Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity

Article Abstract:

Aphanothece halophytica, a halotolerant alkaliphillic cyanobacterium that can grow in media of up to 3.0 M NaCl and pH 11 can synthesize betaine from glycine by three-step methylation using S-adenosylmethionine as a methyl donor. The finding show that A-halophytica contains a [Na.sup.+]-betaine symporter that contributes to the salt stress tolerance at alkaline pH.

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Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rps12 gene that allows selection of mutants free from drug resistance markers

Article Abstract:

Research outlines a methodology that enables introduction of multiple gene replacements into the obligate photoautotrophic cyanobacteria. Result show that the streptomycin marker rps12 gene of Synechococcus sp PCC 7942 can be used to modify a specific gene in the recipient strain and to replace any externally introduced drug resistance markers.

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Establishment of a pure culture of the hitherto uncultured unicellular cyanobacterium aphanothece sacrum and phylogenetic position of the organism

Article Abstract:

A speculation is made on the composition of the inorganic elements found in dry aphanothece sacrum which reflects its specific nutritional requirements. Here, a novel synthetic media is designed which is suitable for the growth of this sacrum.

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