Phosphate stress in cultures and field populations of the dinoflagellate Prorocentrum minimum detected by a single-cell alkaline phosphatase assay

Article Abstract:

Enzyme-labeled fluorescence (ELF)-97 labeling in axenic cultures of a common dinoflagellate, Prorocentrum minimum was characterized to elucidate on ELF-97 dynamics when phosphate nutrition varies. After laboratory cultures were characterized, ELF-97 was employed to assay field populations of P. minimum in Narragansett Bay. The level of cell labeling was reduced by three days of incubation with added inorganic phosphate. Findings showed that ELF-97 is an excellent technique for monitoring phytoplankton phosphate stress in the environment when the data are supported by appropriate laboratory studies.

Author: Palenik, Brian, Dyhrman, Sonya T.
Research, Usage, Dinoflagellates, Alkaline phosphatase

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Long serial analysis of gene expression for gene discovery and transcriptome profiling in the widespread marine coccolithophore Emiliania huxleyi

Article Abstract:

A study was conducted on the role of the abundant and widespread coccolithophore Emiliania huxleyi in mediating CO(sub 2) exchange between the ocean and the atmosphere through its impact on marine photosynthesis and calcification. It was observed that the identification of E. huxleyi genes responsive to nitrogen (N) or phosphorus (P) starvation is done by the use of long serial analysis of gene expression.

Author: Dyhrman, Sonya T., Haley, Sheean T., Birkeland, Shanda R., Wurch, Louie L., Cipriano, Michael J., McArthur, Andrew G.
Analysis, Polymerase chain reaction, Gene expression, Photosynthesis research, Coccoliths

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Phosphorus scavenging in the unicellular marine diazotroph Crocosphaera watsonii

Article Abstract:

Genomic and physiological approaches were used to examine the phosphorus scavenging mechanisms in strains of Crocosphaera watsonii from both the Atlantic and Pacific Oceans. The data suggests that C. watsonii has a robust capacity for scavenging phosphorus in oligotrophic systems, although this capacity differs from that of other marine cyanobacterial genera.

Author: Dyhrman, Sonya T., Haley, Sheean T.
Cyanobacteria, Marine bacteria

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Subjects list: Physiological aspects, Genetic aspects
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