Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation

Article Abstract:

Bacteria closely related to Rhodocyclus and Propionibacter are important polyphosphate-accumulating organisms in wastewater treatment systems that use enhanced biological phosphorus removal. This was the conclusion of researchers who used fluorescence in situ hybridization to determine the microbial content of sludge.

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Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors

Article Abstract:

The dominant bacterial species in sludge samples taken from two sequencing batch reactors (SBRs) belonged to the beta proteobacterial subclass. Both the samples also contained a large number of bacteria from the alpha proteobacterial subclass, the planctomycete group and the Flexibacter-Cytophaga-Bacteroides group, but Acinetobacter species were present in very little number. However, in the phosphate-removing sludge the Rhodocyclus group in the beta subclass was present in greater number than in the non-phosphate-removing sludge.

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Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems

Article Abstract:

The analysis of microbial populations during the operation of a laboratory-scale reactor with several phosphorus removal performances was conducted to examine the bacteria that are important to phosphorus. Fluorescence in situ hybridization with oligonucleotides probes complementary to regions of the 16S and 23S rRNAs was used to investigate the bacterial population structure. Results implicate bacteria of the beta-2 subclass of the class Proteobacteria and actinobacteria in high-performance phosphorus removal.

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