High-frequency transfer of a naturally occurring chromosomal tetracycline resistance element in the ruminal anaerobe Butyrivibria fibrisolvens

Article Abstract:

Microbiological research has established the presence of a transmissible chromosomal element with tetracycline resistance in the obligately anaerobic rumen bacterial species Butyrivibrio fibrisolvens. Pulsed-field gel electrophoretic analysis demonstrates the transconjugant's altered chromosomal band profile. Evidence suggests that a distantly related Tc(super r) genetic determinant is transferred.

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Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences

Article Abstract:

Results demonstrate that all three esterase domains share amino acid sequence identity between them, exhibit dockerin-like regions, and catalytic domains. Data further indicate that dockerin from two of the esterases differ significantly and perhaps represent distinct specificities to dockerin.

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EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel celluloase-binding module and to another R. flavefaciens protein via a dockerin domain

Article Abstract:

The properties of the 808-amino acid cellulase produced by the endB gene in Ruminococcus flavefaciens 17 are described. The EndB protein may bind to celluloase via a cellulose-binding module or via its involvement in a cellulosome-like multienzyme complex.

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