Transformation of chlorinated benzenes and toluenes by Ralstonia sp. strain PS12 tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene dihydrodiol dehydrogenase) gene products
Article Abstract:
Research has been conducted on the tecB gene located downstream of tecA and encoding tetrachlorobenzene dioxygenase in Ralstonia sp. strain PS12. The results of the tecB gene cloning into the Escherichia coli DH5alpha together with the tecA gene are described.
author: Pollmann, Katrin, Beil, Stefan, Pieper, Dietmar, H.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Genetic aspects, Cloning, Benzene, Microbiological research
Rational engineering of the regioselectivity of TecA tetrachlorobenzene dioxygenase for the transformation of chlorinated toluenes
Article Abstract:
Research has been conducted on Ralstonia sp. PS12 tetrachlorobenzene dioxygenase. The authors have investigated this dioxygenase which is involved in chlorinated toluene biodegradation, and report the results.
author: Pieper, Dietmar, Pollmann, Katrin, Wray, Victor, Hecht, Hans-Jurgen
Publisher: Society for General Microbiology
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2003
Science & research, Petrochemical Manufacturing, Toluene, Biodegradation, Oxidases, Microbiology
cis-Chlorobenzene dihydrodiol dehydrogenase (TcbB) from Pseudomonas sp. strain P51, expressed in Escherichia coli DH5alpha (pTCB149), catalyzes enantioselective dehydrogenase reactions
Article Abstract:
Pseudomonas sp. strain P51 cis-chlorobenzene dihydrodiol dehydrogenase (CDD)(TcbB) expressed in Escherichia coli DH5alpha (pTCB149) has been found to catalyze enantioselective dehydrogenase reactions. The study was carried out with the object of investigating the potential of CDD for enantiomeric resolution of chiral dihydrodiols. CDD preferentially oxidized the para-halogenated cis-toluene dihydrodiol enantiomers preferentially formed by chlorobenzene dioxygenase (CDO).
author: Meer, Jan Roelof van der, Raschke, Henning, Kohler, Hans-Peter E., Fleishmann, Thomas
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
Switzerland, Enzymes, Pseudomonas, Cytochemistry, Enantiomers
subjects list: Statistical Data Included, Analysis, Germany, Physiological aspects, Escherichia coli, Dehydrogenases, Oxidoreductases, Toluene, Chlorination, Research
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