Article Abstract:
Southern blot hybridization experiments indicate a homology between the aromatic hydrocarbon-degrading Sphingomonas deep-subsurface and surface strains. The utilization of cloned genes for aromatic hydrocarbon degradation from S. yanoikuyae B1 reveals a single hybridization pattern for two surface strains while the deep-subsurface strains have different patterns. Pulsed-field gel electrophoresis separation of genomic DNA indicates that the cross-hybridization genes are present on the plasmids in deep-subsurface strains and in the chromosomes in the surface strains.
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Article Abstract:
There is no similarity between the Comamonas testosteroni genes that degrade aromatic hydrocarbons and the naphthalene degradation genes from Pseudomonas putida. The C. testosteroni genes have a unique organization. Two of the three sample phenanthrene degrading strains of C. testosteroni grow on naphthalene also, and one grows on anthracene. The phenanthrene degrading genes of strain G39 are similar to those of G38A and dissimilar from those of G42. This indicates that there are two different sets of genes for the degradation of phenanthrene in the three strains.
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Article Abstract:
The genes of Pseudomonas pickettii YH105, responsible for the degradation of p-nitrobenzoate, are present on a 43-kb partial MboI fragment. The gene encoding p-nitrobenzoate reductase is present on a 1.4-kb SalI-SphI fragment, while that encoding hydroxylaminolyase is present on a 0.6-kb ApaI-SphI fragment. The two genes are adjacent to each other. The bioreduction of p-nitrobenzoate involves the formation of p-hydroxylaminobenzoate and protocatechuate.
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