Article Abstract:
A study has been conducted to describe the development of an expression vector for leptin and optimization in induction as related to cell optical density during high-cell density cultivation of recombinant Escherichia coli for the production of gram quantities of recombinant human leptin. Leptin has been identified as a protein secreted by fat cells that influences body weight homeostasis. This study aims to exploit leptin as a regulator of a therapeutic agent to control human obesity.
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Article Abstract:
Research discusses genetic approaches toward production enhancement of useful proteins in bacteia as exemplified by the overproduction of human leptin in Escherichia coli. The methodology involves proteome profiling of recombinant E. coli during the overproduction of leptin. The productivity of leptin is achieved by manipulating the cysK gene that has the potential to enhance the production of serine-rich proteins as well.
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Article Abstract:
A 'second-generation' production strain was derived from a Corynebacterium glutamicum pantothenate producer by rational design to assess its potential to synthesize and accumulate the vitamin pantothenate by batch cultivation. The strategy successfully improved pantothenate production by genetically modified Corynebacterium glutamicum strains and revealed new constraints in attaining high productivity.
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