Identification of type A, B, E, and F botulinum neurotoxin genes and of botulinum neurotoxigenic clostridia by denaturing high-performance liquid chromatography

Article Abstract:

Denaturing high-performance liquid chromatography (DHPLC) is a new method for speedy viewing of nucleotide polymorphisms in PCR products, and this method is utilized to check the type A, B, E, and F botulinum neurotoxin genes. Distinct reports were procured for each genus and species, indicating that this method would be a dependable alternative to the progression of speedy detection of food borne pathogens.

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Biosensor detection of botulinum toxoid A and staphylococcal enterotoxin B in food

Article Abstract:

Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.

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Characterization of neutralizing antibodies and identification of neutralizing epitope mimics on the Clostridium botulinum neurotoxin type A

Article Abstract:

Researchers used the phage display method to identify the B-cell epitopes of two monoclonal antibodies against Clostridium botulinum neurotoxin type A. This information could be useful in designing a vaccine against botulism.

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