Article Abstract:
A physical chromosome map has been made of the European stone fruit yellows (ESFY) phytoplasma strain GSFY1 as a first step toward a detailed genetic characterization of the ESFY phytoplasma. ESFY phytoplasma is an important prokaryotic pathogen that infects most kinds of stone fruits in Europe. The map, made using PFGE-purified genomic DNA from diseased tobacco and tomato plants, was not identical to that of the closely related apple proliferation phytoplasma, but was similar.
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Article Abstract:
The Mesorhizobium sp. strain R7A bio operon on its acquired symbiosis island of has been found to include a novel gene that is part of the pimeloyl-coA synthesis. The reason the vitamin biosynthetic loci are on an acquired genetic element not in nonsymbiotic mesorhizobia is not yet known. The symbiosis island is a 500-kb chromosomal genetic elements that when transferred converts nonsymbiotic mesorhizobia to symbionts that can nodulate and fix nitrogen with Lotus corniculatus. A 13.2-kb region of the island that changed the nonsymbionts to nicotinate and biotin prototrophy has been characterized.
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Article Abstract:
A study of the immunodominant membrane protein (IMP) of the apple proliferation phytoplasma (AP) from AP-infected periwinkle plants resulted in the isolation of the gene encoding the P-318B IMP. The isolated gene was expressed in Escherichia coli and the resulting protein used in the immunization of rabbits. The study resulted in the development of a novel approach to generating immunogens for the production of specific antibodies for the characterization and detection of non-culturable phytoplasmas.
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