Immunization with recombinant class 1 outer-membrane protein from Neisseria meningitidis: influence of liposomes and adjuvants on antibody avidity, recognition of native protein and the induction of a bactericidal immune response against meningococci

Article Abstract:

Research was conducted to examine the high-level expression of the class 1 protein in Escherichia coli in the absence of a large N-terminal leader protein and in a form that can be readily purified. The recombinant protein was utilized in experiments with mice to investigate the impact of vaccine formulations, compatible with human immunization, on the avidity and specificity of the antibodies induced. Results indicated that antibodies to the class 1 and Opc proteins had the most substantial roles during bactericidal activity against the vaccine strain.

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Variation within serovars of Neisseria gonorrhoeae detected by structural analysis of outer-membrane protein PIB and by pulsed-field gel electrophoresis

Article Abstract:

The use of molecular methods based on PIB sequence analysis and restriction endonuclease digestion of gonococcal DNA were investigated. The sequence of por genes encoding protein PIB were obtained from a series of strains to investigate variations within a serovar. Sequence differences between gonococcal strains is important for epidemiological studies of gonococcal infection but also in providing information on sexual mixing patterns used in mathematical models of HIV-transmission dynamics.

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Effect of sialylation of lipopolysaccharide of Neisseria gonorrhoeae on recognition and complement-mediated killing by monoclonal antibodies directed against different outer-membrane antigens

Article Abstract:

The effectiveness of monoclonal antibodies against the sialylation of lipopolysaccharide of Neisseria gonorrhoeae was measured by their ability to bind to the bacteria grown in CMP-N-acetyl neuraminic acid. The bacteria also demonstrate resistance to normal human serum, which inhibits the ability to develop an effective vaccine. Monoclonal antibodies are able to recognize surface-exposed epitopes such as PI on sialylated gonorrhea bacteria, but may not be effective in promoting its killing.

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