Article Abstract:
A microsatellite-based polymerase chain reaction (PCR) DNA fingerprint assay for the identification of Epichloe endotypes in culture and in planta has been developed. Epichloe endotypes are a group of filamentous fungi with sexual and asexual species. Identifying the endotype strains was carried out by constructing M13mp19 partial genomic libraries for size-fractionated genomic DNA. The libraries were then screened with a mixture of DIG-labeled dinucleotide and trinucleotide probes. Nine unique microsatellite loci were identified after positive clones have been sequenced.
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Article Abstract:
Cloning and sequencing of two DNA polymerase chain reaction products having different sizes reveal that they possess the same DNA sequence barring a stretch of tandem repeats (microsatellite) of AAG.TTC. The microsatellites differ in size. This method of identifying microsatellite loci in fungal genome can be used to determine the genotypic and ecological diversity of fungi. The frequency of microsatellite loci in fungal genome is an indication of the potential of the genetic marker approach to fungal diversity studies.
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Article Abstract:
Cloning and analysis of a nonribosomal peptide synthetase (NRPS) gene from Neotyphodium lolii has disclosed a putative gene cluster for the biosynthesis of ergovaline containing a single-module NRPS gene, IpsB and other genes. A genetic foundation is provided for describing biochemical steps in the ergovaline pathways, the ecological role of individual ergot alkaloid compounds and the regulation of their synthesis in planta.
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