Homogeneous expression of the P (sub)BAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter

Article Abstract:

Research has been conducted on the expression systems developed to regulate genes under control of the arabinose-induced araBAD promoter (P (sub)BAD). Results indicate that the expression of araE from the different strength promoters on the medium-copy plasmids or on the chromosome allows homogeneous expression from P (sub)BAD.

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Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-component regulatory system

Article Abstract:

Research has been conducted on the UhpT sugar-phosphate transporter expression in Escherichia coli. Results indicate the glucose-6-phosphate-induced signal transfer through the UphBC complex in the in vitro system which causes UhpB autophosphorylation and phosphoryl transfer to UhpA.

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Deletion of one of two Escherichia coli genes encoding putative Na (super)+ /H (super)+ exchangers (ycgO) perturbs cytoplasmic alkali cation balance at low osmolarity

Article Abstract:

Research has been conducted on the genes in the Escgerichia coli genome similar to the genes encoding eukaryotic Na (super)+ /H (super)+ exchangers. The role of these E. coli genes in bacterial physiology has been investigated and the results are discussed.

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