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Localization of denitrification genes on the chromosomal map of Pseudomonas aeruginosa

Article Abstract:

Cleavage of chromosomal DNA from Pseudomonas aeruginosa PAO by SpeI and DpnI were employed to determine the map location of certain primary denitrification genes. These are narGH, nirS, nirE, norCB, nosZ and nosA. Genes related to anaerobic or microaerophilic metabolism were also examined. These are napA, ccoN, hemN, an fnr-like regulatory gene, and azu and fdxA. A homologue of nosA encoding a putative portion of the Cu-processing mechanism for nitrous-oxide reductase was identified. There is an indication of a linkage of anr with hemN and ccoN, and the presence of a napA gene in both P. aeruginosa and P. stutzeri.

Chromosomes, Denitrification

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Protein C (OprC) of the outer membrane of Pseudomonas aeruginosa is a copper-regulated channel protein

Article Abstract:

Cloning and sequencing of the protein C (OprC), one of three porins of the outer membrane of Pseudomonas aeruginosa, showed that it functions as a channel-forming and copper-binding protein. Its nucleotide sequence revealed that it encoded a polypeptide consisting of 723 amino acid residues for the precursor and 668 for mature polypeptides. Its expression seemed to be controlled by exogenous Cu2 and liberalized by anaerobiosis in the presence of nitrate, indicating that the protein may play a role in copper utilization.

Analysis, Protein C

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Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa

Article Abstract:

The existence of a single glycerol transport consisting of a glycerol diffusion facilitator (GlpF) and glycerol kinase (Glpk) in Pseudomonas aeruginosa was suggested to be similar to the corresponding proteins of Escherichia coli GlpF. Identification of related sequences in Gram-negative bacteria revealed conservation of the molecular architecture of bacterial glycerol uptake systems.

Biological transport, Glycerol, Glycerol kinase

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