Identification of some ectomycorrhizal basidiomycetes by PCR amplification of their gpd (glyceraldehyde-3-phosphate dehydorgenase) genes

Article Abstract:

Polymerase chain reaction (PCR) amplification of glyceraldehyde-3-phosphate dehydrogenase (gpd) is useful for the detection of ectomycorrhizal basidomycetes during symbiotic interaction. The gpd genes can also be used to assess samples of DNA from the mycorrhizal roots. Fungal gpd genes are present in single or double copies in the genome. The gpd gene expresion signals may be helpful in the introduction of reporter genes.

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Quantitation of viral DNA by real-time PCR applying duplex amplification, internal standardization, and two-color fluorescence detection

Article Abstract:

Researchers tested a real-time PCR method that uses an internal standard on human parvovirus B19 DNA and found that it has high precision and reduces the risk of a false-negative result. Internal standards are created by coextracting the standard together with the nucleic acids and simultaneously amplifying both. Thus, there is no need to generate an external standardization curve.

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Gene cassette PCR: sequence-independent recovery of entire genes from environmental DNA

Article Abstract:

Researchers have developed a technique for detecting bacterial genes in environmental samples even when a genetic sequence is lacking. It is a PCR assay based on the gene cassettes associated with integrons. Most of the gene cassettes contained open reading frames that were not homologous to any known sequence.

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