Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA

Article Abstract:

A complementary PCR-restriction fragment length polymorphism (RFLP) method is useful for the identification of new cry genes in Bacillus thuringiensis strains. The method uses two universal oligonucleotide primer sets containing cry gene conserved sequences that are probably present in all cry genes. The presence of a new RFLP pattern indicates a possible cry gene. The method is also useful for the identification of the copy number and organization of the cry genes on the plasmid.

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Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA

Article Abstract:

Researchers were able to detect brown-rot and white-rot basidiomycetes in wood using the primer pair ITS1-F AND ITS4 to amplify fungal DNA using the polymerase chain reaction. The fungi could be detected before there was measurable loss of wood. Restriction fragment length polymorphisms of the internal transcribed spacer region were used to identify the fungi down to the species level.

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Metal accumulation without enhanced oxalate secretion in wood degraded by brown rot fungi

Article Abstract:

Brown rot fungi were incubated in agar and agar-wood microcosms containing metallic or hydroxide forms of AI, Cu, and Fe. Metal dissociation was associated with elevated oxalate concentrations in agar, but metals translocated into wood did not affect oxalate accumulation, crystal production, or decay rate, thus showing a substrate-dependent oxalate dynamic.

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