Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool

Article Abstract:

Researchers used a small-subunit rRNA-based PCR restriction fragment length polymorphism to identify 12 genotypes of Cryptosporidium in 29 water samples from a stream. This method can be used instead of an immunofluorescent assay.

author: Lal, Altaf A., Xiao, Lihua, Limor, Josef, Alderisio, Kerri, Royer, Michael

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Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater

Article Abstract:

Researchers can use small-subunit rRNA-based PCR-restriction fragment length polymorphism to analyze water samples for the presence of Cryptosporidium. This technique can differentiate between Cryptosporidium strains that affect humans and those that do not.

author: Xiao, Lihua, Limor, Josef, Lal, Altaf, Singh, Ajaib, Graczyk, Thaddeus K., Gradus, Steve

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Genetic diversity of Cryptosporidium spp. in captive reptiles

Article Abstract:

The genetic diversity of cryptosporidium in reptiles was analyzed using PCR-restriction fragment length polymorphism and sequence analysis of a small subunit rRNA gene. In the study samples of snakes, lizards and tortoises were analyzed and results suggest extensive genetic diversity.

author: Sulaiman, Irshad M., Lal, Altaf A., Xiao, Lihua, Limor, Josef, Graczyk, Thaddeus K., Arrowood, Michael J., Zhou, Ling, Ryan, Una M., Li, Lixia, Kombert, Mark, Koudela, Bretislav, Junge, Randy, Modry, David
Science & research, Research, Case studies, Biological diversity, Biodiversity, Reptiles

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subjects list: Usage, Measurement, Water, Aquatic microbiology, Polymerase chain reaction, Coccidia, Cryptosporidium
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