Identification of an NADH-cytochrome b5 reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae

Article Abstract:

The isolation and characterization of the NADH-cytochrome b5 reductases (CbR) gene from Mortierella alpina 1S-4 and the purification of the CbR expressed in Aspergillus oryzae are described. It was found that the purified CbR used NADH but not NADPH as an electron donor. When NADH was utilized as the electron donor, DCPIP was observed to be reduced by the purified CbR. Results provide support for the hypothesis that the cDNA cloned is definitely a CbR gene.

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Isolation and use of a homologous histone H4 promoter and a ribosomal DNA region in a transformation vector for the oil-producing fungus Mortierella alpina

Article Abstract:

Researchers isolated the H3 and H4 histone genes from Mortierella alpina and used the H4 promoter in a transformation vector to induce the expression of a gene for hygromycin B resistance. This fungus is of interest to indusrial microbiologists because it produces long-chain polyunsaturated fatty acids.

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Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase

Article Abstract:

Laccase from the white-rot fungus Trametes versicolor can be expressed in Saccharomyces cerevisiae, which will make the yeast resistant to phenolic compounds produced during the fermentation of lignocellulose. Lignocellulose can be fermented to ethanol, which can be used as a fuel.

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