Article Abstract:
The physiology of the deep-sea hyperthermophilic, anaerobic vent archaeon Pyrococcus abyssi from the Fiji Basin at a depth of 2,000 m was investigated under various conditions. Incubation under in situ pressure (20 MPa) and at 40 MPa increased the maximal and minimal growth temperatures by 4 degrees C. In situ pressure improved survival at a lethal high temperature (106 to 112 degrees C) relative to that at low pressure (0.3 MPa). The whole-cell protein profile did not change in cultures grown under low or high pressure at optimal and minimal growth temperatures.
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Article Abstract:
Experimental studies using hyperthermophilic sulfur-metabolizing archaeon Pyrococcus abyssi GE5, isolated from deep-sea hydrothermal vents, reveal that growth of these species requires a minimum of nine amino acids and vitamins as the sole carbon and energy sources. Cell densities and the generation time in the minimal growth medium are measured.
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Article Abstract:
Researchers describe an alkaline phosphatase isolated from the hyperthermophilic archaeon Pyrococcus abyssi. The enzyme has a similar active site and many of the same structural elements as the Escherichia coli alkaline phosphatase.
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