Article Abstract:
Analysis of the potential of the flagellar domain of Campylobacter jejuni to produce genetic diversity reveals the occurrence of recombination subsequent to exogenous DNA uptake and within the genome. A bacterial clone with an antibiotic-resistance gene in flaA exhibits a spontaneous recombination in the C. jejuni flagellar filament subunit that the flaA and flaB tandem genes code for. Repositioning of the antibiotic-resistance gene into flaB in the isolated recombinant suggests the exchangeability of genetic information between the C. jejuni flagellin genes. Addition of purified chromosomal DNA to a recipient strain yields double-resistant transformants, when co-cultured or under natural competence-inducing conditions.
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Article Abstract:
Gene cloning technique using the lambda-10E6 of the Kohara collection as the source of DNA was employed to yield the ldhA gene that encode the fermentative lactate dehydrogenase of Escherichia coli. Subsequent sequencing of the cloned gene revealed that it is homologous to genes encoding other D-lactate-specific dehydrogenases (d-LDH). The gene was also found unrelated to L-lactate-specific enzymes. ldhA can escape negative regulation as evidenced by its overexpression in cells when it is present in high copy number.
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Article Abstract:
The flaA and flaB flagellin genes of Campylobacter are not entirely identical. One segment of the flagellin sequence evolves while the other segment is maintained by conversion or recombination.
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