Article Abstract:
The central fermentation metabolism of Escherichia coli can be redirected to the production of D-lactate or R-lactate. This was gleaned from a study of metabolic engineering of fermentation pathways in E coli for production of optically pure D-lactate, an indigenous fermentation product, or L-lactate, a nonindigenous fermentation product. A pta ppc mutant was able to metabolize glucose exclusively to D-lactate under anaerobic conditions, while a pta ldhA strain was able to metabolize glucose to L-lactate as the major fermentation product.
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Article Abstract:
The main fatty acids present in ten analyzed strains of lactic acid bacteria are palmitic, cis-vaccenic and lactobacillic acid. Most of the Lactobacillus and Lactococcus strains are unable to produce oleic acid. Some strains do not synthesize lactobacillic acid, though all the strains produce dihydrosterculic acid. Lactobacillic acid constitutes 21 to 31% of the total fatty acid concentration of Lactobacillus fermentum and L. buchneri.
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Article Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is used for the detection of bacteriocins. A 30-s water wash of the sample on the probe is shown to be effective in removing contaminants of the analyte. The approach is utilized for rapid detection of pediocin, nisin, enterocin A and B and brochocin A and B from culture supernatants and for the detection of enterocin B throughout the purification process.
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