High-pressure inactivation and sublethal injury of pressure-resistant Escherichia coli mutants in fruit juices

Article Abstract:

Research was conducted to investigate the ability of high-pressure-resistant Escherichia coli mutants to survive high-pressure pasteurization in low-pH buffers and in three different fruit juices. Survival rates were found to be similar for all strains and they correlate positively with juice pH and negatively with temperature. Results indicate that refrigeration enhances the survival of E coli in acidic environments which may be attributed to a reduced permeability of the cell membrane to protons and/or a reduced metabolic activity.

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A simple filtration technique to detect enterohemorrhagic Escherichia coli O157:H7 and its toxins in beef by multiplex PCR

Article Abstract:

A simple two-step filtration approach is proposed for enterohemorrhagic Escherichia coli O157:H7 detection in beef through multiplex PCR. The method addressed the inhibition of the PCR by food particles and unknown metabolic byproducts of bacteria. The approach was found to be more effective than other assays in detecting and characterizing Escherichia coli O157:H7 directly from food.

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Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli

Article Abstract:

Researchers describe the properties of two 2,5-diketo-D-gluconate reductases from E. coli. The enzymes reduce 25KDG to 2-keto-L-gulonate.

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