High-throughput screen for poly-3-hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803

Article Abstract:

The development of two novel methods for detecting poly-3-hydroxybutyrate (PHB) based on Nile red fluorescence that are both quantitative and maintain cell viability is described. The straining protocols are novel techniques that are useful in the high-throughput evaluation of combinatorial libraries of Synechocystis and Escherichia coli by using fluorescence-activated cell sorting to identify mutants with increased PHB-accumulating properties.

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A transposition-induced mutant of Nostoc ellipsosporum implicates an arginine-biosynthetic gene in the formation of cyanophycin granules and of functional heterocysts and akinetes

Article Abstract:

An experiment was conducted to analyze a transposon mutant NE1 of Tn5-1058-mutagenized Nostoc ellipsosporum. The N. ellipsosporum was characterized by abnormal akinetes and heterocysts as well as inadequate cyanophycin granules. Test results revealed the formation of cyanophycin granules due to the combination of akinetes and nitrate. In addition, the mutation of a newly identified gene was observed.

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Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli

Article Abstract:

The cyanophycin (CGP) synthetase gene (cph[A.sub.NE1]) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum is cloned and expressed in Escherichia coli strain TOP10. The results have shown that CphA from Nostoc ellipsosporum NE1 has much higher substrate specificity than other cyanobacterial CphAs.

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