Heat susceptibility of aquatic mycobacteria

Article Abstract:

The heat susceptibilities of different opportunistic mycobacteria frequently isolated from water was measured. Survivor measurements were taken at 50, 55, 60 and 70 degrees celsius. The results showed that Mycobacterium kansasii is the most thermosensitive of the mycobacteria studied, followed by Legionella pneumophila Mycobacterium fortuitum, M. intracellulare and M. marinum are similar to L. pneumophila, while M. avium, M. chelonae, M. phlei, M. scrofulaceum and M. xenopi are more thermotolerant than L. pneumophila. Therefore thermal measures against L. pneumophila will not be effective against the last five species.

author: Schulze- Robbecke, Roland, Buchholtz, Katrin
Physiological aspects, Microorganisms, Mycobacteria, Mycobacterium, Temperature effects

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Model system using coliphage phi-X174 for testing virus removal by air filters

Article Abstract:

A method for challenging air filters with aerosols of coliphage phi-X174 was developed. This method was used to compare the removal efficiencies of a prototype and a commonly used air filter. The Dominick Hunterborosilicate glass filter, a commonly used filter, was able to reduce phage concentrations by at least eight orders of magnitude in both long-term and short-term tests. A prototype low-flux Permea membrane gave similar results. However, a Permea high-flux unit delivered a reduction of only five orders of magnitude in short-term tests.

author: Rapp, Martin L., Thiel, Teresa, Arrowsmith, Robert J.
Air filters

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In situ inactivation of animal viruses and a coliphage in nonaerated liquid and semiliquid animal wastes

Article Abstract:

Examination of the persistence of five animal viruses and the coliphage f2 exposed to various animal wastes by filter sandwich technique reveals that storage under nonaerated conditions increase the persistence for prolonged periods of time. Type of animal waste, pH and temperature influence the time required for the reduction of virus titer. Liquid cattle manure induces faster inactivation in virus than that by semiliquid wastes. Experiments help identify the virucidal agents contributing to overall inactivation.

author: Pesaro, Friederike, Sorg, Ingrid, Metzler, Alfred
Viruses, Virus inactivation

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subjects list: Research, Sanitary microbiology, Bacteriophages
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