Article Abstract:
Gel electrophoresis analysis of Trichoderma reesei grown on glucose shows that recombinant strains carrying XYN 1 or XYN 2 structural genes are capable of producing the two xylanase isoenzymes, XYN 1 and XYN 2. The total xylanase productivity on xylan is three times that on glucose. However, the specific activities of the two xylanases increase on glucose because of the lesser amount of proteins produced. Electron microscopy and immunogold labelling have detected XYN 2 in the endoplasmic reticulum, Golgi-like vesicles, secretory vesicles, vacuoles, and cell walls.
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Article Abstract:
The two xylanases, CX-I and CX-II, from alkalophilic fungus, Cephalosporium sp. strain RYM-202, have homogeneous characteristics and optimal pHs and optimal temperature. The pH for CX-I is 7.5 and for CX-II is 8.0, and their temperature is 50 degree centigrade. Both enzymes are cellulase-free endoxylanases with transglycosidation activity. They remain stable over 5.5 to 12.0 pHs and exhibit high affinities for less-branched xylans. The xylanases contain similar amino acid compositions with relatively high levels of Gly, Asx and Glx, and little Met.
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Article Abstract:
A total of 160 isolates of Trichoderma from mushroom farms across North America were analyzed to determine their morphological, cultural, and molecular characteristics and to classify them into phenotypic groups. Results revealed that the most common group constituted 40% of the isolates and was identified as a strain of Trichoderma harzianum which causes green mold disease. The strain demonstrated very similar randomly amplified polymorphic DNA patterns as those found in Ireland and the UK.
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