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Macrophage-derived metalloelastase is responsible for the generation of angiostatin in Lewis lung carcinoma

Article Abstract:

Molecular biological techniques were used to investigate the mechanism involved in the in vivo production of angiostatin, a product of enzymatic action of elastase on plasminogen which inhibits growth and metastasis in Lewis lung carcinoma (3LL). The results of the experiment which was done on syngeneic mice revealed that tumors became infiltrated by macrophages and started expressing high levels of steady-state mRNA for metalloelastase (MME) post-implantation of 3LL variant cells into the subcutis of the mice. The data also provided evidence that angiostatin is produced by tumor-infiltrating macrophages the MME expression of which is activated by tumor cell-derived granulocyte-macrophage colony-stimulating factor.

Analysis, Usage, Polymerase chain reaction, RNA, Metalloenzymes, Lung cancer, Chromatography, Enzymatic analysis, Immunohistochemistry, Western immunoblotting, Western blot

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Macrophages: Obligate partners for tumor cell migration, invasion and metastasis

Article Abstract:

Macrophages within the tumor microenvironment facilitate angiogenesis and extra cellular matrix breakdown and remodeling and promote tumor cell motility. It is revealed that direct communication between macrophages and tumor cells leads to invasion and egress of tumor cells into the blood vessels (intravasation), thus macrophages are an important drug target for cancer therapy.

Tumors, Tumours

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VEGF-induced adult neovascularization: Recruitment, retention, and role of accessory cells

Article Abstract:

The endothelial growth factor VEGF is sufficient for organ homing of circulating mononuclear myeloid cells and is required for their perivascular positioning and retention. The data suggests a model for VEGF-programmed adult neovascularization highlighting the essential paracrine role of recruited myeloid cells and a role for SDF1 in their perivascular retention.

Endothelial growth factors

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