Structural and mechanistic insights into the interaction between Rho and mammalian Dia

Article Abstract:

The crystal structure of RhoC in complex with the regulatory N terminus of mammalian Diaphanous 1 (mDia1) containing the GBD/FH3 region, an all-helical structure with armadillo repeats is presented. Although bindings of Rho and Diaphanous autoregulatory domain (DAD) on the N-terminal fragment of mDia1 are mutually exclusive, their binding sites are only partially overlapping.

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A structural basis for allosteric control of DNA recombination by lambda integrase

Article Abstract:

A report on the crystal structures of lambda-int in higher-order complexes with substrates and regulatory DNAs representing different intermediates along the reaction pathway is presented. The structures demonstrate how the simultaneous binding of two separate domain of lambda-int to DNA facilitates synapsis, and can specify the order of DNA strand cleavage and exchange.

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Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain

Article Abstract:

The conserved formin homology 2 (FH2) domain nucleates actin filaments and remains bound to the barbed end of the growing filament. Each of the two structural units in the FH2 dimer binds two actins in an orientation similar to that in an actin filament hinting that this structure could function as a filament nucleus.

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