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The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis

Article Abstract:

The RBM and DAZ/SPGY genes are protein encoding genes located on the Y chromosome, containing RNA-binding motifs. Neither gene family is thought to be essential for spermatogenesis in human males. The genes are suited to the knockout approach, but are represented only by the autosomal gene DAZLA in mammals other than Old World primates and apes. DAZLA protein is shown to be cytoplasmic in male and female germ cells, and disruption results in loss of germ cells and the absence of gamete production.

Spermatogenesis

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Germ-line transmission and expression of a human-derived yeast artificial chromosome

Article Abstract:

Germ-line transmission and the introduction of huge human DNA fragments into the embryonic system of mice is demonstrated. These DNA molecules are cloned into yeast artificial chromosomes for easy manipulation and greater stability. The process did not impair development, germ-line transmission or gene expression. These results show that the technique could be useful in genetic complementation studies of recessive genetic disorders and in creating new mouse strains.

Yeast fungi, Microbial biotechnology, Yeasts (Fungi), Genetically modified mice

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