High-temperature-induced transposition of insertion elements in Burkholderia multivorans ATCC 17616

Article Abstract:

The transposition of various insertion sequence (IS) elements in Burkholdria multivorans ATCC 17616 is analyzed by using an efficient and quantitative method. pGEN500, a plasmid carrying a Bacillus subtilis-derived sacB gene, was introduced into ATCC 17616 cells, and 25% of their sucrose-resistant derivatives were found to carry various IS elements on pGEN500.

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Functional analysis of unique class II insertion sequence IS1071

Article Abstract:

A study was conducted on many catabolic plasmids often flanked by two copies of insertion sequence, IS1071, which were influenced by various xenobiotic-degrading genes. It was observed that only transposition at high frequencies was possible by IS1071 sequence and that IS1071had the functional features of the class II transposons.

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Complete nucleotide sequence of an exogenously isolated plasmid, pLB1, involved in [gamma]-hexachlorocyclohexane degradation

Article Abstract:

The application of the exogenous plasmid isolation technique to isolate a gene for LinB activity from hexachlorocyclohexane (HCH)-contaminated soil is reported. The report presents a snapshot of the dynamism of the linB gene in the natural environment.

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