Article Abstract:
The study identifies a centrin/hsp complex in CSF-arrested Xenopus oocytes which dissociates with treatment activating the oocyte for embryological development. It also proposes a model for centrin sequestration prior to centrosome assembly, which is in keeping with hsp and chaperon functions in other systems. The probability of the usefulness of complex formation with the molecular chaperones to enable maintain oocyte protein stores in a quiescent state before their recruitment into particular fates in the developing embryo is also discussed.
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Article Abstract:
Research indicates different roles for cyclin A-cdc2 kinase and cyclin B-cdc2 kinase during oocyte maturation. Cyclin A cDNA clones were isolated from a library of goldfish oocyte cDNA. Monoclonal antibody from goldfish cyclin A recognized a 47-kDa protein that was not present after the egg was activated. Cyclin A was found in immature oocytes, unlike goldfish cyclin B, and did not show significant changes in protein level during maturation. In mature oocytes, goldfish cyclin A was associated with cdc2 kinase but not with cdk2.
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Article Abstract:
A study of maturation-promoting factor (MPF) activation during the maturation of goldfish oocytes reveals that phosphorylation and dephosphorylation of the catalytic subunit of MPF, cdc2, by Thr161 and Tyr15, respectively, affect MPF activation. The resultant structure of activated MPF, in which cdc2 is bound to a regulatory subunit cyclin B and is phosphorylated on Thr161, is independent of the mechanism of activation.
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