Article Abstract:
The three main numerically abundant culturable bacterial isolates in marine communities have been identified as belonging to the alpha-subclass and gamma-subclass of Proteobacteria. Dot blot and colony hybridization were used to obtain the isolates from lignin enriched culture. Isolate-specific 16S rRNA oligonucleotide probes showed that the isolates contribute 2-32% of the total DNA content. The partial sequences of 14 isolates; which included Proteobacteria, Cytophaga-Flavobacterium, and gram-positive groups; revealed substantial phylogenetic variety and unusual sequences.
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Article Abstract:
A research was conducted to study the application of in situ reverse transcription (ISRT) for detecting and quantifying lignin-degrading bacterial species in lignin enrichment communities and Pseudomonas putida F1 which expressed a toluene degradation gene when they were added to seawater exposed to toluene. The non-labeled primer was hybridized to the RNA target site while a general DNA stain was utilized to visualize all sample cells. Results showed that the ISRT protocol was sensitive enough to detect 16s rRNA and mRNA in complex microbial communities.
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Article Abstract:
Oligonucleotide probes directed against the ribosomal DNA in the 16S subunit were designed to survey bacterioplankton in marine and estuarine environments in the southeastern coast of the US. Results reveal the predominance of a cluster of marine bacteria in the alpha-3-subclass of Class Proteobacteria. The other applications of the generated ribosomal DNA probes are discussed.
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