Article Abstract:
In the presence of an external source of energy Thermotoga neapolitana accumulates the nonmetabolizable glucose analog, 2-deoxy-D-glucose against a concentration gradient. This indicates the presence of an active transport system for glucose in the hypertheromophilic anaerobic marine bacterium. 2-deoxy-D-glucose is accumulated in an intracellular free sugar pool that is exchangeable for extracellular D-glucose. The transport depends on the presence of Na+ ion and is inhibited by arsenate and gramicidin D. There is absence of any sugar phosphotransferase activity in T. neapolitana cells.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
The suitability of Thermotoga neapolitana for basic molecular biological studies of extreme thermophiles is discussed. Its growth medium components and cultivation conditions were optimized by a formulated defined marine salts medium. Mean plating efficiencies were calculated under aerobic and anaerobic conditions. Its ability to grow on both solid and liquid media with minimal anoxic precautions and its variability despite being a strict anaerobe determined the importance of this evolutionary bacterium.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is investigated. Results indicate that the bacterium can regulate beta-galactosidase synthesis and exhibit diauxic growth in media containing glucose and lactose. Moreover, it was shown that different sugars affect beta-galactosidase expression in different ways and that cAMP does not play a role in beta-galactosidase regulation.
User Contributions:
Comment about this article or add new information about this topic: