Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor

Article Abstract:

Research was conducted to examine the nitrifying community of a chemolithoautotrophic fluidized bed reactor using microsensors and rRNA-based molecular techniques. The reactor was operated with low ammonia concentrations instead of the high ammonia concentrations it was originally intended for. In situ activity measurements suggest that both ammonia and nitrite oxidizers were active within a 125 micrometer zone at the aggregate surface. Results agree with observations that in natural systems low in ammonium, Nitrosospira spp. can be detected rather than Nitrosomonas spp.

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Identification of bacteria potentially responsible for oxic and anoxic sulfide oxidation in biofilters of a recirculating mariculture system

Article Abstract:

The bacteria presumably involved in oxygen- or nitrate-dependent sulfide oxidation in the biofilters of a recirculating marine aquaculture system were identified, using a new application of reverse transcriptional-PCR denaturing gradient gel electrophoresis (DGGE) analysis termed as differential-transcriptional (DT)-DGGE. For the organisms the psychology of their closest cultured relatives matched their DT-DGGE-inferred functions.

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Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: quantification by in situ hybridization and the use of microsensors

Article Abstract:

Microsensors and in situ hybridization can be used to document microbial populations in various parts of a fixed-film treatment plant. At these plants, immobilized microorganisms are used to purify wastewater.

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