Article Abstract:
The Msn2p and Msn4p transcription factors of Saccharomyces cerevisiae are hyperphosphorylated by different stress conditions and are differentially regulated. cAMP reverses the phosphorylation, and the results suggest that PKA and stress-activated kinases control these transcription factors by antagonistic phosphorylation.
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Article Abstract:
The effect of cAMP on protein synthesis and, thus, the role of the cAMP pathway in regulating gene expression in Saccharomyces cerevisiae, is determined using two-dimensional gel electrophoresis. Results indicate that cAMP plays a minor role on the protein pattern of cells growing exponentially on glucose, although it interfered with changes in gene expression in yeast cultures. A model that gives the cAMP pathway a role in maintaining gene expression is proposed. Such a model would require a decrease in cAMP for metabolic transitions such as the diauxic phase to occur.
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Article Abstract:
Msn2p and partly redundant factor Msn4p are important regulators of stress-responsive gene expression in Saccharamyces cerevisiae. They are necessary for transcription of various genes coding for proteins with stress-protective functions. The two proteins are Cys2His2 zinc finger proteins. They bind to the stress-response element (STRE) and accumulate in the nucleus if there are conditions of stress. C2H2 zinc finger protein Msn2p's localizing in the nucleus is regulated by two things, protein kinase A activity and stress.
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