High-level acetaldehyde production in Lactococcus lactis by metabolic engineering

Article Abstract:

The efficient rerouting of pyruvate towards acetaldehyde in Lactococcus lactis by functional expression of pyruvate decarboxylase, originating from the gram-negative eth-anologenic bacterium Zymomonas mobilis is described. In resting cells, almost 50% of the glucose consumed could be redirected towards acetaldehyde by combined overexpression of pdc and nox under anaerrobic conditions.

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Production of dehydroamino acid-containing peptides by Lactococcus lactics

Article Abstract:

Nisin, a pentacyclic peptide antibiotic, contains dehydroresidues and thioether rings that are introduced by membrane-associated enzyme complex composed of a serine and threonine dehydratase NisB and the cyclase NisC. The potential of lactococcus lactics expressing NisB and NisC are studied to produce peptitides whose serines and threonines are dehydrated.

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Gene targeting in the gram-positive bacterium Lactococcus lactis, using various delta ribozymes

Article Abstract:

A study was conducted on the use of two variants of the trans-acting anti-genomic delta ribozyme targeting the abundant EF-Tu mRNA in the industrially important gram-positive bacterium Lactococcus lactis. The results showed the high stability in vivo, of the two variants, when expressed at significant levels.

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