Production of the antimalarial drug precursor artemisinic acid in engineered yeast

Article Abstract:

A study of the engineering of Saccharomyces cerevisiae to produce high titres of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4, 11-diene to artemisinic acid is presented. The synthesized artemisinic acid is transported out and retained on the outside of the engineered yeast, meaning that a simple and inexpensive purification process can be used to obtain the desired product.

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Amplification of histone genes by circular chromosome formation in Saccharomyces cerevisiae

Article Abstract:

A study shows that a different mechanism of dosage compensation, at the level of gene copy number, can occur when HTA1-HTB1(the histones H2A and H2B are encoded by two gene pairs in Saccharomyces cerevisiae) is deleted and HTA2-HTB2 amplifies via creation of a new, small, circular chromosome. The results have demonstrated another mechanism by which histone gene dosage is controlled to maintain genomic integrity.

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Golgi maturation visualized in living yeast

Article Abstract:

A direct test of the two models cisternal maturation model and stable compartment model is carried out using three-dimension time-lapse fluorescence microscopy of the yeast Saccharomyces cerevisiae. The results of the test reveal that individual cisternae mature, and do so at a consistent rate.

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