High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of campylobacter jejuni

Article Abstract:

A method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. The investigation shows that the CRISPR high-resolution melt (HRM) assay provides a powerful addition to existing Campylobacter jejuni genotyping methods.

author: Smith, Helen, Giffard, Philip M., Price, Erin P., Huygens, Flavia
Genotype, Genotypes

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Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters: assay development and analytical validation

Article Abstract:

Research has been conducted on food-borne thermotolerant campylobacters. The authors have investigated PCR assays for detection of Campylobacter jejuni, C. coli and C. lari in foods, and they report the results.

author: Lubeck, P. S., Wolffs, P., On, S. L. W., Ahrens, P., Radstrom, P., Hoorfar, J.
Denmark, Sweden, Environmental aspects, Microbiology, Microbial populations, Thermochemistry, Microbial ecology

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Enrichment followed by quantitative PCR both for rapid detection and as a tool for quantitative risk assessment of food-borne thermotolerant campylobacters

Article Abstract:

A study is conducted for the first time to detect food borne thermotolerant campylobacters in chickens. The methods and the results are discussed.

author: Hoorfar, J., Josefsen, M. H., Jacobsen, N. R.
Food, Chickens

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subjects list: Research, Analysis, Physiological aspects, Genetic aspects, Campylobacter, Polymerase chain reaction, Food microbiology
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