Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycylic aromatic hydrocarbons

Article Abstract:

Polymerase chain reaction primers isolated from the glutathione S-transferase (GST)-encoding gene of Sphingomonas paucimobilis EPA505 were used in identifying homologs of the GST-encoding gene from samples obtained from New Zealand, Antarctica and the US. The primers amplified the GST from the Sphingomonas strains and were also used to amplify GST from soil DNA. GSTs are genes of detoxifying enzymes that can degrade a wide range of xenobiotic compounds including polycyclic aromatic hydrocarbons.

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Thiocarbamate herbicide-inducible nonheme haloperoxidase of Rhodococcus erythropolis NI86/21

Article Abstract:

The gene encoding the ThcF protein was cloned and sequenced from Rhodococcus erythropolis NI86/21 to determine the mechanisms that mediate the biodegradation of thiocarbamates. The thcF gene encoding the thiocarbamate-inducible protein F exhibited high GC content with a molecular mass of 29,664 kDa. The ThcF protein of the noncardioform Rhodococcus erythropolis also exhibited a protein sequence that was homologous to nonheme haloperoxidase.

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Precipitation of arsenic trisulfide by Desulfotomaculum auripigmentum

Article Abstract:

The As(V)-reducing bacterium, Desulfotomaculum auripigmentum, intra- and extracellularly precipitates As2S3 during the respiration of As(V) to As(III). The precipitation of As2S3 by Desulfotomaculum auripigmentum was dependent on the rate of As(V) reduction. The precipitation of As2S3 was also part of a detoxification mechanism for the removal of As(III) which accumulates in the medium before the formation of As2S3.

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