Article Abstract:
An analysis the cAMP-mediated ATP efflux from Saccharomyces cerevisiae was conducted to determine ATP's important role in the physiology of multicellular organisms. Results show that ATP efflux require glucose. Furthermore, the rate of efflux was increased by proton ionophores such as nigericin and monensin as well as by the plasma membrane proton pump inhibitor diethylstilbestrol. Results also indicat that the cAMP pathway triggered ATP efflux and that yeast cells release ATP in a regulated manner.
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Article Abstract:
Intact yeast plasma membranes and reconstructed liposomes were used to investigate the relationship between change in ATPase activity and decomposition of ergosterol by near-UV irradiation. Results suggest that the reduction of ATPase activity in the plasma membrane by near-UV is attributable to conformational changes in the membrane-spanning region of ATPase and not by the photochemical destruction of the primary structure of enzyme molecule.
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Article Abstract:
The reaction of Saccharomyces cerevisiae to benzoic acid was analyzed to determine the energy-dependent mechanisms of resistance of yeasts to weak acid preservatives. Results show that the mechanism of extrusion required energy and prior growth in the presence of the inducers. When energy was available, S. cerevisiae released benzoic acid. On the other hand, inhibitor of ATPases such as diethylstilbestrol prevented benzoic acid intrusion.
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