Growth temperature dependence of channel size of major outer-membrane protein (OprF) in psychotrophic Pseudomonas fluorescens strains

Article Abstract:

The biochemical function of Pseudomonas fluorescens strain OprF outer-membrane protein was analyzed to determine the mechanisms that regulate outer-membrane permeability in the microbe. Analysis of Pseudomonas fluorescens OprF indicated the role of growth temperature in modulating the pore structure and function of the outer-membrane protein. Furthermore, the channel-forming properties of OprF exhibited little variability in two Pseudomonas fluorescens strains.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane (alk) and naphthalene (nah) catabolic pathways

Article Abstract:

Microbiological research has identified three hydrocarbon-degrading psychrotropic bacteria from Arctic soil samples that contain petroleum contamination. Each isolate appears to belong to the genus Pseudomonas. They can break down alkanes and napthalene via the alk catabolic pathway and the nah catabolic pathway for polynuclear aromatic hydrocarbon biodegradation. Analysis techniques using plasmids shows that the pathways can coexist.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Pore size dependence on growth temperature is a common characteristic of the major outer membrane protein OprF in psychrotrophic and mesophilic Pseudomonas species

Article Abstract:

OprF's from Pseudomonas species of two thermal groups, psychrotrophic P. putide OIG3 isolated from polluted soil and the mesophilic human pathogen P. aeruginosa PAO1 are discussed. Results suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: