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Yeast as a navigational aid in genome analysis

Article Abstract:

Data on the complete genome sequence of brewers' and bakers' yeast was submitted in public data libraries by researchers of the Yeast Genome Project in 1986. The first complete eukaryotic genome sequence was utilized by geneticists to study the biochemical functions of protein-encoding genes based on gene isolation and sequencing techniques. Researchers have also adopted a quantitative approach in the analysis of gene function based on conceptual frameworks involving the characterization of the physical effects of gene deletions and overexpression.

Methods, Genetic aspects, Genomes, Yeast, Yeast (Food product), Genetic research, Microbial genetics

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A gene encoding a thermophilic alkaline serine proteinase from Thermus sp. strain Rt41A and its expression in Escherichia coli

Article Abstract:

The extracellular alkaline serine proteinases produced by thermophile Thermus sp. strain Rt41A is stable at 70 degree celsius for 24 hrs and pH of 8.0. The activation time was reduced by eliminating the hydrophobic signal peptide from the gene. The amino acid sequence of Rt41A was similar to the sequence of Rt41A proteinase which is expressed in Escherichia coli as a fusion protein.

Escherichia coli, Proteins, Enzyme kinetics

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