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Polysaccharide lyases from gellan-producing Sphingomonas spp

Article Abstract:

Several Sphingomonas strains capable of synthesizing the bacterial exopolysaccharide gellan and related polymers were found to have constitutive gellanase activity. The degradation of deacylated gellan was due to extracellular, eliminase-type enzymes or lyases which cleave the sequence ...beta-D-glucosyl 1,4-beta-D-glucuronosyl... in the tetrasaccharide repeat unit of the substrate polysaccharides. The enzymes had very little activity against the natural, acylated gellan and rhamsan polysaccharides from Sphingomonas strains.

Enzymes

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Identification of two glycosylated components of Mycoplasma penetrans: a surface-exposed capsular polysaccharide and a glycolipid fraction

Article Abstract:

Transmission electron microscopy revealed the presence of a 11- to 30-nm capsular material surrounding Mycoplasma penetrans. Staining studies suggest that this capsular material is composed of polysaccharides and anchored to the plasma membrane by polypeptides. Electrophoretic analysis revealed the presence of high- and low-molecular mass glycosylated materials in the capsule with different heat and enzyme sensitivities.

Composition, Glycolipids, Bacterial cell walls, Mycoplasma

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A novel gene, algK, from the alginate biosynthetic cluster of Pseudomonas aeruginosa

Article Abstract:

The cloning and sequencing of the new gene called algK which is involved in the biosynthesis of alginate from Pseudomonas aeruginosa was identified. This gene which lies between alg44 and algE genes of the operon encodes a poorly expressed preprotein of approximately 53 kDa which is processed to form a mature polypeptide. AlgK is found to be periplasmic in location and is most likely anchored to the inner membrane.

Analysis, Pseudomonas aeruginosa, Cystic fibrosis

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