagctctgtc tgcagatccc ggccatcctg ggctctttag cgccctctgc ctgcagcccc 13260cgccttgaca actccgtagc tgttgccccc ttgctcactg aggcgcggga cctgggatca 13320atcgggagga cgcccgctgc agtccccaga atcaaaggat gatgtggcgc atctatgttt 13380ctttggagag tgttgtaggt ctggatttgt atgggcaatg tgtttgtgct tcgtgcgtga 13440gttgttactg gccagggcta ggacaagagc cctcgaccct ggggccaacg ccctgcgtcc 13500ttggttcccc cagaggatca gtgcgcgatg acttggggac aaaggagatg atgggggcta 13560gcagtctgac ggcctggata tctgtcccct tctccaggac cctgaaagac agccttgagc 13620aagacctcaa caatatgaac aagttcctgg aaaagctgag gcctctgagt gggagcgagg 13680ctcctcggct cccacaggac ccggtgggca tgcggcggca gctgcaggag gagttggagg 13740aggtgaaggc tcgcctccag ccctacatgg cagaggcgca cgagctggtg ggctggaatt 13800tggagggctt gcggcagcaa ctgaagccct acacgatgga tctgatggag caggtggccc 13860tgcgcgtgca ggagctgcag gagcagttgc gcgtggtggg ggaagacacc aaggcccagt 13920tgctgggggg cgtggacgag gcttgggctt tgctgcaggg actgcagagc cgcgtggtgc 13980accacaccgg ccgcttcaaa gagctcttcc acccatacgc cgagagcctg gtgagcggca 14040tcgggcgcca cgtgcaggag ctgcaccgca gtgtggctcc gcacgccccc gccagccccg 14100cgcgcctcag tcgctgcgtg caggtgctct cccggaagct cacgctcaag gccaaggccc 14160tgcacgcacg catccagcag aacctggacc agctgcgcga agagctcagc agagcctttg 14220caggcactgg gactgaggaa ggggccggcc cggaccccca gatgctctcc gaggaggtgc 14280gccagcgact tcaggctttc cgccaggaca cctacctgca gatagctgcc ttcactcgcg 14340ccatcgacca ggagactgag gaggtccagc agcagctggc gccacctcca ccaggccaca 14400gtgccttcgc cccagagttt caacaaacag acagtggcaa ggttctgagc aagctgcagg 14460cccgtctgga tgacctgtgg gaagacatca ctcacagcct tcatgaccag ggccacagcc 14520atctggggga cccctgagga tctacctgcc caggcccatt cccagctcct tgtctgggga 14580gccttggctc tgagcctcta gcatggttca gtccttgaaa gtggcctgtt gggtggaggg 14640tggaaggtcc tgtgcaggac agggaggcca ccaaaggggc tgctgtctcc tgcatatcca 14700gcctcctgcg actccccaat ctggatgcat tacattcacc aggctttgca aacccagcct 14760cccagtgctc atttgggaat gctcatgagt tactccattc aagggtgagg gagtagggag 14820ggagaggcac catgcatgtg ggtgattatc tgcaagcctg tttgccgtga tgctggaagc 14880ctgtgccact acatcctgga gtttggctct agtcacttct ggctgcctgg tggccactgc 14940tacagctggt ccacagagag gagcacttgt ctccccaggg ctgccatggc agctatcagg 15000ggaatagaag ggagaaagag aatatcatgg ggagaacatg tgatggtgtg tgaatatccc 15060tgctggctct gatgctggtg ggtacgaaag gtgtgggctg tgataggaga gggcagagcc 15120catgtttcct gacatagctc tacacctaaa taagggactg aaccctccca actgtgggag 15180ctccttaaac cctctgggga gcatactgtg tgctctcccc atctccagcc cctccctctg 15240ggttcccaag ttgaagccta gacttctggc tcaaatgaaa tagatgttta tgatagaagt 15300ttgcctggcg tgactctcat ttggaccatg tctgaaagca gtggcctcac cactatcccc 15360aaagcacacc catcacccac tccattccct tgctgctctt tctcatccac ccactcccag 15420tccaggtctg tcaaaggggg tctggctggg ctctgcttca gggatcctgg ctagacaacg 15480gctgtctgtc acacctggca ggagggcctg ggttacgggc ccttcctctg cacctgcact 15540gttcactagc ctgctccccc acaggacact gtgcatggaa tgcaggctgt gtctggaaga 15600gctgtggccc tggtggacct aagattcctg aggtgggctg cctcctttgt tcctgctgtt 15660ctagagtttg aatggcctct ttttatgccg gactctcttc tggggactcc cctcactcag 15720gggcaccaat gctccctata gatcccctgg gaactgaaac tggggtgtgg tggaggacgt 15780ggaaagggta aacacagctc cttgtctttg gacttccctg tccggccccc tttcctccca 15840gctcagccta ctgtccccgg gttctcagca cctgcctgct ccccaacccc atagcacaga 15900ccccacacat atgtaggctc atcatgcctg caggctggtc ttccctgaca ccgtggattt 15960tgacaatgtt ggcaacagaa ctgggttgtg gacccagcac 16000526656DNAHomo sapiens 5tcgagctgtt ttacctaagc tttccactca tgattaacta atgaagatta caatgattac 60atggtagaac ccatacacaa accactagac ctggcaccag ggggttttgc aagaaagagg 120gtaagattct agctacatgc gaacagccca aatactctga tcttaaactt acctcctttc 180tggctaaagg caggaatttc aaaatctagc tcaggaatcc ttgtggcagc agagtcagtc 240ttcaccactt ctctgttcat gtcctgggaa gaaaagaata cgttcagtac aaagagactg 300cagaacagct tctcctcaag taataaccag accgtcccct tccatcagac cgcagcccag 360ctgtctctcc ctctcgccac tccacagggt ccagactgct cctttcgaat agtacaggtg 420gtgtgagcca ggctgtccaa atggtgagaa tactattcat aacaactact gctcatttca 480taccctcaga caacatttac aaagcatttt cacaaatctt atctcacttg agctgatgac 540aacaccagta ccattttaca aaagaaacta aggctaggag acataggggg atttatttaa 600gatccgaccg cctggaccag agctctgacc ccagtaccga atctctctct agaatactga 660ggtacctgct ctgaggtcct ctcacctcca gagccctgac agacaaagtg tagcgcactc 720cctggtcctg gatcctgcct gccgactgga tctccgtgtt gttccagcca cagtgctcgc 780aggaaaagga gctcactatt atttctctga agaagggaat cttggtgagc aggaggcgcg 840tcatgccctg gtgaagtaga aagtagctaa ggaaaaaaat caatgaaact ccctttcccc 900gccctactat ctccgggcgc tcctcggggt ccccggccgg gcccacctgg ctgggtctga 960cccccggctc gtcccggcac aagccctacc cacccgccgc tcgcggccgc gcccccgcct 1020cacattgcag taacagttca tgcatagcga ctcgatctcg gtgggctgct gctcctcgtc 1080ctcggcgctg atgggccgga acaggtgatc aggggcaggc ggcggggccg gggcgggcga 1140cggggcgacg gcagcccccg ggggccctgg ttccacagcc ccgctggccg ccatggccac 1200cacgcgcaat tcagacctcg gcttcctact tccgccgctc tcgcggcccc acccctctcc 1260cgtcacttcc gccagctgcc accaccccaa aaaaagccgc agactccggg cactcgggga 1320acaatgtcgt caagggctcc cggttcccgg gtttatatgg ctcgaacctt acatcgccag 1380ccggatccac ctcaactcag ccgtcacttc ccctaagacg tcctcttaga accagggcac 1440ttttttgctc cttcacaata gctccaccgc acggggttct attggtccct acgtcggtct 1500acaagagcct tgacgacaag gggctggact tgtccctgag gaatccctga gaaggtgcgc 1560ccggagccgg gctgcttggt tccagtgttg ggccacatac tgcttgcgtg ctaggtcgcc 1620cctccgggtg gctcagcctc ttcccctctc tcacaatccc tgaatccctc tgtccctttc 1680tgttcttccc actccctatt ctgtcctcat cactaccttc cccaatccca gcctcaagta 1740cacagccctc attttcactc ctacctcctc atccccatca tagcccttga cctatgcttt 1800tccagtttcc taacaacaac aaaaacacct tttattattt ctacctttct agcacttcct 1860ctctccaggt gctgggtcca caacccagtt ctgttgccaa cattgtcaaa atccacggtg 1920tcagggaaga ccagcctgca ggcatgatga gcctacatat gtgtggggtc tgtgctatgg 1980ggttggggag caggcaggtg ctgagaaccc ggggacagta ggctgagctg ggaggaaagg 2040gggccggaca gggaagtcca aagacaagga gctgtgttta ccctttccac gtcctccacc 2100acaccccagt ttcagttccc aggggatcta tagggagcat tggtgcccct gagtgagggg 2160agtccccaga agagagtccg gcataaaaag aggccattca aactctagaa cagcaggaac 2220aaaggaggca gcccacctca ggaatcttag gtccaccagg gccacagctc ttccagacac 2280agcctgcatt ccatgcacag tgtcctgtgg gggagcaggc tagtgaacag tgcaggtgca 2340gaggaagggc ccgtaaccca ggccctcctg ccaggtgtga cagacagccg ttgtctagcc 2400aggatccctg aagcagagcc cagccagacc ccctttgaca gacctggact gggagtgggt 2460ggatgagaaa gagcagcaag ggaatggagt gggtgatggg tgtgctttgg ggatagtggt 2520gaggccactg ctttcagaca tggtccaaat gagagtcacg ccaggcaaac ttctatcata 2580aacatctatt tcatttgagc cagaagtcta ggcttcaact tgggaaccca gagggagggg 2640ctggagatgg ggagagcaca cagtatgctc cccagagggt ttaaggagct cccacagttg 2700ggagggttca gtcccttatt taggtgtaga gctatgtcag gaaacatggg ctctgccctc 2760tcctatcaca gcccacacct ttcgtaccca ccagcatcag agccagcagg gatattcaca 2820caccatcaca tgttctcccc atgatattct ctttctccct tctattcccc tgatagctgc 2880catggcagcc ctggggagac aagtgctcct ctctgtggac cagctgtagc agtggccacc 2940aggcagccag aagtgactag agccaaactc caggatgtag tggcacaggc ttccagcatc 3000acggcaaaca ggcttgcaga taatcaccca catgcatggt gcctctccct ccctactccc 3060tcacccttga atggagtaac tcatgagcat tcccaaatga gcactgggag gctgggtttg 3120caaagcctgg tgaatgtaat gcatccagat tggggagtcg caggaggctg gatatgcagg 3180agacagcagc ccctttggtg gcctccctgt cctgcacagg accttccacc ctccacccaa 3240caggccactt tcaaggactg aaccatgcta gaggctcaga gccaaggctc cccagacaag 3300gagctgggaa tgggcctggg caggtagatc ctcaggggtc ccccagatgg ctgtggccct 3360ggtcatgaag gctgtgagtg atgtcttccc acaggtcatc cagacgggcc tgcagcttgc 3420tcagaacctt gccactgtct gtttgttgaa actctggggc gaaggcactg tggcctggtg 3480gaggtggcgc cagctgctgc tggacctcct cagtctcctg gtcgatggcg cgagtgaagg 3540cagctatctg caggtaggtg tcctggcgga aagcctgaag tcgctggcgc acctcctcgg 3600agagcatctg ggggtccggg ccggcccctt cctcagtccc agtgcctgca aaggctctgc 3660tgagctcttc gcgcagctgg tccaggttct gctggatgcg tgcgtgcagg gccttggcct 3720tgagcgtgag cttccgggag agcacctgca cgcagcgact gaggcgcgcg gggctggcgg 3780gggcgtgcgg agccacactg cggtgcagct cctgcacgtg gcgcccgatg ccgctcacca 3840ggctctcggc gtatgggtgg aagagctctt tgaagcggcc ggtgtggtgc accacgcggc 3900tctgcagtcc ctgcagcaaa gcccaagcct cgtccacgcc ccccagcaac tgggccttgg 3960tgtcttcccc caccacgcgc aactgctcct gcagctcctg cacgcgcagg gccacctgct 4020ccatcagatc catcgtgtag ggcttcagtt gctgccgcaa gccctccaaa ttccagccca 4080ccagctcgtg cgcctctgcc atgtagggct ggaggcgagc cttcacctcc tccaactcct 4140cctgcagctg ccgccgcatg cccaccgggt cctgtgggag ccgaggagcc tcgctcccac 4200tcagaggcct cagcttttcc aggaacttgt tcatattgtt gaggtcttgc tcaaggctgt 4260ctttcagggt cctggagaag gggacagata tccaggccgt cagactgcta gcccccatca 4320tctcctttgt ccccaagtca tcgcgcactg atcctctggg ggaaccaagg acgcagggcg 4380ttggccccag ggtcgagggc tcttgtccta gccctggcca gtaacaactc acgcacgaag 4440cacaaacaca ttgcccatac aaatccagac ctacaacact ctccaaagaa acatagatgc 4500gccacatcat cctttgattc tggggactgc agcgggcgtc ctcccgattg atcccaggtc 4560ccgcgcctca gtgagcaagg gggcaacagc tacggagttg tcaaggcggg ggctgcaggc 4620agagggcgct aaagagccca ggatggccgg gatctgcaga cagagctagc accgctcctt 4680tcctctgtcc cagcagcggc cacagaggtt gaggcagcag aggcaggtca tcatggcatg 4740gcccagctgt ctcctccctt cgcctacacc ccttcccctg ggcactcacg cgggctcgcg 4800agccatcttc tgctgatgga tctgctccac cctgcctttg tccccgctgg tctggctgaa 4860gtagtcccag aagcctttcc gtgcctgggt ggccgaaaac gctgtggaga gggactaggt 4920aatcagggcc tgggctctcc tcccccaggg tggacagggc cctctggcca gcctccaccc 4980acacccccac gttgaagtca gggtcggaga cccacctgaa agaagagcca gagcccaggt 5040gagcacggca gccatgcttg ccattacctg ctctgagaag acaggtggag ggaggcctgg 5100ttaggggaag aaggagacga agggacatgg cgcaggggac ttgcccaggg ggcctctgca 5160ggggcaactg cccgaaatcc tgttacccct tccttgggct ggggagcaca gagctgttgg 5220ggctcaagag gactgaccta ggtgagtcaa ggaggctagg gtgtcttcct caaacatggg 5280aagagggcgt gctcttgcta cctcagtcac atagcaggga gcgtggtgct ctaacccctt 5340cgcaaaggtc ccagacccca ggaacagttc tctaggccac ttctaccacc tctcccctgc 5400ccacctgtct ccctccctcc catttcatgg tggaaaaact gagccataat gagggcgaag 5460aggcaactct gccaaaatgt tccaagagga cgtcttaggg gccaccccag gctctcccct 5520gaggccacct gcaatgccct cccttaggac tgtgaccccc atccctctgc cccagctgct 5580cacctgctca cgtctgggca cagagagcag acattctgct ttatactcca gggccctgag 5640cctctggcac caattgctct gagtaaatac cacgtggaag ttcaaaagaa gttgacctca 5700gctgcctccc agcactcacc tcctgccctt tccctggcac ccagagggtt aatgagtgcc 5760ctggtatcag gggctgcccc agtagagaag tgcttcccag gagctttacg ggggatgggg 5820ctgaactcct cacccagttt ctcccaaacc ccatgacctt taaccttccc actgacctgc 5880tggctggccc accaacagag aagaacctgt ttgtctgcca agggcccctc tcttacacaa 5940ctacccagag tcactgtgtc ccagccggca agatggacag tgttcaccta ccagccagaa 6000cccgagcagc ccctgaaagc ttcactacag gttccgcagg catcctcagc cagcattcat 6060agggttaaag accaaccaca tccctcttta tgaaacaatc ctggaacaag caagggaagc 6120caggcagggt gaagatgaga tggcaagagg catctgggcc agggactctg agccccagga 6180actggagcga aagtgagatt tgccccatga ggaaaagctg aactccactc gcagggcctc 6240tgaggagagc aagcccaaat gctcagatct tctctgatga cacacccact ccgtctacag 6300tactcataca cacgttcaca agctcccgat tcttggtcct aaatgcatct tgaatcaatc 6360ccctctcctc catttccact accatcattg caccagttgt ctgtcacctt gattgcattc 6420atagcctcca acaggtcttt ctaccacact cctgcccatt taattcatcc tccactgtgg 6480ctcatcctga ctcatttcca gtctcatctg ctgccacata aaaccacagc attccctgag 6540cctttataca ggcttccctc tgcttgaaat agcctatccc ctggtgaata tatattcatt 6600ttttagagtt agtttgtatt agttagaatt agacttggct gcaagggaca tatatatgtg 6660tgtatatata tacacacaca catgtatatt ttatattctt gcatacatat atgtatatat 6720atgtgtgtgt gtatatatac acatatatat atacaagata ctgctcttac cactcatact 6780gacatcccat tggccacaag ttagtcacat ggctacactt agctgatata tatgtgtata 6840tatatatgca agagaatagc ttaaacaaaa tggagtctta tttccttctc atgtaaatgt 6900aggccagctc gggctgcttt tatcttgttg cactattatc atcaacaaga cgctcatatc 6960caagttccag ctgcttcacc tccagctacc aagttcacct cccagggaac aggaaggagg 7020aaaaggagaa ggacatgttc cttcctttta aagacacatc ccagatattg ccattaccac 7080ttgtactgac atcccattgg ccacaactta gtcacatggc tacacttagc tgaaaaggag 7140gctgggaaat atagttttta ttttggatgg ctgtatgcct

agctgaaaaa ggactctatt 7200actcaggaag aagagaagaa aggatttgag ggaacagtag tagcccctgc tacacagctc 7260cagtatcact ctgaatgcct tcttcggcct tcaccttttt ctgctctgga gataaactga 7320ttccacatta ttcacacaac actgtatatt cctggattat agcacttact atctaactgc 7380acaataattt gttgatgaat ccgagagctc tttgagtgca ggggcttttt cttattcatc 7440tctatatcca ctagtatgtt ttgcacagtg cctacctact acatacaaag ttaagtgact 7500gaattcaatc attcatttga tttaggaact gaggtttgag gagatcaagt ggcctgtcca 7560tcatcacgca gtaagagttg caaccagaat cagaacccag gtgcttggct ctggtggttc 7620aagagagcag actatgacca agtcacaaag gggcttgtgc aagcagtcac tgaaaggtac 7680atgatggaga gatcactcag gagggtggcc agagagaagg cagaggaact atttagggat 7740attttaataa tccaagtgag aaatgaaggc ctgaagtatg gcatagcagt gatggttgat 7800aggtaagtat atattgaggg ctactaaggc agcagagttg atagcacttg gtgactgatt 7860ggatgaggaa ggtgaaggag aggaaaaaat cccaatgatt cccaggtttc tgagcaacta 7920gggggatgat attttctttc accaaaaaaa ataagcaggt tgggacagga agtgagagct 7980cagattaaca ggagtcctaa ctcgaaccta agctgggttt cagataggca tagggcaagc 8040ccagttgagg tctatttgct taatggctaa gaagcctcct aagagaaaat tcatttgaaa 8100ggaaaaaaag caacaggagc taccatccag tcacaaaacc acagtcatca aaagagaaga 8160gagactcaga gtatttggga agggaacatt tccaggggtt gaaaaaatgt gggagtggag 8220agccactgaa attgactttg ggtgattact tgtacccaca agctagtgtg gccttgtgcc 8280caaaggctgt ccactggaga tgttctggca gttggataca tatgtctcaa gcccaggaga 8340gaagtctcgg ctggagactg agttttggga gtcatcagca gataggagca gtggaagact 8400tgggagtaga tgaaatctct agggagagta catggcagga gaaaagaaaa acgctgaggt 8460cagaaaacac cagtattggc cgggtacagt ggctcacgcc tgtaatccca acactttggg 8520agaccgaagc aggcggatca cttgaagcca agagtttgag accagcctgg ccaacacggt 8580aaaaccctgt ctctactaaa aatacaaaaa aaatagccag gtatgatggc acacgcctgt 8640aatcctagct acttgggagg ccaaggctgg aggagtgctt gaacctggga ggtggaggtt 8700gcagtgagct gagatagggc cactgcactc cagcccgggt gacggagcgg gactccattt 8760caaacaacaa caacaacaaa agaagccttt ggcagagaaa attaacgctt tccaaagatc 8820catgtgctgt gctgtacttc ccagactcct ttgcaattag attggggcca tacagttagt 8880tctggccaat gggctgtgag cagaacaaaa tgggttactt ctgtttgagg acaatgaaga 8940gtggatgtga gttctccata ttttttcctt ccttccccta cagtggaagc aaagaactcc 9000aagctggcac tattacaaga gggagagggt ctatattctg agttagtgct tggagcagca 9060cctcccacct aaaagagggg cagagaagta gaagtataat caaaataaag aagaatcaca 9120gaaagaaaat atattagtta aattatatat ttggctgctc tgagagagac ccaaaataac 9180agtggcttac aaaagctgga aatgtatgtt ttctcccata caaaacttca cgctggtatg 9240ggagcactgc tccacaaagc tgtccagggt cctaagttcc ttctgtctgg aggttctaat 9300atccctatgt gtttcccttg tccacatgat ctaagatagc ttaccaccat gtccacatcc 9360agccactgga aagggggcag ggtggaggag aggagagtgt agaatgtact ctcctttctt 9420tctttttttt tttgagatgg agtttcgctc ttcttgccca ggctagagtg cgatggcaca 9480atctcggctc atcacaacct ccacctccca ggttcaagcg attctcctgc ctcagcctcc 9540tgagtagctg ggattacagg catgcgccac gacgcccagc taattttgta tttttagtag 9600agacgaggtt tctccatgtt ggtcaggcta gtcttgaact cccggcctca ggtgatccgc 9660ccacctcggc ctcccaaagt gctgggatta caggcgtgag ccactgcacc cagccactct 9720cctttctttt aaggacaaaa cctaacttct tctcccatct cactggtcag aactacttgt 9780aaggaaggtt aggaaatgtc atctttgttt tgaatgggcc tgagttcaat gatcagttaa 9840aaatcaggga ttctaccctc atacattgct ggtgggattg tacaatggtg caatttcttt 9900ggaaaacagt tcggcagttc attaatggtt aaacatggag gagttctcct atgactcagc 9960aattctattc ctaggtataa aaccaagaca catgaaaata cacatctgca caaaaacttg 10020tgcattaatg ttcatggcaa cattattcat aacagtcaag aaaatggaaa caacccaaat 10080gtccatcaat tgatgaatgg ataaacaaaa tgttacctat ccatatagtg gaatattatt 10140tggcaataaa aagggttgaa gtactgatac ctgctacacc acagatgaac ctggaaaaca 10200ttatgctagg ggaaaaaagc cagtcacaaa agactacatg ttgtaaaatc tcatttgtat 10260aaaatgtcca gaaaagacaa ataggtagag acagaaagta gactggtggc tgcctagggc 10320tattgagggg aggggaggtt gattggggag aaatagtgaa taactgctaa tgggtgtggg 10380gtttctttgt gggatgatga aaaaagttct aaacttagtt tgtggtgata gttgtacaaa 10440tctgtgaata tactaaaaac cattcaattg tagtgaatgt tatggtatgt gaattatatc 10500tcaataaaga tatttctaaa atcagggatt ttataactga gaaagaaggg aagaataaag 10560ttgcagagga gacttgcagc ctctgccaca ccaaggacga aaaggtttca agagtgaaga 10620attaatggct tcccaccatg gcagactgag ctgagctgag gctgtgtttt caatgctttt 10680ctatctctac tctttttttt tttttttttt ttttgagaca gagccttgct ctgtcaccag 10740gccatacagt ggtgtgatct cggctcactg caactaccgc ctcccgggtt caagcgattc 10800tcttccctca gcctcccaag tagctgggag tgcacaccac cacacccagc gaatttttgt 10860atttttaata gagatggagt ttcaccatgt tggccaggct gatctcaaac tcctgacccc 10920aagtgatccg cccacctagg cctcccaaag tgctgggatt acaggtgtga gccatccccc 10980tggcctctat ctctactcct aaaagaacca tttctgacca cttaacaccc attagaatgt 11040tattttaaaa aataaaataa aagccaaaaa tagcaagtgt gggtgaagat gtggagaagc 11100tggaccgctt gtacgctgct gctggaaagg taaaatggtg cagctatggt ggagaacaat 11160acggcagtcc ctcaaaaaat ttaatctaga attaccttat gacccagcaa ttccacctct 11220gggcatatat ccaaaagata aaagcaggga ctggagcaga tatttgtatg ctcgtgttcc 11280tagtagtatt atccacaata accagggggt ggaaacaacc caaatgtcca ttgacagatg 11340aatgaataaa caaattgtgg tatatacaga caacaaaata tcacttagcc ttaaataatg 11400acaatcgggg gccaggcata gtggctcacg cctgtaatcc cagcactttg ggaggccgag 11460acgggcagat cacttgaggc cggaagtgca agaccagcct ggccaatatg gtaaaacccc 11520gtctctacta aaaatacaaa aattagccag gtgtggtggt gcatgcctgt cattccagct 11580acttgggagg ctgaggcacg agaattgctt gaacctggaa ggcagaggct gcagtgaact 11640gagattgtgc tactgccctc cagcctgggt gacagagcga gactgtgtct caaaaaaaaa 11700aaaaaagaaa agaaaagaaa aggaaatctg acacatgcta taacgtggat gacccttgaa 11760gacatgatgc taagtgaaac aagccagtcg tagaacgaca catactgtga ttctgaggta 11820tccagaatag tcagatttgt agagacagaa agtataattc tggtttctag gagaagtgag 11880gaaggagaag gtattggtta ataggtacaa gtttctgttt gggaagatta aaagtttctg 11940gagctagata gtggtgcaca acagtgtaaa tgtatttagt gctactgggc tatatactta 12000gctacaatgt acaatagcta caatggtaat ttttatgtta tgtatatttt gccacaattt 12060aaaaaggaat ttctcatact ctaatggttt tttgctgata acccaaactt aaagagcctg 12120tgctgtaaaa tacaactgcg ctggattgtc tttttgcaaa tagcattatc agtgcccttt 12180ctaaggtctt ttatgagatg cagaggggac acttggaact attaatacat tttctagaat 12240cccttctcta tatgagccta ggttagagtc agccagcgtg agacggaaga ggagaggcca 12300ttatgtttca atgccggttg cagacagaag catggacagg cttggagttt aaagcagctt 12360ctgggtgacc ttcccaggaa tcacatgcat tgatattgca ggcataataa ggcgagcttc 12420ccatttgtag ccacttacta atgagcgttt gagagtcact ccttctgaac tgcaaaccaa 12480ggtggtcact cctccagccc ttccaagagt tggataaacc tttaattttt tgttttaaag 12540cctttcatgc ttgaaatacg tggaatggct tttgtttttc tgactgaacc tggattgata 12600caatgggtag atgcaacaca cactcatgct tccctgtttg gtggcatctt atagtttcag 12660tgttgaacat gttttgagtt ggttggaggt atatgtatat gtgtatgtgg tatgtgcttt 12720gtgtgtggaa ccatttgaaa taattatagc caacatgaca cttcactctg aatacttcaa 12780catacattgt gaaaaataag gactctttta caaaagaaaa aaacattatc atacctttga 12840aaattaacac aattccttaa tatcatctaa catatcctac atattcagat tttccccaaa 12900atgctttcat agttgttttt ctctctctgt aaattcaagc tctaacacat ctcatctggt 12960tgttatccca ctttagtttt taaatttaga accgttctcc ccaaccccac catttgtttg 13020ttttcatgac attgattttt ttttggaaga gtgcaaggag gacatcttgt aaaatgtccc 13080acattctgga tttgagtgat tactttctca tgattagatt aaggttaaac atttttggca 13140aaaatactag gtgatatctg gcccttctta ctgtatccta gctggaggtt gcactctatt 13200tagtgatgtt aagtttgatc actccattaa agtgataaca accagatttc tccatggcta 13260gtaaagtttg gttttttacg ttattgataa gtaatttgtg gagcaatact ttgaagccat 13320gtgagcatcc cattctgcaa caatttttca cccaatggtt ttagcattca ttgatgatcc 13380ttgcctgaat cgaatattat cctgagagtt gccagatagt gatttttaaa aaattctagg 13440ccaggtgcag tagctcatgc ctgtaatccc agctctttgg aaggctgaga tgggaggatc 13500acttgaaccc aggaatttga gaccagcctt ggcaacatgg caaaacctgt ctctacaaaa 13560aaaaaaaaaa aaaaaaaaaa actaaaaata aaatacaaaa attagcctag catggtggca 13620cacatgcttg tagtcccagc cactcaggag gctgaggtgg gaggatcacc agagcctggg 13680aggttgaggc tgcagtgagc cgtgatcaag ccactgcact ccagcctggg ccacagagtt 13740gcccaaaaca aaatgaaaac ttaaagaatt ccatcattcc agctacattt attatccgat 13800ttcttctgta agtaagagct tttagggaac ttgttcttaa atccagagac tttgaacaga 13860ttccttccag gtgaacttgt ttgtgctgcc ctttccttga ctgtcgtttt gtatcacagt 13920gtttgcggat agctgcattt ttaagagagt tgcccctcta ctttgttttg tgtttttgtt 13980ttttaataca caagaattgc tgactcattc tggatgtgta gtaagaagga ttttaaaatg 14040tagattggta aaggttagca ggtaccttat tccttactgc tcagcagcag cacttttgcc 14100ttaaacacat ggattaagca agtgtatggg tctcaaatag acacttgata tgcaccaagt 14160gagaagatct tctagagtct ttcttttgtg acctgttctt taaagcaaca ttatgacttc 14220tccttctgaa cagtaagtat tttttttttt tttttttttt ttttggcaga gtctcactct 14280gtcacccagg gtggctggag tacagtggcg tgatctcggc tctcggcaac ccagtcactt 14340gggttcaagc gattctcctg cctcagcctc ctgagtagct gggattacag gcgtgtgcca 14400ccgtgcctgg ctaatttttg tatttttagt agagatgggg gttattccat gttggccagg 14460ctggtcttga actcctgacc tcaggtgatc cacccacctc ggactcccaa agctcccaaa 14520gtactgggat tacaggtgtg agccaccaca cccgggccaa gtaaatcttg ttacaaattg 14580ttctccttca gtcttgtctt ctaagaactc agatgtaaac tgtgaggtag cagtctttac 14640ttggtgttcc tggactccat ctcagaacgc accaaaaaca tctatatgat tgtggagcca 14700ctctacattg tttctactgc tatcaccaga ccttaatgga ggtgtgggtt ttttaaaaat 14760caatatacat ctcaaataca tttcagaaag aaaggtgttt atgcttaacg cagtgttaaa 14820actatcccag caagaaagta gtaacctgtg taagcatttt tttgtttctt tgcttgtttg 14880tttatttgtt tttgagacag gatctcactt tgtcacccag gctagagtgc agtggcatga 14940tcacagttca ctgcagcctc aacctcccag gctcaagcaa tcctccagcc tctgcctcaa 15000gcaatcctcc agcctctgcc tcccaagaat ctgtagtttc tgggactaca ggagtgtccc 15060acaacaccca gctgattttt tatttttttg gtagagaaag gatctcacta tgttgcccag 15120gctggccttg aactcctggg ctcaagcaat cctcctgcct cagcctccca aagcactggg 15180attacaggca tgaaccacca tgcctggcct tttgtttgtt ttgaaataag ttctatttgt 15240caaaaagaaa gagaattaaa tagaacatct ataagtgaaa aaaatatagt cattataata 15300taaaacacat ttagctacag ttggagagag aattagtaag ctagaaggta gatctgagaa 15360aatcacacag aaagaatcac agaaataaaa aggaggatat tgaccaggca cggtggctca 15420tgcctgtaat cccagcactt tgggaggccg aggcgggtga tattggccag gcatggtggc 15480tcatgcctat aatcccagca ctttgggagg ccgaggtggg tggatcacct gaggtcagga 15540gtttcagacc agcctggcca atatggtgaa accccgtctc tactaaaaat acaaaaatta 15600gccgggcgtg gtggtgcatg cctgtaatcc cagctacttg ggagactgag gcaggagaat 15660agcttgaacc tgggaggcag agtttgccgt gagccaagat catgcaattg cactccgtcc 15720tgggccacag agcaaaactc tgtctcaaaa aaattaaata taaaaaggag ggtatgaata 15780gacggtaaga gacatggagg acagaatggg aggtccaata tatgcctgta ggatttccac 15840gaagagaaca cagggactgt ggaagaggca atattcaaaa gatgatggct gagaaatttc 15900aaacattgaa gcaagatgtg accggaatcc tcaggtacag gaagcatcct gagtcttggt 15960aggaaaaatt aacacacaac caaatgagga cacatcacag gaatctgcag aacactgaag 16020gcaatggata tcttaaaatc aaccaaagag aaagactaat gatctacaca cgaagcatga 16080ttaaaccggc aactgattta tcatcaatgt accttctaaa gactgaaagg aaattaatgg 16140ccaaccaaga cttatactca ggtgaactat tatttgagag gaagaagtta atttcagaga 16200aagagagttt accactcaca gatcctcact gaaaagaact actaaaaatc tgaaaaggaa 16260acagaaccca gaaaaaagga ataggataga aggagacatg gggagcaatg aaattagtaa 16320acgtttaggt aaatccaaaa aacattagtg aaaatacaca cacacacact tatgagacta 16380gttttcacta aaaattggtc aaatagtggc aatttcatat agttctacct aacaaaaatg 16440tatttacaca tagtttagtt tgtgggggtt ttgtttgctt gtttgtttgt ttgttttttg 16500agacagggtc tcaccgttgc ccaggatgga gtgcagtggt gtaatcactg cagcctcgac 16560caccctgggc tcagatgatc ctcccacctc agtcttctga gtagctagga ccacaggcat 16620acaccaccat gcccagctaa tttttatatt tttttgtagg gacgggggtc tcgctatgtt 16680tcccaggctg gtctcgaact catgggctca agcgagctgc ccgcctcggc ctcaaaagtg 16740ttgggattac aggtgtaagc caccctacac ctggccaagt ttatctcatt tcctactgta 16800ctcccagtgc ctagaccagt gcttatcaca tggtagggag ggctcaaaaa catctattga 16860tgaagggact actttggagg aggtgttaaa agtaatgagg aactaaaata tcagaccgaa 16920gcaacctgga aatgggaaag gagagatcaa agtgaaagca ttcagcagcc tttgcattat 16980tcgggagaaa gatagggata ttgattatct atagactttg ttaggtcaga caagaatatt 17040aaaattttaa ggataaccca taaaacgaat agaaagaatg gataactttt aaactagtat 17100atggggaaga gagactaaag agcatccgat tgacttctag taacggacgg tggattaatt 17160gcatgcatta gcctctgctg ttctctgaaa tctcaataac gtggcattga agtgcacttt 17220ttacaaaatg cataaaccca aagggaaaat agaaaaggag acaaaaataa caacatttgg 17280gaagctggaa agcttccaaa tggtcaaacg gtggtgacag gcacacctga catgaatttt 17340gaagcagcag tggagaaagc caagaaggcc cctgattttc actactaaac tctccaaatg 17400ctctgacatt agtgtcaaca ttcttctgga agagtgggtg gcagtgggac tagaaatatg 17460gcagttggtc gagagtctgt ttgagaagca gctgttctct tcaactctac ttggctgggt 17520acccccaaag cagcagaaaa ccagatgctt attctctctg aaaagggtaa aagagtggga 17580ctccggcctg gagaaaacta ggtattgttg ggggccaggg tttttgtgct aagaaaaatt 17640aaggaaaact ttatacatta aattttgagg ctgggcacag tggctcatgc ctgtaatccc 17700aacactttga gaaggcaagg caggaggatc gcttgagccc aggagttcga taccagcctg 17760ggcaatgtag cgagaccctg tctctgcaaa gaatacaaaa attagccagg tatggtggca 17820cctgtggtcc tagctactca ggaggctgag gcagaaggat tacttgagcc cacgagttag 17880agaccgcagt gagccatgat catgctactg ctctctagcc tgggcgacag aatgagaccc 17940catctcaaaa ataaattttt ttaaaagact cctcagttca ccatctttcc ccatattgaa 18000aatggaacaa cattctccct gagaaatttg gctggcccaa ggaaataagc ctaaagaaac 18060tgacttttgg gggttccctg ctctccagat ggtccctcct aaattgaacc atggactcag 18120gatttcacat cagcttcttc taatttatgc agctttcttc taatttactg tggggttttt 18180tcggtgtttt ttgttttgtt ttgttttttg agacagggtc ttgctctgtc atccagctgg 18240agtgcagtgg tgtgatcata gctcactgca gcctcaaact cctgggctca agtgatcctc 18300ccacctcagc ctcctaagta gctggggcta caggcatgac caccacacct ggctaacttt 18360ttaatttttt gtagaggtgg gggtcttgac atgttgctca ggctggtctt gaaatcctgg 18420cctcaagcga tccccctgcc tcagcctccc aaagtgctgg gatttcaagc atgtgccact 18480gtgcctggct aagaatactt aaaggtgaag tgagaagttc acatgatgtc atatcaggag 18540gtacacaatg gctacttgtc ccactaatag caatccttag ttttttcact tcgtgaggtg 18600gtcactagca gacctcttgg ttggaaagat aggttatttc cctttgtaat taacaagtaa 18660cagaattggg atactttggc atcttaaaat accttggcaa cctgatgaac atagcataca 18720ttcaagattt ttgcctgaat taattattac attagaagag ttgcagaatg agaatgttct 18780aattctacta tttcttttat atatttcagc tgatgttatt ctatgaagaa aagctccctc 18840atcagtgaag atgaactgca cttctttcta aagaggcaat gtcagacggg cacggtggcc 18900tataatccta gcactttggg aggccgagga gggtggatca cctgaggtca ggagttcgag 18960accaacctgg ccaacatggt aaaaccctgt ctctacttaa aatacaaaaa ttagccgggc 19020atggtggcac atgcctgtaa tcctagctac atgggaggct gaggcagaag aatcgcttga 19080acctgagagg cagaggttgc agtgagctaa gattgtacca ctgcactcca ggctgggtga 19140cagagtgaga ctctgtctca aaaaaaaaca aaacaaaacg aaacaaaaaa acagttgaca 19200gtagttgttt ttgtagagtg aaaaatagag gtgggggttg agatactgct gctattttaa 19260actaacttgt agatctgttt gactcttttt taaaaaacaa ttttttggag agatgaagcc 19320ttgctatgta gcccgggctg ctctcaaacc cctggcctca agtgatcccc ctgtctcgac 19380ctcccaaagt gctggggtta cagacataag tcaccacacc tggctctgtt tgactcttta 19440tgtgcagttt gaacttttta actgaatttt ttaaaaggtg tgaaattagt ctttaaagga 19500acacaaaacc aacagagaag caagcaggaa gatacaagaa gcaaagaaat atcatagtaa 19560aatgaaaata caaaataata tgacataaat aaaacagtaa tcactatcaa attaaataga 19620ttaagtgagc ccacaaggca ttgtctctgg gactagtccg ctgggtcgaa gtcctgccac 19680ttattggttc tgtgaccttt ggcaaattat ttaacttctc tgtgattcca tttctttctt 19740ttgttttctt tctttcgttt ttttttttga gacagagtct cactctgtca ctcaggctgg 19800gtgcagtggt gcaatcacca atcacaactt actgcagcct ccacctcctg ggcccacgcg 19860atcctctcac ctcagtctcc tgagtccctg ggaacacaga catgtgccac cctgccaagc 19920taatttttaa attttttgta gcgattgggt ctcactatat tgcctaggct ggtctcagac 19980tcctgagctc aagtgatcct cccgccttgg cctcccaaag tgctgggatt acaggcatga 20040gccactgcac ccagcctcaa actctgtaaa aggggaataa tgataatact tgcctcatag 20100ggcttgtggg aaataatgct ttattactgt aggttaaaaa gcacacaaac atgcttgaca 20160cagtaggtgc tacatcagtt tactattgtt gttgttttca gattggattt tataaaaact 20220ctacttattt gcaatttata agaggcatac ctaaaatata atgtcagaga aaggttaaaa 20280agtggagcca gggaaggaat ttaagaagac ataccagacc aatagtaatc aaaagaaagc 20340aggtatgcaa tgtaacatca gaacaaataa gctttgctta tttggattga tagggataga 20400taggattgat agggataaag cagatcatta tttaatgatc aaagaagaaa tttggctggg 20460cgtggtggct cacacctgta atcccagcgc tttgggaggc cggggcaggg ggatggatca 20520caaggtcagg agtttgagac cagcctgacc aaagtggtga aaccctgtct ctactaaaaa 20580tacaaaaaat tatccgggtg tggtggtgca tgcctgtagt cccagctact caggaggctg 20640aggcaggaaa atcatttgaa ccggggaggc agaggttgca gtgaactgag atcgcaccac 20700tgcactccag cctgggtgac agaataagac tctgtctcaa aaaaaaagaa aaaagaagaa 20760gaagaataaa ttcactggga agataaaaca attctgaacc tgacaacata gtctcaaaat 20820acgtaaagca aaaagccact gagtcccaaa gagaacttga caaatttggt aggagatttt 20880aataccattt cctcaaactg atggaatcac agaaaaaaaa tataagaaca agtcagcaaa 20940cttgatatgg aaagaacagc atccccacct gagagtcagc agcctccatg caggaggacc 21000gaggggaagc cagggagcag ggcagcctga gccaaggcca agcccagttg aggacaggaa 21060gtggaaggag gcacctagga aggctgaaga tgcagaggag tgtgttccag aggcagtggg 21120aaaactggga gggaatagtt ctcgggatgt tggatagaga gaataagcaa agaacaatgc 21180aggaagaaga gtggggtggg aggccgtgac cttgctagag agccttatgt tttgggctat 21240gaccagggct gacagaagtg tgaggcatgg cgggttcaga agagagatca atgatgcatc 21300gcacatctct aaagctgatg aaggcagcaa ttggcactga ggccaacctg gttgggactc 21360agagaggtgg gacacatatt gtgtgccagg caccctgcca catcttggag agaataagac 21420acaaaccctc atctagtggg ttggggaaat gatgcagggc tcagtgtggc tagcgcgttg 21480gggtgggaag cagcctgggc acgcccacaa ggctctctgc cttccagaat ctggcccagt 21540tgggtttgaa aagatttaat gacaatgagc tctctatgga cctaccttgt gcccagtatt 21600tacctttctt atctgtaacg ctcaagtcaa tactctaagg taggaattgt tatcctcatt 21660ttatttacag agaaactgga gttcagagag attcttgccc aaggtcttgc cctgggccaa 21720ttccaggtct atctaactcc aaagtccatg ctcagtacac tgtctcctgc ttgggagcaa 21780agagaggact ttgctcctaa gaccagaccc ataccacaaa gagcctcaac acttaactac 21840tgggagaaaa caccgtccta agtaaaaagc caggtgctct tccccttacc ccagggggtt 21900taggatttgg gagaaagagg cctgatggaa tataggggta ccaggtccat gtgttaactg 21960atctgggtac aagaacagta actcaaggcc agccagggaa agaacagcca agccgaggca 22020cagctgccaa gtcatcacct ctcgttcctt gtcccaccat gccttgtctc agcacgaaca 22080taaccccctc agtttccctc ttctcctgtc tcctgcatag gcagaaccag ttctgagtga 22140tagccaggac agcaggtcct cgggtcctca ggcttgccac tacaacctgt ttaccctgcg 22200cagaccatga tccatcaggt cgagaaatgc cccactgcta

gatgtttgtg tcaaaaatgc 22260ctgccggtgg tgcaacagct gggtgggacc agtgacgtgg acttgagtgc cacaaaatgt 22320accgaacaca ttctgagtca acatagtgat ggtgtgccaa ctccagataa agattttcat 22380tattatttgt ttgggttttg tttatatgta ctcctcttca tcaccaaaga tttgaggcta 22440cagaaaatga aaattgaaat ggtaataaaa gagaggggct tgcagagagg accacaggat 22500ggggaaaata gaaataatcg cagaaaatca tgacccgatg aagagcacaa tcatagaagc 22560actaacagat gacaaggttg ccagatcttc accattggtt ttgggtttcg tgaaaacccg 22620cgaagagagg gattcagtca attacatatt ttttttcctt ctttctttta tttttatttt 22680ttattttttc ttgaaatgga gtctcattcc gtcaccaagg ctggagtgca gtggtgcaat 22740cttggctcac tgcagccttc acctcctgag gttcaagcca ttctcctgat tcagcttcct 22800gaatagctgg gactacaggc atgaaccacc atgcctggct aatttttgta tttttagtag 22860agactgtcac acgcgagaca gggtttcacc atgtttgcca ggctctcgaa ctactgacct 22920caagtgaccc gcccacagca gcctcccaaa gtcctggaat tacaggcatg agccaccgtg 22980cctggcttgt tttaattttt tattataaac aacactgtga tgaacatcca catagctaaa 23040tgcatgcatt ccatgatcat tttcttaaga taattttttt cttattattt gtgtatttat 23100ttatttattt ttgagacgga gtcttgctct gtcacccagg caagagtgca gtggcacaat 23160ctcggcttac tgcaacctcc acctcccagg ttcaaacaat tctcctgcct cagcctctta 23220agtagctggg atacaggcac aagccaccaa gcccagctaa ttttgtattt ttagtagaga 23280cagagtttcg ccatgttggc caggctggtc gccaactcct gacctcaggc aatcgcctgc 23340ctcggcctcc caaagtgctg ggattacagg cgtgagccac ctcacctggc cttaagatac 23400atttttgaag ggaagaacat tttctgtcca agtacattcc tgttcacaaa cccttttcca 23460taacactgga gacttcctgg ggaggttgga accaatggct tagactccaa aggaatgaat 23520gtaccataaa gctttcagca atcttctgtc aatgctactt gtatagactt ccagacaaac 23580attgaagatt gaatgcatgc atctaatttc actttttttt cctgaaatcc tcctgaaact 23640ataatgaaga aatgtttggg aaaagatgta gccaggcact gtggcttatg cctgtaatcc 23700tagcactttg ggaggccaag gtgggcagat cgcttgagtc caggagtttg agactagcct 23760gggcaacatg gcgaaaccct gtctctactg aaaaaataca aaaaatcagc caggcatggc 23820agacctgtag ttccagctac tcagggggct gaggtgtgag gatcacttga gcctgggagg 23880tagaagctgc cgtgagccct gattgtgcca ctgaactcca gcctgggcga cagagtgaga 23940ccctgtctca aaaaacaaaa aaaaaaaaaa aaaaaaaaag agacgggatc gggaaaagga 24000agaaaagacc acagcacaat tttggatgtt agaaagcaaa tggaccagtt gtaactgatt 24060tagccggcct tgggaatgct ggtcatgagc tggcagtgag aaaagatgag ttccagcctg 24120acttacaccg ctggattctc aaaaggcgaa gagatgatag tagggctcct gccagcccac 24180gctgcacctg tgtgtgcaat agaaacagcc tcccctttct caagacactc tacagccgtc 24240tgccagaaat ttgacatgat aaatatacaa atctgtcatg tctaccctgt gagggaggtg 24300gcctcagaat gatacagtgc acagcttgca cagccatata tgactgtcct gaattgggac 24360accagtttct actgaaagta gaggtgacgg aggaggctgt cataaaacat gaaagattgg 24420ttgaaactat ttaaggagca gttagagctt tagcttccct gtctcacctt agtagaagac 24480tggaagttta ttctcttgaa cgtcaggcag agttgagggc aggagacctg tactagcaag 24540aggattaaat caaagtatac cctctgaaat gctgaatcac cctcctgctt cttccacttg 24600gccccaggga cactgtattc aggcctttac cctccaggca gaagaatgaa agacatttct 24660taggggaata tgtccagctc aagaggtaag acccaaagat tttcacaatg gggttcccca 24720gctaaacatc tcagtggatc accctgcagt gacattcata gtcaataagt actacgcacg 24780tattggagct tcaaatcacc tttaaaatct cccattcttt tcttttttct ttttgagaca 24840gagtctcgct ctgtcaccca ggctggagtg cagtggtgcg atctcggctc actgcaagct 24900ctgcctcccg ggttcacgcc attctcctgc ctcagcctcc tgagtaactg ggactacagg 24960cacctgccac cacgcctggc taattttttg catatttagt agagacagag tttcaccatg 25020ttagccagga tggtctcgat ctcctgacct cgtgatccac ccaccttggc ctcccaaagt 25080gctgggatta caggcgtgag ccaccgctcc cagcccaaat ctcccactct taaacttaag 25140caaccaagga ttaccagtca tttgaggaaa gattctaaca tgaaagatag agacccaaaa 25200caaacaaata tttaaaaagg aagttagaga aaactgagaa tatacagaaa gaagaacatt 25260gaaaaatgcc attaataaca tcagagaatg aaaagattac attgattaaa caagaacagg 25320atagtataaa aagaccttca cagaataaca ataacaaaaa ctcttagaaa ttaaaaaaaa 25380gatagtagaa ataaaaactg aatagagagt aaagaaatag ttcagaaagg gagagcatgt 25440agagattgaa gtaagaacaa agataagaaa atgagaggat ccacgccggg cgcagtggct 25500cacgcctgta atcccagcac tttgggattt aatataaata tattaaatat tttaaataat 25560ataattttaa ataatataaa tatattaaat atattaaata tttaaatata ttaaatattt 25620aaatatatta aattcattca gtcaacaaat atttattgaa tacctactaa gttctagtca 25680tcatgctagg cactgactgt tcaagaataa acatagacaa gacttctgcc ctttcagaaa 25740ccagccaaga ttcttcaacc caaggcagct gtgaagtata acacatctct ggctcaattt 25800tctccatatg gtttggggtt ccatgaatga gtctgtggga agatttcctg tacgtagaat 25860gcatgcataa aggaataatt attctatggt tcgaaagaga aaaaaagtaa gatcaaagtg 25920tagaagctat gttaagaaag attccagttt aatgattttt gaaagagcag taaaaataag 25980accaagatgt ggaagctgta ttgagaaaga tccaatttaa ttctaataat ctttaacaga 26040attgcccaaa gatagaatga attgttttga gagatggtac agttagctgt cagtgggagt 26100gttcaatact aagcaagatt cactcgctca accattgagt gatgaccaca tgcaggcact 26160gtgctaggct ctctgtattt tcagtaagct attgttgtac aacaaagcac cttaaaacag 26220tgtcttagaa caacagtaaa tgtttatgat ctctcacaaa gtctgtgggt cagcagcttg 26280gctgtgtgtt tctggttcag agtctgtcat gaggttgcat ctgaaggctt ggctgaggct 26340ggaggatctg tttccaaggt gaatcacttt acatggctgg caagttggtg ctagctgttc 26400ggaaggggct tcagttcccc cctgtgttga cctcttcaca gggctgcttg cacgtcctcc 26460caacatggca gctatcttcc ctcagaatga gtgatccaag agaaatcaag gtaggtgcca 26520caatgccctt tatgacttag acttagaagt gaaataccat gacatagcct attgatcaca 26580catcagccac cattgtgagg gctagctacc acatcctgca aatacaatga tgaacaaaca 26640aatggtgtgc ctgagc 266566263744DNAMus musculusmisc_feature(750)..(850)gap of unknown length 6atatcgatca gctatgacct gattactaat tatggccact gtgctctaaa gagtagaaat 60aaggtgccca gccgaggtaa gagtcccctc agagaaagac tcttgggaga gtttgctgga 120atttaagtgt gcaggggtgt tagcttggtt ctgctagtat tctgtgcgcc taagcaaaac 180aaaacaaaac aaccctaaac agaccctagg accatttggg gggagaggtg aagcatgtgg 240aaaggcgatt ggtgggtgag aagtcaccta caaaatagac agcagtcgac aggtggcacc 300caggacactc ctggtcacac cacggggtcc tagagtccag atttgcccgg ctgtcatcag 360aaagtcgcag tggaaaccgt cagcagtact aaggaaatgt gtaaactgtg gggctgagag 420tcagagcctc cagcccgtcc tcctctgtcc tgactgcgag atctagactc tattctcatc 480gtcctcttgt gtgatagaag ggcaatggac actggccctc tgcagcatct tgtgacgatc 540cgagcatttg tgttcgtaca gactgcctct gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 600gtgtgtgtgt gtgtgtgtgt gtgtgacaca gagagagaca catataccga gatagagaca 660gacacagaga ccacttgata actaagtatg ctgtatactg tacatggtgc tgcgtacatt 720tttatctcaa ggattatagt ttacctgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 840nnnnnnnnnn gtgagtatca gctatgacct gattacgaat tatggccact gtgctctaaa 900gggatacaca tctttaatcc tgcactcggg aggcagacgc aagtggatct ctagagcttg 960aggcctgcct ggtctacaag tgagttcctg gtcagccagg gatacatgaa attctgtctc 1020aaaaaaaacc aaaataataa attaattaaa aataaataaa atttatttat tgtatgtatt 1080tgatttattt atttatttac ttacttactt acttattttt gtgacgggct tgcagccttg 1140gctgtgggga aagtggaaat aaacctggtg gaggaacaag tgggaaggag agtgaagaga 1200cataggcttc atcctggcac accatgagac aactcacagg accagagggc agagtggtgg 1260ctccttcccc tggccttcac agaaatcttc ctggatgaca ggcacctgcc ctacaggctc 1320atgatcagat gggacagatt cagctaaaca acacatgttg cacccagatt tgtccctgga 1380tacataaacg taccctaatt ggctgaaaaa ctgcatatca gcatatgtac taaacagtaa 1440agttagagct ggacctcgat gctggtcttc agatccaaat cccagggaat tttggggact 1500cggcttcact ccccacccta tttcccgccc ctgttccaca cttgggtgtc ctgaagtaac 1560tctgtaggca agttgactta aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680nnntaactat tcgacaacta ctatgacctg attacgaatt atggccactg tgctctaaag 1740ggaatcacag tagtcctcct gcctcagcct cccaaacact ggaattataa gtgggtacca 1800ctaccacatt gacttaagca atgaacctca gagctgtatg tgctaaaagg gttacggtgt 1860gtggattttt aaatctcggt ttacaaagag aaaccgagca ggagcagcca ctccagtggc 1920ttgagcagag gctaatccat gggtagggct gactcgcgag cctgggtgct ttgctcctgc 1980ctcactgatg attcctgacc tttcttctca tgtcttctgg aacacaatcg cccttctggg 2040acatggagcc gccttccttc cccaagaaac aattcatcgt tgttctgcta gcatcccaag 2100acatttgaca agcggcagca actgggctga aagctgagtc taggatgagg tagntatttt 2160aaagaccctg tgtttgttaa cttgcctttt ctccctccgc ccgtctgccc cccccccccc 2220tcttctctgt ctctctctgc ctctgtctct atctttctcc cccaccccct caaggtttca 2280cgtagcccac gctttcttca aactccctaa atagtacacg aatcctcctg cctctgcctg 2340ccaagtgctg taattacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncta 2460ttgataatac agctatgacc tgattacgaa ttatggccac tgtgctctaa agggctgcag 2520agatggctca gtggttaaga acactgactg ctcttacaga agtactgagt tcaattccta 2580gcaaccactt ggtggctcac aaccatctgt aatgggatct gatgccctct tctagtgtgt 2640gtctgaagac ggctgcagta tactcatata aaaataaata gatagataat aaatctttaa 2700aaacaaacaa acaaaagaaa tcaaagtcat aacagaaaga gaccactaag ggctagagag 2760atggctcatt caggaaatgc ttgcgatgca agcaagggga cctgagttta agattcccca 2820aagctaaatg taatgacaaa tgtttctaaa tccggtgctg gggagaagga ggcgggtgtg 2880gaagtgtgct ggccaggcat ccccgattaa tcagggggta acaggctcca gtaagagacc 2940ctgtgttcat aggtagctag aaggataaca cttaaacaat tcccagtgaa atccgttggc 3000ttacatatac acacagatac atgcccactt gtacacacat gaacatacac acgtgtggtg 3060gcacacacaa aagaaattgg tacacttgat acagcagtta cacccaacac agcagaagcc 3120atatgggtgg agcctatggt catttatttt ctcactccta agctacttca ctttaatttg 3180gttagtgtct ttttctaccc cgacagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300nnnnnnngta ttgacgagca tgttgacctg attacgaatt atggccactg tgctctaaag 3360aaatgttggt ccctttgaag gaatgctttc tgatcagtgc tttatttgtt tgttagtttt 3420ttttaataac ttctgactca cgagagctct ttccagaggt gtcgtctgag ggactctgct 3480gtaggccatc aggctggggg cagagacggc ttcgtggatc taactccttc agtgcctctc 3540agcacatcct ctacttgacc ttcttttcca cttgtacatg aacgcaggcc ctttgagaag 3600acactgggat ctacctgtgt gcattaaggg ctgcctttct ctctaatggc tggagctaga 3660tagagtcgaa ggcaacttgt gagcaacaat caaactctgg gagcagaagt gcaatgaaaa 3720gaaatgggaa gatggcctga catcacctca gcccaactgg cagaaaacca gtcttatgta 3780tgacccaagc caagagtgat gtgcttctcc aaacagaagg gagactccac ttcaactaca 3840ctcttcagga cacagactcc ccaacaggta acaggataga tgattaatat taatgagatg 3900acacatctca ttaatctact cttcccggga agacagccac ctctccagat gccagtacta 3960aaaagacctc gatgtatgtt cagattggaa tctggctcta cctcacccag cctgtgtcga 4020catgaagagg gcatgggaaa gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4140nnnatatagt agaacacctc tatgacctga ttacgaatta tggccactgt gctctaaagc 4200tggtaaatct ggcatcatct acctgcctat tatcattttc ttgtggtctg catcttgtgg 4260tccagtaact gggagtgctt ttgttgtttg ggggtcctgt tgctttggct gaacactggg 4320tgttctagat gacagactgg ggtacctgag gtttctgata tacctcccac ctcaaagctt 4380ctgtctgctt tgttcctttt caacacttgg attgactcca tccatattct taggatttgg 4440gtcctctctt ctgggcaaaa cagcctggca aattctggga tctccctagt ttgaactgaa 4500gtctctacac ctcttttctt agccaaacac agccattcaa ctccattaat tcttagctga 4560ttgcccactg tgttgttcgt ggatgcgagt tgttttacaa acaatatagc aaatgttggt 4620ccctttgaag gaatgctttc tgatcagtgc tttatttgtt tgatagtttt ttttaataac 4680ttctgactca agagagctct ttccagaggt gtcgtctgag ggactctgct gtaggccatc 4740aggctggggc agagactgct tcgggatcta actccttagt gcctctcaca catactctac 4800ttgacttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 4860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gattcgtcga 4920ngaacatcta tgacctgatt acgaattatg gccactgtgc tctaaaggct cccatcatgt 4980ggttgctggg agttgaactc atgacctctg gaagagcaga caatgctctg agctgctgag 5040ccactctagc ctccaattac agtatatttt gataaatgtc atagtagtaa gacaaggttc 5100agaaagataa aaaccccgga aaatatctga atttgccttt tcttgaatat gcacgtgtgt 5160aggccagaga acaatcttga ccatcaccct caggatcatc ggcttagcac tcgatcaggc 5220tagactgcca gcaactccag cactcaggag actgggcgag atggttcttt agtttaaaat 5280cagcctgggt tatagaaaga gactctgtct caaaacaata tattttgatt tttgaaaaca 5340tatatgtgtg tgttctaagt tatagtataa agtatgtttt tttatcatgg ctgtagtaaa 5400aattcttgaa tttcagtgct ttagacacat tagcagtaaa ctttatggca atgttaaact 5460gctgaaacat ttttaaacta taaaatgata tcatcagatt tgtgcgtttg tatgcgtgta 5520cctggaggtt tgcatgtgag gtgtattttt attattcgtg tctgccaagg tcacgcagaa 5580acatggagaa gacaagggat ggaactcagc attcacagat ttgacaaagt ctaactttag 5640ctaagagaag annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtacggca 5760tcagctatga cactgattac gaattatggc cactgtgctc taaagccttt aaaaatccaa 5820aattctgtta tgcctctcta gccagttaga gacagacagc agcaatgtgc aatgacatta 5880tttctataat aacaagtaca gatataaaag aatcctcagg aagctgtaga gaagccgaag 5940actcttcaag ataatgtgac attatctgaa gtgcaggagg gcacaccgag acgtgggctg 6000gggacagcac aacatggaga actagctcct ttatgtgctc aatcaaaaag ttccccaaca 6060tttggtctga tacagattga ctcatcccta ctcacccgtc gcgtgggcgt gatcctgttc 6120caggccagag atcgcacggc tgatgagtcc ttcaacagtg gtcagagctt gtggtaaatg 6180aaacatgaga caagtgagtg ctgacgtgct atcacttgtc tgtgtgaccc tcaacacttt 6240ccctgaactc tgcaggcctc cttctcctca tctataaagc tctaaactaa gcacaccgtg 6300gtctgtctca ctactggact cttaagagat ttaattagac gggcagtggt ggtgcacgcc 6360tgtgatccca gcacttggga ggcagaagca ggcagaattt cgagtgtgag gccagcctag 6420tctacaaagt gagttccatg atagccaggg ctacataggg aaaccctana aaccacnaaa 6480cnaaacnann acaaactaaa gagagagaga gataactatc tcnnnnnnnn nnnnnnnnnn 6540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 6600nnnnnnnnnn nnnnnnnnnn nntctatccc tcttagncaa cagctatgat cctgattacg 6660aattatggcc actgtgctct aaagagctag cagtcttatg tcatctccag gcttaaggtc 6720agatatgatc accaaccccg tgcagctggt ttgcacagcc caggctgcct ggccccttta 6780aacaggcgga aaggaaggag gcagattggg agggtggtcg gataagtctc aaccccactg 6840cagtgtagcg gcttccattt cttatgaata aagtgggtag gacctgcccc tcccctgaag 6900gtttgttgta gaaatgaaat ggtagtgtct aaaagcatga ggtgccgcat gcgcacaccc 6960cttgtaaaca gtcctcactg tgatagctaa actaggtgac cttctagact ggaatggaga 7020cccagtgtcc tcagaatccc aaaagggctg cttgctaagt tcctgccacc cccaaacact 7080cacttcaaag agacccagtg tcacaaaaca agtcttgcac attatagagt aaaagactaa 7140tgttcagaga gtgccagtgg ctgccccgga tcacgacaaa acacacttgc aggccagctt 7200tccattttcc acgttgctgg caccggcaaa gcctctgccc agtgttggga agctctttcc 7260catatgcatt ggcacagccg agcagctggg ctatggtctc tcttgaaatt caattagggg 7320ttctctttct gtcaccaaga gccagaagtc tcattgttca cacacggcaa actttcctta 7380cccaaacctt ggtttaatgt cctggtgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 7500nnnnnnnnnc atcgatatga tcctgattac gaattatggc cactgtgctc taaagatccc 7560tactcacccg tcgtgtgggc tgatcctgtt ccaggccaga gatcgcacgg ctgatgagtc 7620cttcaacagt ggtcagagct tgtggtaaat gaaacatgag acaagtgagt gctgacgtgc 7680tatcacttgt ctgtgtgacc ctcaacactt tccctgaact ctgcaggcct ccttctcctc 7740atctataaag ctctaaacta agcacaccgt ggtctgtctc actactggac tcttaagaga 7800tttaattaga cgggcagtgg tggtgcaggc ctttgatccc agcacttggg aggcagaggc 7860aggcagattt ccgagtttga ggccagccta gtctacagag tgagttccag gacagccagg 7920gctacatagg gaaaccctaa aaaccaacaa aacaaaacaa aacaaaacaa aagagagaga 7980gagattaact ataacttttt actcacatta aaacatgaaa atccactata attaaatgac 8040caaacacaag ccactaggcg tgacctggac caagctttga aagaaaatag gaaacatctc 8100agcttgtgaa cagctcacac acctcacaca cctgatctta aaactcacct cccttctggc 8160tggaagcttg gatctcnaaa tccacctcgg ggatcttgtg gtggcagagt ctgtctttac 8220cacttctctg gtcatgtcct ggacagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8340nnnnnnntcg tctggtanga acagcagtat cgaccatgat acgacttatg gccactgtgc 8400tctaaagtcc catctcacca tcgaagtcac atgtatgact ggtcccatag cttgtctgca 8460aatgcgccat gacactctat tacacttggg cctcagcttc ctagcagaac caaaataaaa 8520tcccacatcc tggcatgtgc aaccggccct ggccattcct gtcaccgtca ctccgttctc 8580tctcccttct tagccctggc catgctgacc ttctcctttt gactcgcctc ttctctctct 8640ggccttgcat tatcttcctt cggcttagaa ggctttgact actctgctta aatcagccta 8700tcacatcaca cagattgcat ctttcaaggt ccttattaag agttgtgtgt ctctcctcat 8760cagaatgata ttgaaaggga cagtttcttc tgctgctgca tccccaggac ctcaaccagt 8820gactacacag tcagcagctt atccatattt gctgacagag cctctgcaag tctctcctct 8880acaatgtccc tcaagcctta cctcaaatgt tctatcttga gtaaagcatt tatgtctatg 8940tgagcactag catgaagtct gaatcangaa agctattgag ggtctttttt tctttagtgt 9000cccaggcttc tttatttaag ttgatagtga cagcgtaatc tacatgagca ctcaacntca 9060tcatcatcca tcaccttgcg catctgatcc tcctcagcta atgannnnnn nnnnnnnnnn 9120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9180nnnnnnnnnn nnnnnnnnnn nnnntttttt attattagaa atcagcttga tcctgattac 9240gaattatggc cactgtgctc taaaggtttc catcccttgt ttagagattg aaaacattta 9300aacaattgtt agctgtgtac atgggtacac acgcgctatg gcatgtatgt ggagttatgg 9360catgtatgtg gaggtgaggg acaactttca ggagttggct ttctcttccc tttctaaggc 9420tcaaactcag gtcatggggg tttactctct gccacctctg accttgggcc gagactttaa 9480ggcctgtgat caatcaggag cagatggcaa gcgcctctct tcactaacag aatcttgata 9540tttccacccc actccctctt ccacaccagc actctccccc catgtgtttt cctgtctgtt 9600aaattctctt tctccccttt tggatttttt tttttagacc tgaaaatgag gtttctccaa 9660atctaagctc ccacccacgc ttttcaaagg tcttgtgggc tccacttccc ttctgtgccc 9720tcctgtctgc tttcctgagt gatgttccca agcctcaagt cacaagaagc tatcacttga 9780ccagctcttc tgagtgccca gctgaggctc acttctctgt caagtggaag tggctaaagt 9840gccacaagca ccgcccgtgt gggaagactc agcttgattc tatcttcatt gctttctgta 9900gaagtggaag gaacttgagc gaagtgccga tctttcttga cctgtgccct ctgcagccta 9960cccacaagct ggtgatagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt 10080ctttttcttt tntttagcgc aacagttttg accatgatta cgaattatgg ccactgtgct 10140ctaaagagag gtttgttctc tcatcctacc atttgggttc tagggtttta actgatgtca 10200tcaggctggg tgactttgtt cactgagtca tcttggtggt ctttcaatgt tttctgagtt 10260caggctctgg agtcgactcc ctgtgtttgc acgtggatcc cacacagtct gtgctgtgtg 10320gtcttggatg agtcttcgtt gtgaagattc tgtgagatag tctggctccc cttaggttcc 10380tcgttgaata ttagctatgt acgggttctc tctgtgtacc attgacccat tggtcctcat 10440cttgctttac ctaatcatgc ctggtccagc ctgtgctgtg cccaaaggtc agaactgatg 10500ggggatgagt

cacgtaggac atctcagcta cagttccacc tcctgaacct gcagactgag 10560tctctcaagt gccagcagcg tgggacaggt ccagaggtgc ttgcccactc ataccctctc 10620tgagaagacc tgtgttacct aagtttgttc aagggtggtt tccagtgacg ggctgtcaga 10680tatgcataga tatctgagtt gtccaggggt tcagaagttg gctctgagct aagagactgg 10740gtcatcgtac acacatgtgt ggctagcact cacgtatgca ccagccccta cagggagttg 10800tattgcctcc tgcgcgttat aatcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10920nnnnnncata ataattagtt taacagtcgt accatgatac gaattatggc cactgtgctc 10980taaagaacaa aaagaagaaa tggtggacaa ttttcggcag gagtcacgca gacgtcttat 11040cagctgccat gtagaactga tgagaattga tctacagact gatcctgtga gcaagcagca 11100gtgaaagaaa gaaaacagtt attcaagaac atctatgaaa attcagtaag caatgagggt 11160ctagattctt gagccagccc tatgcatggc accacagcct ctctgactct cccatgccct 11220cctcatgtgc acacaggctg ggtgaggtag agacagattc caatctggac atacatcgag 11280gtcttcttag tactggcatc tggagaggtg gctgtcttcc cgggaagagt agattaatga 11340gatttgtcat ctcattaata tttatcatct ttcctgttac ctgttgggga ggctgtgtcc 11400tgaagagtgt agttgaagtg gagtctccct tctgtttgga gaagcacatc actcttggct 11460tgggtcatac ataagactgg gtttctgcca ggtgggctga agtgatgtca gggcatcttc 11520ccatctcttt tcattgcact tctgctcccc acatttggat gttgctcaca agttgccttc 11580gactctatct agctccagcc attatagaga aaacagacct tatgcacaca ggagatccag 11640tgcttctcaa gggctgcgtc atgtctagtg gaaagaacgt cagtataaga tagcatgaga 11700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgctgcggt ttttanagac 11820atcagtntng accatgatta cgaattatgg ccactgtgct ctaaagttga tggttatatt 11880aagtgcttgg caccatcaga ccttctgaat aactctggcc caaggttcct catttttcct 11940ttccaaaaca ggttttgtta cctgctccaa atcacccagt agaagtatca ggaaactagg 12000atgcaaaccc aggtctgggc ttatgacaca cagaggacgc cttggaaggg agggagaagg 12060ccagagcagc tgggaccacc cacagcatgt tccagtccat cccacgggaa ctttacccat 12120tctgaggcag aggggtttga cttgcagacc tgaggcagag gctacagcca gtatgtgaaa 12180tcttttggaa ggaatccctg aactactcta ctctgagatt ccctccttag ggaggctcta 12240agtctccaag ggcagggaaa attcattcca gagactgcag cctcctgtgg ctggctgtcc 12300ccataaaacc actggggtcc tctgatgggt tcatttcttt gccccagcga ccaccagctt 12360tgtcatagac aggcaagcca cccatcaaga ggctcctggg caaagcctgt tccaccttgg 12420ctgccaaggc tcgttcatat cccgtggcat ctattataca gctctggagt caacagattt 12480atccataggt ggagaaaact ccggcgcaaa catgagtact ttacagtggg acagagtggc 12540ttacttttgt atagataaga caaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12660nnnngatttg agacagcagc ttcgacctga ttacgaatta tggccactgt gctctaaagt 12720gggaattgaa ctcaggacct ctggaagagc agctagtggt cttaaaccgc tgagccatct 12780ctccagcccc agttgccaat gtttttgtta tgtgtagatg ctgttattca gcctcccttc 12840cagagaaaac ctcaccatat accatgacca ctaaaagggg tcatgtagtt cagtgacgat 12900atgtcttccc agagtggtga gggcctatgc tcacttgcca ggatgggaga gctgggtagc 12960tgtgtagagt gggctgagta cggtggccag tttttgcact tggaaggtca gagggttgag 13020agttcaaaac cacagccttg cccatatagc aagtttgaag tcagcctggg ctatatgaga 13080atatctaaaa acaaaacaaa acaaaacaaa acaaacaaac aaacaaaaac cacagatcat 13140ctctaaaatc ccattatgga aagtgagttt tcaagctaag aggaactttt gagaccatct 13200atttgaggtt ctaccatgaa cttccttcta gataagggcc tgttgagagt ggaaggaatt 13260tgctgtgtcc tttatctctc agccttagat gcaaggatgg accttgcgcc caggtctctg 13320catttgngac tccaggcagg aatgtactct gcccatctcc cttagaaact ccccaactcc 13380ctaaaaccag cttggaatct gtctaccaga tcagggagtg ggctggatta caagctggtn 13440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gttgttgctt tttntagcta 13560cagctttgac ctgattacga attatggcca ctgtgctcta aagggcgcca taggtctgta 13620accctgtaag tactggctct tctcaggctg tcacagtggc catggtggga caagtaaggc 13680cagcccaggc tctagccagt cctggacact gtccctctgg ccagctggct agtgcagaag 13740gcgctgggga gttaagtaaa ggcagggccc gcagtggaca gacaagcagt gttcattggc 13800tggccagtca ttcagtaagt ggggcggttg ctggtcacag gatttggggg gtgggttctg 13860cccacactta gtaaaggctc gagggagcca cttgtctact ggggcagccc ccctgaggct 13920ggggagctta ctaaccccaa cagtgccagg agaaggggaa aaggtgagtg tgctagtaac 13980cagctggggt catccttctt tgaccctcca cgtggtgttt gctcagagga attgttgtca 14040gactccctgg cgcctagagt ataaagtaag agtggctgct ctctgtgccc agaaccggac 14100aggtgagctt acatggcaca gggatggtgg ggngtgggtg gcagaaaagg taggagagag 14160ctgcagatca agctagcaga aattgtgtgt accagtcctc attcctaaaa gcngctcggc 14220agacagaaga caggcgaacc gaggagctag attgcatttg agtggtgggt catctctcta 14280ggcagtctga gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 14340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntacatcat 14400cgcttgacct gattacgaat tatggccact gtgctctaaa ggctgatcag ccccacctcg 14460ctctcttact gcttactctc ttacttgtct ccctcttacc cctcactctc accttctctc 14520ccctctcttt gtcttctctc ctctcttctc tctctttgnt ttctcctctc ttctcctctc 14580ctctcttctc tccctcttac tctctttctc tctagtctct ctctctctct tctctctttc 14640tccctgcatt tctataataa agctcttaaa ccatagagag tctctgctca tcaaggctgc 14700atgcactctc gtaggtattg ggaacatctt ccctcatccc tctctcctat aaccctggtg 14760gctttagcaa agtagctcca gggtccccag gtagggctgc ccctggccgt cccctgaaga 14820gagggtcaga ggcttggatg cccatccagg gatgagtgga aaggtagcag ccctcccaca 14880cctgacaaac cagagaatan gtaaaactct ggtggggcat gggtctatct cccccctctt 14940cattccccag gccccctttt ttagctccca cacaatactg acttccaggc agctaggatg 15000aaagtcttag agcccatgct cacagtgata catctattcc acaaagccac acctcaaata 15060gtgcacttcc tgggccaaca tatcaaacca tggcactgag atggtcttct ctgcccacnn 15120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 15180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gccgtggttg nnngacatct 15240ataagccatc attacgaatt atggccactg tgctctaaag tttattattc cactgcactg 15300taacttcgtc tcagtgggga actgttgtac atgggggaac tgttgcttct ggctcacaaa 15360ggtatctgcc atcacattta catcttacat cacacgggca cataacacat aattaaaaag 15420aaattccttt aaaaatccaa aattctgtta tgcctctcta gccagttaga gacagacagc 15480agcaatgtgc aatgacatta tttctataat aacaagtaca gatataaaag aatcctcagg 15540aagctgtaga gaagccgaag actcttcaag ataatgtgac attatctgaa gtgcaggagg 15600gcacaccgag acgtgggctg gggacagcac aacatggaga actagctcct ttatgtgctc 15660aatcaaaaag tttcccaaca atttggtctg atacagaatt gactcattcc tactcacccg 15720tcgtgtgggc tgatcctgtt tcagccagag atccgcacgg ctgatgagtc cttcaacagt 15780ggtcagagct tgtggtaaat gaaacatgag acaagtgagt gctgacgtgc tatcacttgt 15840ctgtgtgacc ctaacactgt ccctgaactc tgcacgcctc cttctcctca tctataaagc 15900tctaaactaa gcacaccgng gnctgtctca ctactggact cttaagagaa taaattagac 15960tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natatacgat atgatcctga 16080ttacgaatta tggccactgt gctctaaaga ccctacggag ggcagtagat attgaagaaa 16140gctattctgc tttaagctag aacacctcag tgtggttgac atgaagggta tggagagtta 16200ctgcccgggt cctggcactg tactgctccc cctgcccctc cagagattac ctgctgcaac 16260tgcttgatgc tgctgctgtt gcccattttt tccaggtgag ctttgaaggc ctggatgctt 16320gcagccccgt ctgagaaccg gcgcacgggg gagaaacgct ccgtagggag atgcacggtg 16380ttggagtcct tgtaggtgga gctgaatata tgggaagaga cggttagcag ccaggactcg 16440gggggttatg accaatcaca cgggaccatg ggtctacatg aatacagaga cagtggatgc 16500atgaggagcc ctgcacatgg tactccgtgt cacttcctgg agtggctgtc cccctgagtg 16560gactacaggc cctcatctcc cacctccact tacactcagt tggtcctact tcctcagcct 16620gctgcagtcc tggggtcaac tacatccggg tcttatataa acatactaga gcagggtgcg 16680aagtccgcag acggccagga ctggctgaac cacacaggct tggctcttat agtcacatca 16740tttatttcta ttgtagntgc atttggggag cggtgtgtgg ataatgttgt gcaagctatg 16800ggttctaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgtggtattt 16920gcgatcagta tgacctgatt acgaattatg gccactgtgc tctaaagtca agacgggaat 16980tacagatagg tgctaggaat tgaacccagg tcctctggaa gagcagctcc tgagccatct 17040ttccagtcat ggtctttatt attctttaca tcttttacca gtgcacactt gaatcatatg 17100ttggtcattt gtaacttaca ggttggctga gttttacaca ccttccaaat gctgacgttt 17160ttattataca ccatcagaaa attacttgag tggtagattg agaaagttct ctaaatacat 17220gtagaatatt ttcccattct tttgattctc cctagtgact gatgccctta atatacattc 17280agcaattact tgggatgatg gtgctttata aaactctgtt aggctaaggt ggcttgagag 17340tttacagaac tgtcctgata ccttagttgt tttctctcag ttgctgttat gtctcagctg 17400cgttgggtcc agaattccca tctgatggct tgcatgtctg cactgagatt tcccatctgc 17460tcacccattt aaagactgtt ttctcatttt gtaaaaatat ttataaatgg tgttttgaag 17520gtttgtctgc tgattacaac attctgctca ccacaaactt gctttttggt aataacctgt 17580cccctgcctc cctctctgtg aggaccgaca gggtctcact ctgtatgtca gtctgattta 17640gagcttgcta tgtagcccan actgatcaca gactcagcac nnnnnnnnnn nnnnnnnnnn 17700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17760nnnnnnnnnn nnnnnnnnnn tgtgtttggc tttgcaaact gctatgacct gattacgaat 17820tatggccact gtgctctaaa gttctcgcca agtgtcttca ggtgggtttt ggccctggtg 17880tggtactcgt tcaactangg ttgctcttga gctcagccag gcgctgggcc aggctctcgc 17940gcatctgttc gctgtggggc gctagctgtg tgcgcagaga gtctacgtgt gtgcgcatgc 18000ggtcgcgaaa ttcctcagcc acaggggaca gtctcccttg cagctcctgc agcttctggc 18060gcgcgctctc ctgcagctcg gcgcccagag gcgccacctt ctggcggtag agctccacat 18120cctctttcca tttcttctgg aattcgtcca ggtagggctg caccttctgt ttcacttcct 18180ctnagtcctt gttcatctcc tgtctcaccc aatctgtttc tttctccagg ttatcccaga 18240agtcccgagt caatgggccc agccgttcct gcagctgact aacggttgaa cccagagtgt 18300cccagttttc caggagattc acgctagagg gggaagagag cggctgagag acgagcccag 18360gcatcatgcg ctgcccctgg tctgcagatc catgcacatg atagctgngt gccacctgtc 18420ccctaatgtg caggggctat ggggctaggt tccccgaaca tgcgacccgt ctaactcact 18480ggcttctctt tacatttntc tgtgacacat gcggggaggg agccagnnnn nnnnnnnnnn 18540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 18600nnnnnnnnnn nnnnnnnnnn nnnnnnatta aagttatcat ctatgacact gattacgaat 18660tatggccact gtgctctaaa gaaatccaag taggccctca ctccctcttc gctctctcag 18720aatgcgtttt tgagtttgct ggcaggcaca ttggcctgtg cattgtctga aagactcagg 18780gcagcgtgga gtgtgagcca cgtttgtggg tgacatgtct gagtcacagc gctttctgcc 18840ccttcccagg aatagcccta atgcctttcg tagctcacga tgctgccgtt cccccctttt 18900gtgttttagg attctatttg tcctcctcag ccctccccac ctcttcaggt tgcctgtgaa 18960aaccaaccag ccctcctcac ccaccagctc cagaggtaat gaactactta gcctcttcct 19020gcattgttgg ctggctggac ggtgtggctc ccttgtctga cgcatgggga cgcttgactg 19080acttgagcat ttgggatagt cttctagaag aacagggaag ccgagttaga gtttgaaggg 19140cttactagaa tgctgactat ttcctgagct ccatgaacac agtggcctga catccatcga 19200aggccaggcc agtgagtgct gagttggtgg caggggaagg actggtagac tgaggtgtgg 19260gcaagctgtg gtggcctgct accgcagttg tccgcccana ctacanactg tctgtctgca 19320tggattgtct gggctgatgt tactgcagat gttctgtcac tcagaagcnn nnnnnnnnnn 19380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 19440nnnnnnnnnn nnnnnnnnnn nnnnnnnnct atcaacaatc cgctntgaca ctgattacga 19500attatggcca ctgtgctcta aagatttatt tttatatgag tatactgcag ccgtcttcag 19560acacacacta gaagagggca tcagatccca ttacagatgg ttgtgagcca ccaagtggtt 19620gctaggaatt gaactcagta cctctggaag agcagtcagt gttcttaacc actgagccat 19680ctctgcagcc cacgactttg atttcttagg taaaaatctt tatgacattt atcacctctg 19740gttgagtctg taggccataa atcttttcct atcctctgaa aagggactgc tggctgtctc 19800catctattat tcaaggcgat gctttaagct ggacatccag tgctcacctg taatctcaac 19860atttgggagc ccgaggcaga agaattgaaa attctccaac ctggtctcaa tctcccaccc 19920caaaagtaat attttaccat ctgtatattt gaaagagcat agtgaattgt aggttcatgt 19980atgcttaccc ttggaagtgt aaatgaaaga tttctaaaca ttgcagatat aaccttgcct 20040ctaagatact ggtcattctg aaatcatttc ttcccggggc ttgttttctg tatacagtca 20100gcatatctac aattatgctt ttccctgtat gtcacctcaa gtatccgatc caagatgctc 20160tgntaaatca ccgtgtgcca tgtttgcatg caagcaatgt acaagtacag gtgnnnnnnn 20220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 20280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttatcat caatatgacc tgattacgaa 20340ttatggccac tgtgctctaa agatctagcc ctatcagctc taccagcccc ccagaccccc 20400cactagactg aggacagctg tttgtctgtt gcagagtgtg agcacttgat ccgccacatg 20460ctggtgttag atccaaataa gcgcctctca atggaacaga tctgcaggca caagtggatg 20520aagctcggag atgcagaccc caactttgac agggtaagcc ggacccttgc ctcagggaac 20580tcctgattaa ctccttctct tcttcagtgg ttgtgatctg cctaggacct aaagagatga 20640gcagtaggaa ccagctgctg gctaagacca gtggcaggtg aattcacagg gagccgcggg 20700atccagcaag gctgtggaga cagtgatact gggcattacc acagaaatca tagtttgctt 20760ttgacttctg agacagcata ctcccagttc tgcaggtcac tgccaccaga gtgcctttct 20820tgtcttgagt gcgctccctt caacagcttc tgtcacatac tgctgcaccc cattatgatc 20880actctcgtac ccccacgagc tcacgaagca gcccctgacg gttttggtca gcccatagct 20940gattgccaga actgtcttgc ttagacttcg tatgacaccc acagaatctg cagcagtggg 21000gccatacaac agctttggca atgtgtatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 21060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 21120nnnnnnnncc cttcgtcagc atcatctatg acctgattac gaattatggc cactgtgctc 21180taaaggaaga tgatgtcctc tgttgctgtc taggctgtgg gtctcagtac tagctcaggg 21240gactatctta gttgccccag agcgcctcct tcatcctcac cttctttgtg aatgccctgt 21300ctataccttg ctgcttgcag gttctcatag atagggctca tgtaggggtc agatatcacc 21360cagccatggg ccctgctcct atctcaggat tactaacgat aggtccagag ggcaagagga 21420ggcctgtgga ggagttgggg aggggcaagg ccttgggttc catgtgacct ttgtccttct 21480ctcactaatc cctgcccagc tactgcagag gagactgttg ggatcacagg tggaggtcag 21540tgtgggggca tgtaaccttc actctgcccc ctcccacccc acttcctccg atgctccaca 21600gtggcaccac agattcccca ttccattcta ctggaaggct ttcaggcggc ttctgtgaca 21660gagcctgtca ctggctctag gctgtaaagc aggcctgagt ccaagcttgt ccctgtcttt 21720ttctgtctca ccgactcatc tagtgagccg gtagctcacc cttggcggcg gcagtggacg 21780agaaaagcat gggcaatctc agaaggattt ctcaactcct ctggcagatg gctgcatgga 21840cgttctgcct caggctctga tctggacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 21900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 21960nnnnnnnnct tgattttggg atcatcttcg acatgattac gaattatggc cactgtgctc 22020taaagagtcg agctgaggtc tgaaccccga agtcataatt caccttcatg acacggnagg 22080cattccctta tgctcctgga actctggccc ctgcgtaagc cccccacatg cccccataag 22140agaagcatgg tcagtagtca cgtaggcaat ggtccaagct tctgaccttc aggctgaact 22200cctccccagt tacctagcaa cagtaaagac cataaaaagg gctgatcagc cccacctcgc 22260tctcttactg cttactctct tacttgtctc cctcttaccc ctcactctca ccttctctcc 22320cctctctttg tcttctctcc tctcttctct ctctttggtt tctcctctct tctcctctcc 22380tctcttctct ccctcttact ctctttctct ctaggctctc tctctctctt ctctctttct 22440ccctgcattt ctataataaa gctcttaaac catagagagt ctctgctcat caaagctgca 22500tgcactctcg taagtattgg gaacatcttc cctcattcct ctctcctata accctggtgg 22560cctttatcaa agtagcttca gggtccccaa gttaggcgtg ccctgggcgt gctctgaaga 22620gaggtcacaa gctggatgtc ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 22680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 22740nntggtataa gagatcagta tgacctgatt acatattatg gccactgtgc tctaaagatc 22800cagtaaaaaa cgccctgttc ctaagccagt gtaagaggca aagaaaggag ggcccttggg 22860cctggctggc cagtctgtct agtttaattg gtgggttcca cgccaatgag agatgctgtc 22920tcaaaggagg tggacaagtt ctctgaggtg acaccaagac tgtcctccag cctgacacac 22980aggaaagcag caggcttgct tgcacagcgc ctgcctgccc ttcatggtgg ccagccttcc 23040ttcctcttcc tgctcactct agtgaagacc ccagggtagt gaggagaagc tggagaggaa 23100acaaggaacc agcagacagc tgggggtgct ggccacacac tccccaaccc tggcagcaca 23160agccccatca ttgggtgcta attaaatccc cctaatgagg aaaacggatg caggagctgg 23220ctcctccggg gcctccccgg ccccacgccc ttgcctctcc atcggactta tcgctcgtca 23280caggatgttt acaataattt ttaatttgtg caattattaa tcacttttct atctccctca 23340tttgcataat aattagacat ctcactgttt gggatgcctg ggaatgacag ggtttaaatt 23400gaatttagat ttaatattct attttctgct ttttcccttc acacgctata aagccagatt 23460ggcaccagag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 23520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttatctatac 23580gcattacact gattacgaat tatggccact gtgctctaaa gtactgctgt gaacagatac 23640catgaccaag acaactctta taaggacaac atttaagtgg ggctggctta caggttcaga 23700ggttcagtcc attatcatca aggtgagaac atgacagcat ccaggcaggc atggtgcagg 23760aggagctgag agttcaacat ctacaattta aggctgctag cagaatactg tggggaattg 23820caggcttgtc tccagtcgag ctgaggtctg aaccccgaag tcataattca ccttcatgac 23880acggtaggca ttcccttatg ctcctggaac tctggcccct gcgtaagccc cccacatgcc 23940cccataagag aagcatggtc agtagtcacg taggcaatgg tccaagctta tggagccgtg 24000tagtcccagc ccctacaagt caggccagga gggtcagtgc agccttgcag ctaacctggt 24060ctacattgta agtcccagtc taaccacagc tacaaggcaa gacacagcct taaaaaacca 24120gcatgccaga ttgaatgcca aagtgaatga aatggctgat gggtggcaga ggctcttcat 24180gggcaaggtt ggcattggtt ggcaaggatc agcatcagca ttgtggatag tggggtgctt 24240cctctgtcca ctggactcgt tgctgccaag gtcactaaca agctcttgtt ngataaatca 24300gtaggcattt nttatcntta tttttntgga tggctcannn nnnnnnnnnn nnnnnnnnnn 24360nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 24420nnnnnnnnnn nnnnnnnccc cttttttgat cactacgacc tgactacgac ttatggccac 24480tgcgctctaa agagtggggg ccaatcttgt ctcactaaaa ttttgacttg ggcggcagag 24540actcactggc tgcaccacgc acaaagacaa ctagaggaag atctgcactt tggttctgca 24600aaccacatca ttcagaaaac acaggatgac tctgttctca atgtgagcaa tcagagactt 24660tctacatcca gtttgaaaag actggtttga gatggtggct catcccggat tggacctgca 24720tgtagaacct cctgcaagac tcacctcaca cttcctaagg agacctttgt tggcagctcc 24780cagaatctgc aatgagcatc catttctcct tcagcctttg aaatcacaca taaagataag 24840gttatgcaga taatctgaat atttctggga atattgaaga atcctatagt cggttataaa 24900gggatattgg aatctggaag aaaacgggtg attccgaaag gtctgaactc tgccattatg 24960cgtggagaag aacagaactg aagaactgcc aacagggtta gtctcctgcc tcatttaaga 25020acgttgtgga gaatcttcat ctgtaaagac aactttctct cctgatcctc acgccttctt 25080gcctcaagtt gatgttgatt atccgcacgt taannnnnnn nnnnnnnnnn nnnnnnnnnn 25140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 25200nnnnnnnnnn nnnnntttga atacggatgg ccagctccat gtgctctcag gtgtgtatgt 25260gtgtgtgtgt gtgtatgtgt gtgtgtatgt gtgtgtatgt gtgtgtgcgc gtgtatgtat 25320gtgtgtatgt gtgtgtgtgt atgtgtgtgt ctgtgcgtgc gtgtgtgtag tgtgtgcgtc 25380gtatgcgtgc gtgtaccgcg tgtgtgtgta ctatgtgtgt catgcccgcg tgcgcccccg 25440tgtgcccgcc cgccgccccc cccatccccc atccccatcc cctgcctata cccggccatc 25500cccccacccc ctctccacac cccagtctcc ctcctcccac ccccaccccc cgacccatac 25560cccacccccc

ccaccctccc cacccaactc cccctccccc ccttaccccc tcccactccc 25620cctctttttc cccccaccac actaacaccc ccctgccccc ccccctgacc tccccccctc 25680cctccccccc accccccgct ccccgcctat tttcccctct accccccccc cccgtcccca 25740cctcgatccc cccccccccc ttcccctggc gccacccccc ccaccccccc ccccttgaaa 25800cccccacgta cccccccccc tcccccccat cccctcccca ccccccctcc ccaataacta 25860ccctcgcctc cccccacctc tccacccctt cctccctacc cccaattgac ccccctttcc 25920ccccctcctc ccctgcctca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 25980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 26040nnttagcaaa catcttgaca ctgattacaa ttatggccac tgtgctctaa agaggcatgg 26100aggagggggc attatgtggg tatgtaggtg ctcttgctaa ggacgtctta ngcccaaggt 26160gtcccctctg ctgtgccatc ctctggctgt gcccggatga tatcagaaca gagggggtgg 26220agctatggcg gaggaggaaa aggcagatgg ctcagatggg aaggcctggc tgctgtgtgt 26280tccttagatc cagagaggcc actagcctca ctgttcacat ctccaggatt ctgtttggac 26340cactccatgc aggagacgcc ctcaccccaa cccccgcctt catcccatat gtcttctcag 26400acttgggcca tctcctccac cagttggccc aagcttttga atgccaatat gatttggggt 26460tttgttttgt tttgtttttt gaaacatttt ttattctggg gggttttaga tgagaactat 26520ctggtaaaat ttatatgagt gttcaaaata aaactaaacc ctgaaattgg aaatactgat 26580cccaagcatt tccgatgaag gacagtctgt ctgcactgga gttcggtggc agaggcagcc 26640tagcaagaga agctgctagc ttctagcagc acagctctac agcatggcca tagggagggt 26700gctgagcctc agagaaagct tcgcagatgc ttggatggaa ggaagctgtc tgggtaggtc 26760tcagtactgt ggttatgctg accttgctct agggaccgga gactgaaacn nnnnnnnnnn 26820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 26880nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tggccactgt gctctaaagc agcccatgga 26940atgaccccag cccatggaat gacctcagcc catggaagga ccccagccat ggaaggaccc 27000cagcccatgg aaggacccca gcccatggaa ggaccccagc catggaagga ccccagccat 27060ggaaggaccc cagcccatgg aaggacccca gcccatggaa tgactccagc ccatgggagg 27120accccagcca tgggaggacc ccagccaatg gaaggacccc agcccatgga atgaccccag 27180cccatggaat gaccccagcc catggaagga cctcaggcca tggagtgacc ccagcccatg 27240gaaggaatgg tgttgcccac acttaggctg ggtcttccaa ctcccttaac ctaatgtaga 27300taatccctca ctgatgtgac cagagatttg tttccatggt gattctaaag cccatcgagt 27360agacaatcaa gattagccac cacagtctct gaggacaaga tgcctgcatg cagaagtgtc 27420tctccctatg ccatctaaga tggctgccag ataactgtga acttaattat aatgtcatcc 27480ccacactaaa cgacatactc cagtgtcttg acagttgaca attgctgtga tgacaactag 27540aaagaaccat ccaaggagcc aaaggaggtg gccccagaat tcctgggaaa gctctatcca 27600ctcncagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 27660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng attatcatgt 27720tgacctgatt acgaattatg gccctgtgct ctaaggggca ctccatcgct tctgcggggg 27780ggaataaagt cgcttacctt tatcctcgnt gtgcctacgc ttacagttca aagacctgct 27840ataccaacct cccctcggct ggtgcgatgc gtggttatgg cgcgccacaa gtcgtatttg 27900ccggtgagtc tatgcttgat gacgccgcga cagcgttagg tattgatcct gttgaaattc 27960gtttacgcaa cgccgcccgc gaaggagatg ctaatccgct cacgggcaaa cgtatttaca 28020gcgcagggtt gccggagtgt cttgaaaaag gccggaaaat ctttgaatgg gaaaaacgcc 28080gtgcacaatg ccagaaccag caaggcaatt tgcgccgcgg cgtttgcgtc gcctgtttta 28140gctacacctc taacacctgg cctgtcggcg tacaaatagc aggcgcgcgc cttctgatga 28200atcaggatgg aaccatcaac gtgcaaagcg gcgcgacgga aatcggtcag ggtgccgaca 28260ccgtcttctc gcagaggtgg cgagatctgt gtgggtgccg gtcagcgacg ttcgcgttat 28320ttctactcta gataccgacg atacgccgtt cgatccctgc gcattatgct cacgctagag 28380ctatgttgcc gcgcctgcgc tgcgcactgc gcactatgat tcnnnnnnnn nnnnnnnnnn 28440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28500nnnnnnnnnn nnnnnnnnnn nnaaattaat gtggagactc agcgccaacc tccatgngct 28560ctccagagga agaggaactt atgagggtta gaagggggct ggggggctgt gatcaggata 28620taaaatgaat acatacatta atgagaaaag tgatattata cttctaaagt gttgtaaatt 28680aatactggtt cttttagaat acaggagttt aagtttgcca atctgccttt cacctctatt 28740tggccgtgtt aattgctgtc agcacagaga attccgcgnn nnnnnnnnnn nnnnnnnnnn 28800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 28860nnnnnnnnnn nnnnnnnnct tcgtgccgct tctccctggt ttccgacacc ttggccggtt 28920tacccgggaa acactggtcc cgcctttttc tccccttctg ggaaaacggt gtgggggctt 28980ttcttaataa acttcaacgc ctggtgaagg tattcttaaa atttcggggg agtaaggggt 29040ggtttcggct tttccaaact ttgggcttgt tggttcaaca caaacccccc cccggttcag 29100aacccgaccc cggtgtggtc cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29220nnnntttggn atcgtgactg gccaactcaa tgtgctctaa agccacaatc tccagcaacc 29280agcccaaagt cgggcccata caagacattt aatatttgtt gaattcaccc ggaggccagc 29340ataagcttct aaagcatgcg ggagtaattg aaactcatta gagtttcttt gggtcaaatc 29400aatgcataga ggtattaaaa ggttatttgt atacattcaa accatacaca atccttatag 29460accactagtg gcaagtctgg gagaggttca tggattatat gcacacacat acacacatgt 29520cctcgcacac ataagtaaat gtagttttaa agttaaagga agatattata agatttaggc 29580ctaaattgaa tctttgcaat gattggtttc tttttctttt tccttttttt tttttttttg 29640gggggggggg ggggggggaa gcaagggttc tcaggataat tttggctggc ctggatctca 29700ctttattaac caagctgact ttgaacttac cgagacctac cttgctctga gtgagtgtga 29760gctggcacct tctggatgcc attggtttct tctttgggac ctcttaggac agacctggtc 29820cttgacaaga actttgcagg ctgtggggaa caaaaagctt ttgtgctttt tttttaacct 29880ccctttaaaa tccaagggct ttctaaattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30000nnnnnnnnnn tctccttttc cttagaatac tgtatcagcc tgattacgaa ttatggccac 30060tgtgctctaa aggaatcttc ctggtttctt tgccacactg accacgaatc catttcactg 30120ggatgagttt atatcatttt ttagaaagac atatcaaagg tgagaaggtc agtacaggtt 30180tgataaaaac ctatcaaact tttatagtca caagtttgac ttcccctaaa tggtacccgg 30240aagcctcttc cagcagactt cccaccaaaa ggtatttata gcaataaaag tgattgaaat 30300tgaaacgttt ggtacagcgt ccattattaa aacgctgctc aagaatgtgc ccctagtggt 30360acagctatgg taagtcccac cattcataca ggtgttcttc cttactctgg cgctgatgct 30420gccagggtag gatcatgtta catcggcctg tggcttctca gctgagacag cgtgggctgc 30480aggctgttgc accgattcgt cgtctcaaga aaagcacagc cagcccgaga tgcacgcact 30540ctcccctgga gcctcttaaa gctcaagtct ttaagtccag gagttcaaaa agtccgcgtc 30600ctaaagcttc gcttgtggct cacccatacg gttcgcgttg aagaattgtg tccccacgcc 30660gagctgttgc cgcgccttcg cgctgccatg gcggcagctc cggcactcac catactgata 30720tctggagcga tactttctgg gaccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 30840nnnnnatggc cactgtgctc taaagcagcc ctaagtttag cagcttactg aaggtgctat 30900tttggataga aagttgaaac tctgggggct aattccattc tcacactcca cccccaggtc 30960tactaaatgc ttatcagctt aaagagagta ctagatagga tacaaagtgt ggctcaccct 31020ctctgtgggg cagtatcatg gtctaacagt attattaccg tatggtccat tgaactgggt 31080cgaattagtc cctcatttcc acacttctct tcattagagc ttgagcatga cctcatttat 31140agatggaatc tttgtggtgt taccagttaa gatgagccta tattcagtta aggtggtgtt 31200gaattcagtg tggctgatgt ccctggaaga aggggcaatg gcatgcaggg ggaaagttta 31260agcttctggg aggtgagtgt cagcagcaac acaactgcac atctcatttc caggatgggt 31320tccttctgct tccctgcctt aggggaatgc ctggtttgtt ttatgtcagt gatgatgaaa 31380aacatgatgg gacaaagatg tcatgacttt agaagaaggg catggaacat tagaggctga 31440cttgtcagag cctggagacc agccttttca cggttctaga atgaaacagc tcctgtggtc 31500agagccagag ttttgtcttt cactttgnga ctatttctct gntctccctg attttccata 31560naccanngct ctccatcttc tcctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 31680nnnnncttgg aaagcatccc ttcgggcggc cttttctttg tttcccgaac ccctggcccg 31740agtttacccg ggaataacct tgtggcccgc gtctttttct ttctcccttt caaggaaaaa 31800ccgtggggcc ggctttttct ttaattaagc tctacacggc cttgggaagg ggttattctc 31860ttaaagtttc ggggaggtaa ggggtatcgt ntcccccttt ccacaaactt tggtgggctt 31920gtgggtagac aacacaaaac ctcctccctg tttttaaaac ccccccaaac ccgcgtttgg 31980ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatggccac tgtgctctaa 32100agtctccctg catttctata ataaagctct taaaccatag agagtctctg ctcatcaagg 32160ctgcatgcac tctcgtaggt attgggaaca tcttccctca tccctctctc ctataaccct 32220ggtggcttta gcaaagtagc tccagggtcc ccaggtaggg ctgcccctgg ccgtcccctg 32280aagagagggt cagaggcttg gatgcccatc cagggatgag tggaaaggta gcagccctcc 32340cacacctgac aaaccagaga ataggtaaaa ctctggtggg gcatgggtct atctcccccc 32400tcttcatccc cagggccccc ttttttagct cccacagaat actgacttcc aggcagctag 32460gatgaaggtc ttaaagccca tgcccacagt gatacatcta ttccaacaag gccacaccaa 32520gatgagccta tattcagtta aggtggtgtt gaattcagtg tggctgatgt ccctggaaga 32580aggggcaatg gcatgcaggg ggaaagttta agctttggag caatactgat tagaattaga 32640atcctagctg tgctacttat agcctgagtg gtccagggca agagatgttc atctcagccg 32700ggcgtggtgg cacacgcctt taaatccagc actcgggaag cagaagctag cggatttctg 32760agtttgagcc agcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 32820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttctt 32880ctcctttgta tancatcatt tngacctcga tacgaattat ggccactgtg ctctaaagtc 32940tgcataacaa ggggacataa ggaactcctt ggtcacctac ccccaattct caaaactctg 33000tgacagtgat aaaggaatgg tctgtgtatg atgtagtaat ctcattttca ctagaaatgc 33060gtcacatggt agtctcctgc tgtcactcac agactcacag actcatgttt gaagaatggg 33120aagcttccac ttggcactgg agcggaaggc ctccatctgc tttacctctt ctggacgctc 33180atccacaaac tcagccacct gcaggagtag gggcactaca gtgaggaaac ccacaggacc 33240cagccaagga tcacttgtct caagaaccca gagccatagc ttacaggcag agaactgcct 33300gcttctcctt gtgatcgcat gactgacttc tactgcccat catgagacta tcataccaaa 33360cagtgctagc cgaagatcaa aaattcaaaa ccattttctg ctgaaaatgt atcatgtcca 33420caccactgca cagtccaaac attaagttgg gagcaggtgt ggtggcgcac gcctttaatc 33480ccagcacttg tgaagcagaa gcaagtggca atctgaattc aatgacagcc tggtctacag 33540agtgagtttc aagatagcca gcgctacaca gagnnnnnnn nnnnnnnnnn nnnnnnnnnn 33600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 33660nnnnnnnnnn nnncagcgca ctgggggtca gagcccccaa tcggaccatc agcccttcac 33720cagacctgtc ccaagccctt agtgtcgtcc tttgacagtg gtgtcggtgg tgctggcctc 33780ggttctgctg ctttgagtgg tgaacgtgct ttgcgaattt tgtgggagtg atgggattta 33840tgtggaagag ctgggctttg tgggtggtgt tcttaccttc tgggtagtca ggctgacacc 33900atgtgtacta tcctgaagta gtttggactc ttctgaatgg aaggcgggaa gaagaaagat 33960ctgggttggc atctgatgga gagatggtct ggttatttcc caagctttgg tgtatcaatg 34020ttattcattt agtaaatgtt tggtgtctct ggtgccagtc ccactaaagg acagagtcct 34080tgcactgccc tgtgcaggtg atctgtgctg agacagaagt tgtgaaatgg gctaccttcg 34140ctggtgctga agctaccaac acttccggga ttccaggcag caccttcctt gccaattcaa 34200gagtcacttt ggaaggtgcc aagatgaagg ggtgaatggt agtgagagaa atgcctatga 34260tacgcactat cttcactctc tgtcacacca caccttctac acaccccnnn nnnnnnnnnn 34320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34380nnnnnnnnnn nnnnnnnnnn nnnnnnnatt atttatacgg atggcaagct catgtgctct 34440aaagctncca agacagcaat tgcacctttc agaacagcat caatgtccaa acatgagcca 34500ggtcctccca aatggcaggc gcatcagtct ttagagcaca gtggccatnn nnnnnnnnnn 34560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 34620nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa atttaagatt acgcatggcc agctccatgt 34680gctctaaagt tgaaacctaa tgtatagcac atggtaatgg tgtaggtatt cttgcctagg 34740tgagaggctc cattctgatt ctagagttcc tgtccccttc aggtccctgc agtttccctg 34800tctcaggatt ccaagcacac gcctgtaatc ctgttttatg ccgtgtttga gatcaatctc 34860tggactatgt atattttagg taagtacagc ttacaaccct tctttaacga cccatgtgct 34920gggacagagt gggaagcttt ttccatgact gggactacag actcagaagg tggccccagt 34980cttcctgagc aatgacgtgg ctgcttttta gtttctattg gctatatggg ctcttctctg 35040accctaatga caaggtacaa tagcttgtag gtggtgactc agtttcaggt tgttatgtta 35100acttcgtgtt ggttcagatg ggggagtgag gcccgtggct tttaaccaga tattaagaga 35160taactaacat tctaactttg tcttcaaact ggtactggtt tattaatttg ggtggctttc 35220tttgtttcta gatttaaatt aagttggctc tctgtgtgtg tggtcatctg cttaaggtat 35280agaatgaaac ttggacttgg tgtatactta acaagggcct ttgannnnnn nnnnnnnnnn 35340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 35400nnnnnnnnnn nnnnnnnnnn nnnngtgtgt agtacgtgat gggcaagctc catgtgctct 35460aagttccacc gcctttgcaa gctagatctc cctttcttct cttaatggcg actcgcaagc 35520tcgaaagcag ccacctcagt ggtgagccaa ctgttggccg gttaggcaag cttatcttcc 35580ttcttccaga gttccagcgt ggcactaatt atgtccctgt ctgctcagag ttcacctgcc 35640tcagctgatt acctgctgct cacaagaagc acaatgagaa ccctgtatgg agctgatctg 35700agtggctagt tgcaagtgtt ccctccgatt ggcacacatg tgtactgaat ttcctcacaa 35760cccatctgct ttcgccttct ggaggaggct gcattccact atcccaccct accccaagaa 35820gactggaaga aacaagatca agccatggct ggcgaccttg ggaaacatga cctggggagc 35880tagggacagc catcaaatgg aatggcttag atgccagtca ttaaggaata cagaaggaca 35940tcaactctct tggtggccgc ttccctggaa tcagctaatt tatctaatta gagttagagg 36000gaaactctgg ttctctcttc cacggaaaga attcttaata acagagcctt accaaagact 36060tgcttggaga atactttaag aattactttc cacaccccgn nnnnnnnnnn nnnnnnnnnn 36120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36180nnnnnnnnnn nnnnnnnnnt caaagtctgt ggtgctgagg agacttctgt ggcaaaggcc 36240acatgatcct gggtggaaag tggtatgggg gctccaagct ttgaggtggg aggtatatca 36300gaaacctcag gtaccccagt ctgtcatcta gaacacccag tgttcagcca aagcaacagg 36360acccccaaac aacaaaagca ctcccagtta ctggaccaca agatgcagac cacaagaaaa 36420tgataatagg caggtagatg atgccagatt taccagaaga aaacataaag atgatcacag 36480acacagaggg gctcaccagt gaagcaacaa cggtaaaggc aggaatcata atgtcacttc 36540agataaagaa tatcaacagg agacattgtt ttaaaaaatt attgggctgg gaaataagta 36600aatcattaga aagtctccgc aacagagttg aactgcctga aagaagaaac acaaactcaa 36660ggatagatca gtaagattgc tttagagcac agtggccatn nnnnnnnnnn nnnnnnnnnn 36720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 36780nnnnnnnnnn nnnnnnnnnt catcccagtt ccagaaagac aaatatggta tgtatattct 36840cacatatgga tattagcagc accctactac ttggttccag tgataaccaa gctacaatcc 36900ataaaaccac aaagattagg catagagtaa aaaacctgag agaccagaca gccctcctta 36960ggaagaggaa actgaataga taattatgga tgggtcccag tgggtgactg gaacaggagg 37020atcaagttgt ggaggagaca aggggggaat atggggagaa acaactcaaa ttaagaaaca 37080tttgaaggga tttctggaag cctaatacag tagaagcttg gtgtgacctt tgctgtacat 37140aaccagtgtc tctctcccag gacttctcct taattttgta gccactcttc ccactgccac 37200taatacctca agccagtcag cctgtggttc tgcctgtggc ctacttgagc tgtgtggacc 37260gagaacgtac cctctggctc accccctcct cttgggatgt ttctttctgc tttagcgctc 37320acacatgcct taaaccacta cttattttgc tcctttagag cgcagtggcc attttcgtgn 37380cgtggtcatg atgctaaagt agcccgccgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 37500nnnnnnnnnn natggcccgt gtgctctaaa gccaaaacaa aacaaaacaa actatttgat 37560ttactgtaat ttggctctcc tagtctctga taaatcatgt ttgttacaaa ccatgtttgt 37620tttgttttgt ttttaaggtt tatttatttt tattatgtct aaagtgctct gcctgtatgt 37680attcctgcag gccagaagag ggcaccagat ctcattatag atggctatgt tggaaattga 37740actcaggacc tccatagcca tctctccagg cccagaccag tgtttttgag tgaagcttaa 37800ccggcctctg tgtactcaac tttgtgaatt aagaacaact tttttctttc ttcttctctt 37860ttcttttctt ttcttttctt ttcttttctt ttcttttctt ttcttttctt ttcttttctt 37920ttcttttctt ttcttttcct ttcctttcct tttccttttc tttccttcct tccttccttc 37980cttccctgga aaaaaaggtc cgatggtaaa tggaccttta aagcgattgg agcttttttg 38040aagaaacaca cataactgtt gccctgctga taaaaacctt tgatccttgg ggctttaacc 38100tctgccttag attcgcccgg ggcttgtgca aaaaacatag aaatttccac ggggcttttt 38160tctacctttt tttttccaat cacagctttt ctccaaagtt tttactctct agccaacttt 38220gagagacccg ataattggct tcttgcgcgc cctttcaagg gtgctgcgaa tttattattt 38280aggttcttgt gccgacttta aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38400nnnatggcca ttgtgctcta aagccctccc cagccctggc tccccattcc tgctagcact 38460accccttgtt gggtacatat tcccagaagg ggctggcacc attgaaggcc tgcgcctttc 38520tgtgaccctt tgccagtctg agaagctttc tactgactac tgagtggaga ggaaagaggt 38580ttatttgttc tcatcttttc acagaatctt tgatatacga tagaaaggtt cacaaaaatg 38640agccagttaa attactgtgt cagtcgctcc tgaacactga ctggtgcctt ctcacactgc 38700tgcgtcaatg gccacctacc agaacctcta tctccaacct ctctcaccca agctgctgaa 38760gctagtagct tactgatcac caggggttta gttcagggtc tgatacannn nnnnnnnnnn 38820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 38880nnnnnnnnnn nnnnnnnnnn nnnnnnntct tcctcttctt ttacggtcgg gccaagctcc 38940atgtgctcta gagcacagtg gccatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39060nnnnncgata cgaatatggc cactgtgctc taaagagctc cttcagtggc tcctccccag 39120cactaagccc ccaaaagacc caaatacagc tgatgcccat cctcaagtag attcaggaag 39180ctggcagact gctacaaagg cgttgcccct acgccaatct tctcccaccc acctcattag 39240tccgatgccc acaattctca cagttggtgg ccatgatgat aacctcttta aagtggggga 39300tttctggaag atggagttaa agaagtctat ctgcttcaag gaaaacaccc ccacccccac 39360ccccaaggaa aagcaaagcc aagtcccaca caaacagatg caggacccaa gagtacaata 39420aacgggcctg tgctcacaaa cacggagacc tggccacact cagaagccag gtatgcagta 39480tgtagcttca ttaaaggttc atcctcacca ctgaggataa agcactggct ttccctgaag 39540ggaaggctcc cgtctgtaat ataacgttca tgcccgagtc agctgaagag caacccaatt 39600gcctgctgaa agatacggac aagcttatgc tggcctccgg gtgaattcaa caaatattaa 39660atgtcttgta tgggcccgac tttgggctgg ttgctggaga ttgtggcctc tatctttaat 39720aaactctaag tccacaggcg aaggaaaaca tgaagttcgc tgtactacag agacctctag 39780cacagttggg aatataacgc atcatggagt gagcccctga tgccactgtg tcagcagcaa 39840gggacaggga gaatgtccag gctttagagc attggagctt gccaatccgt actcagagna 39900annganaccc cctacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 39960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatggc 40020cactgtgctc taaagtcctt tctcttcaac ctccagtcgg tccccctgcc tccacccaca 40080cgctgttccc gtcactttcc tcagaacttc cctctgccct tcaccgtgac cagttcagcc 40140tcctcccttg tctccacaca aaacttctgc catcctcgat ccacttacct ctcaggcaca 40200gggggatgtc tgcctataat cacggcactg gggaggctga ggcaggagaa ttgtaagttt 40260gaggccagtc tgggctgctg tgggaggtcc tatctcaaac aacaaaacta agaagagaaa 40320aagaagctgc caagcttggg ccaactggtg gaggagatgg cccaagtctg agaagacata 40380tgggatgaag gcgggggttg gggtgagggc gtctccnnnn nnnnnnnnnn nnnnnnnnnn 40440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 40500nnnnnnnnnn nnnnnngacc atgattacga attatggcca ctgtgctcta aagctctgga 40560aagagctctc ctgagtcaga agttattaaa aaaaactaac aaacaaataa agcactgatc 40620agaaagcatt

ccttcaaagg gaccaacatt tgctatattg tttgtaaaac aactcgcatc 40680cacgaacaac acagtgggca atcagctaag aattaatgga gttgaatggc tgtgtttggc 40740taagaaaaga ggtgtagaga cttcagttca aactagggag atcccagaat ttgccaggct 40800gttttgccca gaagagagga cccaaatcct aagaatatgg atggagtcaa tccaagtgtt 40860gaaaaggaac aaagcagaca gaagcttcaa gcagtggtga gctagtgaaa tgcccatgga 40920gtcctacttg ttttagtaac atacaaggca gattaatcag ggctggggaa aggatgtcac 40980ggtccagaca ctgagttcac caaggatgct tgcagacacc tgttctctct gcacttcagc 41040ctctgaatgg aaacccagca ccggtattat ctcccctcac atcggacgcc tacactttca 41100gtattttcag gcaatagaca ggtttccgtc acccagggat gttcttcgag ctgtctttct 41160gcatgaaacc atttctttct cattatagca agaatatccc aggttcaagg tgtggcaccg 41220ttttccggac tctcaggcag tgaagtcggc cccagctctt tagagcacat ggagctggcc 41280agtccgtacc acagagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 41340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgat 41400gaatccccta atgattttgg taaaaatcat taagttaagg tggatacaca tcttgtcata 41460tgatcaaatg gtttcgcgaa aaatcaataa tcagacaaca agatgtgcga actcgatatt 41520ttacacgact ctctttacca attctgcccc gaattacact taaaacgact caacagctta 41580acgttggctt gccacgcatt acttgactgt aaaactctca ctcttaccga acttggccgt 41640aacctgccaa ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg attgttaggt 41700aatcgtcacc tccacaaaga gcgactcgct gtataccgtt ggcatgctag ctttatctgt 41760tcgggcaata cgatgcccat tgtacttgtt gactggtctg atattcgtga gcaaaaacga 41820cttatggtat tgcgagcttc agtcgcacta cacggtcgtt ctgttactct ttatgagaaa 41880gcgttcccgc tttcagagca atggtcaaag aaagctcatg accaatttct agccgacctt 41940gcgagcattc taccgagtaa caccacaccg ctcattgtca gtgatgctgg ctntaaagtg 42000ccatggtata aatccggtga gaagcttggt tggtactggg taagtcgagt aagaagaaaa 42060gtacaatatg cagaccttag agcgcaaact ggaaacctat cacacnnnnn nnnnnnnnnn 42120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 42180nnnnnnnnnn nnnnnnnnnn nnnnncttgt tacgcgctgc tgggctccat gtgctctaaa 42240gctttagagc acagcggcca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 42300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 42360ntttcgtcta cacctataag atctctttcc attgaggagt tcctcgggat catgatctcc 42420cgcctcccta agcagacggc gcgaaggatc gaccatagct gcgtggtaaa caccgacgcg 42480tactcttcat atatacgcac ggcgcgataa cggaggaacg ggtgtgctct tgcgggaatg 42540ttcagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt 42600ccgttggcgg attccagcgc ctgacaaaaa atgttgtaca actcgctttc gcccggtgcg 42660ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgctttc cgcaccattg 42720ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata 42780ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc 42840tgtacgcggg cttcttgctg ataataacga gctacggctt cccctggctc aaccacaatc 42900gccccggcgt cgccgtcgat ataaatcgtt tgttgctgcc acggagtaag ggcatcaata 42960tccacaccaa ccagcgttgg aatgttgaac gaacgggcaa ggatcaccgc atgtgaggtg 43020gtgccgccgc ttttgagcaa caatcctttg aggtgatttt tatccagttc gaggaactgg 43080ctgggggtca gttcatcagc catacaaatg gcgggctgcg tcagtttgcc cggtgccggg 43140aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg 43200gcacgttctt gcaagtagct gctgctggaa cgggaaaact cttcgcaaaa gtgattcgcg 43260ctggcaacaa ttgcttcggc gcagcttaat ccggcgctga cgcctgccag taaatgttcg 43320cgcagggaag tatcgccagc ctaggatcgg ggagctttca gaatcgcgct ggtagcaccg 43380tcgctatcca gcaaacgaaa ctcaatgttt tttagtacca gcgtcaggcc gttttccagt 43440gcggattgct ctgcgtcaac gcctttggct gcgggaagat tacccagcgc attgagatct 43500aaaaaaaaga tctgcgtcgg aatgccgcct gcactatcgg tgcacaccgc gcgggcgctg 43560gataattgcg gattcagatt ggtcagtgat acccggagat gtttcagttc gccagattta 43620acttctgtca gcggcgcatc gcagtgtgtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 43680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 43740nnnnnnnnnn aaggacaatt ctataaattc ctctgatcgc gcatggcgca actccaatgt 43800gctctaaagc acagtggcca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 43860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 43920ntagccccct tcctaaatgc tcatagcact gacaggcaac ctctaggctc aggatccctc 43980ttgcctggca tgcacctgta tgatcttcat gtctctctta tgtcaaatgg ctaattctct 44040tttgtatcat acctgctggt ctctcgggca tccttacttt agagcacagt ggccatnnnn 44100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 44160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncata caaacaatca tgacctgcat 44220tacaattatg gccactgtgc tctaaagaag agcaaacagt aagagtccca caatgtgctc 44280tggcctaatg gcacagaggg taacaaaccc cacttatgag acaattcttt tgtcacctat 44340gtataagaaa agagcaagca ccaggaatat tctatagtta aatagatctc aagaagtaag 44400attgaatcac agtgacctag actatagact gagaccgtct caaaggttta aaaaaaagaa 44460aggaaggaaa ggaagacaca ggggatggag gagaagatgg gagagccttg tttatggaaa 44520gtcaggagaa cagagaaata gtcacaaagt gaaagacaaa actctggctc tgaccacagg 44580agctgtttca ttctagaacc gtggaaaggc tggtctccag gctctgacaa gtcagcctct 44640aatgttccat gcccttcttc taaagtcctg acatctttgt cccatcatgt ttttcatcat 44700cactgacata aaacaaacca ggcattcccc taaggcaggg aagcagaagg aacccatcct 44760ggaaatgaga tgtgcagttg tgttgctgct gacactcacc tcccagaagc ttcccactct 44820gtcccagcac atgggtcgtt aaagaagggt tgtaagctgt acttacctaa aatatacata 44880gtccagagat tgatctcaaa cacggcataa aacaggatta caggcgtgtg ctctttagag 44940cacagtggcc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 45000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatggccac 45060tgtgctctaa agagagagag gggaaacaat aagtagacat acttgtgagg ggggacctat 45120tttcaataaa gacagaaaag gggaaacacc ttccttaaca atgagtcaca tctgtccttt 45180aactccatct tcctctatgc tgggatcttg ctgggaggag tgacatcttc atccttgcca 45240cactgtatct gtatttctgt ctgtcactca tccaattgaa gtttatctca tttttttttt 45300tttttgggcg ggtggcctga actcatatgt agaccatgtt gtccacaaat cacagagtaa 45360gtgacacctc tctgcctctg cctctgtctc tgccccctct gcccctctgc ccctctgccc 45420ctctgcccct ctgcctctcc tctgcctctc tgcctctctc tctgcctcta tggctcatct 45480ctatgcctct ctgcctctct ctctgcctct ctgcctctct ctctgtctgt ttggctctga 45540gacagggttt ctgtatgtat ctctgacttt ctggaactca ctttgtagac tatgctgctc 45600ttgaactctc aaagatccac caatctccac tttgtgtgtg ctgggattaa aggcatgtgc 45660catcacacct ggcataaatt aatgttttta aactcactta tcattgcttn gctaagaaag 45720aatgagcctc gggaaccttc aaccttagtg ggagccattc tctgtcagtg ggtccctcac 45780aggatcacta gtattctacc tgtctctatg cctttataca agcttatttg gtcacctttt 45840aaaaaattgt tgccacccat caaaactaac ttgggccaat ctggggctat tttaatggtn 45900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 45960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgccaaagct tgcatgcctg 46020cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag 46080cggggcacat ttcattacct ctttctccgc acccgacata gannnnnnnn nnnnnnnnnn 46140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 46200nnnnnnnnnn nnnnnnnnnn nntttaggtc gcgactgcca gctccatgtg ctctaaaggc 46260tagatctctt agttaaagga cttttcacag gttattggat attttcaaca gaacatgcta 46320tggcattctc tctctctctc tctctctctt tctctctctg tgtgtgtgtg tgtgtctgtc 46380tgtctgtctc tgtgtgtgta tctgtgtgtg tttgtgtgtg tgtgtgtgag tgtgtgtgtg 46440tgtgtgtgtg tgtgtgtttg gttggttttt caagacagtt tctctgtgta gccctggcta 46500ccctggaact cactctctct gtagaccatg ctggccttaa actcacagag acccacctgc 46560ctctgcctcc caagtactag gattaaaggc gtacaccacc ccacccagct agtatacact 46620cttccctgct gagccatttc ttgtcccaag gctgacattt ataatctgtt aactgtcatg 46680atgtaaagtt gggttaaagg tatcaatgac gctggctcag gtaaacctag taccttactc 46740taaggtacta agaatatact tttaaaactt gctataatgg ctatggaacc accaaattgt 46800ttttcagtct tgaggatagc ctgatctcta atattataga gtctattaca ggtgagggct 46860ttttgagagg ggtgtgtgtg tcgggggggg gggttaccct ctattctagc cacttctcta 46920ttgatcattt ttcttggtgg aaacttcata ttacctataa aaattaatat tttagttcta 46980agaagagcag atgcagatat aaagatttat gtgccatggt aagtgactca agtatgcttt 47040tttgtgttcg attataggac tttaaaaaat aagagaagct aggatggaca gagctctaca 47100tgaggccaat cttccacagt gtagtgtctg ctccagtcta aggattggtt gctaagactc 47160tgctttttga cacactcttc aaacacagga aatgcttctg atgcttatct tcatgtgtgt 47220ttgctaatgt gcatgtaatc ttatgcaggg ggagttgaaa aggaacgtgt taagtgttat 47280aattctggaa aagtaccatc aagccctgag aacccttttc caatgtgtac tttctgtttg 47340tgtgtggttt tgtgtggagg agggcagggg tccaagacag ggtttctctg tgtagccatg 47400gctatactgg aactggatct ttatgccaga ctggccttga actcagagat ccaaatccct 47460ctgcctccca ccgagggagt gtgccaccac tgccaggcac atatgtactc gttgtgtgca 47520ccctcatcac agcaggctca ggcttaaagt ggtttaggct caggtgctag tgtgcttcat 47580tgatgtgcac aggagtgtgc cttattcccc ttcagctctg ttcaggcttt gttggttcat 47640ttgttttaga tgtacttaca tatgtaaata aatgttttgc ctgcaggtct acccgtgcac 47700cacatgcaca cagatacctg atacctgtgg aggtcagagg aagtcctcag acctagagtt 47760ggattaacaa tggctctgag ccatgtgcac tgctgagcta tctctcaagc ccctctgttt 47820actttttaac tgttaactga tgcatactcg cttgcttcct taagctgcct gaggggctct 47880gccagcatct tgggtgggta ctgactctac tcttgttcac cacagaccac ttggtagccc 47940atggaagaat ggcagagaag gaagctcgac ggaagttcaa acagatcgtc acagcggtgt 48000atttttgtca ctgtcggaat atcgttcatc gtgatttaaa agccgaaaac ttacttctgg 48060acgccaatct gaatatcaaa atagcaggtg aggtgtgtgt gcacgtgcgc atgtgtgtgt 48120gtgtatgtgc tgaagacagg agtcatggtt aaagtaggga cagtttaggc ttttttaaaa 48180atgagagctg gggcttgttc atgctggagg cacttttctc actgagctca cccccactcc 48240agacttcact ttaataacct gaaaattaat aaacttccaa aggacatttt aaaatatagt 48300attagctaga actctttaag aagaaagaaa aaggtagtat ttcaaattct attactttga 48360taaatccttt gtcaataatg tagagtagac tatttttttt atagcaagaa agaaaactgc 48420tgaaatgatg tgctcagctt cttctgccta agctgtttgc actgccccag ccncagacac 48480agccacagcc ccaacacccc accgcgcaca tccnnnnnnn nnnnnnnnnn nnnnnnnnnn 48540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 48600nnnnnnnnnn nnntctcgat caacagctat gatcctgatt acgaattatg gccactgtgc 48660tctaaagcca cataaagacc caacaaaaaa agagaattac agtgatctcc cttatgaaca 48720tagatgcaaa ttttctcaat aatatactta caaactgaat ccaaggacac agcaaaaaca 48780tcatccacca tcatcaagta gtcttcatcc caaagattca gggatggttt gacatatgta 48840aatcgataaa tgtaatccgc catataaaca gactgaaaga aaacacacac acacacacac 48900aatggttatc tcattagaca tagaaaggcc ttttaaaaga atccaacacc ccttcatgat 48960aaaagtcctg gaaaaattag ggatacaagg agcatacata aacacaataa agaaagttta 49020cagcaaacct atagccagca tcaacttaaa tggagaggaa acacaaagca actcccctaa 49080aatcaggtac aagccaaagc tatccactct cttcatacct actcaataca tgacttgaag 49140tcttagctag atcaataaga cacctggaag aaaccaaggg ataccaatta gaaaggaaga 49200agtcaaacta cctttatttg caaatagtat atttaagtga ccctaaaatt tccaccagga 49260accaacctcc tacagctgat aaacactttc agcaaagtag tggatacaaa attaactcaa 49320aaaaaaaaaa aagaaagaca gtaagatgct tcctttatcc tactgcagac attcgctcaa 49380ctgtgttcat tgctgctctg ctcctaatag tcagaaactg ggaacagcct agacgtccct 49440caacagatga atggaatata tatatattta cacaatggaa tattacttag ccattaaata 49500attaaatcat gaaaatcaca ggtaaatgga tggagctaga ataaaatcat cctgagtgag 49560acatcccagg tccagaaaga caaatatggt atgtatattc tcacatatgg atattagcag 49620caccctacta cttggttaca gtgataacca agctacaatc cataaaacca caaagattag 49680gcatagagta aaaaacctga gagaccagac agccctcctt aggaagagga aactgaatag 49740ataattatgg atgggtccca gtgggtgact ggaacaggag gatcaagttg tggaggagac 49800aaggggggaa tatggggaga aacaactcaa attaagaaac atttgaaggg atttctggaa 49860gcctaataca gtagaagctt gctaagatag atccatatat gaaggcagtc caaatggaat 49920caccaaatat tggaagatac aaggccctaa ctaaacatct ctgtcaccaa atgaagcttg 49980tacatttaat tgagttattg tacaaaggag accctatgcg aacctttcaa caacccaggc 50040tgctgctaag actacaggtg gatcttcaca aactgacagc gaggccccat tgctgaggct 50100aatacccaca caactcgtag aacatgaaca agtcaagccg gtgcctacac ggagccttca 50160cacctacatt ccagtccttc tttggtgcag gaaggtacgg tgcatgctac caaagagaaa 50220tataaacacc aagccagcca tagcctcaca tgagagtcag agcctctcct ttcttctttt 50280taatctcctc ttatttttat tttattttat tttattttat ttacatcttt ctctccttct 50340catgtacatt atggcttcca gtctagtgtt tttatgggat tcatgagtgt gccaccaaag 50400ggtctctgtg tctgtatcta tttcttgtgc cttgggttct tttctgtctg cttgttttgt 50460cttattttga tgtgttaatt tttgttttct tattttattt tattttattt tattttattt 50520tattttattt tatcttatta gtatccctta gaagattgtt tactaatgag agacagaaag 50580ggggtagatc tggatgggag gggaggtggg agagcactnc aatgggaacc ttcanntacc 50640taccttcgat ccagaactat tttcaataca aactcactac ggaaacacct ccgttcaaag 50700tatcacatcg tcctttactc ctctttcttc tactggactt gctggacgaa gactcctctt 50760ccttgccact gatccagttc tgacgccccc tccactaaan nnnnnnnnnn nnnnnnnnnn 50820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 50880nnnnnnnnnn nnnnnnnnnc ctccccccct ttttgtatca tcgtaccagc cagctccatg 50940tgctctaaag cacagtggcc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 51060nntgagcttc aggaaccagt gccccaatta ctgtaaggtg gtggcaagcc aataaataaa 51120tgtgtgccac acagatgaac tgtgctgcaa ggaggaggtt atttgaaatg ccaggaacac 51180atgacacagc tttaatcaca acaaccattt cagacaagta gtaaggggct gggacttcag 51240gctcagagag atgaacatct ctctcttttt tttttttttt tttttgggtt ttttgagaca 51300gggtttctct gtgtagccct ggctgtcctg gaactcactt tgtagaccag gctggcctca 51360aactcagaaa tccgcctgcc tctgcctccc gagtgctggg attaaaggcg tgtgccacca 51420cgcccggctg agatgaacat ctcttgccct ggaccactcc agctataagt agcacagcta 51480ggattctaat tctaatcagt attgctccaa agcttcctgg atgccagcaa aacccatatg 51540cattgaagac aaagtgctaa tttaagagga agctgctgaa gtgtgaatga cttactatga 51600atgctctcac ttacttaaga taacatttcc cagttgttag ttcagtttta aaagggataa 51660tttttatctg tcccagccaa cttctaaata aatgagacag agactataag atttatttaa 51720ccaccgccgg gtggtgtctc gaaaaaccaa aaaccaaaaa caaaaaaaaa aaagagagag 51780agagagattt acttaaccca gctttacttt atggtacagt atctgagcat ttattagtct 51840gttttaatcc tgcgagctaa cctggctacc tctcaggtgt attctcatgg tgtctctgtg 51900accctttcct tatagacacc cgacccctgc tgggattgga agtccagcct tattctctcc 51960cccaatcagt cattggatga tcagctttta ttgacaaatc agaaaatgaa tggggagcaa 52020tgtttacata gcattgaaat aagagattat tagaataagc attgcagtgc catgtctgga 52080ttgcaaccag atatgggggc agaaaatcag cattggaata acaaaggata atctttacac 52140agtgcacaat aacattatgc ccacatccaa tggtgccaac aggcatgaaa cccaggaatt 52200cccacaaacc aatttattta tgccatggag cattcaagga ctgccagtag ggcaggcagt 52260ggtgagggca aaattgtgta accattgaat gctacaccgt gaggtccatg ttccattgag 52320aacttttatc ttgttaaatt ctagatcttc acggacatta acatagcaca tactgtgtgc 52380cagcttgctg tcacctttaa aacatatgtt attttaacca catacattct ttgtatcctt 52440ctaaacaaaa ctagtattct cccatcaaac tggttcatta ttattattat tattattatt 52500attattatta attattgtta ttattagact atgaagacta gtactctgct caaactttac 52560attggaggca gataaccaca ccactgtgct tatctgatca ctgatcaaac aaataggagc 52620cattttcttc ccagttaatt cctcatttgt tagtctgtag ttttgctttc ttaatatcat 52680acatagctag tttatgtttg taatctgtac aggatgcctg cagaagccac tgtgcatttc 52740cctcagagcg ccattggctc tcttctcttc tcttctcttc tcttctcttc tcttancnnc 52800ccnncncnnn ncccccctcc ccttcnctcc ccctttctca tttttttttt cctattaact 52860tattatcatt tccttcccta catcaaacat ttaaacacct ttcctgattt tcccttttaa 52920catatcaaaa actttctcta attcaacagt taaccccaaa cacctcattt cacccctctt 52980tttaaacaca cccactttcc ccataattca naggggcact taacaatttt ttccccaaaa 53040tgacccaaat ggccaccatt tcccattcct ccgctattgg gtggtcgctc tcaaaaacgg 53100aggcctgttt gggccccaag cttcttaaaa acatggcact tctaaatgag atcctctctc 53160cgcgacaata acctcagcgg gattggttaa tagcacccca cctcttcccg ttaacgcccg 53220tggagtaaga ggggaccaca tatggaccca ccctcttttg gggggatgat ttaggcgctt 53280gagcgcccca taaaccccga atatatgtgc ctctcttttg tccgcgggtt caacttcata 53340agccagcgcc ttttaaggac tcggagtcgg tcatgcgctc tcttttatta tagagacnnn 53400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 53460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata taatagaata aaataataat 53520aagtttttaa taagcggttt ggtcaagctc caatgtgctc taaagcacag tggccatnnn 53580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 53640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga ctatcagcta tgacctgatt 53700acgaattatg gccactgtgc tctaaagctc tgcctagcta acttgtgcat tcctttctta 53760ctggtggtta cttgttgctg tgacctgtca gcatttgtct gacagatgca agtgcatctc 53820ctgctgatta gcagacagag agtgtatagc aactataaat agaaaatagg tggttttttt 53880tgttctttga cagtttctat taatacattg tatgtccaga ggaaaataaa gtagtttgtg 53940ctgaaactga agtattgttt gaatgtaacc gccttgcaca gccaaggact cataagctgt 54000cctcagattt ccctgtaagc agggcaggag ttgagagttc agatttatat cttgttccag 54060gtcaaagcac ctgagcaagg ccctgccagc tgtggtgctt ctgtctctta gaggcagacc 54120tatcctttta agtcctagga atagtgtgtt tccatcccgg tctcagactg acggctgtgc 54180ttgtgttctg aagctcacat agtcttccac tgtgttgggc cttggcatga cacagcaggc 54240aagagtcaaa ggctttcttt atggctggag actaagccag gcactgtgac tcgtcctgcc 54300ttcgcctgtc aaaggacatt actgtgcagg aaaccatggt tttcctttgt cttcatgtca 54360gttgtggcta ttcccataga attaggtaat gtggaaagac ctggcaatgt caaaccttct 54420tttgtaatta cctttttttt ttgtagcttc aatgaaatga actgtaaaca gcatatactg 54480aagctctcag aactcagaca tgtgacatgg gatttgaagg cagcctggat gcattgcaag 54540accctgtctt ataaaaaaaa aaagtgcatt gcaacaggcc tcttagctcc ttactgttgg 54600tgaaacaaat tagcacaaac tccaaggctg caagcacggt ggtgttctaa gtcagctctg 54660gaggtcagta gaataaaaaa catccggtta gcagctgtgg ttcctggagg acctaggggg 54720aaagcccaga ttcttgcctt ttttaccttg tagagcgaac ctgcctttct tggcgcaggc 54780cccgtcttca cagcacttcc caaacttcat ggttccattg tggtgactgt tctcctgatt 54840cctggtacta aaaggagccc tgtcattaca tcaggtcctc ctagataatc caagactacc 54900tccacagttt cctgatattg acttggtcac ttccatagag agctcctttg acattcacga 54960ggataggatg cttgccacat gggtgtgttg gaggacacaa gccattccca tgccatcccc 55020agagcccttc ccatgccatc ggattattaa gtagttgaaa gaaagccctt ctttatattt 55080tataattgta ttctgcatat gtgacctaag tttaaagtgg tttttttttt ttttttttgg 55140ttttgtattg tgttgtgttg ttttatgctt ggttcatgat ttttacgtca gggccattct 55200gattaagtta aggaaaattc ctgtgtcaat tagttttttc tggagaaacc aaaaataaaa 55260gcaaaggtca ttgataattg agactaaaat tactgaaaaa ttagtggaag gtgatttttt 55320tttttttttt tttttttttt ttttagtttt tcgagagagg gtttctctat agccctggct 55380gtcctggaat tcactttgta gaccaggctg gcctcgaact cagaaatcca cctgcctctg 55440cctcccgagc actgggatta aaggcgtgcg ccaccatgca gaaggtgatt taaaaaaaaa 55500aactggaggt ggggactgga gagatggttc agtggttaag agcactgact gttcttccag 55560aggtcccgag tttaattccc agcaacaaca tggtggctca caaccatctg caatgggatc 55620tgatgccttc ttatggtgtg tctgaagaca gctgcaatgt attcatataa ataaaaacat 55680aaataaatct

tttaaaaaat ttttttggta agtcattaaa acaaatccaa gcagttgcag 55740taagaatgta cccactggag tgtgagtgaa gctgctggga cgctggcagc cagccctgtg 55800ccctctgtgt tctaacacat aggctttggc acagttttaa agttcagttt aagtgtttac 55860attagtattt gttttcagag caaaacaaag actaaattgg gactgtagtt catggttgcc 55920tacagtgcta aatgtttatc tttgggtgac tgctcatgcg ctcttttttt ttccatgccc 55980ccattatgtg gtttctcagt cattgctcga gcttggggtg tttgcacaag tattagatgg 56040gattctcatt tacacttgcc ctatcttcct tgaacctaat ggatcactag gagaaatgcc 56100tttactgaag tccaagtaga acccgagtaa cttcagagtg gactgggtga taagtaatga 56160aatcaatttt atttctaaga gtgtaaagca actattagag ccctgttcct gtcagtggtc 56220attttggata ccatattcga agccttagga agaagctact gtatatgaaa gacatatatt 56280tacaagagag aaaatgagct ttttggttgt gtgtgtgtgt gtgtgtgtgt gtgtgtaaga 56340atcatcttat atagctcagg ctggcttcca actcaagagc ctcctacttc agtctctcgc 56400taggttataa tgtggtcata caccaccatc tctgccattc tgggggtggg gaggggaaaa 56460gagtgttaat aaaccaagcc agagatgtta tcagtcctgg aacgcctatc ttagccacta 56520gtgaggctaa agtagcagaa ttttaagttt gaggccagcc tggacaacgt ttggagaccc 56580tatctcaaga aatgaaagtg ctaggcagaa tgcttaccta ctttcaggtc ctgagttcaa 56640tacaaaaaaa aaaaaaaccc caaatcctca gtctttactc cctcatcttt aaattatgtg 56700tgttaaggaa caacttattt tggactaata cagattttaa ttatgaaatg taagcagtta 56760acatttttat gccaggtatt tatacaaggg gcttttgttt taaaagcatt ctggtagctc 56820agctactaca ggtatttgct ggcaagttcg atgacctgtg ttccattccc agcacccatg 56880tactgcgagg aggaaactga tgctgacaag ttagctgtcc tctgatctcn nnnnnnnnnn 56940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 57000nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggagaagagn agagaagaga gaggagagag 57060aaaaagggaa ganaagggag gagaaagaag aggagagaag ggagaagaaa aggaggaaag 57120aagaaaggag gagagaacaa ggagaggaga aggaagagag ggagnaagaa aaaggagaga 57180agggagaaag gggagagaag aganggnagg aagagaagga gggagggaag gagggangga 57240nngnaggnag ggagggaggg agggagggag gaaggaagga aggaaggaag gaaggaagga 57300aggaaggaag gaaggaagaa aacttcagat caaaacaaga gttcagagaa tttcaaatgt 57360gaccaagaaa gctaaggttc tacttagatt agaaggcaag gaaaactgcc ttatgtctca 57420caatgtagga agccagtctt cttcaactct tcgactcaca aattctcttt tgttcttttc 57480cttttccaac atttaaaagc atttagttag ttagttgggg ttgggggggc atgacttggt 57540atgcccaagg acaacttgtg ggagtcactc tctccctccc tccctcatgc aagctccaac 57600tcacactgta gtaccccaga gcttaccatg ggtaccagct accaggctgg ccttgagctt 57660gaagcaatct tattcctctg cctgccaaat gcttcaattt cagatgtggg ctaccatacc 57720caggtacaaa ctccttttaa aacagtattg tcttaaaacg tcccaagaaa gacattaaaa 57780aacaaacaat aataacaccc ccccccccca gttctgttgt ttttgcttga tttacgatca 57840aatggtttag gcctgtagta catggaaatc agtctcaatt ctcctaccca ttcttcaaca 57900aagaaacaaa cagtctagtt aaggggcttg gtaaggtggc acatatgtaa ttccagaaac 57960ccaagcaggg gaatcttgag tgtgaggcta gcctctattt gataaaagaa aaacaaaagt 58020ccttcataaa tttcaagcag agtcatgata ccattaacag cacccacact aacttattta 58080tgaaaaggat tcatttcagt tggatataaa agctgcataa acacggaaat taattaatta 58140caataggtaa atgcttttaa tgactcggca ttttgagtgg acatactaga acctaaacca 58200ccaagtcagt ttatctgaat tatttttgtg ttttgttttt gcaaatgata aaatgcatta 58260gcaagcatgg ggccatgatt tataataaac aaacaaaata aataatatta tactatacca 58320agaatgcact acagtatgta tacatttata gatatatggc tactagtata taatgtctga 58380attgattatg tctgtctaca tcatgtgtgt atgttcatct gtctatactc aagtgttttt 58440taaaagtctg gctttaaaaa aataataagt aaataaataa attaattaaa aacaataata 58500agtctggctt ttgagcctta ccagcatgtc ttgatgctgt gataaaaagt gtaactaagc 58560cgggcgtggt ggcgcacgcc tttaatccca gcacaggcag aggcaggtgg atttctgagt 58620ttgaggccag cctggtctac aaagtgagtt ccaggacagc cggggctata cagagaaacc 58680ctgtctcgaa aaaccaaaaa aaaaaaaaaa aaaaaaaaaa aagtaactaa tgaatctcat 58740gaggtgaatc ttgaagcact gcataattaa tcctttattc tcattcattt gaacatcaat 58800attagtgttc cctattcctt atatagtcca ggggttcata acatataaat ttaacaaaat 58860acaaggaaaa tctacctcct ggtgagatta ccatggcaat cactaccaca agcgagcaac 58920gaaacgtgct gagtgaggag cctgtccatc agcccctgcc cgaaggtgca cacacttaaa 58980ccactcagag gctctcagag gagggtggga agacctacag aactactcca ttaattagct 59040cctaagaaaa ggcaagggaa aatcgatacc attttgggag agagggtagt tgaaacaggg 59100tctcatttat cccagggtgg tcttgaacta gctgcacagc aaatgatgtc agcctatgcc 59160tgaccacttc ttcctttctc cccatcttcc cttctttaga gtgtattata cgcagcaccc 59220tacctctgag ctatatccac tgtcttttat tatctttttt tgagacatca tgaagcaaag 59280gattaagtgt ttctggtcat ttcagggtat ggcattgctc tcgactcacc ccagcccgtt 59340gtcctttccc tctgctgtgt ggggaccaca gtggaaaaat atgacaagca gctctgtctg 59400cactggaaga ggccttacac agtcattctt gacacctcac aacacacaac tacactgaca 59460agaaacaatg ttccaatgtt aactctatac tctttccata aaaacatata tattcatata 59520aatacataca gatatataca tacatatata tctctcaaat aattttgaat aaaagaatat 59580tgatattcac ttacattgtg ctattcttag gaacaagatt taagagaaat gattttaatt 59640aaaaaattta tttatgtatg tgtatgtatc tctgtgcttg aggactgtgg tgccctcagg 59700ggccagagga gtgtttcaga ttctctgcag atggagctgt aaacaactgt aagtcactca 59760atgcaagtgt gacaaccaag cacggggcat ttgcaagagc agtgcacgca catctttaac 59820ttctaggcca tctctctgat cctcgaaaag atcatttttc agagtgtttt cctactattt 59880gtattattta ttaaaattaa tttatgcctc atgtgggcat acatgtaggt acatgcagct 59940atgtacttgt gggaagccaa aagagtggct ccccaaagcc agagctacaa gtgtgagcca 60000actttgttac atggctgctg ggaccccgac tccagtcctc acggttgcat ggcaggcact 60060cttaaccact gaaccatctc cagtttccaa tttttcacat tttactttcc aaccacttag 60120catgtaccag aatcacttag caccgtctac atgagtgtca catgccattc cagggacaca 60180gcaaaagtta gggtcaatta tgacctgaat ttaggaggtc acaacggtct tctctggctc 60240tcaacttcct ggcctatcag cctgaaaggt gtgatgggta acccaaataa agtgttgggt 60300cagggataag gaaggactct ctggggacag ccgggcagtc ccaaaatcac agcacagagc 60360agccgttctc agcaaccagg gattccaccc tctgaactct cttctggatt gcaaaaggtc 60420agtgcactga ctcacaaagt gtttggattt ttaataaaca ccactctttc aatctcactg 60480cgccgaggcc cgaactttcc actgtctctt aaacccatgc actctggaaa ccctccccga 60540agggccagct tttctgtagt taccctgaag gtccttgctg tctcctgact gccaacgaaa 60600acactaacac gggagtggaa actgtcttgt ggagcccatt ctgggagtgg acatttaaag 60660gggaaagttt ccaacttcct ttggttaaag aaaataagtt tccacaattt tcaaaacatt 60720ctcataatgt ctgaaacttg aaagctcttt tctttaggga gtaagtcagc tatgtttagc 60780tttttttttt agacattctt tttttttaaa catagacatc tcactactta atctttggga 60840acaagattta aatggctttc aaactgcagt gtattgacca ttaaacaaga acataccatt 60900tactgttgat ggctaaacat aatttttatt gctgacaatt atttgatgag aagttccttc 60960tccatggctc agacacccta ttgctaccat gtcctggaaa tcattggctt ttcctggttt 61020tttgcccttg ccacctcccc gtgtctgtga cttccgcgtt tttaactggt ccttttatct 61080tcttgccctt ctttctcccc aaatcttttt ctatctgtag tgaaggttcc ttattatgtc 61140ttaatagtca agcaatttca cagtttagtg acaaaaaaaa aaaaacctcc catttcttac 61200acatggtttc catatcattt aaaaagaata ttgcatctac ccacatgcat ccacatgtgc 61260tgtggcacat gtgtagagct cagaacttgg gagtcagttc tttccttctt cctagtttcc 61320gagacagtct tgattctgtt acgctgtgaa ctccaggcca gttgaccgaa tagctttcca 61380gtgattctcg tgtttctgcc tcccatctca ccatagaagt caaatgtatg actggtccca 61440tagcttgtct gcaaatgtgc catgacactc tattacactt gggcctcagc ttcctagcag 61500aaccaaaata aaatcccaca tcctggcatg tgcaaccggc cctggccatt cctgtcaccg 61560tcactccgtt ctctctccct tcttagccct ggccatgctg accttctcct tttgactcgc 61620ctcttctctc tctggccttg cattatcttc cttcggctta gaaggctttg tctactctgc 61680ttaaatcagc ctatcacatc acacagattg catctttcaa ggtccttatt aagagttgtg 61740tgtctctcct catcagaatg atattgaaag ggacagtttc ttctgctgct gcatccccag 61800gacctcaacc agtgactaca cagtaggcag tttatccata tttgctgaca gagcctctgc 61860aagtctctcc tctacaatgt ccctcaagcc ttacctcaaa tgttctatct tgagtaaggc 61920atttatgtct atgtgagcac tagcatgaag tctgaatcag gaaagctatt gagggttttt 61980ttttctttag tgtcccaggc ttctttattt aattgataag tgacagctta atcaacatga 62040gcactcaact tcatcttcat ccatcacctt gtgctcctat cctcctcagc aaatgaagaa 62100cttcatcccg aagccctggt ggcacagcct ccaagttctc ttggtctagc tctttccctc 62160tctctgttcc ctacatccac tggcagggag agtgccccag tttaaaacgc atgggacagc 62220ctagagcagt gacagaaatc tctagagggg aagacttcat cctcaggaaa aggagcctaa 62280gagtagagta tggtgaaatg agggccccta cagcctgagg caccaaaatg ggggccttgt 62340ggcagaggaa agaacacaga ccgccccagg gaaagaagac ctgtacctca gacccctctc 62400ttgggaacag tcaatgcttg attatttgcc tgccgaacca cgttcccctg ctcccccaca 62460tgcacttctc catgcttcta cttcttttct gtgcctgcac tgcactaggg tctctgctgt 62520gcaaatctcc caaagggttg tcttttccat ccacttctcc agcaatggcc tggggtcaaa 62580tgttcctgat gctgagctga aggacctcat ttagctgaat accttcccaa agagaatgcc 62640cagggtgagc cctacattgg tctgagggta cctcacggtg ctcctcttct gcttgagggg 62700cctggcaagt tgttctagtt ctttctgcag gactttgacg tcctgggact ccttgggact 62760ggattccacc ttctccaact tcacagctct gaggtggaca gtacaggacc cagggaagag 62820atcctctcag gtgctcccca ttctagctcc tgcatggcac tcaggcttca atctccaggc 62880caacttaggg tattggtccc aggccaactt ggggttcaca atatgttatc cttccacagg 62940actcaatgga aagatcccca acacctccaa acttggtcca acctctggtc cagttccagg 63000cccacttgga gggcctaagg cgctcagcta tgtctcaggg catgcccagc ctggctctag 63060ccctcctaac ttcatcagtc ccacctggta gggatgagaa ggtaaagtct aaagccaggt 63120gtctgaccat ggggaagcat gggtacctca ggcagaggcc tgattactga gattcttgta 63180cacgtacaaa gtggggtgag gcagcagaca gcgtgtactt acagatcctg tgcattccca 63240gtaagctgga attttacaca ctacagtgtg ttcttcggtc tatggaagta tatggtgatt 63300acactgcttc actaatgggt tggaacgtat ctcttgttac atgatctata taattcagta 63360gctgaagaga ttaaacacta tcagccaggg atcatcctct gccatgtgct gtggactgaa 63420agttaggctg cgcttccttc ctctatctgc tttattacag aatcgcacac atctgaaaca 63480tggaaactga agggttaaaa gcgaataatc ccctcctttc cccagcctca ggtacatgat 63540gaggtttgca tggatgcggg tcataaatcc attttggacg ttaaaaccgc tggaagatga 63600aagggttctc atgtttttgg aacacactgt atttacatat acaaaggcat taaacgatac 63660atgggaaagg ccctgtgtgg cacgactctg gtattctcta actgccagca ctctgaatgt 63720ttgcggcccc agagaacctc ggatccctgt gggcaggagt cacgtttccc tttcccgctc 63780tgttgctgct gagccacctg aacaagaagc ctttaaagac aatggacaag accccagctg 63840acccggcaga tcctcagctc cagatgttgc ttttgcctgc aaatactgga gcctcactca 63900caacaaacac tcactgaaga gagtctacgg aagacagctt tgttactgcc attagcatga 63960atttctaaat tccacaacca ggaagaaaaa cccaactata tagtgaattt ttcttctctc 64020tagaaacaga taaagaaaaa tgaaaagaca taaaaaacca tgaagtgtag aattacagct 64080gactcttctg atagctatcc acatgcctac cctcacacca agggaccata ttcttcacca 64140gggctagcac tagactagca gggacacata gacagcttat gtagtaagct atctgtagta 64200tcaactacta aacacatatt gataggaagt ccctcaaaaa gcaaatataa tacaaatgaa 64260accagaactt actcatcttc cacagtggac acagtttatt tgagatagga tcctactctg 64320tagtcttggc tggcctcaaa ttcattatgt ggctaagggt agccttgaat tcccagcagt 64380tatcctgcct ctgcattcaa gtgttaacag ttacccacag gaacagcttg ggtgtgctgg 64440atcccagtag gcagaggcag gcaggactgg tctacatata gttccaggcc agccagaacc 64500atacagtgag accctgcccc caatttcttt cttttataac tatttattta ttggggaggg 64560caacacatgt atagtgtgtg catggagatc agagctttag agcacagtgg ccatnnnnnn 64620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 64680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcttta gtacgcattg gcaagctcca 64740tgtgctctaa agaaggccca cgctaggcga cgaggggaat ccacttacat ggcacagtct 64800agaagatata tagagtgcat tgtgaaagca agggacatac atgattctag agtgtaaaaa 64860tgatgtacac tcacagacac acagaagaca cacacacaca cacacacaca cacacacaca 64920cattattatt attatacgta tataactact aagaaagatc agtcaagcca ggtgtggcaa 64980caatgctatc atcctagtac ctgggaggct gaggcagggg acatcttgtt tgaggccagc 65040tttgggccac ctaggaagag actttgaaat cactaaaaat agacaaagca aacaaccctt 65100aaaagatctg ttataatgtt ttgtttccta tacgcaaatc tttaaagtgg ttcccttaaa 65160aacaaaaaca aatacaaaca aacaaggaac ccagcacagt tacttgcaac ttaataggga 65220aaaaaaggag aggacaataa gactctatgc tggaggtaag agacagcttc acacagaggc 65280cagaatggaa cggagagctt atacccagca tacttaggct gctggtgaga tccatggtgg 65340gggtggggga aatctctgaa tagaaagtcc ctgactgaag acagcagtgt tccacagctg 65400cccaggaact ggacgttcat cacaggcagg acactacatt ttaagcgatc ccgctctatc 65460agtacctgtt gcctgacccc ccccattccc ttaagagggg cctaattatt ctaataatat 65520gttttagttg ctaaacttga cctaattaaa gagctttgag gaggccgctc ttcttgagaa 65580acacaagagt aactcttctg aagggctacc atcagaagct cagaagctca ctgggccatg 65640ccacccacta attaaaatgc ccaagagaca ttcgtctggc agtaaacact aattaccaaa 65700acacatcaaa gcttctcaaa caagacagaa tgattcattt tgtgatttcc aaaataaggg 65760attttctttc atttaaaaaa aaaaaaaaaa aaaccttctt tacaaatgtc taagtacctt 65820atttttctaa aatagttgga aaattctcaa acttgttata gagttcaggc tagtgtagtg 65880cagcctagtt ctgttttatc tccacaatga cacattggtt gtccttggat aaaacattta 65940tactgccaac agcctcacct ccccttgctc tgttaaatgg aagccaactg tcacaagcca 66000ggaccaatga aaaagctcct gcatttgggg ataaacttgt actcttaaga gttaccatat 66060ataacaaatc ttgaattttc aacatttaaa aatacactta ttacatgttc actcagcgcc 66120atggcgggga agagttaaga attttgtgaa gagctccaag ctctccaaca gctgcctgtg 66180ctgctcagaa ccctcatcac tctggggtcc gggcggaagg catcagtggc agcatactgg 66240gtagcctgga atacgcacac cacccagaac tgcgactaca aacatccaca cactactgct 66300gtaactgtgg gtgtagctgt ggctgtcact taagcagctg cggcacatca ggcttcccca 66360agtccagcac ttgactctgc tgctcctcgc tgggctctgt gctcctctcc tccagctctg 66420tcttctgggc tcatgtgcat tcacacctta gcctcagctt ttattttcat gcactgggct 66480ccccccaccc attagtttgc tctcagtcta atatcaggtg ggtgtgccag gaaaccaatg 66540gcctgcagtt tcattccctg tagaaacctg cctttcagtg gtctgctgac aaatggttgc 66600tttatgttcc tacagactca gtgggcagga agcaaatatt caagtccaga tgggatattt 66660aacctctgag ggagaaaccc agaacagtag ccagcctaca cttacacagt cagaggatct 66720gaatctacag tggggcactt tcacacagtg aaacagtcaa agaaaagcag tctggacttc 66780agcaggattc cagctagaag taacttcctc caggccattt ggtgagctag ctctcctgtg 66840tcttggctac caatgccagc cctgatagag tctcaactgt acccagacct tggcttctga 66900tcagcttctg attcttgccc ttccttccct cagcagacat caaggagaca cctgctatgt 66960aaagtctgcc atgccgacct ttcccagcac taactggcag cttattgttg aaatgtggtg 67020ctgatttaag tgttctttac tgtaacatgg ctccagccag ctaaaacaga aatgcatttc 67080cttccctttt gaagtcttat caaaagtggg gcagaggatc tcttcccact taccagtcat 67140ggcaggtcac ttgccatggg tcctcaggtg ctcgcccatg aagtctttca aacatcagtg 67200gcactctgag gctcacatac cctctgaggg tgacagaata atgaatggag tctgcagttc 67260aaaaacacta cagcactagc acacagacct gcctcagaat tcactctcac tctgtcccag 67320tctactgggg caccaagtgg aggtgggata tgctgtggat attcaagttc gaacctttca 67380aagaataaaa cacacaggcc agtttttgct tgcctagacc agcagcctca ctggcgtctt 67440agaggtatgc cactaataga acagaggaag ggagctaaca tttcttaaaa taagtcactt 67500ttccactctg tgcagactgt ctccttcttc agagctgatg gaaaccacgg caggtcaagc 67560aatgggcaaa actgtttcct gagatggaag gtgagcgcag cctcacctct gctggtcttg 67620tgtggtggcc tgaaaccatg tctgcccttg caaactaacc agagccagca accctttaga 67680gtgctacttg gttctatcat ttgttcttcc tacagccctg gaaacaactt cctgtttatc 67740aagcaaatgc ttctttccaa aagagccagg gtgaggccaa aataactcaa gctgcccgca 67800agcaggttaa tgagagtgct cactttgctc tcctattgtc ctcagacgtg aaggtcaggg 67860tgccgcaggg tagaggaagc aggcttggtg ctctgtttcc tggcagaaac acacagaggg 67920cttctgggcg tgctaggggt tctttttaca gtctgtctag cttgccagtg gtccgtgttg 67980tacatttctt ctcccagact cacaatccaa cacagcaacg caatctatga gctccacccc 68040ccaaaattcc cagaagccag caacttctca cgctggtttc caccctgtct agcatcacca 68100attggggtga acagccacca gatttcaaga tctcttgata acatgcctaa gtattatctc 68160caattctgcc caaggctttc tgcatctgtt ctctaaacag tcacaagtca ctgggaagag 68220aatacaaaca ggtccccagt accttcctca atggctaagt cttacacaat tagagaagag 68280aagcaaggcc cagaatctga tatgggtgtg atatgtatcc gtgtcgtttt agcacacatg 68340cagatacaca accttctcac tgtaatcagg atgcagaatt actgccagtt tttcttcctg 68400gggagttact ccttggtgag aatgtctgca catgtgcaca caagtaagcc tgtcttactt 68460aactcctggc tccaaaagat acagagatat tctactaccc caaagttctc cctactactc 68520tgtcatgttc taacagatac agtgctcttg ccttaggacc gctgttctct cctgactttc 68580tcccagcaac atgtgaactt tgatcgcatt acagccttct ccgagtcctc ccttgactgc 68640cctttgattt tatcttagtg tgggtgttat actggcatgc ttctctgtgt atcacgtgtg 68700tgcctggtga ccacagaagc cagaagaggg agtcagagcc tctagaacta gagtcacaga 68760aggtagtgag aaaccacgtg gatgctaaga attgaactct ggtccccctt caagagcggc 68820aggggctgtt aacaactgag ccaactctcc actgctcctc tggccccaga cttttcacac 68880tttatcatct tctgactaat tacacatcta tgtagtttgt gtgttgtttg tctctcctac 68940taacaagtgt caacagggat ttgtgtcttc tgtattcatc actgcttctt gtacttgtgc 69000actagctggc acatgtgaag tacttgacat gtgtatgttg ttcagagaac agtggcatgg 69060gaaatgcaac cagtacaaat gtggatgcag gacccaccca ccaacagagt tctgaccata 69120tattttttta aactcacttt gccactaagc aggttttgta aactaagggc attttctgtt 69180ctttttaaat acttttgata cctagcccat ttacatgatt acatgaagca tgactgattt 69240tcacagggct ccgtatctct aaatccaaag gcctgtttgc ttgtgataag gcacctcctc 69300ctatcccaaa agtcttttaa taaaatgtta attgtggttt cctgcagtag aaacagatgc 69360ctctcatact gtcccagtgt atcagcacag gttggctagt ctgaatgatg cttctattct 69420tgagttccta cacgtagaaa aaaatatcat gcggtgtgct tccgtttgtg taggaggaac 69480agaaaagcaa tgttaaatga ataatgttaa tttaacatta aataaatatg actgcttacc 69540gaggtaggac ttatccctct cctagtgttc tctcctttag atagggactg tttaactcta 69600aatctaagac agagaatgtt caaggaaggt ctagaatctt gttcaaaggc aaaggagtta 69660ttgagaagtc aactggatgt ggttccgaag tggaataatt tgagtagtga gagaataatg 69720acacaggaca gggaggcagc tcagtgatag agaggagaga gaaagggaga agatggtgat 69780ggagaagaag aagaaaacct cagtggacta aagcacacca gccacattcc agtccatgga 69840ctcacagaaa agaaacaaac aactcaagtg ggtcccactg gacactatta gggtaccaac 69900ttgcttgaaa tctgaccttg ctacaatctc tagttttttt aaagcaaagt tctttaaaac 69960tgataaatgt agaaagcatg aaagaattag aaaactacca gtttgcaaca tttggggtga 70020actttgctct gagacatggt cttcctgtgt aacccaagct gacctgaatc aagcttgcca 70080tggaaccttg tctagacaca gactctgatg atcccatccc agcctcggca gtgtcaggac 70140tagaaggacg tgctatttgg accaggcgtc actttgtatt ttagcatgga accatgtctc 70200taggtgacca tgtgagagga gcagcttcaa aaactggcta aggtcagaaa gggattacag 70260ggacacagct aaacctgcag gttatctgaa catcactaaa gtggaaacca gtcactatcc 70320aaaacttgaa gtgaaccaat ggaagaggtg tgcagaatcc tgcctctcca ccagggaatt 70380ccacctacat ttaagcaaac tgtaaccaca actggcactt tacgagaaag tgcgagagct 70440aagaggacat gtttcatgat gagagacatg aacagccaaa tttagtactt acacatctta 70500caagacagtc cagctttttt ctttaacatt tatttattta ttttatttat ataagtacac 70560tctagctgtc ttcagacaca ccagaagagg gcatcagatc ccattgcaca tggttgtgag 70620ccatcaccat gtggttgctg agaattgaac ttagaacctc tgaaagagta gttggtgctc 70680ttaaccactg agccatctct ctagcccaac agcccagctt cttaagcaag agactacaaa 70740ggaaaaggaa

caaacaaaaa tgatgtggca agtggggaaa gaagggcttc cagattataa 70800agacatagta agacaggcat gtatcaagcc caagcatgct gtaaacaggc ttcatgggcc 70860gttctggtgg gagttgggat gaccaaatta ccacgacaaa cagaagatag tgaaggcctg 70920gctcccgagt ttcagaggag aatgagaact agattagaaa ctgggctaaa ggccatttta 70980tgatatccta gcaaggaatc tggctatttt ctccatgtgt tgagaactga gagaggctgg 71040acgtaagagc aatggactaa ttcatctggt agaagaaacg taaatacaga atagcatgca 71100ggctgtgaca tggctgcttc ttacagttct catccaggtc atctgtgagg gagtatcaaa 71160aggcaaagca gaaagatatg aaattgtgca ctttgagagg aaaggagttg gagtttaacc 71220ttactaaaag acagggctac aggattttta aagagattac agcaatctcc actgcaacta 71280caggagctag gtatgcccta agggcaagac cttagacact ggagactcca ctgtataaac 71340ttgaaaattc atttgaaaga agaaaccctc aacagagtaa gtcactgaag gaacccctcc 71400tcctcctgga aaacagatcc ctagggatgt gtttcgccat tagtacacag agactgatac 71460aactgtggtc cccagaggcc atgctccatc cactttgagc tggtagcaga cattgatgga 71520attagtcaca caatactgag ttctcaggca tgagagacac gggactgtgg gggtccaaag 71580atttaagtca cagttgaaga gtgttgctga ggccaggcca tgtgtggcaa gggagaggtt 71640cctgtaagaa agtcctgaag gggcactggg tgaagctctg aacgtgaagc cagaatagca 71700atgaagagac actgggaatg ccagggccat gggacaggca ctgaggaaag ctgcaggcac 71760ctggcagagc cggccgggag agaagccgtg tgatagacag aactgttgaa gggctggggg 71820ttagccataa cttgtggtac tcgggtggcc ccatcacaat ccccagatgc aggccacaga 71880gcttgaagac ttggtgtttt tactgctagg ttttggtctt gctttgaccc aattgctctg 71940ttctatgctc cattttcttc tttcaaaata tcaatgttta ctcagagctg ctgtgtattt 72000gacgtgggtg atttgtttat gattttatag ggatttacag ttaggaggcc accttgagtc 72060ttctgtgact ctaggacttt gggctttcca atatcgttaa aaagcccagg agacttcaga 72120agttggactg aatattcaca ttgtagagag ccacgaacca agctaaggaa taaaaggtga 72180tggcttgaaa gtgctatgtt gaaaggctct gggatcacct tggagacaaa tttctgggca 72240tatctgtaag gaactttcta gattaagtta cataaaatgg gaagtccagc ctaaggacag 72300agggacccac accagcattc cccagtgcct gcctaccaac tctgggacac tgtgacagtg 72360cctgtgctta ctatgctccc tgccatgatc aaatgtaaat tggaataaac catcaagttg 72420cttcttgtcc aggattttgt tacagaaaca agaaaagtag taacacagaa ggacaaaggg 72480gaagaaaact ggaaagagca tcaaaattag caagaaggta ttcggatgca tacatgtaat 72540tgcaatcgtg tggggtagag aggcaagagg attgcaagtt tgaggctatc ctgagctata 72600catttaaaat ttaaaaatca gaatggcaat tctactcaga gccatggtca agcccctggc 72660tgtatgcaca caacgttaac actcggaggt gggcttagca gagagcagca gccaggagtc 72720actcagaggt tccacccaga agatcctgga gttctccttc aagttaaaaa gagcagcttt 72780gttgaatatt ccagaaaact atccctctgg agtaagggct acataagagt aaggataaaa 72840tggaaaccac aaactggagc tgcgacaggt tcagtttaat cacctgacag aaccaagtgc 72900tactgaaggc aacaaccaca atcactgcag tgatcgcccc tcctgcccaa cacacagtaa 72960aaatgacaga acggaaaaag cagaactggt aggatggttg gcaggcagag gtgcttgctg 73020tcaaggctgg tattcacatg gtggaggggg gaactggtac ctacaagctg tcctctgatc 73080tccgtgtgtg ctctggcaca cacacacaca acaaatgaaa caagtgtttc aaatagtctg 73140tgaaaacagg ggcaaaaaga ctgagagacg tggatgaaca aagacttagc tatgattgct 73200ttgctaaaga acactcacga aaaggagagc gaagggataa aaagagcctt taaggtaaga 73260cactcactaa acctcgtcta aagctgcaaa atatcttaaa ccaagaagct actagaagct 73320gggtggggag tacagcattt gggagcctga ggaagaagat taagtgttcc aggttagttg 73380gggctaacaa ctagtgagac tgtctcaaaa acaaaagcaa aaagcaaaca gtattagtgc 73440tctttttttt gttttgtttt gtttttccag acaggatttc tctgtatagc cctggatatc 73500ctagaactca ctctgtagac attagtgccc ttaagaaagg tgttgtgatt aagctgttta 73560aaatccttta cacataagaa aaactgtaag tatttatgac taaaaaaaaa tgttccttga 73620atttaacttg aaatattcaa aactgaaact atagaacagt tggggaaaag aaacaacagc 73680accagcataa tgctggcgat tgtcagccct gggtgttggg ggagcggggt ttaccacgag 73740acccttctcc tgtacgtcta gttgaacatg cttacagtaa aaaggggttt tcactggtac 73800atcagtgctc actagtatca gcatcaggac cgaagcatgg gaactcaagg gtccatcacc 73860agatgaacac atgaaatgtg tcactgatga gtctgacaca tgaacatcat aagtgacata 73920agctggtccc acaaggacaa atacggggtg attccattta tataagtccc taaagtagtt 73980aagctcaaaa catcaggaag ggtgatgctt tgggggaact gtagggtggc agaatagtgg 74040ccagtgatta acacctcgag tttccattct acagaatgca aagtttcaga tgacaggact 74100gcagtgtcat tattaatgtt actgaaaatg gttaaaataa ttctgtgtac tgaatatttt 74160acaactttaa aaaaatggaa tgctggctgg aatatcttca agacattttg aagatatact 74220gaaatgtgac atattaattt caaataagaa attaaaaact gtagatatca ataaaggtta 74280taattaaata tcttttcatg tctttattac tagaccaagg aaaactacat tttcctaaaa 74340gggttaaaac atagtttgaa aaaactctca ggaaaagtgt gtatgtatac agatttatat 74400tgacacatac acatgtatat ttatgtacac acacaaaaca gaaaaaaatt cctttcgaac 74460atacatatac taaattcagc aaaaaatgtt tggcttgggt atgatgccag cactcagaag 74520acaaggggat ggtgaactca atgtagaata ggctacacaa tgagacctta tctcaaaaac 74580cttccatgaa aaaaataaat attttaaaaa cccacaaata gtgactgatg agactgtgca 74640agcatgagga tccaagctca tatccttagc acccatgtaa aatgttgctt ttggtacatg 74700ccagtaatct gaccccttgg aaggtggaga ctggaggatt cctgaagctc actaccagag 74760tcctactgaa atcgtgagtt acaggtccaa tgagagactg tatttcaaaa ctatggtaga 74820gagaaagaaa agcagccaac atcattctct ggcctcggcg tgcactgagc tcacatggta 74880caaataacat cttgatcaga aaactgtcca tgacaggatc ccccatggag gagctagaga 74940aaggacccaa ggagctgaag gggtttgcag ccccatagga ggaacaacaa tatggactaa 75000ccagtatccc cagagctccc tgggactaaa ccaccaacca aagagaacac atggggggac 75060tcatggctcc actgcatatg tagcagacaa tggccttatt gggcatcaat gggaggaaag 75120cccttggtcc tgtgaagttc tgtgcccctg aataggggat gccagggcca ggagtgggag 75180tgaggngttg gtgagctggg gagcaggtag ggggtggggt tctcagggtt acctaggagg 75240agagacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 75300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaaa cccctgtaga 75360gcattctccc ctctcttcta catcctcttc ctccattact attacaatgc tgtgcntgtn 75420ttttgttngt gtttggggtn ntnnnngggg ggcgtccatg tagtacatct attatgacca 75480tgattacgaa ttatggccac tgtgctctaa aggtagtgtg tgtgtgtgta gtatgtgtgt 75540atgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtatgcaggt atggacatgt 75600catgcatata tgtggagatc agaggacaac tttgggaaag tcaggtctct ctcctcccac 75660catggttgca ggacttaaac tcaaatcacc aagcttgtgc agcaaaatgc cctcccccca 75720ggaccgcctc ccactcccca tttttttctt ttaagaaatt aagacccctt tgtcctctac 75780ttcatcttgt gccttcttct ctatgctcag gtttaggctt attttttttt tttaatttaa 75840ttttgttttg tttgagacag tttcgctgtg tagcccaggc tggccttgga atcacagtag 75900tcctcctgcc tcagcctccc aaacactgga attataagtg ggtaccacta ccacattgac 75960ttaagcaatg aacctcagag ctgtatgtgc taaaagggtt acggtgtgtg gatttttaaa 76020tctcggttta caaagagaaa ccgagcagga gcagccactc cagtggcttg agcagaggct 76080aatccatggg tagggctgac tcgcgagcct gggtgctttg ctcctgcctc actgatgatt 76140cctgaccttt cttctcaggt cttctggaac acaatcgccc ttctgggaca tggagccgcc 76200ttccttcccc aagaaacaat tcatcgttgt tctgctagca tcccaagaca tttgacaagc 76260ggcagcaact gggctgaaag ctgagtctag gatgaggtag ttattttaaa gaccctgtgt 76320ttgttaactt gccttcctcc ctcccgccct cctgcccccc ccccccctct tctctgtctc 76380tctctgcctc tgtctctatc tttctccccc accccctcag ggattcacgt agcccaggct 76440ttcttcaaac tccctaaata gtacaggaat cctcctgcct ctgcctccca agtgctgtaa 76500ttacagttgt gtcccatcat gcctggaatt tctgtctgtg tgacagcgtc tcatactaca 76560gcccatgctg gcctaggatt tacagcagtc ctgcctcagt ggagtgctgg gcttacaggc 76620ttctggtgtg atttcccaga atttattcag gcagtccttt tctccaggtg ttccttttgt 76680gaggccaaat tctggagctg acctgggaaa gcaggcagag agattatggg tccaagatgg 76740agctgagttg ctgccatgcc tgggggtgac tggggctcac cgtcttacct gcttggcttt 76800tcttactcag ttgagaatgt atttgtctca ggtttgatgc aactgttctg gggcttacaa 76860acttcactct ttcagaaaac aagggcatgt atgctacctt cgtgttgact tgatcctcaa 76920atggctcctg tgaacccagg gacagagtca gctgataaaa gacaaacccc cccctcctct 76980gcatcacagt ctgagcccat ttcttagtta agaaatcaca ggactttatt ctgccccagg 77040taaaaatggg ctctctaggg ctccatagat gatcagtggc taagagtggg tgtgtctttt 77100gcagaggaca catgagcttg ggtcccagca cccacattga acagcttaca aaccatccaa 77160ctccggctcc tgagaatcag gcgtctctga cctctgagat cacctgcatt caaatgtaca 77220tattcccaca cagatacaga cacacacgca catggacaca cacataaata cacatacata 77280catatgtagt aataataata ataattaata ataatagata taaaaatggg atctctagtg 77340cttagtaagc aaggttagga aatagtcttg ttcaaaattg ctctgatggc aagtgagata 77400ttagcttagt tggtaaggcc acctactacc tggtgatggg tgtagagaga tgattagtgg 77460ttaagagcac aggctgccag ctcacaaatg tctgttactc cagttccagg ggatctgatg 77520cctcttctgg cctccacagg caccaggcac acattgcaca gacataatac atgcaggcaa 77580aacacccatg catataaaat aaaataatac agaaaatatg tatttgtttt taagattatg 77640cagttcctac cgggagcttt tcgttacttc caaagcgccc ttgggactga gttctccagt 77700cttggaagat ctgcttgctg gtcacttcag attgtaacct gccttggaag accccagctg 77760tgttgggggg acacctattc ttcccttttt tctccaggag cttgagaggt ggcagagagg 77820gcatctggga aagagtgaca gctttaacag cacttgtcag ctgggcagca cggagtggct 77880atggactaga gaaagccaga aatgcgtttc tgcatttccc aagagaatca aaggggtggc 77940aggccccatt gttctctttc ccctgacgga ggagttagga ccctaccctt cccagacagg 78000atatctctgt gtagcctgac tgtcctggta ctcactctgt agaccaggct ggtgtcaaac 78060tcacggagat ctgcctgcca aattctggct tttttttttt ttttaacagc atcttgtagc 78120ttaagccaat ttgtagccaa ggaaggatga ctttgaactt ctgaccctcc tgcttccacc 78180ttccacgtgc tgagtccaca gacactcacc actatacccg gtttttatag tactggaaat 78240ggaacctagg gcttcacgct tattaagtaa acactgccat ttgagcccca gccctggtta 78300cctcttgacg ggaaagcttg cgcactttac cctggctgtt gtagcatcat taaacttggc 78360agttctcttt gtccccacaa aggctgtccc atgctttgtc tggctcttct catgatgtgc 78420atgggtcata gaccaccaac cggattctgt tcccttctgc ctcgacacgt gctgtgcgga 78480ttggctgcac agggaacaga agattctact gggggtgctc tccctccccg agccaaccca 78540ggtgacctga acggaagggc cccgtgacgt gacgtgacgt gggcttgcct ccttgccctc 78600tgtttgtggg tctgcctaaa tgaaacaata tattttcggg tactttcaag tgagaatttc 78660tttattgctt ctctagaaag aaaaaaaaat taaatttatt ttatgttact ttttcgtatc 78720tctcagttct catgctgtga tttagcaggg agagcgagtc acctgctgcc acaggttagg 78780agcacaggga acacactgtc tcccctgacc cccagcacct aggggccgca ggttgagata 78840gggggtctgg gggaaaggga gtttggaagt ttgtttggaa gtttgtggct tcttttgtca 78900ctagactcgt gatcgggtca ctttgagatg gacatcacct cttcaatcta cagctgtggg 78960acacttgcaa ggcacatggt agtgcccctg ggttcaaggt gggcttccta attgggtagg 79020gatgaggtaa aggactgtca ggggccatag agggccatag caggtctaag atagtctgta 79080ctcccgtgag accagtaagc ctgggtcttc ctagcttgca tgggggtggg ggtgggggac 79140actatgtctt ccaggaatgc acaggggaca gggctgacct tgaatttagg atcctcactc 79200ccctaccagt ttgtcctttc tcaaatctca ggtgacatca aaggtcaggt taaagttgaa 79260ctagagggaa gtctaaaatt cctgaagaac acgccctttg ctctactaaa gagctgtttc 79320tctgagatga cagatggggg acttcatata gtacctgcgc ctgggaaata ggcccctcct 79380ttccctactc ccacccacac agccctgcct cctgggcatg agttcggtct cagctctgcc 79440tactcacatg actccctgac tgctcttgtc tagaccaggg aagagggcat gactccacac 79500taggacttgg gtgtcctggt cctggctgtc atactccaca tgtctcagct gtcctcttgg 79560tgttgctctt ctgccctggg tgtgacctgg aagtattgtg tatgtagctg agggcggact 79620tgaacttctg ctcttccttc gtctacctct caagcgttgg gattacaggt gttgcatcaa 79680tcaccatgcc tgctttatgc aatgcccggt ctgtcatggc cctggtgggg acagaatgac 79740atggctcctg cccctgggac agggccagca ggcatgggcc tccatgattg ttgatagctg 79800ctatgggcat aaggcttatt tgtatgaata atgtacatat tttaccttcc tccagccagg 79860acaatcagag acagcatgtg tctgcgaggc aaaggtgtgg ctagtgtcag caaaatggta 79920aacactggga ccttcaagac agcccttaag gctgggttca aaaacatata acttctcaaa 79980aaataaggat gcaatatata tgaggggctt catgaatcta aagaaccaaa agcgggtgtg 80040ctgtgagctg gcttgtcaga aaaagagatc ctaagatgga gaaagagaga ttagggagtg 80100gtgagcacag gaggttccac ccagtacatt ttttggtttg tgcttacaag ggcagacaga 80160ttcctgagtt caagctcagc ctgggacaga gctaatttag acccaagtgt agtagaaaag 80220gtaatttcag gatgggttcc cacccaacta gtttatgctg gtctgtgatt agcagaggcg 80280ggcaggtctc tgaattcttt cgaaatgtta aaagaaaatg tgtgcttgcc atctcctaag 80340aacttagggg ccggggttat gggatactgg ttcataggat aatcaaatgg aacctgggta 80400aataactggg ttgatatgta aaataaaaga ctgggcttag gatctgcaag agaggagcta 80460tctggagaat gttttagaat agcaagagaa agaactgtct gcagcaacaa agagaggtag 80520cagcagcagc agcagcagag agagagagag agagagagag agagagagag agagagaaac 80580ctgtctggag atctccagag aaagcagatc agagagagag gaagcggact agagagaaag 80640caggctggag agctgtcttg agcagaacat atctgctagc ttcttcctac aatctgactt 80700cttgagtcac tttcaccact cccagacacc ccttctctca ggaccccctc caagccaagg 80760ctggtccttg gcaaggcaat aatggacttc acgaatgcta gacaatcaat ctgtccacta 80820agctacatcc gagcctcagt cagccccttc attttagatt tacttattta ttttatgtat 80880atgagtatac tgtagctagc tgtcttcaga cacaccagaa gagggcattg gattccatta 80940cagatggttg tgagccatct gtaatggtgt gtgtgggtgc tgggatttga actcaagact 81000tctggaagag cagttagagc tcttaaccgc tgagccatct ctccagccca gcccctccct 81060tcattttaaa agcctggacc gcagggtcaa aacaagaagg agggaaggca gaggagggac 81120ggtaggcagt ttggcaccct gcagagtgat cctggctagc cccttggagc ctggcaatgc 81180ctgaaggagt gtgctgaagg ggaggaacca agcgtgcgct ctgtgttgta ttctactggg 81240ttggtggccc caccactggg atctgactgg gacagagatg ctcctgcatg ggactcaaca 81300agggctctgg ggacaagggg ctcagacttc cctttatgtt caatgccatc ttccacccca 81360cccccaccca taagtcctac ctgaatggga aagctcagcc tcagggtttg tcacggtcca 81420gggcttgaac cactctctct ggtttttgct gaatggcatc cccccaggct tgtggcactg 81480gctgtgtaca ctggaactca tgctccctaa tggagcctcc tcgagcagct gcaggcggta 81540tttcacgtct gttggggaga tttcctcctc ttccagatcc tcagggacag ctctgtgctg 81600ctgctgctgc tcctgctgct gctgggaggt tcactcggtc agaggggcca ttactgggtt 81660aagtagggga agtgagtgtg gatgtgtgtg tgtgatgggg agagaagtct cctaaacact 81720tgccagtgtc ccctagaaag ttacttgtct gagctacaat gtccttactt gcaaagtcaa 81780taaaatatgc ccgctttctg ggttccctag gagagcagag ctaagggctt agcattatgc 81840ttggcctgag taagtggcta atcgtctctt tctcttaaaa tatttatgat cctgccagac 81900agtgctgcgt tggtggcaca cggggagact tggttggagc ccttagcttt acctgaccac 81960tggaggcgct gtcctcaagt ccagtgggaa cagccaggga ctctcgctgg atggaattgg 82020ataaagagtc atcaagcaag aaggatcttc tgggtccagg agagagaatc tctagagtag 82080ggaaaagaac tgaggaccat ctcagccctc ctgcctgtgg gccgctttta gctctccatc 82140accctcccag gggaaagcaa ggaagggagg ggcaatgtcc agatgggtcc agttttaatt 82200tatttgagat tagattagtc tgagacctgg agtggaaggg ttagagtgga atggggctgg 82260ctctctgcat ttaggggcac atggcaaagg caaccttcct tccttcctcc cttccttctt 82320tccttccttc cctctttcct ccatccttcc ttcctccctc catccccatc tgtcttctac 82380tcttcctcct ccctccctcc ctccctccct gccttccttc ttttttgaga gacccataga 82440acccaacgat agctaggccg taactatcta cataactaca tgcagtccat gattaattga 82500gcctcctgtc tgtgccgccc gaccgaggga acgacacgac aggcgctgat gcattcatag 82560atgtaaaccc gaacgccctg caagctaggc acttacgttc aagacctaca tacctaccta 82620cctacataca cagatataca atccaatcat tggattggtt ctttctttcc aactagcctc 82680cctttaactg tgggcattga ttaacctacg acaatacaag atggatcctt tgtcccctgc 82740taggccaatc aaaaacaccc tgtccacaaa ctttgaaaga ataacagaag ccaccccttc 82800tttttctagc gggcgaaaag aacaaaaaaa ccaccccaac gctttcggtt cctttccttt 82860ttttggctcg gagcccgcaa tacctcccca atcacccctt ctagaaagcc cctcctgggt 82920cttccctaac tgccctggaa agcaaatgaa agggagcgct cgggctggga acataatnnn 82980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 83040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat atagacgatc tttatgacca 83100tgattacgaa ttatggccac tgtgctgtgg agccaatccc agcatgggtc gggtgtctat 83160aaggaaaggg tcacagagac accatgtgaa tacacctgag aggtagccag gttatgctcg 83220caggattaaa acagactaat aaatgctcag atactgtacc ataaagtata gctgggttaa 83280gtaaatctct ctctctctct tttttttttt tgtttttggt ttttggtttt tcgagacacc 83340acccggcggt ggttaaataa atcttatagt ctctgtctca tttatttaga agttggctgg 83400gacagataaa aattatccct tttaaaactg aactaacaac tgggaaatgt tatcttaagt 83460aagtgagagc attcctagta agtcattcac acttcagcag cttcctctta aattagcact 83520ttgtcttcaa tgcctatggg ttttgctggc atccaggaag cttttgatca agtgctgggt 83580ttaagagcac atgataggaa gtttgaggga atatatctca agaaattata ttgggggagt 83640tttgaattat gaatttagtg atttgccaaa cttactagct tctcaattct attggaacat 83700taggcagagc cacactgtca tggctgccct tttacaacgc agtgctaagc cgcaatattc 83760atttcaccga gtcagctaat agagcaaaca acacgcatgc agtgatgcac tccacttctt 83820ccaggtcaaa cttcccatct gcttcctttt ctctatacac tcaaatagca aagataaaca 83880tgtttctccc ctaagtcaaa acctcacaca actaaaataa gtgagcagga gaaatggcca 83940ttcattctct gcttgacttg gaatatgtgc gtgctgtgct ggacaccaca ttaaacaata 84000agagcaggtc acagctgtaa tgccatctgt ccatcagtcc attttcattc cctccttctt 84060tcattcagct gatctataaa tagttagtgc ctatgcagtt tctgtgggaa ctattctgag 84120aattggcaag caccaaaaat tacaaaatcc tggcttggga tatggttcag tatctcttgc 84180ctatcatgtg taagggcaat gttgactctc tagaaccaaa aatacaagac aaactcttta 84240ctgtcaagat gtactttggt aaggtcagat gtaaacaagt gagggccagt tagatggctc 84300agcaggttag gaaacatgat gacctgcgtt cagtccctgg aacccatatg aagacagggg 84360aggagaaatt ccataaagtt gtccaatgac ttcaatgtgg atgcatggca tgcacacaga 84420gcataaaaat gctacacact aatgagtgac tgagtatatg tcaattggtt ataaagacta 84480ataagaaaag taaagaaaaa aatgagatac agaatagtaa gccagtagga aaaataaagt 84540ttagttaaag ttgtctcaaa tgaggaaatg caggaaagat tctcaagtaa gtggaacaaa 84600gatgtgggac gagagttgca gaaagaaatg ggacaatatc tagaccttac agtggaggac 84660agaggacagc caaatgtgga aggagcagga gagcattggg agctacagtt ggagacaggg 84720ttgtgtgttt taagccctcg taagacactt ggtgagcgag gcacaagagc tgggtgagat 84780ggcagcacta gcacgatgtc cagagtggtt cagtctccgg tcccctaggc aaggtcagca 84840taaacacagc tactgagacc taccaggcag cttccttcca tcccagcatc tgcggagctt 84900tcctctgagc tcagcacccc tcctatggcc atgctgtaga gctgtgctgc tagaagctag 84960cagcttctct tgctaggctg cctctgccac cgaactccag tgcagacaga ctgtccttca 85020tcggaaatgc ttgggatcag tatttccaat ttcagggttt agttttattt tgaacactca 85080tataaatttt accagatagt tctcatctaa aaccccccag aataaaaaat gtttcaaaaa 85140acaaaacaaa acaaaacccc aaatcatatt ggcattcaaa agcttctgat ttgaaagcat 85200ttcccagtgc aggctcctgg tcaggcattc cccatgtgaa gcacgggctc atcaccgctt 85260ctagatcagc gacatagaag cacatctgca taactgccag caactacaga ggaaagcaag 85320cagtaccact cactctgcgc ttgatctcag caacttatcc agcacatttc acaccgggaa 85380cgatagagaa atcagcagtg agacttgctc ttctctgctt ttcaagcaca ctacgtctta 85440gaagtccaga gtctacacat ggcacactac caattaaaca ctgaactgac tgactatggc 85500caaagtaaaa tacttttagt tctttcaaaa tagggtgcaa ttaaatatga aagcactgta 85560cagtatggaa tcagaatgtg gccttgcccg gagaaaagat ctaacctccc cttggctttg 85620tggtggggtc tttattagcc tggagcctca gtcgtgttag aaggtctaat aataatgtag 85680gaacactagg aagaccaacc atatgaaatt aagcttggga ctctgggaca tcatgtcaat 85740tgtcctgaaa attgaggtca acagtatgga caatcaatca atcaatcaac caaccaacca 85800accaaccaat

aaggcacaca taaaataaaa atgtggatgc aaaggctgag cctccctgta 85860acaatattct gtgcatattg tcacacactc atcccaagac aaagcacacc caggcaaagg 85920acaatgaaga ctctgcaccc agcacttgtg ggcctgccgg ctttacaccg cccttggctg 85980gtttcaccga catcctctcc ctggaagagg gcacagcagt gagttctgag agtcttccag 86040cagtttatca cacccgaatg gctctggggg atttctcaac ttacacttgg tgccagagtg 86100cgagtgatct tggagaaagt gctttctcta tggcagctca cccctgcccc agcaaaataa 86160aaaatctctt gtacaatgct ctggctgcca ttcaaatgcc actgtaaact aacctaaatt 86220cacagttcag ttccacagct gaccttgctc catttccaaa gagcaacagc agcgtgtgaa 86280cagcagctca catgctggga gccatattcc tggactttat ctggccttct ggttcccaac 86340acacttggtt ttcaatttca cgtagacctc cagcctttcc tattctaatg cgtgatcatt 86400taaactggtc atacttgtat aactactgcc gctgaagcac agacatcaca agaaaaaaac 86460atacatgtct tcttttaaaa tcacaaaatt cctcctttga ccccatcggt tctccttgtg 86520atgtacctat ttgtgggcct ttgtatttag gctacatttg aaagtccttg aggtttttgg 86580acatgtgagt tactattttt tcaaaatgtc tgctattttc atagccattt tttcttacag 86640tttctgcctc tgtctctaca ccctcctaat actgtcactg tgcacggaat atgctctgtc 86700tcctacagtg cagaggatac tctgcctgtc tcctcccact gtgcacagga tgctcctgac 86760tgtctcctcc cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg 86820ctcctgactg tctcctccca cagtgcacag gatgctcctg actgtctcct cccactgtgc 86880acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc 86940cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg 87000tctcctccca ctgtgcacag gatgctcctg actgtctcct ctcactgtgc acaggatgct 87060cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac 87120aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca 87180ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc 87240tcctcccact gtgcagagga tgctcctgac tgtctcctcc cactgtgcag aggatgctcc 87300tgactgtctc ctcccactgt gcagaggatg ctcctgactg tctcctccca ctgtgcagag 87360gatgctcctg actgtctcct cccactgtgc acagaggatg cgccttacag tctcctccca 87420ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc 87480tcctcccact gtgcacagag gatgctcctg actgtctcct cccactgtgc acaggatgct 87540cctgactgtc tcctcccact gtgcacagga tgctcctgat tgtctcctcc cactgtgcac 87600aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca 87660ctgtgcacag gatgctcctg actgtctcct cccactgtgc ataggatgct cctgactgtc 87720tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac aggatgctcc 87780tgactgtctc ctcccactgt gcacaggatg ctcctaactg tctcctccca ctgtgcacag 87840gatgctcctg actgtctcct cccactgtgc acaggatgct cctatctgtc tcctcccact 87900gtgcacagga tgctcctgac tgtctcctcc cactgtgcac aggatgctcc tgactgtctc 87960ctcccactgt gcacaggatg ctcctgactg tctcctccca ctgtgcacag gatgctcctg 88020actgtctcct cccactgtgc acaggatgct cctgactgtc tcctcccact gtgcacagga 88080tgctcctgac tgtctcctcc cactgtgcac aggatgctcc tgactgtctc ctcccactgt 88140gcacaggatg ctcctgactg tctcctccca ctgtgcacag gatgctcctg actgtctcct 88200cccactgtgc acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac 88260tgtctcctcc cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg 88320ctcctgactg tctcctccca ctgtgcacag gatgctcctg actgtctcct cccactgtgc 88380acaagatgct cctgactgtg tcctcccact gtgcacagga tgctcctgac tgtgtcctcc 88440cactgtgcac aggatgctcc tgactttatc ctcccactgt gcacaggatg ctcactgtct 88500atctcctcac gctctgcgca caggatgctc ctgattgtct cctcccactg tgcacaggat 88560gctcctgact gtctcctccc actgtgcaca ggatgctcct gactgtctcc tcccactgtg 88620cacaggatgc tcctgacttt ctcctcccac tgtgcacagg atgctcctgt ctgtctcctc 88680ccgctgtgca ctggatgctc aagataaaat cctcccactg tgcacaggat gctcctgact 88740gtctcctccc actgtgcaca gaatgctctt gactgtctct tccaatggcc cagggatgct 88800cctgactgtc tcctcccact ggccaaagaa tgtccctgac tgtctcctcc cactgtgcac 88860aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca 88920ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc 88980tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac aggatgctcc 89040tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca ctgtgcacag 89100gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc tcctcccact 89160gtgcacagga tgctcctgac tgtctcctcc cactgtgcac aggatgctcc tgactgtctc 89220ctcccactgt gcacaggatg ctcctgactg tctcctccca ctgtgcacag gatgctcctg 89280actgtctcct cccactgtgc acaggatgct cctgactgtc tcctcccact gtgcacagga 89340tgctcctgac tgtctcctcc cactgtgcac aggatgctcc tgactgtctc ctcccactgt 89400gcacaggatg ctcctgactg tctcctccca ctgtgcacag gatgctcctg actgtctcct 89460cccactgtgc acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac 89520tgtctcctcc cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg 89580ctcctgactg tctcctccca ctgtgcacag gatgctcctg actgtctcct cccactgtgc 89640acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc 89700cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg 89760tctcctccca ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct 89820cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac 89880aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tttcctccca 89940ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc 90000tcctcccact gtgcacagag gatgctcctt acagtctcct cccactgtgc acaggatgct 90060cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac 90120aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca 90180ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc 90240tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac aggatgctcc 90300tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca ctgtgcacag 90360gatgctcctg actgtctcct cccactgtgc acaggatgct cctgactgtc tcctcccact 90420gtgcacagga tgctcctgac tgtctcctcc cactgtgcac aggatgctcc tgactgtctc 90480ctcccactgt gcacaggatg ctcctgactg tctcctccca ctgtgcacag gatgctcctg 90540actgtctcct cccactgtgc acaggatgct cctgtctgtc tcctcccact gtgcacagga 90600tgctcctgtc tgtctcctcc cactgtgcac aggatgctcc tgattgtctc ctcccactgt 90660gcacaggatg ctcctgactg tctcctccca ctgtgcacag gatgctcctg actgtctcct 90720cccactgtgc acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac 90780tgtctcctcc cactgtgcac aggatgctcc tgtctgtctc ctcccactgt gcacaggatg 90840ctcctgactg tctcctccca ctgtgcacag gatgctcctg actgtctcct cccactgtgc 90900acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc 90960cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg 91020tctcctccca ctgtgcacag gatgctcctg actgtctcct cccactgtgc acaggatgct 91080cctgactgtc tcctcccact gtgcacagag gatgctcctt acagtctcct cccactgtgc 91140acaggatgct cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc 91200cactgtgcac aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgtctg 91260tctcctccca ctgtgcacag gatgctcctg attgtctcct cccactgtgc acaggatgct 91320cctgactgtc tcctcccact gtgcacagga tgctcctgac tgtctcctcc cactgtgcac 91380aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca 91440ctgtgcacag gatgctcctt acagtctcct cccactgtgc agaggatgtt cctgattgtc 91500tcctcccact gtgcagagga tgctcctgac tgtctcctct cactgtgcac aggatgctcc 91560tgactgtctc ctcccactgt gcagaggatg ctcctgactg tctcctctca ctgtgcacag 91620aggatgctcc tgactgtctc ctcccactgt gcacaggatg ctcctgactg tctcctccca 91680ctgtgcacag gatgctcctg actgtctcct cccactgtgc agaggatgct cctgactgtc 91740tcctctcact gtgcacagag gatgctcctt acagtctcct cccactgtgc agaggatgtt 91800cctgattgtc tcctctcact gtgcacagga atctcctgac tgacaccaac tctggaagct 91860gcctttagtt ccttcttctc atctctgtac ttgagtgtgg caatcttact gatctatcct 91920tgagtttgtg tttcttcttt caggcagttc aactctgttg cggagacttt ctaatgattt 91980acttatttcc cagcccaata attttttaaa acaatgtctc ctgttgatat tctttatctg 92040aagtgacatt atgattcctg cctttaccgt tgttgcttca ctggtgagcc cctctgtgtc 92100tgtgatcatc tttatgtttt cttctggtaa atctggcatc atctacctgc ctattatcat 92160tttcttgtgg tctgcatctt gtggtccagt aactgggagt gcttttgttg tttgggggtc 92220ctgttgcttt ggctgaacac tgggtgttct agatgacaga ctggggtacc tgaggtttct 92280gatatacctc ccacctcaaa gcttctgtct gctttgttcc ttttcaacac ttggattgac 92340tccatccata ttcttaggat ttgggtcctc tcttctgggc aaaacagcct ggcaaattct 92400gggatctccc tagtttgaac tgaagtctct acacctcttt tcttagccaa acacagccat 92460tcaactccat taattcttag ctgattgccc actgtgttgt tcgtggatgc gagttgtttt 92520acaaacaata tagcaaatgt tggtcccttt gaaggaatgc tttctgatca gtgctttatt 92580tgtttgttag ttttttttaa taacttctga ctcaggagag ctctttccag aggtgtcgtc 92640tgagggactc tgctgtaggc catcaggctg ggggcagaga cggcttcgtg gatctaactc 92700cttaggtgcc tctcagcaca tcctctactt gaccttcttt tccacttgta catgaacgca 92760ggccctttga gaagacactg ggatctacct gtgtgcatta aggtctgttt ttctctctaa 92820tggctggagc tagatagagt cgaaggcaac ttgtgagcaa caatcaaact ctgggagcag 92880aagtgcaatg aaaagaaatg ggaagatggc ctgacatcac ctcagcccaa ctggcagaaa 92940accagtctta tgtatgaccc aagccaagag tgatgtgctt ctccaaacag aagggagact 93000ccacttcaac tacactcttc aggacacagc ctccccaaca ggtaacagga aagatgataa 93060atattaatga gatgacaaat ctcattaatc tactcttccc gggaagacag ccacctctcc 93120agatgccagt actaagaaga cctcgatgta tgtccagatt ggaatctgtc tctacctcac 93180ccagcctgtg tgcacatgag gagggcatgg gagagtcaga gaggctgtgg tgccatgcat 93240agggctggct caagaatcta gaccctcatt gcttactgaa ttttcataga tgttcttgaa 93300taactgtttt ctttctttca ctgctgcttg ctcacaggat cagtctgtag atcaattctc 93360atcagttcta catggcagct gataagacgt ctgcgtgact cctgccgaaa attgtccacc 93420atttcttctt tttgtttgtt ttagggcttt gtttggttgg ttgatttttt tttttttggt 93480ttggttttct ggtttttgtt ttgtttgttt gtttgttttg agatagggtt tctctgcgca 93540gccctggctg ttttgaacac tcccgtttgt tctttaacat gaaggcaaag ggaagcatat 93600gccaaagctc tctatgctga ctcactacac tcacaccctt cacccaaaac tggaggtcaa 93660acctagggac ttacaggggg ctggacttga aatatccata gcatgagccc tagtccatct 93720tcacaactgt agaccttatt ctagaggccc tgggactgag tccaagaatg actgggttac 93780ttgcctccag cagagggtct atggatgaac ctatccatcc ctgcactacg ccaggcctca 93840caaccacctc cccaggccca cattaacctt tgctgaaatg gaatccaggg tctcatgcat 93900gctaggcaag catattatca ctgaactccg cctccacccc tcagcacctc ccttcttgac 93960taagtgcctc ttctcttatc gtgtacacag acactttaga aaatcagtgc cacacgagac 94020agcttatcct tgtgcagtct cgttgtccgt ctgatttggg gtttgatttg atagtaacca 94080aacccaggcc ttggcacaag ttaggtaagc tattctgtca cagagctcct ccacaaatct 94140gtgtgaggtt ttagtaattt ctttgggtaa tatgtaattt cttctcttca actggatcat 94200taaaaagtct actatgggcc aaggagatag atggctcagt aggtaaaaga gcttgccctg 94260atggcctgat ggtcagagtt cagatccccc atgtgatagt ctccgtgtct ctacactgag 94320atgggagaca ggagaatcac ccacaaggtt atgggacagc tagcctggta tagacagtga 94380agtcgtagaa acaagaaaca cttggccaca aaacaaggtg gaaggcaaga agcaactcct 94440acaagttgta ctctgatctt cacacacgtg tcctggcatg caaactccta catgcacaca 94500gcaatattaa tcatgtcaat taacaatata ttcactcata taattatatg ctaggttgta 94560atagataatt gtgtgatatt ccctttttaa aaaataccaa aactgtgttc tgtgtactca 94620tgctacatat gcattaagct acatatacac aaagggttta tgaccaaaaa ggactgttat 94680ctttcctttt aagatctgtt ttcttgccag gcatggtggc acatgccttt aatcccagca 94740ctcaggaagc aaaggcaggt ggactgctat gaatttgagg tcagcctagt ctacagagtg 94800agttccaaga cagacagcca aggctacaca gagaagtcct gtcttgaaaa gaaatcaact 94860agaaaagggc agtgttccca aagcaggaca catgcactgt gcttgcttag agtgtcagga 94920acattaagat gagccagcct ctgttattct aaggatatca gtgcccctct gtgaacaaag 94980gccttactat gacttagcta acaggcagtt cgtggatcag atgctgtgct tcctttcagg 95040acaaagttca taaaaggtgt taggtaacaa aagttgccca ctggttttgt gtgcatttgt 95100gacccaatgt gaagcagtat cagtgaacca accgtgtgcc tgggtgtcag gcctccatgt 95160attccccagt ggcatcaggc aacttctctc tcccagtctc tggcattctt ctattctcca 95220ctctgtggac tcattaaacc aggaagatgt ctgtgctctc tatagcactg ctatactccg 95280gtacggactt cacagcctgc attctcctag tctaagatac acatagattt ccatgattgc 95340cttctattag atttcttcat ggaggaaaat cagtgtcttg atatggaacc attacctcca 95400aagttactga ttggcttgta attatcatat gtactaaaag cctacactat tgtcttgata 95460tacaatatta ttctgagatt ttaatgcact aatttctttg aaatgaagtg tttctttaaa 95520cactgtatag tgtaaaataa gcataaacac cgccgtttaa aggtaagagg aagaaacact 95580tggtcctcta ttaaaggggg gaaatagcag tgacaatgat acttttcctg taaaaagttg 95640atggtttttt tttttgttgt tttttttctt tttttgagac aggatctctc caggtagcaa 95700tagctgatct ggaactcact attgacatca ggctgattca gaattcacaa gatttctctg 95760cctctacatg cagagtgtta gaattaaagg tatgcatcac catgtctggc ctagcctaca 95820aattcaccca ccaccaccac atcacatagc tattaattaa aaaataatgt tcactttaaa 95880agtgggaagc cctcctgaga gtctatagga gtaatgccag ctaagaatcc tagcaactgg 95940agatataaag actgaagtag ccacctccta tagccagaca ggacatccag tggaagaaga 96000aggacatcaa tccacccaca aaaccttcca cccaaaatct gtctgcctac aagatgtgca 96060gggataaaga tggagcagag accgagggaa cagccaacca atgactggtc caagatgaga 96120cccatcccat gggagagagc caaccctgac gctattaatg atcctctgct gtgcttgcag 96180acaggagcct agcataactg tctcctgaga ggtttcatcc agcagtgcat tgagacaaac 96240gcagagaccc acagccaaac atcaggcaga gctttgggag ttctgtggaa gagtgcggga 96300taggattgag tgtgcgggag cagtcaagga caccacaaga agacctacag agtcaactaa 96360cctgagccca tggaagctaa cagagactga accactaaca aagaacatgc atgggccagc 96420ataggtgccc tacacattta tagcagacgt gcagctttgt cgtcatgtgg ctcccctaac 96480aactatgtca gggctatctc tgactgagct gcctgccatt ggaaccctcc ccttcctcta 96540gctgaactgc caggttgcac ctcaatggga gaggacacac acattcctgc ttctacttgg 96600tgtcccaggg cagggccgta tacaaggggg gcttcccctt aggagaagag gaagggataa 96660taggaagagg aacttatgag ggttagaagg gggctggggg gctgtgatca ggatataaaa 96720tgaatacata cattaatgag aaaagtgata ttatacttct aaagtgttgt aaattaatac 96780tggttctttt agaatacagg agtttaagtt tgccaatctg cctttcacct ctatttggcc 96840gtgttaattg ctgtcagcac agagaattcc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn 96900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 96960nnnnnnnnnn nnnctccatg tgctctaaag gcctctctac ctaactctct aactctctct 97020ctgcctctct gactctctct gcctctctga ctctctgcct ctctctctgc ctctctgcct 97080ctctgcccct ctgcctctct ctctgcctct ctgcctctct ctctctgcct ctctgcctct 97140ctctctgcct ctctgcctct ctgcccctct ctctgcctct ctctctgcct ctctgcctct 97200ctctctctgc ctctctctct ctgcctctct ctgcctctct tgcctctctt gcctctgctc 97260ctctgcccct ctgcccctct gcctctctct gcctctctgc ctctctgcct ctgcctctgc 97320ctcctctgcc tcctctgcct cctctggcct ctgcctctgc ctctaagtgc taggattaaa 97380ggagtgggcc accatgtcag actatgagcc agccttcaaa tcccagtcaa acctggcccc 97440ctctctgctc actgctataa acctctctcc tcagtcctac ccaaccaggg cctcagtctt 97500ccacaggagc gcttatattg taatgtgccc gactcttcct ggcacagtgc ctggcacaca 97560gaaaactcaa aattaactta gcatcaagag aagaaatgag atgtcctgtc acgttctgga 97620tgcagaaact gcagtcggac aaagatgctt ggtttcccca aggccactcc tgcccagtgc 97680cagaggcaag gccagagaat gatccagttg tgatccctgc tctggattct ctcactgacc 97740ccatagctac ctaccaccac taaatcatag tgctccatat tggtaacttt tgttattctt 97800ggttagttca ggccaagtct aaacgcactc tatagccctt taaactgtct tcctgtctcc 97860acctctcaag tacagggatt atagggtata gcaccacacc caacccagta cttttattaa 97920tcacagtatg taccacacac cccgaagaat gttggaatat agatgcaggc tgaaaagaac 97980accagccgat tacctgccta agccctgccc cgaagcctac ccccacccca agttaaccac 98040caccccctct atgttaagca ctgcctccct ctgtttcctg tgtgtatcat ttttctgagt 98100ctccctaaca ttgtatttct tgtcctctgc ttgtttggct agctgtgtgt ttgctactct 98160gtttccagaa ctgcacagaa tccaccatct cgcttaccct tcttgctcgc cttccctgtg 98220ctgtgaaccc agagcagctg cttactagaa aagccaacca caaaagcagg tggagccctc 98280ccatcagaga gcaccagccc tctcctgaaa gccactttat tttaggcacg gagttaatag 98340cgtacaaatg acatccacag taatgtggaa ttagcatatg tctttttttt tttttccagg 98400aaagaatttt gtatgtgttt gaaaagtcaa cttgttcagg cccttttctg acagggctgt 98460tttgattcca gttgtctgac agcaggtgat ctgaccttgt gtttggaggc agaaaagagg 98520ccccaggaga gttcaagaga aaacaggctc agaggactca caggcccctc cccctcttcc 98580ccccacccca cccccctcct gccagccctt actgaggtgg ctttggccca tagctgccct 98640agcccaggga cccagtggca gacagaagca gacctacttt ggtttccatg acaactgcag 98700gcatcaggag gcggaccaag tgagccgcag ccaacaaggc agctggtttg aagggtgttg 98760atctctctgc agaccagcaa ggcagaacct ctagggacca gcagcaacag aagccaaaaa 98820ggagcctcta aatctggacc tgcaaaatcc ttagggacct gtctacacag ctcagccact 98880cacctgccac catccttcac ctacccacct ggacaggcac accactgtgg atatatgctc 98940atacctaaag catagcagac ttgtacccag acccacctcc acctacacaa atacagcctg 99000ctacatctat tcacaccccg atgaaaagaa gcacagacca aaagatccag gcacacactc 99060aagatgcgca cacccattta cacaggtgac acgcccagga agttcacctt atcacgagga 99120cactctcaga tacatatacc cagattcaga acacaacctc taggtgccga cacacatgag 99180gcacatgaac ccacgctttc tgaaccaact cagaaacagc attgctgttc ccgaggtgcc 99240tcagctgaat gacagagtcc aacaaatgac atgcgggcac acataccctc cttctgcttc 99300aggagccaag tcccccatgg agtccggccc ttaatcctct ttgtttatta gtgtttgtat 99360gcacgcttcc tccagaaatg cttaaaggac aaaactctca gcatgctcct gttgttacaa 99420attaaaaata aacagtgtac tgaagcaggg tatggtggca catgcctttg atcctggcac 99480tgagaaggca ggggcaggtg gatcgtcagg agttcaaggc cagcctggtc tatctaaaaa 99540gaatactact aataaactct tgctaactgt ctcacagagc cacatagctc tttaaagcac 99600ccagtgcacc ccacactccc ataggcatgc tctacaatgc aaggtggggc ctgctgcttt 99660gcacacccaa atggagggca atgcccagag catggagtag actcctaggc cacaggtcac 99720ctcctgagcc agttataaca tctgacagac atagcctctg tccacagagg ccactgagag 99780cctagggtcc cagaagcctc ctggaaggac attttgttcc tttgagttct ctgaaaatcc 99840aaagacggct ggcaccatct cagtcttacc ccaactgtag cttgtgaatc aacatcaaaa 99900tgagaaagac tgtcctacac ccatgtgcct gtctgtctgt ccttacagca ccgttggtta 99960cagtcataat tgccaggcat gttggctgtg ttgtgtactc tcacactggt gatgaagata 100020atctagaact tagaattctc aaggacatca cagaaaacca caaccaatca aaatgcagag 100080ttggggagcc cattcaaaac agagacatct cgctgggcgt ggtggcacat gcctttaatc 100140ccagcactcg ggaggcagag gcaggcggat ttctgagttc gaggccagcc tggtctacaa 100200agtgagctcc aggacagcca gggctataca gagaaaccct gtctcggaaa aaaaaaaaaa 100260aaaaaaaaaa aaaaaaacag agacatctct aagacaactc ctgcacctta ggctccggga 100320tcactgcagg aaagggggcg ggaaaattgc taagagccag agcagcaggg ggtttgctgt 100380gagaccgggt cacctagaaa cgtcagaagc tacacccata aagactctcc aacacgctgc 100440ctagacatgg gttgaacaag gatgattgct accgcggagc gaggagaggc catgcagcct 100500caaccccatg cagctaagac cggccgctaa ggaatgaatg ccaagaacag gagaaaggct 100560tcctggggga gagcacactg gttatccagc accaaacggt cagccctata aacacccatc 100620cgagtatcat tatacaggct ggatggattt cattcatgta ttttggaata cacacataca 100680gttacataac aaagatgaaa aaagaggtca tgaatttgaa agagagcgag gagaggcata 100740tgggagtggg gtggagagaa gaaagggaag agggaaataa tataatctca aaaaaagaat 100800gtgtgtgtgt gtgtgtgtgt gtgtgtgctt tttattctca gagttgttga gatagacaat 100860cgattccaca

ggttataatc tgatagctac tttttggttt tctcagtaat agtgactgtg 100920gagtgcctga cagcacagtt tcatgtacac ctcatttgtg tccctgagta ggatgcagaa 100980ggaggagaca gcgcagagct tagcaagacc cgggcctgag ggtttggaaa gtagctttgg 101040aacttggaaa gatgtcagcc caggaaaggg agagaggagg cttcagggag gaagccactg 101100atgacgtagg cctgaaagca caagtcagca tagtcaaata cagagctgga aagggccctg 101160atgcccacgg agccagagca gagggtgtgg gcctgtgtag ctaggctcct ggagagagcg 101220agcgggcagg taagagcaac agtaaactga gatctcccag actcgggtct ccagcctcaa 101280gcaaaggatt ttgtcgctag tgaatcacca gctgtgggaa tgggtgatag ctgtgccatt 101340gggctggtag tatagctccg gaggaagagt gcttgactaa gccctggctt ccatccccag 101400tccctccaaa atttgtcatg gaggagcagg gctataaccc tcagctatca gagaggtgga 101460ggcaggagga tcagaagttc aaggtcttct ttagatacaa agtgagttta aagccagcct 101520gggctacatt ataccctatc tcaaaaccaa acaaccaatt aaggagagaa aggacagaag 101580ataatgaaaa tatctcaagt agccagacag tagacatcga agcggggcca cctagggact 101640tcatgtgtcc acgggggcag tagattttgc ccagtcatga acagagccag agtaccggtg 101700acaccccaca tctagctcag agcctgaccc accaaaggct ttacataaat gttttctgaa 101760ctgcgtggcg ccacggggtc acgcgaggaa aatggaatta agagtcgagt gacccgcagg 101820gctagttcag gcctggtatt gactggttcc tggacctcgg ccggagattt aacctctcct 101880gttctcgatt tccacgctgg taaagcgaat ccgataatgc cctgacctac atggcttagc 101940tgctgcgggg ctgcagcagg atgcgggact cggcctggct ggagccatgc tggccgatcc 102000ctccagcgct tgggactcac ctcgctccgg gaccagatgc aaggagagct ggctcagccg 102060tcagcaatta cctgcgcccc cacccaacac ctgcgctcca ctttggagag ggcagatggt 102120gcaactaact tctcagtacc gccccactcc cggcgccagc ctagaccaaa cgcagagcca 102180gcgcgggcca gcgtgtagcc agttcaggac tccagcacca gagccccttc cgctcagcct 102240cagcacagta cctccagctt cagctcctca ccagccctct aaactgtgaa cagggaagag 102300agctgggccc ctgcttttct tataaactga ggatccgaga aatgaggtgg tttgcagaag 102360gaggcaccag acagaagccc aagtctctcc aagtccgctg ctacgttatc tggagctgca 102420ataattccaa ggactactct ggcaaagggg cctaagtgtt ccttggggac aggggcatgt 102480ttgctatatc tgtatatctg tgtacgcgcg catgtgctca catacacaca cacactcaac 102540ttgtgctcca agtaattcaa ggttagtgga gatctaagtc aagcctaaac caggattcaa 102600tgattatatt tttaaaaaca ttaaaagagg cctggcatga tggctcagtg ggtgcttcct 102660taaccagaag atctgagttg gattccccag aactcacaca gtggaagaag agaaccaact 102720tttgcaaatt gtcctctggc agacatggtc tataaccccc acactcagac tgagtcagga 102780ggattgctgt gagttcaagg tcagcctagg gtacttattg aaactttgac aaaagagaga 102840gagagagaga gagagagaga gaggaaggaa ggaaagaaag aaagaaagaa agaaagaaag 102900aaagaaagag aagaaagaaa aggaaggaag gaaggaagga aggaaagaaa ggaaagaaag 102960aaagaaagaa agaaagaaag aaagaaagaa agggagaaag aaagaagaaa gaaaaggaag 103020gaaggaagga aggaaagaaa aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaag 103080aaaggaaagg aagaaaagga agaaaaggaa gaaagaaaga aaggaagaaa gaaagaaaga 103140aagaaagaaa gaaagaaaga aagaaagaaa gagaaaatga gtgaaataaa ccagggagct 103200gtcccaccat ggtggtgcac ttagaaggca gaagcaggca gatcttatga gttccaggcc 103260agcttggtct acataattag tccctggaca gccagggcta tgtacataga ccctgtctct 103320aaagaaacaa agctagagag atacatagtc aacaaaagcc ctgcctcgtg agaaagagaa 103380cctgagtttg actcccagaa gtcacacagg gcttggcact tttctaatgc cagcactcag 103440aaggacagat taaggtggat ccctgaggtc cactcactag ccagccgcca tagcctaatt 103500ggtgaggtcc aaaaagcaag gtagtgaggc tggagaggta gctaagagta cttacagctc 103560ttgtcgagac tcagagttca gttcaagcac ccataccagg tggctaacca cacctgtaac 103620tccagttccc agggaccctc cgccccttga gcacctacaa acatgcaggc acacacacat 103680acaaataaat aataagcgaa tcagaacata cacaaaatga catccaatgc taatacctga 103740ctcctcttgt acacacacac acacacacac acacacacac acacacacac acaccacaga 103800gagaatggtg agccgactct actactcttg gaactgttgg ttctcctgct tagaagggat 103860catgaaacaa ctctaaaccc gctccggtga gtttactgtg ccctctttgg ctctcattac 103920ctgagaccta tctgcctcct taacgatcca gttgaagcta gagagggttc agtggctaaa 103980aggacttgtt gcacaaacac gaggacctga gtttagatct catcacccac ccataacaag 104040ccaggcttgg tctcctgcct gcatacctgt catcccaaac tgtgaaggtg gtaaggcgga 104100cacaggagaa tccctgggca tgatgtcagc cagactgact gaaaactttg agctctgggt 104160ttgaggagaa accctgcctc aaaggagcaa agcagagtgc tagaggacag ttggcaccca 104220cctttgacct gtgtgtgtac ctgtgtatac gtgtgtgagt ataccacaaa cacaatacac 104280acactaataa acatatctat atataaacaa gaaatgcaaa gatacagttg tctctattgg 104340aactacctag agcaaatcaa atgtcatcag tttccaactg gatccttttc acctcacaga 104400accaactaca agaccaagca tcgatagagg agctagagaa aatacccaag gagctaaagg 104460gaactgcaac cctataggtg gaacaacaat atgaactaac cagtaccccg gagctcttgt 104520ctctagctgc atatgtatca aaagatggcc tagtcggcca tcactggaaa aagaggccca 104580ttggtcatgc aaactttata tgccccagta caggggaaca ccagggccaa aaagtgggag 104640tgggtgggta ggggagtggg gggaggggta tgggggactt ttgggatagc attggaaatg 104700taaatgagga aaatacctaa ttaaaaaaat tatcataaaa aaaaaagacc aagcatgaga 104760ccaaaggaga ctaactcttg gtcaagagca ggagaagcag gcaatatgct ctaaaatcaa 104820ctgctctaga tgctggggat ccagaagtta tagctccagg taagacaaaa tgagggtgag 104880caacaggctg gtaatggagg gaattgtgtg tgtcttctgg gagtggatag agctttccca 104940ggaattctgg ggccacctcc tttggctcct tggatggttc tttctagttg tcatcacagc 105000aattgtcaac tgtcaagaca ctggagtatg tcgtttagtg tggggatgac attataatta 105060agttcacagt tatctggcag ccatcttaga tggcataggg agagacactt ctgcatgcag 105120gcatcttgtc ctcagagact gtggtggcta atcttgattg tctactcgat gggctttaga 105180atcaccatgg aaacaaatct ctggtcacat cagtgaggga ttatctacat taggttaagg 105240gagttggaag acccagccta agtgtgggca acaccattcc ttccatgggc tggggtcact 105300ccatggcctg aggtccttcc atgggctggg gtcattccat gggctggggt cattccatgg 105360gctggggtcc ttccattggc tggggtcctc ccatggctgg ggtcctccca tgggctggag 105420tcattccatg ggctggggtc cttccatggg ctggggtcct tccatggctg gggtccttcc 105480atggctgggg tccttccatg ggctggggtc cttccatggg ctggggtcct tccatggctg 105540gggtccttcc atgggctgag gtcattccat gggctggggt cattccatgg gctggggtcc 105600ttccatgggc tggggtcctc ccatgggctg gggtcctccc atggactgga gtcattccat 105660gggctggggt ccttccatgg ctggggtcct tccatggctg gggtccttcc atggctgggg 105720tccttccatg gctggggtcc ttccatgggc tggggtcctt ccatggctgg ggtccttcca 105780tgggctgggg ccattctgtg atatgctttc tgggattgaa taaaaaggag aacatgagtg 105840agcacatgct ttcatctctt tctacttctt aactagagac acagcgtgaa cgttggcttc 105900ttctgtccgt gctggtgtga ctttcccact agggaaggac tgtctcccct agcaccctga 105960gctaaaacac acccttcctt tcctaagttg cgtttgtgca gtatttttgt agccgtcaca 106020atcaactgat cccatgaccg agcaaggaga aaagcagcta ggctgtgtag gtcaactctt 106080ctcacagccc agtctgctga agagggtaac ttactctctg acggaatggt taagctcctc 106140ccccacccgc cagaagtcct gctgtgagct cctctccctc caccttgagt ttcagagttc 106200agtgcaaatt tgagttctta gtttgtgtca aggagtgtgc taagacgctc cctcgacatc 106260tcatcgctca ccccaggagg tggctcctca gtacccccca tttgacagat gaagctgggg 106320agcaaaggac ctgggacaaa gagacttagc tcctctctgc taccctgcct gggctctcga 106380ggagagagaa ggattcgtgc gctctctccc agctgctcca gcagaagggc tggaactagt 106440gccgctgttc ttagagtcca aaccgctggc tttgcaccca ctcccacatg ttacgtacga 106500tggcctgggc aagccacctg tcttgtctcc tgaccgtaaa agagagggca acggtgtcgc 106560ccagcttctc tgtcataacg ggggtgaact gagatgcact tgtctaagga cagaaagatt 106620gcttagtagt tgagtggttg ctgctcttcc agaggacatg agttcaattc cctataacca 106680catgcccctc ccccaccccc accctcacct ccacccccgc atgaaaatgc ctgtaactcc 106740cgcctcaaag aatcggatgc cctcttctgg cctccgttag aaactgcatg catattgtga 106800acccacaccg atacgcagta cacattaatt aaaagtaaat taagtctttt aaaaaggaga 106860gatttgtcag ggtgaccctg agcccgatga cccccacgtt aatccactgc agccggagcc 106920caggaaagtc gagcaaacga ggtgattcct gccacagctg ggagggggct gagtctctcc 106980agattcatga gctaagttca agtcctcaga tctcccatca ggcctggagt cactctgtaa 107040aatgatttgt gaagtgacaa gcaaaaatat attaccaatt taagagtgtg atttgcctgc 107100aactttttaa tcctccggct gttgttgaat agattaaagt gtctgctttg atccatgcat 107160ttagatggag acataaatat agccatcact tattttccca gccgcaggga tgggtgtcgt 107220gctgtgtctc acaggcttcc gaccccgcca gggtacggca agagaatgac ctttttgcca 107280ttcaaatctc tcttgcattt cagccctgac ttttcagctc ctgaccgggc ctatcagcag 107340cctcctaatt cgatcactgc tcactccagg aaacagtcac caggaaggag gtccggcgca 107400ggcgggaagc agcggccgcg cagtgccctg gtgctgtgaa cttcccctgg ctcgccagag 107460ccctgcaaaa ataaatctcc ggagaaatag attttgaaga ggaattcggc acatagatct 107520tctgtcagcg gagcagactc tgcctcagcc gtttttcagg ctcgctgagt atccagaaat 107580acatctattt ttacatggct ctctagcgag agttcctctt gcctggaggg gtaagaggcc 107640gtggaaggag tcagaggatg ggagaggaga ggggtgcggg cactgactgg agaaggacag 107700ggaggtgacc gtgcagtgct ggtcttcccg gttttcctgc atcctgcagg accttgggtt 107760tgccaaccaa gtttgggttt gcctcgtcgc tatgaaggct ctgtgtgtcc cttggcccac 107820cggacccatc ttagtaacag gcctgatgtc cccggagaag acaatacact acggcagttt 107880ggcgcgcgcg cgcacaaaga gctgcagcac cgcccggggc tcactgtttt gttctcggcc 107940tagctgtcgc ggtgaacagc aggagccatg gaccagatgg ctggagggag gtgcgagcgg 108000atcgcactgg caggcaatta ggcctcaaac aatggcccgt gaaatgtcag aagtggcaag 108060gaagggtcgt ggtggaacag cgggatgaca acgtaaggat ggcgccagga accccggaga 108120cagttcctgc ttgctcccac aggcccctct ggcagaggga acactcaatg acccttctcc 108180ccaatgccca cgcagactcc tctgaagata aagaaaaggc tgaggggaag ctatagtgca 108240gatggtgagc cccaggtgta gatcgggtca gtagatgctg gcaccaaata aaacctgtca 108300tgacgataca cacatgcgat cccagcactt aaagaggcgg gaggaggatc aggagttcaa 108360gatcatccat ggccacatgt atagtgagtt cgaaggcagc cttagatata ggtgacttca 108420aaagggggcg tgggagagag cgaaagaacc gggggggggg gggattgaaa gagaaacagg 108480agaaggaaac agtgctggag aaggagcaat gagagaagac agggaaggga ggtgaggaga 108540gagagtgtgg agaggagagg agtaggaacc gcccctcccg gtggctgcca gcaaccacga 108600gaaccccaca cccactagat gctggggtta aagtgaggct aagagatggc gacacccacc 108660cccttggcca ccacaactaa gaattatgta gagtaattaa tcaagagcta atgagaaatg 108720tgttttctct cgcagcctag ccccacatca tattcttatt agcggaggtt attacaatag 108780taatcagcgt gctccaggcg caaattgggt tttttgctcc actgccacca atttagaaaa 108840ccattaatgg caagataagg cccccgtggc tcacaccggg ccaagcctgg gaacctgcag 108900ccaactctct gctcatgaac ctacacgccc atgctccagg gggcgctcga gcaagcatcc 108960tggctcttct gggacgacag agaagaaaaa gccaaccgct ttctaggagg tgctgtacct 109020gcgggaccat agaactttct caaatgtgaa tcctatacaa cagtcacttg gatgagacaa 109080cctcatggaa caaaacatta aagccaagtt tgccactaga gccagagcgc catatactgt 109140gcccttgagt cctgcttcaa gccagtctgc tagaaacgct taggtgaatc tatagctctt 109200aggcaggagg ttgcgatcag acacaagccc tggaacaagt ttctatcagt gaaaagagtg 109260gagagttaag agtttggggt ttttcttata ataataataa taataataat aataataata 109320atagccgggc gtggtagtgc acacctttaa tcccagcact tgggaggcag aggcaggcga 109380atttctgagt tcgaggccag cctggtctac agagtgagtt ccaggacagc caggactata 109440cagagaaacc ctgtctcgaa aaaccaaaac caaaacaaat aataataata ataaaaaaac 109500ttgttattta cttatttgtg tgtataccac agcactcgca tgaagatcag aggacttctc 109560tccttccacc atgaggggcc aagagattga actcacgttg tcagggtttg gaggcaagaa 109620ccgtcatgtt tggcagcaaa gacctctacc tactgagccg tcaccatagc cctgctgaga 109680tagtttcata acgataaatg cactctactt tttgatgatg ttctcagttc caaaattgtc 109740tccctgtgtg cgtgtgtgtg cgcatgtgtg tgcgcatgtg tgtgcgtatg tgtgtgtgtg 109800tgtgtgtatg tgtacttact catatgtctt tttatgtaca aaagataggt tccttcatgt 109860atagttggag aagggctggg tgatctgact gggaggaaaa gaaagaaaac caggagtcac 109920gtctcttctg cccccttgtg ggatcctgga tcaaactcag gttgtcagga ctgcatggca 109980gggtctactt gctgagctgc ctcaccagtc ccctttcctt tttcaacatg tagaggccaa 110040gtatgtccct ggttctacac agcagacaaa gagttaagaa gcaacagcta tactgcagtt 110100aagtcctgcc ttttgtttgt ttggttggtt ggtttggttt tggtttgaga cagcctttct 110160ctctgtagcc ctggctatcc tgaaactcac tctgtagacc aggctggctt tgaactcaca 110220gagatccagc tgccactgcc tcctgagtgc tgggattaaa gatgtgcacc atcatcaccc 110280agcttagtca tttctaccgg tcactattat ttagttagtg ttacttagtt ccttgaatat 110340gttgtccaca aaaattctct caaataatta actacttcat cctgtggaat aaaagatttg 110400agtgaggcag acctaagttt gaagtctgtc tacttatcac tggcttttgg tttgggattt 110460tatttatata cgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgca 110520tatgtgtata tgtgtatgca cacatgcatg caggtaccca ctggagccag aagagggtgt 110580cagggcccct ggaattacaa gtagttgtga gccacacaat gtgggtgcta gatgtggctg 110640ctagacatgt aagctagaca tgggatctag gaataaaatt cggaaataac aagcactcaa 110700ccactgagct atccctccaa cctgtctgtc tagttttgag aaaagcatta gcctgctaaa 110760gcctccattt cttcatctat agaatgggac tgtacctcca tgttctttga aagttccaaa 110820gacatatccc tgtgtgcttt ggtaaataca gagcacctgg aagtctcagg cactatttga 110880gatgcaatat atggagaaag gactcatctg gggatctccc taaggatgcc cgagagacca 110940gcaggtatga tacaaaagag aattagccat ttgacataag agagacatga agatcataca 111000ggtgcatgcc aggcaagagg gatcctgagc ctagaggttg cctgtcagtg ctatgagcat 111060ttaggaaggg ggctacctcc agacaagctt gggccaactg gtggaggaga tggcccaagt 111120ctgagaagac atatgggatg aaggcggggg ttggggtgag ggcgtctcct gcatggagtg 111180gtccaaacag aatcctggag atgtgaacag tgaggctagt ggcctctctg gatctaagga 111240acacacagca gccaggcctt cccatctgag ccatctgcct tttcctcctc cgccatagct 111300ccaccccctc tgttctgata tcatccgggc acagccagag gatggcacag cagaggggac 111360accttgggcc taagacgtcc ttagcaagag cacctacata cccacataat gccccctcct 111420ccatgcctcc ttaacaggac taaggttcaa tgctgctaat taacaaacag caatcaactc 111480agctaccccc ccccacccct tcaccagacc ccgacccccc cgcccccatg cttcctttcc 111540ctcctgtgga gaaggcacag ctgagctgca ggcaggggca gagggtggga gcaggctgag 111600gtagacacta gagatccggc agaggggtta cactcaatat tgagttgtaa attcaacaaa 111660ctggagatct ctctaacgcc catctagatg agcatctggg ataacagaca tttctagcta 111720gacggagctt ttgcctgtca catggagtgc cattagttta gtcctcagac agtccatcac 111780aaccctcatt taacaatgat ttaacagtgt ccttggcccg ctctaacact aaggaaggca 111840ctgtcatgta tgctcaggag gtcacacagg gcatagaggt gacattcaac caatagacag 111900ggtgggtgga aatatagatg catataggtc aacaggcagg tgaagggaca aaggtggtgg 111960tgggttagcc cattttacag aacaagaaac tgacataagc caggaatgat gactgacacc 112020tgtactcccc acacttgggt ggctgaggcc agaggattaa tgtgggtttg aggtcaacct 112080aggctccaga gagggtctca agtaaaaagt taaaatgtaa aggaaaaaag aaagggaagg 112140agggagggaa gaagggagga aaagagagga aataaaaaga gaaaagaaag tcgggcagtg 112200gtgatgatac acactttttt aatggtgcac ttgggaaaca gaggtgggtg ggtctctgag 112260ttcaaggtca gcctggacta cagacagcta gggctacaca gagaaccctg gaaaggagaa 112320gagaggagag gggaggggag gggagggaga aagagagaga gagagagaga gagagagaga 112380gagagagaga gagagagaga gagagagaga gagaagaaac tgagacaaag agaaaacatg 112440acttactcta aactagacat ctagaggcag gtcagaagca ggtctcacca tccacccttc 112500tgttctctgc attcccagaa gccagccccc acctctccaa tctcaggctg gctgccatga 112560cacccgtctc tctgaaggct gagatgatga ggggaacccc agactccctg tgttccccct 112620cctctggaac ccaaggcctc acataggggg cacagtatga aggtccttga gtttacagat 112680ctccagagaa gccaggagtg ttaatcacac aggactgtct ttccaattgg aaacctgttc 112740gttcttccat ctccaaagct gagacctgga agtgatgaac agtctggtga tctgtgttgt 112800tatctgttgg accaggccat accaagctcc tacaaccaaa gctaatctaa atgaccctca 112860tagccagagg ctgccccaac caggaccaga ggtagctaat aatctaaatg acctctatgc 112920tatctgtgtg accaaaacca gaccagaccc ttaggccctt aagctagcac tggtaactca 112980tgtgacatcc ctgccttacc tctcacctgt cccacttcct aagggcccag catgcctccc 113040gcactcctgc tcctgtccct gaaccatgac agcctcacac caagttgagg tccctttgct 113100ttcagtttgt ggcatcacct cttctcgact aagatctcat gggtctgggc aagcattggc 113160tccccctctg cccagaatcc tttctttccc tctggtcttt ctaaagcttc aggtttctcg 113220gctcagaatc cttttatctc cagtctaaaa gtcctaagtg ggccttccac acagtttggg 113280ggtttcaggg acttgtgtgc atttgataaa aaaaaaaata gcccatggct agaggtattc 113340ttgccccccc ccataaagct tggagccccc ataccacttt ccacccagga tcatgtggcc 113400tttgccacag aagtctcctc agcaccacag actttgagtt cccttcccct gggcttggca 113460tgataccaga accagtcctc tttccagcct gtctcctctt aaacaaagtc agagcctgag 113520gccatcaagt ctttgcaggt attcacaatg acctctggac actacacaca acagatgtta 113580tcaagctaag gctttggatt cagctggcca gtcaacctaa cctcctttat accttctgtg 113640tacccaacat gggagacaga aaggtagaca aggtagagtc ctctgaagta ctagggttct 113700tcccgttaca taggagagac cactgaaatc tatggttaac tcctgtctgt aatttaacaa 113760ttaattggtc agtcaaggaa aagacaaaga taaaactcaa gggccttagc ttcttgggtt 113820ggttcaaggg tttgcaatgt gtgcttcacc ttgtgatgtc atcagctccc tttcagatca 113880gcctcttcct gatggggcca aatggcagag agagagctgc caaccggcag gcacatacct 113940atggtaccaa gtatctgctt ttctcaaggt gcaaacaggg ctcctggcag aagaaggggg 114000agggaagcca ggaagctaca gcctgtgcat gcaaatgaga cagctgcata attaattaac 114060acccaacagt ttcattagga gacagagctt tgcactgact gtcttggttc agagaatagg 114120atctagcact gttcaaagcc aaagtccgaa gctagagatg ctgcccacca ccgatgctgg 114180ggacaatact tgttgctagg agaggttgcc tggggcagct ttggtcctat cttagacttc 114240gtaccaggga ggatgagcct atgattgtca cataagtccc ctctgctcag gcctcaccca 114300cacttaggtg tgggaaggga ggtgggcacc tcagagagcg gaaggggatg cccctggagc 114360atctgtctga gctgtggctt cccccgaggg tcagtctttt ctacagctct gagaacagca 114420gccttgtttc cagaatggtc tctaagcgct gtaggatact gtacggaatc caaagccaaa 114480ccaggacagc taaatctcca gctcccttgt ccctccctca acaactaatg tttctctata 114540aaactgaggg aggaaggtag aggaataggc agctccttgc taaaccttct gggagcctct 114600gtccccagcc ctaatcccat cctagcttgc atttctgttg ctgtgataaa cactgacaaa 114660aaacaatttg ggggaaaggg gagtttgggc ttaaacttgt atttcacagt ctatcattca 114720agaacatgag ggcatgagtt caaagcaggc acataaaggc aggagctgaa agagaggcca 114780tggaagaaca ctctcaacag ctagctccca tgacctggtc tgtttgcttt cttgtacacc 114840ccaagaccac ctgccaaggg gtggcactgc ccctgtgggc tggaccccct cccagcagtc 114900actaatcaag aaaacatccc cctatccccc acagccttgc atacaggcat tctggtggag 114960gcattttctc catcgaggtt ccctcttccc agatgaccct agcttgtgtc aagttgggaa 115020gaaacagaac aaaaacttaa gcagctaagt ttcatggaca agaaacatca tctctcagaa 115080ggaatcctag caaacctcat gacctgcctc cgctttcctt tctggaacca tctctgctcg 115140ccacatacac cctgggttgt atccacatca gactacctcc attttccacc cccaccccca 115200tgagtggcct ctcatgtctc tgctactcct tcagttatga aggccctctt gcccttctca 115260cctacctgca gactcctctc ctcaggagag ccttccttga cgcacaccct ccctactcct 115320ccacccccta caaagctagc tattttcttc cctcttttct gcataacata tgcaataatt 115380attaatttac ttgattgact ctgaccataa gggagtcacc ccttgagggt agagactgta 115440tcttccctgt ctctgcatca ttaagtcctg ggctacactt caaaagtttg ccagatgagt 115500aagtgaataa atctgttaag ttgaaaacac ctgttataga tccttgactc tttatatttc 115560aaccattcag aagtttattc cctcgctaaa cctatagccc tgcactggag aaagcgtagg 115620gatgggaaag ggaccctcaa ggggactgag gtcatcagga agcaacaaca gtggctggtc 115680ccggcaggat aagagcctag gttggatagc ataaagctct ccattgttcc ccatagctaa 115740agcagctaaa agaaccccag gggccttcca acctctttgg ccatcagtct tccgcatgtc 115800ctcacatccc aacttccagc aatttctccc tccagcccga tccaggcaca caacagacat 115860agctctgcca gaatcacaga gccctcctcc aaagctcctg gcccttgttc tccagttttc 115920ctggggtggc

cagagaagac ccatcctcag tgccatggtt tgaatatgct tggcccagga 115980agtggcacta tttggaggtg tggccttgtt ggaatagatg tatcactgtg ggcatgggct 116040ttaagacctt catcctagct gcctggaagt cagtattctg tgggagctaa aaaagggggc 116100cctggggatg aagagggggg agatagaccc atgccccacc agagttttac ctattctctg 116160gtttgtcagg tgtgggaggg ctgctacctt tccactcatc cctggatggg catccaagcc 116220tctgaccctc tcttcagggg acggccaggg gcagccctac ctggggaccc tggagctact 116280ttgctaaagc caccagggtt ataggagaga gggatgaggg aagatgttcc caatacctac 116340gagagtgcat gcagccttga tgagcagaga ctctctatgg tttaagagct ttattataga 116400aatgcaggga gaaagagaga agagagagag agagactaga gagaaagaga gtaagaggga 116460gagaagagag gagaggagaa gagaggagaa aacaaagaga gagaagagag gagagaagac 116520aaagagaggg gagagaaggt gagagtgagg ggtaagaggg agacaagtaa gagagtaagc 116580agtaagagag cgaggtgggg ctgatcagcc ctttttatgg tctttactgt tgctaggtaa 116640ctggggagga gttcagcctg aaggtcagaa gcttggacca ttgcctacgt gactactgac 116700catgcttctc ttatgggggc atgtgggggg cttacgcagg ggccagagtt ccaggagcat 116760aagggaatgc ctaccgtgtc atgaaggtga attatgactt cggggttcag acctcagctc 116820gactggagac aagcctgcaa ttccccacag tattctgcta gcagccttaa attgtagatg 116880ttgaactctc agctcctcct gcaccatgcc tgcctggatg ctgtcatgtt ctcaccttga 116940tgataatgga ctgaacctct gaacctgtaa gccagcccca cttaaatgtt gtccttataa 117000gagttgtctt ggtcatggta tctgttcaca gcagtagaat cctaagacac tcagttaaca 117060aggatattcg cagttgatta tctgaggttt ctctgtaatt ggaaaagaaa gagcaacagg 117120caaatgtcaa ttttggcttc cctgcctccc atccctgagg gatggaggga acaagagtcc 117180tgtctactct caaggccttg ctccaaccag gtcttcttgc tcagcttttc tccataggct 117240catactatac acacacacac acccccctca agggtgatat ttgggactta gccagcccca 117300tggagcaagc ggcccacaag tccacagaca ctacagagag gttcagggac agtgagagag 117360aaatgtattt ggtgtttgaa tgtgtgcaaa gggagggaca gcacccagac cccacaccac 117420ctcgtctatg gagtcttatc ttcactgtgg tgttattcca cagctaagca cagccaacat 117480gcatcagctt gtgaatttct agtcccaagt tcactgcccc cagagagact ttgtgatcag 117540ggcctacaac tcttcttccc ttcacttccc tgggcccctc cctgaggagt tgaagcagag 117600acagtgtgga aagcaagacc ctacctttag ccgccattct aatccagtgt ctaccactcc 117660ttgggggaaa gatagcaggt gtcacaaggt ggtcacctct cctttccacc agaatgccac 117720ctcctgggtg gaagtccaat gttaccttcc accctcacat acacatcact gagatcctgc 117780cttctttcct gccacctctc cttcggtggt cagagatcat gtctttcttg cagatggcta 117840tgacagcctc cagataggaa ccagtgggga ccccaccccc aactttgtct tcacaatgag 117900gttgatgtga gcttgggggg gggggttcga gacagggttt ctctgtgtag tcctggctgt 117960cctggaactc actctgtaga ccaggctggc ctcaaactca gaaatctgct tgcctctgct 118020tcccaagtgc tgggattaaa ggcatgcacc accacatctg gcttgatatg agctttctaa 118080gccaattcca atcatgcctt cccccaacat taaatttttt tttctaccca ggtcctcaat 118140ataggactca aatctcccag aacaaatcat caacccctcc actccccgat gtggccttcc 118200cccagtccct tgaccatctc tgaacttctt ttattctctc accccagggc cttagctcat 118260gccattcctt catctctttt cctgtgttct caaggtcctg gacaccatgt accaactgca 118320cctctagcac tccatacaga gaaacgaggt ctcgcctagc aaattgatgg ctgaaaatga 118380ggcaaaggat gggtgggaaa gtcccatgga attgtagacg cttccccatc ttattcattt 118440acctgtggca cttccattca aacccttgct gtacacaggc tgggcaaacc actgggctat 118500ccccagccca actcttgtaa aaccgaacag atccagagag gtgaggcaaa ctcagccaag 118560gccacacaaa gacttgtacc acatcccatc acccttggtt gtctcagaag agcccagaaa 118620gaccctagcc agaagccgag gttggactga gaccctcagg atgagttcag ccagcagggg 118680gcaggagtaa gccatggcca gtactaggct ctcgagaagg aaaactctgg ctaggaggca 118740gaagcaggca gatttctgag tttgaggcca gcttacacct cccactgctc caagtctaac 118800cttgggcttc ataacttcct gtggcctctc tggacaccaa gagccatttc cctaaggccc 118860aggatttata gaggacagca aagatagcca gccactcaac cagccccagc cccatctgcc 118920agagaccagg catccagacc ctggtagggg gaagctgtgg ctggtaaata aaaaaattta 118980ttaagaatat gttagctgag ccgggcagtg gtggtgcacg tctttaatcc cagtacttgg 119040gaggcagagg caggcggatt tctgagttca aggccagcct ggtctacaga gtgagttcca 119100agtcagccag ggctacacag agaaaccctg tctcgaaaaa agaaagaaag aaagaaagaa 119160agaaagaaag aaagaaagaa agaaagaaag aaagaaagga aggaaggaag gaaggaagga 119220aggaaggaag gaaggaagaa aggaaggaga gagagagaga gagagagaga gagagagaga 119280gagagagaaa gaaagaaaga aagaaagaaa gaaagagaaa gaaagaaaaa gaaagaaaag 119340aaaagaaaag aaagaaaaga gaatatgtca gctggagtga agcaatggct cggtctgtaa 119400agaatttgcc ttataaacac aaggacctga gttcaattcc cagaatccag taaaaaacgc 119460cctgttccta agccagtgta agaggcaaag aaaggagggc ccttgggcct ggctggccag 119520tctgtctagt ttaattggtg ggttccaggc caatgagaga tgctgtctca aaggaggtgg 119580acaagttctc tgaggtgaca ccaagactgt cctccagcct gacacacagg aaagcagcag 119640gcttgcttgc acagcgcctg cctgcccttc atggtggcca gccttccttc ctcttcctgc 119700tcactctagt gaagacccca gggtagtgag gagaagctgg agaggaaaca aggaaccagc 119760agacagctgg gggtgctggc cacacactcc ccaaccctgg cagcacaagc cccatcattg 119820ggtgctaatt aaatccccct aatgaggaaa acggatgcag gagctggctc ctccgtggcc 119880tccccggccc accgcccttg cctctccatc ggacttatcg ctcgtcacag gatgtttaca 119940ataattttta atttgtgcaa ttattaatca cttttctatc tccctcattt gcataataat 120000tagtcatctc actgtttggc atgcctgtga atgacagtgt tttaaattga attaagattt 120060taatattcta ttttcctgct ttcttcccct ccacacgcta atcaaagtca gatttggcag 120120ccagagggag ggtacggagg aggttggcag gcactggact tggcccattc tgtgctcctg 120180agaaccaacc gtgctgagaa cagggcctgt ggagaaggca acaagcaatc accctactcc 120240aaacggaggc tgaaggggtt aacccagcca tagctaattg agcagaggta atgaggagtg 120300gggaggggca gccagggagg aggggtgggg agggaggagg gtcacaggct cagcggcacc 120360agagagcaag aaaagtgctt ttcatttatt ttcttttcca agtaggctgc ttttggtaag 120420ggaacactag aggggacatg ataggccagc cattgatccc cagttagact aggttaactg 120480tgaaggctct gagtcccgca agtggaggaa gcaggctgga gcagcctaaa atcccattca 120540ttgcgtctct ctcaacgtgc aagacaattt tctggggttg actccctgtt cttctcccca 120600cttctccaca caccaacagg tcctctgccc aagttgggtg cctcacgggt gaggaacaag 120660aagtaaggga tttccaccga caagggacag cagacaggcc acgtgtcccc taagttagag 120720gcagctgtgg ctctacagat cattttctaa ccctaaatcc ttcttctaat cacgccaggg 120780attcagcctc agcctggagg agggcctgtt ctattgctgc ctccctttgg attgcctttc 120840gtgttgccat ggtgatccct aggctaccca ggatacccat tgctgcccca gaattctctg 120900cttgagcaga gcggctctcc cttcagcctg tttagaacac ctttcttttt actatgatat 120960gctcaagctc tgcaaaaacc aacaccgctt tgtccataca ggtgagtcgg gatggacatt 121020ggctcccaga gatgggctct gtgcaaacaa tatgaaggca ggtcagccat cacaggcaag 121080gggctggaaa ccacactcca acacagccac caagctcttc ccccacacca cacaagggct 121140ttcaactctg cacatgagga ccaaaatcag gaaagtcccc catgagggaa agaagaaaag 121200gggtcttgtc aggaggagag ctaggctgga agggtttgaa cccctacctc cagtgggtcg 121260cctccttctc tggatcccct ttggctcagg acatcccaga gcgtccttgc cctcctacta 121320attcctgctg acacacatgg agaggctctg gcctcaaggc tggtccatct aggtgcccac 121380acaacctagt gtctgcccaa aggaatagca acagcaataa gccatcagga tttaggaaag 121440gcacagccca atctaggatg aacaggaaag caatgagaag ccaggccggg agctttgagc 121500tgtgtcccca ggccaaacca gagaaagcac tcagtctctc agccccagac aggctaagag 121560aatccagagt atcacctggg gacatcagca ctaaggaaaa cccagacttg tagataatca 121620actctaacca gtacagcctt gtttctggat ccttatggcc ctggaatgag aagtggtaag 121680gcccatagct agtgctatag cagcctctac tggccacctt tgccttctat gctgtgccag 121740gagccctgca tcctagttgt ttgggatggt cctgtgaaca cctgttgtca ttaatgctga 121800ggactcactc tcagaaatac cccagtacac acatgacttc agtagccttc agtcacagcc 121860gagttccatg tcccagaatg ccttcaaggg catagagaaa taggtggtgc gttaagatct 121920ctgaccccgg cagcaaagca tctttctcat cagaccacag gagtgaataa ttcagagtcc 121980tctgtcctgg gccaggacta aggcttcttg acagcgccac cgtgaagaag atggaaagca 122040ggtgtgtggt gagcaggagg gaggggcctt ggcctaaaga gggtagaagg gatgagatgg 122100aagtatccag agggcaggaa gtgccaaggg tgtgacatgc tattgctgca tgtgtccagc 122160agatggcaac atagagcagt ctgcatcagc tcttggaagg gacctgcctg agccaggact 122220ggcttctgat gtcctgcagt gcagagcgcg ctctctctct ctctctctct ctctctcctg 122280ttcttggtcc atttcccccc agatgagtcc ttaggtaggc taaactacaa aatcgctaaa 122340gaatacattt cataagccct atgcctacct tcccaggaga ggaaaattgg aagagaagcc 122400aggcctatta gctccatgga ttcctggagc cctctgtctt ttcccagtgc ctgcgtggtg 122460caaagctcca agggaaccct ttttccctca cccactgggt cagggattct gagtccagag 122520atgaagagac ctgtgtgagc tgagggaatg tctccacggt ctgtcattca gagatgttca 122580tgccgattag cagcatggtg atgggcaatg tccacttacc atcaggatct gcagaatgtg 122640aggatctagg aacggcagcc atagctgcta tctcatagag ctctatcagg cggagagaac 122700ctgagctgct actgacagat ctccagcatc tcctccttct gagtcccctc caggtaaaac 122760agaggccaga ctggaagtct cagaaggcac accctgccta cacaagcata ttactatcac 122820aagcttgggt tgctgtccta gggccccagt cccagaccat taacttgctc tatggatcca 122880agactctcta ggcatgtgga tagagacaat ttggagtctt aagttcccta ggcagaagga 122940gatttttcag aaaaggaata atgaagatct tcaaatccag gaagatttga tgccttcaaa 123000ggtgttcagc ccacacctac acatcattca cgaactagtc actgagtctt caagtccaca 123060cttggttagt tggttcatct tgctttgtat tgaaggttgg cctcaagttc actatgttag 123120ccccagctgg ccccaaactc ccaataatcc tcctgactca gcctcccaag ttgcagagat 123180tacaagcata ggccaccaca cccaggtgca agctcactct tgcttgccca gttcttacct 123240acagagaaag cttatgctgg cctccgggtg aattcaacaa atattaaatg tcttgtatgg 123300gcccgacttt gggctggttg ctggagattg tggcctctat ctttaataaa ctctaagtcc 123360acaggcgaag gaaaacatga agttcgctgt actacagaga cctctagtag agttgggaat 123420ataacgcatc atggagtgag cccctgatgc cactgtgtca gcagcaaggg acagggagaa 123480tgtccaggaa ggaaatgaca tttgagcagg gtgctgaagg ctaagagccg gaggattgga 123540agtaaagacc atgctcactc agggtgtgtg tagtccaggg aggggcaggt aaagaagtcc 123600cacaggctgt gaaggctctc aagcaaaggc tgaggatgct ggaccttaca taagggcttc 123660tcccagaatc tgcttccagc aggtcattcc tgacctcggc cttctctctg gtgtctgcac 123720tcctgcctct cccctccccg ttctcacaaa agccacagac tccctgcctc tgggcctggc 123780aaagctactc cataaatatc tgattggaca gttccctgtg ctaatgtggc agttatgtga 123840cccagcacac acaggaccat cagttagcct agctaatgag atctcttcta aggagctgga 123900gagcaaagat ctgtatgcac cctggtcacc tcccacctgc agttcccacc cagctggtta 123960tgaacccaag gaccccacaa tccccaaaca ggataaacgt cgtcttcgga gtcccgtgaa 124020gctcaggaaa gactcaacca ggaagcagat tctgtgcagg gaacactgca aacgtctaaa 124080gtagaactgg gtgacagggc cactctctag ctgtgacaag ctgggcattc acctagtaac 124140ccaggtggct gcttgagtgg tgactttgcc acactttcac aaggaggctt taaactggga 124200ctcagaagct aagttctggt taggcatggc ggctgctgcc tgtaatctta gcacactggt 124260ggtggaggcg agagggtcag gggttcaaga ccgttcttgg ctacatagca agttcagagc 124320cacataaaga aaggcacctg tggccaagct tgacaatccg agttccatgc ctgggaccca 124380cgtggcagaa ggatagaggt gactcccaca agttgtcctc tgaccacaca cacacacaca 124440cacacacaca cacacacaca ccataaaagt ttgttttaaa agcactgatt cacttagggg 124500cagcaattat accccagcag ataaagcaca ttccttaaag ggctgactat cagagttcga 124560tccctggagt ccacataatg gtagaaggag aaaaccaact caacagagct gtcccctggc 124620ctccacacat aggctgtggc atgcacgttc cacatcatac acacatgcac aagaatggta 124680aaatttacat tttaaaacta ctgactaacc tctgagcctg tgggaggtta cccagaccat 124740gctccgatgg aggctccctg agcttgctga catccttcct ggctcacaaa agtgttagct 124800cagctggaaa tttctacttg aaaacgagtt agtctgtttt tataagccct gggatgggta 124860attcaaacaa atggtgaagc cacagggaga gacaattgaa aatggctagt gttgctccag 124920aatggaatat acatttgtcc caagaggggt gacagagtgg ggagtgttca actgtggggg 124980agtagcattt aacagataca gctctgggat cggtgcgggg actcatggct gcagtccctc 125040tcctactcca tatctaggat tagtgaatca acactgtcat tccatgcacc agggtgggac 125100actttgatca aataaagaac aacagactgg gaaggaaggc cgaatgaagt gcaacccagt 125160gagtcatttt ccagactcaa ggccctattt tgtgattgca ggttctgaaa aggagtcagg 125220taccggcaaa ccaacatcac tggcagaatg cagctcaacc ctctgtctca ggaaacttgg 125280tgggtgtgca gggctgggcg gactggaagt aaggacagga aagacatatg aggggtcaga 125340gcttggatat gctgtacacc ctcacagaca gacagacaga cagacagaca ggataaacag 125400acacatacac acaggacagt cacacacaca cacacacaca cacacaccta tggttctgtg 125460cataaatgta tagtgaacta tgactcactg tatagtcagt cacacgcgca caaagtggaa 125520actcacagtt agacctgaga caagtaacag agataaggct cagcaatctc aaggcctgat 125580tatttccaca gtcacccatt cccctagatg ctggtcataa caggctgttg ctggggacag 125640agctgcctcc tgttcctctc ctgaagccag tataacatat agaaaagttc catggcagcc 125700taagacaata acctcagagc taatttctgt atcactcagg agctggggaa cagaggacaa 125760agtgagcagg cacagtgggt cacagccctc ccctcagcta tctctgtatg ctctgtgact 125820gtgggtggct gtcctctggc catctcccaa cctgatatcc cacctcgtca aaccttcttt 125880aaccccaaaa ttcctccctg cctctaatgc tggtagagga caccggcttt tgttctgtcc 125940ccacagacct caacttacta ggtccctgct acagttgcag ttgcccacat actagcagct 126000aacagcctaa gacatctgtt tctgtctgag gattcactca ctctcatcct gtctgcccac 126060tctacttgac acataccaat caaaaatgcc cctttttggg ctggagagat ggttcagtgg 126120ttaagagcac tgactgctct tccagagctc ctgagttcaa ttcccagcaa ccacatggta 126180gctcacaacc atctgtaatg ggacctgatg ccctcttctg gtgtgtctct gaagatgcta 126240cagtgtattc atataaataa aaataaatat ttttttaatg cccatttctt taacagctaa 126300aggctatctt tcctttcttt actggtctct cttgagcctc aaacacctag ccttccactt 126360gggtctgata tctgaccaca aaaaatcagc tcacagaccc atccttcctt cctgtcacta 126420tggaaccaga gtggcctctt caaggttctg ggatgtatac ttgtaacttt gatgaaagca 126480aggaacaaaa gccccaatgt tctcctttct ctccccctta ggagacatgg acaacaccgt 126540gcctactcca atgtcactga aaggttgtct tgcttgactc tccaggagcc cagtgtcagc 126600catctctacc gtaagctacc cattcgccac agccacaaca ttcaaggcca aaagaaagaa 126660ccaaaccaat cctggaatgt ctaactgttt ttgcagcttg ggatgtagct cagtttttag 126720agcaagttcc tagcatgcag gacgtgctgg gttacatttg ctgaagaaac caggcctgta 126780ctgtaatccc atcacttggg aggtagaggc aggaggatca gaaactcaag gctgcctttg 126840gctatgtaca gagttcttgg ctaaccttag ctacataata aggctctctc aaaagaaaag 126900gaaaaaaagg agggagggag ggagggagga acggatgcaa cgaaggaagg aatgaacgaa 126960ctgtatgacc gaatgagggg acaaaaagac gggcaaccat cagggaaaag ggagtcaggt 127020atgagccgct ttatcgctac cgtagccact gaccaactac ggactggtaa gcggggcggg 127080ctaaccgtaa aaccatacct taatgccgac ctataagctg cccccctaat ggagtggtca 127140ccgccacgaa agatctaggg actggaagag gcttcagagg ggggagcccc gtaaaaaggc 127200cggggtgacc gcggagttcc ataaggtccc ccccccctgg caaacttaag ataaatcgac 127260ccccctagtc ctagggggcg gcaacccccc agggcttttc ctaatccccc cccgttcccg 127320ctgaaagaac cttggctggc ccccgttccc ccctgagcgc taataggaag aaaggccgcc 127380tctaccctga ggcacccagg gggctttttt aatgaacgta aaagctgagt tggcgcgtac 127440aaaggaatcc ccaggcggga ccaggtgctc acaagcctgg atgcccccta aggcccggcc 127500taaggacccc caagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 127560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatggc 127620cactgtgctc taaagccgtg tcgactagac aggcctcaaa gtcacagaga tccacctgct 127680tctgccttgc aagtactggg attaaaggtg tgcaccacta cacccaaaat tttgaagttt 127740atgcagccta tggcactgct agcctcaatt gagaaaaggc atgccatact gccctctagt 127800gaccagccta ggaaatgaca gccagctgca aacaaggcag acagtggtct ctggcattct 127860aaggcagaga aacagtatac atcttaatca aggtcaacag gcacccagaa tagaatcttt 127920attctcttgc tacacttcaa tctctgcttg aaaacatagc aatcccctta gaaattgtga 127980gaccttaaaa ctgcagtgag gacgtaaacc tgcctgtgga gaagaccctg tacagaagag 128040ctatgacgac aggctaggag tagaatgtgc tcacctctgc aggtatccaa ggggatggcc 128100tgacggcctg aagattcctc ctggagtccc ttggaggtgt tttaacacct tttaaaacaa 128160tttttaaaat tatacctcta tttccttgtt gtgtgtgggc aggtagggtg cacgccatgt 128220ggcctatatg gaggtccgaa gacaacttgc aggagtcagc tccttccctc caccatgtgg 128280aactggggga tcgaactctg gtcatttgac ttggcagcaa gcgccttttt cactgaacca 128340tcttcctggt cttagaagtc ttgtcttttt taggggcaga gggtaattct acgcttaaag 128400atatttgtag agtcagcaca atgtgttctt tcctctaagc ttaacatcct agtaacgaaa 128460ggacatcaga aaatttagac agagcactgg gaatttgtat aaactaaaaa ctagatttac 128520cattaggttt tccattaata atttgttttg gccgattttc gggttttttt tttttttaaa 128580gacagggttt cgtgtagccc taggctgacc gccaagtcca agaaaacctt gaactcctga 128640acctcctgcc tatacctccc aggtgatgag cgtatagcct catctggttt ctatggtgct 128700ggagattgaa catagggtac tctagcaact gagctacatc cctagcccct tggcttctca 128760gaggacaaga tgagcaagag tggtttagag aacaaaccag cagctctggc cccagttcac 128820aatctaactt gtagaatttt ttcatttcat aaatactgat catatacata atcatgttct 128880ttctctctcc tccccctcga ccctcctaaa taacacggta ccaggcttgc tggctgtggg 128940atttacaggt ataatcttct tacagaagta acagaagccc aaggacaaca cagctgctgt 129000gagaagcctg tgctaacttc ccatgggggc cagggtgggg aggggccctc atttattccc 129060taagggatag agcactgact gacttggatt ttgagatggt agcctaaatg agccttggaa 129120cccccatctt cctgcctgtc tcccaaattc tggaattaca aatatgtcac catggtctgg 129180cttctgtctt cactgttctt ttttaaatta cctttaattt tgtgtttgtg agcatgtaca 129240cgtgtgagtg tgtgaaggct ccttccacca tgtgaatccc aggaaacaaa ctcaggttgt 129300caggccattt cactgcccct gacttaaaaa aaaaattcca ccctaggaat tcatatcttt 129360aacaacttcc ttagcaataa atagtacctg cgggctggag agatggctca gcagctaaga 129420gcactgactg ctcttccaga ggtgctgaac tcttcttgag ttcaaatccc agcaaccaca 129480cggtggctca caaccatctg taatgggatc tgatgccctc ttctggtgtg tctgaaagat 129540gctatagtgt actcatatgc ataaaataaa taggtaaatc cttaaaaaaa atagtacctg 129600tattactttc ttgattcctg ctgtccaaac tacatagaga tattcatcat gtaactaatt 129660catacaggtt cctctgaagg gcatagccag gaaactactc tttttgtttt tttttttggt 129720tttttttttt ttttatttga gacaaggtct ctctcggtcc aggctggccc atgatcctgg 129780tctttctcca tctacctcac aaatactagg attagagtca tggatctttg taacaagtta 129840atgcctcttc catgaaaatt acctacctca gaggttgtta gcagctagta acaataactc 129900acaggaagat atctgcaaat catgcaatct ggctaaggga ccagttacca aaatacaaaa 129960tctactttgt ttctttagtt tggggtaatg aaactgagac ttgcacatgg cagaagccta 130020ggcaaacact ctaccactga gccatactcc caaccattcc agaaacaatt ctttacatga 130080aagtgtgtac aagccgagta tggtggcaca cacctttaat cccagcactc agtaggcaga 130140cacaggtgta cttctgttag cttgaggcca gttttctaca tagtaagttt ctgaacaacc 130200agagctatac agtgagaccc tatctacaaa aataagaaaa aaatgtgcat atacatatat 130260ttatacacat atgatgtaca tgtgtgtata catatgctgg gtccttatag atacatgaaa 130320aatgggcgaa gtcccttaat agacatgaag atctataaag atgctcaaca tcaccatcca 130380ttatggaaac cagcattgca gacttaccac ctcctatcca ccaggatagc tgtagtaaga 130440acagaagtac agacaagatt gcagggaagc caaaactctc ataaactctt gggaagagac 130500ctggcgctta ctcaacaagt cagagcatag atttccatag gactcaatgg ccccactccc 130560gagtccgtgt atggatacac actgatagct gcagagtaga aaatgtttct ctttgcattt 130620agctaaccgg gagtgaggca atgggctcac tggctgtcta ggtttgattg tctccatttt 130680tctactttca cacttgtctt ttccttcctg ctttgtagag ttctgtcagg tagtcaatcc 130740ttcgtgtctg caatccctcg ctgttacaca ttgccggtgt cttctcgatg tctgtagtca 130800tccttaactc cgacatgacc tgctctgctg cacagagcgc ctggcttatg cagcagcccg 130860agcaatgagg gtgaggtttg ccgtgagctg tcctcacact gtacagccgt cattgctccc 130920accgggcttt actacctgtt tgagctcaga agcccagagc ccatctgagc cacactctag 130980cttcgctgta

cagttcacct gggtccttaa ttgcttcagg actgctgatg tggtatgaga 131040caggggcctt cttcattttt gtccatataa cctaattttc tgagcctcag cggccacctt 131100tcccactaac atgtaacact accttatcac acatacagtc caatattcct gaacttggtt 131160taacaccaaa ttctgatttt cacagaacac ttttacccac acaggtaaat taatgaaaac 131220tgcaatcact caatactccc gagacaccca gaaatttcat acagctggaa ttcttcccaa 131280gcagaatacc cagttaggaa atgattataa aaaaataaat atgtgtttat ttttaagaaa 131340aaggaaaaca ctggccaaga acaaaccctg ggataaagaa gtcaccgtgt cctgcccaag 131400ccagccacag acagtgaggg cacagccagg ttaggtgcct gtccaaaggt caggctgaca 131460gcaacttgct ggtctgcgtc tcttagcagg actcctttag agctggatgt gagctggtgc 131520taacagcacc acagcatcac atggcctcag gggtcacatg gcctgggggg ggggtggtgt 131580cacatatcct ccacactcca cttgtaggca agctcctcca cagccttctt gctggcgagc 131640ctggcaaagc gcttctgttc aaagccattg gatctgcaat caaaagagaa attctagtca 131700gccaaaactc tcaacgttta ctttaaaagg aaggaagcca ggtggtggtg gcacatatct 131760ttgactccag caatcaagag gcagaggcag gcagaggcca gtctggacta tagagagttc 131820cagaactgcc agagctacac agagaaaccc tgtctcaaac aaacaaacaa aaaggaaagc 131880tatttatagg aaaatattta aaccatatgc taaaaagtca aagcaacaaa acacacaaat 131940taaccctctt ctcagtcccg gttcagactt acggacaact atcagacgct tagacaacta 132000tttcaaacct ttattattct ctacaaagat tcaacacagc tcggcctggc ctgcatgggc 132060tgcggagtta agttcatggt taagctggac aacttcacga agttttgcct caaaataagt 132120agtaaactga agggacctaa ctcagtggca atggcagagc ccctaccagg ctatgagctc 132180aacacacggt gcaaaaccaa agcacaactc acttgtggcc gatacccaca ggctcaattt 132240aaccgttggt tacataagta ctgagttctc actggacatc cgggtgctac ggaagaatca 132300gggcaggaaa atgaactgca caatacctgt gctgatgaac tcagacaagc gacacagaca 132360tttaactaaa aagcaaaagc cagggctgaa aacgtggctc gggtgttaaa agcatttgtt 132420gctcttgcaa acccaaaatg tgaacccctg gtaccattgg ataggctcac aatcaccttt 132480aactctagcg ccaggggacc caagaccctc ttctggatta tagacactac tgacacacag 132540acacacaact gcgactgaag agatggctca gcagttaaga acactggggt tcctctggaa 132600agcccgtatg tcagctcact accatctgta actctagtcc cagggaatcc aaagctactg 132660ctgccctgag ggtggtgcac agacacaaag ctaaaacacc cataaaaaca aacaaaatgg 132720aagagttaaa ttaaaaaaaa aaaaaaaaaa accaaataaa aggaaaaact gttagaaaca 132780tgtgtgggat atcaaagaaa cagaagtggg ggtggggaaa tatcagatga aggactaagc 132840aagctaaaac acccataaag acaaacaaaa tggaagaatt aaattaaaaa aataaataaa 132900taaataaaag gaaaaactgt tagaaacatg tgtgggatat caaagaaaca gaagtggggg 132960tggggaaata tcagatgaag gactaagcaa gggcctttgt gggagtagcc ccaagctatg 133020cctgaaagca gcagcaaacc cgcaggcaca gaaaaaagag ccctggtctt cgaaaggggt 133080gacgggtgtg tgtgtggggt cggggggtca ctggcatggg cagagcctag agtgtgacaa 133140atggtgtagc cacaagtcaa cactggaaag acaggtagag gccaaatccc tgtgagctta 133200gacaaactgt aaaaacaaac aaatacaaat ttcatcccag aaagatggac ttaaaactgc 133260atttcagaca tctctagaga tgtgcagtaa gaaagctagc acagcggcct gacaacagat 133320gaaggcagtg atgccaggta gggatggcaa caggggccgt cgagaggaaa gagcagtagg 133380actgggtgac tgactagtca cagcctgggt cagtaacctg agagagcaga tgagtgaaga 133440tgccacctgc aggcgggtat gctttgcatg tgtgactgcg cctgaggcac ctcaagtgca 133500caagggcaga ggatcggact atgacatatc tggacccaca cacagacagt ggctgaaggc 133560ctggctcaga aaagaggcaa ccggaaggag caggagtgag agcagggagg ggttccagaa 133620ggagaactga agaccactga aggttaacag agaaaggaca ctcacagggg ccaagacagg 133680accgagaaca ggaacaggtc attatggaag ccaattctgg atgaggttca agtacatggt 133740gagtggacca caaacttcaa attccttccc cttaccccaa cccttttctc tctccaaaaa 133800gccacccctt ccttttttac tatttattat tttatattta ttttcctttg agacaaaaca 133860ctgcgcgcgc gcgcgcatgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgc gcgtatttag 133920ggctccaaag acagccaaag aaatgaagag cgcagcaggc acagtgcaac aggcacggtg 133980cagcaggcac ggctatgaca agatccacct gcggctatgg agcaagtgcg agttcaggga 134040aattctcctg ccaaagattc tgcagggtga ccagcaagca cagcagccag ccagcctgca 134100actgcagtcc ctccttgacc tcaggaagaa ctagagtcag attccagaag cagcctgaag 134160gacagaagac aacgccttag gctggcgcaa gtgcaaagtg gattctgaca ctcaaatagc 134220aattataaaa cctgagcaaa ataagtagtg gtttaaggca tgtgtgagcg ctaaagcaga 134280aagaaacatc ccaataggag ggggttagcc agagggtacg ttctcggtcc acacagctca 134340agtaggccac aggcagaacc acaggctgac tggcttgagg tattagtggc agtgggaaga 134400gtggctacaa aattaaggag aagtcctggg agagagacac tggttatgta cagcaaaggt 134460cacaccaagc tttggtgact cactatgtat atgcagagga cctgtagcag ccctgtcaga 134520gtgcagcagc cagaggcaag acctgagccg agatgttagc tgctgcccac tgcaagggag 134580acgggttcag tgtagagtaa aactccagac aaaaatcagc agagaaacgc agcaggagcc 134640aaagctccac tatgtattcc tcataaataa aagacctaac aaataagcca actggaaacg 134700gaaaataaca tgtactcaaa aaaagttagc cagttacagt gtcagtcaca cataatctca 134760acagtcagga agctatggta ggaaggtctg agtttgagat cagtttgggc tacatagcca 134820gctctaaatc agactggcct acagagtgag accctgtccg tcccccaaag agaggggagg 134880caggggacag gttattaaca aaaagcaagt ctgtggtgag cagaatgttg taatcagcag 134940acaaaacttc aaaacaccat ttataaatat tttccaaaat gagaaaacag tctctaaatg 135000ggtgagcaga tgggaaatct cagtgcagac atgcaagcca tcagatggga attctggacc 135060caacgcagct gagacataac agcaactgag agaaaacaac taaggtatca ggacagttct 135120gtaaactctc aagccacctt agcctaacag agttttataa agcaccatca tcccaagtaa 135180ttgctgaatg tatattaagg gcatcagtca ctagggagaa tcaaaagaat gggaaaatat 135240tctacatgta tttagagaac tttctcaatc taccactcaa gtaattttct gatggtgtat 135300aataaaaacg tcagcatttg gaaggtgtgt aaaactcagc caacctgtaa gttacaaatg 135360accaacatat gattcaagtg tgcactggta aaagatgtaa agaataataa agaccatgac 135420tggaaagatg gctcaggagc tgctcttcca gaggacctgg gttcaattcc tagcacctat 135480ctgtaattcc cgtcttgagg aatctgacaa ccccttctgg ctgccaagat tacttcctga 135540acagacttcc atgtaggcaa aacacccaga cataaaaaat aaagaggaag aaaatgatca 135600tgaattgtgc aggcatggcg gcacatgtct ttaatcccag aacttggtag gtagatctga 135660gtccaaggcc agcctgtcta tatagggagt tccaggtgag ccaggactac tagagagacc 135720tgtctcaaag gaaaaaaacc cacaaagatt ataaattgta atgcaaaagg atcaatagtt 135780ctctccatgg ctacagacca cacaacagcc ttgaaggaga cagctcttct gtgtgccaca 135840ccttcctctt ctgctggtga catggcaggc tgggttctcc tcctgcattt cagctcaaca 135900cttcacaata gacaaattca gaacccagct gtctactatt agtctagtca ttaatgttat 135960ttgcaattaa aattattttt gtttttttaa taattagttt tcgttaaaaa tagtggtaat 136020tatattagta tataatggtt tattctatat agtgaataaa tgctctttta atttttaata 136080tagtaactat aaatgtataa taaacataaa caaaagcttc ttggggtctc caaaagcttc 136140tttaaaaatt ctatttgttt acagaggcac acacacacac gcacacgcac acgcacacgc 136200acacgcacgc acgcacacac gcacgcacac acacgcacgc acacacacgc acacacacac 136260gcacgcacgc acgcacgcac acacgcacac acacacacac acacgcacac acacgcacac 136320acacacacac acacacacac acgcacgcac gcacacattc aaatgagtgt gtggaggtca 136380gagaacaatt tgcaagaggc agttctctcc tgctatatgg gtatcaagac ttgaactcac 136440atcattagtc ttagcagcaa gcgcctttgc ccacagagca atctcagtaa cacagagtat 136500caaggggtct tgagattgaa aactcggaga accctcacac tacacgctca tgttccatgt 136560ggacaaaggg agatgggatc tgcgacaact ggcagaagcc atcccatcag tcaggaagcc 136620cgagtacact ctgagacgct ggccagtaac cagatgaggg ggcaagactg gaacattacc 136680cggggctccc tcccagaatt acagagaatt aaatgttcaa acacaacatg ttaaagacat 136740aacacagaga tggctcagca ggtaagagca ctgactgctc taccaaaagt cctgagttca 136800aatcccagaa accacatggt ggctcacaac cacccttaat gagatctgat gccctcttct 136860ggcgcttact tatttataat aataaataca tcttttttaa aaaaaaccac aaaatcgggg 136920cagttgtggc gcatgccttt aatcccaagt actggcagag gcagaggcag gcagatttct 136980gagtttgagg ccagcctggt ctacagaatg agttccagga cagccagggc tatacagaga 137040aaccctgtct caaaaaaaca aacaaacaac ccccctcccc caaaacatac aaaaacacct 137100acaacacaga aaaggctcaa gaccacagcc cttgtccacc ctctaaccct tagaatactg 137160agcatgagca atgtggcaaa caaagtgggg cttgtccgtc actcatcctg agcttttcca 137220agtttatggg aaggagttgg gagaaaccca agccttgaaa taccgagctc aggagctgaa 137280gcacgagttg cttgctactc cctgtgactg cagagtgaga gcaagggtgg agggacctta 137340gactctggac aaagcctact gtggccccca ggctctatcc ccatgaccta ggaggtagtg 137400cacgagttgg agtcagccta gctaggaagt gagactctgt ctcaaaaagt aggaaaaaat 137460aaatataaaa aagaaaaaga aaaaaaaaaa ggaaacgaaa accagaaagg gtcaaggtca 137520ggcagaagag aaaacaaaaa tataggaaat agactcacct gtccactcca tcccagcgat 137580acccaggcca aatgttgaat ctattgggag gaggagcagg accactgtag cggggcttca 137640ctaaagacag gaatgagaaa aaagaaatta gctactgaca caatttaatt cagagctaga 137700tggctatggg agaaaattct tcatggaagt tcctattatt ttgattcaaa cttccaaaca 137760caagggcatt acagtagtgt ggggcagccc tctgacaact ctcagtcacc tccagctcca 137820gcaaccccaa gtctcacctt tcttattctt gttctcctta gctttattct tcttaataaa 137880gttggccatc gggtcccctt ctctttcttg ttctctcagc atccgatcca gatcttcatc 137940atctatgtag cgggccagag gcttctgcat ctccttcatt gcatcttcta cattctgttg 138000ctgctgccgg ctctgggcaa gcctgggcaa aaaagaagca tcctgcttga gaattctctc 138060ctatactgag agcccacagc gctgccctgg aggactccct ctttgtagca gccccagaat 138120ccggacgggt agcttgaacc aaccatctca ggagactgtg cacctgaggc tcgccccgta 138180tcagctccca gaaatgcaag gcagaggctg gctcaggtct tgcagtctag ctttgtgctc 138240aggggcgccc ttaccctttc ccccactggg catatagctc atctcgctct gagtctttct 138300ctgcttttct cctctgttcc aagcgctcta gcttcaaatt cctcttccga ccagacttgt 138360ctcgaaatac agtttcagtg aattcaaact gtgctatgaa ggaaaaaaca cattttaaag 138420atacacacca ggcatcacag tcagaaatca cattctagca ctagaaacca actgcttggg 138480atcttttgct gaaagaactc tgcagacaga ccatcctacc agcactcttt cacatactgg 138540gaaggaaagg agtgcttgta ctactgaggt aaggtacata aaagcaagga agtttccgat 138600agcaaataca agccacatta acaacagaaa agagagctat aggaccttca gtggcccctg 138660caagctcaca attgtacctc cgaggtctgt ggtgtcctgg tcttgtttct tgagttcctg 138720gtgctcccgc tgaacatcag tgaccaaccc agtttttgct ccggaataca tgtgtgcagt 138780ctgtgcaaga gaaaaggaat acatgtgtac agcctatgca aggggaatac acgtgtgcag 138840tctgtgcaag agaagaggca acagcaacac catttttcca ggaggcagat agctgctcca 138900gtgcctgact gttagctata accaaaccaa aacctctagt tttatattct cagtattgac 138960cacaaacagg gctcacaaca tagaatggga aatatctaat ctcatagaac actttatggt 139020atcaaatttt aaagtaaaca gaaacacaaa catggggcac atagaaggga gtaagaattt 139080gcatgacttc tctttttttt tttttttttt aaaaaaaaaa gatagagtcc cctacagcac 139140aggctggctt tgaactcact tatgcacatt ttaaaaagca aaaagctgac atttagagga 139200atataatttc tcgtccaatc ccacagatac taagtagcag aagtagaatt tacagctatt 139260attggtttca cagggttctg cacttctaac tgcactttat aatctgtaag cctcaattta 139320ctgttaggaa aacaggtggt gccagcctgt atatctatca acaggtggga agaggcagat 139380gggtcagaag ttcaaggcca gccttggagt acactcaaaa caacaaagac aagacactga 139440gatgagactg gtggcattac aaggctctga gaggcagctg ggcactcctg atccaaaacc 139500agaaatgcac aatagtccaa aatctacaac attctgagca ccatttgggt gcagaagcac 139560agccttcaaa ctccaacact ctggttccca agcttcactg aggcccagct aagacgagtt 139620ccacccatgt caccgtcaga gaaagttcca gaaatgcttc taactccaac ttcctgtacc 139680actttctcat tctctgctgg aggagtcacc ctgataaaga aagacacaac tcctggaaac 139740ctaccttctt gccaggacgg ggactccttc gaggtggaga gaggtcagaa tcagaggatt 139800tggtcctctg tcttctccgg ggtggagaga ggtcggagtc agagctctgc cgccttggtc 139860tgttccgtgg tggtgacaga tctgaatcag aatcctggtg ccccgaattt ttttttttcc 139920gtggaggaga aagatctgaa tcagaggctt tttggtagac acctaagtag aaaagaacaa 139980aagcgtctat gaaatctcct gtgtctaaga gaatctaggg attaaagaat gaattaagga 140040tggggatggt tcagcagtaa aatgcctgcc tagtacgtca gacctgggtt tggtcctcag 140100cacaggagag aaggggagga ccattaatct ccttaggaga ttgcttaaaa gaacttacca 140160tgctgggcac ggtgtctttt tagccatcct atgaaataac atgactaacc aaaacacagg 140220cacaagtgac acagagaaga acaaaggcgg gggcttggtt tcctggctac cagtgtctgg 140280tgactttgaa aagcgtatga gatctcagac acagtctctc actgccaccc tcttcagaac 140340tggcatcaga aatgagttgg cccgtgcggt gtggcagagt caggaagacc tggactatat 140400gctgatctcc ccagactcaa gaaaacaaag taatttcatt ttaagattaa aactatagct 140460aaaacaaatg atgaagtaaa acaaaaccct ttctgcaggg gcctgttcta gatgacagtg 140520tcaccagtca cctcctgaaa agccactata tagcaggtac taggtagtag caacagccac 140580acattctgac ctagtaaata actccatagt tttctaggaa cctatcagtt aaatccccag 140640gagatctaag ataagaaata atacaggggg ctggtgagat ggctcagtgg gtaagagcac 140700ccgactgctc ttccgaaggt ccggagttca aatcccagca accacatggt ggctcacaac 140760catctgtaac aagtgactcc ctcttctgga atgtctgaag acagctacag tgtacttaca 140820tataataaat aaatctttta aaaaaaaaaa aaaagaaata atacagggct agagagacag 140880ctcagcagtt aagagcactg actgctcttc cagagctcct gagttcaatt cccagcaacc 140940acatggtggc tcacaatcat ctgtaatggg atctgatgcc ctcttctggt gtgtctgaag 141000agggcaatag tgcactaata tatttaaata aataaataac tcttaaaaaa aaaagaaaaa 141060gaaaaaagca gcaatcccag ttgtgctatc agtgtatgta catgatgatt gtcaaatatc 141120ctcagggcaa actcagggaa gactggtaag tggagcgagc aggcaagaat ccacaggcaa 141180caagtatgct ctgtacactc aggcccaact acgcccaaca tgcgtgccca aaccacctaa 141240ggacaccggc tacaacacta gcaatgacgt tgtctaaatg atacaatttt gtgacactgc 141300tgtgtctata cattcctgta cgtcagtgta tttattatta ctgtatgtgt gcatgtgtgt 141360acatgatcgc agacatacac atgcggaggt cagaggacaa ctttcagggg tcggttttct 141420ccttttactg cagtggtgaa gacaatgctt taccctctga gcctctgagc ccaccccatc 141480tttgcctttt tatggggagg gtgagtgagc aaaaggacat gaaactttcc ccccaccctt 141540ttatttttca actcccctaa gtctagtctc tcttttttca aaactttcca aaagaaactc 141600attcttacac tccccaggag gaaggtcctc ttatgcatag ccttatgctc acctttagaa 141660tcaagctgtt tcttagcttc aaaatgagcc tttgcttgcc gtttccgtgg aggagagagg 141720tcagagtcgt gctcgtactt gctgttggtg gagagtgatg ggtgagaggg tcctagccca 141780ctctgggatg cagccttgct aggggctctt tctgcagctt tactactttt ggctttgtgc 141840agttccaaat caggggactc atgatgggcc cttctgagat gctgtgtacc tgaagagtca 141900agagtccttc tatgctgggc tgcagtaggg gagtggttat gggtctgtct cggagaagag 141960gtacctaagg aaccatgatg gcctctccta ggagatacgg ctgaagaatt acgatgagac 142020ttcctggggg gagaagcatc taagtcatgg cggactctcc tgggaggaga tggatccggg 142080gtgtcatggc ggactctcct gggaggagat agatccgggg tgtcatgacg aactctcctt 142140gggggagata gatccggggt gtcatgacga actctcctgg gaggagatgg gtctggggtg 142200tcatgacggg ccttcctggg aggagatggg tctggggtgt catgacgggc ctttctggga 142260ggagatgtgt ctggggtgtc atgacgggcc ttcctgggag gagatgggtc tggggtgtca 142320tgacgggcct ttctgggagg agatgtgtct ggggtgtcat gacggaccct cctaggagga 142380gatgaatctt ggtcatcgtg atgaaaatgt ccatcttcac cgtggcctaa acacaagagc 142440aaacagtaag agtcccacaa tgtgctctgg cctaatggca cagagggtaa caaaccccac 142500ttaggagaca attcttttgt cacctatgta taagaaaaga gcaagcacca ggaatattct 142560atagttaaat agatctcaag aagtaagatt gaatcacagt gacctagact atagactgag 142620accgtctcaa aggtttaaaa aaaagaaagg aaggaaagga agacacaggg gatggaggag 142680aagatgggag agccttgttt atggaaagtc aggagaacag agaaatagtc acaaagtgaa 142740agacaaaact ctggctctga ccacaggagc tgtttcattc tagaaccgtg gaaaggctgg 142800tctccaggct ctgacaagtc agcctctaat gttccatgcc cttcttctaa agtcctgaca 142860tctttgtccc atcatgtttt tcatcatcac tgacataaaa caaaccaggc attcccctaa 142920ggcagggaag cagaaggaac ccatcctgga aatgagatgt gcagttgtgt tgctgctgac 142980actcacctcc cagaagcttc cacttggcac tggagcggaa ggcctccatc tgctttacct 143040cttctggacg ctcatccaca aactcagcca cctgcaggag taggggcact acagtgagga 143100aacccacagg acccagccaa ggatcacttg tctcaagaac ccagagccat agcttacagg 143160cagagaactg cctgcttctc cttgtgatcg catgactgac ttctactgcc catcatgaga 143220ctatcatacc aaacagtgct agccgaagat caaaaattca aaaccatttt ctgctgaaaa 143280tgtatcatgt ccacaccact gcacagtcaa aacattaagt tggagcaggg tgtggtggcg 143340cacgccttta atcccagcac ttgggaggca gaggcaggtg gatttctgaa ttcaaggcca 143400gcctggtcta cagagtgagt ttcaggatag ccagggctac acagagaaac cctatctcga 143460aaaaccaaac caaaccaaac caaaccaaaa aaaaaaaaaa aacattaagt tgggactgtc 143520tgtctgtata agcaccatga aggaaacagt gccacacagc tctcttgagg gtcacctctc 143580cccttgctca gtgtcaagtc tgagaagcct ggatcatttc aaagtcctga caaaagtctc 143640cacgagatac ataccacagg caaatctcca tcctcttcct cctccttttc cggcttagca 143700gtagagatag ccgcccagcc cacatcatca tcaacaatcc gcattctaga tgagaacaat 143760aaaaactcaa aacggaagtc atgctgtaac acgggcctac ctacctgttg ttctttacag 143820atggttgtga gccaccatgt agttgctggg gattgaactc aggacctctg gaagagtagt 143880ctatactctg agccatctct ccagcccctt acctgttgtt ctttgacatt tagagtgtgt 143940gtgtgtgtgt gtgacagtga tggtgtatat atgtgatatg gcatgcatat ggaggccaga 144000ggacaacctg caggagtcag ttctctccct ccgctatgtg ggttcagggg atggaattca 144060ggttgtcagg cttgtcaagt gcagagccat gtcactgccc cctactggct tttaaactta 144120aaggtatggc tcaatgtttg tgtgctgtca attcactcca tcactgtcca cctttggtaa 144180tatcagtttt tacaaaggca cccccttcct tctgccagac ctactcttct cactgttaat 144240cacgaatctt attttctttg ggccctgtgt attagtcagg gttctctaga gtcacagaac 144300ttatggatag tctctagata gtaaaggaat ttattgatga tttacagtcg gcagcccaat 144360tcccaacaat ggttcagtcg cagctatgaa tggaagtcca aggatctagc agctactcag 144420tctcacgcag caagcaggcg aaggagcaag agctagagct agactccctt cttccaatgt 144480ccttatattg tctccagcag aaggtgtagc ccagattaaa ggtgtgttcc accacacctt 144540taatcccaga tgaaaggtgt agcccagatt aaaggtgttt tccttaaact gggagattca 144600atcttctgga atccatagcc actatggctc aagatctcca aaccaagatc cagataagga 144660tctccaagcc tcaagataag ggtcactggt gagccttcca attccggatt gtagttcatt 144720ccaaatattg tcaagttgac aaccaggaat agccactaca ccctggtttt taataacttc 144780cttggaccta tctttcttgc tcactgtctc ctactcccag agtagaaata cctctacaca 144840ctcttagaat gctttcattt gtatttatca gcccatattc tcattaaatt ctaagttcca 144900acagccaagg gctaaggcat tctcttattt attcccttct tcttgttgtt gttccctata 144960gctagcttcc agctctctat cctccagccc agcctcccct gtgctgggat tatagacttt 145020taccaccata tcctgctgac taatacattt tttgttcatc aaggcttctt caacacaact 145080gtcacaggag gtattcagcc ctccacaccc atggacttca caactgcaaa ctcaaccagg 145140ggaaaaggaa tggccaatgt attaaacaga tagactttct ttagttacaa tggcctatat 145200aacgtaacta ctggcatact acctaagctg tagtgggctt ttaagtaatt aatctaagga 145260tgatttaaag aggatgtgaa tagttgacat gaaaatagtg tatcattttg agcttcagga 145320accagtgccc caattactgt aaggtggtgg caagccaata aataaatgtg tgccacacag 145380atgaactgtg ctgcaaggag gaggttattt gaaatgccag gaacacatga cacagcttta 145440atcacaacaa ccatttcaga caagtagtaa ggggctggga cttcaggctc agagagatga 145500acatctctct cttttttttt tttttttttt tggttttttg agacagggtt tctctgtgta 145560gccctggctg tcctggaact cactttgtag accaggctgg cctcaaactc agaaatccgc 145620ctgcctctgc ctcccgagtg ctgggattaa aggcgtgtgc caccacgccc ggctgagatg 145680aacatctctt gccctggacc actcaggcta taagtagcac agctaggatt ctaattctaa 145740tcagtattgc tccaaagctt aaactttccc cctgcatgcc attgcccctt cttccaggga 145800catcagccac actgaattca acaccacctt aactgaatat aggctcatct taactggtaa 145860caccacaaag attccatcta taaatgaggt catgctcaag ctctaatgaa gagaagtgtg 145920gaaatgaggg actaattcga cccagttcaa tggaccatac ggtaataata ctgttagacc 145980atgatactgc cccacagaga gggtgagcca cactttgtat cctatctagt actctcttta 146040agctgataag

catttagtag acctgggggt ggagtgtgag aatggaatta gcccccagag 146100tttcaacttt ctatccaaaa tagcaccttc agtaagctgc taaacttagg gctgctgctc 146160taaagcctac aaatacagac atttcctttt gttctgtagg ttgatgttgg atatcctaac 146220ccaacctttc cagattccag tggccaagtt actaatggac atttcatctg aactctgtct 146280cagcacatct ctttgcaatc tggaagtata gtgtttaata taaatgtctg ctccattaca 146340atgttctgaa agctatatgg tcaactattt tagaaagagg ccaggcacag gatgtgaact 146400gttcccattt tagttgttac tgttagtagt agatgtgagt aataggatat gggtctttga 146460ttccagatca gttcctggca ctttggaacc ctcagaaaga gatttttgga tctagaaaga 146520aagaaagaaa gaaagaaaga aagaaagaag agggaagaga aaaagaagaa ggaaagaaag 146580agaagggaag ggagaaagag agaaataaag tgagaaagaa aaaggtttga atcagctttg 146640ctattcttgg acacgacttt caatctgtac atggaacgac gtaagctaca aacgcgggga 146700gaaagtgagt taagttctag ctgcctttaa cacaaggaag gcgctgctca gaggatgagg 146760tctgagctgc cagtggcagc tacaatcccg ccccggggca gtggagcggg gcctgagctc 146820gggccggggt cggcagcacc gcgcgggcca ggacgaggaa gagctgcggc cgagggcgcc 146880accgggcagc gcgacacctc accagtccac tcaccccttg ccgccagcgc ctccaggctt 146940cggccgtttt ttgcgccgct tgcgaccggc ctcgggacct ccttccaagc cagcatctgt 147000cccagacaag taacgcttca gatactcagc tttggtgagt ggcggagctg ccgccatggc 147060agcggcgagg gcggggcaac agctcggcgt gggggacaaa attcttcaac gcgaaacggt 147120aggggtggag ccagaagcgg aagctttagg acggggactt tttgaactgc tggacttaaa 147180gacttgagcc ttaagaggct ccaggggaga gtgcgtgcat ctcgggctgg ctgtgctttt 147240cctgagacga cgaatcggtg caacagcctg cagcccacgc tgtctcagct gagaagccac 147300aggccgatgt aacatgatcc taccctggca gcatcagcgc cagagtaagg aagaacacct 147360gtatgaatgg tgggacttac catagctgta ccactagggg cacattcttg agcagcgttt 147420taataatgga cgctgtacca aacgtttcaa tttcaatcac ttttattgct ataaatacct 147480tttggtggga agtctgctgg aagaggcttc cgggtaccat ttaggggaag tcaaacttgt 147540gactataaaa gtttgatagg tttttatcaa acctgtactg accttctcac ctttgatatg 147600tctttctaaa aaatgatata aactcttccc agtgaaatgg attcgtggtc agtgtggcaa 147660agaaaccagg aagattctgg agtttcttct ataaagggtt aagtgtttta tgacttctca 147720cttaaccctc acaatttttt tttctttcat ccagcaccga ggattaaacc caaggccttt 147780tgcacatggt atgcaggagt tctatagaac tacatcccca ctcttttgaa tgttgaagat 147840ctaaattgcc taagctgccc ctgaatttgt tagccttcct cctcagcctc tcaagtggct 147900ggacaccatg acctaccctt cagttttatt agcctccttt atagataata agaactttcg 147960ggtaagtaca ggaacccaca catcttcata ctgcctggct ctaatttttg ctagcagagg 148020ccaaacaagg atggtgctgg ttatccagtt tgaattcgtt ttgtcttgaa tcagggtttt 148080tctgtgtagc ccaggctgtc ctggaacttc ctctgtagac caaatcggct tcaaactcat 148140agaaccacct gcctctgctg ggattaaagg tgtgtgccac cctgcttttc cagactgctt 148200taagctgcat gagaacagct agactccaca caggtgtacc tggctcagag cagacaggat 148260ggcctattgg agtgaatcaa atgctgcctc tacagcagcc acctcaagaa agatttgctt 148320aggaaactaa actggttttt ccttcctcat ttcttttcat gaagacggac tctcactgca 148380tggcccaagc tgtcctcagt ctcctcggag ctgagtgtga ctcagtgtga cccagcttag 148440tctagcacat ggtaatcagt aaggacatga acgcagaggc gggtgtcgtt taaaggccat 148500cactttaatg tggcgtcttc acctgtgcct gctcggcccc tccgacatcc ttccctgctg 148560agtcagtact tcacatgccc agagccagca gaaacttgta tttcacttcc ccagacttca 148620aaggtccagc actggaggtc agagagctga caggcaaacg gtgatggcag accgcctggt 148680gtttctggga ttcttctcac gccgacttta aaacctccaa aagaaggtca aggagcacag 148740tagggctgca gctgcctcgt ccgtcagctg ctaatcttgg gtgcttacat ttctaactca 148800agtggtaacc tcttcaccat ggacgagggc ttgagcactc ctcgtcagtt ccccacagta 148860acagataacc ctgcagtgtg agtggcactg tagctggcca gtgagctggc cactgctacc 148920gctgtggggc caggcccgcc tcatagccct ctgtcttcat gtcattgagc ccgagctcct 148980cattttggtc aaaggtgcgt ttgtaccgct cgaccttcat ctctggatcg tcttcaggtg 149040catacacatt ctgcagggcc aagagcagac agacgacggt cagcgcctgt gcagcccgcc 149100atcccctccc acggctgact ctcaacctcc gaagaaatgc acaggatttg cagggtctct 149160agagcccacg gctacattct gcaaaagtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtat 149220gctagtatca aacccaagcc cctgtacatg catacaagtg tttaaccccc agttctaagt 149280acttttatat atgggccttc ctataaaaaa tgtagccaaa gttggtgatt gcttataatc 149340tcagcactct ggagactgag gcagaaagat tacaaattta aagccagcca ggctacataa 149400caaagacttg tctcaaaaaa aaaaaaaaag gttttgaggg agggggacca caatgatctt 149460aattatgtga aaaactaggc cagttttgcc aggcccatca gtgctcatta atactaaagt 149520aaggaggagg ccccaggttc aacacaattt tgctgcagtt tgatatggat caattgccag 149580atgtttgctt gcccgttcag ccaacacctc tcggctttcc tgtcctgacc tctggccagg 149640ttcctattgc tccttacttc acagtcagat gcagaatgga agctccaatg ctgagggtga 149700gaataagaac aagacaagca gacccaggaa gaggaggtcc tactttctta tatattcact 149760ctgtacctag ggccctgagc acgctagcca ggtaaacagt tactcttggc tttttcttca 149820cattattgta tcttaagaca gtcttgaaaa tcgcacaggc tggtcttaaa ctttccattt 149880tcatggcctt ctgaggacct aagattgcag ccctgcacta ggtctttgtt acacacactt 149940gggagtccca ctatcaccac cttccccttt cttccttacc ttccaagtcc tgcccaaggc 150000cccaattgaa gacttaacaa ggctttcctt gttaagggct atcatgcaaa ttctataaag 150060ttccgaaaat atgtctccat agaaccacca ggaaggccta acttacctgc agataactgt 150120ttcctgctgg atcattcatg ataaagtggg ccttcatctt gccctcgatg atctacagga 150180gagaggagga cttagcaggt aactcttaca aagatcctgg aaggaagagg ctatggctgc 150240gagacaagag ccaatccagt ttacaaagtg cagttcccaa aagtttaaag aaagggaaat 150300taggattgca gaaatggcct gggtcctctt acctggccca acttctggct aaactcctgc 150360agtttctctg actggtcagg attagagctg tcgcccagtg tgaatgggtt cttggttacc 150420tgtagcacaa cagctggatt actacaacac agactgtatg ggtcattacg ctgaaccaca 150480aaccaggggt ggggagcagt ggactcccag tgggagaagc ctgctctcca tatcccacaa 150540cgccttgctc acttagggga ggggaggaag gtggcccgag aacaggacag gagaagatgt 150600ctatgagact gctttgccaa gaaggaaact ggcagtaggg actggaatcc agtagcgttc 150660ttacagtatc taatcccaat atggaaaaga aatgcaatgt acaattcaca aagaaaagcc 150720aggcagttac ttggcctgca gtgccagcac atggaaggct gaggccgaga ggtggcaggt 150780gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtggagtgac ctcaaggcta gcctcaatta 150840tatcgcaaag ccatctctca aataaataaa ataaacacat ttataagatc taatataatt 150900ttgcactact gtttaggaaa taccgacaag aaaagtctcc acatattcac tacagatgtc 150960aaaaagaccc ccttctattt tgtaatgagg taaatgactt aagatcacaa aataactaca 151020ctggcccact gctagccttg agaatttcca ttaaaaattc ctataactgc tggacagtgg 151080tggcgcacac ctttaatctc agcacttggg aggcagaggc aggtggattt ctgagttcaa 151140ggccagcttg gtctacagag tgagttccag gacagtcagg gctacacaga gaaaccctgt 151200ctcgaaaaac caaaccaaac caaacaaaca aacaaaatcc tataactaat ttctcttcct 151260tctcttcata attaatactc aaatctcttg aatgagcttt tttttttttt tttttttttg 151320tttttcaaga cagggtttct ctgtgtagcc ctggctgtcc tggaacttac tttgtagacc 151380aggctggcct tgaactcaga aatccacctg cctctgcctc cctagtgctg ggattatact 151440cgtgcgccac cacgcccggc ttaaaccagc tttaaggcaa agaaatcccc agttataagt 151500gacaaaacag aatttggttt tggtaggagg catttgggat agggtctcag gctgtcctca 151560aatcctgtct tcttgcccca gcttctcaag tgccatcata cccggacaaa caatgttacc 151620ccttcccact gccaaactgc taacagaagc gatgcagcac actctgcact caccagttct 151680cggatgtctt tcagcagtcc ttctagagtg gtgaacttgc ccccaagtac agccattccc 151740agttcaaact caagctctgg gatttccaca ctacaggtct cagactataa gacaacaggt 151800caaatttaca attaaatcac aggatggcag catagcttca tccaagagcc cttgtctagt 151860attaacagag gtcccaggtt caatccatag cactagacag atggacagac atacagagag 151920acaaactgat ttaaaactag gaacaaagaa agccaaccaa attaataaac cagtgacagt 151980ttgaagacaa agttagaatg ttagtttact cttaatacct ggctaaaagc cagggcctca 152040ctcccccatc tgaaccaaca cgaagttaac ataacaacct gaaactgagt cacacctaca 152100agctattgta ccttgtcatt agggtcagag aagagcccat atagccaata gaaactaaaa 152160agcagccacg tcattgctca ggaagactgg ggccaccttc tgagtctgta gtcccagtca 152220tggaaaaagc ttcctcaggg tcactcaagc ccaggggagt tacagggttc cttgtgttct 152280taagcactaa gatgatcact tcctctagac tgaagcggct atacgacaac agctattttc 152340caagtaggct accttgagga ggtctctggt catgtctgag ggatctgtga tgtggagggt 152400gatcctggta cccaaaggtt ctacagctcc tccggacttc acctgtggaa acaacggtca 152460ggagcaggtc cagagaagag cccaggcctc acctgctgcc acaaagactt gggctttatg 152520taggctccca tcaccagctt cccatgacgg aagctcctaa tccagcaagg gcatcagtga 152580cagaaataga aaccatcaag agtccacaga acactgctcc gccctggtgg catgccaact 152640ttaggcaact gccaagaaac cctgaatata ccttaaaaag aaaaaaagaa aagaaaagaa 152700aaaaccctga gactgtcaga ggatgaaaga ggcttgtaag catttcagtc catccctgca 152760tttgctgtca taatctagga atttagtcag ggctgggaca tggctcacta acagcgctct 152820tgccttgtct gcatgaggcc ccaggtttgc tgttcactgt gtccccataa cttaagttta 152880atcagcttat gctcgataca cttaggaatg aaggcttaca acctccacag cagccaccaa 152940atccattccc gacagctccc ctgagttctt cactgagtca cactctgtcc aacctccagc 153000cttctgagaa ccacaacccc ttccacaagg tcagttttcc gtccatcttg caatcttgta 153060tttccaactt ctctctaggc taattctcag ctccttcagt ggctcctccc cagcactaag 153120cccccaaaag acccaaatac agctgatgcc catcctcaag tagattcagg aagctggcag 153180actgctacaa aggcgttgcc cctacgccaa tcttctccca cccacctcat tagtccgatg 153240cccacaattc tcacagttgg tggccatgat gataacctct ttaaagtggg ggatttctgg 153300aagatggagt taaagaagtc tatctgcttc aaggaaaaca cccccacccc cacccccaag 153360gaaaagcaaa gccaagtccc acacaaacag atgcaggacc caagagtaca ataaacgggc 153420ctgtgctcac aaacacggag acctggccac actcagaagc caggtatgca gtatgtagct 153480tcattaaagg ttcatcctca ccactgagga taaagcactg gctttccctg aagggaaggc 153540tcccgtctgt aatataacgt tcatgcccga gtcagctgaa gagcaaccca attgcctgct 153600gaaagatacg gacaagcttc atgttggtct gagccggagc gttgcactct gggcagttag 153660tgttgaactg gagcacctag gacataatat aggggcacag actgagcatc cccacccctt 153720cgagctgaag acatcccgca ggtggtcacc catgtgagct gtgatggtga cacactggac 153780attcgtcagt ctctacccac aactccacta tggtcttcaa agggagccag caccactcac 153840ttcgtttcta aggtcttcct cttccgcctt ctcctctggt gcttctgcct gaaagaacag 153900aggcccaaac cacaagttgg cagatagacc tcagcctaca gtagagccct gccctactta 153960aagagggaga gaatgccttt gttccctgta aggtaccaaa ggacacctca aaggacagat 154020ggatagagaa gccaaagcaa atccccagac aagagaaaga cggggctcaa ggctgggttc 154080ctgtgactga cacctgcttt gtgagcgtga taaaaggccg agacaggatg cattggatga 154140tggtggcagc aatagcaaag tgacttcagt gacttcagga cccagaacac agggcagccc 154200cagagcatcc ctctgactta atcagtccac ttacttggag ccccagcatc tcagcttgct 154260gtggggttcg gtcatagtat gtgatcacca aggcattatc tttctgggga gcatgtgggt 154320tttctacaaa gctgtttccc gagggatcat caatgacctg acaaataaga gaggagagag 154380taagataaac catatataac ttccatccag cgccagaaca cactagaaaa atcctgctcc 154440acttttttat ctttaggttg ggcttcagca tagcctagcc tggcctatac cgtgctatga 154500agtcaaggat gagtccaaac tcttgatctt cttacctctt ccacttgcca agctccatgg 154560ttaccagcat cagtcagtgc caagggatgc tcgcctcctc cttacactga aagtggctgc 154620agtagcccag tacttaccag tgtgaaaggg gaagccattt gctttaggtc cttcagtttg 154680ccaatgaact catcaattct ctctgcgatg gcaccttcca ctgcctgtca ggaacaggat 154740gaaaactgca gacttggcag tacaccccac accccaaccc tccaacagcc atatccctcc 154800agtcagatcc ttaacatcca cttagactgg gagcacaggg acctgaattt tatagtgaga 154860cacaagaagc tcatctgaag tttataaacg atgctggggg ttaggaggcg ggcttatttt 154920cttatttatt attccactgc actgtaactt cgtctcagtg gggaactgtt gtaacagtgg 154980ggaactgttg cttctggctc acaaaggtat ctgccatcac atttacatct tacatcacac 155040gggcacataa cacataatta aaaagaaatt cctttaaaaa tccaaaattc tgttatgcct 155100ctctagccag ttagagacag acagcagcaa tgtgcaatga cattatttct ataataacaa 155160gtacagatat aaaagaatcc tcaggaagct gtagagaagc cgaagactct tcaagataat 155220gtgacattat ctgaagtgca ggagggcaca ccgagacgtg ggctggggac agcacaacat 155280ggagaactag ctcctttatg tgctcaatca aaaagttccc caacatttgg tctgatacag 155340tttgactcat ccctactcac ccgtcgtgtg ggctgatcct gttccaggcc agagatcgca 155400cggctgatga gtccttcaac agtggtcaga gcttgtggta aatgaaacat gagacaagtg 155460agtgctgacg tgctatcact tgtctgtgtg accctcaaca ctttccctga actctgcagg 155520cctccttctc ctcatctata aagctctaaa ctaagcacac cgtggtctgt ctcactactg 155580gactcttaag agatttaatt agacgggcag tggtggtgca ggcctttgat cccagcactt 155640gggaggcaga ggcaggcaga tttccgagtt tgaggccagc ctagtctaca gagtgagttc 155700caggacagcc agggctacat agggaaaccc taaaaaccaa caaaacaaaa caaaacaaaa 155760caaaagagag agagagatta actataactt tttactcaca ttaaaacatg aaaatccact 155820ataattaaat gaccaaacac aagccactag gcgtgacctg gaccaggctt tgaaagaaaa 155880taggaaacat ctcagcttgt gaacagctca cacacctcac acacctgatc ttaaaactca 155940cctcccttct ggctgaaggc tggaatctca aaatccagct cggggatcct tgtggtggca 156000gagtctgtct ttaccacttc tctgttcatg tcctggacag aaagcaaacc gttctagcta 156060aagaggtcag caataaagct tgggaaataa ccagaccatc tctccatcag atgccaaccc 156120agatctttct tcttcccgcc ttccattcag aagagtccaa actacttcag gatagtacac 156180atggtgtcag cctgactacc cagaaggtaa gaacaccacc cacaaagccc agctcttcca 156240cataaatccc atcactccca caaaattcgc aaagcacgtt caccactcaa agcagcagaa 156300ccgaggccag caccaccgac accactgtca aaggacgaca ctaagggctt gggacaggtc 156360tggtgaaggg ctgatggtcc gattgggggc tctgaccccc agtgcgctct ctgaaaccgt 156420gggggtacct tctctggtct gcgctcacct cttggcttct cacggtcaag gtgtagcgca 156480ctccctggtc ctggatcctg cctgcagact ggatctccgt gttgttccag ccacagtgtt 156540cgcaggaaaa ggagctcacg atgatttctc taaagaaggg gatcttggtg agcagaagtc 156600gcgtcgtgcc ctagggaagc agaagcggcg ttggggagaa tgacttcagc ttgtttacac 156660cctgtacgcc agagctgcct ctgggtgcac ggctgggtgg ttccccgggc tcagactcac 156720gttccggtaa cagttcatgc acagtgactc gatctcggtg ggctgttgct cctcatcctc 156780ggcgctgagg ggccggaaca aaggcccggt ggctggtgac gcagccgcag cgggcgaggg 156840cccgacggcg gcccccgggt gtcccggctg cacagccccg ctggcagaca tctccccacg 156900cgttgctccc gcggcggctt ccggcttccg cggcccctgc gacctcactt cctccagccc 156960ctgttcccgc cccttcttaa agaggccgca gaccctgcgt ttcctaggtc agtctctgac 157020aaagacttcc ggttcctaga ctgcacttcc gtaactagag aagacccaca ctgggctgga 157080gagatggctc atcgggtaag agcactgact gctcttccaa agatcctgag ttcaaatccc 157140aacaatcaca tgttggctca caaccattca taatggggtc tgacgccctc ttctggtgtg 157200tctgaagaca gctactcgtg agtagctcgc tagttgtggc ttctctatac gggtagcctg 157260tttgtcccca ggactccagg tctatgtaaa atgccccggt tgctttggaa tcgcctaggc 157320tgtgccccca cgggctggtg agatggctca gtgggtaaga gcacccgact ggtcttccaa 157380aggtccaaag ttccgagttc aaatcccaac aaccacatgg tggctcacaa ccatcggtaa 157440ggagatctga ctccctcttc tggagtgtct gaagacagct acagtatagt tacatataat 157500aaataaaata aataattaaa aaaaaaaaac tgtggccaga ccttgcctgg gtcccctctc 157560ctgttatcta gcttctcccc tccctgactc tctttccccc agctcctcac ttctgtcctt 157620ttaaacaccg cccctaactc taaatacact gctcttcttt ttactcattt ttctcattcc 157680ccccaccgca ttcgctgtct taatctctcc ttgtttccta acaaaacacc ctactttatc 157740gggttttttc aaatttcctt tctagcgctt cctctctaag tcttggacca caacccagtc 157800acatggctga cactgactct atctacagga ttgagggtaa caagggtgtg gttgcgtgga 157860gtgcagcttc acagaggatc tgtagtgggg aggtatcgag catcctgtgc agagaaggct 157920gagctgggaa gaaagggtgc tgagctgggc tttccttccc cgcccctgtc ttgtttcact 157980ggtccctggc gatctttgat atctgagtga gtgtgtatac gaaagagagt taagagttct 158040taggtctccg agcatgttgt taaccctagt acttaggaga cagaggcaag ttgatctcta 158100ttaatgtgag gctaacctga tctacataat gtattccagg ccagcaaata ctctgtagtg 158160agatctgtct caaagagaga gtttttatca atcccaagaa gagagtcaag caggcatgag 158220gaatctggaa catgggacca agggtaaggg gatccttcag ggatttcagg gagagcatgt 158280ttccccatta cacactccat cacatgttct ctgcatcatg ctctcctcct gctcccttcc 158340aacaaaagct ctcatgacag ccctggggag acaggagttc ctctccgtgg accaactgta 158400acagccagca ccaggctgcg agaattaatc cacgctgtcc tgtgatccgt agactgtgga 158460acaagacagc ctcctcccca cccaacccca tcaaatgtga cagccattgt ctagccagga 158520gtcctacggc agaactcgcc cagagtggac caagaatgga aaagagacag aaagagcaag 158580tgaggggtac aagtggatgg tgggtgtgtt ctgagacaga ggggaaacaa ttgctctcag 158640acatggtcca atgagagtca cgccagacca acaacgttcc atcacgagca tctatttcat 158700tccaaccaaa agtcagttcc accctgagag tcaggaggta gggactgtat gatgaggaag 158760gcacacagtt tgttctgtac aggatttttt tagaagccct ccttcatcgt gtggatggct 158820cagttcctta tctggattca gagttctcta aggaaatagg ggctctgccc ggtcccatca 158880ctggacccac cagcaggacc actggcaggg acgtacacac tccatcacat gttctctgca 158940tcatgctctc ctcctgctcc cttccaacaa aagctctcat gacaaccctg gggagacagg 159000agttcctctc catggaccaa ctgtaacagc cagcaccagg ctgcgagaat taacaggagc 159060ccaactccag gttgtagtgg catgggtttc cagcacggtc aagcactggc ttgcggtcag 159120tcaccccaca caccatctcc cactcctgtc acctcactct tgctacttag gattgccaaa 159180tgagtaccag aagctgggtt tgcaaagcct actgagtgta atgcccctga gtaggggagt 159240tgaggaggct gggtaggttg ggacagcagc acctttggtg gtgtgcagga ccctccaccc 159300tctgcctaat agaccatgct agcggctcag ggtccagaca atgagctgga gggttaaccg 159360gagtgaccct cagggtcact caaatgacta tgaccctggt cttgaaggcc ataggcaata 159420tcttcccaca ggtcgtccag tcggctctgc agtctgctca gggccttatt actgtctgag 159480tgtcccagct caggagcgaa ggcgctgtgg ctaggcgggg gtggggctag ctggtgctga 159540atttcctctg tctcctggtc aatggcctga gtaaatgcag caatctgcag gtaggtgtca 159600tgccgaaaag cctgcagtct ctggcggacc tcctcggaga gagcctgagg gtccagggaa 159660tctccatctt ccgccccatc tgtgctgacg cggatgaagg cactgagctc atcgcgcagc 159720tggtccaggt tgcgttggat gctggtgtgc aggtccttcg ccttacgtgt gagtttgtgg 159780gacagagtct gcacgcagcg actgagtcgc gcggggctgg cagctgcgtg aggagcgaca 159840ctgcggtgca gctcctgcac gtggtgccca attccagtca ccaagcgttc tgcgtaaggg 159900tggaagagct ctttgactcg gtcggtatgg tgcagcactc gactctgcat gtcctgcagc 159960aggttcagcg cctcgtccac gccccccagg agctgagcct tggtgtcttc tcccaccacg 160020cgcagctgct cctgcagctc ctgcacactc aggcccacct gctccatcag ctccgccgtg 160080tagggtttca actgctgcct caggccctcc aagttccagc ctacctgctg gtgcttcgca 160140gccatgtagg gctccaggcg cgagctcact tcccccagct cctgctgcag ctgtttccga 160200atgccttctg ggtcctgggc cagcagagga ggctccttcc caggccctct caagggtccc 160260agcttttcta ggtaattgtt catattgtag aggtcttgct cgaagctgcc tttcaggttc 160320ctggagaagg agaaagacag agaatcaggc cattagactg ctagcattga aatctccctt 160380tccccaaagc tgtgggacac taatattttg cagaagccag gacaggagga gtggccccag 160440agcctggtct ttagtcctga ccccagccaa cttcattgtt acattcagaa cacaaaatgg 160500cattcggtct tacaatacca tccaagaagg tgtcacacac acacgccaag attcacacca 160560tcttgtcatt ctgtggcatg ctaatctcag gtgccatggg caggagaaac ttggacaaga 160620gggtgcccaa gatgactgag gtgtgtttgg ttctataggc agggctggtt ccttcagctt 160680ccatgttaac cacagagcca agggatcatc ccacagtagg gctgtctcgt gccctcagca 160740cagccttgcc caggctactt actcctgtgc cagcttctgt ggctggccca tcacgccttt 160800gctccaactg ttttggctga agtagtccca gaggctcttc cgtgcctgag tgcttgcgaa 160860cactgcagag gaagatgaga cagttaaggg ccaggcccct cagcccatcc tccatcccaa 160920acccacactg cagtgggcca gaagacccac ctgcgaggag ggcgagggcc caagtgatga 160980ctgcagccat gctttccacg atggcccctg agaaggaagg tagatgaagg cttgatcagg 161040aggagaggca aagccaggga ctagatctag gaagcctggt ctagggggtg cctgccccaa 161100atcccgataa

cccttcccca gtgtgtaggt aacatcatgt caaaagctcc agagaactga 161160actaggcact tgcggggact gggggtgggg gagtgaggca ggagggaaga gatgacttcc 161220tcagacaaca gggttggatt gctccttacc tcggtcacaa agtagggaac ctggaactca 161280ggctgcccta gagagatgac ccacccacct caatgcaatc tagctccctc gtttgcctgt 161340cttcctgcct ccgagcccct tttagggatg aggactggta cacacaattt ctgctagcct 161400gtatctgcag ctctctccta cctttgctgc caccccaccc ccaccatccc tgtgccatgt 161460aagctcacct gctcggttct gggcacagag agcagacact cttactttat actctaggcg 161520ccagggagtc tgacaacaat tcctctgagc aaacaccacg tggagggtca aagaaggatg 161580accccagctg gttactagca cactcacctt ttccccttct cctggcactg ttggggttag 161640taagctcccc agcctcaggg gggctgcccc agtagacaag tggctccctc gagcctttac 161700taagtgtggg cagaacccac cccccaaatc ctgtgaccag caaccgcccc acttactgaa 161760tgactggcca gccaatgaac actgcttgtc tgtccactgc gggccctgcc tttacttaac 161820tccccagcgc cttctgcact agccagctgg ccagagggac agtgtccagg actggctaga 161880gcctgggctg gccttacttg tcccaccatg gccactgtga cagcctgaga agagccagta 161940cttacagggt tacagaccta tggcgcctag agtataagtg tctccaggat tgagctggag 162000aagtcaggtc ctgttgaaga tggatggggc aagctgcatc tgggtcagag actttgggta 162060tcagaagtta gtcccctcaa gagtcttccc tcacagggaa ggctgggctc cacccccttg 162120agcttccgag gagggaagcc gggaagccca gatgtttctc ttatgacaag cttcttccat 162180aggcttgcat ggacatacct gagtgcttca tcctttctct tcaacctcca gtcggtcccc 162240ctgcctccac ccacacgctg ttcccgtcac tttcctcaga acttccctct gcccttcacc 162300gtgaccagtt cagcctcctc ccttgtctcc acacaaaact tctgccatcc tcgatccact 162360tacctctcag gcacaggggg atgtctgcct ataatcacgg cactggggag gctgaggcag 162420gagaattgta agtttgaggc cagtctgggc tgctgtggga ggtcctatct caaacaacaa 162480aactaagaag agaaaaagaa gctgccaagc ttcccactct gtcccagcac atgggtcgtt 162540aaagaagggt tgtaagctgt acttacctaa aatatacata gtccagagat tgatctcaaa 162600cacggcataa aacaggatta caggcgtgtg cttggaatcc tgagacaggg aaactgcagg 162660gacctgaagg ggacaggaac tctagaatca gaatggagcc tctcacctag gcaagaatac 162720ctacaccatt accatgtgct atacattagg tttcaaaact ggggcagaag gctgaggtgc 162780ctatttaaca tatcaaagtg ggtctggccc ccacattcgc cctgttacag gaccctcctg 162840gctggcatac tccaccccta ccctaggctt ctccaaccca gggagctgtt ctaccctata 162900taatccaacc attttggtta cccacttttt ttgtgtcttt gtgcctcccg gttgcacccc 162960tctctccccc ccccccttat ccctccccct ttcctctctt cccatgtggc ccagctcagt 163020ctggcctgtc tagtctcttc cagatgtccc cacctctggc tctgctctcc catatatcta 163080ctataaactg tctcctccac cacagagcta ggatcagtca tgttcctttc ctttcgcctt 163140ttttattttt attttttcat tcactataaa ggctgctgac atagctacga tttctttctt 163200tatagatgct ttatgttaaa atagagacgg agatagaatc tcaagcaggg actccttgct 163260tttacccaga cactgcttga aaatctcaaa cactgtttac aacaaaggta ccctcctttc 163320ctatatcccc atggactaga cttttgaatc aattattata ctgataagat agtaacgtca 163380aacctcatcc aagtcctatg tatgactgct gtaaaaacag tgagccacgc ctctcttgag 163440gagcccacac ctgaacacat cttacatttt tctgaatatt tatttctcct aatattcatg 163500tgttaaccta tattataatg ttttagtaaa cttctggtcc agttctaact tactgtatca 163560gctagtctta tttttatttt ctttttagaa gattgttctt aaatatctgt atatatgtgt 163620ctgtgggttg gggggtgcct caggaggcca gaagagggtt tcagatcccc tggaactgga 163680gttgcaggca gttatgagcc aaccactatg agagctgaga cccgaaccca gatcctctgc 163740aaaagcagcc cgggctctat cctgctgagc catctctctg gccagccccc gactgttaca 163800acacttcatc cctgcacaga agacttggtg cttgaagcat gtggaatggc ttctcttttt 163860tctggctgaa cctagacacc tggtgaatgt aaggcatagg catgctttct tgtttgccag 163920caccgtgggg cttcagtgtc gatgtgttca gggtggttgg aaatatgtgt gtatttgttt 163980tatgatctgt gaaaataaaa tcagttttga tagcttaaaa ataaatgatc aagggctagt 164040gagatggctc agcagttaag agcactgacc aaagccaggt gtggtggcgc atgcctttaa 164100tcccagcacg tgggaggcag aggcaggcga atttctgagt tcaaggccag cctggtgtac 164160agagtgagtt ccaggacagc cagggctaca cagagagagt tccaggacag ccagggctac 164220acagagaaac catgtctcaa aaaaacaaca acaacaaaaa aacaaaaaca aaaacaaaaa 164280caaaacaaaa aaagagcact ggcttctctt ccgaaggtcc tgagttcaaa tcccaccaac 164340cacacggtgg ctcacaacca ctcataataa gatctgacgc catctcgcca tctcctggtg 164400tgtctgaaga cagctacagt gtacttacat ataacaataa gtaatctttg gaccggagcg 164460agcggggcca gagcgagcaa aggtcctgag ttcaatttcc cagcaaccac atgatggctc 164520acaaccatct atatagctac agtgtactaa tatacataaa acaaataaat ctttttttta 164580aatcaaatgt tggcaatttc atgtctaaca agatctattt atatgtaatt tagtatatct 164640aatgcataca gcgttcttag cacatggacc tgaacatacc acaaaggcag ggttgctaag 164700aatgcaccat attgatgatg ggactccttt gaacagtggg gaactaaaat attaaaccaa 164760agtaatgtag aaaaagggaa ggagacatca aagtgaaagt gttctccagt cttgctttat 164820ccatggagaa gataggaata ttgatcgtct cttgcttttt acatcaggca ccaccattaa 164880agttttaaag agaatccatt aaatgagtag aaagaaaggg taacttgcaa actggtaaat 164940ggggaaaaga gactaaagag cgtcgggttg gccgctagtg aaggtgctga ttgatcgcac 165000atgctcactt caagctgttt cctgaaagcc caataaaatg tcattcaaat gctcccctta 165060gagaagcacg aatccacgag gacagaaaag gagacaaaga cagccacatt ctggaaacgg 165120taaaacttct cggcagtcaa acagtagtta ctgaaaacac atgaaatgat ggctttttga 165180aatggcaatc cagaacgtta agaacttgct gggtttagct gttcagagac agcagcacta 165240gattcttctg gacatatggg gagggggagg cagtggcacc aaaagagcag cacctgggtc 165300aaaagacatg ggagaaacat gtctccttta gctctactta gtaaagtcag acacttgttt 165360tctagatgtc tcacctcgtg cctggggtgg tggtgcatgc ctttaatcct agcacttggg 165420aggcagaggc aggcagatct ctgactttga ggccagcctg gtctacagag tgagttccag 165480gacagccagg gctacacaga gaaaccctgg ctcaaaaaaa gggggggggg ggaattctag 165540actacagtca aatgctagtc ctaacctttt cctacttgtc tctgtctggt tgtttttgag 165600acaaggtctc aatgtgtagc cccaactagc ctgaaactca tacccctcta gcctcaggct 165660ccacatatca agttctgttt taagtgtttc aggtaagaat attatcaggg agaggtgggg 165720ccttcccaac acaccacact gggggaagca tatcatggtg ggtccttcca attgcctagt 165780tctgggggag cagagaaaaa gaactgagac tgcaaacacc cggaggcttc tgcgcaaacc 165840tttgtttaaa acttttttta ttgagatgga aaggtaggga ggaagacagg gagggaagga 165900gggagtgtgt gtatgttaga gtttctgtgt gtgtgcacgc tatcatgcac atagtgaaaa 165960cgtacagaag tctctccttc catcatgagg gatccaagtc aagtccgcag gctggacaac 166020aaatgcctct acctgctgag ccacctcacc agcttgcaaa tatcacttca tatcccaaat 166080tcatcagtta ccaagaatct gaggatgcta aaggtggtga ctctgtcacc tactaaagac 166140agaaaatgga gctagagaga tgactaagca gctaagagta ctgtctactc ctccagagga 166200ctcaggtgca attctcagca cccacatggc agctcacacc tgtctgtgac tccattccct 166260ggtcatctgc gggcaccaga cacatgaggt gtacatacat gtgtgcagga aatacactca 166320taggcataga aaaaaaatgt aatctttttt taaatggctg aggggagagt tgagacaggg 166380tctctctaca tagccctgac tgtcttggag ctcactaagc aggccaggct ggccttgaac 166440tcacaaagat ccaactgttt ctgccttcag agtgctggga ttaaaggcat gtgccaccac 166500acctggttta taattgaaaa aaaaataaag acaaaataaa atatcttttg aaccaaaaca 166560atttacttat gatcacctta ccccatggta aaatctccac ttgttcatag tgggagaagc 166620aaagtctgag ctgggaccaa gccatgaaca catgggcttc tgcggagcat aggtcattca 166680ggcccacgtt atagccacat aatatattat gccacggtga gggattttct gtgtctgttg 166740tttcattcat gtttcgagat gttagtttta ggtagatgct tagatgtggg attggtggat 166800taagcatgag tgttgggttt gtttgatatt tctcactaaa ggaattgttc tcttttgtga 166860gtctataaag cctgaataaa agtgtcttta gtttgtttgt ttgtttgttt gtttggcttg 166920gtttggtttt gctttacctg cctggagtgg tagcccgtgc ctgcaatctc agcactagag 166980agacacacac cacatttata cacataacac acacacacac acacacacac atacacatat 167040cacacacaca cacacacaca cacacacaca cacacaccac taaatgaata taataacaaa 167100agcatacctg acaatctgac acatgagaaa gtacatgaaa attcacgtac ttcctgacaa 167160tctgacacat gagaaaatct attttcatgt acttctgctg ttcccgttcc tcccactatt 167220agtaaagcgg gcatatattc tagtctgaat ggttatttga cctttctgtg actgttttgc 167280tcatctcttt cccttcattt tctactgaaa atcttatctt ttatattcac agcatatata 167340tatatgatga tctgtttctt attagtgatt gtatcagtca gggttttcta gaggaccgaa 167400actgatacaa tgaaatatat atatgcacat atgcaccata tatgatatat atggtttact 167460acaggctatc attcagctgg tctgacaatg gctgtctacc aattgaaagg ccaagaatcc 167520ggtagttgtt cagtccacaa ggctgcatgt ctcagctggt cttcagtata tgctggaatc 167580acaaagaaac aagctgtaat gccagtgaag gaatggattt gccagcaagg gcaagcaggc 167640aaagggcaaa aacttccttc ttccatgtcc ttaatatatg ttgtcaccag aagatgtggc 167700tcaggttaaa gatgggtctt cccatttcaa atgatttaat taagaaatgt ctttcacaaa 167760tgtacccagt tgggttgacg agatggctca gcgggtaaga gcactgactg ctcttccgaa 167820ggtcctgagt tcaaatccca gcaaccgcat ggtggctcac aaccacctgt aatgagatct 167880gacgccctct tctggtatgt ctgaagacag ctacagtgta tttatgtata ataataagta 167940actctttggg ccaaccagag caagcagggt tgacctacag gactacagga ttgaacagag 168000gtcctaaaaa ttcaattccc aacaaccaca tgaaggctca caaccatctg tacagcgata 168060gtgtagtcat atacataaca taaaaataaa taaatctttt ttaaaaatat acccagctgc 168120ttcagtttta gttaattcca gatgtagtca agttgacaac ccaaaacaac caccacagtg 168180atataagttt attggagctt gccagcaagt gcttccccga ccgagacaac tcgccagtca 168240ggttttctct tactttgctt actatgtttt atttgtttgt ttgttatgta gagcattgct 168300tagtatttat gccattaaat ttaccttgca tcattttgaa cttgggagtc agaattataa 168360caagactcac tgcttcctgg cattaaagga aactgcctgc ttttttcttc atgtcccagt 168420gtgaaaggtt tctttaatgg gtcaatcaat agtccagatg cttggcatag ccaggtgtgg 168480tgagacatgc ctttaatccc agaacgcagg aggccgaggc atgtaaatgt ctgtgagttt 168540gaggccagcc tggtctctat gtagtgagat tcccatctca aaaaaaaaaa aaaagcaaac 168600ttgagcaaga atgttgacat atactttaat cccagcacat gggaggcaga agtaggtgga 168660cctctgatca gatagagctg ccaagggact tctcccgcag ccatccaggc acctggagtc 168720cgtcgcagtg tggtccaagt agacctggct ctggctcctg ctaggtctac ttctggccga 168780ccttggagtc agccacgtga caagagttgt gcaatacaca ggatgggaga gttgtgggct 168840cacccagggt tccacacaga tttcaagatc tgggggtgaa ggtgggagaa agcatatggc 168900agaatctgaa tctcgtagcc ccctgggagg gcaatggcgg aaacagtgac agtggaagcc 168960atactgctgt gaccccagga agtcagagat gccagcagtg gaatgtctgc caaggaaagc 169020cacatcctgg gccacagtgg tggggctgcc caaaccctac atcatagcat cacatgccca 169080cagggtacag gatctgccat ttcccttgct ggggttggat ctggccccaa tttccatttt 169140tatgtctcta ttccatttgg atcagggata tttatctcac atcatatgga agtctgtaac 169200ttgtggggtt tttttgtttg tttgtttgtt tgttttttgt tttttgagac agggtttctc 169260tgcatagccc tggctgtcct gaaactcact ttgtagacca ggcttggcct cgaactcaga 169320aatctgcctg cctctgcctc ctgagtgctg ggataaaagg tgtgtgctac cacgcccagc 169380ttatttttat ttctattctt atagatgctt gttactaggt gtttgctgag agtgatgggg 169440aacactctgg acttggactt ttgtataatg atagaactgg tgagaatttg aggactatag 169500gaaacatcct atattgggca gtggtggtac acacctttaa ttccagcact caggaggcag 169560ggacaagtga atctctgagt ttgaggccag cctggtctac agagcgagtt ccaggacagc 169620aaaggctaca cagagaaacc atgtcttcaa caaacaaaca aacaacaaca ataacaacaa 169680caaaagagag agaagaagaa agagagggag ggaagaagaa aaggagagag aaagagggag 169740gggttctaaa tgcattttgt gtgatgagat ggatgtgggc ctttgaggga ggtcaggggt 169800aggatatgaa atgcttttcc ccaaattatg ctttgtctcc agtcaatgta cagaggtttc 169860ggaggtggag cttctgggaa gtgaccatgt cccagggttc taactgaatc aatggatcaa 169920ttcttagctg gttgtaattt ttgggatgtg ggacttagtt gtgtgttgtg cctgtgaagt 169980gtccaccttg tctctaattc ctctttctgt ctttctactt cttggctcct ctgaggtgaa 170040ccgttttgtt cttctttcca tattggtcca cttacccaca atcccagaga cacagagccc 170100gttgaccagg cactgaaatc ctggaaacag gaacccaaat caatcctccc tcctctagac 170160catttcttgg acatttcgtc aaagcgatga aaaatcaaat tgggttgggg agatgactca 170220gctgggtaaa catgaagctt cccaaaactc atgtaaacct gaacccatgt ctgtagtccc 170280aggatgggag acagagactg gagaatgcct gcaagttagt tcatgggcca gctagtcctc 170340aagagacagc tagagaccct atctcaaaca agatgtaagg caaggaccaa cagttgagac 170400tgctctgtcc cttctgcctg tttgtgtcac ctgagcatcc ttactcacaa actagagcat 170460gcacatatgt gcacacatgc acatgagaat taaacaacaa gaaaagaaac gttcattcat 170520gggctggagg tgtagctcag gtgtagagag cttgactagc agacacaagc tctggcattt 170580catcctggca ccatggaaac tggagatgct ggtgtacatc tgaagtcctc aaatttggga 170640ggtggaaaca ggcggatcag gagtttaagg tcttcctcag ctatgcagtg agttccagtc 170700cagcctgggc tctatggccc tattgattaa catgggaacg caaatatggt ccatggacag 170760catgctggtg gatagcaaag gaacgccata atcaatagaa aattcagagt gatttggcaa 170820aagtcgggcg tgaatcacag ccagatcaca tagtttgagg ccacagagct gcactgaaaa 170880gttagaatgt tgcaactcct caggtctttg ccctcatttg taaaatgaag gtgataccag 170940tacccaactc tagggctaca cactgaaata acgcttttta aacatgattt atttaaagtc 171000atgtggtgat gaaccacatc tttagtccca gcactcggga gacagaggca ggtggatttc 171060tgtacattta aggccaacct gccctacaga gctacttcca ggacagccaa gtgtgggaac 171120agggaccggg aaatagggtg ggggagtgga agcggagccc acaaagattc cctgggattt 171180ggactctgga gaccagcatc gaggtccagc tctaacttta ctgtttagta catatgctga 171240tatgcagttt ttcagccaat tagggtacgt ttatgtatcc agggacaaat ctgggtgcaa 171300catgtgttgt ttagctgaat ctgtcccatc tgatcatgag cctgtagggc aggtgcctgt 171360catccaggaa gatttctgtg aaggccaggg gaaggagcca ccactctgcc ctctggtcct 171420gtgagttgtc tcatggtgtg ccaggatgaa gcctatgtct cttcactctc cttcccactt 171480gttcctccac caggtttatt tccactttcc ccacagccaa ggctgcaagc ccgtcacaaa 171540aataagtaag taagtaagta aataaataaa taaataaata aataaataaa taaattttat 171600ttatttttaa ttaatttatt attttggttt ttttgagaca gaatttcatg tatccctggc 171660tgaccaggaa ctcacttgta gaccaggcag gcctcaagct ctagagatcc acttgcgtct 171720gcctcccgag tgcaggatta aagatgtgta tccctaagcc gggcaagatg tgtttacttt 171780tatttcccac gtctgggtgt ttctccaata tgtatgtaag tgtgctgtgt gtgtatctgg 171840aactagaaga gaatattgga cccctggaac tggagttggt tgtaaaccat ctaggtgcaa 171900gacacagtgg tcacatgaaa atacacaaga gaataaaacc tgggtggaaa agaggctctg 171960ccccctgcag gtcagtaaga gagaaaagca gggataaata gggagttcgg gaaggaatag 172020aatcagttta agtgggacta aaaggtcact gaaggagtgg taaggcccag gaagaagagc 172080ctgaggaaca gggtgtggag ttcccagtag acatgaaagt cccagtggga cagcatgggc 172140agaaggaact ggaactaaga cacatggtgg ggacagaggg ctcgcctctc tgggatatga 172200agaagcagca ggagcagagg ccctggcaga agtcaggaag ggaagacaga gtcccattga 172260ggcccagccc acaaaggaat gtgttgtcag ggtcagtgga aacaggaagg ggaggcctta 172320ggaaatggga ggggcccctt gctcccagga gtgaggtaat atcctgtgga tttgggtgag 172380aagtgtcaga catgcccaaa gtctatgaaa gcaacatggt cacagaagcc cacatggctg 172440gaaatggagg gacgcatgct accgtgtctt ggagagtcat aagatacaac ccccaggtag 172500tgtaccagaa aagaagttta acaagaagct caggggaagg tggcccaggt cttccaggtt 172560tcctgagtct ggcccaggag gactggagaa aactgatgga tagtctcctt ttacattgca 172620ccctctgtgt atctcttagt actcaagtca acactccaaa gcagaggttt ctctctacag 172680agaaaccagg attcaaagag atgtcagctc ttgttcaagg tcttatcctg ggtcaatttc 172740aggcctatgg cctctgatgt ccagttcagt tcactgcctt gtactcagag gctactcaag 172800agctcatctc ctacccaaag cagcagctga aacatctgct cttctcagcg ggagaagcca 172860gtgctcaggc acgaagccag ttactttccc gttgtcccac gatgttgatg attcagggac 172920aaggctgtta gagacacatg ttgactaatg tgaatacagg aaaagaaacc cagggccagg 172980cagaggctag ccagtctcaa gtcaaggtgc agctaccaag tcaccctgag actgggactg 173040ccctcttgtc ccctcctcag tctccctctt ccctcaaact cctcacagtc agaagtaaat 173100ctagctacag ctaggacacc aagttcccag acctccaaca tgcctctatg acctgtctgc 173160cctctgagac catggtccac agagactgtg aagttacctc agcctggtgt ttatgccact 173220gacgcccgtt gatggagcaa tgctaggtgg ggccagtgac gtcgactgat accacaagat 173280gtacccagca tgatgaataa acaccgtgct ggtgtgccag acaagataaa tgttattatt 173340tgtgccactt gtcttgagag agacccctgt gcaccatcac aggaaatgaa tactgagaga 173400gatgtcggtg gagaccaggg caagaggcag agagaggaga accttagggc cagagatgat 173460gacaagacaa cagaagatca tgcgctggta aaaaaaaaaa aaaaaaaaaa accaatcaca 173520gagtgagtaa gaggtggcaa aggtgtagaa cccgaccttc accctggccc cgggtttctc 173580agaatcctgg gaaggggatg gttcatccag ttacataact agcatagcca gaaaggagcc 173640atgccagatt cgcagaagag ggtttcacca catcgcatga aagaaatggc ccagagggtg 173700tttgtttgtt ttttatttat ttggatgaga tgtcgccatg tagccctggc tgacctggga 173760ctcgctgtgt tgaccaggct ggcttcaaac tcacagggat ctccttgcct ctgcttcctg 173820ggtgcgggga ataaagccag gagccaccac tatgcatggc ccattactat ttgtaagaca 173880aggtttcaca atgttgccca agttggcctc aaactcttgg attgaagaga tccccactcc 173940cacccctgta tagagggagg gtcttgagtc atgcagctgg ggatggagct cagtggatag 174000tgtttgcctg ccacgcaggc cgtcctggct tcaatacaat gcactgccat gaatggccac 174060tggctcgggg catagagcca gtccaggatg ttcaagcttg ggttcagtcc tcaagatacc 174120gagcagcaga aacaaaccac gaacccaaac aaacaataag aacaaactga agcagcctca 174180tggtggcctg actgatccat aggtagaaat aaacaggcct tttgagtata agatcttttt 174240tttaaaagat ttatttattt attatatgta agtacactgt agctatcttc agacactcca 174300gaagaggcat cagatttcgt tacagatggt tgtgagccac catgtggttg ctgggatttg 174360aactcgggac cttcagaaga gcagtcagcg ctcttaacca ctgagctatc tcgccagccc 174420tgagtataag atcttatgtg ttaaaatatt aaaaatgcat aaattaacca aaagctttat 174480atttgggtgc ctctgtgtct gtctgtctga ctggctctct ctctctctct ctcgtctccc 174540tctctctctt tctttgagac agggtttctg tgtagctctc ataagctatg tagaactcca 174600ctcctagtga ttcctctgcc tctgcccctt tccgagttca tcttataaaa gaacacaata 174660cagaaaatgt gaagatacac ataagcaaat agaagaccgt cactcataat tatactatca 174720gcaattatat attcttctat attatttatc taggaatatg ttcacataaa catttttatc 174780atagtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgaa gagccagaga gagagagaga 174840gagagagaga gagagagaga gaatgtgtgt gtagatgcag gtagaggctg acatcaggac 174900agatgtgtct taactagcct ccaccttatt ttttgaagta gtagctctca ctgaacctgc 174960ttgattcaac ttggcttcca gacaacgagc ctcagaaccc agagctgggg ttccagtgtg 175020tggctactcc tggcttttcc atgacttctg ggactccagg ttcaggtatt tgtgcttgtg 175080aggccgacac ttttcctact aagccatctc cccatcccgg tataactact ttttatttta 175140gtgtacacac acgtacacac acgtacacac acacacacac actagaggac aacttgtgtg 175200aatcatctct ctccttccat catttccatc ttgtgggttc tgtggatcca actcagatca 175260gcaggcttgg cagcaagagc ctttatctgc agagccactt gctggtccgc attgtggctc 175320ttgatggatg agaatgtggg catactgtga tttatccggc atgctcctct tactgcattg 175380tttctaagtt ttagcgttac taatagggtt agagagagaa caattgtaac aagggattct 175440gagccctctt ttggcttctg ttgggtcctt gcagtcatgg ggtacactca caacacagtc 175500acatacatac ttataaaaac tttaaaaata ataaaattaa cgtataaata agattataat 175560gaacattctt agtgagatat atgtgcttca tgattatttt cctcagattg aaaggggaag 175620tggaagaaca ctgtgctgga caagctagat cctctccatg aatcctttgc cgcaagtacc 175680ccagccctcc caggcgcctg gcaccagcgg cctccatact aaaggagaga tgcagagcag 175740agcctctggc agtctgtcta ggccacttct gtagacaagt gtgcagaaat ggctaactga 175800ggacaatacc tgctctcact tggggctgag ctctcctaaa aacagaggag aagccaggcg 175860atggtgcaca catctttaat cccagcactc gggaggcaga ggcaggtgga tttctgagtt 175920tgaggccagc ctggtctaca gagtgagttc caggacagcc agggctacac agagaaaccc 175980tgtcttggaa aaccaaaaca aacaaacaaa caaacaaaaa aaagactcag ccatatcaaa 176040ctcaccagcc ttcctggtaa gcgctttgcc agccgtctcc tcagcctgat cttgccaatt 176100ctgtagatct gtcaggaggc tgcaagcaga agcgctcgtc atgtaaaggt tgggctgagc 176160ctggggatat

ggtgacatgg tctgcttcta aggcagctca tgcatgtggc tggcaagttt 176220ggggcagagt ttccattcat ctctttaggg aactctccat aggactgctg gaatatcctc 176280agacatcatg gctatcttcc tgactgcagt ggttccagag aaagagtcag ggagccgcaa 176340tgccctctac acccctcctt tgggcacata cagtctgtct tccacaacag ctccttgatt 176400acacagacca cccaaactgt gaataccggt atggaggagt gcagggacca tcagtgaatg 176460aaggtgatta agcagtgtaa ccccacccac ccccacacac accaagaaat gactaactcc 176520ttactacttc atccaaatga acatgcacat aagacttgac gagaaaagat gtgctaattc 176580cagtcattat tgcttgaaca aatgtttatt gagcacctac tagtggaggc tccaattaag 176640ggaccagtct acagttaaga tcagggacac aaggcagtac cctccagccc tacatgggtg 176700tgacagttct aagtcccaga tctttaatcc ctagagcact agcccaccat atcagaacct 176760ccttcccatg ctcggaaatt ccagactctc taatgtgctt ctctgctctc agctgctcgc 176820catccttgcc aagaccacca ggaccccact ctatttgttc tcagacatag acatccccag 176880agaacctcag gctagtttct ttctctgaag agcaggcttg gacttccagt gccaacagca 176940gaaggccagg atgcccccag gcaggagctg cttttgaccc tgccagggaa atctctcaaa 177000agcccactga cctgaagaga aggtgggcga catgctagtc catcagtcct accttggcta 177060gggcagccct gtggggaagc accaggatac tgcttctttc agattctaga agtcacaggc 177120ctgtgaggag gggaaggaca accagggact caaggtcgtc cttagctaca cggtgagtgt 177180gaagccatcc tagatcctgt gagactgtag agagcccggg gacactgctg ggaagaaggc 177240gagttgaagt ggctactgtg aaacctatgg ttcttccttc cttaaaacta agcagagagg 177300gaggagagac agcccagtgg gtaagagcac tggttactct tttagaggac ccacgttcga 177360ttcccagcac ctacgggata ggtcacaatg gtctggaact ctgtttcatg catgtggtgc 177420acagacatac atgcagacaa atgtttatac atttaaaata attataatta attaattaat 177480taattaatta aaaaccagcc acggagagcc tggagatgtg gtcagtggtt acgaatgctg 177540gctgcacagt catgaggtct gcagttcggt ttttacttgg gagccagaga caggcggatt 177600tctgagtttg aggctagcct ggtctacaaa gtgagtttca gaacagccag agctatacaa 177660agaaacactg tctcgaaaca aacaatacac acacacacac acacacacac acacacacac 177720acacacacac acacacacac acacacatac tgtagctgtc ttcagacata ccagaagagg 177780tcatcggatc ccattacaga tggttgtgag ccaccatgtg gttcctggga attgaactca 177840ggacctctgg aagaacagtc agtgctctta accactgagc catctctcca gcccagagtt 177900caaatcttag cactcaagta acaatctggg tgtccaccta ggctaatgtc tccagctcca 177960gtggggtgaa gacaggagga tccctggggc ttgctgtctt tcagaacagt tgagaaaatg 178020ggaggccagc ttcaggaagt aaccctccct caacagaaaa ggtggccagt aacagaggag 178080gaacaacagg ccctcttctg gtctccatga aggcacacag gtgacatagc tgcaccctat 178140gtgcgtgcac acacagagag aagttgacga atagaccttt ttaaaaagtt aagcatagaa 178200ttaccacatg acccaccagt gtctcctcca agatacacag tcggaagcac aaggagcagg 178260gactgggatg gctctgagtg cagctgagtt catcacagta ttcacaatgg ctgaatgagg 178320atgtgttttc tgtctggggt gtgttgctac aattccttcc ttgggaaaaa cataaatact 178380actcctcctc ttgaggtcca gtgactccaa gttcactgtg gaaacaatca gtttcctggg 178440tttatttaca aagcaatgtg agaggagtgc cagcagaatc ataggtgact ataaaatagc 178500catgctgaaa tgtcttcacc cagcagggat agtgatttcc ccatggctgt gtagaaggaa 178560ctcctaccct taaccgtacc atctctactg gctagctact cttcaataca acatgcagct 178620atgcagtgat ggtacactcc tgtaaccctt acacttgcga agcagaagca ggcagatctt 178680tgagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 178740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncttgct tcttncatcc 178800acctcntttc ttccttcttt cttccttctt ccttttttct ctttatgaaa tagggtctta 178860ttctgtactg tagtccagac tggcttgaac ttgctgtgta gatggtgcag gggccttgaa 178920cttgtagcag tcctcctggc tgtgcctcta catgctaaga ttgtaagtgt acaccactgt 178980ctctagctaa gatttcttgg aaatcttgat agaattgtta tataagccag cactcgggag 179040gcagagaggc agacaaatct ctgggtttgt ggccagccta gcctggtcta cagagtgagt 179100tctaggccaa caggaacagg atggcctata cagagaagcc tgtcgaagga agaaaggaag 179160gaaggaagag gaaggaaggg aagaaggaag gaaggggggg ggagagggga aagggggaga 179220aactgggcaa agggaagcaa gtcaccttca tttcttacat accagttgca ccttcatgct 179280taaacagtga tcatcattct cttctcagtg tttttctcat ccacagacta atctccaggt 179340cattgaggac atgtctgtct tgtccctatt gttctgagag cactttggtg gagcatcaga 179400cattagaagt acttttctat taccacatac atctctcagg gttacagctg taatgtctct 179460ggtcaggtga attatgttaa tctcttagct ttgacctaag aaggcatttt tttcacttgc 179520ttataactcc gaggctatac ctttgagctc tctaaatgcc actcccacct tccgtattgg 179580gtcatgtctg aattatattt atcattgtgt gtgtccacga tgaatgtgtg tcactgtgca 179640tgccaacggg cacctgtaga ggtcagagga caactctgtt gagtcggttt tttccttcca 179700ccttcacatg gtttctaggg accaaactca agttaccaaa cttgctccat cacccactga 179760gagccttctc cctggcttca tagtgtttgt ttgtttgttt ggttggttgg ttggttggtt 179820ggttggttgg ttggttggtt gattggttgg ctggctgtgg ttgctgttgt ggtggtggtg 179880ttggtggtgg tgattttgag acaggatctc actgagtagc agtggctgtg ctccaatata 179940cagagagcca cctgcttctg tatcctgaat gctaagatta aaggcatgtg ccatcatacc 180000tggcccttga tttttaattt gaaaaatact gtatttaaat actgggtttt gggctggtga 180060gatggctcag tgggtaagag tacccgattg ctcttccaaa ggtccagtgt tcaagtccca 180120gcaaccacat ggtggctcac aaccatccgt aacaagatct gacgccctct tctggtgtgt 180180ctgaagacag ctacagtgta cttaaatata ataaataaat aaatctttaa aaaaaaatac 180240tgggtttcca tttggtatca ttaggttctg tttaaaaatg aaagaggtct ttattcattg 180300tgctctataa aaggtgtact aaaacttctg agtaatatgc tcacctgcaa gtattgggaa 180360cacactgagt ggacctttgt ggctctaatt tgttgtgact tagaattctg atgatggcat 180420gcatgttact attcctaaca gtttcggtgg gtattgtcta catgtcttat agaaactgtc 180480tgatgacagc accttttaaa atggactcat ttaaacaagg agacttctgg tagggctttt 180540caattttgct ttcttatggt aagagtgggg ttgccaggca gtggtggcac atgcctttaa 180600tcccagcact tgagaggcag aggcaggccg atttctgagt tcaaggccag cctggtctac 180660agagtgagtt ccaggacagc cagggctaca cagagaaatc ctgtctcgaa aacaaaacaa 180720acaaaaaaaa agaagaagag ttgggtagta gtatctgcag tagtgttgag attggccaat 180780ttggtgatcc cacctgctag tgcatgttgg gaaaacccca taagttcttc atcataaatg 180840ccatttcccc atgaagggat tttccctgaa tgtgtgtttc aggccgtgag tctctcatta 180900cttctttgtt agcttctagt cagtaagttc agccaggggt tgatgcaaat gctaaacact 180960aaggattccc ctaacttttc ttcaccgtga actcattgat tcctggaaga tttcaaaatc 181020acctcatcag tgctgtgtgt gatcctacga tccacactgc cttctgttgt tttattcatt 181080aaagaagtaa taatagcaac caatgcttag tgaatagtct tgtaggcgac ttaaagaact 181140agttctcttt ctttgctgta tgtcatatta gcagcctggg taacaacccg attcttattt 181200atataacttt ttccactcat tttggtcttt attatttttg agtcatctct aacaagcccc 181260ctttctctgt aagtccatgg agatctcaga caaggaccag agttaactgt gcgttggggt 181320gtttgtctct ggttgtaagt tgtcctcaga agattacagc tttgacagaa tgagggatct 181380tgtctcagtg cttcctggtg cagatggaca cttggaaata cggatatatt tccaatttat 181440tgtcctccca agctatactc tccatatata tatatcttgt tgagtctggt agacctctta 181500aatctttccc tctgagtgtg tatatataca tgtgtgtgcg tgcacacaca cacacacaca 181560cacacacaca cacacacaca cgcacgcagg aagggaagtc tagctctcca gaaggcttgg 181620ctgtcagctg cattgctttg ggagcaccga gaacactgac actagctgaa ggctctgccc 181680cgtctgctca catctgcttt ggattgttcc ttggtcaacc aggaaagatg tccttgttat 181740tgctctgaag tggtggactg aacaagaaaa tttgctcttg gaattgtccc gatttttaaa 181800ttagtgccac cagccctcct ctcttttccc accatgtttc acatacccag ctaatgatac 181860gcttatttta ggatacagta tcactacctt atatagcctc aggcgcctca tctcgcctag 181920aacaaagagt ttcaaggctc aaaatcaatc ttggcctctg gtatttaaac catcctaggc 181980gatgaacata gggttcaaat taggcctctg ctttgtatta agagaaaagg atgtaaattg 182040ccttttgagc ctgcagacaa atgctaacta accggggtgt tgggggttgg aaaagacact 182100taacaaatta agttgagttg cttaggagtg gagaaataaa tgaccatagg ctccacccat 182160atggcttctg ctgtgttggg tgtaactgct gtatccagtg taaccaattt cctttgtgtg 182220tgccaccaca cgtgtgtatg ttcatgtgtg tacaagtggg catgtatctg tgtgtatatg 182280taagccaacg gatttcactg ggaattgttt aagtgttatc cttctagcta cctatgaaca 182340cagggtctct tactggagcc tgttaccccc tgattaatcg gggatgcctg gccagcacac 182400ttccacaccc gcctccttct ccccagcacc ggatttagaa acatttgtca ttacatttag 182460ctttggggaa tcttaaactc aggtcccctt gcttgcatcg caagcatttc ctgaatgagc 182520catctctcta gcccttagtg gtctctttct gttatgactt tgatttcttt tgtttgtttg 182580tttttaaaga tttattatct atctatttat ttttatatga gtatactgca gccgtcttca 182640gacacacact agaagagggc atcagatccc attacagatg gttgtgagcc accaagtggt 182700tgctaggaat tgaactcagt acctctggaa gagcagtcag tgttcttaac cactgagcca 182760tctctgcagc ccacgacttt gatttcttag gtaaaaatct ttatgacatt tatcacctct 182820ggttgagtct gtaggccata aatcttttcc tatcctctga aaagggactg ctggctgtct 182880ccatctatta ttcaaggcga tgctttaagc tggacatcca gtgctcacct gtaatctcaa 182940catttgggag cccgaggcag aagaattgaa aattctccaa cctggtctca atctcccacc 183000ccaaaagtaa tattttaaca tctgtatatt tgaaagagca tagtgaattg taggttcatg 183060tatgcttacc cttggaagtg taaatgaaag atttctaaac attgcagata taaccttccc 183120tctaagatac tggtcattct gaaatcattt cttcccgggg cttgttttct gtatacagtc 183180agcatatcta caattatgct tttcccttta tgtcacctca agtatccgat ccaggatgct 183240ctgtaaaatc accgtgtgcc atgttgcatg caaagcaatg tacaagtaca ggtaggaaga 183300atctttgatc caccctgaat tactgcatga atatccaaat tgctcttcca tatccaaagc 183360ccccttcatg acgttggctt cagcgctgca cacctcccgc tttctcgggc ttttaggatg 183420ccctgtaact ccaagtattt acttgccctt gtgagctcat cagatcccat ttgatccgag 183480tctttcctct gcttccgttt gtatgtgact tgtatggttt cactgtggtg acattgcacc 183540agagtttagt ctatggcatt ctccatcctg ggccacatat gcctttcagc cataatagca 183600gagataagct aaggtcactg tctagtgagc ccagccgcag agcgacacgt cctggcactc 183660caggttttcc aggtggtact ggttctgggg acgcagggac cttttctagc acagaacatg 183720tacttttgca gaagtggctg ggctatgctt ctgctaactc cagggatatc atgcatgact 183780taaaaagtat agtcggaaaa atgtttttaa aagttctgcc aaaatctcat ccacttttag 183840ttatctaatt ttttaaaaac tctatcctga gttttctttt ttcttctcgc tgtccatttg 183900acttgaccct taagctagac atgctgaaag aaggatgcca agtccatagt gacagacagt 183960ggggagtgga tggacgcctg gtgctgggat ggtgcttagt acagggaggg cttctttctg 184020tggtaaagta ctctaaatcc accctggtta cccaattctg aacgtaccaa aagccactgt 184080cgatgcccct taattagatg aaattataaa gtgtattata aagagtggat ctctgctgag 184140catgtatcaa gaagcacagg atggtctcat cttgtcttat agaacttctc aaagccgggc 184200agggagggga gggaagcaga ggcccatagc acccagcatt tagagctgag ctcatttcac 184260ctgatgcagc tgtgcattct gggtagtgcc acaagcccca cgcaacaccc agggcagtcc 184320cttcatagtc tcttgtattc acttagcaag caagttctga gtgctctgtg cttgatcatg 184380ttcccctcca cccccattca gtttccagtc cacttttctc tctttacctt cccagatcac 184440ctaagtgtga tcttgggttt gttttaggtt ttgtgtgtat gggtgtgtgg tgtatgtatg 184500atatctacat gtgcacattt gcacctgcac taggaggcca tcagatgtcc tgctctatcc 184560ctctccattc ttttgtttga ttggtttttc aagaaagggt ttctctgtat agccctggct 184620gtcctggaac taactcgatg tgccactact gcctggctcc ctctccatac tctttctctt 184680tctctctctc tctctcaaga tttatttatt tatatgtata ttattaagat ttatttattt 184740tatgtagctg tcgtagacac accagaagag ggctcttaac ctctgagcca tctctccaga 184800cacacacaca cacacacaca cacacacaca cacccattct cttcagacag ggtttctcac 184860tgaccctgca gccgacctgg caaccagcaa accttagatt tccccctcct cagcaaatgc 184920cggttggcta cactcagcct tttagtgagt gcttcagatt tgaactcagg cttgcacagg 184980aagcaggctt catgctgagt catctctcta gccccctgct tcctttttga gtcatccctg 185040agatggtgtc ccttctgtag ggattgtcat actgcagtat cccctgcaag tcctccagac 185100actgatgttg aatgagtgtg tgaagagatg gaggcattca tttagagtcc tgtatgaggt 185160gctgggagta tgcagcagta ggcagtgtga gtagcagaaa cagcagctcc tgagcccatg 185220tgcctttgga agagaaatca taacttttta ggtacaaagt aactgatcaa accctggctc 185280tgtgccttgt tgttgttgct gttgttgtaa atcttgttga tatttctgac ccaagccagt 185340gagaatccat ccttgtttta gtctttgctt tatttttgtt agagtttttc ctcctgtgtt 185400ggttgatatt tagtttattt cagttgtctg tcctataaac agtaaaggct gctcacccac 185460gtcttcccgc ccctgcttca accacacatc ccagggtccc cctgctactt cactaaactg 185520ccaacagttc tgaaaaatag ccctctgctt gaagtgtgca gaagctgccc acaacggtaa 185580gcagctaaga gaaaccagag cacatatttc ctccaactca ttttttttcc agcccctcaa 185640gtatttgtta gagggagctg agttttcaaa atgagttcca atgttcccgt gtgttaataa 185700tttgaggttt ctcctgcatg gataatttaa atgttcaaaa ccaactcatg ctaaagttaa 185760atgcacattg aactagccgt actcctagct taccatatac atggcagtga tggagaggga 185820tgtgaggtaa agagccactg gcagggaaga tggccacaca gtgctaagga aacctcgctt 185880tacagtgatg cattgtcagg aaggatgctg ggcctgcccc tgctaggctt ggaaacttaa 185940actgcgttta aatcatcttt cacagcacca ccacaagtgt cccactgcag attcttgtta 186000gtgatgccag cgagccctcc gcactgagct gcctgggctg acctcaaact tcgagtcctc 186060ctgacttagc ctcacctagc cggggttaca ggcttgtgcc atcaagtcta gggtctgtta 186120ggatttttaa caacctatcc tacttgctta tgaagagttt ggacaatttt gctcactgtg 186180tgcagactgt gaggtgtaat tggacattga aaagattggt ggtatattct tctagtccta 186240gcactcagaa agtcaaggca agaggattct gagttagaga ccagcctgaa ctacaagatt 186300tccttcagct taggctgcct gtgtggccat agggagactc tccagcaagc catcaattgg 186360tatggcttcc agctgatcca tgggcatggc ttccaatgtg ttctccttca gaagcagcat 186420ctgctgtttg tttttgagac atgatctgac tgtgaagtcc tggatggcct ggaacccata 186480atggagacca agctgacctc aaatccacag ataaccaatc ttcctctgcc tccagaatgc 186540tgggattaaa ggcatgagtt accatgccgg tccataagca ctatttctta cacactcact 186600gtgctcgaag aaatgctaag tatcatgaaa gccaggaaag aatagtagcc ttttttaaac 186660atgaatgcta ctctcccacc ctcccatctc ttcggcccct ttcatgtccc cccactcctt 186720ctcaacttat ctcctctttg taccggtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgagag 186780agagagagag agagagagag agagagagag agagttactt ctgaccactt gggattggat 186840aacccttggg gcctcacctc tgtcgaagag aagactgact ctccctctct tggtagcttt 186900aattacatgc agctcttcat ctagaggtgg ggccttgtga gattccctcc atccatgcat 186960gatggcgtgt cagctgtgtg tgttgtcatt ggacaggtct ccttcagcaa ctctaataag 187020tagttctaca gaaactacgg agaaagaata gacttctcta agagaggtag agtgttttta 187080aagctgaaag aattacacac acaagataaa gagtcacagg ggagtacaga gaaaggggga 187140gggggattgt cttagtcagt actctatttc tgtgaagaca ccatgaccac agcacctctt 187200atgaaggaaa acatcttgtt ggctggcttc agaggtttag tccattagcc ttagggcagg 187260aagcatggca gagcagaggc agacatgctg ctggagaaag agctgagagt tttacatctt 187320gatccacagg cagcaggaag ttacagtgac tttgacttct gagacctcaa agtctcaccc 187380cctagtaatg cactttctcc agcaaaacca cacccatgcc aacgaggcca cacctcctaa 187440ttgcatcact ccctatgggc ctatgcaggc agtttttact caaaccacca cagtgatcta 187500agagacttca caacagggtg tttatttctg ttgacaaatg gcactgtttt ggctgtatca 187560gaccctgaac taaacccctg gtgatcagta agctactagc ttcagcagct tgggtgagag 187620aggttggaga tagaggttct ggtaggtggc cattgacgca gcagtgtgag aaggcaccag 187680tcagtgttca ggagcgactg acacagtaat ttaactggct catttttgtg aacctttcta 187740tcgtatatca aagattctgt gaaaagatga gaacaaataa acctctttcc tctccactca 187800gtagtcagta gaaagcttga acacatcata tagctaagaa caatgtaagg cagagtggag 187860actgggatgt cagagaataa acaaaaggct ctgacaacca aaggaagcaa ctgatatggt 187920attttctgct ggaaatcagt taaagcttca tacaggaagt aatatttgac tgcaccttga 187980aaacggatga cattttaaag gtgggagaga caggaacagg cctggcacag acaggaagta 188040tataaagaaa ggcatataaa cacaggatgt gggccctcta gtactgaaga ttagccaggg 188100gtactcagga gagctgggta gggtggggct ggctgtgatg gaacttaatg acaatgctaa 188160aatagagagc tgcctctttt tttttttttg agcaaagaga taacaggatc tgattttgta 188220tggcagccat tgagctctga gtgaatagct taggaggtgt aaaaggtaag attctaagca 188280agaggggctg gagagatggc ttagcagtcc agaacacttg ctgcttcatt gtgaggacca 188340gagttcaaat ccctgcacct atgtgagaca gctctcaaag aggctgtaat gccaagaaat 188400ccagtttcct ccgctggcct ttatgggcac tcatacaaac ttgggcatac acccacatgt 188460aaaagtattt ttttatcccc tgatgtaaat gttgcttcgg aggctattgc ctctgtctgc 188520taacctagtc ctggaagctt ctagcctcag tacgacctaa tctaggccta gagtgttttc 188580agcccctgag acttgctgct gaataagctc accctttcta gttctttctg agctgtagcg 188640caaacactct ccaagctgac tgattcaatc tggctccccc gccccctccc tccctctcct 188700gagagttagg catatcctgt tctgtcaaat cattctctga ttcatcactg tgtcactttg 188760ccactcaatt tgacatcact ttccacatgg gcacttcctt ccacaaacta aacttaacct 188820tcattgtttg agattaaagg tgtatactaa gggtgtgtct gtgtccatcc aggtagatta 188880aaggtatgtg atgagactga gccacaccac aactagaaat aggattttcc agtaaataac 188940acaatcgcag agttcacagt gtgatcagat atcctgcaac acccataccc ccacacacat 189000gatttttgtt ttaagttgac agatggaaac aacatgggaa caaagccagt gtattttctt 189060ttaagatttt atttctttat tttatatata tgataagtac actgcagctg tcttcccaca 189120cacctaaaga gggcatcaga tcccattacg gatggttgcg agccaccatg tagttgctgg 189180gaattgaact caggacctct ggaagagcag tcagtgctct taacctctga gccatttctc 189240cagcccccaa agccagtgtc ttttgtaaca gagcatgcaa agatagtctt aaccagcagg 189300tcctccgtgg gagaattctc tggctagtta gagaaatgca cagggcagaa ggagaagggc 189360aaaagagttg gagaagggaa aggggctcca gggaggtagg tatgtcgcct taagattgct 189420gcagggaatg gaaaaggcag ggagagtgcc aggagttcag ggccagccag ggaggctggg 189480gtgtccttgg ggtagagtcc atcaattaag aagttttcca gcatgggtct cccctccttc 189540acctttcctc tgtccctcct ctcccttttc caggtgcaca tgaacaaggc aggtgaccca 189600gtcccagcga gctcagatgg ttcactgcct acttcctaga tttgctcttt cagcctggtg 189660acacagctct gtgttccgct aaccagagac cagtgcctgc tgggatgctg acatgagtgt 189720tgttcccttg cttctctaga ctttggcttc agcaacctct tcactccagg gcagctgctg 189780aagacgtggt gtggcagccc tccctatgcc gccccagagc tcttcgaagg gaaggaatat 189840gatgggccca aagtggacat atgggtgagt atgtcccgtc ttatcacctg tcccctctgc 189900agatgacagc tgtcctgtca tgtgggagtg cagagcacgt tcacctgcac ttgcaacccc 189960agtctccccc agcacccaag tcacatgagc cttggctttc aactactccc tttgcacctg 190020cagaggaccc ctgtgagggc ctgagcagaa gatggctgtg gcaacaacag agattacctt 190080ttcttgagag gctgggtctg agaaatgttt ggaatctcac cctaaattca tgtctggaag 190140cagaaatgtt acctgctctg aagacaattg actttgacac tgacccatat tatatcctaa 190200aaagaggaca agttggtccc atcatctgca catacactaa tggtctcttt tgtttcctcc 190260aatgctatta accgtccgta ctctctgttt gggctacaga gccttggagt tgtcctctat 190320gtgctggtgt gtggcgccct gccgtttgat gggagcacac tgcagaatct gcgggcccgc 190380gtgctgagtg gcaagttccg catcccgttc tttatgtcca caggtaaggg acccgccagc 190440ataaagccat gactgcattc acggagaggc aggcatgtgg agtaatttca caagtgcagt 190500gatgctgggt cagtggccat ttggctgctc tctaagctgc agctgcctca gctcgtccag 190560gaggcaaggt tccttaggag cgaaataagc cacctgcttt ggcttgggga tcgggctctg 190620ctcagtatca attcatctga gctgttgtac ctcagtctaa gacggctaag gctttgcacg 190680gcccctggca tacagattag attaactgct gtgtgagtca ctggtgccat catggtgcta 190740cagtggcatc agtgcgtgtc acctgtcctc cccacagcct ccactggagt gccagagctc 190800cgcggttggc actgatgccc aggacaaagc aggctttcgt ctttgtcttc ctatggtctc 190860atttcctagt tctctagttc tccttcagat tctaactatt taggaaaatt ttttaagtaa 190920gcagacaata ggataatagc tttttggaaa ttttaacact gaaaacggat accagaaacc 190980cagatgttat agttagcaga caactcagaa cctctgggtt tggcttcagt ttgggatgtg 191040tttagttggt tggtttggga tttctttgtc accatttctg tgcatgtctg atccactata 191100gacagtgtta ccttttgtct agctactttc ctcttaagga gagcatgcac acctttagaa 191160tatgtaggtg gagtgtgggt tccgtttaac tgagtcagct cagaactgat gacttagaag 191220gtcatgtgtc

aggtgatgat aatgctaagg aaaggggaag aagaaacctt tagaatggtg 191280ggtgggagat cgttcaaaga ggaacagttg gaaaacgtga catttcaggg acaactggaa 191340acgaaggata ctggaggatt actgcccgaa ggccccaggg agggagcaat tggggtgtgc 191400ctgaaaggag ttgagcatgt agactctctg cttttactct gctggaatcg gaagaattag 191460aagctctctt aaaaatagag aataatatgg ttagacctgt gggggttttc taaattttta 191520ttacgaaaac tttttaaaaa ttgaaaagat ttttttcatc agcttttaaa gacaatccct 191580gtagttgcta cactgcttgt tgaaaggaga gtgcagtgca gtgctgtgtg gtgcagtgca 191640gagctatgtg gagtagtgca gtggcatgta ctatcacgca acacagtgca gtgctgtgtg 191700gtgctgtgtt ctaaggcaca gcacagtgta gtgcagtgtt gtgttgtgga atgctgggca 191760gtgcagctca gtgtaatgga gtgttgtgca gtgcagctca gtgaagaagc ctggttggta 191820ggagacttag agagcatgtt cccagactga ggaatagagt gatttgggac ggatcgctgc 191880tgcacactac ctggttgatg tgtcagagga attttacaga cattagctcc cttcagtatt 191940atgagctata tgaggttgtt accccacttt gcaggtggtg ccacatccag gagagtgtaa 192000gcagtgtccc catgtagaac agcttattag tggctaaact agaatttgag ccaaggcagt 192060atgtctcaaa taatgtttga tattgcattt aaggtaagaa agaatagtta caggtaaccc 192120cgagatttgt acctgagcaa cagaaaggat gaatttgcta ctaaatcaag gataactgaa 192180tcgtcaacat tttggagaga tgtttttaga aatgtcaagt ttgtggtgcc tattagccgt 192240ctaaaagact gttctggtca gcagttggaa aggagagagt tgtaactgtc aaaactggcc 192300tctttgagtg tcatagaata tactctaatg ctaccaggaa ggctcgctct ctgtgcctct 192360caggatggtt agagatgagg aaggtgacaa gggccagaag aatggttagg gaggaaagcc 192420agagtcactt gaagacaagt gaagctgccc caggaagctg aggtagagga cagagaactg 192480agtgctggat ttagaggaat ggagctccct ggtggggttt ggtgccactg ccacgggaag 192540ctgatccatg cacacagtag gaccgagtgc aaggcacagg aaagggggaa gccttgactc 192600ttcccagttt gactgtacag tgatggagac agagaagtgt gctctggggg gctccaaccc 192660tggtcatcag atgtcctgcc agtagctgca tttcctgttg tcatggtgac tgcattttac 192720cattccctac taaggaaaaa aatgaggaca gttttaggtt cactgcagaa ttaaagggac 192780agaagatgtt catatgcccc ctgcctcact tcccctccca cacacataca cacacacata 192840tgcacacgca cgcacgcaca cacacacaca cacacacaca cacacacccc ttcattcaga 192900gcctgtcatt tacaagaggg ttcactgtca gggttgtaaa ttcgctgggc tctgacaagt 192960gtataaaggc atttatccac catgatcctg tcagacagag agccccacct ctgggatgat 193020cctgtgtgct ccccccattc ctcccatctt tctctcccca ctggtgatca tcgtctccat 193080acatttttac ttccgggatg tgatatggtt ggaagtgccg ttttacgttg gccttccact 193140taggaacatg cacaggcatg caatgtctct tgtgtcttgc tagctcattt ccctttacta 193200ctgagtactc ccttgcctga atgtactaca gtttatcctt ttgcccactg atgagtgtca 193260tggctgctct ccagttttga cagtttgtaa ctgagattgc tttgatagcc ttgtgcggat 193320ttgtgtatgg acacacgttt tcggctcact tgagtaagtg ccacggagca tgcctgtgag 193380gccatatggt aagagcttgt ttagatttgt agtagactgc caaacggcct tccctagagc 193440tgggctgtgt tgttgtaaat aagagttctt actgctgccc gacttgcggc ttttgcctga 193500ttagcctgtg gggcgtggtc ccctttcaca tgtgtgtggt gttcctttca tgtgtagttc 193560tgaagtctgt gatcctgaac atccttgtat aggtgtctca tcaggtcttc attttttatt 193620cctctccaac aaaatctcat agtgtttttc caagctgcct ttgaactcct gggcttacac 193680agtcctgcct cagtctcctg aggatctgga attgctcgtt tatttattag ggtttttcac 193740tgtcttctaa accccattgt ttcttactgc ttctgagatc cattccttct gtcccctaag 193800tatactctta gtcatctctg ccagattttt cgttctctct taagccttac atctcctctt 193860acccaaatga gctgtctgag ccatctcgtt tactgccatg gcttcaccga tcccttttgg 193920ggactgctcc caattaactg tctccaaatt ctctcctgag gtctagggcc acaaatatcc 193980atccatttgc tatttagcat ctttaaaaaa attattttcc ttgttttgta gtactgagac 194040tcgaacccag aaccttgagt atgttaggtg ggcaccctgc ccctgaacta caacctgtta 194100ctcagtgtct tcctttaaca tcttgaaatc acattcactg aagcattttc caaaatgaac 194160tttattccct atccacagtt ttgctctatt tggtgtccct ggtgtccccg tctgaggctt 194220ggcagaaaca agtcttgatt gtttcttctc ttatcaccac cagagccaac tggttagtaa 194280aggctgtgca gtcagtctgc tattccagta accatgctgt catcttctct tgcctaagtt 194340agactttgtc aaatctgtgg atgctgagtt ccatccttgt cttctccatg tttctgcgtg 194400accttggcag acacgaataa taaaaactac acctcacatg caaacctcac aggtacacgc 194460atacaaacgc acaaatctga tgatatcatt ttatagttta aaaatgtttc agcagtttaa 194520cattgcccta aagtttactg ctaatgtgtc taaagcactg aaattcaaga atttttacta 194580cagccatgat aaaaaaacat actttatact ataacttaga acacacacat atatgttttc 194640aaaaatcaaa atatattgtt ttgagacaga gtctctttct ataacccagg ctgattttaa 194700actaaagaac catctcgccc agtctcctga gtgctggagt tgctggcagt ctagcctgat 194760cgagtgctaa gccgatgatc ctgagggtga tggtcaagat tgttctctgg cctacacacg 194820tgcatattca agaaaaggca aattcagata ttttccgggg tttttatctt tctgaacctt 194880gtcttactac tatgacattt atcaaaatat actgtaattg gaggctagag tggctcagca 194940gctcagagca ttgtctgctc ttccagaggt cctgagttca actcccagca accacatgat 195000gggagctgat gccctcttct gacatgcagg tatatatgca aagtcatgta catgagaata 195060aattggtttt tcgagacagg gtttctctgt gtagccctgg ctgttctgga actcactttg 195120tagaccaggc tggcctcaaa ctcagaaatc cacctgcctc tgcctcccga atgctgagac 195180acatatattt tttaatctta atttatttct tattgtaatt gattctttca ttataagcct 195240tccacaccag actctgaact taaagaatat gcagagaaag cctccagcct tcccacagtt 195300gtttataatc tagcagtgtc tggtacagca gcacttccta cacactcttc tgtgtcagag 195360tcaactgcac cgaccttgtg ttccaggggc tttcattgac tcttaaaatc aggctgctgg 195420tggtgcaggc ctttaacccc agcctgggag gcaggtagat ctgttcaagg ccagcctggt 195480ctgcatagtg aattaagacc ttgtctcagc ccggcggtgg tgatgcatgc ctttaatccc 195540agcacttggg cagaggcagg cagatttctg agttcgaggc cagcctggtc tacagagtga 195600gttccaggac agccagggct acacagagaa accctgtctc aacccccccc cctcccaaaa 195660aaagaaaaga ccatgtctcg aaaaagaaaa atgagaaatg tacacaattg tgacttcttc 195720tgtagcacct cacccccgcc ccctccccca gtcctgtttt ctcttgttct gacctcaggc 195780cctgggcttt agtcaggata gtgtttgtcc tttctcagac atagcaagca cactctgctg 195840tcccctcagg tgggcactct gaggcctcta ttgacaccgc cctccccact gccaccaaca 195900gcttccataa ttgtgtttgt cttaaccctt gtctcagctc cacatgctct aaattgtttt 195960cccacctccc atgtcatcat tgtcgttgtt gtcgtcatca ttgtcatcat tagctggcag 196020ggatgagcgg ttttggataa gttttgttca ctgctatatt cccaggccat ggggtagtgt 196080ctggtgtcta aactcataaa gatgtattaa aggagggaac tcatacacag gtttgttaaa 196140aatacagtct acgggggtgg gggtgggtac ggcccaccaa agcccttcca caggaaatac 196200tctcactcaa acaatatcaa gaaattacat tgtgcgtgtc taatctcagc acttgggagg 196260tgaaagcagg aggctcagga actgaaggtc attagctgct cagtgagttt gagtctagcc 196320tgggccacat gagatcctgt ttcagtcctt gctccaccct taaatacaca caaaaataac 196380tacatttctc taaggctaga gtttgtgaag tattttcctg tatttcgtct gctttaaacc 196440ctgcagctgt gctctggaat tgttactata tactattttc ctgcttgccg tgacgtgtag 196500agaagtgagt tgccttgtaa aagccacaca gttcacccca gtcagccact cctcggatgc 196560tgtattgttc tgctcaacct cactgttata aaaaaggggg gggcaggtca ataaaccact 196620tcctctgctt ttccagtgtc cacagtattt cctgtgagga gtttggatat cctccatctt 196680ccaccccagc ctctcagacc atgcaaggct cttcaggcct ccccaaagca ggctgctgag 196740catagctcct ggcaaataac ctagctgctg aaaccacgct cctagtgagt tatcatgagt 196800gctgaaaggc tctgagaatg gcagcgtgat tgtcccaaat agtgatactg tataatcccg 196860gagaggagat tccccaagtg aatgaaacaa tgctctgtaa gatgaagcac agaaagcaag 196920aaccagtgga ggaaaagagc tggcggtcac catcttactg catgctcgtt ctcatcctca 196980gcgatgtgca tgtgcttagc tttatcctgt accatagacc tgaatcccaa gactcatcag 197040aggctggtac tatccatatg gtgaggcaca gaggaaaagg aaatcatagg atattccttc 197100aagcatcatg gagttcagtt tcaagggaag tacacttgag agtctcaccc ggggtcctct 197160ttctgtctct gggcatagct gtgactcact agacctggaa ttagctcttc ctgtggaggt 197220cgaagctgtc aggtctgctc tccctctttg tgcttctgtc tctttcctat agagggcagt 197280gtgggtgaaa aacaagacct gttctctgct gtcaccacct tggcctgagc ttactgattt 197340ctgtgaaaag atgtagaaac tcagtcaggg ctgagagagc tttccacatt agcctgtgaa 197400acactagaag atcctttcct ctatctttta tggccaagtc agactccaga gtgtctccag 197460ctgggagatc agtctctgaa gatggacact gtttctatag taactgtggg atgaagttgt 197520cttctcatag taggtcaaag cttgttagac acgagtgttc ttccatagct gtcctgagat 197580caactaaggt cacatattgt tacatggcat ttgtcatggg ctaatgagtg tcatgagtgg 197640gttttaacta cctagtttat tatggcctaa aacaaccaga tgtgttttac acagctgcat 197700cttctggaac ctagaggcac ctggcaggaa ttacatgtct tttcagtgac aattttgaat 197760gaaatatgga aggaatcaca acgttggtct tatggtggtg gataccactc aggagacaaa 197820ggcaagcaga tcctgtgagg tcaaagctag cctgctctac atagcaagtt ccaggacagc 197880cagggctaca taatagaaac tctgtctcaa tgtcccaacc tcccacccca ccaaaaaaag 197940gaaagttgag aaaggactta ctacttaact gtagactctc ctgtcgcttc ggcattgtac 198000tattaaagtg agaggtagct ccccaccgat ggttggacac actcaggaaa ccaattggct 198060ttataatctc ttgttattat tttgtagatg actagatcaa gtgacttctg gttagtttgc 198120aaaggtattt tgagccagtc atcccggcac tcaggaggct gaagcaggag gaacataagt 198180gagttggaca ccagcattgg ttacatagga ggttggaggc cagacggggt tacataatga 198240gaccatctca cagcgaaaaa caaaacaaaa caaaacaaac tatttgattt actgtaattt 198300ggctctccta gtctctgata aatcatgttt gttacaaacc atgtttgttt tgttttgttt 198360ttaaggttta tttattttta ttatgtataa agtgctctgc ctgtatgtat tcctgcaggc 198420cagaagaggg caccagatct cattatagat ggctatgttg gaaattgaac tcaggacctc 198480catagccatc tctccaggcc cagaccagtg tttttgagtg aagcttcttg gattgaccca 198540gagctcagag ctgtcatcca gggagcattt cagcctttgc cacaagatga taaggggact 198600catttccttg agctcatata aaatgagacc tggtctagcc agtgtcagca gtggccacat 198660ctgactccac ccactgatgg aagccagcaa tatacacatt tggttactag attcactccc 198720tgataataca tttttaaacc cttggatatg tgttttgcct tatcctcttg ttaaatacca 198780cctatgagca aatatagtcc aaccatttac ttaacggaag gaaaattaat ttaccatgaa 198840gaaactgcac tgatgctgag tgcattgaga tctcttgtgg gcaatatttt tgagtcctta 198900ttgattagac ttcattggga cttaattagc tgtaatattg cataagcatt ttatcactgg 198960gaatgcagat cccacttaca taatgcttct gatggagaat atggcctaca gctactgcca 199020gaaagctttc acttagagga aggtaactct gccctatccg agtacccatc aagaaccaca 199080cagtgtctcc ctgggcgaaa gcactttgtg gagaggagca agagtgggaa tgggcgtcac 199140ttgcccgagc tataagtgca gaggcacgtc tttggaggtc tgctctccat ccagggagaa 199200aaacctgtgg tgctttggtg tctatccacc tgggaacaca tctcaaacca gagagctggg 199260tgtggtgcca cacctgtaat cccagcactt ggcaaggcag aggcaagaga atcacaagtc 199320accctcaact atatgtaagt ttcaagggca acctggggtc tatgagaccc attttaagac 199380aaaacaaagc tagacctcat ccgagtgaat caggcctcgc cctagtccca tgagtttatc 199440taagtttcta gaaagccagg gatgaaggtc cttgatagtg ttccagttat aactatacct 199500ttcaagcaaa ctgttttctg atttatggaa agcctttata gaagaataaa gaaaactcca 199560agtagaagaa gcttgattcc agaggttggg tgataactaa ctttgggttc ctgtgcacta 199620ggcacagttg atttaactac ttagttctca gcaggctcta actatcccgt gagatatgct 199680gaatgctggg ttgtttgatt ggtataaaaa tgaaggcata gcaaggataa gagatgctca 199740cttagaaaat gctagaacta acctttgaac cttagccctc tgaccccaga gagttcctaa 199800ccattctcag ttacagttag ttctcgtaaa tagatacctg gtaaggtttt cagagtattt 199860ggagagtcct ggagaggata tcaaagaaag cattaatagg aggccacgag atggcagtgc 199920aggtaaaggc acttgccaca aggcctgggg gcttaagttc acttaccaag actgcccctg 199980ccccatgccc acctacacta gagcttaaat acacattttt aaaacttctt aattaatagc 200040acatacttta gactctggga gatttctagc tccagccaac ctcttaccaa agctgtcttg 200100gttaaaaacc aaaacacaat gctattttaa gaatagattt aacttttcat tatctgtata 200160gagatgatga caaactttat tttctgttta tagagaaaag tggcagagcc aggtttgatg 200220gtgcatgtct acagccccat cagctggagg gatgagagca aactagtgcc agcctgggct 200280atatagtgag aagtagtttc aaagactggg aggggaagag actgagagac caagccagga 200340gggaatctga gaggatatga gaatgtttta tttgcggttt tctttctgga aaaagtagtc 200400agcattactg agacactgag tcacaaacat ctgaaggcct ggagcgcaaa gcaccagact 200460cctaaagcca tgtagctctc tgtgcagagg ctcctcacag cccctctcca tctgcgcagc 200520cagctctcct gttttgatcc ctgacctggt cagagcttgc gagtaacaga agctgacagc 200580agtgtgccag cttgcatcca attctgacat gcagtttaac tgtttgaagt tctgaaagtt 200640gttcattatc cacagaaagg gatgttttag agattggact gccggttaat ttttgttccc 200700attttggatt ttgagattta gaaaaataga gctgtctaaa atttggattg ctataaaagc 200760aatctataac taaggagtaa acattgatgg gaagcatgct aaaattgtcc aaaatataga 200820ctttggtgct gaacacttga gaatcccata tgtatttaaa catatgtatt gagcatatgc 200880cttgttgact ggaaagacta agagaaacta gaccaaacac cttcttgtac acacttagag 200940gaagcatggg tttctgggta attgtttctt acactcttac aatgccctct ggcaaacaga 201000tggcttctct ttccaccagc tcaaagaagc tgtgcgcgtg cagggacctg gcacgtcgtt 201060tctttgagac tgttcttctg cacagacccc ggagcagagc tttatgatgg cactacttga 201120aaggttcagg gatggcaggc tttccttcat ggggtcagcc cagagatgct ggcatcccag 201180tcccacagag ctcagttctc cttgtatgat tccttgattt gcaacgcctg tctcatccat 201240cccccacaat caccagagct caggcttcag taggactgtt cattttagcc cctgacctca 201300ttagcaggtg aacacgggta cacacacaca cactcctcac acatgcgcgc gcacacacac 201360acacacacac acacacacac acacacacac acacaccttt ccatgttctc ttgctcccag 201420tctcgcacac actcgctgtc agcccacctc acagtccttg tcatctcatt agtagttgtg 201480agcatgccct ctactgcttc gtttggaacc tgcttgtagg tgaatagtgc cgcaacagcc 201540tttggagctc acacagcacg tcatcagctg gatgcgagaa ttgtttggat ttgtttttct 201600aaagctatgc tgggtgctac ctcaaacact aaaaatggtc attgtttgtt tgtttgtttg 201660tttgtttgtt gaggcaaggc ttctgtgtag cncaaactgt cctggaactc actctgtaga 201720ccagactggc cttgacctca gagatctacc tgcctttgcc ttccgagtgc tgagattaaa 201780agggtgtgcc accacgtcca gcttctaata atgtattttt aaaggtagtg tttagattaa 201840gaattctcta ggggggctgg tgaggtagtt cagtggataa aggccctaac tacacaagcc 201900tgctaaccca agtttgtttc ctggaccact aaccagtcca tcctccctta aaggtggaag 201960agaatgaatt gaattcacaa agttagcctc tgacctctac acataggcca tgtatgcatg 202020catgtgtgtg tgcacacaca ataataataa aataaaccac ccccagaaat ccaaatgctt 202080agaagactga ggcaggagga ccatgagttt aggattaacc tgagtctggc ttatctcaaa 202140aaaacaaaga aaggacaaca ataaaaataa acagactcta ctgtttagta tctgcctaag 202200tttcttaatg tctcagtttt aatttcttaa aaaatgtaga ttataatagg tgtcctgtat 202260aggttgaggc tttacaggac gtagtactct gcctaccaca ccatagccaa ccatgataat 202320atgggcagga atgtctctgc aggaatcact ctactgacat ttcatttccg tctctctggt 202380tttgacccca tacagcagca cacctaaatc tttagtaaaa taatggtttt ctggcaactc 202440cagcttgaag ggtgggaatg actgttctgt tgtgacttct taggtatttc catggtagtc 202500agcctccaag tgaacaggca gaagccattg ttctccaggt ctgttgcttt tggtttggga 202560acaatattga gcacgcactt ggccttgatg acctcctgtc tgggccccta ggtactgagg 202620ctctaggaat acacctccac agctagcctc tgaggacggt gctacagctg tcagcaaggt 202680ttatcagtca ctgatgcgcc tgccatttgg gaggacctgg ctcatgtttg gacattgatg 202740ctgttctgaa aggtgcaatt gctgtcttgg aagcttccca ttcttcaaac atgagtctgt 202800gagtctgtga gtgacagcag gagactacca tgtgacgcat ttctagtgaa aatgagatta 202860ctacatcata cacagaccat tcctttatca ctgtcacaga gttttgagaa ttgggggtag 202920gtgaccaagg agttccttat gtccccttgt tatgcagaag agtgatctgt aaaataagta 202980gctgtgaccc tctgggaagg gtcagtgagg agccactttg ctggggcgtc ctgaagagcc 203040gacaggttcc ctctgctctg cttccttctc cgtctgctca cttgagtatc ctgtggttgt 203100aggtttgaag acggtcattg ccttcatttt cacatccttt ccatattttg tgtagaataa 203160cgtgtccaga gctcgcctga attcaatttt atgagctata tgatactgag aaaacgtggc 203220ttgatgctga gttcggccat gtttcaccct ctgcagcaga tgaaatgtgt tagcttctga 203280ccgtcggcca gtttctgctc catgtgacag ctaggatcag tgcttgccct tgtgccaccg 203340acacaaaaca gagtccctaa tctggggaag ctatgaaaac tctttggctc cttgaacaat 203400caaagattaa tacaagtttg tgtacataat ttcataattc cttgtgggtt ttttggtttt 203460tgggctttgg tttttttttt tgtagtgaga ctttcagggt ctcttaggat aaaatctgtt 203520aaggatgact gagaagaagt ttcagaagcc tggagttgag atttgcactg ggacttatga 203580caggttattt tgtttgtttg ttgttgttta aagaaattgc ctaggagaag aactctttgt 203640tttaaagcaa gcctgttaca gacatttttt ggccttggcc ccctaatgtc ttacatgaaa 203700ttttgtgcag aaagaccaaa ggaaaagagt ggactgccat ctagaggctt gaacaacaat 203760gttacaactt caaaagctta accggcctct gtgtactcaa ctttgtgaat taagaacaac 203820ttttttcttt cttcttctct tttcttttct tttcttttct tttcttttct tttcttttct 203880tttcttttct tttcttttct tttcttttct tttcttttcc tttccttttc cttttccttt 203940tctttccttc cttccttcct tccttccttc cttccttcct tccttccttc tttctgtctt 204000tctgtctttt tgtctttctt tctgtgatga cagacatgta tgtcatagat gcttcactgc 204060tgtgtctacc cttgctactg tagacataat tttagaatgg agatgccatg gtctttgaga 204120taggaactgt acttgaatat acctttactc acaaagtaaa aacaaatcaa tgtttttatt 204180tagcatctgt tttggatttt acattaaaaa ttaagagtaa ttcttttaaa tgactctgag 204240tgtatatttc atgataatga tgctactata agtggtgtag aaattgcaga gttgaatttg 204300ttctggaaaa aaatagatga ctagaatgta tatccgcctg atgatgcatt ataggacagt 204360caatagcata tccttatggt agctgaatgt tttaacattt caaagtcttg tttgttcatt 204420tataaatgtg catctttata gtacctgatc taggattgtt atgaagacta agtgaaataa 204480aaagcatctt cacgatacag tcttacttgg ttaagatgaa tattcaataa atattcatac 204540attcagttcc cattagatat aatgatcatt tttggtattg gatattccat attgcagtaa 204600aaaggacaca tgccttgcat tgtcgcagag attgtgtcag caagcacaaa tacgaacaag 204660agaagtcctt aaataattca tgtaaagaaa agtcacgtga cacagtgaag cgtaaccgag 204720cctgttttca tgtcagaggt cagagatgag tttctgagga agtgacagtt aaactctttt 204780tttttttttt tttttttttt tttttttttt ccatttttta ttaggtattt acctcattta 204840catttccaat gctataccaa aagtccccct tacccaccca cccccagaca gttaaactct 204900tacccgcatg acaggcagcc ctccatgaga aggcctgtgg agtttccatt tcaactagag 204960gactaagcat ggaggccaga cagctaagag aacttcactg tgggaaatca gacaggaact 205020ttttaggccc aggacttaac attttcattt tgagggtcat gagaagaaac tgtagagtta 205080ttaaagtggt gatgttagcc ttagccttag aaaaatgcca gtggagacta agacagacag 205140aggcaggaag cccgcttggg aaccttttct ggctgtctca gccagcagcg acggcttggg 205200atggaccctt tggctctgta ggattgtggt taacggcacc aggtcagggg ttaggggtaa 205260attcttgggg taaactgctt acattgctgt gtttggatcc ctagcaccca tgcaaaagta 205320gacatggtgg tacacacccg aaatcccaac actgagggtc aggatgcgaa gatctcgggt 205380tcaccagcca gctcatatag tggagcttca ggcttatgtg agtcactgtc tcaaaaagta 205440tatagtggaa acaattaagg aagccatcgt agcgttacct ctgacctcca tccttgttca 205500catagacaaa tgaccctgac acacatgcac acacccatcc ttgttcacat agacaaatgg 205560tcctgacaca cacgcacaca cccaggtaga catccagact attggtttgg atctgactgt 205620ggcctctggg ctgaacctgg gccatttcta aagtgggaag gatgttcaca aactgctctt 205680aggtttgtgg tggggctgag gtagcagttg taaagcgctg tgaatacgtg cgcaacgcgg 205740cctcatcact gtctgccgcc ttccctatga ctgttgctcc agcatggtca tcagagctcc 205800cagtttacac atgagagctt ttgtaaagtg ggtggctttg aaatcagaat ataggcttct 205860gttttggaac tctctctcca ggataacctg gagtgtgagt tgaaggcaga agagaaacag 205920aactaaaaca gggagccgag actctaccta ctgctcttac atctagccct atcagctcta 205980ccagcccccc agacccccca ctagactgag gacagctgtt tgtctgttgc agagtgtgag 206040cacttgatcc gccacatgct ggtgttagat ccaaataagc gcctctcaat ggaacagatc 206100tgcaggcaca agtggatgaa gctcggagat gcagacccca actttgacag ggtaagccgg 206160acccttgcct cagggaactc ctgattaact ccttctcttc ttcagtggtt gtgatctgcc 206220taggacctaa agagatgagc agtaggaacc agctgctggc taagaccagt ggcaggtgaa 206280ttcacaggga

gccgcgggat ccagcaaggc tgtggagaca gtgatactgg gcattaccac 206340agaaatcata gtttgctttt gacttctgag acagcatact cccagttctg caggtcactg 206400ccaccagagt gcctttcttg tcttgagtgc gctcccttca acagcttctg tcacatactg 206460ctgcacccca ttatgatcac tctcgtaccc caggagctca ggaagcagcc ctgagggttt 206520tgtcagccca tagctgattg ccagaactgt cttgcttaga cttcgtatga cacccacaga 206580atctgcagca gtggggcata gaacagcttt gtcaatgtgt atcccatgcc gtgttgatgt 206640agttaactgg ggtctgacgg cttgcctcta taactgtatt atattacagt taatagcgga 206700gtgccagcaa ctgaaggagg aaaggcagtc ggaccccctc aacgatgatg tcctcttggc 206760tatggaagac atggggctgg acaaggagcg cacacttcag gtacctaaga aggagtgcag 206820agcagtgaga taagcggccc ttcctcttgc ctgcctttcc tgcagtgtcc ccttcctgag 206880ctcgtgggtg ttcctgcttt cctccctttc cctcttcctt tgcccttcct ggacctgaga 206940gggaaggaag gagctgcttt gtgctgtgcc ggttgtgtct ggtgtgtcag tgaggcgcca 207000cctccgagtc tcactctccc tttctctatt ggtagtcatt aaggtcagat gcctatgacc 207060actatagtgc aatctacagc ctgctgtgcg atcgacacaa gaaacataaa actctgcgtc 207120ccggagcact tcccagcatg ccccaagcca tgactttcca ggcaccagtc aatctccagg 207180tgggcaatgt ctctgagacc taggtgagaa gaggttatac cagggcaagg gctagctact 207240gctcatcagc ttttggtcag gagagattca tattcatcca tctatctgta tctcctccct 207300tgtcagaaga ccatcaaagt ctgggtcttt ctttgagaga aagtcgcatg gcagaaacag 207360ttgtcttctt cgttttgttt aaagaagtag caaatcattg tgagatacca ttctccctaa 207420agaatgcttc cttgctgtgt tgcctgagag cagaagaacc aggctttggc aatcaagact 207480gctttgaata taatcctagc cattgggaat ctgaggcaga ggaatgagtt caaggctagc 207540ctgggttaca tagcaagagc atgtcttaga ggaagaggtg agggaagaaa ctactttgaa 207600aagctgtcat tatttcttct agtgtctcag gtctctgtgt gtctgtatgt gctgctttct 207660aggcggagca gacaggcact gctatgaacc ttagcgtccc tcaagttcag ctgatcaacc 207720cagagaacca aattatagag gcaagtagcc acatctgatc actgtcaact gaaaccacgt 207780ctcttcccat agctacaatt cctttccttc cttctgagtt acttctgatc aactgtgttt 207840ccctccacct tagcctgacg gggctgtgaa cttggacagt gatgagggtg aagagccttc 207900tccagaagcc ttggttcgct atttgtcaat gaggaggcac acggtgggag tggctgaccc 207960acggtaagta cctggtcagc atcctgctca gccctaagtc aaaaggttcc gccagggtga 208020gtgcctcttg tggcacgttt tctgagaggc cacactaggc catggccaat ctgactagtt 208080attctcccta tatgcagtgc ctcttagcag ccatgtgagg agagtgtaat aacatctttg 208140ttgatagatg gagttcttct agtctaagtc atcagaaata ttctgtagta ttcagtcacc 208200tttagaaaag tgtagtagtg caagcctttc atcccagcac ttaggaggca gaggcaggag 208260gagctcttga gttcaaggcc agcatgatct gtatattgag ttcaaggaca tatagagaaa 208320ctctgtctca aaaaataagt aaataaatct gccaacttta gcagctttta gagatgctat 208380ctacctcgtg ttctatattg gaactcttct agaaagagga gacaaggtgg tgtggcatca 208440tttatatatg tggacagtta gaggcacttg cctgacacaa gcaagaatca ggattcagta 208500tcctgctttg tctcaaaatg aacaggaatc ccaaaacata tttcccaaag tgttccttga 208560ggtcctcgtg ttggggaagt ggattcagga ccagctcact ccatttctac tctgcagtcg 208620agacctgtca gcatgagcga cagcccatct tgttggtgaa tgttttcctc actgagcagg 208680aaagagaggg ttatatatag ctagccttgt cctgagagtt cagctcaggg cggagctggc 208740tttcttatgt ctgtcttctg agtggatccc cagaagaaac gcccatgcct gctcgtttgc 208800ttgttttccg tgcttgttct cgatgtgatg aaacaagcaa gtgagagaac atttgggttc 208860cacacagaat gtgtcactgc caacgagggg tggagtcctg agtcgtgatt agtggaaaaa 208920tctcttattt aaagtctgct gggcatgaca gtcccgaaca tccagttctt atctgtcctg 208980tcagcatgga cggtagtaac cgaattcttt ccctggtgaa tcagttgcta cttcaggcag 209040tcattgtctt agcggatgcc ctgtgctctc tagaaacttg ctgttcccct ccaagctttg 209100agagcctggg ctagagttgt gccagcctgt gtgtctccta ttggctttcg aaagggatca 209160atgaagtaga ctccccaata actcagagtg aaattccaca taggagacag actccacttt 209220gctttagtgt cttagacaga aatagaaatt taggaggagt aacgtccgga aggtggtgct 209280tctgccggca tagagacctc agttcagatc ctcagtgttc atagagagcc agctgtggca 209340gcctctgttg taactacagc tctgtagagt ggagacaggc agatgcacca agctcacaga 209400cagcctgaca acaagcgaga ccgtgagaga cccgggagag catgggagag gaaggcttcc 209460tacctctggc ctccatactc atgcatagac atatgttggg gcactcacat gcatttacta 209520tcgtacacac atagactaca tctgcacaca catgtataca catatgaaaa aaatagaaaa 209580agtcagacag tggcacacac ctttaattcc agtactcaat agacagaggc aggtgggtct 209640ctgagttgga agccagtctg gtctatagag caagtaacat gacagccaag gaaggataca 209700aagggaaacc ctaacccaaa agaccctcct acccacctcc aaaaaagatt tgtgtgtctg 209760tatgtgtgtg tgtgtgtgta aaattttttt actgtactag ttgtagcggt agtattgact 209820actgtactag ttgtggtggt agtattgact actgttctag ttgtggtggt agtattgact 209880actgttctag ttgtggtggt agtattgact actgtactag ttgtggtagt attgactact 209940gtactagttg tggtagcatt gactcagact taagcattct gagtaaccac acttctctct 210000tcctgtttcc cgttagcacg gaagttatgg aagatctgca gaagctgctg cctggctttc 210060ctggagtaaa cccacagggt ccattcctac aagtggcccc taacatgaac ttcacgcaca 210120acctgttgcc catgcagagt ctgcagccaa ctgggcagct tgagtacaag gtaggaggcc 210180atggaaagga cctgcccaca agaaacaaac ttgatcttcc cttggtaggc tctgacagtt 210240ctgttccagt actggagagt ttcctgaggg aggggagttc ccttctgtaa actgctgtgg 210300tgatgggcga tatctccagc ccccctcacc ccagcagatc ctctcactgt agtagatgag 210360ggtgggctgt ttactccgtg ttttcccatc tcaccactgt atctgcctac tctcctggca 210420ggaacagtct ctgttacagc cgcctacact acagctactg aacggaatgg ggccccttgg 210480ccggagagcc tcagatggag gcgccaacat ccaactgcat gcccagcagc tgctcaagcg 210540cccacgggga ccgtccccac ttgtcaccat gacaccagtg agtagcagcc cagcctggct 210600ttctcaaagg tttttgataa gaagtctgta ataaaggggc tggacttggc tgaaataccc 210660acagtcctct ctaatacagc atcctgtgtt ttctctgacc tgcctcctct tccacagaga 210720cacctcggag gactttcccc agcagatgca gttcacacgg gcagtgctct ctagaatgaa 210780ctgcttttta attttcatct tataaagaca agctggcagg gggtgcacac gtgcttcagt 210840gcattcgtgg ggcttaaagg acagctttag ggggtcagtt ctccccttcc cccttcatgt 210900gggtagttcc tcaggcttgc aaagcaagct cttaccgctg agcacctcac cagctctcct 210960gtcgtcagca ccacctgaca tttctggctc agcctcgggt gatcgtgtga ttacattgtg 211020gctctaactt cagtcggtgt ctcagtcaca ccattttgta taatggtgat atgcaaaggt 211080ttaatctcac agattggttt ctgtttattt ctggtgtgtt tctgtcatgg tcctgtcaga 211140ctgaatagta tctccacagc ccttctgagc tatttggggg agtattgctt gaaatcacat 211200tacttggtga cttctgttca ccgatccatc gtggatcaat acgttttaaa acactcggtt 211260aacagaagct cactctggct ctctctgctt tacttctcct gggtttggtc acagagcggt 211320gtgtgagccc atgagtcacg ttgtcagttg cactgcagtt cagaaaacag ttgcaccaga 211380tctggtggtg tgctggttat gatatcaaag ccgagttccc ctgtatggag aaaagcttat 211440ggagccgtgt agtcccagcc cctacaagtc aggccaggag ggtcagtgca gccttgcagc 211500taacctggtc tacattgtaa gtcccagtct aaccacagct acaaggcaag acacagcctt 211560aaaaaaccag catgccagat tgaatgccaa agtgaatgac atggctgatg ggtggcagag 211620gctcttcatg ggcaaggttg gcattggttg gcaaggatca gcatcagcat tgtggatagt 211680ggggtgactt cctctgtcca ctggactcgt tgctgccaag gtcactaaca agctcttgtt 211740gataaatcca gtaggcattt ttaatcttta tctttttgga tggctcaact ctattagtca 211800ttgttgacct tcccctcctg ccccccttct ccgtagtact ttgtttttgt tttttgtttg 211860tttgtttttg tttttgtttt ttggcttctg tagttatcct aggttttcct tcggccagtc 211920agtcccctct gaggtttctg ctcaggattg acttagccac caaggcccgt ccccttcctt 211980ctctggatga gcctgtccac ttcccgtcag ctgccatctg gccttgcacc caggatgctc 212040ttctgagctc tacacatctg catgtgtgat cacctctacc ataggtttaa tagctacctc 212100acaagtgctc acagtgtgaa tgcagcatgt ggcacttgtc tttctttctt ttctctccca 212160gggataggga cagcactgtt tgttttggtt ttttatcttt atacaatttt tgttattccc 212220tctgtcagtc agttgtctga tgtttgtgaa taaacagatt tcatctctcc atctctgccc 212280ccttccgtca gccgtggtca ccagtcacct tgattaacac agtgactccc agcaggcacc 212340tggcttgact cctggttacc actggcttcc ccttgcaccc tgcaagtaaa gagaccccac 212400tttcccaaac acagatcctg taacaccatg cttctgctta aaatcctaca gggattcacc 212460actgcccatc ccaaaatcag tctgtgtgct cttcaaggcc gttgataaca gcgtcttccc 212520atgcctcagc catagctact tcttgtccaa ctgtttacta cagccttaga gctaaagtag 212580aggatgtgaa aaatttcaga agttggaatt atttcaataa ggttttgttt gtttttgttt 212640tgtattagcc agttttaaag ataatattta tttttatttt tggttaatct ttttttttct 212700tcagtttttc gagacagagt ttctctgtat agctgtcctg gaactcactc tgtagaccag 212760gctggtcttg aactcagaaa tctgcctgcc tctgcctccc gggtgctggg attaaaggcg 212820tgcgccacca cacccggctg tttattttta tttttaatta atgagagaag tgtaccagag 212880ggcaggtgct ggcagaggtc aaaggtgcgg ggtcctttgg agtttggttt acaagttgtt 212940gtgaaccacc cagcatgagc aaggggtcaa actctggacc tctgcaaggt cagtgtgtgc 213000tcttagccct gagccatctc tccagccctg gaatccagtg ctttagacct gttggccatc 213060aagtatggtt tctaaatagc acctgaggca tgggtgatgc ttatctgggc cactgggcca 213120agaatatttt ctgagggtgc acaacatttc tatcttcacc cagggaagag ccaggaacct 213180cagtgtggta cttctcattg cgtttatgtc tcagagtata gtgagtattt aaaaacatgg 213240aattacaact atttgattat atgttatgca ccaatcacca tgatgctttc ccggagttca 213300gacatccaga ctctggagct gacattaatg tgaggccacg gtcagttcct aggcactcta 213360ggtaggcagt tcgatgcatt ccaggtcctt ggacactgta ggccttccct cttctctcag 213420cacatgaccc tttgctctct gctggatgtt cactcctgtg cacacctcgg gtcacaggcc 213480agctgctgct tcctccaaag tgccttcttt ccttcccaag ctcccatgga ggacctgaga 213540gctcagtcgt gcacttacga ttgtctgctc gtttttctct aaacaaaggc aaagaccaca 213600gctatgaatg cgcagctggt cctaaaggag gcctcgagta ttagatgaac aagcacatgc 213660ctgtgaacaa gggaagaaat ccagatatag cacctgagcc cgatggagct ggaaagcaag 213720atggaagaaa gtcctctcta gccaccgttc tagggaagcc tttgaaataa gaacattaac 213780aagacccttg gcctcagtcc agttaccaga gttgagggca tcacagaata agctgtcacc 213840ctgagccttt ccttcctgtc tctctgcagg cagtgccagc agttacccct gtggatgagg 213900agagctcgga tggggagcca gatcaggaag ctgtgcagag gtaatgtggc acctggcagt 213960gcttgcagaa cgtccttggg caggctcccc tgcaaccagc gaaaggtcac ctctgcccag 214020ggaggtggtg cagtgcagct tcaacacttg tgtggtctct ttgctcaagc cagatggcat 214080gttcctcccc acacggaagt aacagaagac tgcccagaaa atttcatttc tataagcgcc 214140attggaagcc ccttaagtga cagcaatacc cttaagaacg ctttctctcc tccctccctc 214200ttccttgtcc ccgcgttcat attgcaactt tggtagtgtt caggcctaac gcctgctcct 214260ccctgccacg aactgtttac tgaaaaggag ttggagattg gagatgacag tgatcctagc 214320tagtctggcc atggacctct ggcatggact catccgtccc aggagaatga atgaatgaat 214380atctcctgca ctgtttgctg tgtgtttcct ccactccctt gtaccaacac tgagatattg 214440ggaagaaaaa acaaacatgg gggttggggg gaggggcagg gacagttgta aatatccatt 214500gtttgcagaa ccttgttgag agatgcctga ttcacagagc gacagtgacc atcaggtgtg 214560ctgtgcagtc cgatggcctc agttccagta gccatggtct gcagctccat gcatgccccc 214620ttgtggagtt gtctttctta ttttctgtga aggggttttc accagaatcc agcatgtatt 214680ttcccagaaa tacaggtgtt cttcaagatt atatgttttc ccttgaggga agttgaaata 214740aaatggtttt catgggctct cggggaaact gtgggtgttt ttatcaatgg acattaacca 214800cagatggctt ctttaagcaa gtttgcatac tacaaattac agtctgatga cagcttttaa 214860ctgacgtagg caatgtagtc aggaatgaac taagagcctt atggttgttg ttcaggaatt 214920gttcttcacc cagaaggtct ataggaaaga ccaaagaagg tgcccttgtg cagaaagaaa 214980gccctagtga tcaggggtaa tgctggccta gtcaacataa ggaaaacaaa aaacacaaaa 215040acaaaaacct ggtgactttg cctcagtctg atactcttgc ttctatggtt tgctgataaa 215100aatgaatttg caaatgcgtc ctactggtgt cttgaagaaa cgagaaagta gggaatgttc 215160tggatccccc tccacccctt ggaaaggtgc agctgggttt taccttcttg ccttagagtt 215220cacgtcctct gcccccacct tcttcagacc aacattggaa ggccagtgag cctgtcagtt 215280ggaagggaca gaaaggagtc ctgcttctct tatctgtatt caagacaagt gaagtgctgc 215340agggggcctg gagcaaacgc aggcccccgg gtcttgttat ggtgctgacc tgtgtaggtt 215400cgcaagctcc gtagagtgaa tggcttcatg gagaccttgg cctcctcttc tgtttccttt 215460cctttcttct ttacctcctg aaaatagatc tgaacatcct gtgtggtttt atttcttcat 215520cctctaagtc tgggagccac atcagttttt cttctccagt gattgagttt tggtcacagg 215580acataaggga agaactcagt tgtcctcaaa caagagtctg ttttctttgg catgtagtaa 215640ggacaccaca ggcctctgac ttcaggggac gtaaattgat tctttctgaa tacctcactt 215700gtccctttaa taggccttag gttctatttc ttctaactta tagtttagaa atttggaaaa 215760aaattaatat gtaggagaga taacttcttc tattgaatgg aataaaggaa attggttcca 215820ccgcctttgc aagctagatc tccctttctt ctcttaatgg cgactcgcaa gctcgaaagc 215880agccacctca gtggtgagcc aactgttggc cggttaggca agcttcacct ccatcctgtt 215940gggggttgct tactgtggag cccttttggc accccatact gtcctaccca gtgcttcaga 216000ctaacttctg ttgcttcttg cgcgggaaca ttcttgtctt tttttactat gaaatgtcct 216060ccctgcttgt tgcaggtact tggcaaatag gtccaaaagg cacacactgg ccatgaccag 216120cccgacagct gagatcccac cggacctgca acggcagcta ggacaacagt ctttccgttc 216180ccgggtctgg cctcctcacc tggtacctga ccagcatcgg tgagtagccg cttggcagtg 216240tgagcatccc ggaggctcat tggatcagca cccttctgac tagcctccag cttagagtga 216300ctagctctgc ctagcagcca cctgccatcc cgagctcaca cctccgctcc tagccctaac 216360caatgcacag ggcaaaagct ttctcttgga ttctggagca gccaaggctc ttccatctca 216420tttcttagac cacctactca tcacctgcat ctgctgcatg taggcctttg agagagtggt 216480actctcagaa ggcagctcta gaagtaagga ctttgtagca acatccccaa acatgaccgc 216540caatagttca tcgccttcag aaaatgggca cttgatgtgg cattagagca gcaggctggc 216600tgtagacaga ggcataccct tgttgtgtgg tagaacagga gtgttgcatc cttttgagtg 216660ctgtgtttgg gaagtaattc tttgagtatt ctgacagcaa gatcttttgc taggcctctg 216720ttatttagaa ttcattacct tgaagagaga aaccaagttt ccctctaact ctaactagat 216780aaatttgctg attccaaggt agcctgccag caagagagct gattgtcctc catgactcct 216840tgatgactgg cagtcttagc catgtccatt ctgatgctgt cccctagctc ccaggtcatg 216900tttcccaagt cgtcagccat tgcttgatct tgtttcttcc agtcttcttg gggtagggtg 216960ggatagtgga atgcagcctc ctccagaagg cgaaagcaga tgggttctga ggaaattcag 217020tacacatgtg tgccaatcgg agggaacact tgcaactagc cactcagatc agctccatac 217080agggttctca ttgtgcttct tgtgagcagc aggtaatcag ctgaggcagg tgaactctga 217140gcagacaggg acataattag tgccacgctg gaactctgga agaaggaaga taagctttca 217200taattgaccg tgaacaattt agaaagaaaa agtcctatta ggaactaaaa gaaacggtga 217260aggttctcat gcaagattgc gatggtgcag cccccgtgac ggaaacccct gggttgctgc 217320agtcagtcac ctcagagagc gcctctgtgc tggctggctg gcgagctgcc cccaccccaa 217380ggcagctcgc atcgtgcact ggtcctgaag gtgctttcgt tgcttctcac ctcttcctcg 217440tctgatattt gacttgtcag ccctccccca gggccttttg tttacctttt gtccattgaa 217500tacctggctg tacttctccg tgctccttgg gaaaatggtg atgagcactg tccagccagc 217560cttcctcaca gacgctctat tctaccaaca tttgttaaaa gaagaccaca gcagcctgcg 217620gacagggtcc atccgtggga catgcattgc gcctgcagat attgccaggg ccttgctgcc 217680tagagccctg ctgcctgttc ccatctgcac atgtgttacc tgggtttcta gggccaaggc 217740agtttgagtt ggaaacaaat gaatgcttga gtgagaaatc atgtactgtg gggcggtggg 217800gtcactatac aacgcacaag agattaatgc taattcagaa gaaaagagaa gtgttctaag 217860gccgtaaaac acacgatgtg acgcaggcag cctgtctgcg tcaccggatt cctttcgttt 217920tctggagcat gattaaaaga acagaatata tatctattct ttgtgttata ataggtgaag 217980tccaccatgg agaccgaaga ggggctggtc aacatttaca cccctggaag tgtatgcttt 218040aaaagttggc acaaaacagg ttctatccag ttgggggcta catcactgtt ggaagagtat 218100aggggagata gcagcctatg caaggctgcc ctgggtgacc gtggttgctg ttgaaaacaa 218160agttgtacac gcttgacact gcccatggct ctgcagtggt caggtccctc ggaaccaatc 218220ccctgaagtg actgagccag gctgagctgg ggccgacttc acagcctgag agtccggaaa 218280acggtgccac accttgaacc tgggatattc ttgctataat gagaaagaaa tggtttcatg 218340cagaaagaca gctcgaagaa catccctggg tgacggaaac ctgtctattg cctgaaaata 218400ctgaaagtgt aggcgtccga tgtgagggga gataataccg gtgctgggtt tccattcaga 218460ggctgaagtg cagagagaac aggtgtctgc aagcatcctt ggtgaactca gtgtctggac 218520cgtgacatcc tttccccagc cctgattaat ctgccttgta tgttactaaa acaagtagga 218580ctccatgggc atttcactag ctcaccactg cttgaagctt tgatgacgtc tgagtaaggg 218640ctttcacccc gacatcagca ctctcttggc ctcgctgcct ggtcctcatt tctcctcagc 218700ccttccctgt ctgcactgta tttgaacttg cttgttcgcc tgcttccttg gttttggctc 218760tgcttcagtc caaactggca aggttcccca ccccctcacc attggctcag cgtgatgtca 218820tctctgcctt agacctgcta ccctgaccct ttccatcagc tgtggggacg ctcgctgcct 218880ctgcagtctt taacctgctt atctcagctc ataaacatct ctttctggct aggcagcaag 218940tgtaggtgtt agccagcccg gtgctatctt agggctcctg atcaacggtt cttctttctc 219000cccataagac atggctgtgt tctgactgaa cactggggac ccctctgtca gcaggaagtt 219060gggcaggtgc tgctgtgtga ggagatagca ttcacagtag gaaggaggcc tcatgtgtgt 219120tcataaggta cgtgcaggat cttgaggccc acccacattc ctgaggaata cgctgtctcc 219180gtgctgggat cagaggtccc tgtcttcccc caccccagcc tccaccctcc cttctctaga 219240acagaactgt gagttccagg ccagaccttt gaccacagaa ctgactttgg tttcatccta 219300gccacacata gattactgct agaggtaact cagggtcaaa cggtgaattc taagtttcta 219360aagataccag gcagggatct ggttttaaaa accatagctt gcacaacatt atccacacac 219420cgccccccaa atgcaactac aatagaaaat aaatgagtga ctataagaac caagcctgtg 219480tggttcagcc agtcctggcc gtactgcgga gttgcaccct gctctagtat gtttatataa 219540gaaccggatg tagttgaccc caggacctca gcaggcctgg gaagtaggac caacctgagg 219600taagtggagg tgggagatga gggcctgtag tccactcagg gggacagcca cctcaggaag 219660tgacacggag taccatgtgc agggctcctc atgcatccac tgtctctgta ttcatgtaga 219720cccatggtcc cgtgtgattg gtcataaccc cccgagtcct ggctgctaac cgtctcttcc 219780catatattca gctccaccta caaggactcc aacaccctgc atctccctac ggagcgtttc 219840tcccccgtgc gccggttctc agacggggct gcaagcatcc aggccttcaa agctcacctg 219900gaaaaaatgg gcaacagcag cagcatcaag cagttgcagc aggtaatctc tggaggggca 219960gggggagcag tacagtgcca ggacccgggc agtaactctc catacccttc atgtcaacca 220020cactgaggtg ttctagctta aagcagaata gctttcttca atatctactg ccctccctag 220080ggtcaaacct gcttccctca taatgagact aaaaattttt tcccaccatc tggaattcat 220140tgggaaggca tttctggttt gatttttttc ttgggaacgg tacttgtaag caatcttggc 220200aggccccttg tatgcagggc acggggtcgt agtggacccc attgctgtac tttctcaccc 220260ccggcataat ccccagctgg tattgctccc agaggtagca aacctgctcc caggatactg 220320tgacgagcca agggcttcaa gtagcacgtg ctctctctgg gtttatagtt gattccacac 220380tgggcattcc acagtgtgtc cttggcgttt cccactgctc cccagagagc agggcgtgag 220440gatcactgtg tgcatgccca acaaggatac cgttcttcca tggctcattc cgcccatgag 220500accttggggg cagatctgcc tctcctgagg tcatggcatg gggtagactt ggccgtgtcc 220560cgtttgtccc cactgctttc ccttcaggag tgtgagcagc tgcagaagat gtacgggggg 220620caggtggatg agaggaccct ggagaagacc cagcagcagc atatgttgta ccagcaggag 220680cagcaccatc agatcctcca gcagcaaatc caagtaggcc ctcactccct cttcgctctc 220740tcagaatgcg tttttgagtt tgctggcagg cacattggcc tgtgcattgt ctgaaagact 220800cagggcagcg tggagtgtga gccacgtttg tgggtgacat gtctgagtca cagcgctttc 220860tgccccttcc caggaatagc cctaatgcct ttcgtagctc acgatgctgc cgttcccccc 220920ttttgtgttt taggattcta tttgtcctcc tcagccctcc ccacctcttc aggttgcctg 220980tgaaaaccaa ccagccctcc tcacccacca gctccagagg taatgaacta cttagcctct 221040tcctgcattg ttggctggct ggacggtgtg gctcccttgt ctgacgcatg gggacgcttg 221100actgacttga gcatttggga tagtcttcta gaagaacagg gaagccgagt tagagtttga 221160agggcttact agaatgctga ctatttcctg agctccatga acacagtggc ctgacatcca 221220tcgaaggcca ggccagtgag tgctgagttg gtggcaggga aggactggta gactgaggtg 221280tgggcaagct gtggtggcct gctaccgcag ttgtccgccc caaactacaa actgtctgtc 221340tgcatggatt

gtctggctga atgttaactg cagatggttc tgtcagctca ggaggcttat 221400gtacctgttc tgtccttttc taaggttaag gattcaacct tcaagcccac cccccaacca 221460tcccagcaac catctcttca gacagccaag taacagtcct cccccggtca gcagtgccat 221520gatcacgtct cacggtgagt agaggccgag ggagtggata catattgtgc cgtgcaggga 221580aagcctggct tttaggcttg gatttgttgc tggtggtggt ggtggtggtg gtggtggtgg 221640tggtggtggt ggtggtgtgg tggggtgtgt gttaaacttt gtttcttgac tgtgaaacct 221700tgggtaataa gtacttggct ttctcaacaa taactgtaat aatagcaaca cttaagaaat 221760atatataaat cacctgggac agtacccaat ataatagtcc ttaatacagg ttagccacta 221820ttgatgtagc tattaaaacc tctccagtcc tcgtactagg tgtgaagact atgggtgcag 221880atcctcagac ccagcacaca gtcaggtcct tgccagtctc cttggcattg tggagaaaca 221940tagaattgct cttcctcctc caggtgacct tgagtcagag ttggctcttg ggttctgtcc 222000cagaaccacc gcacgagaat gtgcagctta accaactctc aggatcattc ctgcatatcc 222060ttggttaaga aacactgcat acaaactccg ctaccctaaa tccagccata gagcagctct 222120tgggggactt aattggaaat aaaattgcag gggagcccac ctgaccgact gagtcctagt 222180tctgtgggta ggacccagtg agttagatct gtttctgcca gtttctggtc ttgtcttgta 222240tagtgaggtt gaagaaggct gttctagatg ctacctgctg tggggtctcc tctagctggc 222300aggtaacctg ggcacttggt caccctgtgg tccctttctc tgcaggtgct acgtctccct 222360cccagtttca aggcttaccc tcccatggtg caatcttcca gcagcaaccc gagaactgtt 222420ccccgcctcc cagtgtagca ctaacctgcc tgggcctaca gcaggctagc cagtcacagc 222480cagtgactat ccagctacag gagccagttg acatgctcag caacatggcc ggaaccgcgg 222540caggctctgc agggcggagc atccccatca gccccagcgc cagtcagatt cagatacagc 222600accgagccag cctaatggct cccttcagct atgggcaccg gcccttgtcc aagcagctga 222660gcgctgacag tgcagaggcc cacaggtgag tgagaaggga caagtctgct tgtgctctca 222720tgtcacccat ggtcaaagcg gcatggtggg cttgttgatg agacaggttg ttagtgagag 222780aagaggaaat ggcagagggc cttcctgatg gcctgaagga aggaagatct gttagaggaa 222840catgtctgtt cccctgactt attattctag ggttcttcct tctctgttgt ctctaagaga 222900ctattattct aaacagctca gattattatt atttttgttg ttgttgtttt gttttgtttc 222960gttttgtttt gagacagggt ttctctgtgt agccctagct gtcctggaac tcactctata 223020gtagaccagg ctggcctcaa actcagaaat ccacctacca ctgcctggct cagattattt 223080tttaagaaca gctagattag actgtgtgta tagctctggg ggtaggaggg caagcctgaa 223140tccctggttt cagtccctgc caccagagaa gctaggtgtg gtagcacatg cctagaaccc 223200cagcgttcta gaggcagagg caggagtggc aggagttaga ggtcctcttc agcaacatag 223260cgagtacggc tagcctgggc tgcatggaga ccctattcaa gaaactatcc aaggtcagaa 223320tgttgctttc aggataatta catgcccagt gccttatggc tcttcaaaaa cttggatgag 223380cagataaggg gatgaatagt ggtggtgtgc acagtcctcc ctgccacagc acaggtctgt 223440accatgagag cttcgtgcta aggaaggcga gtgactgtcc ccagggaagc tggtgtagtt 223500actggaatgc ctggtactca taccattttg tgtctgcagc ttgaacatga atcggttctc 223560ccctgccaac tacgaccagg cgcatttaca cccccatctg ttttcggacc agtcccgagg 223620ttcccccagc agctacagcc cttcaacagg agtggggttt cctccaactc aagccctgaa 223680agttcctccg cttgaccagt tccccacttt ccctcccagt gcccagcagc agccacccca 223740ctataccacg tcagcactac agcaggccct gctatctcct acaccgccag actatccccg 223800acaccagcag gttccccata tccttcaagg actgctctct ccccggcatt cactcaccgg 223860ccactcggac attcggctgc ctccggcaga gtttgcacag ctcatcaaaa ggcagcaaca 223920gcatcgacag cagcagcagc agcagcagca gcaacaagaa taccatgaat tgttcaggca 223980catgaaccaa ggggatgctg ttagcctagc tcccagcctc gggggacaga atatgacaga 224040gcaacaggct ttatcttatc aaaatgctga ctcgtaccac cgccaccaca ccagccccca 224100gcatatctta cagatcaggg cgcaagattg tatctcacag ggtccctcgc ccacccccac 224160ccatggctat gcccatcagc caccactaat gcattcggag agtatggagg aagactgctt 224220gtgtgagggg ctcaaggagg gcttcccaga caagagctca agcacactga ccaaaggttg 224280ccacaacagc cctctgctct tgtgtaccag tgggcctggg gaccctgagc ctttgctggg 224340aactgtgagt caggcccggg agctggggat ccatccctac ggacaccagc caactgccac 224400cacgttcagt agaaataagg tgcccagccg aggtaagagt cccctcagag aaagactctt 224460gggagagttt gctggaattt aagtgtgcag gggtgttagc ttggttctgc tagtattctg 224520tgcgcctaag caaaacaaaa caaaacaacc ctaaacagac cctaggacca tttgggggga 224580gaggtgaagc atgtggaaag gcgattggtg ggtgagaagt cacctacaaa atagacagca 224640gtcgacaggt ggcacccagg acactcctgg tcacaccacg gggtcctaga gtccagattt 224700gcccggctgt catcagaaag tcgcagtgga aaccgtcagc agtactaagg aaatgtgtaa 224760actgtggggc tgagagtcag agcctccagc ccgtcctcct ctgtcctgac tgcgagatct 224820agactctatt ctcatcgtcc tcttgtgtga tagaagggca atggacactg gccctctgca 224880gcatcttgtg acgatccgag catttgtgtt cgtacagact gcctctgtgt gtgtgtgtgt 224940gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gagagagaga gagagacaga 225000gacagagaca gagacagaga cagagacaac ttgataacta agtatgctgt atactgtact 225060ggggctgcct ccattttcat ctaaagatta gagtttacct gagtgagttt tgtttgcata 225120gcaaaaccag agcatcagac cccctgtagc cttttgcttc accctcctca ccacaaccac 225180agagggagta aagcgactta cccacagagt ctgggactct gggcgtttct tcctcattcc 225240ccctggagtt acccatatct cttgccttta aaggcgatgc acacaactcg gacccatggc 225300tggccaagcc ctgcccagca gcaggtgtgt gaagacttgg tgctgtcaga tggacttggc 225360atgtgctcag agaggaggga aatgtgctgt ataaaatgac agtttaagtc ctgaccactg 225420cagccaatgg tgtcaccagc ccggagatct gccctccaca tgcatgttga actctgctgg 225480ttgtatttag agtggaactt tataaacagg ttgtgtgtag aatgagagac acctagcagc 225540attgggggtg gggatggaac aactttgaag gagcagatct gtcagatgga aggactcaac 225600ttttatgtgt atgataagga agagactcta aagtgggtag aaatcacggt agattatagc 225660tctccagaga agactgttac gagagactgc catcacaggc tacctgagca gctttttgtt 225720gggtgtgttc tggaagaggc ggatgccttt gatggacaca ctgtagaaga gcatccatgt 225780gtggaaagtt tctgatgact cagctcgctg aaactgcatc gtgcatgcgt gcctccagtg 225840tgcacacacc gagtgctgct ccatcctttc ttcttattgt gatgctaggg caaaacccag 225900ggcctcacac acatgctagg tgagcaagct ctacacggag ctgcacccag cctgttgtcc 225960gttttaccag tgacgcagct aagacttagg agatttataa tttgcctctg ggtcagagct 226020gacaatgact gccaaaccct gctgccagtc gatcaccacc ccaacaatcc atgaatgtgt 226080ctgatgttca ggctactgaa gaaatagtgg agcgggtccc agaacagtga agccatgcac 226140tggaggcaga gatgcattag tgaagcccag tgcatgctgc tgtctttaat tcagatctcc 226200ttggggccag aggtctgtgt gttaaaagtg attctttgtg gaggtgcccc actgtcctta 226260ggctagcaca ggttgccttg gagcctacta ggtaagcaca cttccgttta ttctggcagt 226320gaggggtgcc aagctaaata aactcctact ctatgcttgg cgctctgact caagacatcg 226380gttccttttc acacccaccc tcggaagtgt gtgtcaaggt accattttgg taggagctgc 226440cctggcctga cctgacatgc ctgtgaagcc ttcagttgtc cttttcctct ctccccctcc 226500ctctcccctc tttttaagga cagggtctca ctgcataacc caggctggcc ttaaactcat 226560gatctccaag tcagctgtgc acgtgccggt cggggttcca ggctatgcac cacaccaatg 226620ttttcagttc tgggaagaaa ttccagaatg ggctggggag agggctccgt gggtgaggct 226680ctggccacac aagcctgaca gcttgtaatg gtggaggaag aaaagcagct cccaattcac 226740ctgacctcca ccctcacatg catcacacac acactcgata atgagataga aacttcacag 226800gtcatttgcc atgggttttg tgaagaaatg gttctaacag tgtgctgcac tgcaggcgac 226860cgcagaaccc gcacccatgt ctccctgttt cgacagagga ccttgatggt tcttggtgcc 226920cttccatcaa acaggctagt ctcttggcgt ttaacctatc actccatggg agggcagctg 226980agtacaagtt agtgagccag ggtcatgggt gctgcgggaa tgtggaaagc caaagggcct 227040gtcttcagga agaagagctg tatcagaagt ggcgtggaat atgtgctaca gttcacaaga 227100gcagaactct gcatttattg gcatttctga agggtgaatg ggtaaggctt accaatgtta 227160gtctaggaaa cttagatgcc ccgttacccg accgagtctg tgctttcctg ttccctataa 227220agctgtttgc caccagccag taggaagcca gactgggcag agcactgagc acctactgtg 227280ccagaaatgt cctccagacg ctgcaagtgt cagagctaac ctgccttcta ttgcctacgg 227340tctcattgtc ttccacttaa ggagtgaaga taaggtgggt tgccctcctg cagtattgca 227400ggagcttaag gcttttgact ctgcacgtaa atgaatacaa ctgcacagtc agttgagatt 227460accaccttaa actcagatag aaaaccgcat cctcttcttt gagcccagtc agcttccagc 227520cttgtaaggg ataaagtgct gacagccttg ctggggttaa gacagtagcc cttagagcct 227580ctaagagggg tgtgcaaaca tgccgtttct catttactct taaaacccag taaatgaata 227640aggatgagtc aacattgaca gattgcacag ttacgcaagc tggcccagag tcccccaggg 227700tgtgggggtg tgccttggat tctcattgtg cattttgtcc cttacttgtt tgggtactct 227760cccctctgta ccctctgggt tcttggaaga catagtccct gtattattta tagatccttc 227820tgtaagcctg gctgttctct tactcctaaa atggcctaaa gggtgccaaa ggttctagtt 227880ccctggtcag tgggttagtc tttacagcca gccactggtc agcagggctc agctaaggac 227940tttgagtccc agactcagca cccttccttg tctgactcgt ttacctacgg agccctcagt 228000gcttatcgca gaccttccac tggtgttagg cttttggagc cctggaacag ccctttcctg 228060tcagataagg gacattaagg gtaaggggag actgcctggc cgtcaggctt tgtgtactct 228120ggtgataacc tggaacctgc ctcctgcgac cctcattctt ggcccctgta tgtattcttt 228180ccccatgtta gcttcctgtc actgggacaa aatacccaag aaaatcaact tatgaggagg 228240gaaggtttgt cctggctcat ggtgtcagca gtctcagctc ataatttgtc cttgttgctt 228300tggagcctgt ggagagagac atgccatggc aaggggcatg ttggacaaag ctgcacttta 228360ttctgactgg gaaacagaaa ggaagccatt gaggacccag ctcccaaatc ccagtccagg 228420gcatatccca gtgtcctaac ttattttctt ctaaaatttt tctcatttat atttattttg 228480tgtagtgggg ataagtgggt tgtgttctgt tctctccttc tactctgttg gtcccaggga 228540tgaaactcag gcttagggta agcaccttta ctcgctgagc catcctgtca ctcccaaaag 228600ggagaaggag ggagggagtg tgtgtgtgtg tgtgtgtgtg tgtgcgcgcg cgcgctcagg 228660acagttgagg ggagtcagtt ctcttctccc acctatgggc cctagggatt gaactcaggt 228720catcagactt gatggcagat gcgtttatgg ctgaatcatc tcagcggccc caccctaact 228780tccctttatc aagctttgcc tcctaaaagt tccattatat cttcttagcg ccacatactg 228840atgaccaagt ctcaacatgg gagcctttag gcaacagcca agatcctcac agggccctct 228900ctaacccttc ccaggttcct ctctagccct tcccagcaca gcttctgagt gggagacact 228960tcattttcct ctactgcggg gggtcttgtg atgcttaagg ctcaaacatg gtcctacact 229020ctctaggcaa acattccact gcagagctgt gacctcagcc ctggatctgg tttataatct 229080catttgcttc tttcagacca aaggctgcta ccctggtaac taaaaattgg gtaacttact 229140tgtttattgt cctgctctgt ctatcagcag cctgtggggt aggctccaga ggcacaggtc 229200cagaaagatc cgccacctcg ctcaagttcc cttccacctc ctcaggaagc aatgaagcta 229260gaatcaagct gagtcttccc accccggcgg tgcctgtggc acctttgcca cctccacctg 229320acagagaagt gagccctcag ctgggcactc aggagagctg gtcaagtgat agcctcttgt 229380gacctgaggc ctgggaacat cactcaggaa agcagacagg agggcacaga agggaagtgg 229440agcccacaag acctttgaaa agcctgggtg ggagcttaga tttggagaaa cctcattttc 229500aggtctaaaa aaaaaaatcc aaaaggggag aaagagaatt taacagacag gaaaacacat 229560ggggggagag tgctggtctg gaagagggag tggggtggaa atatcaagat tctgttagtg 229620aagagaggcg cttgccatct gctcctgatt gatcacaggc cttaaagtct cggcccaagg 229680tcagaggtgg cagagagtaa acccccatga cctgagtttg agccttagaa agggaagaga 229740aagccaactc ctgaaagttg tccctcacct ccacatacat gccataactc cacatacatg 229800ccatagcgcg tgtgtaccca tgtacacagc taacaattgt ttaaatgttt tcaatctcta 229860aacaagggat ggaaacagag cactacatat atacaacaca tgcaccacat ataccacatg 229920tgtacacatg tactcatttg catgtactgt gacatgcaca catatgcata tattttaaaa 229980gtaggagctg ggcgtggtag tgcacacctt taatcccagc actcgggagg cagaggcagg 230040tggatttctg agttcgaggc cagcctggtc tacaaagtga gctccaggac agccagggct 230100atacagagaa accctgtctc gaaaaaacca aaaaaaaaaa aaaagtagga acgggatttt 230160ttttaaagtg tggcctttat agggaagtaa ctaggggtgg ttagaaaatg atctaggcaa 230220gagatactgt cccttgactg gaagcaggca gtctgggggc tgaggtgtcc accactgaac 230280gagcggactc tttagactta gccgctgtgg ggccgggaag aaggaattaa gcatgactcc 230340aggaacttac acctcaaaga ttgggggaag ggaggcggtg ccaaacaagg gctcttaaag 230400gagtagcagg cttctgggga agttaatgag tttgaaattt aacgtaagtg ttacatacta 230460gtaagataac caagaaaaaa agttggatct atggatctgg aaccaaaaaa aaaaaaaaag 230520gatttcagta actccgttta catttagtgt gtgtgtgcgt gtgcatgtgc gtgtgtgtgt 230580gtgtgtgtgt gtgtacatgt gtgtgcacat atgtatgagt atgtgtgtgt gtgtgatcat 230640ttctcttact aggcgggttc tgaggataca actcaggtca tcaagcctga tgacaagtgc 230700ctctcttcac tgaacatctg gctcattcca tccactgcag tacatgagca gtgtggctga 230760agccatggac ctagatgatg tcaaaatggg catggggagt gaggtcaggc acagctgtgt 230820gcaagcactt atgaaaggtg aaggagccat aggcagcacc agcagtggcc gtgggacagt 230880gggagagacc tgggatggca ctaaggcaga ggccaaggcc atggatacag gccagaagcc 230940cctcatctct agagctctcc atgagctcaa tcaggagctg agcacaagct aagccagcag 231000agtgatgggg aaagaagaag atgggtatgg cccagctcac accccatagg tggatttttc 231060cttctagggt aaggagtgtc ttaaacccct atttgctgta ggatttgatg ttgttaggct 231120ggccccagct cctctaacac ccatgccagc tgactgagca ttggcatttg ggcaaatgtg 231180gtccctcccc tctcactcat tgtgtcttac aaggggctac atgggagttg gcgtcacctg 231240tcacctggag aagctctggg cctgctcacc ctgctactct tgctctgtct cctagagtct 231300gtcctaggga actgcctgga aagaagttct cctggacaag caatggagct gccggatcac 231360aacggccttg ggtacccagt acggccctta gtcagtgagc acctcaggtc ccggacgctc 231420cagagacacc acacgatcca gaacagcgac gatgcctatg tatgttggcc cctcctgctg 231480cttagaggtg ggatggtgcc atctcaacct gaaagcagag tgtccccttc tgccgccatc 231540tcctcagtcc agccctctgc aagctgtgga gacctttttt ttttctttga ctccccataa 231600cagaccagca ttaacaggag agtcttgcgg ccatatgtta gacttaggaa tcaaatgatc 231660tgacatccag ggtcttgacc tctcccagaa atctaaatcc aggcttcaat atatgaaccc 231720tcaatttttg gtccattcct ggcaactgtt ttgctttctt tggctttctt aagagttgac 231780ctgtcagagc aagtcttgtc aactgggaca cacccagcca tggtagcacc tgtatgcctt 231840caggcaacca tttgtggctg ttcaaatgta aatgtgttta agttacacaa aattagaaat 231900ttagttctgc ggtagtaaat aacagttaca cttcacacac acagcagtca ccatgttaca 231960tgggcacttg agaacagaac tgttagacct cactgtctat agatgggttg gggagggaca 232020ggggtcccct gagagtgaca cactgtctgt cactcattgc tgctggttcc actctacagg 232080tacagctgga taccttgcca ggaatgagcc tggtggcagg caaggcgctg agctctgccc 232140ggatgtcaga tgcagttctt agtcagtctt cactcatggg cagccaacag tttcaggatg 232200aggaagatga aggtaaggcc ttgcccatgg gcaccccggg gcagtcttct gtggaaattg 232260ttcactggct gaggtgctac gcacatgtgc acacgttagg ggctctggct tctgtcagca 232320cagggacatc acagtccaag ttcagtgctg gagcaggatg taccttcaac aatagccctc 232380tttcagcttc tccttagagg gcttcatatc cttgtagaac atttatttct atgtctttgt 232440agagccaggg tgggagacca tgatagagcc tggagacgga caggttcacc ctttctaagg 232500tccacaggca aagcctagga agtcttcatc ccaagtcttc acctgtgagg acatcaagag 232560agcagtgccc atgtcctggc ttgggtccta aggatgctgg cctcctgagg tttattagtg 232620tttattagtg tgatgctcaa gagaaagtgg cctggcctgg ctcctatagc actcactaca 232680gttatcctgc cccctaacca caagaaaagg ctcttgaagg gtttttcatc ttcaccccca 232740tatgtcagtt gctcatgagg gtggcgaaag gaaggggcag gtggtcagga agggtcccta 232800acatctgaag ggcctgctgt ggcctgagtc agagctggac tcaggctggg ttgctagaca 232860agacctcccc agcctcctct ctcctgtccc acttgctccc ccacctccac ctcacatgtt 232920ctttttggtc ttgcagaatg tggggtgagc ctgggccacg agcatccagg cctgggtgat 232980ggcagccagc atctcaactc ctctcgctat ccagctacgt gtgttacaga catcatgctc 233040agccacaagc acccagaggt ctccttcagc atggagcaag ccggcgtgta gcaagatatg 233100gaaagtgagt gccaccctgc tcaggcccag gcagccctgg ggcccagtgc cagcccctca 233160tagcctcaat gtgttcattc cttgtgtggt accccactaa acttgagcga cactcttttt 233220gtgcaacctt ccccgtctgc ctgtttcttc attaagtact gtgttagttt tgaggtgggc 233280tgaggacttt aatttaggga agaggtacat ggcccacctc atgttataga tgctatggct 233340ttaatgagat ccaagctaca gttgcagagg ccagaggcca atgacctttg gccagctctc 233400taaagctagt ctttacactc catcctgaag agtgaaaccc aaccagaaaa gggaaaaaga 233460gcagatccag tcaggctcct tcactggtgg tttttattgt ttggttttgg cattttggtg 233520ctaagcatag aacccagggc cgtgtaagcc ccagccccag cctcaacccc agccccagcc 233580ccagctggac tttttagggg gtaaacccat gctgaaaaga cagaaggctc accggccctg 233640ttggcctagc agaggtcaca tggatggtga gtactagtgg ctctatctct acagagacag 233700aacaacttga ggaaggtaaa gccacaggca gaggcatggc agagcctcag tgtggcaaga 233760ctcatacagt gacaccaaga aaagacttca ctgagagcct gcatgcctgt ggctcactga 233820cagagcaggg catggacaca tagggccctg agtcccagcc ctagtaccat tagaaagggt 233880ggggagaaag ggtgaagtca tgtccctctc cttcaaggtg ttcttgtctg cttcgtaggt 233940tcgagtacag ctccaggagg aggctgagtc tagaccaata gaccaacagt gtccaacagc 234000gccgcaccgc tcctccgtct gtgtctccac cacaacagtg cagctccttc ccctacacta 234060ggcaccagta ggccggcgct tgggttgcga gggacatttg ccatgtttgc ttgtatgacc 234120aaggcaccaa ggaaataaac aggaaatcag tgtcctccat ctttcatgag ccagcatctg 234180gtgtgcttga gttgagttct gctgttagga ggtttgcttg tgttttgttt tggaaatttt 234240cctttggaga taacttattc tctttgcctt gcccacccct acccccttga gcatattccc 234300ccccccctct cgttttcacc taccatttct gaggtagatg aaccagaggt tagtattcag 234360gggctgaacc tcaggaccct cccttccaca gcctgtctca gaccagactc tctccctctg 234420ccccggtcta ccaggaacaa atgctagacc ggagcttggt ggccaagaaa aggagaggag 234480ggggcagtgg cccctgcctt tggagattgg aacagaagaa aaaactgagc ctgggatctg 234540gcagaatctc ctgctggcct ctactagaat cctatctggc ccagcagaat tccatcagat 234600cccagcacag gccggatggc ctggtgctga aggtttgcca tccagaatag ctcagtgtag 234660gcccctctgg tgtgtggtcc agttcccctc cagtcaccaa ctggttcccc ctcatgagtg 234720aacacccagg agtggtgcac tgtgctgttt gtgtgatctc cattcttatt ctctactgcg 234780ttgtaaaaag ggccgttggt ccatttgtgt gcacacgtat gagtgtgtac atgcccttcc 234840ctgcggcctc tttgtcacag acaagaattg gaggctgtac tgggtctctt aaccttctcc 234900tctccccagc atcctcggcc cctatatttt aattcttgat catgtaggaa ttgtttttga 234960taaatgttga tattattgtt attattgtta ttattaataa agacaaaaag gattttgttt 235020atttgtttaa aagagaaatg tttaaccaga ttctgtgctg ttgaactgtg gcttgcaact 235080tctgtttgaa gtgttttcct tggctttggc atcgtgagcc actctcctgt gccagcagcg 235140gatgcagtgg cctcccttcc ccagccgaag cagcacatag gcagtggcta ctgtccttgt 235200cacttccttt ctctctgcag tcagaaacca aagtctgggg ccaaacgagg ccggcccggc 235260ttctcttctg gtcttcaaaa ccagagaggg atggtccctg ctctgactcc cagccctcat 235320ttctgagagc caggggccac caacctctga gtatgcacag aagaaagccg ctgcctgcca 235380tggcatctgg acattaccag cagggtccct agccatttta tccttccgca ccaaccctga 235440aagcttgcct ctttgaacaa atccattgtc tcttgaatgg atttgttttc aatgtgagag 235500cttggctggg gacctgtttc gtggtcccat gtcctccaat ttcccctcca cttcaggtgt 235560cactaattac tagccagaag atgtctgact cccaagaaag cttgacaagc cattgtcagc 235620ttccaagctc agtccagctc tgatactcca gccccgccct gctgctcccc acgctcagcc 235680tgacctgttt acgtggtact ggattgatgg aagagcaggc accaccctcc agagccaccc 235740agacgaaagc cagtgagcct attaaccgtg ccatcttgcg aactattttc aagatcacca 235800ttgctttata ttgtagtaac caatatgcgc agtatatgtt gaatgtatat gaatctactt 235860tgctatttct gttctttgaa aatgtaagaa gtatttttcc tttctcattt ctgttgatct 235920aaaaatgata ataataataa taatctccag attcaagttg ctaagagctt ttgtccttgt 235980ttgtgtggga catctacaac ttagtgactt ggttggactg taggaggttg tggaaataaa 236040aacctgatta tgtactaacg ctgatatgtt gtttgcctta cggttcagag tctaattgag 236100agaatcacat ggggtagggg cagaggtgac ccaggtgtct ggagaagtcc ccagacttag 236160ggtggggctt ggagcagcac attctcacct tgccttttgg cctctgaacc tggcaaatca 236220gactcacact gtgtccaggc atccgtgtgg actctaaccc ctcagaaggg acacatttgt 236280gtttgcagag ttgggtggga aaaggattcc ttggggtcct cagaaggaga gccttgcatt 236340gaccctggct ggaaccttta tgaggtggcc tccctcagct gtaggacaga agggcttttg 236400gtactgtttc

ccaatgtctg ttcctggatt ccaggtggag ggcagaattt ccaacagggc 236460cccaggctct gctgaaagag ctgaagtcac acgagaaaaa agatcaatga tctggggaat 236520acggagattt gtagaaagag tgacagaggc tggaaggatg gctcagtggt taagagcacc 236580aacttcctgc aactcacacc acacagaact ccaattccaa ggacccgaca tgcttcccag 236640gctcctgtgt acagataaca tacactcata cagatagaaa actctttagg cagggagagg 236700cgcatataag aaagcctttg aggctagaat tgcaatgaaa ctgctcagag tggaaggtgc 236760cttctcagcc cctggaggtc ttgaaggtgt acgcaccgca cagaggtgag ctggctttgg 236820aaccagtgat gggccctaag gactcccaaa aggtcaggac acctttcact gatgctgtgt 236880ctgcaaagtt tttggctaga ggtcgggtag gtacccagag gcagccagat tgactctggg 236940atctgaacct ggacatctct gccctccaag aaggaaagaa agacttcctg actttgtact 237000ggagccaggc cccacccact taaccacgga gactttagtc tcctgagcag gaggcaggaa 237060catcattcag agctgatgac agtgtccttc caaacaggaa tcactgggga tccagtctgc 237120ttagggagac cctccccagc cctggctccc cattcctgct agcactaccc cttgttgggt 237180acatattccc agaaggggct ggcaccattg aaggcctgcg cctttctgtg accctttgcc 237240agtctgagaa gctttggtgt atcaatgtta ttcatttagt aaatgtttgg tgtctctggt 237300gccagtccca ctaaaggaca gagtccttgc actgccctgt gcaggtgatc tgtgctgaga 237360cagaagttgt gaaatgggct acattcgctg ctgctgaagc taccagcact cccgggatcc 237420caggccagca cttgctggtc caatccagga gtcactttgg aggttcccaa gatgaggggg 237480tgaagggtag tgagagaaat gccaatgata ggcactatct tcactccctg gtcacccaac 237540acctctaaca gcagccaacc tgtctcaatc tgtgatgagc ccatttgact cttgatctgt 237600ccagaaagac ttcctgttca gaacagtaag tagctattta gactagttta aaccaaatgt 237660ctcctgataa agtaagtcac tagcaagtaa gtatggggtg tgggggaggg agcatggagc 237720atacacctat aaccttagca tagaaagcca aggcaggagg attaccacaa gtttgagacc 237780agcctggact atataccaag acaggagcac acttattgct cagtcaggtt gtcccagagt 237840cgtctaccgt gatgtcatgg attccagtcc ccaagccatg tccctggaga gagtacaaag 237900gctttggaag tgaagaaatc accatcagag aactagaggc cggacccgat ccctagcccc 237960ttgggcactg tctcaaagct ggggggcaag aatgttctta gcatctctga gctgccgttt 238020tttctgacag gcaaactacc aagggggtga acatggcgtc cagccttaca ggggtgtctc 238080aagtttactt taaatgagtt gcggagcaaa tgttaggttc aattaggatt agccagcacg 238140gtttcagtaa cgagccagaa agaaagatag gactgtggga agcatcctgg aaaccaacag 238200acacccccac ccccacccca cccccaaaag acctaaaaaa ctactcctgt ggaggccacc 238260tccctctgag tgctcccaca gccctcgggg gctggtttca acctggaaaa gatgtcctgc 238320atcacagcac ctgcaagcca gacacctgcc ttaccccatt tttgctgcca gcaactgggg 238380tcacaggcta tggcctagta acagaatgtg ttcgtggcag tgacctcagg agtctgggat 238440gcagaacact tgccatttgc tgttggtcac ctttcagcag ttttgcttga aatgtgttca 238500gacttcacag acaacacaac acaaataaaa ctctggagtt cagaagctca aatcatggct 238560aaaagcatgg gattgtcaga gtgatattct gtctgaaaac tctttcattc agagaaaacc 238620tgcattttct cttctctctt ccagcaccca tggatgggta gccgaaaccc gcttggctca 238680tctgcacact cagattgctt ggtctctatt tctgttctca agtttctcca tggttttggc 238740ccttctgcct cctctagcat attgagagac ccttgtgatt actgtgccct ctggatatcc 238800caggctaccc ttgagttcaa gtcccctaat cacatttgcc atgttagaca ccacgcacag 238860gttcctggac ttagtaagtg cacacacatc ttttccattt tgcttcccat acagtgactg 238920ggatcataag gaagctgtgg catgctctgt cattatcttt ccttagcaga taagagaccc 238980agggacggag aggttatgta acctgtccgg gtcacctgga gaataagttg cagatcccag 239040aaccaaggtt ctctctggtt gcaaagcctc ctgggatggg agcagcctgg aatcccagcc 239100actccctgaa ctggtagaca gattccaagc tggtttctag ggagttgggg agtttcctag 239160ggagatgggc agagtacatt cctgccctgg gtcccaaatg cagagacctg ggcgcaaggt 239220ccatccttgc ctctaaggct gagagataaa ggacacagca aattccttcc actctcaaca 239280ggcccttatc tagaaggaag ttcatggtag aacctcaaat agatggtctc aaaagttcct 239340cttagcttga aaactcactt tccataatgg gattttagag atgatctgtg gtttttgttt 239400gtttgtttgt tttgttttgt tttgttttgt ttttagatat tctcatatag cccaggctga 239460cttcaaactt gctatatggg caaggctgtg gttttgaact ctcaaccctc tgaccttcca 239520agtgcaaaaa ctggccaccg tactcagccc actctacaca gctacccagc tctcccatcc 239580tggcaagtga gcctaggccc tcaccactct gggaagacat atcgtcactg aactacatga 239640ccccttttag tggtcatggt atatggtgag gttttctctg gaagggaggc tgaataacag 239700catctacaca taacaaaaac attggcaact ggggctggag agatggctca gcggtttaag 239760accactagct gctcttccag aggtcctgag ttcaattccc agaaaccata tggtggctca 239820caaccatctg taaaggaatc tgattccatc ttctggtgtg tctgaagaca gagcgctcat 239880gtacctaaga taaggaagaa aggaagggag ggaaggaggg agggagacaa ggaaggagct 239940aacaactcca tgttggcctc tgtcatttct tctgaagcat cagaggggtc ttcatcctca 240000gcttggggat atgagcctgt ctgcatctca ctcaggctga cacctcagtt gtttttggat 240060ttagggttgg gaggctggct aaacatgtgg catctggaaa gacaaatccc caaacctggg 240120ggaccagggt gaggtgaaag ggggaacccc ttggaaagca aaaaaaaaac tttggggtgg 240180ggctgtgagt gaaggagccc acaattcctc cctccctccc ttgttccagg ctcagagggc 240240actagcaaca cagaggagtc caggagtggg ctccatgaga ctatcttgaa gaagacatac 240300acctcgggat ggtgcaactg ccttacccct ggggacccct ggagtctgca gccaccccct 240360gctgccccca cctgaaccct tgatcccagc tcggcagccc ccgcagtttc ctgtttgccc 240420actctgtttg cccagcctca ggaacagagc tgatccttga actctaagtt ccacatcgcc 240480agcaaaagta agcagtggca gggccaggct gagcttatca gtctcccagc ccagcccctg 240540cccacacaca tatatagacc agggaagaag agctggacac ccagactgtc ggagagctcc 240600ggggaggtaa gtgctgctac ctgccttcgg tggctctggc tccatagcgc ctcccagttg 240660atgctccact gtccaaatca ataccgtgga tatctcgcac ctttagccat tctagccaat 240720gcttccatgg gcttgaattg tgtgtggagc ctttccatga caatgcctcc cttcagccca 240780gccctcctcc tccttcttct tcttcaggtc acccacaccc ttcaggatga aagctgtggt 240840gctggccgtg gctctggtct tcctgacagg taggtgcctc ttgacctgcg tgggactacc 240900ttcctgggca cagagaacag aattcccact gttctcttcc ctgactccga gtctaaccta 240960acatgggtct cccctccatc cccagggagc caggcttggc acgtatggca gcaagatgaa 241020ccccagtccc aatgggacaa agtgaaggat ttcgctaatg tgtatgtgga tgcggtcaaa 241080gacagcggca gagactatgt gtcccagttt gaatcctcct ccttgggcca acagctgaag 241140taaggaaaga cctagcgtgg ggcctggagc aggtcaaggg ctgcccatcc agggtgggca 241200gagagaccag tgagaagatg ctggaactga gctggctagc ccttcacggg ctttcctacc 241260agctgggcac catggcaggt tccagtggag gactagggat gggattcatc tggctgttgg 241320gtaaccacag cccctcattc agcctatgag tgccaaatcc cttttccttg gtaaccccca 241380gtactggtca gacagcaccc aaaacaaaac aaaacaaaac aaaaaacaaa aaacgggact 241440ggccttgtaa ccagcaccga ccacatctgt gttcttggtg ccttccgcca tgtgtacaga 241500ggaatgtaaa gggaaggcag tgagttagag ggttgcatgt tcggggaaac taggaccata 241560gcaactgcac attaggggac aggtggcacc cagctatcat gtgcatggat ctgcagacca 241620ggggcagcgc atgatgcctg ggctcgtctc tcagccgctc tcttccccct ctagcctgaa 241680tctcctggaa aactgggaca ctctgggttc aaccgttagt cagctgcagg aacggctggg 241740cccattgact cgggacttct gggataacct ggagaaagaa acagattggg tgagacagga 241800gatgaacaag gacctagagg aagtgaaaca gaaggtgcag ccctacctgg acgaattcca 241860gaagaaatgg aaagaggatg tggagctcta ccgccagaag gtggcgcctc tgggcgccga 241920gctgcaggag agcgcgcgcc agaagctgca ggagctgcaa gggagactgt cccctgtggc 241980tgaggaattt cgcgaccgca tgcgcacaca cgtagactct ctgcgcacac agctagcgcc 242040ccacagcgaa cagatgcgcg agagcctggc ccagcgcctg gctgagctca agagcaaccc 242100taccttgaac gagtaccaca ccagggccaa aacccacctg aagacacttg gcgagaaagc 242160cagacctgcg ctggaggacc tgcgccatag tctgatgccc atgctggaga cgcttaagac 242220ccaagtccag agtgtgatcg acaaggccag cgagactctg actgcccagt gaggtgcccg 242280cttccactcc ccacccccgc attggctttc ttacaataaa cctttccaaa atggaatagc 242340ttctttcttt gggggacata gggcgggcgc taaggggaca tcaagggacg tgagaacatg 242400gtgccgcact ggggattcct ttgtacgcga ctactcagct cttaacgctc actcaagctg 242460ggcacctggc tggttcaggg tatgagacag aatcccttcc taagaaggct ccaagggaaa 242520tggatggcca ggcaaagcaa caaggaaccc caaaagggcc ccacagaggc tcagaacact 242580agtgctgtaa accaggatga gagtgtctgg caataggact ggctttgaga ccgctggttt 242640cttgtgctag ccgagtttcc aggtggggtc cagagggtgc aggacgggag cctcttccac 242700tccctagagc caggattcct tcaggagcca ggaagattgg agttattggg tgggtgctaa 242760taaaaagcct ggagctgaac ccgatctcgg taaacaattc cctttgatct ttccctccgg 242820tgcacccaca cagtgtgctg aaagggtcaa ggctgcagct actctacctg acagttggat 242880acaaaaacaa acaaacaaac aaacacccct ctccatgatg ctggtgacca cacatttact 242940agagaggcct tcatatccat ctctaaatcc tgaaggatgg cctttaagtc cacaccagtt 243000ggggaagctg agctgggagg tctgaagtta gtcactgcag aaaaggtggg tcaggatgtg 243060ccgttccatt ttaaaacaaa acaaaaacaa aacaaacaaa caaaaaaacc ccttttatta 243120catggggctg gtaaggtggc ttatcaggta gaggcacttt cggaagtctg agaacctgag 243180ttcagtgctt agaagacaca cacacacaca cacaccccac aaatataaat aaaggtatat 243240aaaatattta aatttttatt agacttattt agtgtgtgtg tgtgtgtgag tgtgtgtgtg 243300tgtgagtgtg tgtgtgtgtg ttttcacgtg ccacagaaca tgtgtggaga ttagaggaca 243360atttgcaggt atctgttctc ttttccacca tataggtccc aaagatcaaa aactcaggtc 243420accaggatta gtgtagcgag cgtctctact agctaagcca tcttgccagt tcttgtttgt 243480atttatctac tagctaagcc atcttgccag tccttgtttg tatttatttt atttggagac 243540agtgtctcca ctagcccagg ctagactcag atgagctctg atctttctgc cccatctccc 243600aagtgctggg ggtttcaagg atggtccacc actgttactc tgtgctgaga caaacccaga 243660gctctgtgca tgcaaggcaa gccctctacc acctgagctg cctgccaagc ccagactgtt 243720cattgtatag acttgcccgc tatggagtgt aaccaagcac tcatgttcac agggttgagg 243780atgtctgcag aagcactctc acaccaacag ccttcctctc atctgcccct cttcatgcct 243840gtgttggtat gcaggtggtc tccatccatc cccggtgtat cttcatgagc ccctggctga 243900aggggtgagt ttttaaatcc acaatcatgc agagccacag cagacattaa accaggtttg 243960ggtaggaaag tttcccggtg tggacaatga gacttctggc tcttggtgga caggaagaga 244020aacccctatt ggatttccag agagaacata gtccagtctg ctggctgtgc caatgcagtc 244080tggaaaggag cttccaacac ctggcagagg cttttccggt gccagcaacg tggaaaatgg 244140aaagctggcc ctgcagtgtg ttttctcctg atccggggca gccactggca ctctctgaac 244200attagtcttt tactctataa tgtgcaagac ctgttttgtg acactgggtc tctttgaagt 244260gagtgtttgg gggtggcagg aacttagcaa gcagcccttt tgggattctg aggacactgg 244320gtctccattc cagtctagaa ggtcacctag tttagctatc acagtgagga ctgtttacaa 244380ggggtgtgcg catgcggcac ctcatgcttt tagacactac catttcattt ctacaacaaa 244440ccttcagggg aggggcaggt cctacccact ttattcataa gaaatggaag ccgctacact 244500gcagtggggt tgagacttat ccgaccaccc tcccaatctg cctccttcct ttccgcctgt 244560ttaaaggggc caggcagcct gggctgtgca aaccagctgc acggggttgg tgatcatatc 244620tgaccttaag cctggagatg acataagact gctagctaga cagatactac actccagcca 244680cgtcgtccac gttgtcctgc ccttgacagg tttatcacag attgtaactg caaatctgtc 244740gttacctgat ttgatctttc ccattaagtc aagcagggaa gcggagttag tcccacgtac 244800aaaggaagaa gcaccccaga gaccactaaa gaggtggatg acctacagcc ccaggtccac 244860cccttcaaca ggcctagctc atccccatgc ccagacgtcc aggcacatgg gacacccact 244920aacagcagct tcttatccag ctttattagg gacagcatgt ttaggtgaga tctagggagg 244980ggtgaagaca tgagaacata ctttcccctt aaagcaacct tcaggggcca cctggcaggc 245040ctggaggggg gcagcaggat ggaggaacag gcacatctgc aacacagaag tctcacgact 245100caatagctgg agttggttgg tcctcagggt tagaatccca gaagccggtg aacttgtcag 245160taaacttgct ccagtagcct ttcagggatc tgaagtgatt gtccatccag cccctggagg 245220ttaaagcagt aagaggcatc cataggagag agggagagag agagagaggg agagagagag 245280agagagagag agagagagag agagagagag agagaggtgt gtgtgtgtgt gtgtgtgtgt 245340gtgtgtgtgt gtgtgtgtgt gtgtgagaaa agctcccccg tgggcagcta gacccaggac 245400tgcacgcagg gctccactgg ctctctcagc caccagcacc accttgccta tcacacccca 245460aaatcacctg cagctaccta agaaacccca ccttcatcaa ggttggatca gaacctcagt 245520gccttccatc caatctcctt tcaggccgga ggcaccaagc accggggagc tggatactaa 245580ggtcagctac aatgcagctg cctgtatagg ccatacaaat cactcccttg aggtacccca 245640tccctggctc taagtgggtg aggcagaggc agggccagtc tctcatttga aacattactg 245700aatgacttct aagctgtgtg atctaggcca ggttatctaa ctctttatca tcagcaaaac 245760cgagtatcta atatcctccc cacagcctgt tctggggata gagactgtca acatcttttg 245820ctacataggc aactaaacta tcaaatgtca gccaaagcca ttggccaggg ggtgatattt 245880catagtgggc aagatgccta cctcagactg agcccaggga tagaaacagg gcagactccc 245940accctctaac ccccaacttc ccacctcact tcccaccact accccctgca tacaggcaga 246000aaccagagca ggtaggcctc ccatcgtgtg cgcaggaccc agaggagaaa acctgcagcg 246060aggctaaatg cccctcaggc tgctctgagg aaaagcagag aagataggag aaggctgctt 246120gccctgcctg tcatcttggt cacagtaccg aaaggcctca tgagggacca gaggctcggg 246180caggtcccag gccctggtaa acttggtgga ccacagtctc tgtgctaggg tctcagcacc 246240agggcatgag aggagaagac acaggaaagg aggccagtgt gcctacctgg ccaccacagc 246300tatatcggac tcctgcacgc tacttagcgc atcctggacc gtcttggagg cttgttccat 246360gtagccctgt acagagccca gcagcaagga tccctctacc tcttcagctc ctgcgagaga 246420gcagagttgg gccacgccag ccctcagctc ctgcccagcc accacttctc agggcagggc 246480cccctgacca gctcccactg aaacccagcc ccactcccac catcttacgg gcagatgcca 246540ggagagccaa gagggccaca gtgaggagcg tccggggctg catggcacct acgtacctgt 246600gggaaagcat ctcgtgagaa ggtactgtgg atctgccaca tctaggtcct ccccttgaga 246660atgccatgac tcctgggtca tgaatcccaa gcctttctcc cactgatatt agatagagaa 246720ctacagtaga cccagccccc caagtggggt ggaaggtgga gtatagaaat actctgcaga 246780acgggacatc tactctaata tgctgagaaa caataggttt cttttcctcg ctaggactca 246840gttttttcag tcctgggtag gcatggatac caaaggcttc taatagctca gagcaagcta 246900aacaagggac agcatgaccc aattgcaggc agctctgcca ctacccagtg caaggctttt 246960ggcccatagc ctccctttcc ccagcttcta gcccccccca caccaggaac ccaagggtgg 247020agaccatgag tcccaagcct tctgtgggct agatggctgg gtggtgagag cttctccctc 247080cagctctttg tttcttccct tccttcctct cctccccagg ggcattacct ggagtagcta 247140gctgcttcta gggataaaac tgagcaggca agcggggagg gctgtgacct gttttatatt 247200ggctccagga tgggacagcg ggcacagaag gcccagtgag ctgggcaaag gtcacctgct 247260gagcagtcca gaccagagcc tgaggcagga aggccatgca gccatctgcc agaggagttg 247320agaaatccct ctgagattgc ccatgctttt cacggccacc tccgccacca agggatcagc 247380taccggctca cctagatgag gtcggtgaga caggaaaaga caggggacaa gccttggacc 247440tcaggcctgc tttacagcct agagccagtg acaggctcct tcacagaagc cacctgaaag 247500ccttccagta gaatggaatg gggaatctgt ggtgccactg tggagcaacg gaggaagtgg 247560ggtgggaggg ggcagagtga aggttacatg cccccacact gacctccacc tgtgatccca 247620acagtctcct ctgccgtagc tgggcaggga ttagtgagag aaggacaaag gtcacatgga 247680acccaaggcc ttgcccctcc ccaactcctc cacaggcctc ctcttgccct ctggacctat 247740cgttagtaat cctgagatag gagcagggcc catggctggg tgatatctga cccctacatg 247800agccctatct atgagaacct gcaagcagca aggtatagac agggcattca caaagaaggt 247860gaggatgaag gaggcgctct ggggcaacta agatagtccc ctgagctagt actgagaccc 247920acagcctaga cagcaacaga ggacatcatc ttctggatgc agactttgga gccaagaaat 247980cctaggttta gggttttttt tggttggttg gttggttggt tgattttttt tttctgagac 248040aaggtttctc tgtgtggccc tggctgtcct ggaactcact cttgtagaca aggttggctt 248100tgaactcaga aatctgcttg cctctgcctc ctgagtgcta ggattatagg catgagccac 248160tacctgccaa aatcctaggt ttaaatgcta cattcgggac tagagaaata ggccaattgg 248220acaaaatgct cgtcttgtaa gtaggaagac catagttcaa tccccaaacc ccatggacag 248280cataagtgca gtagtgtcca cttgtaatcc caagcactgg ggacagggag ataggtagca 248340ggccaggagt tgatgtccca ccagcctagc ttcttcagtg agccccagat accactgaga 248400gactctgtct cataaggcaa ggtaaacagc tcttgaaaaa tgatacctga gccaggcagt 248460ggtggcgcat gcctttaatc ccagcacttg ggaggcagag gcaggtggat ttctgagttc 248520gaggccagct tgggctacag agtgagttcc aggatagcca gggctacaca gagaaaccct 248580gtctcaaaaa accgaaaaag aaaaaaaaag tagaaagtag aaaaatgata cctgaaactg 248640acctgtggcc cccacagttg tgcacatcac acatgcctat tgacctgcac aaacgcctaa 248700gcaactgacg cctttgaggc tgacatgtag ggtctgtggt cagaagttaa cgggatggga 248760cactgacttc ccagcgtgtg gagaaacaca cagcagctgg cccagggaag gactcagttt 248820ccctacctcc caaacactga cagcccagcc agagacagca ggcagtttcc atgcagaaac 248880agggcttgct gcaggcagca acctctctga atgaaatctc agcctgagag gagcctggct 248940ttcccctcag aactcttaat ctctgtcttt ggctgagaca cgaagctcat ctatcagaaa 249000tacattcttt tgatataaga ctgttgtctg gggtagctgt gatagtgttt gcttaaaaga 249060ccagcactca ggaaaatagt gttagaggtc agcctggact aaataatgtg actgtctcag 249120aacaactcaa aacagtttgt ttcctgttaa aatgtaagtt gtccgaatta tgtagcttag 249180acattactaa ctccgtttat ggctaaaaac caaaggccca gtggggtaga gactgctcaa 249240cacatgccac ccagcagtgg agaagctggg tctcagcacc tgagctggtc ctcagcaccc 249300atttataccc aagcttcctc tgagaaaagt ccctggcgaa gctgagaggg aacaaaggtg 249360gtaccagggc ggggcactgt gtgtcgtggt ggagggagat gccatgttct tttttcttct 249420cagcctgttt cttgttccta ggcaaagggc acagcaggga tcagatccca atctggggtg 249480tgatatcaag tctttggccc tgagaatgat ttaacaggct gactgggtca ttgtccattg 249540gtagggaggg tctgggaagc ctgtcacctc ccctgtatgt agaacatggt cactgaatca 249600agaaaagtca aaagatgcca atagagagtc ccagccatgc gatacttagc tgcacagaaa 249660acttaggtgt atgaatccca cagagtggtt ggcaggcagg tgataccttg gaacctgggc 249720tctgggactt agtcccttcc aaaaaccctt gtaccctctc agccatagga gccttatggt 249780ggcctcttgg tggctttctg gcctagggtc ctctggggaa aggacagtgt tcttgactta 249840cagacccagt cccatggagg aaggtagcag tggggggggg ggggagtttc ttaatttctt 249900ttcctgttgc tatgatacaa tatcccgacg aaaacaaagt gaggagttat cctggctcac 249960acatggggag ccatggtggc aggaacacga agtagccagt cacatcacgt ttacaactaa 250020aagcaaagag ccatgcaaag ccagtcctca gctcggctcg ctttcttcat tttgtgtggt 250080ctgggatcct tcatccaaca accccccccc accccctcag gtgattccag attctgtcaa 250140attgacagtt aaaactaatc accacactgt ggaagatgtc ttgaacattt gggctgagaa 250200cagccctcag gagtggcttg ccagccctcc tctgtttggc taacagcagg ttgggtggat 250260gagagcccca gggctctact tggatcctat aggcagatgg atggtgcaca gctaccaatc 250320agccttacta gccatgagct ccgtgtgagc acaggcagag actgagggtc agacagagac 250380gtgagttgcc aagaccactt gaccctctgt gcccgaggct ggcataaggt ggtctgctct 250440ttttccccag aagctgacac atagggttct cattttgcct caccctctat gcaaatcacc 250500agcagatgca tatgaagtat gccccagtct tggagaagtc aatatgatga tttatgacgc 250560gccaggaggc aatacaacct ccctgtaggg gctggtgcat acgtgagtgc tagccacaca 250620tgtgtgtacg atgacccagt ctcttagctc agagccaact tctgaacccc tggacaactc 250680agatatctat gcatatctga cagcccgtca ctggaaacca cccttgaaca aacttaggta 250740acacaggtct tctcagagag ggtatgagtg ggcaagcacc tctggacctg tcccacgctg 250800ctggcacttg agagactcag tctgcaggtt caggaggtgg aactgtagct gagatgtcct 250860acgtgactca tcccccatca gttctgacct ttgggcacag cacaggctgg accaggcatg 250920attaggtaaa gcaagatgag gaccaatggg tcaatggtac acagagagaa accgtacata 250980gctaatattc aacgaggaac ctaaggggag ccagactatc tcacagaatc ttcacaacga 251040agactcatcc aagaccacac agcacagact gtgtgggatc cacgtgcaaa cacagggagt 251100cgactccaga gcctgaactc agaaaacatt gaaagaccac caagatgact cagtgaacaa 251160agtcacccag cctgatgaca tcagttaaaa ccctagaacc caaatggtag gatgagagaa 251220caaacctctc cagttctcat ctgacctcta tacatgtatt gtgatgtata catactcacc 251280cctaccaaat attagtaaat aaataacatt atcactatca tcattatttt tgtttgtttt 251340gttttgtttt gttttgtttt gttttgtttt gttttgtttt gagacagggt ttctctatgt 251400agccctagct tcctggaact cactttgtag actatgctgt ccttgaactc acaaagatcc 251460accaatctcc

acctcgtgtg tgctgggatt aaaggcatgt gccatcacac ctggcataaa 251520ttaattttct taaactcact aagcatggct gtgctaggaa ggaatgagct cggggaagct 251580cgagccctgt ggggagccat gcagtgcagt ggggcccagc agaggagcac aggtatccag 251640ctgtcttcag tcccatgaga caagctaatc tggacacatt ttaaaaaatg gatggcaaca 251700cagcaaatca gactgggcac aatcgtggtc tattctaatg gctgtcattt cacaaatgct 251760gtcttgtgga tggcagtcaa tgggacagta tgatggatgc cctcatctag tccctggtgt 251820ggtccactga ggctccacac tgaccacagc ctggcatctt gcctgtggat atctgctgca 251880attgtatgtg tggacacatg tggagtctca gtaggagacc tcaaaaaact cactttccac 251940agcagtgtct gtcaccttct gtgggggggg ggggggtggg aagagagaga gagggagaga 252000gggagagaga gagggagaga gagagaggag tcactctgca tggctcttgc atatggctga 252060gaacagtggg gcagcaatca agccttagcc agccctgctc tctcactgtt gcctctagcc 252120cacttggtga ccctctgagg gaaagggtgg ctctccctct gccactgtca ggagaggatc 252180aggttctctc cttccttcct gtgctgatgc acacagaaaa tcattgtcat taatttcagc 252240ccttactctg ggctaagctc cctgcagcca tctcacaagt accacctaat ttaatgtaac 252300aaactacaca ttgttcaaaa gagaaacttg aagcttcatg ataactggac ggaggtgagc 252360cagcttgaca gtcatgagat acaaagccca ctatgattaa ctcctttgat cctgggttct 252420gatcctctcc tgaccaaggg tatcacagac acctcaactg aggctcactg tctgctgcag 252480ccctatgcca tctctgggcc tggtaccatc tctgtagctg atgttctgag acaaagttca 252540ggttggtggc agctgtcaga ctggtggctg tctcactggg gtggaaagag gagacctgga 252600ccttgttctc tcagactggc acagacccag ggctgccaac cgggcctctg gggcctcagt 252660tctgttcagg gactccccta gactcccagg ctcattcctc ctgaagtttc tggctatcct 252720tcccagcctc ttggacaggg tggagccaac tcaagaagac tgcttccctc tgctgcctgt 252780gtgctgtcag cttccacgtt gtcttagggc cactaaagtc caagaggcct cctgggagtg 252840tgtcaccttc caacgtggag tcacactggg gaggaggcgg ggagagaggg ctggaggggc 252900tttaaatgag tggctggcct tgcctgcagt caatctgcac agggacacag gtacaccgtt 252960tcttctgact ccgggaaaca tccagtgtag ccgaaactgt cccagcccag tgaggagccc 253020aggatgttcc tgaaggctgc ggtgctgacc ctggccctgg tggccatcac cggtgagtag 253080acactgcacc tgggaggcag caagaaaagc cagctctaga actggcggac agctcggggt 253140ggccttgtat ttgcccagca gctcatagga gaacaggcct ttgttctccc tggcacttgt 253200gctccctggg ttatcccagg gatggggcaa tggtttgggt tatccaaact ccaacattat 253260ccagctcaga gctgaggcag aggggccagg agagagatga tcctcataaa gttgccttct 253320gctctctctc tgcccaggca cccgggctga ggtcacttcg gaccaggtgg ccaatgtggt 253380gtgggattac tttacccagc taagcaacaa tgccaaggag gctgtagaac agtttcagaa 253440gacggatgtc actcagcagc tcaggtaagt gcgatcagtc tacaaggcga ggcttgaagc 253500ccatagctga cctcagaggt gggacactgg ctcctggagt tcttctgttc tcactaaagg 253560agtcttgcct ccctggacct agattgtccc tgtaaacagg aaagctggac ccagggatcc 253620atccctatag ttccttccta tctgcatgtg ctagagtttg gtaaatagcc aaatcccatc 253680acaaactctt agcaggaaga ggaaagagac gtgtcctgta aggacatgag gttctgggca 253740aacaggggaa ggagggctgg tattttgggg gacccactga gcacatgcaa ggacatcaga 253800ccctgtgcca ctggaagaca tgtgttgcac aatgtgaccc tgggagggga gtgatgccaa 253860agtccagccc cattctttat ccacaggtaa ggaaagcaaa cttctcaagt cacatggtgg 253920gcagataagg atgcagaggc tcagcagaag ccttgcagat aaaactccaa agtcaaacta 253980atcctaggag atttcctgaa agcatgacct accccagggg agggtcaaag ggacaagacg 254040gaggtctctg ttcccatgga cacactcctg acctaagcag gtgtatagag ctgagtgttc 254100tacaagcgta tctaatggtg cttccttgtc tccatccttt ccctgaagta ccctcttcca 254160ggacaaactt ggggatgcta gtacgtatgc tgatggggtg cacaacaagc tggtgccctt 254220tgtcgtacag ctgagtgggc atctagccca ggaaactgag agggtgaagg aagagatcaa 254280gaaggagctg gaggacctac gtgaccgcat gatgccccat gccaacaaag taacccagac 254340gttcggggag aacatgcaga agttgcagga gcacctgaag ccctatgccg tggacctgca 254400agatcagatc aacacacaga cccaggaaat gaagctccag ctgaccccat acatccagcg 254460catgcagacc acgatcaagg agaatgtgga caacctgcac acctcgatga tgccccttgc 254520caccaactta aaggacaagt ttaacaggaa tatggaagag ctcaaggggc acctaacccc 254580ccgtgccaac gagctgaagg ccacgatcga ccagaacctg gaggatctgc gccgcagcct 254640ggcccctctg acggtgggcg tgcaggagaa actcaaccat cagatggagg gcctggcctt 254700ccagatgaag aagaacgcgg aggagctcca gaccaaggtc tccgcaaaaa tcgaccagct 254760gcagaagaat ctggccccgc tggtggaaga cgtgcagagc aaggtgaagg gcaacacgga 254820agggctgcag aagtctctgg aagacctgaa caggcagctg gagcagcagg tggaggagtt 254880ccgacgcact gtggagccca tgggagagat gttcaacaag gctctggtgc agcagctgga 254940acagttcaga cagcagctgg gtcccaattc gggggaggtg gaaagccact tgagcttcct 255000ggagaagagc ctgagggaga aggtcaactc ctttatgagc accctggaaa aaaaggggag 255060cccagaccag cctcaagccc tccccctccc ggagcaggcc caggagcagg ctcaggagca 255120ggctcaggag caggtgcagc ccaaacctct ggagagctga gctgtccctg gtgccctcag 255180cccatcacag cagcagacac ctgtcctgcc ccaccacctg tctgtcactc tgtccccagg 255240cacttcttgt accagcttga ggacacatgt cctgtgggag gtgaagccac atctcgctac 255300tcaataaagc aactgagaaa ttagccccat cgggttgccc tgtgattcct tgggggctgg 255360cctgacgtag ggaggaaatc aaggcatctg gctggggaca tgggggtgag gggtggggcc 255420ggtaatgagc cttcgggtgg gtcaggggtt ggtgtgctag agaggagaat gcagaaaaga 255480cgccagtcta tcagaaaaca aaacacgcat cccgttcaca gcctgcagaa aacaaaacac 255540acatcccgtt cacagcctgc aaacagttat caaacgccct tgaggttcca gtcaagattc 255600tgagcactag tcaaaacagc ctttgtggca ttgtgtgcct gaagaccggg cttggtgttg 255660acagggacac ctggagggca gcaagaccca gtccctgacc ttgaagagtt tactgcacgg 255720caagtccctt cttgaacatg cctgtgcctt tgaacttatt tatacaaaag taagccactc 255780tgtcccactg taaagtactc atgtttgccc cggagttttc tccacctatg gataaatctg 255840ttgactccag agctgtataa tagatgccac gggatatgaa cgagccttgg cagccaaggt 255900ggaacaggct ttgcccagga gcctcctgat gggtggcttg cctgtctatg acaaagctgg 255960tggtcgctgg ggcaaagaaa tgaacccatc agaggacccc agtggtttta tggggacagc 256020cagccacagg aggctgcagt ctctggaatg aattttccct gcccttggag acttagagcc 256080tccctaagga gggaatctca gagtagagta gttcagggat tccttccaaa agatttcaca 256140tactggctgt agcctctgcc tcaggtctgc aagtcaaacc cctctgcctc agaatgggta 256200aagttcccct gggatggact ggaacatgct gtgggtggtc ccagctgctc tggccttctc 256260cctcccttcc aaggcgtcct ctgtgtgtca taagcccaga cctgggtttg catcctagtt 256320tcctgatact tctactgggt gatttggagc aggtaacaaa acctgttttg gaaaggaaaa 256380atgaggaacc ttgggccaga gttattcaga aggtctgatg gtgccaagca cttaatataa 256440ccatcaaggc aatgtaaata ctaaatgtgt gtgtgtgtgt gtgtgtgtgt gtgtgcgcgc 256500gagcacacgc gctcacagga tcacgcgcgc gctgtgctgc tctaatgtag cagacaccta 256560ggaaatgtcc tcctctgctc acatctccag accccagatc caggccacca aatgctttga 256620gtctccccag cccctgctaa cctcagctac atgggcccaa ctgtcacagc tacttacact 256680tactaccccg agcagggaag acccaggtga ccacatccta ctagggaaga cccaggtgac 256740caggggacca cagtcgtcaa ggatgcatgg cagcaggagt ctggctgaat ggctttaaga 256800aatgcttgct tagagaacca gaggcactac aaggaggcag gacaagggct ggttggtgtc 256860ctgagctgtt ctggaagctt ctggtagatc agttaggtga gctctgtctc tcaaagcctc 256920tgagtctccc agtctcttgt tcatagtgca aatcccgagt gggacccctg agttctttcc 256980tgctgtcttc aggactgtta aacacatctc ccaggggcct gccttactcc tggtttctct 257040gaatgagggt atacttgctt actttctctt gagccaggat gtcactccta accttcagtc 257100ttttaggtca cagcttccag gtcacagcat ttgcttttgg ctgctgtaat agactgggtg 257160ttcctgtgaa cacactgtgg tgcatgcatg tgtgcgtgcg tgcgtgcgtg cgtgcgtgcg 257220tgcgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtatg tatctgggtg 257280gatgagtgca tgtataggtc agaggataac cctcaagaaa accatttgct tcctttggga 257340cagggtctct tattagcctg gagctcacca attagggtag acaggctgaa cagtgagaga 257400caaggaccca cttgtccacg cctccctgaa atgaatgtat tccaaccatc aatgtaaagt 257460taggtatggt tgcacatgcc tttaattcca gcactcagaa gacagaagca agtagatcac 257520tatgagttca aagccagcct ggcctacatg gggaattcca ggctagccag ggctatataa 257580tgagacctta tcacaaatga aaacaaataa acaaacaaaa gagactgcgt tagattggtt 257640tattttacct cctattgctg cggttctgag gttttgtctc ttccatggtt aaacaatttt 257700atttgcagtt acttttgagt tctgagctca ttgccattaa gtaataacac ataaataaac 257760catctgtgac ccctaggtct tttcaaatgt cctatgaaga aaacttccct caagattcag 257820ctggctcaca gccagaaatg ataatgaagg acttttgcaa cttaatcctc aacccagtac 257880caagtatccc taccctctac cagtagatgg gccccagccc caggacaggt agaaaatgac 257940ccagaaacca cagtcagtgc aaagagacac actagtcaaa ttgagaagag agcaccagct 258000tagaggagaa ctgagagaca ctggacttaa gggaggtcag agagaagcac agagcactct 258060gctgccacag ccctgcagtt cctgtcatgg acaggtcctt ctgagagtgc cagaggagaa 258120aacaactgca tccaggaggt gacagctcac actcactcag aggcaggtag gtctctgtga 258180gttcaaagtc agcctggtct atagagtgag atccaggaca gccagaacta tactgagaaa 258240ccctgcttcc cccacccccc acccaccaaa aaaaaaaaaa aaaggaaaaa gaaaaagaaa 258300acaatcattg caaagattca atttaggcct aaatcttata atatcttcct ttaactttaa 258360aactacattt acttatgtgt gcgaggacat gtgtgtatgt gtgtgcatat aatccatgaa 258420cctctcccag acttgccact agtggtctat aaggattgtg tatggtttga atgtatacaa 258480ataacctttt aatacctcta tgcattgatt tgacccaaag aaactctaat gagtttcaat 258540tactcccgca tgctttagaa gcttcaagta ttagatgaaa tagaacaacc gtggggagtt 258600aaggttcgat tacattgcta ggtctttttt ttttttttgt ttccaacaaa acattatctg 258660tgttcattta gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga 258720gattaggagg ggggtctttg ggagaggatg aggtctgtaa aacaggattg gaattctcac 258780agaaggatca cggaaggatc cccatggaac gttcgccttg cttcaaatgt cagacatagc 258840tagacagcgc catctatgga tcagagggtg gccttcacca gatacttgat ggctggaacc 258900tgggtgtggt ataggagtat agaagagaga gcaagagaga gcgagagaag agagagagag 258960agagagagag agagagagag agagagagag agagagagag agagagtgag agagagagag 259020cgagagagcg agagagtgag agagagaaag cgagagagcg agagagtgag agagagaaag 259080cgagagagcg agagagagat gtttttatga gtctccatgc ctatagtact atggtatttt 259140gctagaacag tctgagtagg tttctgacaa cagctgccca gcttctgaaa gttccttgct 259200ttggctgaag aatgtccttt gaggaagttt tgtttctggg gttttgtttt gttttgtttt 259260gttttgtttt gttttgtttt gttttgaaaa aagagtctca tgtatcttgg gctggtctca 259320catttgaaat gtaccaaaga tcaccttgaa cttctgatcc tcctgactcc atgtcccaaa 259380cgatagaact gcagatgtgc actaccaccc ctggccctct gggcagtttt atttttaatt 259440ttcttattta tacatttatt tgtttgtgca ttgattgatt gattgattgt gtgtgtgtgt 259500gtgtgtgtgt gtgtgtgtat ggacacacac atgtgtgagt atgtgcatat gggtatgtat 259560gcatgtgtgc actccggcta tatgtatgta aaggtcagag gacaactttc gatggtgggt 259620tgggttccct ccctttacca tgcgggacct gaagattggg ctcaggtaat ccaacctgac 259680cgcaagcgcc tttacttact gagggaattg acctgtgact cctggtacag atgccaaaca 259740gcagcctctt acagcatggt gtggtggaag aggcctgcaa tcctaggatt aggaggatga 259800agcagtttgg gggccatact ggacttcaca gagagaatcc gtaaggaagg taggaaggaa 259860ggaataaagg gaggaaggga ggaagagaga ggagggatga agggaaggaa gaaggaaagg 259920gaagacaaga agaaaagaag gacaggggga agagggacaa aggcagcata gctctccacc 259980agtctggttt atggtgacgg cagctagagt cctctcctgc tttccctgat gtcttttttt 260040ctaagctgtg tcctccaact ttgcaggatt tttaaaaacc tagtccaatc agttaaattc 260100tggctcacat cttccagcac agaggtccat tccagcagat ggctcagcaa gtaaaggcgc 260160ttgcggtcag gttagattac ctgagcccaa tcttcaggtc ccgcatggta aagggaggga 260220acccaaccta cactcgaaag ttgtcctctg acctttccat ccatacaggg gtccaccaag 260280taagagcctt acccgacaca ccttctcatc ccttcttagg aagccctatg tgccttggcc 260340tttggaagcc atagtctttg ccctgcctgc acaagtcatg ctggcctgtc tgcaggaggc 260400ttagtcctta gaagattggt tgctcctcaa ggaaagagcc accccctttg tgctaagaca 260460ctgagtggac ctagtctccc atagcccagt ttatcccagg tcatctctag cagctaagat 260520actggacaaa ttgaaagtga catctgtctg ggcttaagtg accagtctca tctgtcctgt 260580tcagggaccc tctgacagct gagttcaagg ggaagagggg agtcctgata tgggtctaca 260640aacctctgta tttttttaag agagagagag agagagacag acagacagac agacagacag 260700acagacagac agagactaga acaggttagc cctgaccttg cctgtagcta agggtgactc 260760tggcatgctg atacccctcc tatcccctac ctcccacatt ctgagatttt ttgagccatc 260820atgcctggct tatccagtgc tggagactga actcagagtc ttgtgtatgc ttgtcaatca 260880ctcgactggc tgagccacat tcccagctct ggtattctat tttggatgtg tgatggtctg 260940gctccatagt ccagaacagc cttgaactcc tgaccctagg gtcagcccta aattctggga 261000ttacaaatgt atgtcaccat tcccagatat ttatgaaaat atctcccaaa gattttaagt 261060taatgatatg ttgacaaata cacgatacat acgtctattg ctacagcttg gatttgagct 261120gtccctcaaa ggcccatgtg gtaagatgtg accactagcc cctggcactg ttgggaggtg 261180gtggaatctt taggtgctgg gacccagtgg gagaaaagtc actggaggta tcatggtcct 261240tgaaagggat acaggtgcct ggtctctctg ctctccctcc ttgcttccct gtcagcacaa 261300ggtaaacaac ttccttcaaa cacatgttct cgccatgatg cccttcttga cataggtcca 261360aaagtaacag gctaactggc cttggaatct ctgagaccct tagagaaaaa taaactgttt 261420ttttttccac attaggttga tttctcgtgg gtatttgtaa tggtgacaga aaactgatga 261480acacagagac tgtatttgaa tatgataacc caaggcagtg tgtagttaag atagctggac 261540ctcagcaccc atcagtcaga ggcactgtta accatgctgg gcaatgaaca ggagaccctg 261600aaaaatggca ggaaggagat ttgaaatcca gatggcttca acaggccttt ggagagtaca 261660gagggaggag gggttgaaga agggaaaatg aagtccacga gaaggctaac cacccatctg 261720agaccgccat ggagaaaggc ccagtagcaa ggcctgtgag gcgcttctga caaacaatta 261780tccgcgttgg gtcattgtgt catcctttta acagactgtg acctcagtgg tgaagagggg 261840aagaaaaggc agttgggtcc ctaggggtgg acctgggatt tgaacacgtg atctacagac 261900tggaaacttt tctgttagct gccaagctgt gatggaagct gtgatggtga aatgttcata 261960gatgagcagg gatactcttg tacaaggagc aggcttgggt cctgtctcat catgctccaa 262020gcccagcatc aggctcttac cggagaaagg aggccggatg ctaaaggaac cgctcccagg 262080actcctgtga ggaagaaggg gcttccgttt ttattttata aatttctaaa acgttggaat 262140ttttctcctg gttttctttc tttctttatt tacatccaaa cgctgtccct tctcccagtc 262200ctccattgaa gtgttcctcc cccacatccc tcccctttgc ctctgagaag gttgccccct 262260cgcaggtatc ccccaaccct ggtgcatcaa gtctttgtag gattaagtgc atcctgtccc 262320actgaggcga gacaaggcag tccactgata catgtgtctc tgtgtgtgta tgtgggggtg 262380gtggtggtgg tggtgtttgg accagcccgt gcttgctctt tggttggtgg ctcagtcact 262440aggatcctca actctgcctc ctgagtactg ggaataaaaa tgtgagccgc caccaccctg 262500caaacattag aattttaaaa aagatttatt tttattttta ttttctgggc atgattgttt 262560tacctgcagg tatgtgtgtg tatcatgtgc atgcttggtg cccacagaga tgtgaagaag 262620gcatccaata ccctgaactg gagttacaga cagttgtgag gcactgcgtg agtgctggga 262680actgagcacg ggtcctctga aagagcagca agggttttaa cctttgagcc atccttccag 262740tcccagacat tggaattatt tttaaagatt tatttttgtt ttatatgtat gagtgttttg 262800cctgcaccac atgcatgccc agtgccttca gaggtcagaa gaggatgcca gatttccttg 262860aaatgaagtt acagacactt gtaagtcacc atgtgggtat tgagaacaaa gcccaggtct 262920tcagcaagtg ctggtaacca ctgagccatc cttccagccc aagatctggg atataaaaat 262980gaacttttat actacttttt tcttcccctt ccttccttcc ttcctttctt ccttccttca 263040taccatacag agatatataa gttccggact ctggaatacc tcttaaatat gtccctctgg 263100gtgagtgtat atacatgtgg gtgcgcgctt acatgcctcg catgctctct tgggctcttt 263160tcacagaatg ccctgtaacc agggtaaggt ggcactctct ttgagtaatg ataagaattg 263220gcccgctttc taatttccat atgcggctgt ccaaaacgaa aaaaggagtc gaccccttaa 263280gagctggaga ggccctcttg taaatacacg atcaaggtgg tccctcactg aataggttac 263340ttgggcgagt gggtcttcac atttcgtggt ctaaaaccac agcgctaacc tataaaatgc 263400gtgtccggca cgaaatacaa ttgcactccc gagaaaacac ccgcgggggg ttacgattga 263460atatctattg agagcacaac actctccccc tatttcggag aaactattgg gccctaccca 263520tgttttttat aaactctcaa atgggcccta cccaaacaac atatcgcggt tttttttgtt 263580aaatcacaac gcaaagaggg gtcgaaaata aatccctcgg cgggacctcg acacacaatc 263640tttcaccata tgagcgcgcc cacaacactg atcacatata tcgctgctcg cggagaaggt 263700accgacctct catctctttg gggtccgtcc acacaaaaga aaag 2637447366PRTHomo sapiens 7Met Ala Ser Met Ala Ala Val Leu Thr Trp Ala Leu Ala Leu Leu Ser1 5 10 15Ala Phe Ser Ala Thr Gln Ala Arg Lys Gly Phe Trp Asp Tyr Phe Ser 20 25 30Gln Thr Ser Gly Asp Lys Gly Arg Val Glu Gln Ile His Gln Gln Lys 35 40 45Met Ala Arg Glu Pro Ala Thr Leu Lys Asp Ser Leu Glu Gln Asp Leu 50 55 60Asn Asn Met Asn Lys Phe Leu Glu Lys Leu Arg Pro Leu Ser Gly Ser65 70 75 80Glu Ala Pro Arg Leu Pro Gln Asp Pro Val Gly Met Arg Arg Gln Leu 85 90 95Gln Glu Glu Leu Glu Glu Val Lys Ala Arg Leu Gln Pro Tyr Met Ala 100 105 110Glu Ala His Glu Leu Val Gly Trp Asn Leu Glu Gly Leu Arg Gln Gln 115 120 125Leu Lys Pro Tyr Thr Met Asp Leu Met Glu Gln Val Ala Leu Arg Val 130 135 140Gln Glu Leu Gln Glu Gln Leu Arg Val Val Gly Glu Asp Thr Lys Ala145 150 155 160Gln Leu Leu Gly Gly Val Asp Glu Ala Trp Ala Leu Leu Gln Gly Leu 165 170 175Gln Ser Arg Val Val His His Thr Gly Arg Phe Lys Glu Leu Phe His 180 185 190Pro Tyr Ala Glu Ser Leu Val Ser Gly Ile Gly Arg His Val Gln Glu 195 200 205Leu His Arg Ser Val Ala Pro His Ala Pro Ala Ser Pro Ala Arg Leu 210 215 220Ser Arg Cys Val Gln Val Leu Ser Arg Lys Leu Thr Leu Lys Ala Lys225 230 235 240Ala Leu His Ala Arg Ile Gln Gln Asn Leu Asp Gln Leu Arg Glu Glu 245 250 255Leu Ser Arg Ala Phe Ala Gly Thr Gly Thr Glu Glu Gly Ala Gly Pro 260 265 270Asp Pro Gln Met Leu Ser Glu Glu Val Arg Gln Arg Leu Gln Ala Phe 275 280 285Arg Gln Asp Thr Tyr Leu Gln Ile Ala Ala Phe Thr Arg Ala Ile Asp 290 295 300Gln Glu Thr Glu Glu Val Gln Gln Gln Leu Ala Pro Pro Pro Pro Gly305 310 315 320His Ser Ala Phe Ala Pro Glu Phe Gln Gln Thr Asp Ser Gly Lys Val 325 330 335Leu Ser Lys Leu Gln Ala Arg Leu Asp Asp Leu Trp Glu Asp Ile Thr 340 345 350His Ser Leu His Asp Gln Gly His Ser His Leu Gly Asp Pro 355 360 365821DNAMus musculus 8gaggatgtgg agctctaccg c 21922DNAMus musculus 9ctgtgtgcgc agagagtcta cg 221020DNAHomo sapiens 10cccgctgcag tccccagaat 201122DNAHomo sapiens 11cagggtcgag ggctcttgtc ct 221234DNAMus musculus 12tgactctaga tacccttggt cccatgttcc agat 341334DNAMus musculus 13cattgaattc gacaagagaa agacggggct caag 341439DNAMus musculus 14tatgactgcg gccgccacca

atcccacatc taagcatct 391521DNAMus musculus 15gctcggttct gggcacagag a 211623DNAMus musculus 16cttgaggatg ggcatcagct gta 231723DNAMus musculus 17gctcactaac agcgctcttg cct 231822DNAMus musculus 18acagttggag caaaggcgtg at 221922DNAMus musculus 19cttgctcgaa gctgcctttc ag 222025DNAHomo sapiens 20gattgattca agatgcattt aggac 252122DNAHomo sapiens 21ccccaggaac tggagcgaaa tt 222220DNAHomo sapiens and Mus musculus 22gcgcgtggtg ggrgaagaca 202320DNAHomo sapiens and Mus musculus 23tcgcgcagct ggtccaggtt 202424DNAHomo sapiens 24tgctcacctg ggctctggct cttc 242527DNAHomo sapiens 25ccagaagcct ttccgtgcct gggcggc 272630DNAHomo sapiens 26tggtgcacca cgaggctctg cagcagtccc 302727DNAHomo sapiens 27aggtggccct gcgagtgcag gagctgc 272831DNAHomo sapiens 28atgacgtggc agacgtaatg gcaagcatgg c 312927DNAHomo sapiens 29cgcgccgagg ataatggcaa gcatggc 273030DNAHomo sapiens 30gcctccctcc acctgtcttc tcagagcagt 303131DNAHomo sapiens 31atgacgtggc agacgaaaac gctgtggaga g 313227DNAHomo sapiens 32cgcgccgagg caaaacgctg tggagag 273323DNAHomo sapiens 33gcctttccgt gcctgggtgg cct 233429DNAHomo sapiens 34atgacgtggc agacgtggtg ggggaagac 293525DNAHomo sapiens 35cgcgccgagg atggtggggg aagac 253624DNAHomo sapiens 36aggagctgca ggagcagttg cgct 243721DNApPNT2T vector 37ctttttgtca agaccgacct g 213821DNApPNT2T vector 38aatatcacgg gtagccaacg c 213923DNAHomo sapiens 39atgacctgtg ggaagacatc act 234023DNAHomo sapiens 40agccagaagt gactagagcc aaa 234123DNAHomo sapiens 41agtccccaga atcaaaggat gat 234222DNAHomo sapiens 42atcgtgtagg gcttcagttg ct 224324DNAHomo sapiens 43cctgtcttct cagagcaggt aatg 244423DNAHomo sapiens 44agccatcttc tgctgatgga tct 234523DNAHomo sapiens 45aagacaccct agcctccttg act 234621DNAHomo sapiens 46acagaggttg aggcagcaga g 214723DNAHomo sapiens 47gtagtgaaaa tcaggggcct tct 234823DNAHomo sapiens 48atgcataaac ccaaagggaa aat 23

Patent applications by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Patent applications in class 25 or more peptide repeating units in known peptide chain structure

Patent applications in all subclasses 25 or more peptide repeating units in known peptide chain structure

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Patent application title: Novel apolipoprotein gene involved in lipid metabolism

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