Engineering desiccation tolerance in Escherichia coli

Article Abstract:

Sucrose maintains membrane fluidity during cell dehydration. This could explain how the disaccharide can protect cells from dehydration. E. coli that expressed the sucrose-6-phosphate synthase gene had a 10,000-fold increase in tolerance to desiccation compared to the wild-type bacteruim.

author: Potts, Malcolm, Crowe, John H., Billi, Daniela, Wright, Deborah J., Helm, Richard F., Prickett, Todd
Escherichia coli, Dehydration (Physiology), Sucrose

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Unusual water flux in the extracellular polysaccharide of the cyanobacterium Nostoc commune

Article Abstract:

The authors have investigated desiccated Nostoc commune. They have discovered that bacterium's water uptake speed depends on desiccation duration, and that the evaporative water loss is affected by the transcription and translation inhibitors.

author: Potts, Malcolm, Helm, Richard F., Shaw, Eric, Hill, Donna R., Britain, Nicole, Wright, Debbie J., Tauber, Uwe, Marand, Herve
Environmental aspects, Cells (Biology), Bacteria, Microbiology, Cells, Microbial populations, Nitrogen fixation, Microbial ecology

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Transcriptional response of Saccharomyces cerevisiae to desiccation and rehydration

Article Abstract:

The transcriptional response of Saccharomyces cerevisiae strain BY4743 to desiccation and rehydration under glucose-limiting conditions is analyzed. The application of a redescription-mining algorithm has indicated that sulfur metabolism is very important for cell survival during desiccation and rehydration.

author: Potts, Malcolm, Helm, Richard F., Singh, Jatinder, Kumar, Deept, Ramakrishnan, Naren, Singhal, Vibha, Jervis, Jody, Garst, James F., Slaughter, Stephen M., DeSantis, Andrea M.
Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Brewers' Yeast, Microbial metabolism, Genetic transcription, Transcription (Genetics), Brewer's yeast, Saccharomyces cerevisiae

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subjects list: Research, Physiological aspects, United States, Genetic aspects
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