Article Abstract:
Accurate expression of some genes in yeast depends on the transcriptional coactivator Gcn5p. Several transcriptional regulators, Gcn5p among them, have intrinsic histone acetyltransferase (HAT) activity in vitro. Whether HAT activity of any of the proteins is necessary for gene activation has not been certain. HAT activity of Gcn5p has been found to be essential for transcription activation of target genes in vivo. In vivo data indicate that promoter-specific histone acetylation catalyzed by functional Gcn5p has an essential role in gene activation.
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Article Abstract:
The TG telomere repeat sequences of Saccharomyces cerevisiae confers efficient segregation of plasmidscontaining the sequence (TRS plasmids) during mitosis. However, H4 histone mutation abolishes this efficiency indicating that the chromatin structure is involved in the segregation process. Although, Sir1, Sir2, Sir3 and Sir4 are necessary for transcription of the genes in the sequence, and to some extent, the segregation of the TRS plasmids, mutation in these genes do not completely abolish segregation efficiency.
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Article Abstract:
Chromatin assembly factor I (CAF-I) mutants establish the role of this complex in maintaining silencing at the yeast silent mating loci. The complex does not reestablish silencing as that is done by sir3 cells. This suggests that silencing has two cooperative mechanisms controlling establishment and maintenance.
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