Glutamate decarboxylase genes as a prescreening marker for detection of pathogenic Escherichia coli groups

Article Abstract:

PCR primers that amplify the genes for glutamate decarboxylase could be used to detect all pathogenic strains of Escherichia coli in water. Researchers show that all strains of E. coli contain these genes, whereas only six Shigella species out of 22 non-E. coli isolates tested had the genes.

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Improved laboratory enrichment for enterohemorrhagic Escherichia coli by exposure to extremely acidic conditions

Article Abstract:

Escherichia coli capable of producing toxins with a cytopathic effect on vero cells were originally designated verotoxin-producing E.coli (VTEC). A procedure was developed for enrichment of enterohemorrhagic escherichia coli by exposure to acidic conditions.

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Multiplex real-time PCR method to identify shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H(sup)- serotype

Article Abstract:

The multiplex real-time polymerase chain reaction (PCR) method for the detection of shiga toxin genes and single-nucleotide polymorphism in the Escherichia coli gene is greater than 98.6% sensitive with 100% specificity.

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