Article Abstract:
A study on glyceraldehyde-3-phosphate dehydrogenase (gpd) cDNA was conducted to trace whether light-regulated genes are existing in the Trichoderma harzianum bacteria. The methods used in the study include photoinduction and simulated mycoparasitism, protein extraction and antibody production, nucleic acid extraction, and cDNA sequencing. Findings showed that only one gpd is present in Trichoderma harzianum bacteria. Furthermore, the amount of gpd was found to diminish during sporulation. They were also found minimal among mature conidiophores and conidia.
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Article Abstract:
The fungus Trichoderma harzianum is a strong candidate for the biological control of plant diseases since it is antagonistic to other fungi, including plant-pathogenic species. Its biological activity can be further enhanced by genetic manipulation. Previous research on transgenic T. harzianum to obtain increased quantities of specific proteins have been successful. Future studies should concentrate on the production of strains expressing 'multigene' combinations while maintaining the intrinsic vigor and ecological competence of the fungus.
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Article Abstract:
Lectin-carbohydrate interactions during the parasitic activity of Trichoderma harzianum on Sclerotium rolfsii stimulate the production of chitinolytic enzymes in T. harzianum. After the fungi come in contact, CHIT 102 activity increases in T. harzianum which is followed by the appearance of a 1,4-beta-N-acetylglucosaminidase (CHIT 73). The enzymatic changes require de novo formation of the enzymes and the presence of some vital factors in the host S. rolfsii.
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